DOCSLIB.ORG

Investigation of the Genomics of Gender Regulation in Populus

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Investigation of the Genomics of Gender Regulation in Populus INVESTIGATION OF THE GENOMICS OF GENDER REGULATION IN POPULUS TRICHOCARPA by Nyssa A. Temmel B.Sc. (Hons) University of Victoria, 2002 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE FACULTY OF GRADUATE STUDIES (Botany) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) December 2011 © Nyssa A. Temmel, 2011 Abstract This thesis reports the findings of four projects conducted to study the genomics of gender regulation in Populus trichocarpa. Sex-linked markers previously discovered in Salix vimilanis were tested to determine if they were also sex-linked in other Salix species and P. trichocarpa. It was found that the DNA sequence of the SCAR 354 marker, and its position at the 5’ end of a gene encoding an Ssu72-like protein, was conserved with some SFP variability in species of Salix and P. trichocarpa. While this marker may be useful for phylogenetic or population studies in Salix, this marker was not sex-linked in the species investigated in this study. An investigation of genes located on the telomeric end of chromosome 19, the putative sex chromosome in P. trichocarpa, was conducted to look for gender-biased SNPs that would indicate recombination suppression in the region on a sex locus. A large variability in the number of SNPs was observed in the gene sequences studied, but no SNPs that segregated with gender were discovered so a genetic marker that could be used to sex P. trichocarpa individuals of unknown gender could not be developed. Using a microarray approach, gender-biased gene-expression was studied in leaf tissue of P. trichocarpa. While some gender-biased gene-expression was observed in vegetative tissues the differences observed were statistically insignificant due to biological variation in the samples tested, the small sample size used in this study, and changes in the genome annotation between version 1.1 and 2.0 of the poplar genome. This study could not verify the microarray results using rtPCR in a larger sample of male and female leaf tissue. MADS-box genes involved in floral development were identified as having gender- biased gene-expression using a microarray approach. Thirteen putative MADS-box genes ii that showed gender-biased expression in male and female inflorescences were discovered. Novel expression patterns for nine floral MADS-box genes were identified with this microarray data, and the expression patterns of three of these genes were investigated in further detail using reverse-transcription PCR. iii Preface A version of chapter two has been published. Temmel, Nyssa A., Rai, Hardeep S., and Cronk, Quentin C.B. (2007) Sequence characterization of the putative sex-linked Ssu72- like locus in willow and its homologue in poplar. Canadian Journal of Botany 85(11):1092- 1097 I wrote the majority of the manuscript and was responsible for all the laboratory work, while Dr. Rai contributed the DNA sequence editing and alignment, and Dr. Cronk assisted with the writing and the research was conducted in his laboratory. In chapter three and four, Dr. Rick White, the Managing Director of the Statistical Consulting and Research Laboratory at the University of British Columbia, did the statistical analysis of the microarray data. Collection of the P. trichocarpa leaf and floral buds from trees on the UBC Vancouver campus that were used for RNA, DNA and cDNA preparation was assisted by Collin Varner, the Horticulturist/Arborculturist with UBC Plant Operations and Gregg Doughty, the UBC Plant Operations arborist. iv Table of Contents Abstract .............................................................................................................................. ii Preface ................................................................................................................................iv Table of Contents ................................................................................................................v List of Tables................................................................................................................... xiii List of Figures...................................................................................................................xiv List of Abbreviations........................................................................................................xvi Acknowledgements..........................................................................................................xvii Dedication.........................................................................................................................xix Chapter 1: Introduction.....................................................................................................1 1.1 Sexual systems in plants ...........................................................................................1 1.2 Evolution of dioecy ..................................................................................................3 1.2.1 Gynodioecious pathway .....................................................................................3 1.2.2 Heterostyly pathway ..........................................................................................4 1.2.3 Monoecious pathway .........................................................................................5 1.2.4 Evolution of dioecy in Populus ..........................................................................5 1.3 Sex chromosomes in plants.......................................................................................6 1.3.1 Identification and description of sex chromosomes ............................................7 1.3.2 A brief history of the discovery of sex chromosomes .........................................7 1.3.3 Discovery of sex chromosomes in plants............................................................8 1.3.4 Evolution of sex chromosomes...........................................................................9 1.3.5 Proposed mechanism for the evolution of sex chromosomes ............................10 v 1.3.6 Degeneration of the Y chromosome .................................................................12 1.3.7 The absence of sex chromosomes in Populus ...................................................14 1.4 The genetics of gender............................................................................................14 1.4.1 Gender-determining genes ...............................................................................15 1.4.2 Gender-differentiation and development in plants ............................................16 1.4.3 Gender-differentiation in Zea mays ..................................................................17 1.4.4 Gender-differentiation in Cucumis sativus and C. melo ....................................18 1.4.5 The role of MADS box genes in gender differentiation ....................................21 1.4.6 Gender-associated genes ..................................................................................23 1.4.7 Differences in genetic control of gender-determination between monoecy and dioecy …………………………………………………………………………………. 24 1.5 Populus trichocarpa as a model organism for studying gender-determination in plants...............................................................................................................................25 1.5.1 Life history of Populus trichocarpa .................................................................25 1.5.2 Characteristics of Populus trichocarpa as a model species ...............................26 1.5.3 Genomic markers linked to sex-differentiation in Populus and Salix ................27 1.5.4 Gender-determination and chromosome 19 in Populus.....................................28 1.6 Research objectives ................................................................................................30 Chapter 2: Characterization of sex-linked genetic markers developed in Salix viminalis in other Salix species and Populus trichocarpa.................................................................31 2.1 Introduction............................................................................................................31 2.1.1 Sex-linked genetic markers in Salix..................................................................32 2.2 Objectives...............................................................................................................33 vi 2.3 Methods..................................................................................................................34 2.3.1 Collection and preparation of plant materials ...................................................34 2.3.2 PCR conditions and sequencing of samples......................................................35 2.3.3 Sequencing alignment for the SCAR sequences amplified in Salix...................36 2.3.4 Novel primer design based on SCAR sequence and associated coding gene regions in P. trichocarpa .............................................................................................36 2.3.5 Cloning, sequencing and phylogenetic analysis of S. arctica and S. reticulata samples .......................................................................................................................37 2.4 Results....................................................................................................................38 2.4.1 Amplification and characterization of the SCAR 354 marker ...........................38 2.4.2 Gene-anchored amplification
Load more

Recommended publications
  • Willows of Interior Alaska
    1 Willows of Interior Alaska Dominique M. Collet US Fish and Wildlife Service 2004 2 Willows of Interior Alaska Acknowledgements The development of this willow guide has been made possible thanks to funding from the U.S. Fish and Wildlife Service- Yukon Flats National Wildlife Refuge - order 70181-12-M692. Funding for printing was made available through a collaborative partnership of Natural Resources, U.S. Army Alaska, Department of Defense; Pacific North- west Research Station, U.S. Forest Service, Department of Agriculture; National Park Service, and Fairbanks Fish and Wildlife Field Office, U.S. Fish and Wildlife Service, Department of the Interior; and Bonanza Creek Long Term Ecological Research Program, University of Alaska Fairbanks. The data for the distribution maps were provided by George Argus, Al Batten, Garry Davies, Rob deVelice, and Carolyn Parker. Carol Griswold, George Argus, Les Viereck and Delia Person provided much improvement to the manuscript by their careful editing and suggestions. I want to thank Delia Person, of the Yukon Flats National Wildlife Refuge, for initiating and following through with the development and printing of this guide. Most of all, I am especially grateful to Pamela Houston whose support made the writing of this guide possible. Any errors or omissions are solely the responsibility of the author. Disclaimer This publication is designed to provide accurate information on willows from interior Alaska. If expert knowledge is required, services of an experienced botanist should be sought. Contents
    [Show full text]
  • Poplars and Willows: Trees for Society and the Environment / Edited by J.G
    Poplars and Willows Trees for Society and the Environment This volume is respectfully dedicated to the memory of Victor Steenackers. Vic, as he was known to his friends, was born in Weelde, Belgium, in 1928. His life was devoted to his family – his wife, Joanna, his 9 children and his 23 grandchildren. His career was devoted to the study and improve- ment of poplars, particularly through poplar breeding. As Director of the Poplar Research Institute at Geraardsbergen, Belgium, he pursued a lifelong scientific interest in poplars and encouraged others to share his passion. As a member of the Executive Committee of the International Poplar Commission for many years, and as its Chair from 1988 to 2000, he was a much-loved mentor and powerful advocate, spreading scientific knowledge of poplars and willows worldwide throughout the many member countries of the IPC. This book is in many ways part of the legacy of Vic Steenackers, many of its contributing authors having learned from his guidance and dedication. Vic Steenackers passed away at Aalst, Belgium, in August 2010, but his work is carried on by others, including mem- bers of his family. Poplars and Willows Trees for Society and the Environment Edited by J.G. Isebrands Environmental Forestry Consultants LLC, New London, Wisconsin, USA and J. Richardson Poplar Council of Canada, Ottawa, Ontario, Canada Published by The Food and Agriculture Organization of the United Nations and CABI CABI is a trading name of CAB International CABI CABI Nosworthy Way 38 Chauncey Street Wallingford Suite 1002 Oxfordshire OX10 8DE Boston, MA 02111 UK USA Tel: +44 (0)1491 832111 Tel: +1 800 552 3083 (toll free) Fax: +44 (0)1491 833508 Tel: +1 (0)617 395 4051 E-mail: [email protected] E-mail: [email protected] Website: www.cabi.org © FAO, 2014 FAO encourages the use, reproduction and dissemination of material in this information product.
    [Show full text]
  • Climatic Change Can Influence Species Diversity Patterns and Potential Habitats of Salicaceae Plants in China
    Article Climatic Change Can Influence Species Diversity Patterns and Potential Habitats of Salicaceae Plants in China WenQing Li 1, MingMing Shi 1, Yuan Huang 2, KaiYun Chen 1, Hang Sun 1 and JiaHui Chen 1,* 1 CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China; [email protected] (W.L.); [email protected] (M.S.); [email protected] (K.C.); [email protected] (H.S.) 2 School of Life Sciences, Yunnan Normal University, Kunming, Yunnan 650092, China; [email protected] * Correspondence: [email protected] (J.C.) Received: 18 January 2019; Accepted: 25 February 2019; Published: 1 March 2019 Abstract: Salicaceae is a family of temperate woody plants in the Northern Hemisphere that are highly valued, both ecologically and economically. China contains the highest species diversity of these plants. Despite their widespread human use, how the species diversity patterns of Salicaceae plants formed remains mostly unknown, and these may be significantly affected by global climate warming. Using past, present, and future environmental data and 2673 georeferenced specimen records, we first simulated the dynamic changes in suitable habitats and population structures of Salicaceae. Based on this, we next identified those areas at high risk of habitat loss and population declines under different climate change scenarios/years. We also mapped the patterns of species diversity by constructing niche models for 215 Salicaceae species, and assessed the driving factors affecting their current diversity patterns. The niche models showed Salicaceae family underwent extensive population expansion during the Last Inter Glacial period but retreated to lower latitudes during and since the period of the Last Glacial Maximum.
    [Show full text]
  • Nova Scotia Provincial Status Report on Hoary Willow Salix Candida
    i Nova Scotia Provincial Status Report on Hoary Willow Salix candida Flűeggé ex Willd. prepared for The Nova Scotia Species at Risk Working Group by Ruth E. Newell E.C. Smith Herbarium K.C. Irving Environmental Science Centre Acadia University Wolfville, Nova Scotia B4P 2R6 Funding provided by the Nova Scotia Species at Risk Conservation Fund Submitted December 16th, 2010 ii EXECUTIVE SUMMARY Wildlife Species Description and Significance Salix candida (Hoary Willow) is a low, deciduous, dioecious shrub, densely white woolly on current season’s twigs and lower leaf surfaces. The mature medial leaves are narrowly elliptic or oblanceolate, usually at least 4x as long as wide. Leaf margins are entire and slightly to strongly rolled under. Flowering occurs concurrently with leaf emergence. Female flowers have stalks 0.1 to 1.2 mm long and tomentose pistils. The anthers of male flowers are purple later changing to yellow. The fruit is a tomentose, pear-shaped capsule. Reproduction is both sexual and asexual by layering. Salix candida is an extremely rare species in Nova Scotia occurring in a rare habitat type i.e., rich, calcareous fens or marshes. Distribution In Nova Scotia, Hoary Willow occurs within the Black River system at the northwest end of Lake Ainslie, Inverness County, Cape Breton Island. Here it is known from four rich calcareous fens in close proximity to the river floodplain plus a single plant in a calcareous graminoid marsh. Field work failed to confirm the presence of Salix candida in Huntington, Cape Breton County - a record based on a herbarium specimen from Cape Breton University herbarium.
    [Show full text]
  • Poplar Chap 1.Indd
    Populus: A Premier Pioneer System for Plant Genomics 1 1 Populus: A Premier Pioneer System for Plant Genomics Stephen P. DiFazio,1,a,* Gancho T. Slavov 1,b and Chandrashekhar P. Joshi 2 ABSTRACT The genus Populus has emerged as one of the premier systems for studying multiple aspects of tree biology, combining diverse ecological characteristics, a suite of hybridization complexes in natural systems, an extensive toolbox of genetic and genomic tools, and biological characteristics that facilitate experimental manipulation. Here we review some of the salient biological characteristics that have made this genus such a popular object of study. We begin with the taxonomic status of Populus, which is now a subject of ongoing debate, though it is becoming increasingly clear that molecular phylogenies are accumulating. We also cover some of the life history traits that characterize the genus, including the pioneer habit, long-distance pollen and seed dispersal, and extensive vegetative propagation. In keeping with the focus of this book, we highlight the genetic diversity of the genus, including patterns of differentiation among populations, inbreeding, nucleotide diversity, and linkage disequilibrium for species from the major commercially- important sections of the genus. We conclude with an overview of the extent and rapid spread of global Populus culture, which is a testimony to the growing economic importance of this fascinating genus. Keywords: Populus, SNP, population structure, linkage disequilibrium, taxonomy, hybridization 1Department of Biology, West Virginia University, Morgantown, West Virginia 26506-6057, USA; ae-mail: [email protected] be-mail: [email protected] 2 School of Forest Resources and Environmental Science, Michigan Technological University, 1400 Townsend Drive, Houghton, MI 49931, USA; e-mail: [email protected] *Corresponding author 2 Genetics, Genomics and Breeding of Poplar 1.1 Introduction The genus Populus is full of contrasts and surprises, which combine to make it one of the most interesting and widely-studied model organisms.
    [Show full text]
  • A Guide to the Identification of Salix (Willows) in Alberta
    A Guide to the identification of Salix (willows) in Alberta George W. Argus 2008 Devonian Botanical Garden Workshop on willow identification Jasper National Park, Alberta 2 Available from: George W. Argus 310 Haskins Rd, Merrickville R3, Ontario, Canada K0G 1N0 email: [email protected] http://aknhp.uaa.alaska.edu/willow/index.html 3 CONTENTS Preface............................................................................................................................... 5 Salicaceae ...........…………………...........……........................................……..........…. 8 Classification ..........……………….…..….................................................….............…. 9 Some Useful Morphological Characters .......................................................….............. 11 Key to the Species.............................................................................................................13 Taxonomic Treatment .........................................................…..……….………............ 18 Glossary .....………………………………………....…..................………...........….... 61 Cited and Selected References ......................................................................................... 64 Salix Web Sites ...................……..................................……..................……............…. 68 Distribution Maps ............................................................................................................ 69 TABLES Table 1. Comparison of Salix athabascensis and Salix pedicellaris ..............................
    [Show full text]
  • Biodiversity Baseline in the Different Stages of the Project for the 10 Most Important Species
    Drafted by: November 2018 Baseline report of amphibians, reptiles, and Haptanthus hazlettii Jilamito Hydroelectric Project Baseline study of amphibians, reptiles and the arboreal species Haptanthus hazlettii in the site of the Jilamito Hydroelectric Project FINAL REPORT Research team: Ricardo Matamoros (Main Coordinator) José Mario Solís Ramos (Herpetologist – Field Coordinator) Carlos M. O'Reilly (Botanist) Josué Ramos Galdámez (Herpetologist) Juan José Rodríguez (Field Technician) Dilma Daniela Rivera (Field Technician) Rony E. Valle (Field Technician) Technical support team and local guides: Hegel Velásquez (INGELSA Technician) - Forest Engineer Omar Escalante (INGELSA Environmental Technician) Nelson Serrano (ICF Tela Technician) Mauro Zavala (PROLANSATE Technician) Alberto Ramírez (Field Guide) José Efraín Sorto (Field Guide) Juan Ramírez (Field Guide) Agustín Sorto Natarén (Field Guide) Manuel Sorto Natarén (Field Guide) José Hernán Flores (Field Guide) Photos on the cover: The arboreal species, Haptanthus hazlettii, found in bloom. In the pictures we observe: Plectrohyla chrysopleura (Climbing frog), Atlantihyla spinipollex (Ceiba stream frog), Duellmanohyla salvavida (Honduran brook frog), Pleistioson sumichrastri (blue tail lizard), Bothriechis guifarroi (green Tamagas, palm viper). 2 Baseline report of amphibians, reptiles, and Haptanthus hazlettii Jilamito Hydroelectric Project 1 Content 2. SUMMARY ...................................................................................................................... 5 3. INTRODUCTION
    [Show full text]
  • Number 3, Spring 1998 Director’S Letter
    Planning and planting for a better world Friends of the JC Raulston Arboretum Newsletter Number 3, Spring 1998 Director’s Letter Spring greetings from the JC Raulston Arboretum! This garden- ing season is in full swing, and the Arboretum is the place to be. Emergence is the word! Flowers and foliage are emerging every- where. We had a magnificent late winter and early spring. The Cornus mas ‘Spring Glow’ located in the paradise garden was exquisite this year. The bright yellow flowers are bright and persistent, and the Students from a Wake Tech Community College Photography Class find exfoliating bark and attractive habit plenty to photograph on a February day in the Arboretum. make it a winner. It’s no wonder that JC was so excited about this done soon. Make sure you check of themselves than is expected to seedling selection from the field out many of the special gardens in keep things moving forward. I, for nursery. We are looking to propa- the Arboretum. Our volunteer one, am thankful for each and every gate numerous plants this spring in curators are busy planting and one of them. hopes of getting it into the trade. preparing those gardens for The magnolias were looking another season. Many thanks to all Lastly, when you visit the garden I fantastic until we had three days in our volunteers who work so very would challenge you to find the a row of temperatures in the low hard in the garden. It shows! Euscaphis japonicus. We had a twenties. There was plenty of Another reminder — from April to beautiful seven-foot specimen tree damage to open flowers, but the October, on Sunday’s at 2:00 p.m.
    [Show full text]
  • Salix L.) in the European Alps
    diversity Review The Evolutionary History, Diversity, and Ecology of Willows (Salix L.) in the European Alps Natascha D. Wagner 1 , Li He 2 and Elvira Hörandl 1,* 1 Department of Systematics, Biodiversity and Evolution of Plants (with Herbarium), University of Goettingen, Untere Karspüle 2, 37073 Göttingen, Germany; [email protected] 2 College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China; [email protected] * Correspondence: [email protected] Abstract: The genus Salix (willows), with 33 species, represents the most diverse genus of woody plants in the European Alps. Many species dominate subalpine and alpine types of vegetation. Despite a long history of research on willows, the evolutionary and ecological factors for this species richness are poorly known. Here we will review recent progress in research on phylogenetic relation- ships, evolution, ecology, and speciation in alpine willows. Phylogenomic reconstructions suggest multiple colonization of the Alps, probably from the late Miocene onward, and reject hypotheses of a single radiation. Relatives occur in the Arctic and in temperate Eurasia. Most species are widespread in the European mountain systems or in the European lowlands. Within the Alps, species differ eco- logically according to different elevational zones and habitat preferences. Homoploid hybridization is a frequent process in willows and happens mostly after climatic fluctuations and secondary contact. Breakdown of the ecological crossing barriers of species is followed by introgressive hybridization. Polyploidy is an important speciation mechanism, as 40% of species are polyploid, including the four endemic species of the Alps. Phylogenomic data suggest an allopolyploid origin for all taxa analyzed Citation: Wagner, N.D.; He, L.; so far.
    [Show full text]
  • Agroforestry News Index Vol 1 to Vol 22 No 2
    Agroforestry News Index Vol 1 to Vol 22 No 2 2 A.R.T. nursery ..... Vol 2, No 4, page 2 Acorns, edible from oaks ..... Vol 5, No 4, page 3 Aaron, J R & Richards: British woodland produce (book review) ..... Acorns, harvesting ..... Vol 5, No 4, Vol 1, No 4, page 34 page 3 Abies balsamea ..... Vol 8, No 2, page Acorns, nutritional composition ..... 31 Vol 5, No 4, page 4 Abies sibirica ..... Vol 8, No 2, page 31 Acorns, removing tannins from ..... Vol 5, No 4, page 4 Abies species ..... Vol 19, No 1, page 13 Acorns, shelling ..... Vol 5, No 4, page 3 Acca sellowiana ..... Vol 9, No 3, page 4 Acorns, utilisation ..... Vol 5, No 4, page 4 Acer macrophyllum ..... Vol 16, No 2, page 6 Acorus calamus ..... Vol 8, No 4, page 6 Acer pseudoplatanus ..... Vol 3, No 1, page 3 Actinidia arguta ..... Vol 1, No 4, page 10 Acer saccharum ..... Vol 16, No 1, page 3 Actinidia arguta, cultivars ..... Vol 1, No 4, page 14 Acer saccharum - strawberry agroforestry system ..... Vol 8, No 1, Actinidia arguta, description ..... Vol page 2 1, No 4, page 10 Acer species, with edible saps ..... Vol Actinidia arguta, drawings ..... Vol 1, 2, No 3, page 26 No 4, page 15 Achillea millefolium ..... Vol 8, No 4, Actinidia arguta, feeding & irrigaton page 5 ..... Vol 1, No 4, page 11 3 Actinidia arguta, fruiting ..... Vol 1, Actinidia spp ..... Vol 5, No 1, page 18 No 4, page 13 Actinorhizal plants ..... Vol 3, No 3, Actinidia arguta, nurseries page 30 supplying ..... Vol 1, No 4, page 16 Acworth, J M: The potential for farm Actinidia arguta, pests and diseases forestry, agroforestry and novel tree ....
    [Show full text]
  • CYCADACEAE Palm-Like Dioecious Plants, the Caudex Bulb
    CYCADACEAE Palm-like dioecious plants, the caudex bulb-like, tuber­ like or columnar, simple or branched, few or several leaves borne from the apex; leaves spirally arranged, often a dense crown on the apex of the stem, coriaceous, pinnate or bipinnate, the leaflets usually linear or lanceolate, entire or dentate; inflo­ rescence borne at the apex of the caudex or lateral, cone-like, the pistillate eones broader than the staminate ones. Three genera native in Central America and about nine in the tropics of the world. Ceratozamia mexicana Brongn. is an attractive plant of Mexico and Guatemala that should be in cultivation. Dioon mejiae Standl. & L. Wms. Ceiba 1: 37. 1950. Teosinte, palma teosinte. The Indians of Olancho, Honduras, where the plant is native, boil and grind the large chestnut-like seeds and make of them a kind of tortilla of good flavor and agreeable to eat. The leaves are in demand for decorating altars and for funeral wreaths. Becoming widely distributed as an ornamental. The common name given for this plant is usually that applied to Euchlaena mexicana. Zamia loddigesii Miq. Tijdschr. Nat. Geschied. 10: 73. 1843. Camotillo, cocalito, teosinte, chacuhua. The large starchy roots are said to be used in poisoning rodents in Petén. There are reports of their use in criminal poisonings which may be or not based on fact. Standley reports that "It is stated, further, that if the root has been out of the ground three days, they cause death in three days; if dug ten days, they kill in ten days, and so on." The coastal Caribs have used the roots as food, after cooking.
    [Show full text]
  • Guide to the Willows of Shoshone National Forest
    United States Department of Agriculture Guide to the Willows Forest Service Rocky Mountain Research Station of Shoshone National General Technical Report RMRS-GTR-83 Forest October 2001 Walter Fertig Stuart Markow Natural Resources Conservation Service Cody Conservation District Abstract Fertig, Walter; Markow, Stuart. 2001. Guide to the willows of Shoshone National Forest. Gen. Tech. Rep. RMRS-GTR-83. Ogden, UT: U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station. 79 p. Correct identification of willow species is an important part of land management. This guide describes the 29 willows that are known to occur on the Shoshone National Forest, Wyoming. Keys to pistillate catkins and leaf morphology are included with illustrations and plant descriptions. Key words: Salix, willows, Shoshone National Forest, identification The Authors Walter Fertig has been Heritage Botanist with the University of Wyoming’s Natural Diversity Database (WYNDD) since 1992. He has conducted rare plant surveys and natural areas inventories throughout Wyoming, with an emphasis on the desert basins of southwest Wyoming and the montane and alpine regions of the Wind River and Absaroka ranges. Fertig is the author of the Wyoming Rare Plant Field Guide, and has written over 100 technical reports on rare plants of the State. Stuart Markow received his Masters Degree in botany from the University of Wyoming in 1993 for his floristic survey of the Targhee National Forest in Idaho and Wyoming. He is currently a Botanical Consultant with a research emphasis on the montane flora of the Greater Yellowstone area and the taxonomy of grasses. Acknowledgments Sincere thanks are extended to Kent Houston and Dave Henry of the Shoshone National Forest for providing Forest Service funding for this project.
    [Show full text]