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Supplementary Tables.Xlsx Supplementary table S4 – Comparison of the ScHaa1- and CgHaa1- dependent regulatory networks. The dataset of C. glabrata genes herein found to be regulated by CgHaa1 under acetic acid stress was compared with the dataset of genes described to be regulated by ScHaa1, based on the information available at the YEASTRACT database. Identification of orthologues was performed using the Yeastmine algorithm available at the Candida Genome database website after which a manual curation of the data based on BLASTP was performed. The promoter region of S. cerevisiae or C. glabrata genes was searched for the described ScHaa1 binding site (5’-(G/C)(A/C)GG(G/C)-3’, designated Haa1-responsive element – HRE; Mira et al., 2011) using the DNA pattern matching algorithms embedded in the RSA tools website. Cells highlighted in grey correspond to the S. cerevisiae genes found to be regulated by ScHaa1 specifically under acetic acid stress, based on the dataset of Mira et al., (2010). Acetic acid-responsive genes regulated by ScHaa1 only C. glabrata S. cerevisiae S. cerevisiae ORF Function promoter harbors promoter harbors orthologue an HRE motif an HRE motif Ortholog(s) have actin binding, cytoskeletal regulatory protein binding, enzyme activator CAGL0A00891g YLR319C No No activity Ortholog(s) have role in double-strand break repair, meiotic sister chromatid segregation, CAGL0A00913g recombinational repair, replication fork processing and Cul8-RING ubiquitin ligase complex, YLR320W No No nucleus localization CAGL0A01001g No Description Available YLR326W Yes No Ortholog(s) have nicotinamide-nucleotide adenylyltransferase activity, role in NAD biosynthetic CAGL0A01023g YLR328W Yes Yes process and cytosol, nucleus localization Ortholog(s) have proton-transporting ATP synthase activity, rotational mechanism activity, role CAGL0A01111g in ATP synthesis coupled proton transport and mitochondrial proton-transporting ATP synthase, YPL271W Yes Yes central stalk localization Ortholog(s) have pre-mRNA 5'-splice site binding, structural constituent of ribosome activity, CAGL0A01540g role in negative regulation of mRNA splicing, via spliceosome, rRNA processing and cytosol YGL030W No No localization 598 CAGL0A01760g Ortholog(s) have role in mitochondrial translation and mitochondrion localization YDR341C No No Ortholog(s) have role in negative regulation of ubiquitin-protein transferase activity, replicative CAGL0A02024g YOL025W No No cell aging and mitochondrion localization CAGL0A02365g Ortholog(s) have role in cellular response to oxidative stress and cytosol, nucleus localization YDR346C No No Ortholog(s) have 5'-3' exonuclease activity, role in deadenylation-dependent decapping of CAGL0A02860g YDR370C No No nuclear-transcribed mRNA and cytoplasm localization CAGL0A03168g 60S acidic ribosomal protein YDR382W No No Ortholog(s) have IMP cyclohydrolase activity, phosphoribosylaminoimidazolecarboxamide CAGL0A03366g formyltransferase activity, role in 'de novo' IMP biosynthetic process and cytosol, plasma YLR028C Yes No membrane localization Ortholog(s) have role in maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU- CAGL0A03652g rRNA, 5.8S rRNA, LSU-rRNA), maturation of LSU-rRNA from tricistronic rRNA transcript YBR142W No No (SSU-rRNA, 5.8S rRNA, LSU-rRNA) and nucleolus localization CAGL0A04081g Predicted GPI-linked cell wall protein YLR194C No Yes Ortholog(s) have role in mitochondrion inheritance and cellular bud neck, incipient cellular bud CAGL0A04169g YLR190W No Yes site, mitochondrial outer membrane localization CAGL0A04213g Has domain(s) with predicted nucleic acid binding, nucleotide binding activity YBL051C No No Ortholog(s) have thioredoxin peroxidase activity and role in cell redox homeostasis, cellular CAGL0A04433g response to oxidative stress, response to cadmium ion, sporocarp development involved in YBL064C Yes No sexual reproduction CAGL0A04675g Ortholog(s) have protein tag activity YBL078C No Yes Ortholog(s) have protein anchor activity, role in vacuole inheritance and Myo2p-Vac17p-Vac8p CAGL0B00308g YCL063W No No transport complex, fungal-type vacuole membrane localization Has domain(s) with predicted ATP binding, phosphotransferase activity, alcohol group as CAGL0B00726g YCL040W No No acceptor activity and role in carbohydrate metabolic process CAGL0B01100g Phosphatidylethanolamine-binding protein YLR178C Yes Yes Ortholog(s) have chromatin binding activity, role in cellular response to DNA damage stimulus CAGL0B01188g YLR183C No No and Rpd3L-Expanded complex, mitochondrion localization CAGL0B01203g Ortholog(s) have cytosolic large ribosomal subunit localization YDR500C Yes No Ortholog(s) have RNA polymerase II C-terminal domain phosphoserine binding, RNA CAGL0B01375g polymerase II core binding, RNA polymerase II transcription factor binding transcription factor YOL145C Yes No activity, triplex DNA binding activity Ortholog(s) have histone deacetylase activity, transcription coactivator activity, transcription CAGL0B01441g YNL330C Yes No corepressor activity Ortholog(s) have role in cellular copper ion homeostasis, endosomal transport, invasive growth CAGL0B01683g YLR083C No No in response to glucose limitation, pseudohyphal growth, vacuolar transport Ortholog(s) have structural constituent of ribosome activity and mitochondrial large ribosomal CAGL0B01815g YDR116C No No subunit, nucleolus localization Ortholog(s) have tRNA (guanine-N2-)-methyltransferase activity, role in tRNA N2-guanine CAGL0B01881g YDR120C Yes No methylation and mitochondrion, nuclear inner membrane localization Has domain(s) with predicted ATP binding, protein kinase activity, protein tyrosine kinase CAGL0B01925g activity, transferase activity, transferring phosphorus-containing groups activity and role in YDR122W No Yes protein phosphorylation CAGL0B02563g Expressed protein of unknown function YML128C Yes Yes Ortholog(s) have phosphoprotein phosphatase activity, role in endoplasmic reticulum unfolded CAGL0B02761g protein response, protein dephosphorylation, traversing start control point of mitotic cell cycle YLR361C No No and endoplasmic reticulum localization CAGL0K04279g Protein of unknown function YGR049W No No CAGL0B03091g Protein of unknown function YGR041W No Yes Ortholog(s) have role in ethanol metabolic process and mitochondrial inner membrane CAGL0B03465g YLR251W No No localization CAGL0B03575g Ortholog(s) have histone acetyltransferase activity, histone binding activity YEL056W No No CAGL0B04301g Ortholog(s) have cytosol localization YBR028C No No Putative Zn(2)-Cys(6) binuclear cluster transcription factor, involved in transcriptional CAGL0C01199g YLR228C Yes No regulation of ergosterol biosynthesis and uptake Ortholog(s) have guanyl-nucleotide exchange factor activity, translation initiation factor activity CAGL0C01221g YDR211W Yes No and role in regulation of translational initiation Ortholog(s) have cytochrome-c oxidase activity, nitrite reductase (NO-forming) activity, role in CAGL0C01325g mitochondrial electron transport, cytochrome c to oxygen and mitochondrial respiratory chain YNL052W Yes Yes complex IV localization CAGL0C01435g Ortholog(s) have role in ribosome biogenesis and mitochondrion localization YPL183W-A No No CAGL0C01441g Ortholog(s) have role in endocytic recycling and cytosol, endosome, nucleus localization YPL183C No No CAGL0C01485g Ortholog(s) have methylated histone binding, transcription factor binding activity YPL182C No - Ortholog(s) have nascent polypeptide-associated complex binding activity, role in protein CAGL0C01793g targeting to mitochondrion, ribosome localization and integral component of mitochondrial YBR230C No No outer membrane localization Ortholog(s) have glutamate-tRNA ligase activity, mRNA binding activity, role in glutamyl- CAGL0C01947g tRNA aminoacylation and cytosol, methionyl glutamyl tRNA synthetase complex, YGL245W No No mitochondrion, nucleus localization CAGL0C02607g Ortholog(s) have ATP binding, phosphatidylinositol binding activity YLR396C No No Ortholog(s) have cytochrome-c oxidase activity, role in mitochondrial electron transport, CAGL0C02623g cytochrome c to oxygen and mitochondrial respiratory chain complex IV, plasma membrane YLR395C No No localization Putative acyl-CoA:sterol acyltransferase involved in sterol esterification; gene is upregulated in CAGL0C02981g YCR048W No No azole-resistant strain CAGL0C03597g Putative actin-binding protein YCR088W No No Ortholog(s) have alditol:NADP+ 1-oxidoreductase activity, role in D-xylose catabolic process, CAGL0C04543g arabinose catabolic process, cellular response to oxidative stress and cytosol, nucleus YJR096W No Yes localization CAGL0C04741g Cytosolic copper-zinc superoxide dismutase YJR104C No Yes CAGL0C05093g Ortholog(s) have dipeptidyl-peptidase activity and cytoplasm, nucleus localization YOL057W No No CAGL0C05291g Ortholog(s) have cytosol localization YOL066C No No Ortholog(s) have peptide alpha-N-acetyltransferase activity, role in N-terminal protein amino CAGL0C05313g YDL040C No No acid acetylation and NatA complex, cytosolic ribosome, mitochondrion localization Ortholog(s) have sequence-specific DNA binding RNA polymerase II transcription factor CAGL0C05335g YOL067C No No activity, transcription regulatory region DNA binding activity Histone deacetylase; ortholog of S. cerevisiae SIR2; sensor of niacin limitation; regulates gene CAGL0C05357g YOL068C No No expression under niacin-limiting conditions Ortholog(s) have GTPase activator activity, role in regulation of vacuole fusion, non-autophagic, CAGL0C05489g YDL234C No Yes vesicle-mediated
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