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Genetics and Genomics of Alcohol Sensitivity

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Genetics and Genomics of Alcohol Sensitivity Mol Genet Genomics DOI 10.1007/s00438-013-0808-y REVIEW Genetics and genomics of alcohol sensitivity Tatiana V. Morozova · Trudy F. C. Mackay · Robert R. H. Anholt Received: 4 November 2013 / Accepted: 22 December 2013 © The Author(s) 2014. This article is published with open access at Springerlink.com Abstract Alcohol abuse and alcoholism incur a heavy Introduction socioeconomic cost in many countries. Both genetic and environmental factors contribute to variation in the inebri- Alcoholic beverages have been around since time imme- ating effects of alcohol and alcohol addiction among indi- morial and have served economic, social, medical and viduals within and across populations. From a genetics religious purposes. Alcohol is unique among substance perspective, alcohol sensitivity is a quantitative trait deter- abuse drugs, as it is a natural by-product of fermentation. mined by the cumulative effects of multiple segregating Moderate drinking of alcohol may offer health benefits genes and their interactions with the environment. This (Marugame et al. 2007), including reduction in cardiovas- review summarizes insights from model organisms as well cular disease (Baer et al. 2002), ischemic strokes (Zeng as human populations that represent our current under- et al. 2012; Peng et al. 2013), stress levels, incidence of standing of the genetic and genomic underpinnings that type II diabetes (Conigrave et al. 2001; Koppes et al. 2005) govern alcohol metabolism and the sedative and addictive and gallstone disease (Leitzmann et al. 1999). Exces- effects of alcohol on the nervous system. sive alcohol consumption, however, is a common cause of preventable death in most countries, and imposes a major Keywords Addiction · Behavioral genetics · Genome- socioeconomic burden. Alcohol abuse and dependence dis- wide association · Quantitative trait loci · Meta-analysis orders are associated with marital instability, violent crime, fatal accidents, and injuries (Chick 2011). Heavy drinking is associated with increased risk of different types of can- “Alcohol is the anaesthesia by which we endure the opera- cer (Nelson et al. 2013; Touvier et al. 2013), higher cardio- tion of life”. vascular disease mortality (Graff-Iversen et al. 2013), birth George Bernard Shaw defects (Feldman et al. 2012), liver diseases (McCullough et al. 2011), and neuropsychiatric disorders (Rivas et al. 2013). Effects of alcohol range from sedation, characterized by decreased awareness and ability to function, to addic- tion. The term “alcoholic” was introduced by the physician Communicated by J. Graw. Magnus Huss in 1849 to describe alcohol addiction and can be defined as persistence of excessive drinking over a long Electronic supplementary material The online version of this period of time despite adverse health effects and disrup- article (doi:10.1007/s00438-013-0808-y) contains supplementary material, which is available to authorized users. tion of social relations. Not all individuals who consume alcohol become alcoholics. Vulnerability to develop addic- T. V. Morozova · T. F. C. Mackay · R. R. H. Anholt (*) tion depends on genetic, physiological and environmen- Department of Biological Sciences and W. M. Keck Center tal conditions. Sustained alcohol intake can lead to func- for Behavioral Biology, North Carolina State University, Box 7617, Raleigh, NC 27695-7617, USA tional alcohol tolerance, which enables increased alcohol e-mail: [email protected] consumption with fewer signs of intoxication. Alcohol 1 3 Mol Genet Genomics tolerance allows escalation of drinking and eventually association studies (GWAS), have implicated many vari- development of addiction. Alcohol “dependence” generally ants in and around the seven ADH genes that contribute refers to physiological addiction, when cessation of alcohol to alcohol dependence or alcohol-related traits, including intake precipitates withdrawal reactions, which range from alleles of ADH1A, ADH1B, ADH1C, ADH2 and ADH4. anxiety and shakiness to severe complications, such as sei- While there are 18 genes encoding members of the ALDH zures and delirium tremens. The term “alcohol preference” enzyme family, only ALDH2 plays a major role in oxidiz- refers to selectively bred strains of laboratory rats and mice ing acetaldehyde in the liver (Edenberg 2013). that either prefer or avoid alcohol consumption. Alcohol Alcoholism is less common in East Asian and Poly- preferring rodents voluntarily consume greater amounts of nesian populations than in European populations, due to alcohol than non-preferring animals and have been studied protective ADH and ALDH alleles. A variant of ADH1B extensively as models for alcohol addiction in humans. (ADH1B*2; Arg47His), which occurs primarily in Asian Sensitivity to alcohol exposure varies among individuals and Polynesian populations, shows 100-fold higher enzy- within and across populations. From a genetics perspective, matic activity (Thomasson et al. 1995; Mulligan et al. susceptibility to the inebriating effects of alcohol and alco- 2003; Birley et al. 2009; Bierut et al. 2012; Hurley and hol addiction can be viewed as quantitative traits that result Edenberg 2012), thereby attenuating the development of from the cumulative effects of multiple segregating genes tolerance. Furthermore, the ALDH2*2 allele (Glu504Lys), and their interactions with the environment. Thus, there which is also common in East Asia, is associated with aver- is no single locus that predisposes to alcohol abuse and sive reactions to alcohol consumption, such as facial flush- dependence disorders, but rather many variants and their ing, hypotension, headaches and nausea (Edenberg 2007). interactions with each other and the environment under- Polymorphisms in two other enzymes, ALDH1A1 (Lind lie alcohol-related phenotypes (Ducci and Goldman 2008; et al. 2008) and ALDH1B1 (Linneberg et al. 2010), have Morozova et al. 2012; Edenberg 2013). Disentangling the also been associated with alcohol consumption in Finnish genetic and environmental contributions that shape alcohol- and Danish populations, respectively. related phenotypes is complicated, because in human popu- Studies on the Wistar-derived UChB line of rats, which lations neither the genetic background nor the environment were bred for high ethanol intake, replicated the protec- can be controlled precisely, results of excessive alcohol tive ALDH2 phenotype observed in Asian populations fol- consumption are diverse, ranging from sedation to addic- lowing intravenous injection of an adenoviral vector with tion and often confounded by neuropsychiatric conditions, an Aldh2 antisense gene. This led to an 85 % decrease in and different studies have utilized different measurements ALDH2 activity in the liver and inhibited voluntary ethanol to document alcohol-related phenotypes, such as frequency consumption up to 50 % (Ocaranza et al. 2008). Further- of drinks, total amount of alcohol consumed per drinking more, studies with an adenoviral vector containing a mul- session, age at first drink, and withdrawal symptoms. This tiple expression cassette showed that simultaneous increase review integrates insights obtained from different model of ADH and decrease of ALDH2 activities in the liver dra- systems as well as human population studies to provide a matically reduced voluntarily ethanol consumption of alco- comprehensive overview of the genetic factors that mediate hol-dependent animals (Rivera-Meza et al. 2012). sensitivity to alcohol. Additional insights in the metabolism of alcohol come from studies on the fruit fly Drosophila melanogaster. Fruit flies encounter ethanol in their natural habitat, since larvae Alcohol metabolism feed on fermented food sources, which provide substrates for lipid synthesis (Geer et al. 1985). They show preference The rate at which alcohol is metabolized and the nature for ethanol containing food over non ethanol containing and fate of its degradation products are important factors food in laboratory experiments because of the caloric value that determine its physiological effects. The primary path- of ethanol (Pohl et al. 2012). Both Adh and Aldh contrib- way of ethanol metabolism in liver, and to a lesser extent ute to ethanol resistance in Drosophila (Fry and Saweikis in the stomach and intestinal tract, involves conversion of 2006). There are two Adh alleles, designated Slow (AdhS) ethanol to acetaldehyde by alcohol dehydrogenase (ADH; and Fast (AdhF) based on their electrophoretic mobility, Fig. 1), with acetaldehyde playing a major role in mediat- that differ by a single amino acid (McDonald et al. 1980). ing aversive and rewarding effects of ethanol. Acetaldehyde Fast homozygotes have a higher level of enzymatic activ- is oxidized further to acetate by aldehyde dehydrogenase ity than Slow homozygotes and higher tolerance to alco- (ALDH; Fig. 1). The human genome contains five ADH hol in laboratory toxicity tests (McKenzie and McKechnie classes with a total of seven closely related genes located 1978). The physiological and behavioral aspects of ethanol on chromosome 4q. Candidate gene approaches, as well as in D. melanogaster have been reviewed (Kaun et al. 2012; family-based linkage studies together with genome-wide Devineni and Heberlein 2013). 1 3 Mol Genet Genomics ENERGY STORAGE fatty acids ethanol NADPH alcohol dehydrogenase malonyl-CoA acetaldehyde aldehyde pyruvate dehydrogenase malic enzyme acetyl-CoA acetate NADP oxaloacetate citrate malate isocitrate tricarboxylic acid cycle fumarate succinate ENERGY PRODUCTION Fig.
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