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Microinjection & Transfection 2016-2017
WORLD PRECISION INSTRUMENTS Instrumenting scientific ideas Microinjection & Transfection 2016-2017 Come see us at The Allied Genetics 2016 Conference • July 13-17 • Booths # 623 & 816/818 Everything you need for Microinjection Microinjection processes use either metal microinjection Recently, WPI introduced its customizable Microinjection needles or glass micropipettes to inject small liquid System with everything you need to get started. We can volumes. For example, genetic material may be inserted help you customize your system with many options and into a living cell, a drug introduced into an eye or brain, microinjection system accessories. The basic system or fluid injected into a muscle. Typically, microinjection includes: is performed under a microscope. A stereotaxic frame l Microinjection pump like the PV820 Pneumatic setup may be required. PicoPump l LED lighted microscope base with a PZMIII Stereo Microscope and an articulating mirror l Micromanipulator l Many accessories WPI offers a variety of pumps along with special syringes, stereotaxic frames, glass capillaries and needles. The setup you choose depends on the size of your microinjection aliquots, the volume to be injected and the size of needle or glass tip you choose. In addition, we offer an electroporator for transfection procedures. Whether you are working with Danio rerio (zebrafish), Xenopus, Drosophila or Caenorhabditis elegans, we can help you customize a microinjection system for your application. Options for Customizing Your System INJECTOR ✱ PV820 Pneumatic PicoPump with Hold Pressure • PV830 Pneumatic PicoPump with Hold Designed to simplify intracellular injection and a variety Microprocessor- The versatile Pressure and Vacuum of other micro in jec tion tasks, WPI's PicoPumps use UMP3 controlled injector uses • Nanoliter2010 carefully regulated air pres sures for se cur ing cells and injecting them with fluid. -
Laboratory Equipment Reference Sheet
Laboratory Equipment Stirring Rod: Reference Sheet: Iron Ring: Description: Glass rod. Uses: To stir combinations; To use in pouring liquids. Evaporating Dish: Description: Iron ring with a screw fastener; Several Sizes Uses: To fasten to the ring stand as a support for an apparatus Description: Porcelain dish. Buret Clamp/Test Tube Clamp: Uses: As a container for small amounts of liquids being evaporated. Glass Plate: Description: Metal clamp with a screw fastener, swivel and lock nut, adjusting screw, and a curved clamp. Uses: To hold an apparatus; May be fastened to a ring stand. Mortar and Pestle: Description: Thick glass. Uses: Many uses; Should not be heated Description: Heavy porcelain dish with a grinder. Watch Glass: Uses: To grind chemicals to a powder. Spatula: Description: Curved glass. Uses: May be used as a beaker cover; May be used in evaporating very small amounts of Description: Made of metal or porcelain. liquid. Uses: To transfer solid chemicals in weighing. Funnel: Triangular File: Description: Metal file with three cutting edges. Uses: To scratch glass or file. Rubber Connector: Description: Glass or plastic. Uses: To hold filter paper; May be used in pouring Description: Short length of tubing. Medicine Dropper: Uses: To connect parts of an apparatus. Pinch Clamp: Description: Glass tip with a rubber bulb. Uses: To transfer small amounts of liquid. Forceps: Description: Metal clamp with finger grips. Uses: To clamp a rubber connector. Test Tube Rack: Description: Metal Uses: To pick up or hold small objects. Beaker: Description: Rack; May be wood, metal, or plastic. Uses: To hold test tubes in an upright position. -
Corning Life Sciences Selection Guide Issue 6
www.corning.com/lifesciences Corning Life Sciences Selection Guide Issue 6 Trust Corning for Your Research For superior quality and performance in life science research tools, you can count on Corning. Our comprehensive line of advanced products and technologies is designed to meet your evolving needs in cell-based and genomics research. To learn more, please visit www.corning.com/lifesciences, or call 1.800.492.1110. Customers outside the U.S., please call 1.978.442.2200 or contact your local support office (listed below). Corning Incorporated Worldwide India Taiwan United Kingdom Life Sciences Support Offices t 91-124-235 7850 t 886 2-2716-0338 t 0800 376 8660 f 91-124-401 0207 f 886 2-2716-0339 f 0800 279 1117 Corning BV Life Sciences ASIA/PACIFIC Japan All Other European EUROPE Koolhovenlaan 12 Australia t 81 (0) 3-3586 1996/1997 Countries 1119 NE Schiphol Rijk t 61 2-9416-0492 f 81 (0) 3-3586 1291/1292 France t 31 (0) 20 659 60 51 The Netherlands f 61 2-9416-0493 Korea t 0800 916 882 f 31 (0) 20 659 76 73 f 0800 918 636 Corning Incorporated China t 82 2-796-9500 f 82 2-796-9300 Germany LATINAMERICA Life Sciences t 86 21-3222-4666 t 0800 101 1153 Brasil Tower 2, 4th Floor f 86 21-6288-1575 Singapore f 0800 101 2427 t (55-11) 3089-7419 900 Chelmsford St. Hong Kong t 65 6733-6511 The Netherlands f (55-11) 3167-0700 Lowell, MA 01851 t 852-2807-2723 f 65 6861-2913 Mexico t 800.492.1110 f 852-2807-2152 t 31 20 655 79 28 New! t (52-81) 8158-8400 t 978.442.2200 f 31 20 659 76 73 f (52-81) 8313-8589 f 978.442.2476 www.corning.com/lifesciences Online ordering now available 6/08 6M APC CLS–PSG–001-A4-REV6 Corning, Costar, CellBIND, CellCube, CellSTACK, DNA-BIND, Lambda, Octapette, Spin-X, Stripette, Stripwell, Thermowell, Transtar-96, Transwell,, 8-Pette, and 12-Pette are registered trademarks of Corning Incorporated, Corning, NY. -
Product Overview
Product Overview Electrophysiology 2020 • Patch Clamp Amplifier • Electrophysiological Accessories • Two Electrode Voltage Clamp • Electrical Shielding • Automated Patch Clamp Systems • Pipette & Electrode Manufacturing • Data Acquisition Boards • Software www.heka.com For over 45 years HEKA has designed and manufactured sophisticated instrumentation and software for biomedical and industrial research applications. Through the years, HEKA has achieved an unparalleled reputation for precision and quality. Medical, pharmaceutical and industrial research facilities world-wide rely on HEKA ingenuity for their discoveries. While there have been many changes in research, instrumentation, and software, our commitment to bring innovative technology to our customers remains constant. HEKA is a select group of engineers, biomedical researchers, and computer scientists who pride themselves on the quality of HEKA products. HEKA offers complete pre- and post-sales technical support, and takes care of each customer personally. In every way, HEKA provides solutions. HEKA Elektronik GmbH is proud to be part of the Smart Ephys umbrella. Together with our other Harvard Bioscience, Inc. brands Multi Channel Systems GmbH and Warner Instruments we offer complete solutions for electrophysiology. You will find the high-quality products and service that you know and trust from each of the individual companies, but you can get information on all products, complete set-ups (e.g. patch clamp rigs), and product consultation from one source. Please check out the Smart Ephys website and contact your local sales representative with any questions. Buy Online The Smart Ephys one-stop-shop concept applies to online orders as well. Quickly order consumables and accessories via our webshop. Expedite equipment acquisition by generating your own quote for your purchasing department. -
Laboratory Safety Regulations Humboldt State University • Department of Chemistry Lower Level
Version 3.0 September 28,, 2015 Laboratory Safety Regulations Humboldt State University • Department of Chemistry Lower Level FAILURE TO OBSERVE SAFETY PRECAUTIONS MAY RESULT IN IMMEDIATE DISMISSAL FROM THE LABORATORY AND ASSOCIATED LECTURE! A. General 1. FirstAid kits are available in the following locations: Main stockroom—SA 569; Organic Stockroom—SA 566; Analytical/Inorganic/Physical Lab—SA 369. Bandaids for minor cuts are available in the main stockroom. 2. Notify your instructor as soon as possible after all accidents and/or injuries regardless of their severity. If you need medical treatment, you will be promptly taken to the Student Health Center. In case of accident after 5:00 pm or on weekends, call the campus police at 911. 3. Perform no unauthorized experiments. 4. Horseplay, pranks, and other acts of mischief are strictly prohibited and will result in immediate dismissal from the laboratory. 5. Work with chemicals only after you have learned about their potential hazards. Then, proceed with caution. 6. There are always risks when working with chemicals so work cautiously and defensively. Safety Data Sheets (SDS) are available in the Main Stockroom and are also available on the Web. 7. You are required to determine the hazards of any chemical before you use the chemical. For example, ask yourself the following: ● What are the greatest risks from using this chemical? How can I minimize these risks? ● In what form is this chemical most hazardous? Least hazardous? ● How can I arrange my work so that the chemical is used in the least hazardous manner? ● If I have to transport this chemical, what is the safest way to do so? ● How would I respond if the chemical were spilled? 8. -
Building a Morbidostat: an Automated Continuous-Culture Device For
PROTOCOL Building a morbidostat: an automated continuous- culture device for studying bacterial drug resistance under dynamically sustained drug inhibition Erdal Toprak1,2, Adrian Veres3, Sadik Yildiz2, Juan M Pedraza4, Remy Chait1, Johan Paulsson1,5 & Roy Kishony1,5 1Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA. 2Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey. 3Health Sciences and Technology Program, Harvard Medical School, Boston, Massachusetts, USA. 4Department of Physics, Universidad de los Andes, Bogotá, Colombia. 5School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA. Correspondence should be addressed to E.T. ([email protected]) or R.K. ([email protected]). Published online 21 February 2013; doi:10.1038/nprot.2013.021 We present a protocol for building and operating an automated fluidic system for continuous culture that we call the ‘morbidostat’. The morbidostat is used to follow the evolution of microbial drug resistance in real time. Instead of exposing bacteria to predetermined drug environments, the morbidostat constantly measures the growth rates of evolving microbial populations and dynamically adjusts drug concentrations inside culture vials in order to maintain a constant drug-induced inhibition. The growth rate measurements are done using an optical detection system that is based on measuring the intensity of back-scattered light from bacterial cells suspended in the liquid culture. The morbidostat can additionally be used as a chemostat or a turbidostat. The whole system can be built from readily available components within 2–3 weeks by biologists with some electronics experience or engineers familiar with basic microbiology. INTRODUCTION Antibiotic resistance is an important public health problem, ren- at a constant rate lower than the maximal growth rate of the popu- dering currently available drugs useless and threatening millions lation, the dilution rate of the morbidostat rdilution ≅ ∆V/(V·∆t) is of lives1–4. -
Laboratory Equipment Used in Filtration
KNOW YOUR LAB EQUIPMENTS Test tube A test tube, also known as a sample tube, is a common piece of laboratory glassware consisting of a finger-like length of glass or clear plastic tubing, open at the top and closed at the bottom. Beakers Beakers are used as containers. They are available in a variety of sizes. Although they often possess volume markings, these are only rough estimates of the liquid volume. The markings are not necessarily accurate. Erlenmeyer flask Erlenmeyer flasks are often used as reaction vessels, particularly in titrations. As with beakers, the volume markings should not be considered accurate. Volumetric flask Volumetric flasks are used to measure and store solutions with a high degree of accuracy. These flasks generally possess a marking near the top that indicates the level at which the volume of the liquid is equal to the volume written on the outside of the flask. These devices are often used when solutions containing dissolved solids of known concentration are needed. Graduated cylinder Graduated cylinders are used to transfer liquids with a moderate degree of accuracy. Pipette Pipettes are used for transferring liquids with a fixed volume and quantity of liquid must be known to a high degree of accuracy. Graduated pipette These Pipettes are calibrated in the factory to release the desired quantity of liquid. Disposable pipette Disposable transfer. These Pipettes are made of plastic and are useful for transferring liquids dropwise. Burette Burettes are devices used typically in analytical, quantitative chemistry applications for measuring liquid solution. Differing from a pipette since the sample quantity delivered is changeable, graduated Burettes are used heavily in titration experiments. -
Proceedings of the Indiana Academy of Science
m A Simple Apparatus for the Study of Phototropic Responses in Seedlings. Geo. N. Hoffer. The purpose of this apparatus is to determine the minimum (piantity of light, acting as a lateral stimulus, that will produce a curvature response in seedlings of various kinds as well as some of the fungi, such as Fhj/comyccs and PUoJxAuh. Any kind of light may lie used, Iiut in the comparative studies I use direct sunlight. The <iuantity of liglit is regulated by opening and closing an iris diaphragm with various-sized apertures for definite lengths of time. The apparatus is made from a microscope carrying case. As shown in the photograph, Fig. 1, the outside attachments are the drawtube and rack and pinion of a microscope removed from the base and attached to one side of the box. Tlie tube works through a hole in the side of the box. The opening is made light proof by a velvet collar. Fig. III. \(\ glued to the rim and held to the tube of the scope by rubber I)ands. Into anotlu'r hole is Gttid a hemispheric, revolving iris diaphragm. Fig. Ill, I. This is on the adjacent side of the box close to the microscope and en the same level with the objective of the microscope. A mirror is at- tached to the box to reflect light directly into it through the iris and onto the plant. A micrometer eyepiece in tlie microscope is the index by which all of the readings are made. The illumination for the readings is supplied by the light which passes through the bottle, Fig. -
Chemistry 2A Lab Manual Standard Operating Procedures Winter Quarter 2018
Chemistry 2A Lab Manual Standard Operating Procedures Winter Quarter 2018 Department of Chemistry University of California - Davis Davis, CA 95616 Student Name Locker # Laboratory Information Teaching Assistant’s Name Laboratory Section Number Laboratory Room Number Dispensary Room Number 1060 Sciences Lab Building Location of Safety Equipment Nearest to Your Laboratory Safety Shower Eye Wash Fountain Fire Extinguisher Fire Alarm Safety Chemicals Revision Date 12/1/2017 Preface Chemistry is an experimental science. Thus, it is important that students of chemistry do experiments in the laboratory to more fully understand that the theories they study in lecture and in their textbook are developed from the critical evaluation of experimental data. The laboratory can also aid the student in the study of the science by clearly illustrating the principles and concepts involved. Finally, laboratory experimentation allows students the opportunity to develop techniques and other manipulative skills that students of science must master. The faculty of the Chemistry Department at UC Davis clearly understands the importance of laboratory work in the study of chemistry. The Department is committed to this component of your education and hopes that you will take full advantage of this opportunity to explore the science of chemistry. A unique aspect of this laboratory program is that a concerted effort has been made to use environmentally less toxic or non-toxic materials in these experiments. This was not only done to protect students but also to lessen the impact of this program upon the environment. This commitment to the environment has presented an enormous challenge, as many traditional experiments could not be used due to the negative impact of the chemicals involved. -
Solution Bottles NEW 500Ml Square Media Bottle Erlenmeyer & Fernbach Flasks
Erlenmeyer & Fernbach Flasks Solution Bottles • Compatible with 1 bottle top filters or for use as solution storage 1 containers (Bottle Top Filters sold separately, see page 35) 1 Solution bottle neck size is 45mm 2 Curved bottle design 3 2 for easy handling 2 SOLUTION BOTTLES S, R/D F, NP, USP, PS Working Pack Qty/ Part No. Product Description volume Description Case 229781 150mL Solution Bottle 150mL 1/Bag 24 229782 250mL Solution Bottle 250mL 1/Bag 24 229784 500mL Solution Bottle 500mL 1/Bag 24 4 229785 1000mL Solution Bottle 1000mL 1/Bag 24 • Designed for mammalian suspension culture applications as well as bacterial & yeast culture NEW 500mL Square Media Bottle • Conveniently pre-sterilized 1 70mm opening for easy filling and dispensing (Erlenmeyer & Fernbach) 2 2 Over-sized vent area in cap for improved gas exchange (Erlenmeyer & Fernbach) • Ideal for media prep and storage of laboratory reagents and biological buffers 3 Fernbach flask shape increases surface area to volume ratio • Square design saves valuable shelf space and is ideal for 4 Baffled bottom improves mixing and aeration stacking, such as in a refrigerator • Plain bottom also available Part No. 229896 - Transfer Assembly • Unique individually bagged packaging allows opening one at a time ERLENMEYER & FERNBACH FLASKS S, NP, USP, A, PC 1 • PETG material reduces gas permeability Cap Bottom Max. Pack Qty/ Part No. Product Description Type Type Volume Description Case 1 Molded graduations on two sides 229850 2L Erlenmeyer Flask Vent Plain 2200mL 1/Bag 6 229855 2L Erlenmeyer Flask -
Corning Cell Culture Selection Guide Corning Is Helping to Make Your Research Possibilities Real with New and Innovative Products
Corning Cell Culture Selection Guide Corning is helping to make your research possibilities real with new and innovative products. Introduction We have recently advanced our microplate line to include many enhancmements and new products. Check out the Corning is pleased to present our Life Sciences Selection Guide. In this guide, you will find a selection of our newest, new 384 well solid, low-volume and Poly-D-Lysine micro- most innovative and most requested products. plates, as well as 384 and 1536 well microplates with generic bar codes in the Microplates Section. For more than 150 years, Corning Incorporated has leveraged its materials science and process engineering expertise to For up-to-date information on Corning Life Sciences’ collaborate closely with customers worldwide — turning comprehensive range of products and services, go to what were once only possibilities into breakthrough realities. www.corning.com/lifesciences where you can access: Q New Products Information One such reality is the Corning® Epic® System, a high- throughput label-free screening platform based on optical Q Technical Information including: biosensor technology.The system performs both biochemical - Application Notes and cell-based drug discovery applications and offers drug - Instruction Manuals developers the ability to evaluate promising new drug - Product Bulletins targets. It also allows for the observation of direct biological Q Educational Opportunities interactions not previously detectable in high-throughput Q Product Catalog Information applications. Q Product Literature For hard-to-attach cell lines, Corning offers a number of Q Complete Distributor Information modified or synthetic surfaces including Corning CellBIND® Surface, Ultra-Low Attachment, and Ultra-Web™ Surfaces. -
EXTRACTING DNA (DNA = Deoxyribonucleic Acid) from COMMON FOOD INGREDIENTS
ACTIVITY: EXTRACTING DNA (DNA = deoxyribonucleic acid) FROM COMMON FOOD INGREDIENTS DNA Extraction – Onion method #1: Ingredients: Isopropyl alcohol* Ice/water bath ½ onion distilled water ¼ tsp sodium chloride (table salt) (1.5 g) 1 mL ¾ tsp sodium hydrogen carbonate (baking soda) (5 g) 4 mL 1 tsp name brand dish soap or liquid detergent 5 mL *traditional experiments recommend 99%, but this is not readily available; 70% is readily available from most pharmacies Materials: 1 paring knife 1 cutting board Ice bucket or container for ice solution Measuring cups or 125 mL and 250 mL Erlenmeyer flasks Strainer or funnel Paper towels or small coffee filters Glass stirring rods or wooden stir sticks Clear bowls or test tubes 1. Place isopropyl alcohol in ice/water bath or in the freezer to cool thoroughly. 2. Buffer solution: 125 mL of water (distilled water, if available) ½ cup 1 mL (1.5 grams) of sodium chloride (table salt) ¼ tsp 4 mL (5.0 grams) baking soda (sodium hydrogen carbonate) ¾ tsp 5 mL of good quality liquid laundry detergent, 1 tsp Make the buffer solution by pouring placing all the ingredients into a clean 250 mL Erlenmeyer flask or clean container. Chill the buffer solution by placing the flask in a larger beaker filled with crushed ice and water. Note: buffer solutions are used in this lab for several reasons. • the saltiness and acidity (pH) of the solution is very close to that in living things; as a result, the DNA will like to dissolve into this solution. • the detergent is added to help break down cell walls in the onion cells.