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GFI1B Antibody Order 021-34695924 Orders@Ab-Mart.Com Support 400-6123-828 50Ul Support1@Ab-Mart.Com 100 Ul √ √ Web

TD10049 GFI1B Antibody Order 021-34695924 [email protected] Support 400-6123-828 50ul [email protected] 100 uL √ √ Web www.ab-mart.com.cn Description: Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI- KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation. Uniprot:Q5VTD9 Alternative Names： GFI 1B; gfi1b; GFI1B protein; GFI1B_HUMAN; Growth factor independent 1B protein; Growth factor independent 1B transcription repressor; Growth factor independent protein 1B; OTTHUMP00000022443; OTTHUMP00000022444; OTTHUMP000000235527; Potential regulator of CDKN1A; Potential regulator of CDKN1A translocated in CML; Translocated in CML; Zinc finger protein Gfi-1b; Specificity: GFI1B Antibody detects endogenous levels of total GFI1B. Reactivity:Human Source:Rabbit Mol.Wt.: 37kD; 37kDa(Calculated). Storage Condition: Store at -20 °C. Stable for 12 months from date of receipt. Application: WB 1:1000-1:2000；IHC/IF 1:50-1:500.

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Analysis of Chromatin-State Plasticity Identifies Cell-Type–Specific Regulators of H3k27me3 Patterns
Analysis of chromatin-state plasticity identifies cell-type–specific regulators of H3K27me3 patterns Luca Pinelloa,1, Jian Xub,1, Stuart H. Orkinb,c,2, and Guo-Cheng Yuana,2 aDepartment of Biostatistics and Computational Biology, Dana-Farber Cancer Institute and Harvard School of Public Heath, Boston, MA 02215; bDivision of Hematology/Oncology, Boston Children’s Hospital and Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115; and cHoward Hughes Medical Institute, Boston, MA 02115 Contributed by Stuart H. Orkin, December 6, 2013 (sent for review August 13, 2013) Chromatin states are highly cell-type–specific, but the underlying a significant fraction of H3K27me3 is located in distal regions. mechanisms for the establishment and maintenance of their ge- It has been proposed that distal H3K27me3 marks poised nome-wide patterns remain poorly understood. Here we present enhancers, which can be activated through replacing H3K27me3 a computational approach for investigation of chromatin-state by H3K27ac (15, 16). An important question is whether there is plasticity. We applied this approach to investigate an ENCODE a general principle controlling the plastic changes of H3K27me3 ChIP-seq dataset profiling the genome-wide distributions of the patterns across different cell types. H3K27me3 mark in 19 human cell lines. We found that the high To systematically investigate the mechanisms modulating plasticity regions (HPRs) can be divided into two functionally and chromatin-state plasticity, we developed and validated a compu- mechanistically distinct subsets, which correspond to CpG island tational approach to identify distinct lineage-restricted regulators (CGI) proximal or distal regions, respectively.
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Rabbit Mab A
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Gfi1b (NM 008114) Mouse Tagged ORF Clone – MG204861 | Origene
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