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Rpp40 (G-4): Sc-515079 SAN TA C RUZ BI OTEC HNOL OG Y, INC . Rpp40 (G-4): sc-515079 BACKGROUND APPLICATIONS Ribonuclease P (RNase P) removes the 5' leader sequences from precursor Rpp40 (G-4) is recommended for detection of Rpp40 of human origin by tRNA molecules. RNase P consists of an RNA species (H1 RNA), the POP1 Western Blotting (starting dilution 1:100, dilution range 1:100-1:1000), protein, and at least seven proteins called RPPs. The RPPs have apparent immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell molecular masses of 14 kD (Rpp14), 20 kD (Rpp20), 25 kD (Rpp25), 29 kD lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50- (Rpp29), 30 kD (Rpp30), 38 kD (Rpp38) and 40 kD (Rpp40). Rpp40 (Ribonu- 1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30- clease P protein subunit p40), also known as RNASEP1, is a 363 amino acid 1:3000). protein that localizes to nucleolus. Existing as two alternatively spliced Suitable for use as control antibody for Rpp40 siRNA (h): sc-95630, Rpp40 isoforms, the Rpp40 gene is conserved in chimpanzee, canine, bovine, mouse, shRNA Plasmid (h): sc-95630-SH and Rpp40 shRNA (h) Lentiviral Particles: rat, chicken and zebrafish, and maps to human chromosome 6p25.1. Making sc-95630-V. up nearly 6% of the human genome, chromosome 6 contains around 1,200 genes within 170 million base pairs of sequence. Notably, the PARK2 gene, Rpp40 (G-4) X TransCruz antibody is recommended for Gel Supershift and which is associated with Parkinson’s disease, and the genes encoding the ChIP applications. major histocompatiblity complex proteins, which are key molecular compo - Molecular Weight of Rpp40 isoforms: 42/39 kDa. nents of the immune system and determine predisposition to rheumatic dis eases, are all located on chromosome 6. Positive Controls: IMR-32 nuclear extract: sc-2148, HeLa nuclear extract: sc- 2120 or HeLa whole cell lysate: sc-2200. REFERENCES RECOMMENDED SUPPORT REAGENTS 1. Jarrous, N., et al. 1998. Autoantigenic properties of some protein subunits of catalytically active complexes of human ribonuclease P. RNA 4: 407- 417. To ensure optimal results, the following support reagents are recommended: 1) Western Blotting: use m-IgG κ BP-HRP: sc-516102 or m-IgG κ BP-HRP (Cruz 2. Jarrous, N., et al. 1999. Localization in the nucleolus and coiled bodies Marker): sc-516102-CM (dilution range: 1:1000-1:10000), Cruz Marker™ of protein subunits of the ribonucleoprotein ribonuclease P. J. Cell Biol. Molecular Weight Standards: sc-2035, UltraCruz ® Blocking Reagent: 146: 559-572. sc-516214 and Western Blotting Luminol Reagent: sc-2048. 2) Immunopre- 3. Jarrous, N., et al. 1999. Rpp14 and Rpp29, two protein subunits of human cipitation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). ribonuclease P. RNA 5: 153-157. 3) Immunofluorescence: use m-IgG κ BP-FITC: sc-516140 or m-IgG κ BP-PE: sc-516141 (dilution range: 1:50-1:200) with UltraCruz ® Mounting Medium: 4. Jiang, T., et al. 2001. Protein-RNA interactions in the subunits of human sc-24941 or UltraCruz ® Hard-set Mounting Medium: sc-359850. nuclear RNase P. RNA 7: 937-941. 5. Online Mendelian Inheritance in Man, OMIM™. 2001. Johns Hopkins DATA University, Baltimore, MD. MIM Number: 606117. World Wide Web URL: http://www.ncbi.nlm.nih.gov/omim/ ABC D AB 55K– 55K– CHROMOSOMAL LOCATION 43K– < Rpp40 43K– < 34K– Rpp40 Genetic locus: RPP40 (human) mapping to 6p25.1. 34K– 23K– SOURCE 23K– Rpp40 (G-4) is a mouse monoclonal antibody raised against amino acids 1-300 mapping at the N-terminus of Rpp40 of human origin. Rpp40 (G-4): sc-515079. Western blot analysis of Rpp40 Rpp40 (G-4): sc-515079. Western blot analysis of Rpp40 expression in IMR-32 (A) and HeLa (B) nuclear extracts, expression in IMR-32 nuclear extract (A) and T98G HeLa whole cell lysate (C) and human esophagus tissue whole cell lysate (B). PRODUCT extract (D). Each vial contains 200 µg IgG 2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Also available as TransCruz reagent for Gel STORAGE Supershift and ChIP applications, sc-515079 X, 200 µg/0.1 ml. Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of Rpp40 (G-4) is available conjugated to agarose (sc-515079 AC), 500 µg/0.25 ml shipment. Non-hazardous. No MSDS required. agarose in 1 ml, for IP; to HRP (sc-515079 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; to either phycoerythrin (sc-515079 PE), fluorescein (sc-515079 FITC), RESEARCH USE Alexa Fluor ® 488 (sc-515079 AF488), Alexa Fluor ® 546 (sc-515079 AF546), For research use only, not for use in diagnostic procedures. Alexa Fluor ® 594 (sc-515079 AF594) or Alexa Fluor ® 647 (sc-515079 AF647), Alexa Fluor ® is a trademark of Molecular Probes, Inc., Oregon, USA 200 µg/ml, for WB (RGB), IF, IHC(P) and FCM; and to either Alexa Fluor ® 680 (sc-515079 AF680) or Alexa Fluor ® 790 (sc-515079 AF790), 200 µg/ml, for Near-Infrared (NIR) WB, IF and FCM. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.
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