International Conference on Education, Technology, and Science NETS 2013

Improving The Quality of Education to Face The Impact of Technology

PROCEEDINGS

Universitas Muhammadiyah Purwokerto Purwokerto, December 28th, 2013

i

Preface Rector of UMP Assalamu’alaikum wr. wb. Alhamdulillah, that UMP will carry out prestigious event namely “National Olympiad and International Conference on Education, Technology, and Science (NETS) 2013” is a very great news for me. NETS 2013 is the first event which is conducted in collaboration between UMP and PWM Central Java. It is a great event which is constructively supported by ITB, UNiSA, UTeM, and several institutions under UMP partnership to smoothen the event. Recently, technology grows very fast. This fast growth affects on the quality of education in Indonesia. Based on this condition, UMP and PWM Central Java formulate a program which later it is formed into “NETS 2013”. This event is also a contribution to Islamic Higher Education Institution to prepare candidate of future leaders who have competitive skills to compete positively in comprehending knowledge and technology. In NETS 2013, there are some events which are arranged in a sequence such as: International Conference on Education, Technology, and Science (ICETS) with Prof. Dr. Ir. Imam Robandi, M.T., Mr. Ganjar Pranowo, Mr. Simen Lourds, and Dr. Hiroshi Takeda; Seminar (Call for Paper) including some disciplines of science covering Education, Engineering, Medicine, Science, Social, Politic, Religion, Law, Culture, Arts, Agriculture, and Technology; National Olympiad for Student and Teacher (NOST) which is joined by students, teachers, and schools; and Expo. In NETS 2013 competitions, participants are fighting over trophies, medals, certificates, and advisory fund reaching up to Rp250.000.000,- ; furthermore, there is also a fellowship to study in UMP. As the Rector of The University of Muhammadiyah Purwokerto, I do really expect that conducting this event could encourage all of us to be more advanced nation not only on economic side, but also on social life. It is also expected that this event will increase our awareness on how important it is to develop and advance technology in education. That is all I could deliver now, for further information you may access our website on www.ump.ac.id. Last but not least, “Let’s join and support NETS 2013 in UMP”. Wassalamu’alaikum wr. wb. Purwokerto, 28th Decembe 2013

Rector,

Dr. H. Syamsuhadi Irsyad, M. H.

ii

Preface

The Chairman of NETS 2013

Assalamualaikum, wr. wb. Today is the valuable and precious milestones for education in Indonesia in general and Muhammadiyah in particular because all levels of education from elementary schools to university get together to build relationship and networking in the national Olympiad and international conference hosted by University of Muhammadiyah Purwokerto.

We deserve rejoice and pride because there are more than 1705 participants, 215 officials and 300 invited guests. This is also a great honour for University of Muhammadiyah Purwokerto to have the Governor of Central Java and the staffs, the regents from four regions and the staffs, the chief of Central Muhammadiyah Board , the Education division of Muhammadiyah Board, the directorate of higher education, Rectors of University of Muhammadiyah throughout Indonesia and rectors of universities in Purwokerto, companies and stake holders, media , travel biro as well as the colleagues, persistent fighters for Muhammadiyah in the region and territory.

As the chairman of this events, I‘d like to report that there are more than 1616 teachers and students who will be joining the national Olympiad and there are more than 311 researchers, lecturers and students will be taking part in the International Conference. Again, I’d like to emphasis that this event is really a pride for us because the participants are coming representing five big islands in Indonesia, they are Sumatra, Kalimantan or Borneo, Java and Sulawesi.

This event holds thirty one types of competitions and organizing six international conferences. There are twenty five competitions for students, four competitions for teachers and three competitions for schools. The international conference covers fields of Educations, Engineering, Science and Agriculture, Health and Medicine, Culture and Arts, social, Politics, Economics, Religion and Law.

We are proud to mention that we have at least fifteen experts coming from higher education, one from high schools, two industries and one national research centre (LIPI). The experts and scholars are coming from some outstanding universities and institutes such as University of South Australia, Tottori University Japan, Institute Teknologi Melaka, Malaysia, Henderson Secondary School Singapore, Boromanjani College of Nursing Thailand, Surabaya Technology

iii

Institute Bandung Technology Institute, Gadjah Mada University, Bogor Agriculture Institute, and University of Muhammadiyah Purwokerto. On behalf of the committee, we’d like to express our deep gratitude and thanks to all sides for their sincere helps and supports that make this event possible to happen in UMP. Last but not least, we have tried and done our best in organizing this event, however, we realized that weaknesses and shortcomings may exist. And for that particular reason we’d like to apologize to you all. Hopefully, the next year NETS will be much better and much improved. Have a great competition and conference!

Wassalamualaikum wr.wb. Purwokerto, 28th Decembe 2013

The Chairman Regawa Bayu Pamungkas, ST., MT.

iv

Organizing Committee

The Responsible : Dr. H. Syamsuhadi Irsyad, MH. (Rector of Universitas Muhammadiyah Purwokerto) Steering Committee : Prof. Dr. Ir. Imam Robandi, MT. (ITS) Prof. Dr. Din Syamsudin (PP Muhammadiyah) Prof. Dr. Baedowi (UNS) Dr. Syamsuhadi Irsyad, SH., MH. (Rektor UMP) Dr. Chairil Anwar (Dikti Litbang PP Muhammadiyah) Prof. Dr. DYP. Sugiharto (Koor.Kopertis Wilayah VI) Drs. Martono, M.Pd. (PWM Jawa Tengah) Prof. Dr. H.M. Daelamy SP. (PWM Jawa Tengah) Prof. Dr. Dodi Nandika (IPB) Prof. Dr. Abdullah (UNDIP) Prof. Dr. Masrukhi, M.Pd. (UNNES) Prof. Dr. Suparman Syukur (UIN WALISONGO) Dr. Ir. Danu Ariono (ITB) Prof. Dr. Djoko Wahyono, SU., Apt. (UGM) Prof. Dr. Rochadi Abdulhadi (LIPI) Prof. Dr. Kamaludin, M.Pd. (Staff Ahli Deputi Gubernur DKI) Prof. Dr. Imam Santoso, M.Si. (UNSOED) Prof. Dr. Mifedwil Jandra Mohd. Janan (UTM) Dr. Mariana binti Yusof (UTeM) Dr. Elvia Shauki (UNiSA)

Chair : Ir. Regawa Bayu Pamungkas, MT. Co-chair : Watemin, SP., MP.

Program : Anwar Ma’ruf, ST., MT. Santhy Hawanti, Ph.D. Juanita, ST., MT. Susanti, M.Phil., Apt. Agus Mulyadi, SP., MP. Khristanto, S.S., M.Hum. Naelati Tubastuvi, SE., M.Si.

Editor : Sularso, Amd. Nur Afifah Indriyani

v

Keynote and Invited Speakers

Keynote Speakers Prof. Imam Robandi (ITS, Indonesia) Mr. Simen Lourds (Singapore) Dr. Hiroshi Takeda (Tottori University, Japan)

Invited Speakers Elvia Shauki, Ph.D. (University of South Australia, Australia) Prof. Dr. Mifedwil Jandra Mohd. Janan (UTM, Malaysia) Panarut Wisawatapnimit (Boromarajonani College of Nursing, Bangkok, Thailand) Dr. Ir. Danu Ariono (ITB, Indonesia) Prof. Dr. Djoko Wahyono, SU., Apt. (UGM, Indonesia) Dr. Chairil Anwar (UGM, Indonesia) Prof. Dr. Rochadi Abdulhadi (LIPI) Prof. Dr. Sugeng Priyadi (UMP, Indonesia) Herman Soemantri, ST., M.Si. (PT. Pertamina)

vi

CONTENTS

Preface Rector UMP ii

Preface Chairman NETS 2013 iii

Organizing Committee v

Keynote and Invited Speakers vi

EDUCATION

THE IMPACT OF MULTI MEDIA TECHNOLOGY TO ENGLISH TEACHING 1-5 Jusmin H.J Wahid, Universitas Ahmad Dahlan

THERE IS AN ELEPHANT IN MY ROOM:CHALLENGES IN TEACHING MULTIPLE 6-12 LEVELSOF REPRESENTATION FOR CHEMISTRY PRESERVICE TEACHERS Muhamad Imaduddin, Muhammadiyah Semarang University

DEVELOPING A CHEMISTRY MAGAZINEAS A LEARNING RESOURCE OF YEAR-10 13-18 STUDENTS OF SENIOR HIGH SCHOOL Eko Yuliyanto, Muhammadiyah Semarang University

IMPROVING LEARNING OUTCOMES THROUGH THE APPLICATION OF 19-24 COLLABORATIVE LEARNING MODEL DIRECT AND COOPERATIVE TYPE STAD ON MACROECONOMIC THEORY COURSES Luqman Hakim, Universitas Negeri Surabaya

EFL TEACHERS AND DIGITAL TEACHING MEDIA 25-29 1Suwartono, 2Asfi Aniuranti, 1Muhammadiyah University of Purwokerto, 2Gajah Mada State University of Yogjakarta

DEVELOPING A COMPREHENSION BASED INTERACTIVE READING MODEL 30-37 Pudiyono, Muhammadiyah University of Purwokerto

DEVELOPMENT OF EXPERTISE COMPETENCY BASED PHYSICS PRACTICAL 38-42 WORK ON THE FIELD OF MASONRY AND CONCRETE CONSTRUCTION ENGINEERING IN VOCATIONAL HIGH SCHOOL Ristiana Dyah Purwandari, Student of Science Education Doctoral Program, Indonesia University of Education

INCREASING THE LEARNING OUTCOMES AND THE CRITICAL THINKING 43-47 THROUGH THE APPLICATION OF THE PROBLEM SOLVING APPROACH Riza Yonisa Kurniawan, Universitas Negeri Surabaya

THE LEARNING OF SPEAKING AND WRITING USING ICT ( INTERNET IN THE 48-51 CYBER CLASSROOM ) FOR THE STUDENTS OF SENIOR HIGH SCHOOL Sony, Post Graduate Program of English Education, Ahmad Dahlan University

FIVE WAYS TO PROMOTE CREATIVITY IN THE CLASSROOM 52-59 Ummu Rosyidah, Post Graduate Program of English Education, Ahmad Dahlan University

vii

THE VALUE EDUCATION LEARNING IN FORMING CHILDREN BEHAVIOR, AGE 5-6 60-63 YEARS OLD Rohimi Zamzam

INITIATING CULTURAL-BASED CHARACTER LEARNING IN PRIMARY SCHOOL 64-76 Masrukhi, Semarang State University

INFORMATION AND COMMUNICATION TECHNOLOGY IN TEACHING AND 77-83 LEARNING IN HIGHER EDUCATION: AN INCONCLUSIVE FINDINGS Meika Kurnia Puji Rahayu D.A., University of Muhammadiyah Yogyakarta, Charles Darwin University

TEACHING AND LEARNING ENGLISH THROUGH UNIFORM RESOURCE LOCATOR 84-87 (URL) STRATEGY Khamim Mustofa, SMP Muh 2 Kebumen

ANALOGICAL THINKING IN MATHEMATICS PROBLEM SOLVING 88-92 Irwani,Muhammadiyah University of Gresik

SPEECH ON THE BEGINNING OF CHILDREN AGES 5 YEARS 93-97 Perla Yualita, STIKes Aisyiyah Bandung

IMPROVING JUNIOR HIGH SCHOOL STUDENTS SCIENCE LITERACY BY USING 98-102 MULT IMEDIA ON INTEGRATED-SCIENCE LEARNING IN THEME OF TRASH AND ITS TACKLING EFFORT Dewi Nurdiyanti, Banu Kisworo, Tania Avianda Gusman, Chemistry Departement, Teacher Training and Education Faculty University of Muhammadiyah Cirebon

ENGINEERING

EFFECT OF TIME AND TEMPERATURE ON ULTRASOUND ASSISTED EXTRACTION 103-106 OF ANTIOXIDANT FROM Coleus tuberosus Albertus Adrian Sutanto, Yustian Suharto, and Hadiyanto, Chemical Engineering Department, Faculty of Engineering, Diponegoro University

WASTE WATER TREATMENT IN TOFU INDUSTRY BY USING MICROALGAE 107-110 Nannochloropsis Sp. Ali Makrup, Mutiara C and Widayat, Department of Chemical Engineering Diponegoro University

IMPROVING THE QUALITYOF BRIQUETTE FROM UTILIZATION OF SOLID WASTE 111-115 OF FURFURAL SYNTHESIS PROCESS AS AN EFFORTS IN PROVIDING ALTERNATIVE ENERGY RESOURCES Mitarlis and Tukiran, Department of Chemistry, Faculty of Mathematics and Natural Sciences State University of Surabaya

FUZZY LOGIC AS THE CONTROLLER IN ELECTRONIC LOAD CONTROLLER 116-119 SYSTEM M. Irfan, Machmud E., Nur Alif , M. Ilham P., Faculty of Engineering Department of Electrical Engineering Muhammadiyah University Malang

viii

AUDIT DATA PROCESSING TOOL TO SUPPORT ENERGY CONSERVATION 120-125 Galuh Prawestri Citra Handani, Rini Nur Hasanah, and Hadi Suyono, Electrical Engineering Department, Brawijaya University

FRACTIONAL DISTILLATION OF CLOVE OIL AT VACUUM PRESSURE 126-129 Widayat, Muhammad Hilman H, Lily Nurdiana,Hadiyanto, Ngadiwiyana*), Bambang Cahyono*) and Hantoro Satriadi Jurusan Teknik Kimia, Fakultas Teknik, Universitas Diponegoro, *)Jurusan Kimia Fakultas Sains dan Matematika Universitas Diponegoro

GOVERNOR MODEL USING FUZZY - PID CONTROLLER FOR LOAD FREQUENCY 130-133 CONTROL GENERATOR Akhmad Ramadhani 1) and Imam Robandi 2), Jurusan Teknik Elektro - FTI, Institut Teknologi Sepuluh Nopember Surabaya

DESIGN MODEL OF STATCOM USING PID CONTROLLER 134-135 Ario P H1), Hermansyah2), Farid D M3),Ratih M S4),Imam Robandi5), Institut Teknologi Sepuluh Nopember Surabaya

EXCITATION CONTROL SYSTEM DESIGN TO IMPROVE VOLTAGE STABILITYIN A 136-137 SINGLE MACHINE INFINITE BUS SYSTEM (SMIB) METHOD USING PID Hermansyah, Aji Akbar Firdaus, Ratih Mar’atus Sholihah, Farid Dwi Murdianto, Imam Robandi, Electrical Engineering Institute of Technology Sepuluh Nopember

FREQUENCY STABILITY IMPROVEMENT ON SINGLE MACHINE SYSTEM USING 138-141 FIRST ORDER POWER SYSTEM STABILIZER Nita Indriani Pertiwi1), Mei Adetya2), Andi Imran3), Abil Huda4),Imam Robandi 5) (1,2,3,5)Institut Teknologi Sepuluh Nopember, Surabaya, (4)UniversitasBorneo Tarakan, Kalimantan Utara

DESIGN AND DEVELOPMENT OF THE DC-TO-DC CONVERTER FOR HIGH POWER 142-146 LED Mochammad Rif´an, Nanang Sulistiyanto, Rahmadwati, Onny Setyawati, Electrical Engineering Department, Brawijaya University

SETTLING TIME CONTROL OF STEAM TURBINE POWER PLANT USING LINEAR 147-150 QUADRATIC REGULATOR (LQR) Ribka Stephani(1), Nita I. Pertiwi(2), Hidayatul Nurohmah(3), Hilmansyah(4) dan Imam Robandi(5) (1,2,4,5)Department of Electrical Engineering, Institut Teknologi Sepuluh Nopember (ITS) (3)Department of Electrical Engineering, Universitas Darul ‘Ulum Jombang

IMPLEMENTATION OF ELECTRONIC LOAD CONTROLLER TO IMPROVE THE 151-156 VOLTAGE QUALITY OF MICROHYDRO POWER PLANT AT BENDOSARI VILLAGE - PUJON - BATU OF EAST JAVA PROVINCE Rini Nur Hasanah, Teguh Utomo, and Nurussa’adah, Electrical Engineering Department, Brawijaya University

HYBRID POWER PLANT FEASIBILITY STUDY IN MANDANGIN ISLAND USING 157-162 HOMER SOFTWARE Soeprapto1, Mahfudz Shidiq2, Unggul Wibawa3, 1,2,3 Department of Electrical Engineering Faculty of Engineering, Brawijaya University, Malang

ix

APPLICATION OF COAL GASIFICATION AS SOURCE OF FUEL IN THE 163-166 CARBONATION PROCESS OF LOCAL KOKAS BRIQUETE Wijianto1, Subroto2), Dwi Aries Himawanto3) 1,2)Jurusan Teknik Mesin Fakultas Teknik Universitas Muhammadiyah Surakarta, 3)Jurusan Teknik Mesin Fakultas Teknik Universitas Sebelas Maret

GOVERNOR CONTROL MODEL DESIGN USING PID CONTROLLER IN STEAM 167-170 POWER PLANT Aji Akbar Firdaus1), Ribka Stephani2), Hidayatul Nurohmah3), Nita Indriani P.4), Imam Robandi5) 1,4,5) Department of Electrical Engineering, Institut Teknologi Sepuluh Nopember Surabaya, 2)Universitas Kristen Petra, Surabaya, 3)Universitas Darul Ulum, Jombang

DESIGN AND DEVELOPMENT OF VACUUM DISTILLERFOR BIOETHANOL 171-173 PRODUCTION Muhammad Aziz Muslim and Goegoes Dwi Nusantoro, Electrical Engineering Department, Brawijaya University

MODELING AND SIMULATION OF STEERBY WIRE CONTROL SYSTEM USING 174-177 FUZZY LOGIC Fachrudin1, Imam Robandi2, Nyoman Sutantra3 1Electrical Engineering Dept., Widyagama Univ., Malang, 2Electrical Engineering Dept., ITS Surabaya, 3Mechanical Engineering Dept., ITS Surabaya

EIGENVALUE STABILITY ANALYSIS BASED ON VALUE GENERATOR 178-179 EXCITATION SYSTEM MODEL ON SMIB Hilmansyah(1), Mei Aditya(2),, Rahmat(3) dan Imam Robandi(4) (1,2,3,4Jurusan Teknik Elektro, Institut Teknologi Sepuluh Nopember (ITS)

ANALYSIS OF BIDIRECTIONAL BUCK-BOOST CASCADE CONVERTER 180-181 PERFORMANCE WITH STATIC LOAD USING PSIM Muhammad Taufiq Ramadhan,Dedet Chandra Riawan,Imam Robandi, Department of Electrical Engineering, Faculty of Industrial Technology, ITS

COS IDENTIFICATION AND DESIGN OF WATER TURBINE CONTROL WITH LQR 182-186 METHOD Ratih Mar’atus S.(1),Aji Akbar F.(2),Ribka Stephani(3),Hidayatul Nurohmah(4),Imam Robandi(5) (1,2,5) Institute of Technology Sepuluh Nopember Surabaya (ITS), (3) Kristen Petra University, Surabaya, (4) Darul ‘Ulum University, Jombang

DESIGN OF POWER SYSTEM STABILIZER BASED FUZZY CONTROLLER WITH 187-191 STATE FEEDBECK FOR SINGLE MACHINE INFINITE BUS SYSTEM Tamaji, Imam Robandi, Department of Electrical Engineering, Faculty of Industrial Technology Sepuluh Nopember Institut of Technology

STABILITY ANALYSIS OF A POWER SYSTEM MODELING 2 AREA IN THE FORM OF 192-194 STATE SPACE Abil Huda, Riski C. A. H., Akhmad Ramdhani and Imam Robandi, Jurusan Teknik Elektro – FTI, Institut Teknologi Sepuluh Nopember Surabaya

x

ANALYSIS OF POWER SYSTEM STABILIZER (PSS) PERFORMANCE ON SINGLE 195-196 MACHINE INFINITE BUS (SMIB) USING PARTICLE SWARM OPTIMIZATION (PSO) ALGORITHM Hidayatul Nurohmah1), Nita Indriani Pertiwi2), Hilmansyah3), Andi Imran4), Imam Robandi5) 1),2),4),5)Department of Electrical Engineering, Faculty of Industrial Technology, ITS, 3)Politeknik Negeri Balikpapan

DESIGN AND DEVELOPMENT OF A BUILDING ENERGY AUDIT SOFTWARE 197-202 Rini Nur Hasanah, Hadi Suyono, Wijono, and Moch Dhofir, Electrical Engineering Department, Brawijaya University

DEVELOPMENT OF LIBRARY CODES FOR PROGRAMMINGTHE HUMANOID 203-207 ROBOTBASED ON CM-510 MODULE Muchlas and Ferry Yusmianto, Department of Electrical Engineering, Universitas Ahmad Dahlan

ANALYSIS OF MINIHYDRO POWER AND PHOTOVOLTAIC INJECTION INTO THE 208-212 GRID SYSTEM Hadi Suyono, Muhammad Fauzan E.P., Hari Santoso, Department of Electrical Engineering, Faculty of Engineering, Brawijaya University

EFFECT OF ETHANOL-96% IN GASOLINE WITH MIXTURE RATIO OF 1:9 AND 2:8 213-218 ON THE COMBUSTION AND EMISSION OF 125cc FOUR-STROKE ENGINE Dwi Fadila Kurniawan, Eko Siswanto, Erni Yudaningtyas and Eka Maulana, Electrical Engineering Department, Brawijaya University

A STUDY ON THE PERFORMANCE AND EXHAUST EMISSIONS OF A DI CI ENGINE 219-225 FUELLED WITH DIESEL/LPG BLENDS Munzir Qadri, Dept. of Mechanical Engineering, Faculty of Engineering, Universitas Muhammadiyah Jakarta

BUILDING AND DESIGNING CHEAP TELECOMMUNICATION SYSTEM FOR 226-231 TENAGA KERJA INDONESIA (TKI) Wahyu A. P., Teknik Elektro Fakultas Teknik Universitas Brawijaya

DC MOTOR SPEED CONTROL USING DYNAMIC LINEAR QUADRATIC REGULATOR 232-234 (LQR) Dwi Ajiatmo1,2 and Imam Robandi3 1)Department of Electrical Engineering, Faculty of Industrial Technology ITS, 2)Department of Electrical Engineering, Faculty Engineering of Darul ‘Ulum University Jombang, 3)Department of Electrical Engineering, Faculty of Industrial Technology ITS Surabaya

OPTIMIZATION OF PID CONTROLLER AND DUAL INPUT POWER SYSTEM 235-242 STABILIZER IN ELECTRICAL SYSTEM USING FIREFLY ALGORITHM Mhd Fanyand Imam Robandi, Department of Electrical Engineering, Faculty of Industrial Technology ITS Surabaya

PERFORMANCE ANALYSIS OF TIME DIVISION MULTIPLEXING (TDM)FOR 243-247 ANALOG SIGNAL THROUGH OPTICAL FIBERAND CONDUCTOR TRANSMISSION Sholeh Hadi Pramono, Sapriesty Nainy Sari, and M. Fauzan Edy Purnomo, Department of Electrical Engineering, Faculty of Engineering, Brawijaya University

xi

ORGANIC SOLAR CELL BASED ON EXTRACTION OF PAPAYA (Carica papaya) AND 248-251 JATROPHA (Ricinus communis) LEAVES IN DSSC (DYE SENSITIZED SOLAR CELL) Sholeh Hadi Pramono, Eka Maulana, M. Julius St., and Teguh Utomo, Department of Electrical Engineering, Brawijaya University

EFFECTS OF TIME AND TEMPERATURE OF POLY ETHER ETHER 252-254 KETONESULFONATION (sPEEK) ON THE SOLUBILITY OF sPEEK IN DIMETHYLACETAMIDE Muhammad Mujiburohman, Nur Hidayati, HerryPurnama, Siti R. Nihayati, Vendi Kurniawan, and Muhammad F. Hakim, Department of Chemical Engineering Universitas Muhammadiyah Surakarta

PSS - SMIB SIMULATION USING PSIM & MATLAB SOFTWARE 255-257 1)Andi Imran, 2)Hilmansyah, 3)Mei Adetya, 4)Yonny, 5)Imam Robandi 1,3,4,5)Electrical Engineering Department ITS, 2)Politeknik Balikpapan

RENEWABLE ENERGY DISTRIBUTED GENERATION AS A SMART SOLUTION FOR 258-262 THE ENERGY CRISIS IN INDONESIA Ramadoni Syahputra1,2, Imam Robandi and Mochamad Ashari2 1Department of Electrical Engineering, Faculty of Engineering, Universitas Muhammadiyah Yogyakarta, 2Department of Electrical Engineering, Faculty of Industrial Technology, Institut Teknologi Sepuluh Nopember

DESIGN OF DC VOLTAGE REGULATOR CONTROLLED BY BOOST CONVERTER 263-264 CLOSE LOOP SYSTEM WITH STATIC LOAD USING PSIM Abdil Jabbar Asyadili, Rodhi Kelvianto,Muhammad Taufiq Ramadhan,Imam Robandi, Department of Electrical Engineering, Faculty of Industrial Technology, ITS

IMPLEMENTATION OF ARITIFICIAL NEURAL NETWORK ALGORITHM AND 265-273 PREPROCESSING WITH SOBEL ALGORITHM Nurvelly Rosanti and Nera Kholimah, Informatic Engineering Course, Faculty of Engineering, University of Muhammadiyah Jakarta

PREDICTION OF TEST ITEMS VALIDITY USING ARTIFICIAL NEURAL NETWORK 274-279 Hindayati Mustafidah1), Sri Hartati2), Retantyo Wardoyo3), Agus Harjoko4) 1)Informatics Engineering-Muhammadiyah University of Purwokerto (Computer Science Doctoral Student-FMIPA-Gadjah Mada University), 2)3)4)Computer Science-FMIPA-Gadjah Mada University

PERFORMANCE ANALYSIS OF CLASSIFICATION ALGORITHM OF DATA MINING 280-285 TO STUDENTS STATUS IN HIGHER EDUCATION Imam Tahyudin, Azhari Shouni Barkah, Department of Information System, STMIK AMIKOM Purwokerto

FREQUENCY AND VOLTAGE CORRECTION IN SINGLE MACHINE SYSTEM USING 286-289 LINEAR QUADRATIC GAUSSIAN FEED BACK Farid Dwi M.(1),Ratih Mar'atus S.(2),Aji Akbar F.(3),Ribka Stephani(4),Imam Robandi(5) (1,3,5) Tenth of November Institute of Technology (ITS), (3) Christian of Petra University, Surabaya

xii

ANALYSIS CONTROLLABILITY, OBSERVABILITY, STABILITY IN SMIB BY 290-291 GOVERNOR Mei Adetya Praja, Abil Huda, Yoni Wicaksono, and Imam Robandi, Department of Electrical Engineering-Faculty of Industrial Technology,Institute ofTechnology Surabaya (ITS)

ANALYSIS OF MYCROHYDRO POWER PLANT SYSTEM USING COS 292-294 Riski Cahya Anugrerah Haebibi(1), Ario P.H(2), Akhmad Ramadhani(3),Hermansyah(4), Imam Robandi(5), Electrical Engineering Department-FTI, Institut Teknologi Sepuluh Nopember ITS

OLTC TRANSFORMER USING AVR WITH FUZZY LOGIC CONTROL 295-296 Yonny Wicaksono, Imam Robandi, Jurusan Teknik Elektro ITS, Surabaya

OPTIMAL FUZZY LOGIC CONTROLLER PADA PRESSURIZED WATER REACTOR 297-303 (PWR) PEMBANGKIT LISTRIK TENAGA NUKLIR MENGGUNAKAN CUCKOO SEARCH ALGORITHM Prima Dewi Permatasari1), Agus Dharma2), Imam Robandi3) 1,3) Jurusan Teknik Elektro, Fakultas Teknologi Industri, Institut Teknologi Sepuluh Nopember (ITS), 2) Jurusan Teknik Elektro, Fakultas Teknik, Universitas Udayana, Denpasar – Bali

MANAJEMEN ALIRAN DAYA PADA SISTEM PENGGERAK ELEKTRIK MOTOR 304-309 INDUKSI BERBASISKAN MULTILEVEL INVERTER JEMBATAN-H BERTINGKAT UNTUK APLIKASI MOBIL MATAHARI (SOLAR CAR) Muhamad Otong, Dedet Candra Riawan,Imam Robandi, Teknik Elektro-ITSSurabaya

HEALTH AND MEDICINE

TRADITIONAL AND NEW COMPLEMENTARY THERAPY OF NURSING 310-316 Panarut Wisawatapnimit, Boromarajonani College of Nursing, Bangkok, Thailand

EFFECTIVENESS OF ACUPRESSURE MASSAGE TOWARD CHANGE OF MOOD 317-320 DURING THE MENSTRUAL PERIODS Agustina Ari Handayani, Nursing Program of An Nur Health School, Purwodadi-Grobogan

DESCRIPTION OF KNOWLEDGE ABOUT BREAST FEEDING CONTRACEPTION 321-323 DURING LACTATION IN MIDWIFE CLINIC OF MRS. MINARTI PRINGAPUS VILLAGE PRINGAPUS DISTRICT SEMARANG REGENCY Rinta Nursanti, Eko Mardiyaningsih, Eka Adimayanti, Ngudi Waluyo Nursing Academy

THE DEVELOPMENT OF INTEGRATIVE MEASUREMENT MODEL OF PATIENT 324-327 SAFETY CLIMATE OF HOSPITAL IN DKI JAKARTA, 2013 1Rachmawati, Emma., Linda, Onny., Fikri. Ipik M., Prita Dewi, Evindiyah, 1University of Muhammadiyah Prof DR HAMKA (UHAMKA) Jakarta

THE MODEL OF GLUCOSE FLUCTUATION WITH DIABETES BASED ON MAPPING 328-331 HbA1c AND MEAN AMPLITUDO OF GLUCOSE EXCURSION VALUE Lailis Syafa’ah1, Rasad Indra2, Budi Santoso3, Askandar Tjokro Prawiro4 1,3Technique Faculty Electronic Department Muhammadiyah University Malang, 2,4Medical Faculty Brawijaya University THE EFFECT OF HIGH ENEMA TO DECREASE BLOOD UREUM LEVEL OF CHRONIC 332-335

xiii

RENAL FAILURE PATIENTS OF PROF. DR. MARGONO SOEKARDJO PUBLIC HOSPITAL PURWOKERTO Made Suandika

RELATIONSHIP BETWEEN HYPNOBIRTHING RELAXATION TECHNIQUES AND 336-338 CHILDBIRTH DURATION OF STAGE 1 ON BPM SAYIDAH KEC. KALIWUNGU KAB. KENDAL Maria Ulfah Kurnia Dewi

TELEHEALTH IMPLEMENTATION USING AN ONLINE MEETING APPLICATION FOR 339-342 THE REMOTE AREA HEALTH SERVICES Muchlas*and Rizky Nur Ardia Oktaviani** *Department of Electrical Engineering, Universitas Ahmad Dahlan, **Department of Public Health, Universitas Ahmad Dahlan

DEVELOPMENT OF SURV-TB (TUBERCULOSIS SURVEILLANCE WEBGIS) 343-346 Noor Alis Setiyadi1, Sabarinah B. Prasetyo2 1Prodi Kesehatan Masyarakat, Fakultas Ilmu Kesehatan, Universitas Muhammadiyah Surakarta, 2Fakultas Kesehatan Masyarakat, Universitas Indonesia

STUDY OF POST-STROKE HYPERGLYCEMIA THERAPY ON STROKE INPATIENTS 347-350 IN PKU MUHAMMADIYAH YOGYAKARTA HOSPITAL Nurul Maziyyah1) Sugiyanto2) Inayati3) 1)Pharmacy Programme, UMY, 2)Faculty of Pharmacy, UGM, 3)PKU Muhammadiyah Yogyakarta Hospital

WEI CHI 14 MERIDIANS EXERCISE AND PATIENT AFFIRMACY AS THE 351-354 EMOTIONAL THERAPY Sutarno*, Widyoningsih** *Sutarno: Graduate Nursing, Health Science School Al-Irsyad Al-Islamiyyah Cilacap

THE EFFICIENCY OF GARLIC AND CUCUMBER CONSUMPTION IN DECREASING 355-360 THE BLOOD PRESSURE AMONG THE ELDERS WITH HYPERTENSION Priyo,Prita Oktafiyana, Faculty of Health Sciences, Magelang Muhammadiyah University

IS THERE A DIFFERENCE BETWEEN FISAL IN STANDARDIZED EXTRACT FROM 361-366 PHYSALIS ANGULATA. L AND CONTROL ON PANCREATIC FUNCTION OF SPRAGUE DAWLEY RAT INDUCED BY STREPTOZOTOCIN-NICOTINAMIDE? Yeny Sulistyowati, Sri Kadarsih Soedjono, Mustofa, Budi Mulyono

PHENOMENOLOGY STUDY OF HELPLESSNESS ELDERLY EXPERIENCE WITH 367-372 HYPERTENSION IN BOGOR CITY Abdul Wakhid, Achir Yani S. Hamid, and Yossie Susanti Eka Putri

PROFILE OF JOB SATISFACTION AND ITS EFFECT ON CARING BEHAVIOR OF 373-377 HOSPITAL NURSES Jebul Suroso, Faculty of Health Science of Muhammadiyah University of Purwokerto

xiv

HOW TO INCREASE MILK PRODUCTION FROM MOTHER WITH INFANT LOW 378-382 BIRTH WEIGHT (LBW) Umi Solikhah 1, Indri Wisudawati Anggraeni2, Sodikin3 1,3Faculty of Health Science University of Muhammadiyah Purwokerto, 2Nurse

CORRELATION BETWEEN SOCIAL SUPPORT AND POSTPARTUM QUALITY OF 383-388 LIFE Faizah Betty Rahayuningsih, Health Science Faculty, Universitas Muhammadiyah Surakarta

CORRELATION BETWEEN KEGEL EXERCISE WITH NEEDLE PERINEUM INJURY 389-392 HEALING AT NORMAL POSTPARTUM WOMAN IN RB AFIATUN HASANAH BANDUNG Annisa Ridlayanti, STIKes Aisyiyah Bandung

THE CORRELATION BETWEEN SELF-CONFIDENCE AND DEPRESSION LEVEL OF 393-395 TEENAGE ORPHANAGES IN PANTI ASUHAN YAYASAN SATU UMAT IN MAGELANG Peni Nurman, Sambodo Sriadi Pinilih, Priyo, Nursing Science Department ofHealth Science Faculty of Muhammadiyah University of Magelang

DETERMINATION AND VALIDATION METHOD OF TOTAL FLAVONOID CONTENT 396-405 AND TOTAL PHENOLIC CONTENT OF ETHANOLIC EXTRACT OF MANGOSTEEN (Garcinia mangostanaL.) LEAVES AS NATURAL PRESERVATIVES CANDIDATE BY USING SPECTROPHOTOMETRIC METHOD Diniatik, Indri Hapsari, Milani Tiara, Ayu Meidyawati, Suhri Nurhidayat, Faculty of Pharmacy Muhammadiyah University of Purwokerto

FORMULATION AND ANTIOXIDANT ACTIVITY TEST OF LIPSTICK FROM 406-411 MANGOSTEEN RIND (Garcinia mangostana L.) METHANOL EXTRACT Gigih Aditya Pamungkas*, Nuryanti, Iskandar Sobri, Departement of Pharmacy FKIK Jenderal Soedirman University

THE ROLE OF MEDIA EXPOSURE AND HEALTH BELIEF ON BREAST SELF 412-415 EXAMINATION Khamidah Achyar,1 Anita D. Anwar,2 H. Herry Garna2 1University Muhammadiyah Purwokerto, 2 Padjadjaran University

PHENOMENOLOGY STUDY OF VARIOUS OBSTACLE EFFORTS TO ELIMINATEOF 416-420 DENGUE HAEMORAGIC FEVER IN BANYUMAS DISTRICT Isna Hikmawati, Dedy Purwito

RELATIONSHIP BETWEEN ANTENATAL CARE SERVICES AND PERINATAL 421-424 MORTALITY RISK FACTORS IN PUBLIC HEALTH CENTER OF PALEMBANG IN 2012 Rosmiarti,Enderiasari, Midwifery Diploma Degree Muhammadiyah Palembang

EFFECT OF MIND BODY COMPLEMENTARY THERAPIES: LISTENING TO 425-429 PASSAGESOF ALQUR’AN AGAINST LOWERING BLOOD PRESSURE AT SOCIAL INSTITUTION OF TERATAI NURSING HOMES PALEMBANG2013 Trilia, Nursing of Science Diploma Degree Muhammadiyah Palembang

xv

EFFECT OF DHIKR THERAPY ON LABOR PAIN KALA 1 AT MATERNITY CLINIC 430-436 BUDI INDAH IN PALEMBANG IN 2012 Maya Fadlilah and Mar’atun Ulaa, Nursing of Science Diploma Degree Muhammadiyah Palembang

ETHANOL EXTRACT OF BINAHONG LEAVES EFFECT (Anredera cordifolia (Ten.) 437-439 Steen) ON HEMATOLOGYCAL CHANGES IN FEMALE WISTAR RATS Silma Kaffah1, Fitri Kurniwati1, Anjar Mahardian Kusuma1, Asmiyenti Djaliasrin Djalil2 1Laboratory of Pharmacology-Toxicology, Faculty of Pharmacy, University of Muhammadiyah Purwokerto, 2Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Muhammadiyah Purwokerto

THE COMPARISON OF FREE RADICALS SCAVENGING ABILITIES BETWEEN 440-446 ETHANOL EXTRACTS OF PURWOCENG (Pimpinella pruatjan Molk.) HERBS AND LEAVES Balqis Hisyam Saleh Basleman, Wiranti Sri Rahayu, Retno Wahyuningrum, Pharmacy Faculty of Muhammadiyah University of Purwokerto

DIFFERENCES INFLUENCE PLYOMETRIC EXERCISE WITH NEUROMUSCULAR 447-454 ELECTRICAL STIMULATION (NMES) TO INCREASE THE ABILITY OF VERTICAL JUMP IN BASKETBALL PLAYER STIKES MUHAMMADIYAH PALEMBANG Putri Edrivania, Usman, Ika Guslanda Bustam, Program Study DIII Physical Therapy, STIKes Muhammadiyah Palembang

THE CORELATION BETWEEN KNOWLEDGE OF ANEMIA WITH ANEMIA INCIDENT 455-460 IN PREGNANTS Beti Rahayu Utami, Wilis Dwi Pangesti

THE IDENTIFICATION BACTERIA Streptococcus Pneumoniaee IN THE AIR ROOM 461-464 PAUD IN SEBERANG ULU 1 PALEMBANG Heri Shatriadi, Zairinayati, Hoetary Tirta Amalia, Environmental Health of Science Diploma Degree Muhammadiyah Palembang

THE DIFFERENCE ACCURACY APPRAISAL WEIGHT EMBRYOLOGY IN RISANTO 465-473 METHOD AND USG WITH WEIGHT BABIES WERE BORN IN A HEALTH CLINIC TEGALREJO 2009 Marlin, Riska, Science Diploma Degree Muhammadiyah Palembang

THE INFLUENCE OF EARLY BREASTFEEDING INITIATION TO THE DURATION OF 474-478 LABOR THIRD PERIOD IN MATERNITY MOTHER AT AL-JARIYAH MATERNITY HOSPITAL PALEMBANG IN 2013 R.A. Aminah Maya, Citra Purwanti, Prodi DIII Kebidanan STIKes Muhammadiyah Palembang

CYTOTOXIC ACTIVITY TEST OF EXTRACT AND FRACTION OF GALANGAL (Alpinia 479-487 galanga) ON HeLa CELLS BY INVITRO Puji Setya Rini, Irsan Saleh, Theodorus, Salni, Stikes Muhammadiyah Palembang

TOXIC EFFECTS OF FORMALDEHYDE KIDNEY Mice (Mus musculus L) SWISS 488-493 WEBSTER MALE Ra. Hoetary Tirta Amallia, Sri Nita, Theodorus Program Studi D III Kesehatan Lingkungan, STIKES Muhammadiyah Palembang, Indonesia

xvi

SCIENCE AND AGRICULTURE

FARMERS BEHAVIOR TOWARD RISKS OF POTATO FARMING FOR GRANOLA AND 494-497 ATLANTIC VARIETIES IN BANJARNEGARA REGENCY Pujiharto, Agricultural Faculty University of Muhammadiyah Purwokerto

THE EFFECTIVENESS OF CLOVE OIL TO CONTROL SOYBEAN RUST DISEASE 498-500 Sumartini and Eriyanto Yusnawan, Indonesian Legumes and Tuber Crops Research Institute

VEGETATIVE AND GENERATIVE PERFORMANCES OF THREE SOYBEAN 501-504 VARIETIES IN FIVE LEVELS OF COLCHICINE DOSAGES AND SEED COAT PEELING TREATMENTS Sutrisno and Heru Kuswantoro, Indonesian Legume and Tuber Crops Research Institute, Indonesian Agency for Agriculture Research and Development

SIMPLE PARTISIPATORY GIS FOR LAHAR HAZARD IDENTIFICATION IN CIMUJA 505-508 RIVER SUBANG DISTRICT Rendi Firdian I, Aditya Saputra, and Priyono, Geography Faculty Universitas Muhammadiyah Surakarta

THE ANALYSIS OF KALIUM -40 RADIONUCLIDE POLLUTION AT SEDIMENT, SEA 509-512 WATER AND SHRIMP (Penaeus Merguiensis) IN CILACAP COASTAL OCEAN Wahikun, Akademi Maritim Nusantara

THE EFFECT OF RHIZOBIUM INOCULATION ON TOTAL FLAVONOID, PHENOLIC 513-517 CONTENTS, AND ANTIOXIDANT ACTIVITY OF SOYBEAN SEEDS Eriyanto Yusnawan, Indonesian Legumes and Tuber Crops Research Institute

SCREENING OF MUNGBEAN MUTANT GENOTYPES RESISTANT TO POWDERY 518-521 MILDEW (Erysiphe polygoni) AND LEAF SPOT DISEASE (Cercospora canescens) Sumartini 1),Yuliastuti 2), Eriyanto Yusnawan 1) 1)Indonesian Legumes and Tuber Crops Research Institute, 2)Indonesian Atomic Institute

FUNCTION OF AGRICULTURE TECHNOLOGY ENVIRONMENT IN CARING FOR 522-525 FOOD SECURITY SUPPORT IN SUBDISTRICT BELIK OF PEMALANG REGENCY Watemin and Sulistyani Budiningsih, Agriculture Faculty of Muhammadiyah Purwokerto University

EFFECT OF RICE BRAN AND ANORGANIC FERTILIZER DOSAGESON YIELD OF 526-533 WHITE OYSTER MUSHROOM (Pleurotus ostreatus) Agus Mulyadi Purnawanto, Oetami Dwi Hajoeningtijas, Pujiati Utami, Faculty of Agriculture, University of Muhammadiyah Purwokerto

UTILIZATION OF WASTE OIL PALM AS A TOTAL MIXED RATION SILAGE WITH 534-537 LOCAL RESAURCES BASED TO ACHIEVED FOOD SECURITY IN AN ANIMAL PROTEIN SOURCES Agung Irawan, Mohammad Sholikin, Department of Animal Science, University of Muhammadiyah Malang

CALCULATION PENSION PLAN USING BENEFIT PRORATE METHOD (A Case Study 538-542 of State Lecturers and Employees at Muhammadiyah Cirebon University) Setyo Wira Rizki and Rifqi Hidayat

xvii

PROFILE OF MICROENTERPRISES CREATIVE SOUVENIR PROCESSED COCONUT 543-547 WASTE MANAGED BY POOR ARTISANS FARMERS IN RURAL Dumasari1, Tri Septin Muji Rahayu2, Sulistyani Budiningsih3 1,3Lecturer of Agribusiness Program Study, Faculty of Agriculture, University of Muhammadiyah Purwokerto, 2Lecturer of management Program Study, Faculty of Economic, University of Muhammadiyah Purwokerto

DEVELOPMENT OF FUSARIUM DISEASE CONTROL TECHNOLOGY WITH 548-555 BIOLOGICAL AGENT IN MAS CULTIVAR BANANA SEEDLINGS RESULT IN VITRO CULTURES Anis Shofiyani and Gayuh Prasetyobudi

SOCIAL ECONOMIC

SERVICES CAPACITY OF ISLAMIC FINANCIAL SERVICES COOPERATIVE IN THE 556-562 DEVELOPMENT OF SMALL AND MEDIUM ENTERPRISES (SMEs) INPURWOREJO CENTRAL JAVA INDONESIA (Analysis of Services Quality Effect to Loyalty with Satisfaction as an intervening variable in Business Partner of Islamic Financial Services Cooperative In District Purworejo) Agus Fitri Yanto, Danis Imam Bachtiar and Agus Dwi Atmoko, Polytechnic Sawunggalih Aji - Purworejo

THE ROLE OF SMALL AND MEDIUM ENTERPRISES (SMEs) TO 563-566 COMMUNITY BUSINESS DEVELOPMENT IN INDONESIA (A Study of Productive Economic Activities in Kalimantan) R. Deni Muhammad Danial1 and Heinrich Gultom2 1Student of Doctoral Program of Business Administration UNPAD, Lecturer of Business Administration, Muhammadiyah University of Sukabumi (UMMI), 2PNPM Support Facility- World Bank

CORRELATION BETWEEN INTENSITY OF PLAYING ONLINE GAME AND 567-571 EMOTIONAL INTELLIGENCE Ulfi Kholidiyah dan Susatyo Yuwono, Psychology Department of Muhammadiyah University of Surakarta

GAIN AND LOSS OF COMPARATIVE ADVANTAGE AMONG ASEAN-6 572-581 Yuli Utami, International Program for Islamic Economics and Finance, Department of Economics-University Muhammadiyah Yogyakarta

GAMES ONLINE INFLUENCE AT THE CAFE ON LANGUAGE DEVELOPMENT IN 582-585 CHILDREN AT KELURAHAN CEMPAKA PUTIH, CIPUTAT TIMUR, TANGERANG SELATAN Ati Kusmawati, Muhammadiyah Universityof Jakarta

ANALYSIS OF EFFECT OF PRICE, LOCATION AND SERVICES TO INCREASE SALES 586-588 VOLUME Henny Dwijayani, Departement of Management, Faculty Economics of Universitas Darul Ulum, Jombang

xviii

STUDY OF SELF-EFFICACY IN GRADUATE UNEMPLOYMENT 589-593 Suwarti and Ugung Dwi Ario Wibowo, Psychology Faculty of University of Muhammadiyah Purwokerto

EXISTENCE OF RURAL COMMUNITY IN THE DISSEMINATION OF AQUAPONIC 594-599 TECHNOLOGY AT AGROTURISM VILLAGE Imam Santosa1, Muslihudin2, Taufik Budhi Pramono3 1,2Sociology Study Program, Faculty of Social and Political Sciences, Jenderal Soedirman University, Purwokerto, 3Lecturer of Fishery Study Program, Faculty of Science and Engineering, Jenderal Soedirman University Purwokerto

THE ANALYSIS OF RISK AND RETURN ON THE PROFIT-LOSS SHARING PRODUCTS 500-606 IN THE INDONESIAN ISLAMIC BANKING INSTITUTION Yohani1, Achmad Tohirin2, Haryadi3, Sobrotul Imtikhanah4 1,4Lecturer at the Department of Accounting, Muhammadiyah College of Economics (STIE) of Pekalongan, 2Lecturer of Islamic Finance & Economics, Post Graduate Program, Faculty of Economics, Universitas Islam Indonesia, Yogyakarta, 3Lecturer at the Department of Management, Faculty of Economics, Universitas Jenderal Soedirman (UNSOED), Purwokerto

xix

Proceedings of International Conference On Education, Technology and Science (NETS) 2013 Purwokerto, December 28th, 2013

THE COMPARISON OF FREE RADICALS SCAVENGING ABILITIES BETWEEN ETHANOL EXTRACTS OF PURWOCENG (Pimpinella pruatjan Molk.) HERBS AND LEAVES

Balqis Hisyam Saleh Basleman, Wiranti Sri Rahayu, Retno Wahyuningrum Pharmacy Faculty of Muhammadiyah University of Purwokerto

Abstract has been widely reported have medicinal properties Flavonoids and tannins are potentially as free as aphrodisiac, diuretic, and tonic. This plant is radicals scavenger. Purwoceng has some of the native plant from Indonesia which live in endemic compounds such as flavonoid, glycosides, and areas, such as mountainous Dieng plateau in tannins. Research done by using DPPH and Nitric Central Java, mount Pangrango, West Java and East oxide (NO•) radicals. The treatment of ethanol Java’s mountainous areas. extracts of purwoceng leaves with the scavenging According to Caropeboka and Lubis (1975), activity of radical DPPH 38,65 ppm and NO• 14,73 root extract of purwoceng motor activity, which ppm results IC30 smaller when compared with the heightens tonus striated muscles, stimulates central ethanol extract of purwoceng herbs by the nervous arrangement with capture point work on scavenging activity of radical DPPH 57,25 ppm the medulla oblongata, sexual behavior and to and NO• 28,88 ppm. The free radicals scavenging improve male mice (Caropeboka et al., 1979 in S. abilities of ethanol extracts of purwoceng leaves Usmiati and S. Yuliana, 2010). So it is said that better than free radicals scavenging abilities of purwoceng’s root extract has an androgenic activity ethanol extracts of purwoceng herbs. in mice. Saponin group is the one of chemical Keywords: free radicals, Purwoceng, DPPH, nitric compound as secondary metabolites in purwoceng oxide plant. There are also triterpenoidsteroids, sitosterol, and stigmasterol compounds. Purwoceng INTRODUCTION plant also contains coumarin, alkaloids, flavonoids, Free radicals is an atom or group of atoms that glycosides, and tannins. By looking at the content has a highly reactive characteristic caused by the of secondary metabolites of purwoceng plant which presence of one or more free electrons or electrons are flavonoids and tannins (Haryono, 2013), is are not paired, so that it becomes unstable and high needful to do more researchs to find out the energy (Fessenden and Fessenden, 1982). To avoid potential of the power of free radicals scavenging of the free radicals attack, is by consuming natural purwoceng plant either the leaves or herbs because antioxidants in sufficient amount each day. Anti for specific flavonoid compounds are concentrated free radical is a compound which in a small amount in the leaves (the herb Information, 2012; Waji et when compared with the substratum is capable of al, 2009). delaying or preventing or avoiding the oxidation of substrates which are easily oxidized (Halliwell and RESEARCH METHODS Gutteridge, 2000). Tool Flavonoids are natural phenolic compounds The tools used in this research include: glass are potentially as free radicals scavenger and have tools (measuring flask, measuring cup, test tubes, bioactivities as a medication. Generally, leaf, root, erlenmeyer flask), micro pipette, Ultrasonic bath, stem and flower are the part of plant that save many Rotary Evaporator, analytic scales, maceration, flavonoids as secondary metabolites (Waji et al, cupboard dryer, exicator, UVVis 2009). Another natural phenolic compounds that Spectrophotometer. potential as a free radicals scavenger is tannin. Tannins can be found in all parts of the plant, but at Materials some plants, tannin concentrated on specific parts 1. Material Test (Hernawan and Setyawan, 2003). One of the plants Ethanol extracts of purwoceng herbs and that contain flavonoids and tannins as secondary ethanol extracts leaves (Pimpinella pruatjan metabolites is purwoceng (Haryono, 2013). Molk.). Purwoceng (Pimpinella pruatjan Molk.) is a medicinal plant. Commercially, purwoceng’s root

Universitas Muhammadiyah Purwokerto HM 440 ISBN 9786021493007 Proceedings of International Conference On Education, Technology and Science 2013 Purwokerto, December 28th, 2013

2. Chemicals 3) Preparation of Ethanol Extract of Ethyl acetate technical degrees, petroleum Purwoceng ether technical degrees, ethanol 96% technical Ethanol extracts of purwoceng leaves degrees, ethanol p.a., DPPH, sodium nitroprusside, and ethanol extracts of purwoceng herbs sulfanilic acid p.a., glacial acetic acid p.a., N(1 dissolved in methanol p.a. with a Naphthyl)ethylenediamine dihydrochloride, Griess concentration of 0.01% w/v. reagent p.a, BHT (butilatedhydroxytoluene), 4) Test Power Free Radicals Scavenging quercetin. As many as 1000 L DPPH 0.4 mM a. Preparation of Test Materials put in pumpkin measures, added by the test 1) Preparation of simplicia solution with different volume series (500, Cultivation purwoceng plants is 1000, 1500, 2000 and 2500 L) is then obtained from Dieng’s farmer, Wonosobo, added methanol p.a. up to 5.0 mL volume, Central Java. Purwoceng plants that have vortex for 1 minute until the mixture is been obtained are cleaned from impurities homogenous. After 30 minutes, the attached to later washed until clean. The absorbance is measure at a maximum plant had been dried with dryer cupboard. wavelength. Measure the negative control Further divided two groups that is group absorbance also without the addition of the with the herbs and group with the leaves test solution. only. Then powdered and sifted with fine The magnitude of resource free radicals powder 20 degrees so that the obtained scavenging is calculated using the formula: powders with a uniform size (anonymous, Resource free radicals scavenging 1979). Abs. Control Abs. Sample % = x 100% 2) Extraction of simplicia Abs. Control The dry simplicia powder was added to the container maceration. Donewith use 3 The acquired absorbance data was concentrate solution of maceration, namely created a linear regression equation which petroleum ether (PE), ethyl acetate (EA) and expressing the relationship between the ethanol 96%. Each maceration with a concentration of the test substance (x) with different concentrate performed as many as the average antioxidant activity (y) of a 3x24 hours in order to obtain optimal results series of measurements of replication so maceration. During submersion, stirring for retrieved IC50 value, is the test solution that 1 hour was done so that the compounds necessary scavenge 50% of DPPH radical contained in the simpilisia of purwoceng over the past 30 minutes (operating time), or leaves and herbs can dissolve properly. pause time required by the test solution to reduce the radical DPPH perfectly. After 30 b. Determining Free Radicals Scavenging minutes the absorbance obtained will be Quantitatively with DPPH Radicals constant. 1) Preparation of DPPH solution 0,4 mM DPPH solution of 0.4 mM is made by c. Determining Free Radicals Scavenging dissolving 15.8 mg powder DPPH in Quantitatively with Nitric Oxide Radicals methanol p.a. on pumpkin measures 100.0 (NO•) mL, then vortex. 1) Making a solution of Sodium 2) Determination of Maximum Wavelength Nitroprusside 10 mM of DPPH Solution Dissolve 299,12 mg of the powder of Determination of the maximum sodium nitroprusside in methanol p.a. on wavelength of DPPH 0,4 mM are used in the pumpkin measures 100,0/250 mL, then power of free radicals scavenging test is divorteks. done as follows: 1000 L DPPH solution 0,4 2) Griess Reagent Creation mm added to the volume with methanol 5 By dissolving 1,0 mL of reagent 0.33% mL then observed the absorbance in the sulfanilat acid in glacial acetic acid (20%), range of wavelength 400800 nm using then incubated for 5 minutes at room methanol p.a. as blank. temperature. Then add 1.0 mL of N(1 Naphthyl) ethylenediamine dihydrochloride 0.1% (w/v).

ISBN 9786021493007 HM 441 Universitas Muhammadiyah Purwokerto Proceedings of International Conference On Education, Technology and Science (NETS) 2013 Purwokerto, December 28th, 2013

3) Determination of Maximum Wavelength 2) Quercetin Stock solution 100 ppm Sodium Nitroprusid 10 mM The stock solution is made by Determination of the maximum weighing carefully 0,01 grams of quercetin wavelength of Sodium Nitroprusside 10 mM and dissolved in 100 mL of methanol p.a. used in the power of free radicals 3) Test Power Free Radicals Scavenging scavenging test is done as follows: 2000 L The power free radicals scavenging test sodium nitroprusside solution 10 mM added for a positive control is carried out with the with 500 mL of 7.4 pH phosphate buffer and power free radical scavenging test of ethanol proceed with incubation at a temperature of extract of herbs ethanol extract leaves 25°C for 150 minutes, then added with 500 purwoceng, but the series volume control L Griess reagent and measured the that is added is different. For BHT the absorbance in the range of wavelength 400 volume series are 25, 50, 100, 200 and 400 800 nm using methanol p.a. as blank. L of a stock solution of 100 ppm and to 4) Determination of Operating Time of measure the absorbance was used methanol Coupling Compound as blank, for quercetin the volume series are Determination of operating time of 2, 4, 8, 16 and 32 L of a stock solution of coupling compound was done by measuring 100 ppm and to measure the absorbance was the mixture of 2000 L sodium nitroprusside used same concentration of quercetin on test 10 mM and 500 L phosphate buffer (pH resource free radicals scavenging as blank. 7.4) and the Griess reagent. Then measure the operating time mixture solution at a RESULT AND DISCUSSION maximum wavelength by using methanol 1. Determination of Purwoceng Plant p.a. as blank. The results of determination for purwoceng 5) Preparation of Ethanol Extract of plant which is done according to the book the Flora Purwoceng of Java (Backer and Bakhuizen, 1965) is retrieved Ethanol extracts of purwoceng leaves the key determination stating the plant used is and ethanol extracts of purwoceng herbs Pimpinella pruatjan Molkenb. with synonym dissolved in methanol p.a. with a Pimpinella alpina Molk. concentration of 0.01% w/v. 6) Test Power Free Radicals Scavenging 2. Extraction of Purwoceng Plant 2.0 mL solution of sodium a. The results of the extraction of Herbs and nitroprusside 10 mM added with 500 L the leaves of Purwoceng phosphate buffer (pH 7.4) and added a test 10 kilograms of purwoceng plants are solution with different volume series (500, washed clean, drained and dried to constant 1000, 1500, 2000 and 2500 L) then added weight is obtained simplicia purwoceng methanol p.a. up to 5.0 mL volume, amounting to 1.242,88 grams so that simplicia followed by incubation at a temperature of drying shrinkage can be calculated which of 25°C for 150 minutes. Added by 500 L 11,66%. Then separated the leaves and herbs Griess reagent and incubated at room part and powdered. Obtained purwoceng herbs temperatures during the operating time. powder weights was 340 grams while for Read absorbance at a maximum wavelength. purwoceng leaves was obtained 550 grams. The dry powder extracted by maceration Decker d. Preparation of Positive Control Solution uses 3 different polarity concentrate. Ethanol 1) BHT Stock Solution 100 ppm 96% extract was concentrated until obtain the The stock solution is made by viscous extract of purwoceng herbs as much as weighing carefully 0,01 grams of BHT and 24,83 grams and viscous extract purwoceng dissolved in 100 mL of methanol p.a. leaves as much as 23,52 grams.

Universitas Muhammadiyah Purwokerto HM 442 ISBN 9786021493007 Proceedings of International Conference On Education, Technology and Science 2013 Purwokerto, December 28th, 2013

b. Extract Characteristics

Table 1. Purwoceng Viscous Extract Characteristics Viscous Extract Extract Characteristics Result Purwoceng Herbs 1. Organoleptic a. Color Dark green soupy b. Smell Typical c. Flavor d. Shape Viscous solution 2. Rendemen 7,33% Purwoceng Leaves 1. Organoleptic a. Color Dark green soupy b. Smell Typical c. Flavor d. Shape Viscous solution 2. Rendemen 23,52%

3. Determination of Resource Free Radicals Scavenging Quantitatively a. Results of The Determination of Maximum Wavelength (λ max) Results of maximum wavelength determination with the DPPH radical is 516 nm with maximum absorbance for an ethanol extract of purwoceng leaves is 0,756, ethanol extract of purwoceng herbs is 0,752, quercetin is 1,005 and BHT is 0,756. This means that for power of free radicals scavenging test performed on maximum λ 516 nm. Capture 2. Scanning (λ max) Sodium Nitroprusside Solution 10mM

b. Power Test Results of FreeRradicals Scavenging Power test results capture free radicals can be seen from the percent inhibition of free radical DPPH and NO• ethanol extracts of leaves and ethanol extracts of herbs of purwoceng, quercetin and BHT which shown in tables II, III, IV and V. Where the higher concentration of the extract is used so the Capture 1. Scanning (λ max) DPPH Solution percent inhibition is also higher which means 0,4mM the better the free radicals scavenging power.

Results of The Determination of Maximum Wavelength (λ max) Results of maximum wavelength determination with the NO• radical is 478 nm with maximum absorbance for an ethanol

extract of purwoceng leaves is 0,553, ethanol Capture 3. Electron Donation or Hydrogen Radical extract of purwoceng herbs is 0,553, quercetin is from Antioxidant to DPPH Radicals (Mun’im, A 0,446 and BHT is 0,443. This means that for 2008) power of free radicals scavenging test performed on maximum λ 478 nm.

ISBN 9786021493007 HM 443 Universitas Muhammadiyah Purwokerto Proceedings of International Conference On Education, Technology and Science (NETS) 2013 Purwokerto, December 28th, 2013

Table 2. Scavenging Power of Ethanol Extract of Leaves and Ethanol Extract of Purwoceng Herbs with DPPH Radicals %Inhibition %Inhibition %Inhibition Concentration Replication 1 Replication 2 Replication 3 (ppm) Herbs Leaves Herbs Leaves Herbs Leaves 50 33,59 25,13 38,49 26,46 39,42 27,66 40 28,17 20,61 33,33 22,21 34,39 22,74 30 21,29 15,43 24,47 16,62 25,79 17,82 20 16,01 11,44 19,05 13,03 20,50 13,83 10 5,82 6,38 8,33 6,78 9,39 7,58

Capture 4. The Reaction Between Nitric Ion with Sulphalinamide which Paired with naphtylethylendiamine (Wikipedia en, 2013)

Table 3. Scavenging Power of Ethanol Extract of Leaves and Ethanol Extract of Purwoceng Herbs with NO• Radicals %Inhibition %Inhibition %Inhibition Concentration Replication 1 Replication 2 Replication 3 (ppm) Herbs Leaves Herbs Leaves Herbs Leaves 50 43,22 54,07 38,52 48,64 40,51 40,87 40 33,45 50,99 32,91 44,30 35,98 39,06 30 32,73 49,91 29,11 43,76 28,75 38,52 20 28,75 41,41 25,49 34,36 21,52 27,31 10 24,95 29,11 20,79 23,87 18,81 18,99

Table 4. Quercetin Free Radical Scavenging Power %Inhibition %Inhibition %Inhibition Concentration Replication 1 Replication 2 Replication 3 (ppm) DPPH NO• DPPH NO• DPPH NO• 0,64 31,44 46,84 33,83 47,56 34,93 46,29 0,32 7,86 41,05 9,25 40,87 10,25 41,77 0,16 3,98 39,60 3,38 37,97 3,18 39,78 0,08 13,53 33,82 13,13 35,44 12,94 34,90 0,04 15,32 29,29 14,43 30,92 14,23 29,11

Table 5. BHT Free Radical Scavenging Power %Inhibition %Inhibition %Inhibition Concentration Replication 1 Replication 2 Replication 3 (ppm) DPPH NO• DPPH NO• DPPH NO• 8 55,56 74,32 67,99 69,08 69,71 65,28 4 32,14 70,88 42,33 66,00 43,65 60,22 2 18,52 63,47 25,66 58,23 26,72 53,89 1 4,49 47,56 7,28 41,41 7,94 43,39 0,5 2,78 41,77 5,29 35,55 5,56 36,71

Percent inhibition was analyzed to find out to inhibit 30% of free radicals. The higher of I% value know IC30 value, is using curve relationship accordingly the IC30 is lower. The lower of IC30 between percent barrier with a concentration make the better of free radical power inhibition. (Gulluce et al, 2006). The magnitude of free radical IC30 is used because if used IC50 the result can have scavenging power characterized by the IC30 value, extrapolation outside the range series of is the solution concentration of samples needed to concentration.

Universitas Muhammadiyah Purwokerto HM 444 ISBN 9786021493007 Proceedings of International Conference On Education, Technology and Science 2013 Purwokerto, December 28th, 2013

Table 6. IC30 Value of Ethanol Extract of Purwoceng Leaves, Ethanol Extract of Purwoceng Herbs and Positive Controls IC30 Value (ppm) DPPH NO• DPPH NO• DPPH NO• DPPH NO• Replication Ethanol Extract Ethanol Extract of Purwoceng of Purwoceng Quercetin BHT Leaves Herbs 1 43,33 4,62 43,33 23,67 43,33 0,48 43,33 0,48 2 37,06 14,89 37,06 31,47 37,06 0,47 37,06 0,47 3 38,57 24,69 38,57 31,51 38,57 0,48 38,57 0,48 IC30 average 38,65 14,73 38,65 28,88 38,65 0,48 38,65 0,48

From the value data of IC30 can be concluded Based on table 7. the IC30 value of ethanol that free radical scavenging power of ethanol extracts of purwoceng leaves and ethanol extract of extract of purwoceng leaves is better then ethanol purwoceng herbs by DPPH radical greater than by extract of purwoceng herbs. NO• racidal. This is possible because both radical have differences in how forms a stable compound. 4. Comparison of Free Radicals Scavenging Another possibility is a compound that plays a role Power in the free radicals scavenging from ethanol extract The use of different methods for determining of prwoceng leaves and ethanol extracts of the free radicals scavenging power of plant extracts purwoceng herbs namely flavonoids less reactive can shown the random results, it is difficult to against DPPH radical because of steric hindrance, compare and sometimes give rise to incompatibility so that only a small molecule that has a greater (Koleva et al. 2002 in Irawati 2008). opportunity to react with DPPH radical (Irawati, 2008).

Table 7. Comparison of IC30 Value Between DPPH Radical and NO• Radical IC Value (ppm) Ethanol Extract 30 DPPH Radical NO• Radical Purwoceng Leaves 38,65 14,73 Purwoceng Herbs 57,25 28,88

CONCLUSIONS AND SUGGESTIONS Backer, A.C & van der Brick, B.C.R. 1965. Flora It can be concluded that there is a difference of Java (Spermatophytes only), vol II. of free radicals scavenging power between ethanol Netherlands: N.V.P Noordhoff. Backer, A.C extract of purwoceng leaves and purwoceng herbs & van der Brick, B.C.R. 1965. Flora of Java by looking at the value of IC30. (Spermatophytes only), vol II. Netherlands: It is advisable to commit further research N.V.P Noordhoff. about the free radicals scavenging power for Caropeboka, A.M. dan I. Lubis, 1975, Pemeriksaan antother purwoceng leaves and herbs extract, both pendahuluan kandungan kimia akar petroleum ether extract or ethyl acetate extract. Pimpinella alpine (purwoceng), Dalam Types of flavonoids compounds in the leaves and Simposium Tanaman Obat I, 8 9 Desember, herbs of purwoceng who have power of free Bagian Farmakologi. FKH, Institut Pertanian radicals scavenging. As well as the use of other Bogor. methods to test the activity of antioxidants that can Caropeboka, A.M, Iskandar dan Paridjo, P, 1979, support free radical scavenging research of Pengaruh Ekstrak Akar Pimpinella alpine purwoceng plant so it can be known the right dose Koord. Terhadap Reproduksi Hewan, Dalam in order to declare that purwoceng plant is a natural Usmiati, S dan Yuliani, S, 2010, Efek antioxidants. Androgenik dan Anabolik Ekstrak Akar Pimpinella alpine Molk. (Purwoceng) pada Reference Anak Ayam Jantan, Balai Besar Penelitian dan Anonim, 1979, Farmakope Indonesia, Edisi III, Pengembangan Pascapanen Pertanian, Bogor. Jakarta, Departemen Kesehatan Republik Fessenden, Ralp J. dan Fessenden, Joan S., 1982, Indonesia. Kimia Organik Edisi Ketiga, Jakarta, Penerbit Erlangga.

ISBN 9786021493007 HM 445 Universitas Muhammadiyah Purwokerto Proceedings of International Conference On Education, Technology and Science (NETS) 2013 Purwokerto, December 28th, 2013

Gulluce M, Aslan A, Sokmen M, Sahin F, Adiguzel UNS Informasi Herbal, 2012, Purwoceng, A, Agar A & Sokmen A, 2006, Screening the http://www.informasiherbal.co m/purwoceng/, antioxidant and antimicrombial properties of [18 Desember 2012]. the lichens Parmelia saxatilis, Platismatis Irawati, Irma, 2008, Perbandingan Metode glauca, Ramalina pollinaria, Ramalina Penentuan Aktivitas Antioksidan Rimpang polymorpha and Umbilicaria nylamderiana, J. Temulawak, [skripsi], Bogor, Institut Phytomedicine 13 (2006) p.515 521. Pertaninan Bogor. Halliwell, B. & J.M.C. Gutteridge, 2000, Free Mun’im, A., 2008, Aktivitas Antioksidan Radical in Biology and Medicine. Ed 4th, New Cendawan Suku Pleurota dan Polyporaceae York, Oxford University Press. dari hutan UI, Jurnal Ilmiah Farmasi Haryono, Nugroho Tri, 2013, Purwoceng, Vol.5No.1, Jakarta. http://www.herbaltama.com/?T Waji, Resi Agestia dan Sugrani, Andi, 2009, anaman_Obat:Purwoceng [12 Mei 2013]. Flavonoid (Quercetin) [Makalah Kimia Hernawan, Udhi Eko Dan Setyawan, Ahmad Dwi, Organik Bahan Alam], Makassar, Program S2 2003, Ellagitanin; Biosintesis, Isolasi, dan Kimia, Fakultas Matematika dan Ilmu Aktivitas Biologi [Review], Surakarta, Jurusan Pengetahuan Alam, Universitas Hasanuddin. Biologi FMIPA. Wikipedia, 2013, Griess Test. http://en.m.wikipedia.org/wiki/ Griess_test. [24 Agustus 2013].

Universitas Muhammadiyah Purwokerto HM 446 ISBN 9786021493007