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												  TLC–Densitometry Analysis of Indole Compounds in Mycelial Culture of Imleria Badia and Agaricus Bisporus Enriched with Precursors — Serine Or Anthranilic AcidOriginal Research Paper TLC–Densitometry Analysis of Indole Compounds in Mycelial Culture of Imleria badia and Agaricus bisporus Enriched with Precursors — Serine or Anthranilic Acid Włodzimierz Opoka1*, Katarzyna Kała2, Remigiusz Krężałek2, Katarzyna Sułkowska-Ziaja2, Anna Maślanka1 and Bożena Muszyńska2 1Department of Inorganic Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, Kraków, Poland 2Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Kraków, Poland Received: 14 May 2017; accepted: 05 June 2017 Agaricus bisporus and Imleria in vitro cultures were cultivated on modified Oddoux medium, and Oddoux medium was enriched with serine or anthranilic acid. Serine or anthranilic acid was used at the concentrations of 0.1, 0.25, 0.5, and 0.75 g/L of medium. Determination of indole compounds in the obtained biomass was carried out using thin-layer chromatography (TLC) with densitometric detection. In every analyzed sample, presence of serine or anthranilic acid was studied. Comparison of the results obtained for the treatment and control samples allowed us to determine the optimum concentration of serine or anthranilic acid in the medium in order to obtain biomass with increased content of indole compounds. A. bisporus with addition of anthranilic acid or serine to the medium at the concentration of 0.5 g/L was the most beneficial. In the case of Imleria badia, anthranilic acid at the concentration of 0.5 g/L was the most optimal. This is the first report demonstrating the content of indole derivatives in biomass affected by their precursors (serine or anthranilic acid). The study indicates that modification of the medium can provide satisfactory results, and it is worth to search for its new, improved compositions.
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												  Boletus Mushrooms La Tia Jackson, Ian CGenetic Diversity within Alaskan Boletus Mushrooms www.fungi-zette.com La Tia Jackson, Ian C. Herriott, József Geml, Gary A. Laursen, D. Lee Taylor Discussion Abstract •Clade 1 is composed of Alaskan samples from the interior and Northwest and We analyzed the genetic differences within the collection of samples from Europe. Although the species names do not match up they are genetically very similar, and B. citrinovirens is thought to be in what is called the Boletus mushroom from the UAF Fungal Herbarium, representing Identification on Tree Location subtomentosus group. samples from all over Alaska. Upon analyzing the DNA sequence Results DQ066405_Boletus_citrinovirens Europe from 18 samples, we found that most Alaskan Boletus are closely DQ066407_Boletus_spadiceus Europe •Clade 2 is composed of Alaskan samples from Southeast Alaska and GenBank DQ066397_Boletus_citrinovirens Europe samples from Europe, both identified as Boletus mirabilis. related to samples from the same species found in other parts of the world (clades 1,2,5,6,8,12). We also found that some species DQ066410_Boletus_spadiceus Europe •Clade 3 is composed of samples only from Alaska. None of the sequences DQ384578_Boletellus_mirabilis Europe collected in other parts of the world were not found in our sample 1 mycorance.free.fr obtained from GenBank are closely related. These were morphologically collection (clades 4,7,9,10,11). Finally, our results suggested that AJ419187_Boletus_impolitus Spain identified as B. subglabripes. “B. subglabripes” is in GenBank, but only Large 100 DQ131632_Xerocomus_subtomentosus Europe clade 3 on our phylogenetic tree is not represented among the Subunit gene, not Internally Transcribed Spacer gene (reference 2) so comparison AJ889931_Boletus_pruinatus Europe GenBank sequences from other parts of the world and may be a 100 is not possible to determine if morphological species attribution is the same or 2 AM087271_Xerocomus_pruinatus Europe genetic lineage endemic to Alaska.
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												  Influence of Tree Species on Richness and Diversity of Epigeous FungalView metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Archive Ouverte en Sciences de l'Information et de la Communication fungal ecology 4 (2011) 22e31 available at www.sciencedirect.com journal homepage: www.elsevier.com/locate/funeco Influence of tree species on richness and diversity of epigeous fungal communities in a French temperate forest stand Marc BUE´Ea,*, Jean-Paul MAURICEb, Bernd ZELLERc, Sitraka ANDRIANARISOAc, Jacques RANGERc,Re´gis COURTECUISSEd, Benoıˆt MARC¸AISa, Franc¸ois LE TACONa aINRA Nancy, UMR INRA/UHP 1136 Interactions Arbres/Microorganismes, 54280 Champenoux, France bGroupe Mycologique Vosgien, 18 bis, place des Cordeliers, 88300 Neufchaˆteau, France cINRA Nancy, UR 1138 Bioge´ochimie des Ecosyste`mes Forestiers, 54280 Champenoux, France dUniversite´ de Lille, Faculte´ de Pharmacie, F59006 Lille, France article info abstract Article history: Epigeous saprotrophic and ectomycorrhizal (ECM) fungal sporocarps were assessed during Received 30 September 2009 7 yr in a French temperate experimental forest site with six 30-year-old mono-specific Revision received 10 May 2010 plantations (four coniferous and two hardwood plantations) and one 150-year-old native Accepted 21 July 2010 mixed deciduous forest. A total of 331 fungal species were identified. Half of the fungal Available online 6 October 2010 species were ECM, but this proportion varied slightly by forest composition. The replace- Corresponding editor: Anne Pringle ment of the native forest by mono-specific plantations, including native species such as beech and oak, considerably altered the diversity of epigeous ECM and saprotrophic fungi. Keywords: Among the six mono-specific stands, fungal diversity was the highest in Nordmann fir and Conifer plantation Norway spruce plantations and the lowest in Corsican pine and Douglas fir plantations.
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												  CZECH MYCOLOGY Publication of the Czech Scientific Society for MycologyCZECH MYCOLOGY Publication of the Czech Scientific Society for Mycology Volume 57 August 2005 Number 1-2 Central European genera of the Boletaceae and Suillaceae, with notes on their anatomical characters Jo s e f Š u t a r a Prosetická 239, 415 01 Tbplice, Czech Republic Šutara J. (2005): Central European genera of the Boletaceae and Suillaceae, with notes on their anatomical characters. - Czech Mycol. 57: 1-50. A taxonomic survey of Central European genera of the families Boletaceae and Suillaceae with tubular hymenophores, including the lamellate Phylloporus, is presented. Questions concerning the delimitation of the bolete genera are discussed. Descriptions and keys to the families and genera are based predominantly on anatomical characters of the carpophores. Attention is also paid to peripheral layers of stipe tissue, whose anatomical structure has not been sufficiently studied. The study of these layers, above all of the caulohymenium and the lateral stipe stratum, can provide information important for a better understanding of relationships between taxonomic groups in these families. The presence (or absence) of the caulohymenium with spore-bearing caulobasidia on the stipe surface is here considered as a significant ge neric character of boletes. A new combination, Pseudoboletus astraeicola (Imazeki) Šutara, is proposed. Key words: Boletaceae, Suillaceae, generic taxonomy, anatomical characters. Šutara J. (2005): Středoevropské rody čeledí Boletaceae a Suillaceae, s poznámka mi k jejich anatomickým znakům. - Czech Mycol. 57: 1-50. Je předložen taxonomický přehled středoevropských rodů čeledí Boletaceae a. SuiUaceae s rourko- vitým hymenoforem, včetně rodu Phylloporus s lupeny. Jsou diskutovány otázky týkající se vymezení hřibovitých rodů. Popisy a klíče k čeledím a rodům jsou založeny převážně na anatomických znacích plodnic.
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												  Comment on “Study of Biological Activity of Tricholoma Equestre Fruiting Bodies and Their Safety for Human”European Food Research and Technology https://doi.org/10.1007/s00217-019-03236-w SHORT COMMUNICATION Comment on “Study of biological activity of Tricholoma equestre fruiting bodies and their safety for human” Piotr Rzymski1 · Piotr Klimaszyk2 · Denis Benjamin3 Received: 18 December 2018 / Accepted: 3 January 2019 © The Author(s) 2019 Keywords Mushrooms · Toxicity · Tricholoma equestre Dear Editor, countries (e.g., France, Spain, and Italy) to officially declare T. equestre as poisonous, releasing warnings to avoid its con- The paper by Muszyńska et al. [1] entitled “Study of biologi- sumption. Prior to this, it had a long -history of consumption cal activity of Tricholoma equestre fruiting bodies and their in many countries with no anecdotal or scientific evidence safety for human” published in the journal of European Food of toxic effects. It is still considered edible and widely con- Research and Technology (https ://doi.org/10.1007/s0021 sumed in regions such as Poland [8]. As shown in a recent 7-018-3134-0) presents the results of a study on the compo- study, more than half of mushroom foragers in this country sition of T. equestre (syn. T. flavovirens, and syn. T. auratum) have consumed T. equestre at least once in their lifetime [9]. and in vitro anti-microbial and antioxidant activities of this This questionnaire survey and additional analysis of Polish mushroom. As reported, T. equestre can be a source of some registry of mushroom toxicity demonstrate that no rhabdo- essential minerals and has a favorable ratio of unsaturated- myolysis was reported in the last decade and that only mild to-saturated fatty acids, while its extracts exhibit antioxidant gastrointestinal effects were noted, at a lower frequency than effects (as shown in DPPH assay) at levels comparable to for other well-established edible wild mushrooms, such as other mushroom species, but weak anti-microbial activities.
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												  Xerocomus S. L. in the Light of the Present State of KnowledgeCZECH MYCOL. 60(1): 29–62, 2008 Xerocomus s. l. in the light of the present state of knowledge JOSEF ŠUTARA Prosetická 239, 415 01 Teplice, Czech Republic [email protected] Šutara J. (2008): Xerocomus s. l. in the light of the present state of knowledge. – Czech Mycol. 60(1): 29–62. The definition of the generic limits of Xerocomus s. l. and particularly the delimitation of this genus from Boletus is very unclear and controversial. During his study of European species of the Boletaceae, the author has come to the conclusion that Xerocomus in a wide concept is a heterogeneous mixture of several groups of species. These groups are separated from each other by different anatomical and some other characters. Also recent molecular studies show that Xerocomus s. l. is not a monophyletic group. In agreement with these facts, the European species of Xerocomus s. l. whose anatomy was studied by the present author are here classified into the following, more distinctly delimited genera: Xerocomus s. str., Phylloporus, Xerocomellus gen. nov., Hemileccinum gen. nov. and Pseudoboletus. Boletus badius and Boletus moravicus, also often treated as species of Xerocomus, are retained for the present in the genus Boletus. The differences between Xerocomus s. str., Phylloporus, Xerocomellus, Hemileccinum, Pseudoboletus and Boletus (which is related to this group of genera) are discussed in detail. Two new genera, Xerocomellus and Hemileccinum, and necessary new combinations of species names are proposed. Key words: Boletaceae, Xerocomus, Xerocomellus, Hemileccinum, generic taxonomy, anatomy, histology. Šutara J. (2008): Rod Xerocomus s. l. ve světle současného stavu znalostí. – Czech Mycol.
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												  AR TICLE New Sequestrate Fungi from Guyana: Jimtrappea GuyanensisIMA FUNGUS · 6(2): 297–317 (2015) doi:10.5598/imafungus.2015.06.02.03 New sequestrate fungi from Guyana: Jimtrappea guyanensis gen. sp. nov., ARTICLE Castellanea pakaraimophila gen. sp. nov., and Costatisporus cyanescens gen. sp. nov. (Boletaceae, Boletales) Matthew E. Smith1, Kevin R. Amses2, Todd F. Elliott3, Keisuke Obase1, M. Catherine Aime4, and Terry W. Henkel2 1Department of Plant Pathology, University of Florida, Gainesville, FL 32611, USA 2Department of Biological Sciences, Humboldt State University, Arcata, CA 95521, USA; corresponding author email: Terry.Henkel@humboldt. edu 3Department of Integrative Studies, Warren Wilson College, Asheville, NC 28815, USA 4Department of Botany & Plant Pathology, Purdue University, West Lafayette, IN 47907, USA Abstract: Jimtrappea guyanensis gen. sp. nov., Castellanea pakaraimophila gen. sp. nov., and Costatisporus Key words: cyanescens gen. sp. nov. are described as new to science. These sequestrate, hypogeous fungi were collected Boletineae in Guyana under closed canopy tropical forests in association with ectomycorrhizal (ECM) host tree genera Caesalpinioideae Dicymbe (Fabaceae subfam. Caesalpinioideae), Aldina (Fabaceae subfam. Papilionoideae), and Pakaraimaea Dipterocarpaceae (Dipterocarpaceae). Molecular data place these fungi in Boletaceae (Boletales, Agaricomycetes, Basidiomycota) ectomycorrhizal fungi and inform their relationships to other known epigeous and sequestrate taxa within that family. Macro- and gasteroid fungi micromorphological characters, habitat, and multi-locus DNA sequence data are provided for each new taxon. Guiana Shield Unique morphological features and a molecular phylogenetic analysis of 185 taxa across the order Boletales justify the recognition of the three new genera. Article info: Submitted: 31 May 2015; Accepted: 19 September 2015; Published: 2 October 2015. INTRODUCTION 2010, Gube & Dorfelt 2012, Lebel & Syme 2012, Ge & Smith 2013).
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												  TLC–Densitometry Analysis of Indole Compounds in Mycelial Culture of Imleria Badia and Agaricus Bisporus Enriched with Precursors — Serine Or Anthranilic AcidView metadata, citation and similar papers at core.ac.uk Originalbrought Research to you by PaperCORE provided by Jagiellonian Univeristy Repository TLC–Densitometry Analysis of Indole Compounds in Mycelial Culture of Imleria badia and Agaricus bisporus Enriched with Precursors — Serine or Anthranilic Acid Włodzimierz Opoka1*, Katarzyna Kała2, Remigiusz Krężałek2, Katarzyna Sułkowska-Ziaja2, Anna Maślanka1 and Bożena Muszyńska2 1Department of Inorganic Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, Kraków, Poland 2Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Kraków, Poland Received: 14 May 2017; accepted: 05 June 2017 Agaricus bisporus and Imleria in vitro cultures were cultivated on modified Oddoux medium, and Oddoux medium was enriched with serine or anthranilic acid. Serine or anthranilic acid was used at the concentrations of 0.1, 0.25, 0.5, and 0.75 g/L of medium. Determination of indole compounds in the obtained biomass was carried out using thin-layer chromatography (TLC) with densitometric detection. In every analyzed sample, presence of serine or anthranilic acid was studied. Comparison of the results obtained for the treatment and control samples allowed us to determine the optimum concentration of serine or anthranilic acid in the medium in order to obtain biomass with increased content of indole compounds. A. bisporus with addition of anthranilic acid or serine to the medium at the concentration of 0.5 g/L was the most beneficial. In the case of Imleria badia, anthranilic acid at the concentration of 0.5 g/L was the most optimal. This is the first report demonstrating the content of indole derivatives in biomass affected by their precursors (serine or anthranilic acid).
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												  The Mycological Society of San Francisco • Dec. 2015, Vol. 67:04The Mycological Society of San Francisco • Dec. 2015, vol. 67:04 Table of Contents Mushroom of the Month by K. Litchfield 1 Mushroom of the Month: Quick Start Forays Amanita muscaria by P. Koski 1 The Santa Mushroom, Fly Agaric President Post by B. Wenck-Reilly 2 Hospitality / Holiday Dinner 2015 4 Ken Litchfield Culinary Corner by H. Lunan 5 Brain Chemistry by B. Sommer 6 This month’s mushroom profile is one of my favorites, De- Mendo 2015 Camp by C. Haney 7 cember’s Santa mushroom. While prevalent at other times MycoMendoMondo by W. So 9 of the year in other places with more extensive rainy sea- Announcements / Events 10 sons, in the SF bay area the height of its season is the holi- 2015 Fungus Fair poster & program 11 days. One of the most elegant, beautiful, and recognizable Fungal Jumble & Gadget Obs by W. So 14 mushrooms in the world, the Santa mushroom is not only Cultivation Quarters by K. Litchfield 15 cosmopolitan and common, it is rich in lore and stately in Mushroom Sightings by P. Pelous 16 demeanor, yet cuddly and not lugubrious, just like Santa Calendar 17 himself. Decked in cheery cherry red and decoupaged with puffs of fluffy white, the Santa’s cap jingles atop its ivory bearded veil leading down the long white chimney stipe to URBAN PARK QUICK START FORAYS the skirty cummerbund constricting the top of the bulbous November 14 Quick Start Foray Report jolly belly. by Paul Koski One of the many There was hope for finding lots of fungi after fruits of the roots a couple of rainy days in the week before the foray but of the pine, the after some preliminary scouting in Golden Gate Park, Santa’s red and not many mushrooms were showing up.
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												  Mycorrhizal Fungi of Aspen Forests: Natural Occurrence and Potential ApplicationsUtah State University DigitalCommons@USU Aspen Bibliography Aspen Research 2001 Mycorrhizal fungi of aspen forests: natural occurrence and potential applications C.L. Cripps Follow this and additional works at: https://digitalcommons.usu.edu/aspen_bib Part of the Forest Sciences Commons Recommended Citation Cripps, CL. 2001. Mycorrhizal fungi of aspen forests: natural occurrence and potential applications. WD Shepperd et al (compilers). Sustaining Aspen in Western Landscapes: Symposium Proceedings. Proceedings RMRS-P-18. U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station. Fort Collins, CO. This Contribution to Book is brought to you for free and open access by the Aspen Research at DigitalCommons@USU. It has been accepted for inclusion in Aspen Bibliography by an authorized administrator of DigitalCommons@USU. For more information, please contact [email protected]. Mycorrhizal Fungi of Aspen Forests: Natural Occurrence and Potential Applications Cathy L. Cripps1 Abstract—Native mycorrhizal fungi associated with aspen were surveyed on three soil types in the north-central Rocky Mountains. Selected isolates were tested for the ability to enhance aspen seedling growth in vitro. Over 50 species of ectomycorrhizal fungi occur with Populus tremuloides in this region, primarily basidiomycete fungi in the Agaricales. Almost one-third (30%) were ubiquitous with aspen and were found on all three soil types. Over one-third (37%) were restricted to the acidic, sandy soil of the smelter-impacted Butte-Anaconda area, revealing a subset of fungi that tolerate these conditions. Mycorrhizal fungi were screened for their ability to enhance aspen growth and establishment. Of nine selected isolates, all but one increased the biomass of aspen seedlings 2–4 times.
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												  Svensk Mykologisk Tidskrift Volym 27 · Nummer 3 · 2006 Svensk Mykologisk Tidskrift Inkluderar TidigareSvensk Mykologisk Tidskrift Volym 27 · nummer 3 · 2006 Svensk Mykologisk Tidskrift inkluderar tidigare: www.svampar.se Svensk Mykologisk Tidskrift Sveriges Mykologiska Förening Tidskriften publicerar originalartiklar med svamp- Föreningen verkar för anknytning och med svenskt och nordeuropeiskt - en bättre kännedom om Sveriges svampar och intresse. Tidskriften utkommer med fyra nummer svampars roll i naturen per år och ägs av Sveriges Mykologiska Förening. - skydd av naturen och att svampplockning och Instruktioner till författare finns på SMF:s hemsi- annat uppträdande i skog och mark sker under da www.svampar.se. Svensk Mykologisk Tidskrift iakttagande av gällande lagar erhålls genom medlemskap i SMF. - att kontakter mellan lokala svampföreningar och svampintresserade i landet underlättas Redaktion - att kontakt upprätthålls med mykologiska före- Redaktör och ansvarig utgivare ningar i grannländer Mikael Jeppson - en samverkan med mykologisk forskning och Lilla Håjumsgatan 4, vetenskap. 461 35 TROLLHÄTTAN 0520-82910 Medlemskap erhålls genom insättning av med- [email protected] lemsavgiften 200:- (familjemedlem 30:-, vilket ej inkluderar Svensk Mykologisk Tidskrift) på postgi- Hjalmar Croneborg rokonto 443 92 02 - 5. Medlemsavgift inbetald Mattsarve Gammelgarn från utlandet är 250:-. 620 16 LJUGARN 018-672557 Subscriptions from abroad are welcome. [email protected] Payments (250 SEK) can be made to our bank account: Jan Nilsson Swedbank (Föreningssparbanken) Smultronvägen 4 Storgatan, S 293 00 Olofström, Sweden 457 31 TANUMSHEDE SWIFT: SWEDSESS 0525-20972 IBAN no. SE9280000848060140108838 [email protected] Äldre nummer av Svensk Mykologisk Tidskrift Sveriges Mykologiska Förening (inkl. JORDSTJÄRNAN) kan beställas från SMF:s Botaniska Institutionen hemsida www.svampar.se eller från föreningens Göteborgs Universitet kassör. Box 461 Previous issues of Svensk Mykologisk Tidskrift 405 30 Göteborg (incl.
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												  Field Key to the Boletes of CaliforniaField Key to the Boletes of California Key to the Genera of Boletes 1. Tubes typically disoriented and irregularly arranged; spore deposit not obtainable ........ Gastroboletus 1. Tubes more or less vertically oriented and orderly arranged; spore deposit usually readily obtainable ...................................................................................................................................................................... 2 2. Basidiocarps small (4‐7 cm); tubes white when young, becoming bright yellow at maturity; spore deposit yellow; stipe typically hollow in the basal portion with age ...................................... ........................................................................................................................ Gyroporus castaneus 2. Basidiocarps typically larger; tubes yellow when young, or if white at first, then not bright yellow with age; spore deposit olivaceous to brown to reddish brown or flesh or vinaceous color; stipe usually not hollow ........................................................................................................ 3 3. Basidiocarp with a conspicuous, cottony, bright yellow veil (be sure to check young specimens) .......... ................................................................................................................................ Pulveroboletus ravenelii 3. Basidiocarps lacking such a veil ............................................................................................................... 4 4. Spore deposit flesh