Chemistry 2A Lab Manual Standard Operating Procedures Winter Quarter 2018
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FS/2018 ORGANIC CHEMISTRY I LAB CHEM 226 (2219) Instructor
FS/2018 ORGANIC CHEMISTRY I LAB CHEM 226 (2219) Instructor: Terry Bone 120-B Schrenk Hall [email protected] 341-4820 http://www.mst.edu/~tbone 9:00-10:00, Tu-Th, or by appt. Cynthia Bolon 213 Schrenk Hall [email protected] 341-4439 OBJECTIVES: Course objectives are to develop facility with performing laboratory techniques involving the handling of organic chemicals safely and the keeping of proper records of experiments conducted in the laboratory. Crystallization, distillation, extraction and chromatography are emphasized as separation and purification techniques. Melting points, boiling points, and refractive indices are used routinely as measures of purity. Safe handling of chemicals and proper disposal of waste products are priority goals in this course. To help in attaining such goals, the first week of the semester is devoted to SAFETY in the chemistry laboratory. For the remainder of the semester, most of the experiments are conducted on a micro scale. BOOKS: 1. 100 pg. set Organic Chemistry Laboratory Notebook 2. “Organic Chemistry Laboratory Laboratory Techniques, 2nd ed”. Available as free pdf download at https://open.umn.edu/opentextbooks/BookDetail.aspx?bookId=369 3. “Microscale Techniques for the Organic Laboratory, 2nd ed”, (MTOL), Mayo, Pike, Butcher and Trumper, John Wiley & Sons, Inc.,2001 (on reserve at library circulation desk-currently out of print but available on Amazon) 4. “Right to Know pocket guide for School & University Employees”, Genium Publishing Corp., 1990. (optional) (#1 can be purchased from the book store) LAB SYLLABUS: Originally prepared by Prof. S. B. Hanna, modified by D. E. Hoiness, T.Bone. GENERAL GUIDELINES SAFETY Goggles must be worn at all times in the lab. -
White Paper Absorbance Or Fluorescence: Which Is the Best
WHITE PAPER No. 40 Absorbance or Fluorescence: Which Is the Best Way to Quantify Nucleic Acids? Natascha Weiß1, Martin Armbrecht1 ¹Eppendorf AG, Hamburg, Germany Executive Summary When performing molecular experiments on nucleic acids, it is a basic requirement to determine the concentration as well as the quality of the sample. The standard method which serves this purpose is UV-Vis spectrophotometry – but is it always the right choice? In the present White Paper, the advantages and disadvantages of this technique will be described and it will be compared with nucleic acid quantification via fluorescence. In this context, it will be discussed which situations warrant the use of which one of the two methods. The decision will generally depend on the condition of the sample as well as on the requirements of downstream applications. Since the advantages of both methods complement each other well, it is most practical to have the ability to perform both methods on a single instrument in a flexible manner. Introduction Experiments involving nucleic acids are a mainstay in any evaluated by measuring the sample at additional wave molecular laboratory. DNA and RNA are isolated from micro- lengths (230 nm, 280 nm) and calculating the purity ratios, organisms and from cells of higher order organisms in order i.e. the ratios of the values obtained at 260/230 nm and at to be employed in a broad variety of processing steps and 260/280 nm, respectively. In this way, it will be evident analyses. It is crucial for any type of downstream applica- whether cellular debris or remainders of reagent used during tion that a defined amount of nucleic acid is used and that purification such as proteins, sugar molecules, certain salts the sample is free from contaminations that may impact the or phenols, are present in the solution, as these will generate experiments. -
EWRE Lab Regulations
Department of Civil & Environmental Engineering University of Massachusetts College of Engineering University of Massachusetts September 2012 CONTENTS UNIVERSITY OF MASSACHUSETTS ......................................................................................................................... 1 CONTENTS ...................................................................................................................................................................... 2 A. LABORATORY SAFETY AND SECURITY ........................................................................................................... 1 1. GENERAL RULES .......................................................................................................................................................... 1 2. EMERGENCY ................................................................................................................................................................ 2 3. DISPOSAL OF CHEMICALS ............................................................................................................................................ 2 a. What chemical wastes are hazardous or non-hazardous? ...................................................................................... 2 b. Disposal of hazardous wastes ................................................................................................................................. 2 4. FUME HOODS .............................................................................................................................................................. -
On Food, Spectrophotometry, and Measurement Data Processing (Keynote Lecture)
12th IMEKO TC1 & TC7 Joint Symposium on Man Science & Measurement September, 3–5, 2008, Annecy, France ON FOOD, SPECTROPHOTOMETRY, AND MEASUREMENT DATA PROCESSING (KEYNOTE LECTURE) Roman Z. Morawski Warsaw University of Technology, Faculty of Electronics and Information Technology, Institute of Radioelectronics Warsaw, Poland [email protected] Abstract: Spectrophotometry is getting more and more Despite philosophical abnegation of food issues, the often the method of choice not only in laboratory analysis of practice of food preparation and refinement has flourished (bio)chemical substances, but also in the off-laboratory for centuries, and inspired research-and-invention-oriented identification and testing of physical properties of various minds. Today, we may speak about a fully developed products, in particular – of various organic mixtures discipline of science and technology. Enough to say that the including food products and ingredients. Specialized Institute of Food Technologists – the largest international, spectrophotometers, called spectrophotometric analyzers are non-profit professional organization involved in the designed for such applications. This keynote lecture is on advancement of food science and technology – is the state of the art and developmental trends in the domain encompassing 23 000 members worldwide. Its Committee of spectrophotometric analyzers of food with particular on Higher Education provided the following definition of emphasis on wine analyzers. The following issues are food science: "Food science is the discipline in which the covered: philosophical and methodological background of engineering, biological, and physical sciences are used to food analysis, physical and metrological principles of study the nature of foods, the causes of deterioration, the spectrophotometry, the role of measurement data processing principles underlying food processing, and the improvement in spectrophotometry, food analyzers on the market and of foods for the consuming public" [1]. -
ELISA Plate Reader
applications guide to microplate systems applications guide to microplate systems GETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS SALES OFFICES United States Molecular Devices Corp. Tel. 800-635-5577 Fax 408-747-3601 United Kingdom Molecular Devices Ltd. Tel. +44-118-944-8000 Fax +44-118-944-8001 Germany Molecular Devices GMBH Tel. +49-89-9620-2340 Fax +49-89-9620-2345 Japan Nihon Molecular Devices Tel. +06-6399-8211 Fax +06-6399-8212 www.moleculardevices.com ©2002 Molecular Devices Corporation. Printed in U.S.A. #0120-1293A SpectraMax, SoftMax Pro, Vmax and Emax are registered trademarks and VersaMax, Lmax, CatchPoint and Stoplight Red are trademarks of Molecular Devices Corporation. All other trademarks are proprty of their respective companies. complete solutions for signal transduction assays AN EXAMPLE USING THE CATCHPOINT CYCLIC-AMP FLUORESCENT ASSAY KIT AND THE GEMINI XS MICROPLATE READER The Molecular Devices family of products typical applications for Molecular Devices microplate readers offers complete solutions for your signal transduction assays. Our integrated systems γ α β s include readers, washers, software and reagents. GDP αs AC absorbance fluorescence luminescence GTP PRINCIPLE OF CATCHPOINT CYCLIC-AMP ASSAY readers readers readers > Cell lysate is incubated with anti-cAMP assay type SpectraMax® SpectraMax® SpectraMax® VersaMax™ VMax® EMax® Gemini XS LMax™ ATP Plus384 190 340PC384 antibody and cAMP-HRP conjugate ELISA/IMMUNOASSAYS > nucleus Single addition step PROTEIN QUANTITATION cAMP > λEX 530 nm/λEM 590 nm, λCO 570 nm UV (280) Bradford, BCA, Lowry For more information on CatchPoint™ assay NanoOrange™, CBQCA kits, including the complete procedure for this NUCLEIC ACID QUANTITATION assay (MaxLine Application Note #46), visit UV (260) our web site at www.moleculardevices.com. -
Laboratory Equipment Reference Sheet
Laboratory Equipment Stirring Rod: Reference Sheet: Iron Ring: Description: Glass rod. Uses: To stir combinations; To use in pouring liquids. Evaporating Dish: Description: Iron ring with a screw fastener; Several Sizes Uses: To fasten to the ring stand as a support for an apparatus Description: Porcelain dish. Buret Clamp/Test Tube Clamp: Uses: As a container for small amounts of liquids being evaporated. Glass Plate: Description: Metal clamp with a screw fastener, swivel and lock nut, adjusting screw, and a curved clamp. Uses: To hold an apparatus; May be fastened to a ring stand. Mortar and Pestle: Description: Thick glass. Uses: Many uses; Should not be heated Description: Heavy porcelain dish with a grinder. Watch Glass: Uses: To grind chemicals to a powder. Spatula: Description: Curved glass. Uses: May be used as a beaker cover; May be used in evaporating very small amounts of Description: Made of metal or porcelain. liquid. Uses: To transfer solid chemicals in weighing. Funnel: Triangular File: Description: Metal file with three cutting edges. Uses: To scratch glass or file. Rubber Connector: Description: Glass or plastic. Uses: To hold filter paper; May be used in pouring Description: Short length of tubing. Medicine Dropper: Uses: To connect parts of an apparatus. Pinch Clamp: Description: Glass tip with a rubber bulb. Uses: To transfer small amounts of liquid. Forceps: Description: Metal clamp with finger grips. Uses: To clamp a rubber connector. Test Tube Rack: Description: Metal Uses: To pick up or hold small objects. Beaker: Description: Rack; May be wood, metal, or plastic. Uses: To hold test tubes in an upright position. -
Corning Life Sciences Selection Guide Issue 6
www.corning.com/lifesciences Corning Life Sciences Selection Guide Issue 6 Trust Corning for Your Research For superior quality and performance in life science research tools, you can count on Corning. Our comprehensive line of advanced products and technologies is designed to meet your evolving needs in cell-based and genomics research. To learn more, please visit www.corning.com/lifesciences, or call 1.800.492.1110. Customers outside the U.S., please call 1.978.442.2200 or contact your local support office (listed below). Corning Incorporated Worldwide India Taiwan United Kingdom Life Sciences Support Offices t 91-124-235 7850 t 886 2-2716-0338 t 0800 376 8660 f 91-124-401 0207 f 886 2-2716-0339 f 0800 279 1117 Corning BV Life Sciences ASIA/PACIFIC Japan All Other European EUROPE Koolhovenlaan 12 Australia t 81 (0) 3-3586 1996/1997 Countries 1119 NE Schiphol Rijk t 61 2-9416-0492 f 81 (0) 3-3586 1291/1292 France t 31 (0) 20 659 60 51 The Netherlands f 61 2-9416-0493 Korea t 0800 916 882 f 31 (0) 20 659 76 73 f 0800 918 636 Corning Incorporated China t 82 2-796-9500 f 82 2-796-9300 Germany LATINAMERICA Life Sciences t 86 21-3222-4666 t 0800 101 1153 Brasil Tower 2, 4th Floor f 86 21-6288-1575 Singapore f 0800 101 2427 t (55-11) 3089-7419 900 Chelmsford St. Hong Kong t 65 6733-6511 The Netherlands f (55-11) 3167-0700 Lowell, MA 01851 t 852-2807-2723 f 65 6861-2913 Mexico t 800.492.1110 f 852-2807-2152 t 31 20 655 79 28 New! t (52-81) 8158-8400 t 978.442.2200 f 31 20 659 76 73 f (52-81) 8313-8589 f 978.442.2476 www.corning.com/lifesciences Online ordering now available 6/08 6M APC CLS–PSG–001-A4-REV6 Corning, Costar, CellBIND, CellCube, CellSTACK, DNA-BIND, Lambda, Octapette, Spin-X, Stripette, Stripwell, Thermowell, Transtar-96, Transwell,, 8-Pette, and 12-Pette are registered trademarks of Corning Incorporated, Corning, NY. -
Laboratory Manual
International Program UAM-Boston University Laboratory Manual Organic Chemistry I 2013-2014 Departamento de Química Orgánica Ernesto Brunet Romero Ana María Martín Castro Ramón Gómez Arrayás Laboratory Manual Table of Contents ............................................................................... 1 Introduction ............................................................................... 2 Prelab preparation ............................................................................... 2 Notebook ............................................................................. 3 Safety .............................................................................. 3 Laboratory Practices and Safety Rules ............................................................. 4 Accidents and injuries ........................................................................... 5 Fires ............................................................................. 5 Chemical Wastes ............................................................................. 6 Cleaning Responsibilities ............................................................................. 6 Lab cleanliness ............................................................................. 6 Laboratory Equipment ............................................................................. 7 Proper use of glassware ............................................................................. 8 Some techniques in lab experiments Heating, cooling and stirring ............................................................................ -
Chemistry Laboratory Safety Rules
Orange Coast College Chemistry Laboratory Safety Rules All chemistry students must successfully pass a test on the following material before they are allowed to work in the laboratory. The test consists of 20 multiple choice questions. The importance of safety requires a high passing score. General Rules 1. No eating, drinking, chewing gum, smoking, or vaping is permitted in the laboratory. 2. Shoes (closed-toed) and appropriate clothing must be worn in the laboratory. Sandals, shorts, etc. do not provide sufficient protection from an accident. To prevent damage, avoid wearing expensive clothing in the lab. 3. Be prepared for lab. Read the procedures carefully before your scheduled lab period and follow all instructions. 4. No unauthorized experiments or unsupervised laboratory work is permitted. An unauthorized experiment is anything that is not in the experimental procedures or instructions given by your professor. 5. Visitors and children are not allowed in the laboratory. Exit the lab if you need to communicate with your visitors. 6. Clean up all chemical spills immediately, including water on the floor. 7. Report any accident, no matter how minor, to the instructor. 8. Never leave your experiment unattended when there is a hazard such as a lit burner. 9. Before you leave the laboratory, always clean your work area, lock your drawer, make certain that water and gas are off, and counters and floor are clean and dry. Safety goggles are to be removed only immediately before leaving lab. 10. Keep backpacks & personal items on the coat rack or shelves above them, to keep the aisles free of tripping hazards. -
Isolation and Characterization of a Natural Product: Caffeine
Cerritos Community College Organic Chemistry 211 Laboratory Isolation and Characterization of a Natural Product: Caffeine. O O CH3 N H HN – H3C N O N N O N N P OH H2N N O O N N N N O H3C OH purine 2'-deoxyguanosine caffeine 5'-phosphate In this experiment, the techniques of solid-liquid extraction and liquid-liquid extraction will be used to isolate caffeine from tealeaves. A new purification technique, sublimation, will be used to perform the final purification. Caffeine is a member of the class of natural products called alkaloids, which contain nitrogen. Other members of this class include morphine, mescaline, and strychnine. The strong biological activity of these compounds has led to their thorough investigation by many different disciplines. Caffeine possesses the oxidized skeleton of purine, a relative of which can be found as a constituent of DNA (see above). Caffeine is found in many plants with coffee and tea being the most familiar. A cup of coffee or tea provides a 25- to 100-mg “Dose” of caffeine, which stimulates the central nervous system in a widely exploited manner. From the preceding, it is not surprising that caffeine is soluble in hot water, but it is also soluble in dichloromethane, allowing the following extractive protocol to isolate it. This two-step extractive isolation assures that ionic species remain in the aqueous layer while taking advantage of the swelling that water causes in the tea leaves. Simply extracting the tealeaves with dichloromethane directly is ineffective. The sodium carbonate added to the water during the “steeping” stage is to insure that the caffeine and organic acids are deprotonated. -
High School Chemistry
RECOMMENDED MINIMUM CORE INVENTORY TO SUPPORT STANDARDS-BASED INSTRUCTION HIGH SCHOOL GRADES SCIENCES High School Chemistry Quantity per Quantity per lab classroom/ Description group adjacent work area SAFETY EQUIPMENT 2 Acid storage cabinet (one reserved exclusively for nitric acid) 1 Chemical spill kit 1 Chemical storage reference book 5 Chemical waste containers (Categories: corrosives, flammables, oxidizers, air/water reactive, toxic) 1 Emergency shower 1 Eye wash station 1 Fire blanket 1 Fire extinguisher 1 First aid kit 1 Flammables cabinet 1 Fume hood 1/student Goggles 1 Goggles sanitizer (holds 36 pairs of goggles) 1/student Lab aprons COMPUTER ASSISTED LEARNING 1 Television or digital projector 1 VGA Adapters for various digital devices EQUIPMENT/SUPPLIES 1 box Aluminum foil 100 Assorted rubber stoppers 1 Balance, analytical (0.001g precision) 5 Balance, electronic or manual (0.01g precision) 1 pkg of 50 Balloons, latex 4 Beakers, 50 mL 4 Beakers, 100 mL 2 Beakers, 250 mL Developed by California Science Teachers Association to support the implementation of the California Next Generation Science Standards. Approved by the CSTA Board of Directors November 17, 2015. Quantity per Quantity per lab classroom/ Description group adjacent work area 2 Beakers, 400 or 600 mL 1 Beakers, 1000 mL 1 Beaker tongs 1 Bell jar 4 Bottle, carboy round, LDPE 10 L 4 Bottle, carboy round, LDPE 4 L 10 Bottle, narrow mouth, 1000 mL 20 Bottle, narrow mouth, 125 mL 20 Bottle, narrow mouth, 250 mL 20 Bottle, narrow mouth, 500 mL 10 Bottle, wide mouth, 125 -
Miniav®-X Automatic Viscometer Instruction & Operation Manual
MiniAV®-X Automatic Viscometer Instruction & Operation Manual 81.2254 i CONTENTS 1 INTRODUCTION/INSTALLATION 1 The miniAV®-X Automatic Viscometer .................................................................................. 1 Measuring kinematic viscosity ............................................................................................... 2 Safety cautions ..................................................................................................................... 2 Specifications ....................................................................................................................... 4 Installation ............................................................................................................................ 4 Required installation components ............................................................................... 4 Vacuum Pump unit connections ................................................................................. 6 Bath unit connections ................................................................................................ 6 VISCPRO® for Windows® XP® ............................................................................................ 6 Installing VISCPRO® software .............................................................................................. 7 Computer requirements ............................................................................................. 7 Windows® XP® installation .......................................................................................