Nectopsyche
Neotropical Trichoptera Newsletter ISSN 1550 - 7971 Issue Number 3 June 2006 Examining Trichoptera Genitalia with Confocal Laser Scanning Microscopy Christy Jo Geracy1 & Richard C. Laughlin2 1Department of Entomology, Soils, and Plant Sciences, Clemson University, 2Department of Genetics and Biochemistry, Clemson University Corresponding Address: 114 Long Hall, Box 340315, Clemson University, Clemson, SC 29634 USA, [email protected]
Introduction microscope and compiled into images by computer software. Table of Contents onfocal microscopy is a com- What makes confocal microscopy C Examining Trichoptera Genitalia mon tool that is based on the prin- potentially useful for taxonomy • with Confocal Scanning ciples of fluorescent microscopy. It and systematics is that it takes Microscopy...... 1 has been around for decades, but image slices through the speci- • Editor’s Remarks...... 2 it has mainly been used by cellu- men at user-defined intervals • Colecta, cría y asociación en lar biologists to view intracellular and depths. This “digital dissec- Atopsyche (Collecting, rearing and structures labeled with fluorescent tion” process assures that only the associating Atopsyche, English and dyes. Recently, confocal imaging light that is in focus in any one Spanish)...... 5 has been used for examining in- optical plane is captured. Out-of- • Featured photograph...... 7 ternal morphology and the three- focus background noise, which • New Neotropical Trichoptera Lit- dimensional shape of the cuticle often obscures images taken with erature (2004-June 2006)...... 8 in Blattodea (Periplaneta, Blaberus; conventional light microscopy, is • Being an Entomologist...... 9 Larsen et al. 1997, Zill et al. 2000), eliminated. Each digital slice can • Featured illustration...... 10 and as well as muscle tissue and be examined individually, or the • E-mail List...... 11 internal organs in Diptera (Culex, slices can be compiled and ren- • Researchers, Addresses, Current Drosophila; Klaus et al. 2003). This dered into a three-dimensional Projects...... 12 technique takes advantage of the image that can be rotated about • Announcement 12th International fact that some types of insect tis- the X, Y or Z-axis. This allows the Symposium on Trichoptera...... 13 sue, including most cuticular struc- investigator to see a three-dimen- • Call for Articles...... 14 tures, are autofluorescent under sional representation of the inter- • Call for Subscribers...... 14 red (543 nm) laser excitation. The nal connections within a specimen • On Choosing our Name...... 14 fluorescence is detected by the without physically dissecting it. Page 1 This technique is potentially useful (plastic cover slips cannot be for working with rare specimens, used because they will melt dur- Editor’s Remarks voucher specimens, type mate- ing sample recovery). The mount- rial, or other material that cannot ed structures were allowed to dry It is my pleasure to bring be dissected practically or altered for approximately 30 minutes to you the third issue of Necto- permanently. until solidified. It was important psyche. I apologize for the de- In this report, we used an adult to position the genitalia as close lay in bringing you this issue. I male specimen of Polycentropus to the surface of one of the cover am pleased with the articles in- confusus Hagen as a test species to slips as possible to ensure they cluded herein contributed by measure the autofluorescence of were in the working range of CJ Geraci & Richard Laughlin the caddisfly genitalic structures, the objective. The farther away on confocal microscopy, Paola and to see if the three-dimension- the structure is from the cover Rueda on adult-larva associa- al images produced from confocal slip, the longer the distance the tion, and Ralph Holzenthal on laser scanning microscopy would laser has to penetrate; this can the latest Neotropical publica- contain information useful to tax- decrease resolution (some useful tions. I am very thankful for onomic and phylogenetic studies. tips: press the cover slips together their contributions. Specifically, we wanted to know only slightly -- too much pressure whether this technique can be can flatten and/or damage the ages through the Z-axis was col- used to view internal connections structures; using a label pen to lected (Fig. 2 A-D). The complete between membranous and sclero- circle the structures on the cover- series of image slices was then tized portions of the male phallus. slips makes it easeir to find them used to render a three-dimension- under the microscope). al image of the phallus (Fig. 3). Methods The protocols for mounting Imaging: The male genitalic struc- Specimen Recovery: To remove the structures and taking images with tures were imaged with a Zeiss genitalia from the gelatin solution the Carl Zeiss LSM 510 imaging LSM 510 Confocal Microscope the cover slip mount was placed in software were adapted from Klaus with either the 10X or 40X objec- a glass dish with distilled water and et al. (2003; see reference for de- tive by light or red laser (543nm). heated for approximately 20 min- tailed imaging procedures). To assess whether regions of the utes (low heat). When the solution cuticle were autofluoresent, im- had melted enough for the cover Mounting: The cleared male geni- ages using either the laser of slips to be separated, they were talia of Polycentropus confusus white light were taken (Fig 1, Pan- pulled apart carefully with forceps were mounted between two glass els 1 & 2). Light and laser images in order to avoid tearing the geni- cover slips in a solution of 1:17:17 were then digitally overlayed (Fig. talia. After the top cover slip was (w:v:v) gelatin:glycerine:water 1, Panel 3). Next, a serial set of im- removed the genitalia were con-
Figure 1. Polycentropus confusus, tergum X and genitalia of male. Panel 1: autofluorescence under the red laser (543 nm); Panel 2: bright field image; Panel 3: red fluorescence overlaid onto brightfield image (Carl Zeiss Microscope LSM 510 software, 10X objective). Page 2 tained in a drop of media on the standing connections in any one py is useful for viewing arthropod bottom cover slip. The bottom cov- plane in the z-stack (Figure 2). cuticle and muscle tissue, but does er slip was put back into the dish of The three-dimensional render- not work well with darkened or distilled water and returned to the ing was most useful for view- solid cuticular structures. Confocal heat for approximately another 20 ing internal structures, and the images could be used in addition minutes (enough time to dissolve invagination of the endothecal to illustrations to show diagnostic the media). Finally, the genitalia membrane with its endothecal characters whose three-dimen- were removed from the cover slip spines. Deleting the proximal sional shape is important. The and placed in the distilled water slices exposed the invaginated internal connections between for an additional 30 minutes to endothecal membrane and se- muscle and cuticle that are shown fully dissolve the remaining gela- tae, which could then be rotated in the three-dimensional images tin. (Times are approximate, and about each axis to see where the can be used to help assess homol- may vary depending on the tem- membrane attached to a scleri- ogy for phylogenetic studies. The perature used and the size of the tized tube (Figure 3). most important factor in produc- mounted structures.) ing high quality images is properly Additional observations: We sur Observations and - mounting the structures between veyed various other taxa to test the cover slips. Damage to the Conclusions their levels of autofluorescence. structures may occur if care is not Polycentropus confusus: Carl Muscle tissue in the femur of a taken during the sample recovery Zeiss LSM 510 imaging software baetid mayfly, the peritrophic process, or if the structures are allowed us to view each optical matrix of a simuliid larva, and compressed too much between slice taken at the specified Z-axis the exocuticle of a tardigrade the cover slips. interval and overlay the fluores- all fluoresced brightly. However, cence onto the light image (Figure hardened and darkened cu- Free image analysis software: Carl 1). This showed any structures that ticle was not fluorescent, as has Zeiss LSM Image Browser Ver- were not fluorescent. It is not fully been reported previously (Zill sion 3.5 available for free down- understood why some some struc- et al. 2000). For example, Chi- load from the Carl Zeiss web site tures do not fluoresce while others ronomidae larval mouthparts (http://www.zeiss.com/) do. All portions of the male genita- were fluorescent except for the lia of P. confusus were fluorescent. mandibles, and the head capsule Software for converting LSM Figure 2 shows representative of Micrasema rickeri Ross & Un- files to QuickTime or .mpeg files: slices of the specimen at differ- zicker did not fluoresce. We also ImageJ (Image Process-ing and ent Z-axis coordinates. We found found that setae and other hol- Analysis in Java) available for free the limit for the z-stack was ~150 low structures fluoresced bright- download from the US National um, with a minimum interval size ly, but solid structures did not. Institutes of Health web site (http:// of ~0.5 um. Viewing the individual rsb.info.nih.gov/ij/) image slices was useful for under- Conclusions: Confocal microsco-
Figure 2. Digital slices of the right lateral view of the phallus of Polycentropus confusus taken at sample intervals (40X objective; z-stack range: 0.0 - 118.4 um, 1.6 um interval).
Page 3 References Klaus, A.V., V.L. Kulasekera, and Larsen, G.S., S.F. Frazier, and S.N. Zill, S., S.F. Frazier, D. Neff, L. Quimby, V. Schawaroch. 2003. Three-di- Zill. 1997. The tarso-pretarsal M. Carney, R. Dicaprio, J. Thuma, mensional visualization of insect chordotonal organ as an ele- and M. Norton. 2000. Three-dimen- morphology using confocal laser ment in cockroach walking. Jour- sional graphic reconstruction of scanning microscopy. Journal of nal of Comparative Physiology the insect exoskeleton through Microscopy 212(2): 107-121. 180: 683-700. confocal imaging of endogenous fluorescence. Microscopy Research and Technique 48: 367-384.
Figure 3. Polycentropus confusus, three-dimensional lateral view of right half of phallus and endothecal membrane rotated about the y axis; 40X objective.
Page 4 Colecta, cría y asociación en Atopsyche (Banks) (Trichoptera: Hydrobiosidae) Paola Alejandra Rueda Martín * La asociación larva, pupa y adulto en Trichoptera Association of larva, pupa and adult trichoptera is es uno de los objetivos a los que apuntan muchos de an objective in several of today’s works. The ecologi- los actuales estudios. La importancia ecológica de las cal importance of trichoptera larvae as bioindicators larvas de Trichoptera como bioindicadores es indis- is unquestionable (Basaguren 1988). For this, the im- cutible (Basaguren 1988) por ello es necesario correla- mature stages need to be identified. Several authors cionar los distintos estados de las especies. El método (Vorhies1909; Milne 1938; Ross 1944, Wiggins 1996) del metamorfotipo fue descripto por varios autores described the metamorphotype method, as well as (Vorhies 1909; Milne 1938; Ross 1944;Wiggins 1996), rearing and collecting methods. However, it is essen- al igual que distintos métodos de cría y colecta, pero tial to have a complete sclerotized male genitalia in requiere necesariamente que la genitalia del macho order to use this method. In Tucumán province, Ar- esté completamente esclerotizada. En la provincia de gentina, I am working on the association of larvae and Tucumán, República Argentina, actualmente estoy re- adult species of Atopsyche. The aim of this article is to alizando un trabajo sobre la asociación de larvas del make a contribution on collecting, rear-ing, and asso- género Atopsyche hasta el nivel de especie y el pre- ciation methods of preimaginal stages in the genus sente artículo pretende aportar datos sobre la colecta, Atopsyche (Banks). cría y asociación de los distintos estados del Género Collecting Method: Atopsyche (Banks). Collecting trips to obtain immatures for rearing Método de colecta: need to be short in order to assure the survival of the Las salidas de campo en las que se obtiene materi- animals. Another option is to rear in situ. To collect al para criar deben ser preferentemente cortas, de 1 o larvae, it is necessary to have a plastic strainer and a 2 días para asegurar la supervivencia de los animales, plastic white tray. A very simple optional method to o de lo contrario se deben aplicar metodologías para collect pupae is to search manually by picking up cría in situ. Para la colecta de larvas, el uso de cola- stones from river rapids, especially in the small water-
Page 5 dores plásticos blancos es casi indispensable, la red falls. This methodology is preferable in order to assure D también resulta útil acompañada de una bandeja the survival of pupae for rearing. Atopsyche larvae are plástica blanca. Otro método sencillo y útil para la col- carnivorous and they live in cold water, thus rearing ecta de las pupas es la búsqueda manual, levantando becomes difficult. All larvae collected in the field are piedras en las zonas del río de mayor velocidad y es- fixed in 75% alcohol, whereas the pupae are trans- pecialmente en pequeñas cascadas, ésta metodología ported in aquarium labeled bags. The long distance es necesaria para asegurar que las pupas no sufran transport implies refrigeration of the rearing material. daños que imposibiliten la cría. Dado que las larvas It is also necessary to carry water from the river for the de Atopsyche son carnívoras, y que habitan ríos más aquarium. bien fríos, la cría se dificulta, por ello se fijan en alco- Rearing pupae: hol 75%, mientras que las pupas son transportadas vi- Pupae are examined under a magnifying glass to vas en bolsas de acuario etiquetadas, con poca agua see their condition or vitality. They are then put in in- e infladas con aire; si el tiempo de traslado es largo, dividual rearing containers, which have windows with conviene colocarlas en una conservadora refrigerada. nets to assure water circulation and ensure that after Resulta conveniente llevar al laboratorio una cierta emergence the adults won’t escape. Containers are cantidad de agua del mismo río para ser utilizada en placed in an aquarium in 4 cm of water with an air los acuarios. pump. Water is kept at room temperature because the Cría de las pupas: pupae can tolerate higher water temperatures than Las pupas se examinan previamente en la lupa the larvae. Containers must be checked twice a day. para asegurar que se encuentran en buenas condicio- Pharate adults have no difficulty abandoning the pu- nes, posteriormente se colocan en recipientes de cría pal cocoon, but often can’t free themselves from the individuales, los cuales tienen ventanas con red para pupal cuticle and wings cannot be extended. Pupae asegurar la circulación de agua y que los adultos no se with low vitality are fixed in 75% alcohol for future escapen luego de emerger. Los frascos se colocan en comparison. With this methodology all preimaginal un acuario con 4 cm de agua de río y una bomba de stages and sexes of a species can be associated. aire a temperatura ambiente ya que las pupas pueden Association: tolerar temperaturas mayores que las larvas, lo que Males can be identified to species and the remain- se refleja en un alto porcentaje de supervivencia. Los ing larval sclerites are compared with a previ-ously frascos se revisan 2 veces al día. Al emerger, el adulto collected larvae to obtain association. In order to as- farado se libera del cocón sin problemas, y la genitalia sociate the adult female, female pupae are identified está ya completamente desarrollada, aunque en algu- using the remaining sclerites that correspond to the nos casos la cutícula de la pupa no se desprende com- previously identified larvae. pletamente, y el adulto no puede extender las alas. Las Metamorphotype method: pupas que no son aptas para criar, se fijan en alcohol Fixed pupae are examined under a magnifying 75% para ser comparadas posteriormente. glass cutting the cocoon around the anterior ex-treme - De ésta manera se pueden asociar todos los esta with a needle. The pupa and remaining sclerites (later- - dos de una especie y a partir de la asociación del ma al sclerite of the head, frontoclypeus, pronotum, pros- cho con la larva, se identifica a la hembra utilizando la ternal plates, anterior prolegs, anal prolegs) are taken misma metodología. out. After making the necessary observations (identifi- Asociación: cation of male, illustrations, etc.), the sclerites, anterior Los machos adultos farados que emergen pueden extreme of the cocoon and the pupa are introduced ser identificados hasta el nivel de especie, luego se into the empty cocoon, and in this way several pupae comparan los escleritos remanentes de la larva con can be stored in the same container. las larvas colectadas en el campo y se realiza la aso- The use of the rearing and metamorphotype ciación. Para asociar las hembras se procede identifi- methods permits precise associations. The mate- cando las pupas a partir de los escleritos de la larva ya rial can be identified to species in all cases, includ- asociada al macho. ing prepupae by comparing previously associated sclerites. Page 6 Método del metamorfotipo: Referencias: Los machos adultos farados que emergen pueden Basaguren, A.1988. Tricopteros como indicadores de la ser identificados hasta el nivel de especie, luego se calidad de las aguas de Bizkaia. Univ. Del Pais Vasco, Fac. comparan los escleritos remanentes de la larva con de Ciencias, Laboratorio de Ecología. Apartado 644.48080 las larvas colectadas en el campo y se realiza la aso- Bilbao España. ciación. Para asociar las hembras se procede identifi- cando las pupas a partir de los escleritos de la larva ya Milne, M.J. (1938) The “Metamorphotype Method” in Tri- asociada al macho. choptera. Journal New York Entomological Society, XLVI, Combinando el método de cría y el de metamor- 435-437. fotipo, se pueden realizar asociaciones seguras Ross, H. H. 1944. The Caddis flies, or Trichoptera, of Illinois. y además posteriormente es posible reconocer Illinois Natural History Survey Bulletin, 23(1):1-326. hasta el nivel de especie, pupas que no están en un desarrollo completo por comparación de los es- Vorhies, C. T. 1909. Studies on the Trichoptera of Wisconsin. cleritos de la larva junto con la genitalia aunque Transactions of the Wisconsin Academy of Science, Arts and no esté totalmente esclerotizada. Letters 16: 647-738. * FONCYT- INSUE, Tucumán, Argentina Wiggins, G. B. 1996. Larvae of the North American Caddisfly Address correspondence to: Paola A. Rueda Martín,. Miguel Lillo Genera (Trichoptera). Second Edition. 457 pages. Toronto 205-CP 4000-San Miguel de Tucumán. Tucumán. Argentina. E-mail: [email protected] and Buffalo: University of Toronto Press.
Campamento Mataracú, Amboró National Park, Bolivia. Photo Courtesy of Desiree Robertson. Page 7 New Neotropical Trichoptera Literature (2004-May, 2006) Ralph W. Holzenthal This list is meant to supplement and update the bibliography cited in the Catalog of the Neotropical Cad- disflies (Flint et al 1999) and the literature updates in issues 1 and 2 of Nectopsyche. The former work contained a list of literature through the end of 1998, but included some works published in early 1999. Users should con- sult the Catalog for a comprehensive list of Neotropical taxa and associated bibliographic citations
Angrisano, E.B, and J.V. Sganga. 2005. Contribution to Botosaneanu, L., and A. Thomas. 2004(2005). Nou- the knowledge of the genus Metrichia Ross from Ar- velles contributions a la connaissance des Trichopter- gentina (Trichoptera: Hydroptilidae: Ochrotrichiini). es de Martinique, avec description de deux especes Aquatic Insects 27(2): 113-123. nouvelles (Trichoptera). Ephemera 6(1): 33-58.
Barba-Alvarez, R., and J. Bueno-Soria. 2005. New spe- Bravo, W., and E. Angrisano. 2005. Contribution to the cies of the genus Polycentropus Curtis (Trichoptera: knowledge of the South American Hydrobiosidae: the Polycentropodidae) from Mexico. Proceedings of the larvae of Clavichorema and Pseudoradema (Trichop- Entomological Society of Washington 107(3): 663- tera). Folia Entomologica Mexicana 44(1): 19-26. 670. Bravo, W.R., and E.B. Angrisano. 2001(2004). The pre- Bispo, P.C., L.G. Oliveira, V.L. Crisci-Bispo, and K.G. Sousa. imaginal stages of Pomphochorema chilense Flint 1969 2004. Environmental factors influencing distribution (Trichoptera, Hydrobiosidae). Physis Secciones A B y C and abundance of trichopteran larvae in central Bra- (Buenos Aires) 59(136-137): B37-B41. zilian mountain streams. Studies on Neotropical Fau- na and Environment 39(3): 233-237. Bueno-Soria, J. 2004. Trichoptera (Insecta). Pages 171- 179. In, A.N. Garcia Aldrete and R. Ayala Barajas [Eds]. Blahnik, R.J., and R.W. Holzenthal. 2006. Revision of Artropodos de Chamela. Instituto de Biologia, UNAM, the genus Culoptila (Trichoptera: Glossosomatidae). Mexico City. i-xi, 1-227. Zootaxa 1233: 1-52. Bueno-Soria, J., and A. Rojas-Ascencio. 2004. New Blahnik, R.J. 2005. Alterosa, a new caddisfly genus species and distribution of the genus Marilia Müller from Brazil (Trichoptera: Philopotamidae). Zootaxa (Trichoptera: Odontoceridae) in Mexico and Central 991: 3-60. America. Proceedings of the Entomological Society of Washington 106(3): 679-696.
Page 8 Bueno-Soria, J., S.Santiago-Fragoso, and R.Barba-Al- Holzenthal, R.W., and R.J. Blahnik. 2006. The caddisfly varez. 2004. More new Trichoptera from Mexico and genus Protoptila in Costa Rica (Trichoptera: Glossoso- Panama. Transactions of the American Entomological matidae). Zootaxa 1197: 1-37. So-ciety (Philadelphia)130(4): 479-486. Huamantinco, A.A., and J.L.Nessimian. 2004. A new Calor, A.R., R.W. Holzenthal, and D.S. Amorim. 2006. Neotropical genus and species of Odontocerinae (Tri- Phylogenetic analysis of Notalina (Neonotalina) Hol- choptera: Odontoceridae) from southeastern Brazil. zenthal (Trichoptera: Leptoceridae), with the descrip- Aquatic Insects 26(3-4): 281-288. tion of two new species from southeastern Brazil. Zootaxa 1131: 33-48. Huamantinco, A.A., L.L. Dumas, and J.L. Nessimian. 2005. Description of larva and pupa of Phylloicus ab- Cohen, S.A. 2004. Caddisflies from the Instituto-Fun- dominalis Ulmer, 1905 (Trichoptera: Calamoceratidae). dacion Miguel Lillo (Province of Tucuman, Argentina). Zootaxa 1039: 19-26. Acta Zoologica Lilloana 48(1-2): 73-80. Johanson, K.A. and T. Malm. 2006. Seven new Heli- Crisci-Bispo, V.L., P.C. Bispo, and C.G. Froehlich. 2004. copsyche (Feropsyche) Johanson, 2002 from the Neo- Triplectides larvae in empty cases of Nectopsyche (Tri- tropical region and Nearctic Mexico (Insecta: Trichop- choptera, Leptoceridae) at Parque Estadual Intervales, tera: Helicopsychidae). Zootaxa 1208: 1-24. São Paulo State, Brazil. Revista Brasileira de Entomolo- gia 48(1): 133-134. Lopez del Castillo, P., C. Naranjo Lopez, J.L. Fernandez Triana, D. Gonzalez Lazo, A. Trapero Quintana, and J. Gonzalez Lazo, D.D., A. Trapero Quintana, C. Naranjo Perez Ozoria. 2004. The aquatic insects of the Na- Lopez. 2005. Insectos acuaticos del Parque Nacional tional Park “La Bayamesa”, Cuba. Boletin de la SEA 35: “Alejandro de Humboldt”, Cuba. Boletin de la SEA 36, 225-231. 30: 257-261. Medillin C., F., M. Ramirez O., and M.E. Rincon. 2004. Hamilton, S.W., and R.W. Holzenthal. 2005. Five new Trichoptera of he [the] Santuario de Iguaque (Boyaca, species of Polycentropodidae (Trichoptera) from Ec- Colombia) in relation to water quality. Revista Colom- uador and Venezuela. Zootaxa 810: 1-14. biana de Entomologia 30(2): 197-203.
Wow! The genitalia looks just like a polycen- tropodid, but it has ocelli. Perhaps this is an interesting case of speciation across lineages through introgression. The result could be the formation of intermediate or introgressed popula- tions which, because of their hybrid origin also had Being an Entomologist increased genetic variance, most relevantly for characters relating to the structures of the head, but quite possibly also by Lourdes Chamorro-Lacayo & Ralph Holzenthal for unobserved characters, such as pheremonal, behavioral or ecolocigal traits. Newly acquired differences developed in these populations may have been sufficient to allow them to become effectively self discriminating and genetically isolating, thus also Oh... wait... Wow! This providing the means for the populations that’s not the body rhyacophilid to become extensively sympatric. Probably some ecological differentiation for this genitalia... genitalia I just cleared accompanied this I must have switched is really weird. sympatrization... them... Hey, Rog, come take a look!
Another beautiful theory destroyed by an ugly little fact.
Ralph
Page 9 Moretti, M.S., and R.D. Loyola. 2005. Does Barypenthus Rueda Martin, P.A. 2005. Larva, pupa and adult male of concolor Burmeister (Trichoptera : Odontoceridae) se- Atopsyche (Atopsyche) maxi, a new species from north- lect particles of different sizes for case building? Neo- western Argentina and southern Bolivia (Trichoptera: tropical Entomology 34(2): 337-340. Hydrobiosidae) Aquatic Insects 27(4): 293-298.
Naranjo Lopez, C., and D.D. Gonzalez Lazo. 2005. Situa- Schefter, P.W. 2005. Re-evaluation of genera in the cion actual del estudio del orden Trichophera en Cuba. subfamily Hydropsychinae (Trichoptera: Hydropsy- Boletin de la SEA 36, 30: 147-152. chidae). Aquatic Insects 27(2): 133-154.
Pes, A.M.O., and N. Hamada. 2004. Ceratotrichia Flint, Shuvartz, M., L. Goncalves Oliveira, J.A.F.Diniz-Filho, 1992 (Trichoptera: Hydroptilidae) larval and pupal de- and L.M. Bini. 2005. Relaçoes entre distribuição e scription and new genus records for Brazil. Entomo- abundancia de larvas de Trichoptera (Insecta), em cor- tropica 19(1): 31-37. regos de Cerrado no entorno do Parque Estadual da Serra de Caldas (Caldas Novas, Estado de Goias). Acta Pes, A.M.O., N.Hamada, and J.L. Nessimian. 2005. Scientiarum Biological Sciences 27(1): 51-55. Chaves de identificaco de larvas para familias e gener- os de Trichoptera (Insecta) da Amazonia Central, Brasil. Villanueva, V.D., and C. Trochine. 2005. The role of mi- Revista Brasileira de Entomologia 49(2): 181-204. croorganisms in the diet of Verger cf. limnophilus (Tri- choptera: Limnephilidae) larvae in a Patagonian An- Robertson, D.R., and R.W. Holzenthal. 2005. The Neo- dean temporary pond. Wetlands 25(2): 473-479. tropical caddisfly genus Tolhuaca (Trichoptera: Glos- sosomatidae). Zootaxa 1063: 53-68.
Illustration courtesy of Ralph Holzenthal
Page 10 E-mail List
AGUILA, Yolanda [email protected] Panama ALVAREZ, Luisa [email protected] Colombia ANDERSEN, Trond [email protected] Norway ANGRISANO, Elisa [email protected] Argentina ARAYA, Ely [email protected] Chile ARMITAGE, Brian [email protected] USA BARBA, Rafael [email protected] Mexico BLAHNIK, Roger [email protected] USA BONADA, Nuria [email protected] Spain BUENO-Soria, Joaquin [email protected] Mexico CALOR, Adolfo [email protected] Brazil CHAMORRO-Lacayo, Ma. Lourdes [email protected] USA/Nicaragua CIANFICCONI, Fernanda [email protected] Italy CONTRERAS-Ramos, Atilano [email protected] Mexico CORDEIRO, James [email protected] USA CRESSA, Claudia [email protected] Venezuela de ALMEIDA, Gisele [email protected] Brazil ENCALADA, Andrea [email protected] USA FROEHLICH, Claudio [email protected] Brazil FLINT, Oliver fl[email protected] USA OLIVEIRA, Leandro [email protected] Brazil GALLARDO, Marina [email protected] Colombia? GERACI, C.J. [email protected] USA GONZALEZ, Marcos [email protected] Spain GUEVARA Cardona, Giovany [email protected] Colombia HAMILTON, Steve [email protected] USA HARRIS, Steve [email protected] USA HOLZENTHAL, Ralph [email protected] USA HUAMANTINCO, Ana [email protected] Brazil JOHANSON, Kjell A. [email protected] Sweden KJER, Karl [email protected] USA LOPEZ del Castillo, Pedro [email protected] Cuba MATHURIAU, Catherine [email protected] Brazil? MELO, Adriano Sanchez [email protected] Brazil MENA, Ramón [email protected] Colombia MONESTE, Heidi [email protected] Costa Rica MONSON, Margot [email protected] USA MORSE, John [email protected] USA MOULTON, Steve [email protected] USA MUÑOZ-Quesada, Fernando [email protected] Colombia NESSIMIAN, Jorge [email protected] Brazil OGBOGU, Sylvester [email protected] Nigeria
Page 11 E-mail List
PAPROCKI, Henrique [email protected] Brazil PARYS, Katherine [email protected] USA PES, Ana Maria Oliveira [email protected] Brazil POSADA, Jose Andres [email protected] Colombia PRATHER, Aysha [email protected] USA PUIG, M. Angels [email protected] Spain RASMUSSEN, Andrew [email protected] USA ROBERTSON, Desiree [email protected] USA/Bolivia RUEDA Martin, Paola [email protected] Argentina RUIZ García, Antonio [email protected] Spain SANABRIA, Maria Judith [email protected] Holland/Colombia SMITH, Brian [email protected] New Zealand SPRINGER, Monica [email protected] Costa Rica TACHET, Herni [email protected] France Brazil UIEDA, Virginia [email protected] Spain VIEIRA Lanero, Rufino [email protected] USA WASMUND, Anne [email protected] USA WEAVER, John [email protected] Colombia ZUÑIGA de Cardoso, M. del Carmen [email protected]
Researchers, Addresses & Current Projects
James Cordeiro Antonio Ruiz García Research Zoologist Universidad Pablo de Olavide. Departamento de NatureServe Ciencias Ambientales (Zoología). 11 Avenue de Lafayette, 5th Floor 41013. Sevilla. Spain. Boston, MA 02111 [email protected] [email protected] His research interests are larval taxonomy and His research deals with North American ecology. Trichoptera distribution. He is the Central Data- base coordinator to U.S. Natural Heritage Net- work and Canadian Conservation Data Centres for caddis- flies (and other aquatic invertebrates); responsi- ble for assigning global and national rarity ranks (Grank system).
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12 th International Symposium on Trichoptera Mexico, D.F., June 18-22, 2006
Dear Colleagues, It is my pleasure to invite you to the XII International Symposium on Trichoptera, in Mexico City. This symposium will take place at the Instituto de Biología, located at Universidad Nacional Autónoma de México (UNAM) Campus from June 18 to 22, 2006. Transportation and accommodations: Mexico City International Airport is connected with most airlines of the world; I am sure you will find it very easy to arrive to Mexico City. You must take only the autho- rized airport transportation to your hotel. Accommodations will be available at a Hotel in the Campus vicinity. The prices shown below are the actual prices and are subject to a 10% increment due to inflation:
Hotel Radisson Paraíso: Double Room: $ 96.00 USD ($ 1100.00 pesos) per night, plus tax Single Room: $ 96.00 USD ($ 1100.00 pesos) per night, plus tax As you can see the price is better if you share a double room. Meals are not included and the following are samples of prices for buffet type meals: Breakfast buffet: $ 16.00 USD ($ 187.00 pesos) Dinner buffet: $ 22.00 USD ($ 261.00 pesos) The exchange rate at present is $ 11.25 Mexican pesos per 1 USD.
Registration fee per participant is $ 350.00 USD and $ 200.00 USD for each accompanying person. This price includes the proceeding volume. We hope to receive your pre-registration soon, if possible, with your e-mail address. Please indicate your intention to attend with or without accompanying person(s). I would like to end this note with information about the weather conditions for June: average tem- perature in Mexico City 20oC to 26oC during the day, with cooler evenings. The rainy season starts in May and ends in September. I look forward to seeing you in June, 2006.
Sincerely yours, Joaquín Bueno-Soria Dr. Joaquín Bueno-Soria Apdo. Postal 70-153, México 04510 D. F. FAX: +(5255) 5550-0164 Phone: + (5255)-5622-9158 E-mail: [email protected] Web site: http://www.ibiologia.unam.mx/barra/congresos/entomolo/index.htm. Page 13 Call for Articles If you would like to contribute an article to Necto- Call for Subscribers psyche, send it to the editor ([email protected]) as a Word file or rich text format (rtf) as an attachment by e- mail, on disk or CD by snail mail to: Lourdes Chamorro-L., Editor Department of Entomology embership is free and open to anyone who would Mlike to receive Nectopsyche. Only electronic versions (pdf) are University of Minnesota Nectopsyche 219 Hodson Hall, issued. Additionally, can be downloaded from the 1980 Folwell Avenue, following site: http://www.entomology.umn.edu/museum/ St. Paul, MN 55108. links/news.html Please feel free to update and make correc- We welcome submissions dealing mainly with is- tions to personal information found in this issue. We encourage Nectopsyche sues associated with Neotropical Trichoptera. Nonetheless, colleagues to share with others and look forward articles that fulfill the objectives of Nectopsyche and meet to including more members. This will allow Trichoptera work- the interests and needs of the general readership will be ers to be aware of each other and of their current projects and considered. Spanish, Portuguese, or English versions are hopefully foster collaboration and interaction. Please feel free accepted. Please remember that your contributions make to send the editor your information following the format below: Nectopsyche possible. Pictures are also welcome. 1. Name (SURNAME in uppercase); 2. Mailing Address of workplace; 3. E-mail address; 4. CURRENT RESEARCH, general interests, etc; On Choosing our Name 5. NEWS, NOTES and/or GENERAL INFORMA- Many reasons led us to choose Nectopsyche as the name TION of interest to Neotropical Trichoptera for this Newsletter. Nectopsyche is a genus of New World cadd- colleagues. isflies in the Family Leptoceridae whose members possess vivid hair and scale coloration creating arrangements of intricate patterns on their wings. Nectopsyche characterizes the present caddisfly fauna in the Neotropics in that more than half of the actual diversity is undescribed with further collections general- This material is based upon work sup- ly adding new species. Nectopsyche is also endemic to the Neo- ported by the National Science Foundation tropics with some geographical extension to the North, thus under Grant No. 0117772. Any opinions, find- somewhat paralleling the area of interest encompassed in this ings, and conclusions or recommendations Newsletter. Nevertheless, Nectopsyche serves as the emblem of expressed in this material are those of the this Newsletter primarily because it is striking and charismatic. author(s) and do not necessarily reflect the For this third issue of Nectopsyche we have chosen an il- views of the National Science Foundation. lustration of Nectopsyche multilineata Flint 1983 rendered by Julie Martinez to decorate the banner.
Nectopsyche is a publication of the University of Minnesota Insect Collection. The University of Minnesota is an equal opportunity educator and employer. ©2004 by the University of Minnesota Insect Collection and the Re- gents of the University of Minnesota. All rights reserved.
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