Epididymal V-Atpase-Rich Cell Proteome Database

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Epididymal V-Atpase-Rich Cell Proteome Database

A comprehensive list of the proteins that are expressed in V-ATPase-rich cells harvested from the epididymides based on the isolation by enzymatic digestion and fluorescence-activated cell sorting (FACS) from transgenic B1-EGFP mice, which express EGFP under the control of the promoter of the V-ATPase-B1 subunit. In these mice, narrow and clear cells of the epididymis express EGFP. The protein identification was performed by LC-MS/MS using an LTQ tandem mass spectrometer (Thermo Fisher Scientific). For questions or comments please contact Sylvie Breton ([email protected]) or Mark A. Knepper ([email protected]).

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METACYC ID Description A0AR23 GO:0004842 (Ubiquitin-Protein Ligase
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Supplemental Material
Supplemental Table B ARGs in alphabetical order Symbol Title 3 months 6 months 9 months 12 months 23 months ANOVA Direction Category 38597 septin 2 1557 ± 44 1555 ± 44 1579 ± 56 1655 ± 26 1691 ± 31 0.05219 up Intermediate 0610031j06rik kidney predominant protein NCU-G1 491 ± 6 504 ± 14 503 ± 11 527 ± 13 534 ± 12 0.04747 up Early Adult 1G5 vesicle-associated calmodulin-binding protein 662 ± 23 675 ± 17 629 ± 16 617 ± 20 583 ± 26 0.03129 down Intermediate A2m alpha-2-macroglobulin 262 ± 7 272 ± 8 244 ± 6 290 ± 7 353 ± 16 0.00000 up Midlife Aadat aminoadipate aminotransferase (synonym Kat2) 180 ± 5 201 ± 12 223 ± 7 244 ± 14 275 ± 7 0.00000 up Early Adult Abca2 ATP-binding cassette, sub-family A (ABC1), member 2 958 ± 28 1052 ± 58 1086 ± 36 1071 ± 44 1141 ± 41 0.05371 up Early Adult Abcb1a ATP-binding cassette, sub-family B (MDR/TAP), member 1A 136 ± 8 147 ± 6 147 ± 13 155 ± 9 185 ± 13 0.01272 up Midlife Acadl acetyl-Coenzyme A dehydrogenase, long-chain 423 ± 7 456 ± 11 478 ± 14 486 ± 13 512 ± 11 0.00003 up Early Adult Acadvl acyl-Coenzyme A dehydrogenase, very long chain 426 ± 14 414 ± 10 404 ± 13 411 ± 15 461 ± 10 0.01017 up Late Accn1 amiloride-sensitive cation channel 1, neuronal (degenerin) 242 ± 10 250 ± 9 237 ± 11 247 ± 14 212 ± 8 0.04972 down Late Actb actin, beta 12965 ± 310 13382 ± 170 13145 ± 273 13739 ± 303 14187 ± 269 0.01195 up Midlife Acvrinp1 activin receptor interacting protein 1 304 ± 18 285 ± 21 274 ± 13 297 ± 21 341 ± 14 0.03610 up Late Adk adenosine kinase 1828 ± 43 1920 ± 38 1922 ± 22 2048 ± 30 1949 ± 44 0.00797 up Early
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Suppl 1.1 All Data Dimethyl Labled
Fasta headers Protein names Gene names Uniprot Ratio H/L normalized exp01 Ratio H/L normalized exp02 Ratio H/L normalized exp03 Ratio H/L normalized exp04 Ratio H/L normalized exp05 Ratio H/L normalized exp06 Ratio H/L normalized exp07 Ratio H/L normalized exp08 Ratio H/L normalized exp09 Ratio H/L normalized exp10 Ratio H/L normalized exp11b Ratio H/L normalized exp12b Peptides exp01 Peptides exp02 Peptides exp03 Peptides exp04 Peptides exp05 Peptides exp06 Peptides exp07 Peptides exp08 Peptides exp09 Peptides exp10 Peptides exp11b Peptides exp12b Unique peptides exp01 Unique peptides exp02 Unique peptides exp03 Unique peptides exp04 Unique peptides exp05 Unique peptides exp06 Unique peptides exp07 Unique peptides exp08 >sp|P01876|IGHA1_HUMAN Ig alpha-1 chain C region OS=Homo sapiens GN=IGHA1 PE=1 SV=2Ig alpha-1 chain C region IGHA1 P01876 1,90 0,62 2,03 12,53 11,35 0,80 1,87 0,60 0,88 1,60 1,33 1,34 7 6 11 8 10 6 8 8 4 3 7 7 3 3 5 4 5 3 4 3 >sp|P02647|APOA1_HUMAN Apolipoprotein A-I OS=Homo sapiens GN=APOA1 PE=1 SV=1;>tr|F8W696|F8W696_HUMAN Truncated apolipoprotein A-I OS=Homo sapiens GN=APOA1 PE=1 SV=1Apolipoprotein A-I;Truncated apolipoprotein A-I APOA1 P02647 1,71 1,13 0,77 1,73 0,89 1,12 0,61 0,84 1,53 1,46 1,16 0,86 17 16 17 18 16 14 13 16 17 13 14 17 17 16 17 18 16 14 13 16 >sp|P01024|CO3_HUMAN Complement C3 OS=Homo sapiens GN=C3 PE=1 SV=2Complement C3;Complement C3 beta chain;Complement C3 alpha chain;C3a anaphylatoxin;Acylation stimulating protein;Complement C3b alpha chain;Complement C3c alpha chain fragment 1;Complement C3dg
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