Analysis of the Active Site Cysteine Residue of the Sacrificial Sulfur

Article Cite This: Biochemistry 2018, 57, 5513−5523 pubs.acs.org/biochemistry Analysis of the Active Site Cysteine Residue of the Sacrificial Sulfur Insertase LarE from Lactobacillus plantarum † ⊥ † ‡ † § † ∥ Matthias Fellner, , Joel A. Rankin, Benoît Desguin, Jian Hu, , and Robert P. Hausinger*, , † Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, United States ‡ Institute of Life Sciences, Universitécatholique de Louvain, B-1348 Louvain-La-Neuve, Belgium § Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, United States ∥ Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824, United States *S Supporting Information ABSTRACT: LarE from Lactobacillus plantarum is an ATP- dependent sulfur transferase that sacrifices its Cys176 sulfur atom to form a dehydroalanine (Dha) side chain during biosynthesis of the covalently linked nickel-pincer nucleotide (NPN) cofactor (pyridinium 3-thioamide-5-thiocarboxylic acid mononucleotide) of lactate racemase. Coenzyme A (CoA) stabilizes LarE and forms a CoA-Cys176 mixed disulfide with the protein. This study presents the crystal structure of the LarE/CoA complex, revealing protein interactions with CoA that mimic those for binding ATP. CoA weakly inhibits LarE activity, and the persulfide of CoA is capable of partially regenerating functional LarE from the Dha176 form of the protein. The physiological relevance of this cycling reaction is unclear. A new form of LarE was discovered, an NPN-LarE covalent adduct, explaining prior results in which activation of the lactate racemase apoprotein required only the isolated LarE. The crystal structure of the inactive C176A variant revealed a fold essentially identical to that of wild-type LarE. Additional active site variants of LarE were created and their activities characterized, with all LarE variants analyzed in terms of the structure. Finally, the L. plantarum LarE structure was compared to a homology model of Thermoanaerobacterium thermosaccharolyticum LarE, predicted to contain three cysteine residues at the active site, and to other proteins with a similar fold and multiple active site cysteine residues. These findings suggest that some LarE orthologs may not be sacrificial but instead may catalyze sulfur transfer by using a persulfide mechanism or from a labile site on a [4Fe-4S] cluster at this position. actate racemase (Lar) interconverts the D and L isomers of from Thermoanaerobacterium thermosaccharolyticum (Uni- L lactic acid, a central metabolite of many microorganisms.1 ProtKB entry D9TQ02) does not require adduct formation.8 The enzyme allows for the production of D-lactate for cell wall This study focuses on LarE, a member of the PP-loop biosynthesis in bacteria that possess only L-lactate dehydrogen- pyrophosphatase family containing a PP-loop SGGxDS motif Downloaded via MICHIGAN STATE UNIV on September 25, 2018 at 12:31:05 (UTC). ase and permits the metabolism of both isomers from racemic in its N-terminal region. Transformation of pyridinium 3,5- mixtures of lactate in microorganisms containing a single form biscarboxylic acid mononucleotide (P2CMN) to pyridinium See https://pubs.acs.org/sharingguidelines for options on how to legitimately share published articles. 2−4 of lactate dehydrogenase. The Lar enzyme from Lactoba- 3,5-bisthiocarboxylic acid mononucleotide (P2TMN) by LarE cillus plantarum includes the protein LarA (UnitProtKB entry requires two cycles of carboxylate activation (involving ATP- F9USS9) and a covalently tethered (via Lys184) cofactor, dependent adenylylation) and sulfur insertion, with the sulfur pyridinium 3-thioamide-5-thiocarboxylic acid mononucleotide originating from a cysteine residue (Cys176) of the protein, with nickel bound to C4 of the pyridinium ring, the two sulfur thus making LarE a sacrificial sulfur transferase that acquires a atoms of the pincer complex, and the His200 side chain 8,9 dehydroalanine (Dha) residue. (Figure 1).5,6 This organometallic complex will subsequently Here, we examine several unusual and interesting features of be termed the nickel-pincer nucleotide (NPN) cofactor.7 The LarE with focus on its Cys176 residue. First, we investigate the NPN cofactor is synthesized from nicotinic acid adenine dinucleotide (NaAD) by the sequential actions of LarB unresolved nonsubstrate interaction of LarE with coenzyme A (UniProtKB entry F9UST0),8 which carboxylates C5 of the (CoA), a cellular component that stabilizes LarE and forms a disulfide with Cys176 but is not believed to be required for pyridinium ring and hydrolyzes the phosphoanhydride bond, 8,9 LarE (UniProtKB entry F9UST4), which converts the two LarE activity, by presenting the crystal structure of LarE with carboxylates to thiocarboxylates,9 and LarC (UniProtKB entry F9UST1), which installs the nickel.8,10 The process by which Received: May 29, 2018 the NPN cofactor becomes covalently attached to LarA from Revised: August 28, 2018 L. plantarum has not been described, and activation of LarA Published: August 29, 2018 © 2018 American Chemical Society 5513 DOI: 10.1021/acs.biochem.8b00601 Biochemistry 2018, 57, 5513−5523 Biochemistry Article pentaerythritol ethoxylate (15/4 EO/OH), 50 mM Bis-Tris (pH 6.5), and 100 mM ammonium sulfate. The CoA-bound sample contained 28 mg/mL WT LarE mixed with 0.9 mM CoA and was incubated for ∼10 min on ice before setting up the drop. The C176A LarE sample contained 8 mg/mL variant protein. Using the same setup that was used for C176A, we also set up unsuccessful crystal drops for W97A (∼1 mg/mL), S180A (∼10, 12, and 15 mg/mL), R212A (∼6 mg/mL), and D231R (∼3, 11, and 24 mg/mL) variants of LarE using the same procedures.9 Diffraction Data Collection, Structure Determination, and Analysis. Data sets were collected at the Advanced Photon Source LS-CAT beamlines (21-ID-F and 21-ID-G). Data sets were processed with xdsapp11 and iMos,12 with merging and scaling done using aimless.13 Phaser molecular replacement14 was utilized using the WT apoprotein model fi Figure 1. NPN cofactor in lactate racemase. Lys184 of LarA binds 5UDQ. Model building and re nement were conducted in Coot15 and Phenix.14 Data set statistics are listed in Table 1. pyridinium 3-thioamide-5-thiocarboxylic acid mononucleotide 16 [shown as sticks with cyan carbon atoms; Protein Data Bank UCSF Chimera was used to create structure figures. (PDB) entry 5HUQ]. Two sulfur atoms and one carbon atom of the Purification of Dha-Containing LarE. LarEDha was cofactor along with His200 coordinate nickel (green sphere). purified from Lactococcus lactis NZ3900 containing pGIR172 that carries the larA−larE genes from L. plantarum with larE bound CoA. Second, we provide evidence that CoA weakly translationally fused to DNA encoding the Strep-tag II5 as inhibits LarE activity. Third, we demonstrate the ability of previously described.8 The cells were grown overnight without CoA-persulfide to regenerate Cys-containing LarE from the being shaken in 15 mL of M17 medium (Oxoid or Difco) Dha Dha176-containing protein (LarE ). Fourth, we provide containing 0.5% (w/v) D-glucose and 10 mg/L chloramphe- evidence of the presence of a novel NPN adduct of LarE, nicol at 30 °C. This inoculum was added to 1.5 L of the same providing an explanation for earlier confounding observations medium, but containing 5 mg/L chloramphenicol, and related to the ability of purified LarE to activate the LarA incubated at 28 °C while being shaken (40 rpm) until the ∼ apoprotein. Fifth, we investigate whether the loss of the OD600 reached 0.3. The culture was supplemented with 1 fi μ Cys176 side chain signi cantly impacts the protein fold by mM NiCl2, induced with 10 g/L nisin A (Sigma), and grown characterizing the structure of the C176A variant. Sixth, we for 4 h before the cells were harvested by centrifugation at characterize the functions of several other key residues of LarE 5000g for 10 min. The cell pellet was washed using 100 mM and summarize all LarE mutagenesis studies to better define Tris-HCl buffer (pH 7.5) containing 300 mM NaCl and stored the sacrificial sulfur insertion reaction.9 Lastly, we speculate until it was needed at −80 °C. The thawed cells were about whether LarE homologues containing additional active suspended in 40 mL of 100 mM Tris-HCl buffer (pH 7.5) site cysteine residues may operate by a catalytic sulfur transfer containing 300 mM NaCl, 1 mM phenylmethanesulfonyl process. fluoride (PMSF, added as a 100 mM stock in ethanol), and 1 unit/mL Benzonase (Millipore) on ice and then lysed by two ■ MATERIALS AND METHODS passes through a chilled French pressure cell at 16000 psi. The Genes, Plasmids, and Cloning. Site-directed mutagenesis debris and intact cells were removed by centrifugation at of the gene encoding Strep II-tagged wild-type (WT) LarE 27000g and 4 °C for 40 min. Purification of LarEDha was from L. plantarum was performed using a QuikChange performed by using a 1 mL bed volume of Strep-tactin-XT mutagenesis kit (Agilent) for creating the K101A, E128A, (IBA) resin equilibrated in 20 mM Tris-HCl buffer (pH 7.5) and E223A variants. All of the constructs were verified by DNA containing 300 mM NaCl at 4 °C. The lysates were loaded at a sequencing. Details, including instructions for creating the rate of 1 mL/min; the resin was washed by gravity flow with C176A variant, are summarized in our previous work.9 five 1 mL additions of 20 mM Tris-HCl buffer (pH 7.5) LarE Overexpression, Purification, and Character- containing 300 mM NaCl, and the protein was eluted with six ization. WT, C176A, and the new variant forms of LarE 0.5 mL additions of 20 mM Tris-HCl buffer (pH 7.5) were overexpressed and purified using our previously described containing 300 mM NaCl and 50 mM biotin (Santa Cruz pGIR076 Escherichia coli ArcticExpress-Strep-tactin (IBA) Biotechnology) that had been neutralized with 50 mM NaOH.

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