Curriculum Vitae

Steven John Lenhert

January 16, 2020

General Information

University address: Biological College of Arts & Sciences 221A Biology Unit I Florida State University Tallahassee, Florida 32306-4370 Phone: (850) 645-9401; Fax: (850) 644-0481

E-mail address: [email protected]

Web site: http://www.bio.fsu.edu/faculty-lenhert.php

Professional Preparation

2004 Dr. rer. nat., University of Muenster, Germany. Major: Biology. Nanotechnology (Physics/Biology/Medicine). magna cum laude.

2002 Master of Science, University of Muenster, Germany. Major: Biology.

2000 Bachelor of Science, Messiah College, Grantham, Pennsylvania. Major: Biochemistry, mathematics.

Nondegree Education and Training

2010 Entering Mentoring (taught by Dr. Bryant Chase, FSU).

2005–2007 Postdoctoral research at the Institute for Nanotechnology in the Forschungszentrum Karlsruhe, Nanomechanics Group of Professor Harald Fuchs, and Northwestern University, Group of Professor Chad A. Mirkin.

Professional Experience

2016 Associate Professor, Biological Science, Florida State University.

2010–2016 Assistant Professor, Biological Science, Florida State University.

Vita for Steven John Lenhert

2008–2009 Group Leader, Nanomechanics Group of Professor Fuchs, Institute for Nanotechnology in the Forschungszentrum Karlsruhe.

2005–2008 Scientific coworker, INT, Forschungszentrum Karlsruhe, Germany.

2002–2004 Scientific coworker, ZKM, Uni-klinikum Muenster, Germany.

2000–2002 Scientific assistant, AG Fuchs, University of Muenster, Germany.

1999 AFM Research internship (summer), AG Fuchs, University of Muenster, Germany.

Language Proficiency

English - native in speaking, reading, and writing. German - fluent in speaking; advanced in reading; intermediate in writing. Greek - intermediate in speaking; rudimentary in reading and writing. Spanish - rudimentary in speaking; none in reading and writing.

Visiting Professorship(s)

2008 Visiting Scholar (1 weeks), Jilin University, China, Group of Nan Lu.

2007 Visiting Scholar (2 weeks), University of Toronto, Ontario, Canada, Group of Geofrey A. Ozin.

2007 Visiting Scholar (6 weeks), Northwestern University, Evanston, Illinois, Group of Chad A. Mirkin.

2006 Visiting Scholar (4 weeks), Northwestern University, Evanston, Illinois, Group of Chad A. Mirkin.

2005 Visiting Scholar (6 weeks), Northwestern University, Evanston, Illinois, Group of Chad A. Mirkin.

2004 Visiting Scholar (6 weeks), John Innes Center, UK, Group of Anne E. Osbourn.

2003 Visiting Scholar (4 weeks), Jilin University, China, Group of Wu Lixin.

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Honors, Awards, and Prizes

Nominee - Waterman Award, NSF (2013). Innovator Award, FSU Office of Researrch (2012). Nominee - The Pew Scholars Program in the Biomedical Sciences, The Pew charitable trusts (2012). Nominee - TR35 Technology Review magazine's innovator award, Technology Review magazine (2011).

Teaching

Courses Taught

Directed Individual Study (BSC5900) Graduate Tutorial in Biological Science (BSC5932) Nanotechnology (BSC5936) Nanotechnology (BSC4424) Biological Science I (BSC2010) General Biology for Nonmajors (BSC1005) Honors Work in Biological Science (BSC4970) Lipid Nanoscience (BSC5932) ROTATION III (BSC5900) Directed Individual Study (BSC4900) LIPID MICROARRAYS (BSC4900) Nanointaglio Antibiotic Screen (BSC4900) Directed Individual Study (CHM4905) NANOTECHNOLOGY (BSC4900) Nanotechnology (BSC4933) Selected Topics in Biological Science (BSC5936) Selected Topics in Biological Science (BSC4933) CELLULAR ALIGNMENT (BSC4900) Graduate Tutorials (BSC5932) PH BIOSENSING (BSC4900) PLANT MICROARRAYS (BSC4900) CELL CULTURE/MICROSCOPY (BSC4900) LAB AND PROPOSAL (BSC4900) LIPID BIOSENSING (BSC4900)

New Course Development

Nanotechnology (2015) Molecular modeling tutorials, Messiah College (1999)

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Doctoral Committee Chair

Vafai, N. R., graduate. (2018). Kusi-Appiah, A. E., graduate. (2016). Rubel Hoq, M., doctoral student.

Doctoral Committee Cochair

Lowry, T. W., graduate. (2016).

Doctoral Committee Member

Armstrong, R. E., graduate. (2015). Martinez, J. S., graduate. (2015). Wang, Z., graduate. (2015). Jia, D., graduate. (2015). Smithyman, J. R., graduate. (2014). Jevitt, A. M., doctoral candidate. Prommopan, P., doctoral candidate.

Doctoral Committee University Representative

Kountouriotis, K., graduate. (2018). Xia, J., graduate. (2016). Abou Shaheen, S. A., doctoral candidate. Ayad, S. A. H., doctoral candidate. Eaton, W. J., doctoral candidate. Goodman, K. E., doctoral candidate. Prommapan, P., doctoral candidate. Yarolimek, M. R., doctoral candidate. Campbell, A. T., doctoral student. Wei, I. A., doctoral student.

Master's Committee Member

Kay, D. I., graduate. (2017).

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Bachelor's Committee Member

Perez, M., student. [Honors thesis - FAMU-FSU College of Engineering] Gordon, J., student.

Additional Teaching Not Reported Elsewhere

Lenhert, S. (2013). Supervision of research rotation by Allison Jevitt.

Lenhert, S. (2013). Supervision of research rotation by Katherine Easterling.

Lenhert, S. (2013–2015). Supervision of undergraduate DIS for Alec Clark.

Lenhert, S. (2013–2014). Supervision of undergraduate DIS for Jacob Sanchez.

Lenhert, S. (2013–2015). Supervision of undergraduate DIS for Rainer Quinn.

Lenhert, S. J. (2012). Supervision of undergraduate DIS for Elham Shams. FSU.

Lenhert, S. J. (2011). Supervision of research rotation by Nicholas Vafai.

Lenhert, S. J. (2011). Supervision of undergraduate student Dorothy Jackson.

Lenhert, S. (2011). Faculty advisor for a peer reviewed publication in the FSU undergraduate research Journal: D. Jackson, S. Lenhert, A simple method for characterizing iridescence. Florida State Undergraduate Research Journal 1, 39-51 (2011).

Lenhert, S. J. (2011). Supervision of research rotation by Sunny Das.

Lenhert, S. J. (2011). Supervision of undergraduate "Nanotechnology Think Tank".

Lenhert, S. J. (2011). Supervision of undergraduate DIS for Sohail Merchant.

Lenhert, S. J. (2010). Supervision of undergraduate DIS for Caroline Black.

Lenhert, S. J. (2010). Supervision of research rotation by Ryan Riskowski. Institute for Molecular Biophysics.

Lenhert, S. J. (2010). Supervision of undergraduate DIS for Alyssa Teichen.

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Research and Original Creative Work

Program of Research and/or Focus of Original Creative Work

My field of research is nanobiotechnology, which involves the application of nanoscience and biology to solve technological problems. Nanoscience is a highly interdisciplinary field that seeks to understand how a material's properties change as its size is changed at length scales between the molecular scale and the macroscale. The integration of nanomaterials into functional devices is crucial for the development of technological applications of nanoscience, i.e. nanotechnology, and is an active area of my research. Biology provides an inspiration for nanoscience, with a living cell demonstrating some of the ultimate capabilities of miniaturization and integration of nanomaterials. Specifically, I am exploring a new sub-field of nanobiotechnology that I call lipid nanotechnology. This involves the fabrication, characterization and application of nano- and micro-structures composed of lipids. My research group creates these structures by advancing novel methods in nanofabrication including lipid dip-pen nanolithography and a new printing method that I've invented at FSU and named "nanointaglio." As a major biological function of lipids is compartmentalization, I seek to recreate that function in synthetic environments for applications in biosensing and high throughput screening.

Publications

Invited Journal Articles

Lenhert, S., & Vellanti, F. G. (2012). Drug discovery: a view through the looking glass. Future Medicinal Chemistry, 4(16), 2011-2013.

Refereed Journal Articles

Hamayun, M. A., Abramchuk, M., Alnasir, H., Khan, M., Pak, C., Lenhert, S., Ghazanfari, L., Shatruk, M., & Manzoor, S. (2018). Magnetic and magnetothermal studies of iron boride (FeB) nanoparticles. Journal of Magnetism and Magnetic Materials, 451, 407-413.

Kusi-Appiah, A. E., Mastronardi, M. L., Qian, C., Chen, K., Ghazanfari, L., Prommapan, P., Kübel, C., Ozin, G. A., & Lenhert, S. (2017). Enhanced cellular uptake of size-separated lipophilic silicon nanoparticles. Scientific Reports, 7, 1-9.

Lenhert, S., Kusi-Appiah, A. E., Lowry, T. W., Vafai, N., Van Winkle, D. H., & Lenhert, S. (2017). Fluid Lipid Multilayer Stabilization by Tetraethyl Orthosilicate for Underwater AFM Characterization and Cell Culture Applications. MRS Advances, 2, 3553-3558.

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Kumar, R., Urtizberea, A., Ghosh, S., Bog, U., Rainer, Q., Lenhert, S., Fuchs, H., & Hirtz, M. (2017). Polymer Pen Lithography with Lipids for Large-Area Gradient Patterns. Langmuir, 33, 8739–8748.

Lowry, T. W., Hariri, H., Prommapan, P., Kusi-Appiah, A., Lenhert, S., Vafai, N., Bienkiewicz, E. A., Winkle, D. V., Stagg, S. M., & Lenhert, S. (2016). Quantification of protein-induced membrane remodeling kinetics in vitro with lipid multilayer gratings. Small, 1-10.

Ghazanfari, L., & Lenhert, S. (2016). Screening of Lipid Composition for Scalable Fabrication of Solvent-Free Lipid Microarrays. Frontiers in Materials, 3, 1-9. doi:10.3389/fmats.2016.00055

Vafai, N., Lowry, T., Wilson, K., Davidson, M., & Lenhert, S. (2015). Evaporative edge lithography of a liposomal drug microarray for cell migration assays. Nanofabrication, 2, 32-42.

Tietz, S., Heather, M. E., Yarbrough, R., Semchonok, D., Lowry, T. W., Li, Z., Jahns, P., Boekema, E. J., Lenhert, S. J., Niyogi, K. K., & Kirchhoff, H. (2015). Functional implications of photosystem II crystal formation in photosynthetic membranes. Journal of Biological Chemistry, 290, 14091-14106.

Khosroshahi, M. E., Ghazanfari, L., Hassannejad, Z., & Lenhert, S. (2015). In-vitro application of doxorubicin loaded magnetoplasmonic thermosensitive liposomes for laser hyperthermia and chemotherapy of breast cancer. Journal of Nanomedicine & Nanotechnology, 6, 1-9.

Lowry, T. W., Prommapan, P., Rainer, Q., Van Winkle, D. H., & Lenhert, S. (2015). Lipid multilayer grating arrays integrated by nanointaglio for vapor sensing by an optical nose. Sensors, 15, 1-10.

Kusi-Appiah, A. E., Lowry, T. W., Darrow, E. M., Wilson, K., Chadwick, B. P., Davidson, M. W., & Lenhert, S. (2015). Quantitative dose-response curves from subcellular lipid multilayer microarrays. Lab on a Chip, 1-8.

Puthiyaveetil, S., Tsabari, O., Lowry, T., Lenhert, S., Lewis, R. R., Reich, Z., & Kirchhoff, H. (2014). Compartmentalization of the protein repair machinery in photosynthetic membranes. Proceedings of the National Academy of Sciences, 111, 15839-15844.

Lowry, T. W., Kusi-Appiah, A., Van Winkle, D. H., Davidson, D. W., & Lenhert, S. (2014). Materials integration by nanointaglio. Advanced Materials Interfaces, 1, 1300127 1-5.

Liu, Y., Kirkland, B., Shirley, J., Wang, Z., Zhang, P., Stembridge, J., Wong, W., Takebayashi, S.-i., Gilbert, D. M., Lenhert, S., & Guan, J. (2013). Development of single-cell array for large-scale DNA fluorescence in situ hybridization. Lab on a Chip, 13(7), 1316-1324.

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Kirkland, B., Wang, Z., Zhang, P., Takebayashi, S.-I., Lenhert, S., Gilbert, D. M., & Guan, J. (2013). Low-cost fabrication of centimetre-scale periodic arrays of single plasmid DNA molecules. Lab on a Chip, 13, 3367-3372.

Mitsakakis, K., Sekula-Neuner, S., Lenhert, S., Fuchs, H., & Gizeli, E. (2012). Convergence of dip-pen nanolithography and acoustic biosensors towards a rapid-analysis multi-sample microsystem. Analyst, 13(137), 3076-3082.

Kusi-Appiah, A. E., Vafai, N., Cranfill, P. J., Davidson, M. W., & Lenhert, S. (2012). Lipid multilayer microarrays for in vitro liposomal drug delivery and screening. Biomaterials, 33(16), 4187-4194.

Nafday, O. A., Lowry, T. W., & Lenhert, S. (2012). Multifunctional lipid multilayer stamping. Small, 8(7), 1021-1028.

Nafday, O. A., & Lenhert, S. (2011). High-throughput optical quality control of phospholipid multilayer fabrication by dip-pen nanolithography (DPN). Nanotechnology, 2, 1-7.

Tucker, S. L., Besi, M. I., Galhano, R., Franceschetti, M., Goetz, S., Lenhert, S., Osbourn, A., & Sesma, A. (2010). Common genetic pathways regulate organ-specific infection-related development in the rice blast fungus. Plant Cell, 22, 953¬972.

Schelb, M., Vannahme, C., Welle, A., Lenhert, S., Ross, B., & Mappes, T. (2010). Fluorescence excitation on monolithically integrated all-polymer chips. Journal of Biomedical Optics, 15, 041517.

Lenhert, S., Mirkin, C. A., & Fuchs, H. (2010). In situ lipid dip-pen nanolithography under water. Scanning, 31, 1–9.

Lenhert, S., Brinkmann, F., Laue, T., Walheim, S., Vannahme, C., Klinkhammer, S., Xu, M., Sekula, S., Mappes, T., Schimmel, T., & Fuchs, H. (2010). Lipid multilayer gratings. Nanotechnology, 5, 275–279.

Wang, Y., Giam, L. R., Park, M., Lenhert, S., Fuchs, H., & Mirkin, C. A. (2008). A self-correcting inking strategy for cantilever arrays addressed by an inkjet printer and used for dip-pen nanolithography. Small, 4, 1666–1670.

Sekula, S., Fuchs, J., Weg-Remers, S., Nagel, P., Schuppler, S., Fragala, J., Theilacker, N., Franzreb, M., Wingren, C., Ellmark, P., Borrebaeck, C. A. K., Mirkin, C. A., Fuchs, H., & Lenhert, S. (2008). Multiplexed lipid dip-pen nanolithography on subcellular scales for templating of functional proteins and cell culture. Small, 4, 1785–1793.

Kirchhoff, H., Lenhert, S., Buchel, C., Chi, L., & Nield, J. (2008). Probing the organization of photosystem II in photosynthetic membranes by atomic force microscopy. Biochemistry, 47, 431–440.

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Lenhert, S., Sesma, A., Hirtz, M., Chi, L., Fuchs, H., Wiesmann, H. P., Osbourn, A. E., & Moerschbacher, B. M. (2007). Capillary-induced contact guidance. Langmuir, 23, 10216–10223.

Plutowski, U., Jester, S. S., Lenhert, S., Kappes, M. M., & Richert, C. (2007). DNA-based self-sorting of nanoparticles on gold surfaces. Advanced Materials, 19, 1951–1956.

Chen, X. D., Lenhert, S. J., Hirtz, M., Lu, N., Fuchs, H., & Chi, L. F. (2007). Langmuir-Blodgett patterning: A bottom-up way to build mesostructures over large areas. Accounts of Chemical Research, 40, 393-401.

Lenhert, S., Sun, P., Wang, Y. H., Fuchs, H., & Mirkin, C. A. (2007). Massively parallel dip-pen nanolithography of heterogeneous supported phospholipid multilayer patterns. Small, 3, 71–75.

Wu, X. C., Lenhert, S., Chi, L. F., & Fuchs, H. (2006). Interface interaction controlled transport of CdTe nanoparticles in the microcontact printing process. Langmuir, 22, 7807–7811.

Lenhert, S., Gleiche, M., Fuchs, H., & Chi, L. F. (2005). Mechanism of regular pattern formation in reactive dewetting. Chemphyschem, 6, 2495–2498.

Lenhert, S., Meier, M. B., Meyer, U., Chi, L. F., & Wiesmann, H. P. (2005). Osteoblast alignment, elongation and migration on grooved polystyrene surfaces patterned by Langmuir-Blodgett lithography. Biomaterials, 26, 563–570.

Zhang, M. Z., Lenhert, S., Wang, M., Chi, L. F., Lu, N., Fuchs, H., & Ming, N. B. (2004). Regular arrays of copper wires formed by template-assisted electrodeposition. Advanced Materials, 16, 409–413.

Lenhert, S., Zhang, L., Mueller, J., Wiesmann, H. P., Erker, G., Fuchs, H., & Chi, L. F. (2004). Self-organized complex patterning: Langmuir-Blodgett lithography. Advanced Materials, 16, 619–624.

Kirchhoff, H., Borinski, M., Lenhert, S., Chi, L. F., & Buchel, C. (2004). Transversal and lateral exciton energy transfer in grana thylakoids of spinach. Biochemistry, 43, 14508–14516.

Lu, N., Gleiche, M., Zheng, J. W., Lenhert, S., Xu, B., Chi, L. F., & Fuchs, H. (2002). Fabrication of chemically patterned surfaces based on template-directed self-assembly. Advanced Materials, 14, 1812–1815.

Gao, S., Chi, L., Lenhert, S., Anczykowski, B., Niemeyer, C. M., Adler, M., & Fuchs, H. (2001). High-quality mapping of DNA-protein complexes by dynamic scanning force microscopy. Chemphyschem, 2, 384–388.

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Niemeyer, C. M., Adler, M., Lenhert, S., Gao, S., Fuchs, H., & Chi, L. F. (2001). Nucleic acid supercoiling as a means for ionic switching of DNA-nanoparticle networks. Chembiochem, 2, 260–264.

Niemeyer, C. M., Adler, M., Pignataro, B., Lenhert, S., Gao, S., Chi, L. F., Fuchs, H., & Blohm, D. (1999). Self-assembly of DNA-streptavidin nanostructures and their use as reagents in immuno-PCR. Nucleic Acids Research, 27, 4553–4561.

Invited Book Chapters

Lenhert, S. (2010). Introduction to nanobiology. In Introduction to Nanoscale Science and Technology (pp. 163–209). NanoInk, Inc.

Lenhert, S., Fuchs, H., & Mirkin, C. A. (2009). Materials integration by dip-pen nanolithography. In Fuchs, H. (Ed.), Nanoprobes Vol. 6 Nanotechnology (pp. 171–196). Weinheim: Wiley-VCH.

Lenhert, S. J. (2004). Heterogeneous surfaces with nanosized channel lattices. In James A. Schwarz, Christian Contescu, & Karol Putyera (Eds.), Dekker Encyclopedia of Nanoscience and Nanotechnology. New York, Marcel Dekker.

Nonrefereed Books

Lenhert, S. (2011). BSC 1005 Synthetic Biology. Hayden-McNeil.

Nonrefereed Reports

Lenhert, S., & Brinkmann, F. (2010). Optische Biosensoren aus Lipid-Gitter. German Language.

Presentations

Refereed Papers at Conferences

Lenhert, S. (presented 2014, December). Lipid Multilayer Nanostructures on Surfaces As Bioinspired Compartments. Paper presented at EMBS Micro and Nanotechnology in Medicine Conference, IEEE Engineering in Medicine & Biology Society, Oahu, HI. (International) Retrieved from http://mnm.embs.org/2014/

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Invited Presentations at Conferences

Lenhert, S. (accepted). Lipid multilayer grating based biosensors. Presentation to be given at 4th International Symposium "Lasers in Medicine and Biophotonics", Center of Laser Technology and Material Science, Russan Federation, St. Petersburg, Russia. (International)

Lenhert, S. J. (presented 2019, November). Lipid Droplet Microarrays. Presentation at NanoFlorida 2019 International Conference, Florida Association for Nanotechnology. (International)

Lenhert, S. (presented 2018, November). Miniaturizing the test tube with lipid nanotechnology. Presentation at Nanobio Collaborative International Conference: "Advances in Pharmaceutical Nanotechnology", USF and FAMU, Tampa. (International)

Lenhert, S. (presented 2014). Lipid nanotechnologies for drug screening and biosensor microarrays. Presentation at Energy, Materials, and Nanotechnology (EMN), OAHost, Orlando. (International)

Lenhert, S. J. (presented 2013). Surface supported lipid nanotechnology. Presentation at 7th International Conference on Materials for Advanced Technologies (ICMAT), Materials Research Society of Singapore (MRS-S), Singapore. (International)

Lenhert, S. (presented 2011). Functional lipid nanostructuresassembled by dip-pen nanolithography. Presentation at 6th International Conference on Materials for Advanced Technologies (ICMAT), Materials Research Society of Singapore (MRS-S), Singapore. (International)

Lenhert, S. J. (presented 2011). Lipid multilayer micro and nanoarrays for delivery of materials to cells. Presentation at 6th International Conference on Materials for Advanced Technologies, Materials Research Society of Singapore (MRS-S), Singapore. (International)

Lenhert, S. (presented 2011). Nanostructured lipid multilayer fabrication by dip-pen nanolithography and multilayer stamping. Presentation at 2011 MRS Fall Meeting and Exhibit, MRS, Boston, Massachusetts. (International)

Lenhert, S. (presented 2010). Functional lipid nanostructures fabricated by dip-pen nanolithography. Presentation at Tech Connect World Conference and Expo, Tech Connect World, Anaheim, California. (International)

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Invited Presentations at Symposia

Lenhert, S. J. (presented 2013, July). Lipid Nanotechnologies for Sensing and Drug Discovery. In Martin Wegener (Chair), 12 years of CFN. Presentation at the meeting of Center for Functional Nanomaterials (CFN), Karlsruhe Germany. (International)

Refereed Presentations at Conferences

Lenhert, S. J. (presented 2019, December). Lipid Droplet Microarrays. Presentation at MRS Fall Meeting, Materials Research Society, Boston. (International)

Liu, S., Feng, K., Bell, T., Van Winkle, D., & Lenhert, S. J. (presented 2019, November). Diffusion of dye across a two-phase boundary in lipid droplet microarrays. Poster presentation at NanoFlorida 2019 International Conference, Florida Association for Nanotechnology. (International)

Bell, T., Feng, K., Calvin, G., Liu, S., Van Winkle, D., & Lenhert, S. J. (presented 2019, November). Dye partitioning kinetics in lipid droplet microarrays. Poster presentation at NanoFlorida 2019 International Conference, Florida Association for Nanotechnology. (International)

Kusi-Appiah Aubrey, & Lenhert, S. J. (presented 2019, November). Lipid droplet microarrays for lipophilic small molecule screening. Poster presentation at NanoFlorida 2019 International Conference, Florida Association for Nanotechnology. (International)

Feng, K., Bell, T., Liu, S., Calvin, G., Van Winkle, D., & Lenhert, S. J. (presented 2019, November). Selective partitioning of small molecules in lipid droplet microarrays. Poster presentation at NanoFlorida 2019 International Conference, Florida Association for Nanotechnology. (International)

Lenhert, S. (presented 2018, December). Lipid droplet microarrays for high throughput screening in synthetic biology. Presentation at Materials Research Society Fall Meeting, Materials Research Society, Boston. (International)

Lenhert, S. (presented 2017, April). Lipid Multilayer Grating Arrays as Label-Free Cytomimetic Aqueous Sensors. Presentation at 2017 MRS Spring Meeting & Exhibit, Materials Research Society (MRS). (International)

Lenhert, S. (presented 2017, April). Scalable Materials Integration into Lipid Multilayer Microarrays for Cell-Based High-Throughput Screening. Presentation at 2017 MRS Spring Meeting & Exhibit, Materials Research Society (MRS). (International)

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Lenhert, S. (presented 2017, February). Protein induced membrane remodeling assayed in vitro using lipid multilayer micro- and nanostructres. Poster presentation at 61st Annual Meeting of the Biophysical Society, Biophysical Society, New Orleans. (International)

Lenhert, S. (presented 2016, November). Lipid Microarray Technology (LiMiT), a Miniaturized Toxicity Screening System. Presentation at Techconnect, Defense Innovation Technology Acceleration Challenges. (International)

Lenhert, S. (presented 2014). Integration of lipid multilayer gratings into multimaterial biosensor arrays. Presentation at 2014 MRS Fall Meeting & Exhibit, Materials Research Society (MRS), Boston, MA. (International)

Lenhert, S. (presented 2014). Surface supported lipid nanotechnology. Poster presentation at Annual Meeting of the Biophysical Society, Biophysical Society, San Francisco. (International)

Lenhert, S. J. (presented 2012). Liposome microarrays for in vitro drug delivery and screening. Presentation at Colloids and Nanomedicine, Elsevier, Amsterdam, the Netherlands. (International)

Biswas, S., Hirtz, M., Lenhert, S., & Fuchs, H. (presented 2010). Measurement of DPN-ink viscosity using an AFM cantilever. Presentation at Tech Connect World Conference and Expo, Tech Connect World, Anaheim, California. (International)

Nafday, O., & Lenhert, S. (presented 2010). Optical quality control of lipid nanostructured created via dip-pen nanolithography. Presentation at Gordon Research Conference on Nanostructure Fabrication, Gordon Research Conferences, Tilton, NH. (National)

Refereed Presentations at Symposia

Lenhert, S. (presented 2014). Lipid Multilayer Gratings as Label Free Biosensor Elements. In 11th Annual International Workshop on Nanomechanical Sensing. Presentation at the meeting of NMC, Madrid, Spain. (International)

Lenhert, S. (presented 2013). Lipid Multilayer Gratings as Label Free Sensing Elements. In NMC 2013. Poster presentation at the meeting of NMC, Stanford, CA. (International)

Nonrefereed Presentations at Symposia

Lenhert, S. (presented 2010, December). Lipid multilayer arrays as model cellular systems. In Fowler Symposium, Frontiers in Molecular Biology. Presentation at the meeting of Department of Biological Science, Florida State University, Tallahassee, Florida. (Local)

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Lenhert, S. (presented 2010, April). Toward functional lipid-based biometamaterials. In Wyss Symposium: New Directions in Synthetic Biology. Presentation at the meeting of Harvard Medical School, Boston, Massachusetts. (National)

Invited Lectures and Readings of Original Work

Lenhert, S. (2015, April). Lithography. Delivered at FSU. (Local)

Lenhert, S. (2015, February). Lipid nanotechnology. Delivered at FSU. (Local)

Lenhert, S. (2014, November). Towards natural product libraries on a chip. Delivered at FSU, Department of Biological Sciences, Plant Sciences Seminar, Tallahassee, FL. (Local)

Lenhert, S. (2014, September). Lipid multilayer nanostructures on surfaces as bioinspired compartments. Delivered at FSU, College of Engineering, Biomedical Engineering Seminar, Tallahassee, Florida. (Local)

Lenhert, S. (2014, April). Lithography. Delivered at FSU. (Local)

Lenhert, S. J. (2013, January). Lipid nanotechnologies for diagnostic sensor arrays and high throughput drug screening microarrays. Delivered at FSU Biomedical Sciences Seminar, FSU - College of Medicine. (Local)

Lenhert, S. J. (2012, December). Surface supported lipid nanotechnology. Delivered at Rutgers University, New Jersey, USA. (Local)

Lenhert, S. J. (2012, July). Surface supported lipid nanotechnology. Delivered at University of Muenster, Muenster, Germany. (International)

Lenhert, S. J. (2012, May). Liposome microarrays for in vitro drug delivery and screening. Delivered at FSU - Integrative NanoScience Institute, internal meeting, Tallahassee, FL. (Local)

Lenhert, S. J. (2011, December). Surface Supported Lipid Nanotechnology. Delivered at FSU - presentation at the internal INSI meeting, Tallahassee, FL. (Local)

Lenhert, S. J. (2011, August). Surface supported lipid nanotechnology. Delivered at Northwestern University, Evanston Illinois. (International)

Lenhert, S. (2011, April). Biomedical applications of nanoanalysis and nanofabrication. Delivered at FSU, Tallahassee, Florida. (Local)

Lenhert, S. (2011, March). Lipid nanodynamics - towards multi-functional biomimetic membrane systems. Delivered at Emory University, Atlanta, Georgia. (Local)

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Lenhert, S. (2011, March). Nanotechnology as a route towards synthetic biology. Delivered at FSU Faculty Luncheon Series, Tallahassee, Florida. (Local)

Lenhert, S. J. (2011, February). Bio-nanotechnology. Delivered at FSU - Frontiers in Biology Seminar, Tallahassee, FL. (Local)

Lenhert, S. J. (2011, February). Functional lipid nanostructures assembled by dip-pen nanolithography. Delivered at University of Alabama at Birmingham, Birmingham Alabama. (Local)

Lenhert, S. J. (2011, February). Structure, color and function in biology and nanotechnology. Delivered at FSU, Tallahassee, FL. (Local)

Lenhert, S. (2011, January). Functional lipid nanostructures assembled by dip-pen nanolithography. Delivered at Lawrence Berkeley National Lab, Berkeley, California. (Local)

Lenhert, S. (2011, January). Functional lipid nanostructures assembled by dip-pen nanolithography. Delivered at University of Nevada, Las Vegas, Nevada. (Local)

Lenhert, S. (2010, November). Functional lipid biomaterials fabricated by dip-pen nanolithography. Delivered at FSU Materials Science and Engineering Course, Tallahassee, Florida. (Local)

Lenhert, S. J. (2010, October). Lipid nanodynamics - towards functional biomimetic membrane systems. Delivered at FSU - Molecular Biophysics Seminar, Tallahassee, FL. (Local)

Lenhert, S. J. (2010, August). Tips for teaching. Delivered at FSU, Tallahassee, FL. (Local)

Lenhert, S. (2010, May). Functional lipid nanostructures assembled by dip-pen nanolithography. Delivered at Ecole Normale Supérieur, Paris, France. (International)

Lenhert, S. (2010, March). Cell adhesion investigated with structured surfaces, model systems and capillary theory. Delivered at FSU Compustat Seminar Series, Tallahassee, Florida. (Local)

Lenhert, S. (2010, March). Functional lipid nanostructures fabricated by dip-pen nanolithography. Delivered at FSU Chemistry and Biochemistry, Tallahassee, Florida. (Local)

Lenhert, S. (2010, February). Functional lipid nanostructures fabricated by dip-pen nanolithography. Delivered at FSU Chemical and Biomedical Engineering, Tallahassee, Florida. (Local)

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Patented Inventions

Lenhert, S., & Kusi-Appiah, A. (submitted). Surface Treated Lipid Supported Multilayers.

Lenhert, S. J., Nafday, O., & Lowry, T. W. (submitted). Methods and Apparatus for Lipid Muitilayer Patterning. Patent pending, 13/417,588, FSU.

Lenhert, S. J., & Nafday, O. A. (submitted). Optical method for measuring height of fluorescent phospholipid features fabricated via dip-pen nanolithography. Patent pending, US 13/234,540.

Lenhert, S. J., & Vafai, N. (2018). Evaporative edge lithography of a liposomal drug microarray for cell migration assays. US 9,995,732.

Lenhert, S. (2018). Methods and devices for modulating the tempo of music in real time based on physiological rhythms. US 10,152,957.

Lenhert, S., & Kusi-Appiah, A. (2016). Lipid multilayer microarrays and their use for cell culture screening. US 9.447,446, FSU.

Lenhert, S. J., Lowry, T. W., & Kusi-Appiah, A. (2016). Scalable liposome microarray screening. US 9,513,222.

Lenhert, S. J. (2015). Semi-synthetic quorum sensors. US 9,102,974.

Lenhert, S. J. (2013). Method for demonstrating the presence of molecules by means of optical gratings. Karlsruhe Institute of Technology. Germany.

Lenhert, S., & Fuchs, H. (2012). Method to apply membrane-lipids to a substrate. Karlsruhe Institute of Technology. Germany.

Contracts and Grants

Contracts and Grants Funded

Lenhert, Steven John (PI). (Aug 2017–May 2019). Lipid Enhanced Nano-Sensors (LENS) for Lead and Detection in Water. Funded by Zansors, LLC. (N/A). Total award $79,999.

Lenhert, Steven John (PI). (Jun 2016–May 2019). High-Throughput Lipophilic Drug Screening Microarrays. Funded by National Institute of General Medical Sciences. (R01GM107172). Total award $550,002.

Balicas, Luis Molinuevo (Co-PI), Xin, Yan (PI), Liang, Zhiyong (Co-PI), Kametani Fumitake (Co-PI), Shatruk, Mykhailo (Co-PI), Lenhert, Steven John (Co-PI), Ma, Biwu (Co-PI), &

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Gao, Hanwei (Co-PI). (May 2016–May 2017). EIEG: A Tool for Improving Data Acquisition in FSU's Sub-Angstrom Resolution TEM by Reducing Surface Contamination of Samples. Funded by FSU CRC. Total award $21,500.

Locke, Bruce R (Co-PI), Steinbock, Oliver (Co-PI), Zheng, Jianping (Co-PI), Alabugin, Igor V (Co-PI), Roper, Michael Gabriel (Co-PI), Oates, William (Co-PI), Liu, Tao (PI), Guan, Jingjiao (Co-PI), Lenhert, Steven John (Co-PI), Hallinan, Daniel (Co-PI), & Ma, Biwu (Co-PI). (Jun 2014–May 2015). EIEG: Accquisition of a High Speed Camera with Microscope. Funded by FSU EIEG Award. Total award $52,335.

Lenhert, Steven John (PI). (Sep 2013–May 2016). High Throughput Lipophilic Drug Screening Microarrays. Funded by National Institutes of Health. (1R01GM107172). Total award $750,749.

Von Molnar, Stephan (Co-PI), Lochner, Eric J (Co-PI), Guan, Jingjiao (Co-PI), & Lenhert, Steven John (PI). (Nov 2011–Nov 2012). EIEG: Critical Enhancement of INSI/CMMP AFM Facility. Funded by FSU EIEG Award. Total award $50,000.

Lenhert, Steven John (PI). (Nov 2011–Nov 2012). FSU Research Foundation - GAP. Funded by FSU GAP I. (INITIAL). Total award $15,000.

Lenhert, Steven John (PI). (Aug 2010–Jul 2016). Lipid Dip-Pen Nanolithography. Funded by Karlsruher Institute for Technologies. Total award $22,500.

Contracts and Grants Pending

Lenhert, Steven John (PI). (Sep 2018). Lipid microarray technology (LiMiT): miniaturized screening of anticancer drugs for precision medicine. Submitted to Lipid Nanotech, LLC.

Lenhert, Steven John (PI). (Mar 2018). High throughput screening with lipid droplet microarrays. Submitted to National Institute of General Medical Sciences.

Lenhert, S. (Jan 2018). Lipid microarray technology (LiMiT): miniaturized screening of anticancer drugs for precision medicine. Submitted to Lipid Nanotech, LLC (subcontract from NIH SBIR).

Lenhert, Steven John (PI). (Nov 2017). SemiSynBio:Collaborative proposal: Nucleus on a chip - a lipid microarray based DNA memory. Submitted to Florida International University.

Lenhert, Steven John (PI). (May 2017). Miniaturizing the test tube with lipid nanotechnology. Submitted to National Institutes of Health.

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Postdoctoral Supervision

Kusi-Appiah, A. E. (2016–present).

Lowry, T. W. (2016–present).

Bresch, S. (2016–17).

Ghazanfari, L. (2014–16).

Errahali, Y. (2013–14).

Tietz, S. (2013).

Nafday, O. (2010–12).

Service

Florida State University

FSU University Service

Member, Biomedical Engineering; Biomolecular Science Committee, Interdiciplinary Research and Commercialization Building planning (2015–2016).

Member, Materials & Energy Search Committee (2013–2015).

Senator, Faculty Senate (2012–2014).

Member, Academic honor policy hearing panel (2010).

FSU College Service

Member, Life Sciences Symposium Committee (2012–2014).

FSU Department Service

Chairperson, First Year Advisory Commitee (2019–2020).

Representative, CMB Area Representative (2019–2020).

Instructor, Young Scolar's Program (2019).

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Member, Faculty Evaluation Committee (2016).

Member, Department of Biological Science Elections Committee (2011–2012).

FSU Institute or Center Service member, Interdisciplinary Research and Commercialization Building (IRCB) planning committee (2015–present). member, Materials Science and Engineering Academic Advisory Committee (2011–2017). member, Steering Committee for the FSU Integrative NanoScience Institute (2010–2013). member, Steering Committee for FSU Materials Website (2010–2012).

FSU Program Service

Member, Recruitment committee for Cell and Molecular Biology (2015–2016).

The Profession

Editorial Board Membership(s)

Nanofabrication (2012–present).

Materials Today (2010–present).

Guest Reviewer for Refereed Journals

ACS Applied Materials & Interfaces (2014–present).

Beilstein Journal of Nanotechnology (2013–present).

Chemical Science (2013–present).

Journal of Biomedical Materials Research: Part A (2013–present).

RSC Advances (2013–present).

Tetrahedron (2013–present).

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The Journal of Physical Chemistry (2013–present).

ACS Nano (2012–present).

Advanced Healthcare Materials (2012–present).

Journal of Biological Engineering (2012–present).

Journal of the American Chemical Society (JACS) (2012–present).

Journal of Biomedicine and Biotechnology (2011–present).

Jove (2011–present).

Advanced Materials (2010–present).

Angewandte Chemie (2010–present).

Biomaterials (2010–present).

Langmuir (2010–present).

Scanning (2010–present).

Small (2010–present).

Soft Matter (2010–present).

Reviewer or Panelist for Grant Applications

Chilean Government (CONICYT) (2013).

National Science Foundation (NSF) (2012).

German Research Foundation (DFG) (2012).

National Science Foundation (NSF) (2011).

Service to Other Universities

Promotion and tenure letter writer, The Keck Science Department of Claremont McKenna, Scripps, and Pitzer Colleges (2018).

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Consultation

Nanoink, Inc. Advisory board member for Nanoprofessor project (2009–2012).

CMP Cientifica, Spain. Contributions to The Nanotechnology Opportunity Report (2001).

About.com, Inc. Nanotechnology guide (1999–2001).

Lux Capital. Contributions to The Nanotechnology Report (2000).

Additional Service Not Reported Elsewhere

Lenhert, S. J. (2011). Associate Faculty in Materials Science and Engineering. FSU.

Lenhert, S. J. (2011). Associate Faculty in the Institute for Molecular Biophysics.

Lenhert, S. (2010). Writing Recommendation Letters for Students and postdocs, and letters of support for Colleagues.

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