DOCSLIB.ORG

Histological Observation of Some of the Endocrine Glands in the Sterile Carp-Funa Hybrid (F1), with Special Reference to the Hypophysis*

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Histological Observation of Some of the Endocrine Glands in the Sterile Carp-Funa Hybrid (F1), with Special Reference to the Hypophysis* Arch. histol. jap., Vol. 42, No. 3 (1979) p. 305-318 Histological Observation of Some of the Endocrine Glands in the Sterile Carp-Funa Hybrid (F1), with Special Reference to the Hypophysis* Akira CHIBA,1 Yoshiharu HONMA,2 Sumio YOSHIE3 and Yoshio OJIMA4 Biological Laboratory1 and Department of Oral Anatomy,3 Nippon Dental University Niigata; Sado Marine Biological Station,2 Faculty of Science, Niigata University, Niigata and Department of Biology,4 Kansei Gakuin University, Nishinomiya, Japan Received November 9, 1978 Summary. Some endocrine glands of the carp-funa hybrid were studied with a light microscope to elucidate their detailed structure and the possible causal factor of sterility in the males. Adult specimens of carp (Cyprinus carpio), gengoroh-buna (Carassius auratus cuvieri), and their hybrid (F1) were examined. The hybrid males are sterile as manifested by the failure of meiosis and seminomat- ous neoplasm in their testes. The hybrid females revealed well-developed ovaries, but their fertility was not tested. The hybrid hypophysis shows an intermediate condition between the parent species in the grade of ramification of the pars nervosa into the pars intermedia. Among seven types of granular cells demonstrated in the adenohypophysis, certain degenerative and anomalous changes are recognized only in the gonadotrophs of the hybrid hypophysis, especially in the female. These changes are discussed as a possible cause of sterility. A considerable amount of aldehyde fuchsin stainable neurosecretory material occurs in the cells of the nucleus preopticus and in the pars nervosa. The nucleus lateralis tuberis exhibits a histologically healthy condition. Occasionally, the carp (Cyprinus carpio) and the funa (=crucian carp) (Carassius auratus) can mate and yield offspring under confinement. The previous studies including breeding experiment, macroscopical and microscopical anatomy, and cyto- logical research have shown that 1) a reciprocal crossing is possible to yield similar offspring, 2) the bigeneric hybrids exhibit a condition intermediate between the parent species in morphological and cytochemical characters, 3) the hybrid males are * This paper is dedicated to the memory of the late Prof. Dr. W. BARGMANN. One of the authors, Y. HONMA,as well as his wife, Mitoko KAWAMOTO-HONMA, was intimately ac- quainted with Prof. BARGMANN through the study of piscine neuroendocrinology and also through Prof. Y. SANG and Prof. T. FUJITA. Prof. BARGMANN liked to look at and to investigate fish very much. He enjoyed visiting aquaria whenever he came to Japan. On one of such occasions, Prof. BARGMANN was deeply interested in a peculiar bony fish, the remora, and Y. HONMA, at his request, presented several sucking disks to him for morphological examination. The result was reported by Prof. BARGMANN and his associates in Z. Zellforsch. 139 (1973), while the endocrine glands of the same fish were studied and reported by Y. HONMAin Arch. histol. jap. 37 (1974). 305 306 A. CHIBA et al.: usually sterile although a small number of hybrid females are found to be fertile, and sometimes, 4) the hybrids have gonads of an intersexual character (MAKING et al., 1955; MATSUI et al., 1956; OJIMA et al., 1961; KAFUKU,1976; OJIMA, 1978). Some interesting results have been reported by OZTAN(1960, 1963), who found that the sterile hybrids of the poeciliid fish could become fertile by administration of gonadotropins and that the nucleus lateralis tuberis of the hypothalamus consider- ed as the locus regulating the activity of the gonadotrophs was inactive in the sterile fish, judging from histophysiological criteria. However, information for endocrino- logical analysis on the sterility of the carp-funa hybrid is scanty, although a brief account on microscopical structure of the hybrid hypophysis was given by HONMA (1960). The present study thus intends to elucidate the detailed structure of some of the endocrine glands in the carp-funa hybrid, and especially to obtain information about the functional aspect of the hypothalamo-hypophyseal-gonadal system in this sterile fish. MATERIALS AND METHODS Six adult carp-funa hybrid (F1) raised in the Yamada Fish Cultural Pond attached to Hyogo Prefectural Fisheries Experimental Station were used in this study. The parent fish, six pond-cultured carp (Gyprinus carpio) obtained commercially from the suburb of Niigata City and six gengoroh-buna (Carassius auratus cuvieri) caught from the lower reaches of Shinano River in Niigata City were examined for a comparison of their endocrine structures. The size of the body, sex, and other records are sum- marized in Table 1. Immediately after decapitation, the brain with hypophysis, thyroid, interrenal gland, and gonads were removed and fixed in Bouin-Hollande-sublimate solution. Small pieces of the head kidney and gonads were immersed also in Bouin-Allen's mixture and Ciaccio's solution for detailed cytological study. These materials were dehydrated, embedded in paraffin, and cut serially 6-8μm thick in both sagittal and transverse directions. Sections were stained with the following stainings: aldehyde fuchsin (AF)-fast green-orange G, periodic acid Schiff (PAS)-fast green-orange G, azan trichrome, alcian blue (AB)-PAS-orange G, lead hematoxylin (PbH), Delafield's hematoxylin-eosin, Heidenhain's iron hematoxylin-fast green, AF-azan, AB-acid fuchsin, and PAS-PbH. RESULTS Ovary The three hybrid females examined have a pair of well-developed, voluminous ovar- ies. The gonosomatic index attaining 13.8±1.1 is comparable with that of the mature female carp (Table 1). The ovigerous folds are crowded with numerous vitello- genetic oocytes ranging from 300μm to 1mm in diameter (Fig. 1). There are im- mature yolkless oocytes, 50-180μm in diameter, and oogonial cysts intervening among the maturing oocytes. The micropyle apparatus is seldom elaborated in the Endocrine Glands of Sterile Carp-Funa Hybrid 307 Table 1. Data of the carp, gengoroh-buna, and their hybrid (F1) used in the study maturing follicles, although no histologic anomalies are detectable in them. Fur- thermore, the collapsed follicles are occasionally found surrounded by a dense mass of small cells of stromatous origin. The epithelial lining of the ovarian cavity is constructed from ciliated tall columnar cells. Testis Two of the three males have a pair of elongate testes with normal appearance, al- though an extraordinarily large, globular growth, diagnosed as seminomatous neo- plasm, was found in the visceral cavity of the remaining male. No spermatogenetic 1 2 Fig. 1. A part of the ovary of a carp-funa hybrid showing maturing oocytes laden with yolk globules. Delafield's hematoxylin-eosin. ×63 Fig. 2. A part of the sterile testis of a hybrid showing pycnosis (black arrow) and abnormal meiosis (white arrow) in the spermatocytes. Heidenhain's iron hematoxylin-fast green. ×250 308 A. CHIBA et al. cells were detected in this peculiar growth, which is exclusively replaced by large, poly- gonal cells having a large nucleus surrounded by rich, lightly stained cytoplasm. The histo- logic details of this neoplasm will be reported elsewhere. Tie testes of the former two males A are also sterile, because neither spermatozoa nor spermatids were discernible in the testi- cular lobules. As the result of failing meiosis, nuclear pycnosis and cell shrinkage were brought about in spermatocytes (Fig. 2). Suc- cessive absorption of the dead spermatogenetic B cells has resulted in the formation of a vacant cavity in the testicular lobules. Multiplica- tion of spermatogonia and their transforma- tion into spermatocytes appear to be unaffect- ed, however. No appreciable changes occur in Sertoli cells, interstitial cells, and the epi- thelial cells lining the seminal duct in the hybrids, in comparison with those in the fer- C tile carp and gengoroh-buna. Fig. 3. Diagram showing the construc- Hypophysis tion of the hypophysis in the The general structural patterns of the hypo- carp (A), gengoroh-buna (B) and their hybrid (C). The hypo- physis of the carp, gengoroh-buna, and their physis of the hybrid shows an hybrid are schematically shown in Figure 3. intermediate condition between A certain close resemblance is visible among those of the parent species in them. Externally, the gland is ovoid or ellip- the grade of ramification of the tic in shape and slightly depressed. The rostral PN toward the PI. Black (PN), vertical lines (RPD), solid circles pars distalis (RPD), the smallest portion of the (PPD), and white (PI). adenohypophysis, is situated dorso-caudally to the massive proximal pars distalis (PPD), and the pars intermedia (PI) located anterior to the PPD projects antero-ventrally. From the dorso-caudal portion of the gland, the pars nervosa (PN) invades deeply into the adenohypophysis, and extensively sends its complicated ramifications into the PI. The neurohypophyseal branches in the PI are very slender and highly intricate in the carp, but they are rather thick and simple in the gengoroh-buna. The hybrid, thereby, show an intermediate condition in this context. The PN is composed of neurosecretory axons, glial elements, and capillaries. A considerable amount of AF stainable neurosecretory material is concentrated at the distal portion of the PN. Furthermore, the AF negative fibers occasionally loaded with the acidophil Herring bodies are demonstrated in the stem region of the PN. The RPD is composed of three main types of cells: two predominating types of acidophil cells, tentatively named A1 and A2, and a type of basophil cell, called B1. They are compactly arranged and intermingled with one another (Fig. 4). The A1 cell corresponding to the adrenocorticotroph is a large cell of polygonal or fusiform shape, containing a rounded nucleus with a conspicuous nucleolus. The coarsely Endocrine Glands of Sterile Carp-Funa Hybrid 309 granular cytoplasm is stained intensely with PbH and tinged bluish green by AF- fast green-orange G or reddish pink by AF-azan triple stain. This type of cell occurs solitarily or in small groups throughout the RPD, even in the neural tissue.
Load more

Recommended publications
  • Vocabulario De Morfoloxía, Anatomía E Citoloxía Veterinaria
    Vocabulario de Morfoloxía, anatomía e citoloxía veterinaria (galego-español-inglés) Servizo de Normalización Lingüística Universidade de Santiago de Compostela COLECCIÓN VOCABULARIOS TEMÁTICOS N.º 4 SERVIZO DE NORMALIZACIÓN LINGÜÍSTICA Vocabulario de Morfoloxía, anatomía e citoloxía veterinaria (galego-español-inglés) 2008 UNIVERSIDADE DE SANTIAGO DE COMPOSTELA VOCABULARIO de morfoloxía, anatomía e citoloxía veterinaria : (galego-español- inglés) / coordinador Xusto A. Rodríguez Río, Servizo de Normalización Lingüística ; autores Matilde Lombardero Fernández ... [et al.]. – Santiago de Compostela : Universidade de Santiago de Compostela, Servizo de Publicacións e Intercambio Científico, 2008. – 369 p. ; 21 cm. – (Vocabularios temáticos ; 4). - D.L. C 2458-2008. – ISBN 978-84-9887-018-3 1.Medicina �������������������������������������������������������������������������veterinaria-Diccionarios�������������������������������������������������. 2.Galego (Lingua)-Glosarios, vocabularios, etc. políglotas. I.Lombardero Fernández, Matilde. II.Rodríguez Rio, Xusto A. coord. III. Universidade de Santiago de Compostela. Servizo de Normalización Lingüística, coord. IV.Universidade de Santiago de Compostela. Servizo de Publicacións e Intercambio Científico, ed. V.Serie. 591.4(038)=699=60=20 Coordinador Xusto A. Rodríguez Río (Área de Terminoloxía. Servizo de Normalización Lingüística. Universidade de Santiago de Compostela) Autoras/res Matilde Lombardero Fernández (doutora en Veterinaria e profesora do Departamento de Anatomía e Produción Animal.
    [Show full text]
  • Histology Histology
    HISTOLOGY HISTOLOGY ОДЕСЬКИЙ НАЦІОНАЛЬНИЙ МЕДИЧНИЙ УНІВЕРСИТЕТ THE ODESSA NATIONAL MEDICAL UNIVERSITY Áiáëiîòåêà ñòóäåíòà-ìåäèêà Medical Student’s Library Серія заснована в 1999 р. на честь 100-річчя Одеського державного медичного університету (1900–2000 рр.) The series is initiated in 1999 to mark the Centenary of the Odessa State Medical University (1900–2000) 1 L. V. Arnautova O. A. Ulyantseva HISTÎLÎGY A course of lectures A manual Odessa The Odessa National Medical University 2011 UDC 616-018: 378.16 BBC 28.8я73 Series “Medical Student’s Library” Initiated in 1999 Authors: L. V. Arnautova, O. A. Ulyantseva Reviewers: Professor V. I. Shepitko, MD, the head of the Department of Histology, Cytology and Embryology of the Ukrainian Medical Stomatologic Academy Professor O. Yu. Shapovalova, MD, the head of the Department of Histology, Cytology and Embryology of the Crimean State Medical University named after S. I. Georgiyevsky It is published according to the decision of the Central Coordinational Methodical Committee of the Odessa National Medical University Proceedings N1 from 22.09.2010 Навчальний посібник містить лекції з гістології, цитології та ембріології у відповідності до програми. Викладено матеріали теоретичного курсу по всіх темах загальної та спеціальної гістології та ембріології. Посібник призначений для підготовки студентів до практичних занять та ліцензійного екзамену “Крок-1”. Arnautova L. V. Histology. A course of lectures : a manual / L. V. Arnautova, O. A. Ulyantseva. — Оdessa : The Оdessa National Medical University, 2010. — 336 p. — (Series “Medical Student’s Library”). ISBN 978-966-443-034-7 The manual contains the lecture course on histology, cytology and embryol- ogy in correspondence with the program.
    [Show full text]
  • Observations on the Histology and Ultrastructure
    OBSERVATIONS ON THE HISTOLOGY AND ULTRASTRUCTURE OF THE PARS DISTALIS OF THE RABBIT HYPOPHYSIS IN ORGAN CULTURE A Thesis submitted for the degree of Doctor of Philosophy in the University of London by SNEHLATA PATHAK Department of Cellular Biology 1970 and Histology, St. Mary's Hospital Medical School, London W.2. ABSTRACT The histology and ultrastrueture of the pars distalis of the rabbit hypophysis was studied after different periods of organ culture, and the best technique for the maintenance of the maximum proportion of the explant was assessed by comparing cultures grown in different conditions. Explants in air with a medium buffered. with N.2-hydroxyethylpiperazine-N1-2- ethanesulphonic acid (HEPES), not previously used in organ culture, proved more satisfactory than explants in carbogen with bicarbonate buffered 199, and cultures were maintained for more than 3 weeks. Material from young animals survived better than from old. The survival of cells was assessed on the basis of their cytological integrity when explants were examined by light microscopy after specific staining and by electron microscopy; DNA and RNA fluorescence with acridine orange was a valuable indicator. Also, cell multiplication was identified by direct observation of mitosis, by the application of the colchicine technique and by autoradiography. During culture, prolactin cells showed physiological signs of secretion (demonstrated by combined culture with mammary gland) and morphological signs of an increase of secretory activity. Morphological signs of reduced secretory activity appeared in the presence of hypothalamic tissue (combined culture) or extract. Somatotrophs and gonadotrophs. showed signs of low-level secretory activity in solitary pars distalis culture and of increased activity in combined culture with hypothalamus.
    [Show full text]
  • Involvement of Gap Junctional Communication in Secretion
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Biochimica et Biophysica Acta 1719 (2005) 82 – 101 http://www.elsevier.com/locate/bba Review Involvement of gap junctional communication in secretion Laetitia Michon 1, Rachel Nlend Nlend 1, Sabine Bavamian, Lorraine Bischoff, Nathalie Boucard, Dorothe´e Caille, Jose´ Cancela, Anne Charollais, Eric Charpantier, Philippe Klee, Manon Peyrou, Ce´line Populaire, Laurence Zulianello, Paolo Meda * Department of Cell Physiology and Metabolism, University of Geneva, C.M.U., 1 rue Michel Servet, 1211 Geneva 4, Switzerland Received 11 July 2005; received in revised form 31 October 2005; accepted 7 November 2005 Available online 18 November 2005 Abstract Glands were the first type of tissues in which the permissive role of gap junctions in the cell-to-cell transfer of membrane-impermeant molecules was shown. During the 40 years that have followed this seminal finding, gap junctions have been documented in all types of multicellular secretory systems, whether of the exocrine, endocrine or pheromonal nature. Also, compelling evidence now indicates that gap junction-mediated coupling, and/or the connexin proteins per se, play significant regulatory roles in various aspects of gland functions, ranging from the biosynthesis, storage and release of a variety of secretory products, to the control of the growth and differentiation of secretory cells, and to the regulation of gland morphogenesis. This review summarizes this evidence in the light of recent reports. D 2005 Elsevier B.V. All rights reserved. Keywords: Exocrine gland; Endocrine gland; Enzyme; Hormone; Ca2+; Synchronization Contents 1.
    [Show full text]
  • Light and Electron Microscopic Observations on the Anterior Pituitary of the Mouse Injected with Dl-Thyroxine By
    Okajimas Fol. anat. jap., 43: 21-51, 1967 Light and Electron Microscopic Observations on the Anterior Pituitary of the Mouse injected with dl-Thyroxine By Tomiji Uchida Department of Anatomy, Nagoya University School of Medicine, Nagoya, Japan (Director : Prof. Dr. Ka z u m a r o Y a m ad a) Introduction Probably, the first suggestion of the existence of a functional relationship between the thyroid and hypophysis was given by Niepce (1851) who described pituitary enlargement in a series of goitrous cretins. Since then numerous studies have provided detailed evidence of this relationship. The reciprocal interrelationship be- tween thyroid stimulating hormone (TSH) secretion in the anterior hypophysis and the circulating levels of thyroid hormone has been well established, and in general, procedures which reduce effective plasma concentrations of thyroid hormone increase thyrotrophic hormone release in the anterior pituitary, whereas increased circulat- ing levels of thyroxine inhibit thyrotophic hormone secretion. This negative feedback mechanism resides both in the level of the pituitary and of a hypothalamic " TSH releasing center " (S o 1 o m on and Dowling, '60). Earlier views (Ma rin e, Rosen and Spar k, '35; Morris, '52) favored pituitary acidophile cell as the source of thyrotrophic hormone, but results of many recent studies implicate the basophile cell (Zeckwer, '38a and '38b; Griesbach and Purves, '45 Pur v es and Griesbac h, '46a, '46b, '51a, '51b, '51c, '57a and '57b; Goldberg and Chaikoff, '50; Salter, '50, Halmi, '50, '51, '52a , '52b, and 52c ; R ennel s, '53; Halm i and G u d e, '54 D'Angelo, '53 and '55; Knigge, '55; Elf tman, '58; Mura - s h i m a, '60 and others).
    [Show full text]
  • Characterization of Human Pituitary Adenomas in Cell Cultures by Light and Electron Microscopic Morphology and Immunolabeling
    FOLIA HISTOCHEMICA ET CYTOBIOLOGICA Vol. 43, No. 2, 2005 pp. 81-90 Characterization of human pituitary adenomas in cell cultures by light and electron microscopic morphology and immunolabeling Ilona Fazekas1, Balázs Hegedüs1, Ernö Bácsy2, Edit Kerekes1, Felicia Slowik1, Katalin Bálint1 and Emil Pásztor1 1National Institute of Neurosurgery, Budapest, and 2Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary Abstract: The morphology and hormone production of pituitary adenoma cell cultures were compared in order to highlight their characteristic in vitro features. Cell suspensions were prepared from 494 surgical specimens. The 319 viable monolayer cultures were analyzed in detail by light microscopy and immunocytochemistry within two weeks of cultivation. Some cultures were further characterized by scanning, transmission and immunogold electron microscopy. The viability and detailed in vitro morphology of adenoma cells were found to be characteristic for the various types of pituitary tumors. The sparsely granulated growth hormone, the corticotroph and the acidophil stem cell adenomas provided the highest ratio of viable cultures. Occasionally, prolonged maintenance of cells resulted in long-term cultures. Furthermore, a variety of particular distributions of different hormone-containing granules were found in several cases. Both light microscopic and ultrastructural analyses proved that the primary cultures of adenoma cells retain their physiological features during in vitro cultivations. Our in vitro findings correlated with the routine histopathological examination. These results prove that monolayer cultures of pituitary adenoma cells can contribute to the correct diagnosis and are valid model systems for various oncological and neuroendoc- rinological studies. Key words: Pituitary adenoma - Hormone - Immunolabeling - Electron microscopy - Cell culture Introduction drugs and hormone therapies aimed at the control of tumor growth [1, 2, 10].
    [Show full text]
  • Índice De Denominacións Españolas
    VOCABULARIO Índice de denominacións españolas 255 VOCABULARIO 256 VOCABULARIO agente tensioactivo pulmonar, 2441 A agranulocito, 32 abaxial, 3 agujero aórtico, 1317 abertura pupilar, 6 agujero de la vena cava, 1178 abierto de atrás, 4 agujero dental inferior, 1179 abierto de delante, 5 agujero magno, 1182 ablación, 1717 agujero mandibular, 1179 abomaso, 7 agujero mentoniano, 1180 acetábulo, 10 agujero obturado, 1181 ácido biliar, 11 agujero occipital, 1182 ácido desoxirribonucleico, 12 agujero oval, 1183 ácido desoxirribonucleico agujero sacro, 1184 nucleosómico, 28 agujero vertebral, 1185 ácido nucleico, 13 aire, 1560 ácido ribonucleico, 14 ala, 1 ácido ribonucleico mensajero, 167 ala de la nariz, 2 ácido ribonucleico ribosómico, 168 alantoamnios, 33 acino hepático, 15 alantoides, 34 acorne, 16 albardado, 35 acostarse, 850 albugínea, 2574 acromático, 17 aldosterona, 36 acromatina, 18 almohadilla, 38 acromion, 19 almohadilla carpiana, 39 acrosoma, 20 almohadilla córnea, 40 ACTH, 1335 almohadilla dental, 41 actina, 21 almohadilla dentaria, 41 actina F, 22 almohadilla digital, 42 actina G, 23 almohadilla metacarpiana, 43 actitud, 24 almohadilla metatarsiana, 44 acueducto cerebral, 25 almohadilla tarsiana, 45 acueducto de Silvio, 25 alocórtex, 46 acueducto mesencefálico, 25 alto de cola, 2260 adamantoblasto, 59 altura a la punta de la espalda, 56 adenohipófisis, 26 altura anterior de la espalda, 56 ADH, 1336 altura del esternón, 47 adipocito, 27 altura del pecho, 48 ADN, 12 altura del tórax, 48 ADN nucleosómico, 28 alunarado, 49 ADNn, 28
    [Show full text]
  • Induced Oxidative Stress and Adult Wistar Rat Pituitary Gland Histology
    JBRA Assisted Reproduction 2019;23(2):117-122 doi: 10.5935/1518-0557.20190024 Original article Effects of Aqueous Leaf Extract of Lawsonia inermis on Aluminum- induced Oxidative Stress and Adult Wistar Rat Pituitary Gland Histology Toluwase Solomon Olawuyi1, Kolade Busuyi Akinola1, Sunday Aderemi Adelakun1, Babatunde Samson Ogunlade1, Grace Temitope Akingbade1 1Department of Anatomy, School of Health and Health Technology, Federal University of Technology, Akure (FUTA), Nigeria ABSTRACT The anterior pituitary gland contains numerous baso- Objectives: The aim of this study was to investigate phil cells. The counts of acidophil cells (arranged in cords) the antioxidant effect of aqueousLawsonia inermis leaf ex- were lower than the basophil and chromophobe cell counts. tract on aluminum-induced oxidative stress and the histol- Acidophil cells may occur in two different forms based on ogy of the pituitary gland of adult Wistar rats. their size and shape. Type 1 cells are found near the sinu- Methods: Thirty-five adult male Wistar rats weighing soids and have irregular shapes, while Type 11 cells are between 100-196g and 15 mice of the same weight range round and have coarse chromatin granules. Basophil cells were included in the study. Lawsonia inermis extracts and are larger and occur in greater number than acidophil cells. They are categorized into two types of different shapes and aluminum chloride (AlCl3) were administered for a period of three weeks to five rats per group. The subjects in Group sizes. Basophil cells stain magenta-bluish (Young et al., 1 (control) were given pellets and distilled water. Group 2006). Chromophobe cells are round and are the largest 2 received 60mg/kg/d of aqueous extract of Lawsonia in- of the group.
    [Show full text]
  • Quantitative and Histomorphological Studies on Age-Correlated Changes in Canine and Porcine Hypophysis Lakshminarayana Das Iowa State University
    Iowa State University Capstones, Theses and Retrospective Theses and Dissertations Dissertations 1971 Quantitative and histomorphological studies on age-correlated changes in canine and porcine hypophysis Lakshminarayana Das Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/rtd Part of the Animal Structures Commons, and the Veterinary Anatomy Commons Recommended Citation Das, Lakshminarayana, "Quantitative and histomorphological studies on age-correlated changes in canine and porcine hypophysis" (1971). Retrospective Theses and Dissertations. 4873. https://lib.dr.iastate.edu/rtd/4873 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Retrospective Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. 71-26,847 DAS, Lakshminarayana, 1936- QUANTITATIVE AND HISTOMORPHOLOGICAL STUDIES ON AGE-CORRELATED CHANGES IN CANINE AND PORCINE HYPOPHYSIS (VOLUMES I AND II). Iowa State University, Ph.D., 1971 Anatomy• University Microfilms, A XEROX Company, Ann Arbor. Michigan Quantitative and histomorphological studies on age-correlated changes in canine and porcine hypophysis py Lakshminarayana Das Volume 1 of 2 A Dissertation Submitted to the Graduate Faculty in Partial Fulfillment of The Requirements for the Degree of DOCTOR OP PHILOSOPHY Major Subject:
    [Show full text]
  • 2019 123 Age-Dependent Mapping of Somatotropic Hormone in Rat
    SCVMJ, XXIV (2) 2019 123 Age-Dependent Mapping of Somatotropic Hormone in Rat Pituitary Gland; Histological, Histochemical and Immunohistochemical Studies Abdel-Hamid Kamel Osman ([email protected]) Hussein Eidaroos Hussein ([email protected]) Amal Arfaat Mokhtar Ahmed (amal_ahmed122yahoo.com) Fatma Khodary Mohamed Ali ([email protected]) Department of cytology and histology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt. Abstract The current study was performed to investigate age-dependent immunoexpression and localization of somatotropin in the pituitary gland. Twenty adult Wistar rats (fifteen females and five males) were housed; four animals per cage with observation of pregnancy. Samples from pituitary gland were collected from different ages of male offsprings. Tissue specimens were processed, and stained with Hematoxylin and Eosin (H&E), special stain and immunohistochemical technique. Sections were divided into four stages. H&E results showed that at stage I, acidophils were the most distinguished cells; but basophils were difficult to be seen then appeared at stage II. Special stain results showed increasing in somatotrophs number from stage I to stage III then decreased at the last stage. Immunoexpression of somatotropin in pars distalis showed that somatotrophs were numerous and uniformly distributed at the first three stages then they were restricted in clusters at the last stage. Key words: somatotropin- immunoexpression- pituitary gland- rat. Introduction hormones, a stimulatory growth Somatotrophic hormone has hormone releasing hormone functional and biological effects as (GHRH), and an inhibitory one of the chief anabolic hormones somatostatin hormone whose functional role is vital for (SST)(Tannenbaum et al., 2003). growth and metabolism throughout The immunostained somatotrophs prenatal and postnatal development showing a granular cytoplasmic (Campbell, 1997, Lecomte et al., pattern and distributed 2018,Zhang et al., 2018).
    [Show full text]
  • Endocrine Glands
    ENDOCRINE GLANDS The different activities of the body are regulated by 2 main systems:- 1. The nervous system. 2. The endocrine system. The nervous system: is concerned with regulation of metabolic activities and secretions of glands. It is a rapid control system. The endocrine system: is concerned with regulation of metabolic functions. It is a slow control system. What is meant by the endocrine system? It is a system of ductless glands, which differs from other types of glands such as the salivary glands in that their secretions which are called hormones enter the blood stream directly which carries them to different tissues to produce their effects. What is a hormone ? It is a chemical substance secreted into the blood stream by endocrine glands to act on distant organs called effector organs. Properties of hormones: They are secreted in very small amounts. So, their blood and tissue concentrations are very low. They do not act on the organs secreting them (endocrine glands) but act on distant organs (effector organs). Hormones may act on a specific effector organ e.g. thyrotrophic hormone (TSH) of the anterior pituitary acts specifically on the thyroid gland, or hormones may act on the body as a whole e. g. growth hormone (GH) of the anterior pituitary. Hormones act on the target organ by changing the rate of biochemical reactions in that organ. This effect persists for a longer period even after the hormone which produced it becomes inactivated. Difference between a hormone and an enzyme: Hormone Enzyme Some hormones are All enzymes are proteins. proteins, some are amino acids and some are steroids.
    [Show full text]
  • Vocabulario De Morfoloxía, Anatomía E Citoloxía Veterinaria
    Vocabulario de Morfoloxía, anatomía e citoloxía veterinaria (galego-español-inglés) Servizo de Normalización Lingüística Universidade de Santiago de Compostela COLECCIÓN VOCABULARIOS TEMÁTICOS N.º 4 SERVIZO DE NORMALIZACIÓN LINGÜÍSTICA Vocabulario de Morfoloxía, anatomía e citoloxía veterinaria (galego-español-inglés) 2008 UNIVERSIDADE DE SANTIAGO DE COMPOSTELA VOCABULARIO de morfoloxía, anatomía e citoloxía veterinaria : (galego-español- inglés) / coordinador Xusto A. Rodríguez Río, Servizo de Normalización Lingüística ; autores Matilde Lombardero Fernández ... [et al.]. – Santiago de Compostela : Universidade de Santiago de Compostela, Servizo de Publicacións e Intercambio Científico, 2008. – 369 p. ; 21 cm. – (Vocabularios temáticos ; 4). - D.L. C 2458-2008. – ISBN 978-84-9887-018-3 1.Medicina veterinaria-Diccionarios.. 2.Galego (Lingua)-Glosarios, vocabularios, etc. políglotas. I.Lombardero Fernández, Matilde. II.Rodríguez Rio, Xusto A. coord. III. Universidade de Santiago de Compostela. Servizo de Normalización Lingüística, coord. IV.Universidade de Santiago de Compostela. Servizo de Publicacións e Intercambio Científico, ed. V.Serie. 591.4(038)=699=60=20 © Universidade de Santiago de Compostela, 2008 Coordinador Xusto A. Rodríguez Río (Área de Terminoloxía. Servizo de Normalización Lingüística. Universidade de Santiago de Compostela) Autoras/res Matilde Lombardero Fernández (doutora en Veterinaria e profesora do Departamento de Anatomía e Produción Animal. Universidade de Santiago de Compostela). Marcos Pérez López (doutor en Veterinaria e profesor do Departamento de Sanidad Animal. Universidad de Extremadura). Margarita Rico Gómez (doutora en Veterinaria e profesora do Departamento de Anatomía e Produción Animal. Universidade de Santiago de Compostela). Concepción Diéguez Diéguez (técnica lingüista. Reverso Servizos Lingüísticos) Xoán Antón Lado Lago (técnico lingüista. Reverso Servizos Lingüísticos) Nel Vidal Barral (técnico de normalización lingüística.
    [Show full text]