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GUIDANCE MANUAL Model 900 BGI 900 HIGH VOLUME CASCADE IMPACTOR

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GUIDANCE MANUAL Model 900 BGI 900 HIGH VOLUME CASCADE IMPACTOR GUIDANCE MANUAL Model 900 BGI 900 HIGH VOLUME CASCADE IMPACTOR BGI Incorporated 58 Guinan Street Waltham, MA 02451 USA Tel : 781 891-9380 Fax: 781 891-8151 www.bgiusa.com [email protected] Version 1.0.0 December, 2008 Table of Contents Patents, Copyrights and Trademarks .................................................................... 1 Safety Notice ......................................................................................................... 2 Warranty (U.S) ...................................................................................................... 3 Operation manual Revision List ............................................................................ 4 Section 1. Introduction .......................................................................................... 5 Section 2. Hardware Installation ........................................................................... 8 2.1 Description of the BGI900 .................................................................. 8 2.2 Description of the Sampling Pump ..................................................... 11 2.3 Description of the PUF Substrate & Filter Material ........................... 13 2.4 System Setup ....................................................................................... 15 2.4.1 Cleaning the Glassware & Tools ...................................................... 16 2.4.1.1 Thermal Cleaning .......................................................................... 16 2.4.1.2 Solvent Cleaning .......................................................................... 17 2.4.2 Preparing the PUF Substrate ........................................................... 18 2.4.2.1 Cleaning the PUF Substrates ....................................................... 19 2.4.2.2 Storing the Clean, Dry PUF Substrate .......................................... 21 2.4.2.3 Cleaning the Substrate Holders .................................................... 22 2.4.2.4 Cleaning the Ultrafine Filter Hplders ........................................... 24 2.4.2.5 Preparing the PM-10 Grease Collection Platform ........................ 25 2.4.3 Assembling the Collection Stages ................................................... 25 2.4.4 Installing the Sampler Onto the Tripod ........................................... 34 2.4.5 Setting Up the Pump ........................................................................ 36 Section 3. Post Sampling Analysis ....................................................................... 38 3.1 Removal of the Collection Platform ................................................... 38 3.2 Substrate Extraction for Analysis of Water Soluble Compmnents .... 42 3.2.1 Labware Washing ............................................................................ 43 3.2.2 Extracting PUF Substrates with Milli-Q Water ............................... 44 3.2.2.1 PM-10 PUF Substrate (P1 Size) ................................................... 44 3.2.2.2 PM-2.5 PUF Substrates (P2 Size) ................................................ 46 3.2.2.3 PM-1.0, PM-0.5 & PM-0.1 PUF Substrates (P3 Size) ................. 48 3.2.2.4 Ultrafine Filter (Ultrafilter) .......................................................... 50 3.3 Substrate Extraction for Toxicological Testing ................................. 51 3.3.1 Labware Washing ............................................................................ 52 3.3.2 Extracting PUF Substrates with Methanol ....................................... 53 3.3.2.1 PM-10 PUF Substrate (P1 Size) .................................................... 53 3.3.2.2 PM-2.5 PUF Substrates (P2 Size) ................................................. 55 3.3.2.3 PM-1.0, PM-0.5 & PM-0.1 PUF Substrates (P3 Size) .................. 57 3.3.3 Evaporation of Solvent from PUF Extracts ...................................... 59 3.3.4 Extracting Ultrafilters with Methanol ............................................... 61 3.3.5 Evaporation of Solvent from Ultrafine Extracts ............................... 63 3.4 Analysis of Samples ............................................................................. 67 References ................................................................................................... 68 Patents, Copyrights and Trademarks This instrument from BGI Incorporated (BGI) is covered by one or more of the following US patents: U.S. Patent Office Number 6,435,043 Harvard School of Public Health This instrument is fabricated, distributed and technically supported by BGI Incorporated. The technical design is from the Harvard School of Public Health (HSPH) and BGI is the sole licensed manufacturer of the High Volume Cascade Impactor, BGI Model 900. This Guidance Manual may contain trade secrets and confidential information proprietary to BGI and HSPH. The documentation and information contained therein may not be used, duplicated or disclosed to anyone, in whole or in part, other than as authorized by Agreement and with the express written permission of BGI. © 2008 BGI Incorporated. All rights reserved throughout the world. Note: The former product name of this instrumentation was “CHEMVOL©” since 2003, and was formerly a registered trademark of Rupprecht & Patashnick Company Incorporated©. 1 Safety Notice Repair of instrumentation manufactured by BGI Incorporated (BGI) should only be attempted by properly trained service personnel. Do not tamper with this hardware. Use established safety precautions when working with this instrument. BGI and its licensed distributors can not foresee all possible modes of operation in which the user may attempt to utilize this instrument. The user assumes all liability associated with the use of the instrument. BGI and its distributors further disclaim any responsibility for consequential damages. Use of this product in any manner not intended by BGI, the manufacturer, will void the safety protection provided by the equipment, and may damage the equipment and subject the user to injury. 2 Warranty (U.S.) BGI warrants equipment of its manufacture and bearing its nameplate to be free from defects in workmanship and material. We make no warranty, express or implied, except as set forth herein. BGI's liability under this warranty extends for a period of one (1) year from the date of BGI's shipment. It is expressly limited to repairing or replacing at the factory during this period and at BGI's option, any device or part which shall within one year of delivery to the original purchaser, be returned to the factory, transportation prepaid and which on examination shall in fact be proved defective. BGI assumes no liability for consequential damages of any kind. The purchaser, by acceptance of this equipment, shall assume all liability for consequences of its misuse by the purchaser, his employees or others. No information derived from the use of this equipment is warranted for any purpose whatsoever. This warranty will be void if the equipment is not handled, installed, or operated in accordance with our instructions. If damage occurs during transportation to the purchaser, BGI must be notified immediately upon arrival of the equipment. The Equipment will be returned via collect shipment. A defective part in the meaning of this warranty shall not, when such part is capable of being repaired or replaced, constitute a reason for considering the complete equipment defective. Acknowledgment and approval must be received from BGI prior to returning parts or equipment for credit. BGI makes engineering changes and improvements from time to time on instruments of its manufacture. We are under no obligation to retrofit these improvements and/or changes into instruments which have already been purchased. No representative of ours has the authority to change or modify this warranty in any respect. 3 Operation Manual Revision List As BGI instrumentation fabrication and design changes, so do our Guidance Documents and Operator Manuals. However, these changes may affect only one aspect of an instrument, while leaving the instrument as a whole unchanged. To explain these individual changes to BGI users, BGI will update only these sections of its Guidance Document or Operator Manual that are affected by the instrument updates or improvements. When this manual changes, so does it revision number. The Guidance Document Revision Number is located on the front cover. The latest versions of any BGI manual may be found on our website: http://www.bgiusa.com/cau/manuals_2005.htm To assist users of the High Volume Cascade Impactor track the future changes, the following list of manual sections with its respective revision number are listed: Section Number Description of Change Date Guidance Document “Chemvol© Model 2400 May 2003 (Discontinued) (Replaced With) Model 900 High Volume Cascade Impactor December 2008 4 Section 1. INTRODUCTION The High Volume Cascade Impactor (Figure1-1), manufactured by BGI Incorporated (BGI) is a licensed and patented instrument design of the Harvard University School of Public Health (HSPH). The initial design, testing and verification of the High Volume Cascade Impactor was conducted by HSPH. BGI is the sole licensed manufacturer and distributor of this product. The BGI High Volume Cascade Impactor (HVCI) is designed to aerodynamically classify the particles in the ambient air by the principle of Cascade Impaction. A set of Orifice Stages with decreasing orifice diameters are placed sequentially on top of one another and ambient air is drawn through
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