Development and Utilization of Human Decidualization Reporter Cell Line Uncovers New Modulators of Female Fertility
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Figure 2S 4 7 A - C 080125 CSCs 080418 CSCs - + IFN-a 48 h + IFN-a 48 h + IFN-a 72 h 6 + IFN-a 72 h 3 5 MRFI 4 2 3 2 1 1 0 0 MHC I MHC II MICA MICB ULBP-1 ULBP-2 ULBP-3 ULBP-4 MHC I MHC II MICA MICB ULBP-1 ULBP-2 ULBP-3 ULBP-4 7 B 13 080125 FBS - D 080418 FBS - + IFN-a 48 h 12 + IFN-a 48 h + IFN-a 72 h + IFN-a 72 h 6 080125 FBS 11 10 5 9 8 4 7 6 3 MRFI 5 4 2 3 2 1 1 0 0 MHC I MHC II MICA MICB ULBP-1 ULBP-2 ULBP-3 ULBP-4 MHC I MHC II MICA MICB ULBP-1 ULBP-2 ULBP-3 ULBP-4 Molecule Molecule FIGURE 4S FIGURE 5S Panel A Panel B FIGURE 6S A B C D Supplemental Results Table 1S. Modulation by IFN-α of APM in GBM CSC and FBS tumor cell lines. Molecule * Cell line IFN-α‡ HLA β2-m# HLA LMP TAP1 TAP2 class II A A HC§ 2 7 10 080125 CSCs - 1∞ (1) 3 (65) 2 (91) 1 (2) 6 (47) 2 (61) 1 (3) 1 (2) 1 (3) + 2 (81) 11 (80) 13 (99) 1 (3) 8 (88) 4 (91) 1 (2) 1 (3) 2 (68) 080125 FBS - 2 (81) 4 (63) 4 (83) 1 (3) 6 (80) 3 (67) 2 (86) 1 (3) 2 (75) + 2 (99) 14 (90) 7 (97) 5 (75) 7 (100) 6 (98) 2 (90) 1 (4) 3 (87) 080418 CSCs - 2 (51) 1 (1) 1 (3) 2 (47) 2 (83) 2 (54) 1 (4) 1 (2) 1 (3) + 2 (81) 3 (76) 5 (75) 2 (50) 2 (83) 3 (71) 1 (3) 2 (87) 1 (2) 080418 FBS - 1 (3) 3 (70) 2 (88) 1 (4) 3 (87) 2 (76) 1 (3) 1 (3) 1 (2) + 2 (78) 7 (98) 5 (99) 2 (94) 5 (100) 3 (100) 1 (4) 2 (100) 1 (2) 070104 CSCs - 1 (2) 1 (3) 1 (3) 2 (78) 1 (3) 1 (2) 1 (3) 1 (3) 1 (2) + 2 (98) 8 (100) 10 (88) 4 (89) 3 (98) 3 (94) 1 (4) 2 (86) 2 (79) * expression of APM molecules was evaluated by intracellular staining and cytofluorimetric analysis; ‡ cells were treatead or not (+/-) for 72 h with 1000 IU/ml of IFN-α; # β-2 microglobulin; § β-2 microglobulin-free HLA-A heavy chain; ∞ values are indicated as ratio between the mean of fluorescence intensity of cells stained with the selected mAb and that of the negative control; bold values indicate significant MRFI (≥ 2). -
Mechanotransduction Signaling in Podocytes from Fluid Flow Shear Stress
Children's Mercy Kansas City SHARE @ Children's Mercy Manuscripts, Articles, Book Chapters and Other Papers 1-1-2018 Mechanotransduction signaling in podocytes from fluid flow shear stress. Tarak Srivastava Children's Mercy Hospital Hongying Dai Children's Mercy Hospital Daniel P. Heruth Children's Mercy Hospital Uri S. Alon Children's Mercy Hospital Robert E. Garola Children's Mercy Hospital See next page for additional authors Follow this and additional works at: https://scholarlyexchange.childrensmercy.org/papers Part of the Animal Structures Commons, Medical Biophysics Commons, Medical Genetics Commons, Nephrology Commons, and the Pathology Commons Recommended Citation Srivastava, T., Dai, H., Heruth, D. P., Alon, U. S., Garola, R. E., Zhou, J., Duncan, R. S., El-Meanawy, A., McCarthy, E. T., Sharma, R., Johnson, M. L., Savin, V. J., Sharma, M. Mechanotransduction signaling in podocytes from fluid flow shear stress. American journal of physiology. Renal physiology 314, 22-22 (2018). This Article is brought to you for free and open access by SHARE @ Children's Mercy. It has been accepted for inclusion in Manuscripts, Articles, Book Chapters and Other Papers by an authorized administrator of SHARE @ Children's Mercy. For more information, please contact [email protected]. Creator(s) Tarak Srivastava, Hongying Dai, Daniel P. Heruth, Uri S. Alon, Robert E. Garola, Jianping Zhou, R Scott Duncan, Ashraf El-Meanawy, Ellen T. McCarthy, Ram Sharma, Mark L. Johnson, Virginia J. Savin, and Mukut Sharma This article is available at SHARE @ Children's Mercy: https://scholarlyexchange.childrensmercy.org/papers/1227 Am J Physiol Renal Physiol 314: F22–F34, 2018. First published September 6, 2017; doi:10.1152/ajprenal.00325.2017. -
Screening and Identification of Key Biomarkers in Clear Cell Renal Cell Carcinoma Based on Bioinformatics Analysis
bioRxiv preprint doi: https://doi.org/10.1101/2020.12.21.423889; this version posted December 23, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Screening and identification of key biomarkers in clear cell renal cell carcinoma based on bioinformatics analysis Basavaraj Vastrad1, Chanabasayya Vastrad*2 , Iranna Kotturshetti 1. Department of Biochemistry, Basaveshwar College of Pharmacy, Gadag, Karnataka 582103, India. 2. Biostatistics and Bioinformatics, Chanabasava Nilaya, Bharthinagar, Dharwad 580001, Karanataka, India. 3. Department of Ayurveda, Rajiv Gandhi Education Society`s Ayurvedic Medical College, Ron, Karnataka 562209, India. * Chanabasayya Vastrad [email protected] Ph: +919480073398 Chanabasava Nilaya, Bharthinagar, Dharwad 580001 , Karanataka, India bioRxiv preprint doi: https://doi.org/10.1101/2020.12.21.423889; this version posted December 23, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract Clear cell renal cell carcinoma (ccRCC) is one of the most common types of malignancy of the urinary system. The pathogenesis and effective diagnosis of ccRCC have become popular topics for research in the previous decade. In the current study, an integrated bioinformatics analysis was performed to identify core genes associated in ccRCC. An expression dataset (GSE105261) was downloaded from the Gene Expression Omnibus database, and included 26 ccRCC and 9 normal kideny samples. Assessment of the microarray dataset led to the recognition of differentially expressed genes (DEGs), which was subsequently used for pathway and gene ontology (GO) enrichment analysis. -
Down-Regulation of Stem Cell Genes, Including Those in a 200-Kb Gene Cluster at 12P13.31, Is Associated with in Vivo Differentiation of Human Male Germ Cell Tumors
Research Article Down-Regulation of Stem Cell Genes, Including Those in a 200-kb Gene Cluster at 12p13.31, Is Associated with In vivo Differentiation of Human Male Germ Cell Tumors James E. Korkola,1 Jane Houldsworth,1,2 Rajendrakumar S.V. Chadalavada,1 Adam B. Olshen,3 Debbie Dobrzynski,2 Victor E. Reuter,4 George J. Bosl,2 and R.S.K. Chaganti1,2 1Cell Biology Program and Departments of 2Medicine, 3Epidemiology and Biostatistics, and 4Pathology, Memorial Sloan-Kettering Cancer Center, New York, New York Abstract on the degree and type of differentiation (i.e., seminomas, which Adult male germ cell tumors (GCTs) comprise distinct groups: resemble undifferentiated primitive germ cells, and nonseminomas, seminomas and nonseminomas, which include pluripotent which show varying degrees of embryonic and extraembryonic embryonal carcinomas as well as other histologic subtypes patterns of differentiation; refs. 2, 3). Nonseminomatous GCTs are exhibiting various stages of differentiation. Almost all GCTs further subdivided into embryonal carcinomas, which show early show 12p gain, but the target genes have not been clearly zygotic or embryonal-like differentiation, yolk sac tumors and defined. To identify 12p target genes, we examined Affymetrix choriocarcinomas, which exhibit extraembryonal forms of differ- (Santa Clara, CA) U133A+B microarray (f83% coverage of 12p entiation, and teratomas, which show somatic differentiation along genes) expression profiles of 17 seminomas, 84 nonseminoma multiple lineages (3). Both seminomas and embryonal carcinoma GCTs, and 5 normal testis samples. Seventy-three genes on 12p are known to express stem cell markers, such as POU5F1 (4) and were significantly overexpressed, including GLUT3 and REA NANOG (5). -
Transcription Factor Creb3l1 Regulates the Synthesis of Prohormone Convertase Enzyme PC1/3 in Endocrine Cells
Greenwood, M., Paterson, A., Rahman, P. A., Gillard, B. T., Langley, S., Iwasaki, Y., Murphy, D., & Greenwood, M. P. (2020). Transcription factor Creb3l1 regulates the synthesis of prohormone convertase enzyme PC1/3 in endocrine cells. Journal of Neuroendocrinology, 32(4), [E12851]. https://doi.org/10.1111/jne.12851 Publisher's PDF, also known as Version of record License (if available): CC BY Link to published version (if available): 10.1111/jne.12851 Link to publication record in Explore Bristol Research PDF-document This is the final published version of the article (version of record). It first appeared online via Wiley at https://onlinelibrary.wiley.com/doi/full/10.1111/jne.12851 . Please refer to any applicable terms of use of the publisher. University of Bristol - Explore Bristol Research General rights This document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/red/research-policy/pure/user-guides/ebr-terms/ Received: 11 November 2019 | Revised: 31 March 2020 | Accepted: 31 March 2020 DOI: 10.1111/jne.12851 ORIGINAL ARTICLE Transcription factor Creb3l1 regulates the synthesis of prohormone convertase enzyme PC1/3 in endocrine cells Mingkwan Greenwood1 | Alex Paterson1 | Parveen Akhter Rahman1 | Benjamin Thomas Gillard1 | Sydney Langley1 | Yasumasa Iwasaki2 | David Murphy1 | Michael Paul Greenwood1 1Translational Health Sciences, Bristol Medical School, University of Bristol, Bristol, Abstract UK Transcription factor cAMP responsive element-binding protein 3 like 1 (Creb3l1) is a 2 Health Care Center, Kochi University, non-classical endoplasmic reticulum stress molecule that is emerging as an important Kochi, Japan component for cellular homeostasis, particularly within cell types with high peptide Correspondence secretory capabilities. -
Interaction Network of Immune‑Associated Genes Affecting the Prognosis of Patients with Glioblastoma
EXPERIMENTAL AND THERAPEUTIC MEDICINE 21: 61, 2021 Interaction network of immune‑associated genes affecting the prognosis of patients with glioblastoma XIAOHONG HOU1*, JIALIN CHEN2*, QIANG ZHANG1, YINCHUN FAN1, CHENGMING XIANG1, GUIYIN ZHOU1, FANG CAO1 and SHENGTAO YAO1 1Department of Cerebrovascular Disease, Affiliated Hospital of Zunyi Medical University;2 Department of Neonatology, The First People' s Hospital of Zunyi Affiliated to Zunyi Medical University, Zunyi, Guizhou 563000, P.R. China Received October 15, 2019; Accepted October 6, 2020 DOI: 10.3892/etm.2020.9493 Abstract. Glioblastoma multiforme (GBM) is a common and immune genes of interest. The interaction network of malignant tumor type of the nervous system. The purpose immune‑regulatory genes constructed in the present study of the present study was to establish a regulatory network of enhances the current understanding of mechanisms associated immune‑associated genes affecting the prognosis of patients with poor prognosis of patients with GBM. The risk score with GBM. The GSE4290, GSE50161 and GSE2223 datasets model established in the present study may be used to evaluate from the Gene Expression Omnibus database were screened the prognosis of patients with GBM. to identify common differentially expressed genes (co‑DEGs). A functional enrichment analysis indicated that the co‑DEGs Introduction were mainly enriched in cell communication, regulation of enzyme activity, immune response, nervous system, cytokine Glioblastoma multiforme (GBM) is one of the most malig‑ signaling in immune system and the AKT signaling pathway. nant tumor types of the central nervous system, with short The co‑DEGs accumulated in immune response were then median survival and poor prognosis. -
Selective Estrogen Receptor Modulators: Discrimination of Agonistic Versus Antagonistic Activities by Gene Expression Profiling in Breast Cancer Cells
[CANCER RESEARCH 64, 1522–1533, February 15, 2004] Selective Estrogen Receptor Modulators: Discrimination of Agonistic versus Antagonistic Activities by Gene Expression Profiling in Breast Cancer Cells Jonna Frasor,1 Fabio Stossi,1 Jeanne M. Danes,1 Barry Komm,2 C. Richard Lyttle,2 and Benita S. Katzenellenbogen1 1Department of Molecular and Integrative Physiology, University of Illinois and College of Medicine, Urbana, Illinois, and 2Women’s Health Research Institute, Wyeth Research, Collegeville, Pennsylvania ABSTRACT tures in these women; however, some detrimental side effects such as an increased risk of endometrial cancer, stroke, and pulmonary embolism Selective estrogen receptor modulators (SERMs) such as tamoxifen are were also associated with tamoxifen treatment (7). Ral was examined in effective in the treatment of many estrogen receptor-positive breast cancers the Multiple Outcomes of Raloxifene Evaluation trial and found to be and have also proven to be effective in the prevention of breast cancer in women at high risk for the disease. The comparative abilities of tamoxifen effective in reducing the incidence of osteoporosis in postmenopausal versus raloxifene in breast cancer prevention are currently being compared in women, as well as the incidence of breast cancer but, unlike tamoxifen, the Study of Tamoxifen and Raloxifene trial. To better understand the actions without the increased risk of endometrial cancer (8, 9). On the basis of the of these compounds in breast cancer, we have examined their effects on the positive outcome of these trials, the Study of Tamoxifen and Raloxifene expression of ϳ12,000 genes, using Affymetrix GeneChip microarrays, with trial was begun in 1999 to directly compare the effects of these two quantitative PCR verification in many cases, categorizing their actions as SERMs, tamoxifen and Ral, in prevention of breast cancer (10, 11). -
Genomic Alterations of Ground-Glass Nodular Lung Adenocarcinoma
www.nature.com/scientificreports OPEN Genomic alterations of ground- glass nodular lung adenocarcinoma Hyun Lee1, Je-Gun Joung2, Hyun-Tae Shin2, Duk-Hwan Kim3, Yujin Kim3, Hojoong Kim1, O. Jung Kwon1, Young Mog Shim4, Ho Yun Lee5, Kyung Soo Lee5, Yoon-La Choi6, 2 7 1 Received: 1 February 2018 Woong-Yang Park , D. Neil Hayes & Sang-Won Um Accepted: 30 April 2018 In-depth molecular pathogenesis of ground-glass nodular lung adenocarcinoma has not been well Published: xx xx xxxx understood. The objectives of this study were to identify genomic alterations in ground-glass nodular lung adenocarcinomas and to investigate whether viral transcripts were detected in these tumors. Nine patients with pure (n = 4) and part-solid (n = 5) ground-glass nodular adenocarcinomas were included. Six were females with a median age of 58 years. We performed targeted exon sequencing and RNA sequencing. EGFR (n = 10), IDH2 (n = 2), TP53 (n = 1), PTEN (n = 1), EPHB4 (n = 1), and BRAF (n = 1) were identifed as driver mutations by targeted exon sequencing. Vasculogenesis-associated genes including NOTCH4 and TGFBR3 expression were signifcantly downregulated in adenocarcinoma tissue versus normal tissue (adjusted P values < 0.001 for both NOTCH4 and TGFBR3). In addition, fve novel fusion gene loci were identifed in four lung adenocarcinomas. However, no signifcant virus-associated transcripts were detected in tumors. In conclusions, EGFR, IDH2, TP53, PTEN, EPHB4, and BRAF were identifed as putative driver mutations of ground-glass nodular adenocarcinomas. Five novel fusion genes were also identifed in four tumors. Viruses do not appear to be involved in the tumorigenesis of ground-glass nodular lung adenocarcinoma. -
Differential Gene Expression of Minnesota (MN) Hygienic Honeybees (Apis Mellifera) Performing Hygienic Behavior
Minnesota State University, Mankato Cornerstone: A Collection of Scholarly and Creative Works for Minnesota State University, Mankato All Graduate Theses, Dissertations, and Other Graduate Theses, Dissertations, and Other Capstone Projects Capstone Projects 2016 Differential Gene Expression of Minnesota (MN) Hygienic Honeybees (Apis mellifera) Performing Hygienic Behavior Eric Northrup Minnesota State University Mankato Follow this and additional works at: https://cornerstone.lib.mnsu.edu/etds Part of the Bioinformatics Commons, Entomology Commons, and the Genetics Commons Recommended Citation Northrup, E. (2016). Differential Gene Expression of Minnesota (MN) Hygienic Honeybees (Apis mellifera) Performing Hygienic Behavior [Master’s thesis, Minnesota State University, Mankato]. Cornerstone: A Collection of Scholarly and Creative Works for Minnesota State University, Mankato. https://cornerstone.lib.mnsu.edu/etds/619/ This Thesis is brought to you for free and open access by the Graduate Theses, Dissertations, and Other Capstone Projects at Cornerstone: A Collection of Scholarly and Creative Works for Minnesota State University, Mankato. It has been accepted for inclusion in All Graduate Theses, Dissertations, and Other Capstone Projects by an authorized administrator of Cornerstone: A Collection of Scholarly and Creative Works for Minnesota State University, Mankato. Differential gene expression of Minnesota (MN) Hygienic honeybees (Apis mellifera) performing hygienic behavior By Eric Northrup A Thesis Submitted in Partial Fulfillment of the Requirements for the Degree of Masters of Science In Biological Sciences Minnesota State University, Mankato Mankato, Minnesota April 2016 Differential gene expression of MN Hygienic honeybees (Apis mellifera) performing hygienic behavior Eric Northrup This thesis has been examined and approved by the following members of the student’s committee. -
Prospective Isolation of NKX2-1–Expressing Human Lung Progenitors Derived from Pluripotent Stem Cells
The Journal of Clinical Investigation RESEARCH ARTICLE Prospective isolation of NKX2-1–expressing human lung progenitors derived from pluripotent stem cells Finn Hawkins,1,2 Philipp Kramer,3 Anjali Jacob,1,2 Ian Driver,4 Dylan C. Thomas,1 Katherine B. McCauley,1,2 Nicholas Skvir,1 Ana M. Crane,3 Anita A. Kurmann,1,5 Anthony N. Hollenberg,5 Sinead Nguyen,1 Brandon G. Wong,6 Ahmad S. Khalil,6,7 Sarah X.L. Huang,3,8 Susan Guttentag,9 Jason R. Rock,4 John M. Shannon,10 Brian R. Davis,3 and Darrell N. Kotton1,2 2 1Center for Regenerative Medicine, and The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA. 3Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center, Houston, Texas, USA. 4Department of Anatomy, UCSF, San Francisco, California, USA. 5Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA. 6Department of Biomedical Engineering and Biological Design Center, Boston University, Boston, Massachusetts, USA. 7Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA. 8Columbia Center for Translational Immunology & Columbia Center for Human Development, Columbia University Medical Center, New York, New York, USA. 9Department of Pediatrics, Monroe Carell Jr. Children’s Hospital, Vanderbilt University, Nashville, Tennessee, USA. 10Division of Pulmonary Biology, Cincinnati Children’s Hospital, Cincinnati, Ohio, USA. It has been postulated that during human fetal development, all cells of the lung epithelium derive from embryonic, endodermal, NK2 homeobox 1–expressing (NKX2-1+) precursor cells. -
Mediator of DNA Damage Checkpoint 1 (MDC1) Is a Novel Estrogen Receptor Co-Regulator in Invasive 6 Lobular Carcinoma of the Breast 7 8 Evelyn K
bioRxiv preprint doi: https://doi.org/10.1101/2020.12.16.423142; this version posted December 16, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 1 Running Title: MDC1 co-regulates ER in ILC 2 3 Research article 4 5 Mediator of DNA damage checkpoint 1 (MDC1) is a novel estrogen receptor co-regulator in invasive 6 lobular carcinoma of the breast 7 8 Evelyn K. Bordeaux1+, Joseph L. Sottnik1+, Sanjana Mehrotra1, Sarah E. Ferrara2, Andrew E. Goodspeed2,3, James 9 C. Costello2,3, Matthew J. Sikora1 10 11 +EKB and JLS contributed equally to this project. 12 13 Affiliations 14 1Dept. of Pathology, University of Colorado Anschutz Medical Campus 15 2Biostatistics and Bioinformatics Shared Resource, University of Colorado Comprehensive Cancer Center 16 3Dept. of Pharmacology, University of Colorado Anschutz Medical Campus 17 18 Corresponding author 19 Matthew J. Sikora, PhD.; Mail Stop 8104, Research Complex 1 South, Room 5117, 12801 E. 17th Ave.; Aurora, 20 CO 80045. Tel: (303)724-4301; Fax: (303)724-3712; email: [email protected]. Twitter: 21 @mjsikora 22 23 Authors' contributions 24 MJS conceived of the project. MJS, EKB, and JLS designed and performed experiments. JLS developed models 25 for the project. EKB, JLS, SM, and AEG contributed to data analysis and interpretation. SEF, AEG, and JCC 26 developed and performed informatics analyses. MJS wrote the draft manuscript; all authors read and revised the 27 manuscript and have read and approved of this version of the manuscript. -
CREB3L1 Antibody (C-Term) Purified Rabbit Polyclonal Antibody (Pab) Catalog # Ap6589b
10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 CREB3L1 Antibody (C-term) Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP6589b Specification CREB3L1 Antibody (C-term) - Product Information Application WB, IHC-P, FC,E Primary Accession Q96BA8 Other Accession NP_443086 Reactivity Human, Mouse Host Rabbit Clonality Polyclonal Isotype Rabbit Ig Calculated MW 57005 Antigen Region 481-509 CREB3L1 Antibody (C-term) - Additional Information Western blot analysis of CREB3L1 Antibody Gene ID 90993 (C-term) (Cat. #AP6589b) in mouse stomach tissue lysates (35ug/lane). CREB3L1 (arrow) Other Names Cyclic AMP-responsive element-binding was detected using the purified Pab. protein 3-like protein 1, cAMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance, OASIS, Processed cyclic AMP-responsive element-binding protein 3-like protein 1, CREB3L1, OASIS Target/Specificity This CREB3L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 481-509 amino acids from the C-terminal region of human CREB3L1. Dilution WB~~1:8000 IHC-P~~1:50~100 FC~~1:10~50 Anti-CREB3L1 Antibody (C-term) at 1:8000 Format dilution + HepG2 whole cell lysate Purified polyclonal antibody supplied in PBS Lysates/proteins at 20 µg per lane. with 0.09% (W/V) sodium azide. This Secondary Goat Anti-Rabbit IgG, (H+L), antibody is prepared by Saturated Peroxidase conjugated at 1/10000 dilution. Ammonium Sulfate (SAS) precipitation Predicted band size : 57 kDa followed by dialysis against PBS. Blocking/Dilution buffer: 5% NFDM/TBST. Storage Maintain refrigerated at 2-8°C for up to 2 Page 1/3 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 weeks.