2019 Photosynthetic Systems Principal Investigators Meeting Marriott Washingtonian Center, Gaithersburg, MD October 21 to 23, 2019
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2019 Photosynthetic Systems Principal Investigators Meeting Marriott Washingtonian Center, Gaithersburg, MD October 21 to 23, 2019 A C B E D Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences & Biosciences Cover Art Cover art is taken from the abstracts of meeting participants: A: Jessica Anna, B: Christine Kirmaier, C: David Britt, D: Megan Thielges, E: Jan Kern Acknowledgement and Disclaimer The research described herein is, unless specifically labeled otherwise, supported by the Chemical Sciences, Geosciences, and Biosciences Division of the Office of Basic Energy Sciences, U.S. Department of Energy. This document is therefore a compilation of accounts of work sponsored by an agency of the United States Government. Neither the United States government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise, does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof. Foreword This volume is a record of the 6th biennial meeting of the principal investigators funded by Photosynthetic Systems, a program of the Chemical Sciences, Geosciences, and Biosciences (CSGB) Division of the Office of Basic Energy Sciences (BES), U.S. Department of Energy (DOE). CSGB supports basic biochemistry research relevant to energy technology through Photosynthetic Systems and Physical Biosciences, two complementary programs established in 2007. These programs, along with the Solar Photochemistry program, comprise the CSGB Photochemistry and Biochemistry Team, a coordinated group of programs supporting areas of basic research that have been central to the science mission of the DOE since its inception. The abstracts in this volume describe research at the leading edge of understanding natural photosynthesis, a process that exhibits great structural and chemical diversity across the biological world but that uniformly captures and stores solar energy with unmatched efficiency. The high caliber of the research in these abstracts reflects the talent, dedication, and industry of the principal investigators who make Photosynthetic Systems the vibrant and innovative science funding program that it is. The purpose of this meeting is to report recent research accomplishments and foster exchange of scientific knowledge and insights among all participants. Accordingly, the meeting is designed to promote sharing of new results and methodologies, facilitate cooperation and collaboration, challenge old paradigms with new, and provide opportunities to interact with program managers and staff of the DOE. In keeping with this purpose, questions and ideas from meeting participants are welcome at all times. We thank Teresa Crockett of BES and Connie Lansdon of the Oak Ridge Institute for Science and Education (ORISE) for their competent planning and successful execution of meeting logistics. Without their help, the meeting would not have occurred. Stephen K. Herbert, Program Manager, Photosynthetic Systems, DOE BES Robert J. Stack, Program Manager, Physical Biosciences, DOE BES B. Gail Mclean, Team Lead, Photochemistry and Biochemistry Team, DOE BES Links to Sections (Click on the links below to go to sections of this document) Agenda Posters Abstracts Participant List 2019 Photosynthetic Systems Principal Investigators Meeting Gaithersburg Marriott, Washingtonian Center, Gaithersburg, MD. October 21 to 23 AGENDA Monday, October 21 7:30 – 8:30AM Continental Breakfast 8:30 – 9:00AM Overview: Chemical Sciences, Geosciences, and Biosciences Division Bruce Garrett, Division Director, Office of Basic Energy Sciences Section I: Light Harvesting Moderator: Warren Beck, Michigan State University 09:00 –9:30AM Tracking Photochemical and Photophysical Processes of Photosystem I via Multidimensional Electronic and Vibrational Spectroscopic Methods Jessica Anna, University of Pennsylvania 09:30 – 10:00AM Identifying the Energy Transfer Dynamics of Light-Harvesting Complexes in Membrane Nanodiscs Gabriela Schlau-Cohen, Massachusetts Institute of Technology 10:00 – 10:30AM Revealing Excitonic Structure and Charge Transfer in Photosynthetic Proteins by Time-Resolved Circular Dichroism Spectroscopy Sergei Savikhin, Purdue University 10:30 – 11:00AM Coffee Break 11:00 – 11:30AM Multidimensional Spectroscopies for Probing Coherence and Charge Separation in Photosystem II Jennifer Ogilvie, University of Michigan 11:30 – 12:10PM Regulation of Photosynthetic Light Harvesting Graham Fleming, U. California, Berkeley, Lawrence Berkeley Nat’l Lab Kris Niyogi, U. California, Berkeley & Lawrence Berkeley Nat’l Lab Section II: Electron and Proton Transport Moderator: Marilyn Gunner, City U. of New York 12:10 – 12:40PM Visualizing Structural Dynamics in Photosynthetic Reaction Centers on the Femtosecond to Seconds Time Scale Jan Kern, Lawrence Berkeley National Lab 12:40 – 03:00PM Lunch and Afternoon Break Monday, October 21, continued 03:00 – 3:30PM High Field EPR Studies of the Spin=3 S3 State of the OEC and an Analogous Mn(IV)4 Model Complex David Britt, University of California, Davis 03:30 – 04:00PM FTIR Studies of Photosynthetic Oxygen Production Rick Debus, University of California, Riverside 04:00 –04:30PM Insights into Proton-Transfer Pathways During Water Oxidation in Photosystem II Gary Brudvig, Yale University 04:30 – 05:10PM Controlling Electron Transport Pathways in Photosynthetic Reaction Centers Christine Kirmaier, Washington University Philip Laible, Argonne National Lab 05:10 – 05:40PM Performance and Regulation of Photosystem II Energy Conversion in Oxygenic Phototrophs: From PSII Crystals to Living Cells Chuck Dismukes, Rutgers University 05:40 – 07:30PM Program Dinner 07:30 – 10:00PM Poster Session 1 (Odd numbered posters will be presented. Presenters and titles are listed at end of the agenda. Refreshments may be purchased at the hotel bar.) Tuesday, October 22 07:30 – 08:30AM Continental Breakfast Section II: Electron Transport, continued Moderator: Himadri Pakrasi, Washington University 08:30 – 09:00AM Photodamage and Repair in Higher Plant Photosynthesis Terry Bricker, Louisiana State University 09:00 – 9:30AM Structure-Function of the Cytochrome b6f Lipoprotein Complex Bill Cramer, Purdue University 9:30 – 10:00AM Time-Resolved Step-Scan FTIR Difference Spectroscopy for the Study of PS I with Different Quinones Incorporated into the A1 Binding Site. Gary Hastings, Georgia State University Tuesday, October 22, cont’d 10:00 – 10:30AM The Type I Homodimeric Reaction Center in Heliobacterium modesticaldum Kevin Redding, Arizona State University 10:30 – 11:00AM Coffee Break 11:00 – 11:30AM Structure of the NDH: The Complex I-like Molecule of Photosynthesis Karen Davies, Lawrence Berkeley National Lab 11:30 – 12:00PM Cooperative Control of the O2 Reduction Reaction by Photosynthetic Flavodiiron Proteins Katherine Brown, National Renewable Energy Lab 12:00 – 12:30PM Tracking Sites for Multiscale Photosynthetic Function Using X-ray Scattering and Electron Microscopy Imaging Techniques David Tiede, Argonne National Lab 12:30 – 03:00PM Lunch and Afternoon Break Section II: Electron Transport, continued Moderator: Cara Lubner, National Renewable Energy Lab 03:00 – 03:30PM Electron Valves and a Novel Molecular Switch Controls Electron Flow across Cytochrome b6f to Protect PS I from Photoinhibition Arthur Grossman, Carnegie Institution of Washington 03:30 – 04:00PM Regulation of Sustained Cyclic Electron Flow (CEF) in the Photopsychrophile Chlamydomonas sp. UWO241 Rachel Morgan-Kiss, Miami University 04:00 – 04:30PM MSU-DOE Plant Research Laboratory: Subproject A, Robust Photosynthesis in Dynamic Environments David Kramer, Michigan State University 04:30 – 05:00PM Photosynthetic Reduction of Carbon Dioxide: Biochemical Analysis of Rubisco, Phosphoenolpyruvate Carboxylase and Carbonic Anhydrase. Asaph Cousins, Washington State University 05:00 – 05:30PM MSU-DOE Plant Research Laboratory: Subproject C, Characterizing and Engineering Subcellular and Cellular Modules for Photosynthetic Productivity Cheryl Kerfeld, Michigan State University 05:30 – 07:30PM Dinner on your own Tuesday, October 22, cont’d 07:30 – 10:00PM Poster Session 2 (Even numbered posters will be presented. Presenters and titles are listed at the end of the agenda. Refreshments may be purchased at the hotel bar.) Wednesday, October 23 7:30 – 8:30AM Continental Breakfast Section III: Self Assembly Moderator: Christoph Benning, Plant Research Laboratory, Michigan State University 08:30 –9:00AM Membrane-attached Electron Carriers in Photosynthetic and Respiration: Biogenesis of a Cytochrome Complex from a Facultative Photosynthetic Bacterium Fevzi Daldal, University of Pennsylvania 09:00 – 09:30AM Photophosphorylation Requires Thiol-Disulfide Transactions at the Thylakoid Membrane Patrice Hamel, The Ohio State University 09:30 – 10:00AM MSU-DOE Plant Research Laboratory: Subproject B, Integrating Energy Supply and Demand in the Biological Solar Panel Gregg Howe, Michigan State University