JOURNAL OF BACTERIOLOGY

VOLUME 136 0 NUMBER 3 0 DECEMBER 1978 EDITORIAL BOARD Simon Silver, Editor-in-Chief (1982) Washington University, St. Louis, Mo. Stanley C. Holt, Editor (1982) Donald P. Nierlich, Editor (1982) University ofMassachusetts, Amherst University of California, Los Angeles Sam Kaplan, Editor (1983) Allen T. Phillips, Editor (1980) University ofIllinois, Urbana Pennsylvania State University, University Park, Pa. Elizabeth McFall, Editor (1980) Howard V. Rickenberg, Editor (1983) New York University, New York, N. Y. National Jewish Hospital, Denver, CO Mark Achtman (1979) Wolfgang Epstein (1978) Eugene W. Nester (1978) James N. Adams (1979) David P. Fan (1978) Hiroshi Nikaido (1981) Nina Agabian (1980) Walton L. Fangman (1978) John H. Nordin (1979) James Akagi (1979) Gerald R. Fink (1978) Michio Oishi (1980) David Apirion (1979) W. R. Finnerty (1979) Ronald H. Olsen (1978) Arthur 1. Aronson (1979) John D. Foulds (1979) Sunil Palchaudhuri (1979) Gad Avigad (1980) Bijan K. Ghosh (1981) Charles Panos (1978) Stephen D. Barbour (1979) Harry E. Gilleland, Jr. (1979) Leo Parks (1979) Jeffrey M. Becker (1980) Helen Greer (1980) Martin Pato (1978) Claire M. Berg (1980) Walter R. Guild (1978) William S. Reznikoff (1979) Douglas E. Berg (1980) Tadayo Hashimoto (1979) Palmer Rogers (1978) Richard S. Berk (1980) Gerald L. Hazelbauer (1978) Antonio H. Romano (1979) Harriet Bernheimer (1980) George Hegeman (1980) Barry P. Rosen (1980) Edwin Boatman (1980) Joy Hochstadt (1980) Robert Rownd (1980) Winfried Boos (1979) Bruce Holloway (1979) Harold L. Sadoff (1980) H. D. Braymer (1979) Philip Hylemon (1979) Milton H. Saier, Jr. (1979) Jean Brenchley (1980) Joseph Inselburg (1978) Gene A. Scarborough (1979) George H. Brownell (1980) Robert J. Kadner (1979) Irwin H. Segel (1979) Richard 0. Burns (1980) Donald L. Keister (1980) Jane K. Setlow (1978) A. M. Chakrabarty (1980) Jost Kemper (1978) J. A. Shapiro (1979) Peter J. Chapman (1979) David E. Kennell (1980) David Sherratt (1980) G. William Claus (1979) Bruce C. Kline (1980) Douglas W. Smith (1980) Don B. Clewell (1980) Thomas G. Lessie (1979) Issar Smith (1978) Stanley N. Cohen (1979) Robert L. Lester (1978) Lucile Smith (1978) Ronald S. Cole (1980) Hillel S. Levinson (1978) Dieter G. Soil (1979) S. F. Conti (1978) K. Brooks Low (1979) A. L. Sonenshein (1980) Stephen Cooper (1978) Calvin S. McLaughlin (1978) Dwight Stinnett (1979) John W. Costerton (1980) P. T. Magee (1980) Stanley Streicher (1980) John E. Cronan, Jr. (1980) Michael H. Malamy (1978) Robert L. Switzer (1979) Lolita Daneo-Moore (1981) Manley Mandel (1978) Bonnie M. Tyler (1978) Dana L. Diedrich (1980) Robert E. Marquis (1979) James A. Wechsler (1978) Walter J. Dobrogosz (1979) Neil H. Mendelson (1978) David White (1980) Patrick R. Dugan (1980) Sally Ann Meyer (1981) Clifford A. Woolfolk (1979) A. Eisenstark (1979) Gene E. Michaels (1980) Howard Zalkin (1979) Alan D. Elbein (1979) Robert A. Day, Managing Editor Giselia Pollock, Assistant Managing Editor Linda IiHg, Production Editor 1913 I St., N. W, Washington, D.C. 20006 EX OFFICIO Edwin H. Lennette, President (1978-1979) Willis A. Wood, President-Elect (1978-1979) J. Mehsen Joseph, Secretary Brinton M. Miller, Treasurer The Journal of Bacteriology, a publication of the American Made in the United States of America. Society for Microbiology, 1913 I St., N.W., Washington, D.C. Copyright i) 1978, American Society for Microbiology. 20006, is devoted to the advancement and dissemination of All Rights Reserved. fundamental knowledge concerning bacteria and other microor- ganisms. Instructions to Authors are published in the January issue each year. The Journal is published monthly, and the twelve numbers are divided into four volumes per year. The The code at the top of the first page ofan article in thisjournal nonmember subscription price is $115 per year. Single copies indicates the copyright owner's consent that copies of the article are $10. The member subscription price is $22 per year. Corre- may be made for personal use, or for personal use of specific spondence relating to subscriptions, reprints, defective copies, clients. This consent is given on the condition, however, that the availability of back issues, lost or late proofs, disposition of copier pay the stated per-copy fee through the Copyright Clear- submitted manuscripts, and general editorial matters should be ance Center, Inc., P.O. Box 765, Schenectady, New York 12301, directed to the ASM Publications Office, 1913 I St., N.W., for copying beyond that permitted by Sections 107 or 108 of the Washington, D.C. 20006 (area 202 833-9680). U.S. Copyright Law. This consent does not extend to other kinds ofcopying, such as copying for general distribution, for advertis- Second class postage paid at Washington, D.C. 20006, and at ing or promotional purposes, for creating new collective works, additional mailing offices. or for resale. Author Index Akagi, J. M., 916 Hedges, R.W., 1159 Nester, Eugene W., 909 Ames, Giovanna Ferro-Luzzi, Hennen, Paula E., 929 Nunn, William D., 929 1094 Henning, Ulf, 1189 Anderson, A. J., 886 Hespell, Robert B., 936 Odelson, D. A., 936 Archibald, A. R., 886 Hindennach, Ingrid, 1189 Argast, Manfred, 1070 Hirochika, Hirohiko, 983 Palacios, Rafael, 880 Arnon, Daniel I., 1018 Hofnung, M., 1109 Pan, Pauline, 955 Pollack, Jordan H., 1120 Bacha, P., 1135 Iwaya, Makoto, 1143 Postle, Kathleen, 1165 Becker, J. M., 1174 Biek, Donald P., 1094 Jacob, A. E., 1159 Quinto, Carmen, 880 Blumenthal, Harold J., 1120 Jacoby, G. A., 1159 Boonstra, Johannes, 844 Jinks, David C., 1027 Reznikoff, William S., 1165 Boos, Winfried, 1070 Riazi, Jalil, 1008 Boyen, A., 1187 Kadner, Robert J., 1050 Romano, Nino, 833 Kang, Shyue yue, 867 Rose, B., 1174 Camargo, E. Plessmann, 1184 Kinscherf, Thomas G., 1084 Campomanes, Miguel, 880 Kobayashi, Yasuo, 983 Sabath, L. D., 976 Carithers, Robert P., 1018 Koch, Arthur L., 969 Sanchez, Federico, 880 Carter, Harry B., 929 Kodaira, Ryoji, 947 Schafer, Dorothy, 874 Chilton, Mary-Dell, 1178 Konings, Wil N., 844 Schottel, Janet, 1084 Coulton, J. W., 1037 Korfhagen, T. R., 1159 Shannon, W. AHen, Jr., 1084 Courtright, James B., 960 Krishnapillai, V., 1159 Sharp, Phillip A., 1192 Kubinski, H., 854 Shipman, R., 1058 Dabbs, Eric R., 994 Kubinski, Z. O., 854 Shivakumar, A. G., 1205 Dempsey, Walter B., 1084 Signer, Ethan R., 1197 Denor, Patrick F., 960 La Licata, Rosaria, 833 Silhavy, Thomas J., 1070 Donoghue, Daniel J., 1192 Lark, Cynthia A., 1008 Silver, Simon, 1084 Dorian, Kenneth J., 976 Lark, Karl G., 1008 Silvius, John R., 1027 Downie, J. Allan, 844 Lawther, Robert P., 1201 Spudich, Elena Negri, 1094 Drake, H. L., 916 Legrain, C., 1187 Strominger, Jack L., 1143 Drummond, Martin H., 1178 Lo, G. C. Y., 900 Sturman, A. J., 886 Dubnau, David, 1205 Ludtke, Douglas, 1070 Dunaway-Mariano, Debra, 924 Tanenbaum, Stuart W., 874 McElhaney, Ronald N., 1027 Tilby, Michael, 1189 Fast, Robert, 839 McIntire, Sarah A., 1084 Tipper, Donald J., 1143 Feingold, B., 1143 Maeba, P. Y., 1058 Toh-e, Akio, 1002 Flashner, Michael, 874 Magill, Jane M., 924 Frasch, Carl E., 1127 Marchal, C., 1109 Ucker, David S., 1197 Marder, R., 1174 Glansdorff, N., 1187 Mergeay, M., 1187 Wang, C. Houston, 969 Goldman, Robert, 1143 Miller, Carl A., 874 Wang, Philip, 874 Gordon, Milton P., 909 Mocca, Louis F., 1127 Wanner, Barry L., 947 Green, R. S., 886 Montoya, Alice L., 909 Weiss, R., 1159 Greenblatt, J., 1109 Moore, Larry W., 909 Wickner, Reed B., 1002 Guerry, Patricia, 1002 Mora, Jaime, 880 Wilkinson, Brian J., 976 Murphy, J. R., 1135 Willetts, Neil, 1084 Hamilton, I. R., 900 Murray, R. G. E., 1037 Wurster, Bernd, 955 Hansberg, Wilhelm, 880 Hashimoto, Tadayo, 1120 Naider, F., 1174 Yoch, Duane C., 1018 Hatfield, G. Wesley, 1201 Neidhardt, Frederick C., 947 Yoshida, Nobuko, 1184 ACKNOWLEDGMENT

The following have served as invited special reviewers for the Journal during 1978, and their help is greatly appreciated.

Dinah Abram B. Wesley Catlin Jonathan Gallant Julius Adler Glenn H. Chambliss Ann Ganesan Peter Albersheim Arun K. Chatterjee Elizabeth Gantt Henry C. Aldrich Mary Dell Chilton Jeffrey F. Gardner Hussein Aleem Ian Chopra Reginald Garrett Mary M. Allen Vincent P. Cirillo Charles Geloug William Alworth Alvin J. Clark Howard Gest Giovanna Ferro-Luzzi Ames Colin H. Clarke Jean Marie Ghuysen John S. Anderson Paul S. Cohen D. T. Gibson Paul M. Anderson Sidney Cohen Frank Gibson Richard L. Anderson Murray S. Cohn Charles Gilvarg Anthony J. Andreoli Roger M. Cole Luis Glaser Andrew A. Archibald Sylvia E. Coleman Michael Glaser N. N. Aronson R. John Corier W. Goebel Alan G. Atherly M. L. Perille Collins Alfred L. Goldberg Daniel E. Atkinson Catherine Costello Howard Goldfine Frederick Ausubel N. R. Cozzarelli Terry L. Graham James Baddiley William A. Cramer J. D. Gralla E. Bakker Irving P. Crawford Joseph Greenberg Jack N. Baldwin Lolita Daneo-Moore Neil B. Groman Tom Baldwin Prasanta Datta Lorraine Gudas Clinton E. Ballou G. Daumy Sonia K. Guterman Eugene M. Barnes, Jr. Julian E. Davies Bruce Haddock Wayne R. Barnes Leodis Davis Harlyn 0. Halvorson L. S. Baron Rowland H. Davis Philip C. Hanawalt Peter Barth Benoit DeCrombrugghe R. S. Hanson Simon Baumberg R. Deering Philip E. Hartman Manfred E. Bayer Patrick P. Dennis Leland H. Hartwell Margaret Bayer Murray Deutscher Robert Haselkorn Edwin H. Beachey Roy H. Doi H. M. Hassan S. I. Beale M. I. Dolin G. W. Hatfield Robert M. Bell W. D. Donachie Robert W. Hedges Howard C. Berg W. Dowhan Maria Heincz Alan W. Bernheimer Gabriel R. Drapeau Charles E. Helmstetter Robert W. Bernlohr H. Drucker Ulf Henning Donald Berns David A. Dubnau C. Hermsdorf Dale C. Birdsell Bernard S. Dudock Ira Herskowitz Kenneth F. Bott Les Dutton M. L. Higgins Clive Bradbeer Martin Dworkin Peter Ho Philip D. Bragg Robert G. Eagon James A. Hoch Volkmar Braun C. F. Earhart Sallie 0. Hoch Roger K. Bretthauer Thomas Eckhardt D. S. Hodgins Winston J. Brill Rita Egan Chester E. Holmlund Thomas D. Brock Henry L. Ehrlich James Hopper Paul M. A. Broda Eric Eisenstadt Paul Howard-Flanders Willie Brown Frederick A. Eiserling Martha Howe Clifford Brunk Barbara Elliott F. Marion Hulett Francis L. A. Buckmire Jerald Ensign Seymour H. Hutner Kenneth Budd Harold Evans John Ingraham Robert P. Burchard Stanley Falkow J. Ingram A. Howard Bussey Robert H. Fillingame Masayori Inouye B. R. Byers Phillip Fitz-James Junetsu Ito Enrico Cabib Ernst Freese Makoto Iwaya Noel Calvin John R. Forro George A. Jacoby Joseph Calvo Audrey V. Fowler S. R. Jaskunas John H. Campbell Robert T. Fraley Roy A. Jensen Carol Caperelli Morris Friedkin Richard Jorgensen John A. Carbon James Friesen Elliot Juni Philip L. Carl Itoshi Fujita H. Ronald Kaback Lucien Caro R. C. Fuller Clarence I. Kado N. G. Carr Anthony V. Furano A. D. Kaiser Michael Cashel Michael Gabridge Eva R. Kashket Yona Kassir Dallice Mills Monica Riley Alec Keith Leonard E. Mindich Marilyn C. Roberts Norman Kelker Shoji Mizushima Howard J. Rogers K. Killick T. C. Montie W. Robert Romig Thomas G. Kinscherf K. Monty R. J. Roon Joel Kirschbaum E. C. Moore Harry Rosenberg Stephen Kistler David R. Morris John R. Roth Nancy E. Kleckner L. E. Mortenson Lawrence I. Rothfield Claude Klee Robert Mortlock W. Dean Rupp C. Kline Raymond D. Mosteller John Sadler David Knaff James W. Moulder A. St. John Arthur L. Koch David W. Mount Milton R. J. Salton G. Kohlhaw Ellen Murphy Abigail A. Salyers Paul Kolenbrander Kenneth Murray Moselio Schaechter Wil N. Konings R. G. E. Murray Sami Schaefler Jordan Konisky Teruko Nakazawa Hans U. Schairer Dennis J. Kopecko N. Nanninga Josef Schell Hans Kornberg J. B. Neilands L. Schirch M. Krichevsky David L. Nelson Karl H. Schleifer Terry A. Krulwich E. Neuhaus Jean M. Schmidt Donn J. Kushner E. Newman Carl A. Schnaitman Sidney Kushner W. A. Newton Janet L. Schottel Sanford Lacks Robert A. Niederman Maxime S. Schwartz Alan Lambowitz Donald J. Niederpruem Peter Setlow Otto Landman W. Niehaus Lucille Shapiro Arthur Landy Hiroshi Nikaido Ronald J. Sheehy J. C. Lara J. S. Nishimura Louis B. Sherman June J. Lascelles S. Nishimura Moshe Shilo E. R. Leadbetter Kurt Nordstrom Gerald D. Shockman Sylvia Lee-Huang Richard P. Novick David F. Silbert M. Leibowitz Max Oeschger Margarita Silva-Hutner Terrance Leighton Eiichi Ohtsubo Richard S. Silver Loretta Leive George Ordal Melvin Simon Margaret Lieb R. A. Ormsbee Robert D. Simoni Regina Linder L. N. Ornston Larry Simpson E. S. Lindstrom Mary Jane Osborn Michael Sinensky David Lineback Johnathan Ou Rivers Singleton, Jr. Barbara B. Lippincott Dale Oxender Ralph Slepecky William F. Loomis N. Paleroni Paul F. Smith James S. Lovett James T. Park Q. Smith Paul S. Lovett J. Parker Philip F. Sparling Donald Lueking R. Patel George Sprague, Jr. Ben J. J. Lugtenberg W. J. Payne B. G. Spratt Donald G. Lundgren J. H. Pazur Earl R. Stadtman Joan E. Lusk Harvey Penefsky Thressa C. Stadtman Werner Maas Robert D. Perry Vilma A. Stanisich J. McCord Alan Peterkofsky William Steinberg D. McCormick David E. Pettijohn S. E. Stevens Russell E. MacDonald Herman J. Phaff Roselynn Stevenson Michael R. McGinnis Paul V. Phibbs R. Stjernholm Carolyn MacGregor Olga Pierucci B. A. D. Stocker James D. Macmillan B. Plapp Kenneth P. Strickland Joan Macy G. W. E. Plaut Isamu Suzuki Boris Magasanik Jeanne S. Poindexter H. E. Swaisgood R. Maier John Postgate Paul S. Sypherd G. R. Mandell Anthony Pugsley F. Robert Tabita Jack Maniloff William K. Purves Celia Tabor A. G. Marr C. Raetz Herbert Tabor J. Joseph Marr Arnold W. Ravin F. P. Tally Barry Marrs Dan S. Ray Austin L. Taylor Warren E. Masker Shmuel Razin Bonnie Templeton Tom Mason Elvin T. Reese Ethel Tessman C. R. Matthews Peter R. Reeves C. B. Thorne Ann Matthysse Bernard E. Reilly Donald J. Tipper E. Miles Albey M. Reiner Hajime Tokuda C. Miller D. W. Ribbons N. E. Tolbert James R. Miller Mark H. Richmond Alexander Tomasz Robert V. Miller Howard V. Rickenberg Jun-ichi Tomazawa Penelope Toothman Richard L. Weiss C. Peter Wolk H. Edwin Umbarger E. W. Westhead Paul P. K. Y. Wong Raymond C. Valentine Maryamne H. Whatley Barbara E. Wright J. D. A. Van Embden M. Wheelis Henry C. Wu Joseph E. Varner Thomas J. White Charles Yanofsky James Vary Frank Wightman D. Yoch Barbara Vold Gary Wilcox George Yokum Graham C. Walker Neil S. Willetts E. Younathan Judy D. Wall Luther S. Williams Frank E. Young D. E. Wampler Peter A. Williams Ian Young Barry Wanner Gary A. Wilson S. Zakrzewski David Waxman H. Winter Patrice J. Zamenhof Robert B. Webb R. S. Wolfe J. G. Zeikus Bernard Weisblum Lloyd Wolfinbarger David Zipser Emilio Weiss 1979 Full Membership- American Society for Microbiology 1913 I St., NW, Washington, DC 20006

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A joint effort by members ofthe American Societyfor Microbiologyand the American Association of Immunologists This definitive and much needed new book is a companion volume to the highly acclaimed Manual of Clinical Microbiology, 2nd Edition. It has been written to provide a current, comprehensive, and definitive source of information about the best methods for conducting specific immunological tests in laboratory immunology. It deals exclusively with the applications of immunology to the detection and analysis of a wide variety of diseases, including, but not limited to, those induced by microorganisms. The clinical interpretations of each procedure are detailed, and pitfalls and problems in performing the various analyses are discussed. The contents, by section, are: Tests for humoral components of the immunological response - Tests for cellular components of the immunological response - Immunoassays - Bacterial, mycotic, and parasitic immunology - Viral, rickettsial, and chlamydial immunology - Immunohematology - Laboratory examination of patients with allergic and immunodeficiency diseases - Autoimmune diseases - Tumor immunology - Transplantation immunology -Licensure and certification programs in clinical immunology laboratories. Publication date: 16 August 1976. 932 pages. Available in two bindings: Cloth, at $20.00 (ISBN: 0-914826-09-3). Flexible, at $16.00 (ISBN: 0-914826-09-3). (Note: Members ofA SM ma, purchase this book at the special member discount price of$12.00 cloth, $10.00 flexible. Member purchases arefor personal use onlv'. Pa vment must accompany member orders.) Publishedand AMFRICAN SOCIETY FOR MICROBIOLOGY distributed by: 1913 I Street, N.W., Washington, D.C. 20006

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1. 1979 Dues (includes ASM News)...... $2.50 2. Additional joumals may be subscribed to at the following rates. Please enter the higher rate (column B) if you reside outside the U.S. and its possessions. A B Journal of Bacteriology (JB)...... 1. $22 $30 Antimicrobial Agents and Chemotherapy (AAC)...... 2. $16 $19 Applied and Environmental Microbiology (AEM)...... 3. $17 $22 International Journal of Systematic Bacteriology (IJSB)...... 4. $15 $15 Journal of Virology (JVI)...... 5. $21 $26 Journal of Clinical Microbiology (JCM)...... 6. $16 $19 Microbiological Reviews (MR)...... 7. $9 $10 Infection and Immunity (IAI)...... 9. $21 $26 3. TOTAL and Remit...... S

RATES ARE FOR 1979 ONLY Please enclose payment with application. AUTHOR INDEX VOLUME 136

Agabian, Nina, 795 Dabbs, Eric R., 994 Gorenstein, Charles, 142 Aguilar, Juan, 522 Dagley, Stanley, 449 Grant, Robert B., 808 Akagi, J. M., 916 Daneo-Moore, L., 491 Green, R. S., 886 Ames, Giovanna Ferro-Luzzi, Daniels, L., 75 Greenblatt, J., 1109 1094 Davis-Mancini, Kathleen, 625 Guerry, Patricia, 1002 Anderson, A. J., 886 DeMartini, Marc, 795 Guymon, Lawrence F., 391 Anderson, James J., 168 Dempsey, Walter B., 1084 Anderson, Jeffrey J., 449 Dendinger, Susan M., 588 Hachisuka, Yoetsu, 484 Anraku, Yasuhiro, 5 Deneke, Carol F., 148 Hacking, Andrew J., 522 Aono, Rikizo, 812 Denor, Patrick F., 960 Hageman, James H., 625 Archibald, A. R., 886 De Scrilli, A., 55 Hahn, Jeanette, E., 117 Archibald, Fred S., 35 DeVoe, I. W., 35 Hall, Prudence J., 786 Argast, Manfred, 1070 Diekert, Gabriele B., 597 Hamilton, I. R., 900 Arnon, Daniel I., 1018 DiRienzo, Joseph M., 148, 158 Hammer-Jespersen, Karin, 668 Arthur, Helen, 815 Dobrzaniski, W. T., 295 Hancock, Robert E. W., 381 Atkinson, Katharine D., 142 Doddema, Hans J., 19 Hansberg, Wilhelm, 880 Ayling, Peter D., 588 Donoghue, Daniel J., 1192 Hansen, Tonny Dedenroth, 668 Dorian, Kenneth J., 976 Harada, Tokuya, 714 Baca, 0. G., 429 Dougan, Gordon, 742 Hashimoto, Tadayo, 1120 Bacha, P., 1135 Douthit, H. A., 209 Hatfield, G. Wesley, 1201 Bailone, Adriana, 441 Downie, J. Allan, 844 Haury, John F., 688 Baldwin, J. N., 402 Drake, H. L., 916 Heath, H. E., 638 Becker, J. M., 1174 Drapeau, Gabriel R., 607 Hederstedt, Lars, 304 Berg, Douglas E., 419 Dreyfus, Lawrence A., 757 Hedges, R. W., 1159 Bhattacharjee, J. K., 647 Drummond, Martin, 419, 1178 Heffron, Fred, 742 Biek, Donald P., 1094 Dubnau, David, 1205 Heincz, Maria C., 96, 104 Biraghi, A., 55 Dunaway-Mariano, Debra, 924 Hennen, Paula E., 929 Blumenthal, Harold J., 1120 Henning, Ulf, 280, 1189 Boonstra, Johannes, 844 Elliott, W. H., 24 Hespell, Robert B., 936 Boos, Winfried, 1070 Higashiura, Tadashi, 714 Boyen, A., 1187 Faelen, M., 477 Hindennach, Ingrid, 1189 Braverman, S., 491 Falke, Ernest V., 142 Hinks, E. Ternove, 491 Brenchley, Jean E., 588 Falkow, Stanley, 742 Hirochika, Hirohiko, 983 Brennan, Edward F., 790 Fast, Robert, 839 Hirota, Yukinori, 280 Brill, Winston, J., 247, 253, 267 Feingold, B., 1143 Hofnung, M., 1109 Brown, Gene M., 657 Felix, A., 352 Holloway, B. W., 507 Brubaker, Robert R., 757, 786 Fillingame, Robert H., 570 Holmgren, Erik, 304 Bukhari, A. I., 423 Finnerty, W. R., 803 Hoober, J. Kenneth, 359 Burrows, Robert B., 657 Flashner, Michael, 874 Horwitz, A. H., 565 Buxton, R. S., 668 Foulds, John, 501 Hou, Ching T., 352 Fox, Maurice S., 455 Hutten, Theo J., 19 Calhoun, David H., 117 Foy, James J., 647 Camargo, E. Plessmann, 1184 Francesconi, Margaret Day, 614 Ichikawa, Tsutomu, 63 Campomanes, Miguel, 880 Frasch, Carl E., 1127 Iwaya, Makoto, 1143 Carbone, M. L., 55 Fredricks, Walter W., 219 Carithers, Robert P., 822, 1018 Fuchs, P. G., 295 Jacob, A. E., 1159 Carrano, Carl J., 69 Fulbright, D. W., 497 Jacoby, G. A., 1159 Carter, Harry B., 929 Funanage, Vicky L., 588 Jannasch, Holger W., 765 Casida, L. E., Jr., 565 Furukawa, Hidehiko, 693 Jinks, David C., 1027 Catlin, B. Wesley, 131 Johansson, Karl-Erik, 324 Chai, Tuu-jyi, 501 Gaudy, Elizabeth T., 638 Johnson, Robert C., 125 Chilton, Mary-Dell, 1178 Gayda, Randall C., 369 Johnston, Gerald C., 318 Cirillo, V. P., 49 Gholson, R. K., 136 Cole, Ronald S., 538 Gill, Ronald, 742 Kadner, Robert J., 1050 Cooper, Terrance G., 234 Ginsberg, Dalia, 111 Kang, Shyue yue, 867 Coulton, J. W., 1037 Glansdorff, N., 1187 Keck, W., 723 Courtright, James B., 960 Goldman, Robert, 1143 Kelley, Bruce C., 201 Currier, Thomas C., 682 Gordon, Milton P., 1143 Kennedy, Eugene P., 85 ii AUTHOR INDEX J. BACTERIOL. Keynan, Alex, 111 Morrison, D. A., 548 Schottel, Janet, 1084 Khatoon, H., 4232 Morrison, John F., 1 Schwartz, Maxime, 700 Kieras, R. M., 209 Morton, Heather, 815 Schwarz, Heinz, 280 Kinscherf, Thomas G., 1084 Motojima, Kiyoto, 5 Schwarz, U., 723 Klapwijk, Pieter M., 775 Murooka, Yoshikatsu, 714 Serna, Leticia, 341 Knoebel, Kathy, 570 Murphy, J. R., 1135 Setlow, Barbara, 433 Kobayashi, Yasuo, 983 Murray, R. G. E., 1037 Setlow, Peter, 331, 433 Koch, Arthur L., 969 Mutoh, Norihiro, 693 Shannon, W. Allen, Jr., 1084 Kodaira, Ryoji, 947 Meyers, W. F., 452 Shapely, Aina J., 808 Konings, Wil N., 844 Sharp, Phillip A., 1192 Korfhagen, T. R., 1159 Nagahari, Kenji, 312 Shinners, Elizabeth Nash, 131 Krishnapillai, V., 1159 Naider, F., 1174 Shipman, R., 1058 Kubinski, H., 854 Namiki-Kanie, Sachiyo, 484 Shivakumar, A. G., 1205 Kubinski, Z. O., 854 Nanninga, N., 723 Signer, Ethan R., 1197 Kubitschek, H. E., 179 Navre, J. M., 631 Silhavy, Thomas J., 1070 Neidhardt, Frederick C., 947 Silver, Simon, 1084 Lagenaur, Carl, 795 Nester, Eugene W., 909 Silvius, John R., 1027 La Licata, Rosaria, 833 Newman, C. N., 179 Simon, Robert D., 414 Langley, Keith E., 85 Nikaido, Hiroshi, 227, 381 Sinden, Richard R., 538 Lark, Cynthia A., 1008 Nomura, Shintaro, 818 Sklar, Robert, 460 Lark, Karl G., 1008 Nunn, William D., 929 Smith, Oliver H., 219 Lawther, Robert P., 1201 Smith, P. F., 829 Leary, J. V., 497 O'Connor, R., 24 Sonntag, Ingeborg, 280 Legrain, C., 1187 Odelson, D. A., 936 Sparling, P. Frederick, 391 Leibowitz, Paul J., 455 O'Donovan, Gerard A., 825 Sparnins, Velta L., 449 Levine, Alain, 441 Okubo, Yasuhito, 63 Spudich, Elena Negri, 1094 Lew, Henry C., 227 Omans, Judson, 449 Stadler, David, 341 Lin, E. C. C., 522 Oxender, D. L., 168 Strominger, Jack L., 1143 Lo, G. C. Y., 900 Sturman, A. J., 886 Loeb, A., 631 Palacios, Rafael, 880 Sumrada, Roberta, 234 Lommen, Mary Ann Jordan, 730 Palva, E. T., 286 Supiano, Mark A., 253 Lopez, Ismael P., 625 Pan, Pauline, 955 Suzuki, Shigeo, 63 Lucchini, G., 55 Parks, L. W., 531 Ludtke, Douglas, 1070 Patel, K. R., 829 Takemoto, Jon, 730 Patel, Ramesh, N., 352 Tamura, Gakuzo, 812, 818 McElhaney, Ronald N., 1027 Pauley, Robert J., 219 Tanenbaum, Stuart W., 874 McFall, Elizabeth, 96, 104 Peak, M. J., 437 Taylor, F. R., 531 McGrath, B. E., 631 Pierucci, O., 631 Thauer, Rudolf K., 597 McIntire, Sarah A., 1084 Pollack, Jordan H., 1120 Thompson, John, 465 MacLeod, Robert A., 148, 158 Pope, William T., 191 Tilby, Michael J., 10, 1189 MacNeil, Douglas, 247, 253, 267 Postle, Kathleen, 1165 Tipper, Donald J., 1143 MacNeil, Tanya, 253, 267 Powell, Janet T., 1 Toh-e, Akio, 1002 Maeba, P. Y., 1058 Preston, R. A., 209 Torregrossa, R. E., 803 Magill, Jane M., 924 Toussaint, A., 477 Magni, G. E., 55 Quinto, Carmen, 880 Toyoda, Hiroo, 799 Miikela, P. Helena, 227 Tsai, Wen-cherng, 582 Makula, R. A., 803 Raymond, Kenneth N., 69 Tyrrell, Rex M., 437 Marchal, C., 1109 Reeves, Robert H., 191 Marder, R., 1174 Reichenbach, Hans, 708 Ucker, David S., 1197 Markovitz, Alvin, 369 Reznikoff, William S., 1165 Ulaszewski, S., 49 Maruo, Bunji, 818 Riazi, Jail, 1008 May, B. K., 24 Roberts, Gary P., 253, 267 van der Drift, Chris, 19 Mayberry, W. R., 829 Rocha, Victor, 790 Verwer, R. W. H., 723 Mayer, Hubert, 708 Romano, Nino, 833 Vignais, P. M., 201 Mergeay, M., 1187 Rose, B., 1174 Vogels, Godfried D., 19 Meyer, Jacques, 201 Rothman, Robert H., 444 Miller, Carl A., 874 Rutberg, Blanka, 304 Walstad, Diana L., 391 Miller, Elizabeth T., 175 Rutberg, Lars, 304 Wang, C. Houston, 969 Miyata, Hisao, 558 Wang, Philip, 874 Miyata, Machiko, 558 Sabath, L. D., 976 Wanner, Barry L., 947 Mizushima, Shoji, 693 Sakakibara, Shigeki, 136 Warfel, L. E., 452 Mocca, Louis F., 1127 Sanchez, Federico, 880 Watson, J. M., 507 Mock, Michele, 700 Sasaki, Takashi, 818 Watson, Kenneth, 815 Montoya, Alice L., 909 Schafer, Dorothy, 874 Weeks, Owen B., 614 Moore, Larry W., 909 Scheulderman, Trudy, 775 Weiss, R., 1159 Mora, Jaime, 880 Schilperoort, Robert A., 775 Westby, Carl A., 582 iii VOL. 136, 1978 AUTHOR INDEX Whiteley, Mary H., 808 Wisseman, C. L., Jr., 452 Yamane, Kunio, 818 Wickner, Reed B., 1002 Wolk, C. Peter, 682, 688 Yamasaki, Makari, 812, 818 Wicks, Frank D., 136 Womack, John E., 825 Yamato, Ichiro, 5 Wilkinson, Brian J., 976 Woodward, J. R., 49 Yasuda-Yasaki, Yoko, 484 Willetts, Neil, 1084 Wopat, Ann E., 570 Yoch, Duane C., 822, 1018 Willison, J. H. Martin, 318 Wr6blewski, Henri, 324 Yoshida, Nobuko, 1184 Wilson, C. R., 402 Wurster, Bernd, 955 Yoshikawa, Kirhoshi, 799 Winkler, Herbert H., 175 Wirsen, Carl O., 765 Yamaguchi, Kazuo, 799 Zeikus, J. G., 75

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i L Y-.. i 2 1I . 097 * SUBJECT INDEX VOLUME 136

Acholeplasma laidlawii N. gonorrhoeae, 131 ATPase activity, membrane-bound, 1027 ornithine formation, 131 membrane proteins, crossed immunoelectrophoresis Arginine genes of, 324 E. coli, from, 1187 Acid phosphatase P. fluorescens, expression in, 1187 S. pombe cell cycle growth, 558 Aromatic amino acid pathway, mutations in Acinetobacter sp. HO1-N S. cerevisiae sporulation, 55 phosphatidylethanolamine hydrolysis, 803 Aromatic amino acid transferase phosphatidylglycerol hydrolysis, 803 E. coli leucine biosynthesis, 1 phospholipase A, 803 Arthrobacter Actinomycin D-sensitive mutant polypeptide, photodynamically induced cell-surface, S. cerevisiae, 142 359 Active transport Arthrobacter sialophilus energy supply, 844 neuraminidase synthesis, 874 E. coli grown anaerobically, 844 Arthrosporulation Adenosine triphosphatase activity, membrane-bound carotenogenesis, 1120 A. laidlawii, 1027 T. mentagrophytes, 1120 Adenylate cyclase- and cyclic AMP receptor protein- Arylsulfatase gene (atsA) deficient mutants K. aerogenes, 714 E. coli, 812 regulation in hybrids, 714 Agrobacterium Aspartase deficiency octopine-degrading enzymes, genes coding for, 909 Y. pestis, 757 Ti plasmids, 1178 ATP hydrolysis and synthesis Agrobacterium tumefaciens M. thermoautotrophicum, 19 octopine degradation, 775 ATP synthetase complex Ti plasmids, transfer of, 775 M. thermoautotrophicum, 19 Agromyces ramosus atsA L-forms, reversion of, 565 K. aerogenes, 714 Alkylation regulation in hybrids, 714 B. subtilis transforming DNA, 854 att4i80-tonB-trp region Alteromonas haloplanktis E. coli, 1165 lipopolysaccharide in cell wall, 148, 158 Autolytic enzyme system Amino acid aminotransferase S. faecalis, 491 E. coli leucine biosynthesis, 1 temperature effects, 491 Amino acid pathway, mutations in Azotobacter vinelandii S. cerevisiae sporulation, 55 ferredoxin I, high- and low-potential 4Fe-4S* cen- Amino acid transport ters, 822 E. coli, 168 genetic separation of high- and low-affinity systems, Bacillus amyloliquefaciens 168 extracellular protease, 24 Amino acid uptake mRNA pool, modulation of, 24 S. typhimurium mutants, 588 Bacillus cereus Ammonotelism ribosomal subunits from spores, 209 Blastocrithidia, 1184 Bacillus megaterium Crithidia, 1184 cGMP in spores and cells, 433 Herpetomonas, 1184 proteins degraded during germination, 331 Leishmania, 1184 spore germination, proteins degraded during, 331 Leptomonas, 1184 Bacillus subtilis Trypanosoma, 1184 alkylation of transforming DNA, 854 Anabaena variabilis benzeneboronic acid, 625 uridylic acid biosynthesis, 682 cell wall assembly, 886 Anabaena variabilis mutants chromosome-membrane association, 799 nitrogenase activity, oxygen-sensitive, 688 crossed immunoelectrophoresis, 304 Aquaspirillum serpens detergent-resistant variants, 10 flagella-envelope associations, 1037 DNA synthesis and spore outgrowth, 111 Arginine biosynthesis 1,3-propane sultone, 854 citrulline formation, 131 ,f-propiolactone, 854 iv VOL. 136, 1978 SUBJECT INDEX V RNA polymerase mutations, 983 flagellar hooks, 795 spore germination, inhibition of, 484 Cell cycle arrest signal spore outgrowth and DNA synthesis, 111 protein degradation, 234 sporulation, inhibition by benzeneboronic acid, 625 S. cerevisiae, 234 sporulation, temperature sensitive, suppression of, vacuole permeability, 234 983 Cell envelope succinic dehydrogenase mutants, 304 N. gonorrhoeae, antibiotic-sensitive and -resistant tunicamycin resistance, 818 strains of, 391 Bacteriophage Cell fractions B. subtilis cell wall, 886 U. urealyticum, 833 Bacteriophage IKe Cellular slime molds E. coli, 808 folic acid inactivation, 955 incompatibility group P plasmids, 808 Cellulomonas dehydrogenans propagation, 808 squalene, 614 Bacteriophage lambda sterols, 614 insertion into E. coli deo operon, 668 Cell wall assembly mercury resistance genes, 1084 B. subtilis, 886 plasmid R100 DNA, 1084 Cell wall composition Bacteriophage Xdeo+, 668 methicillin-resistant S. aureus, 976 Bacteriophage XcollOO, 1192 Cell wall material, incorporation Bacteriophage Mu B. subtilis, 886 DNA modification depends on a host function, 423 concanavalin A, 886 E. coli, 423 phage, 886 Bacteriophage receptor phosphate limitation, 886 E. coli K-12, 693 turnover, 886 lipopolysaccharide, 693 Centrifugation-electrophoresis procedure for purifying outer membrane protein, 693 chromosome-membrane complexes T4, 693 B. subtilis, 799 Bakers' yeast mannan, immunochemical properties of Chloramphenicol phosphate content, 63 E. coli, 460 Bdellovibrio bacteriovorus nitrosoguanidine mutagenesis, enhancement of, 460 growth on E. coli, 936 Chromatophores RNA-ribose metabolism, 936 R. sphaeroides, 730 Benzeneboronic acid Chromosomal DNA B. subtilis sporulation, 625 B. subtilis, 1205 Bisulfite reduction, dissimilatory hydroxyurea, 1205 D. vulgaris, 916 Chromosome-membrane association Blastocrithidia B. subtilis, 799 ammonotelism, 1184 centrifugation-electrophoresis procedure used for ureotelism, 1184 purification, 799 Branched-chain amino acid transport systems Chromosome replication E. coli, 168 E. coli B/r strains, 179 genetic separation of high- and low-affinity systems, Citrulline formation 168 N. gonorrhoeae, 131 B12 receptor Cloned DNA fragment specifying major outer mem- E. coli, 1050 brane protein a synthesis, repression of, 1050 E. coli, 369 Cloning vehicles, molecular Calcium S. aureus, 402 A. ramosus L-forms, reversion of, 565 Clostridium formicoaceticum Candida slooffii CO oxidation, 597 thermal adaptation, 815 Clostridium thermoaceticum Carbon monoxide oxidation CO oxidation, 597 C. formicoaceticum, 597 Colicin E3 C. thermoaceticum, 597 bypass of receptor-mediated resistance, 1189 Carotenogenesis E. coli, 1189 T. mentagrophytes arthrosporulation, 1120 Colicin E3 production Catabolite repression E. coli harboring plasmids, 700 E. coli lac operon expression, 947 Complementation Catabolite-repression-like phenomenon Tn3 mutants, 742 R. meliloti, 1197 Concanavalin A Catalase action B. subtilis cell wall, 886 R. prowazeki, 452 Conidia Caulobacter crescentus N. crassa, 341 vi SUBJECT INDEX J. BACTERIOL. nuclear division, 341 phage Mu, 423 Corn stunt spiroplasma DNA replication lipids, 829 dnaT, 1008 Corynebacterium diphtheriae E. coli, 1008 extragenic suppressor strains, 1135 DNA synthesis Coxiella burnetii B. subtilis spore outgrowth, 111 ribosomes, 429 dnaT Crithidia E. coli DNA replication, 1008 ammonotelism, 1184 DNA, transforming ureotelism, 1184 alkylation, 854 Crossed immunoelectrophoresis B. subtilis, 854 A. laidlawii membrane proteins, 324 dsdC activator B. subtilis succinic dehydrogenase mutants, 304 E. coli, 96 sodium deoxycholate, 324 D-serine deaminase synthesis, 96 Tween 20, 324 CTP:phosphatidic acid cytidylyltransferase Eclipse complex protein E. coli membranes, 85 pneumococcus transformation, 548 Cyanobacteria Energy supply DNA, extrachromosomal, 414 E. coli anaerobic transport, 844 6-Cyanopurine Enterobacter aerogenes indicator used for isolating nifmutations in K. pneu- tryptophan synthase #2 protein, 790 moniae, 247 Envelope-flagella associations Cyclic AMP and cyclic AMP receptor protein A. serpens, 1037 E. coli, 812 Envelope proteins Cyclic GMP cyclic AMP and cyclic AMP receptor protein, 812 B. megaterium cells and spores, 433 E. coli mutants, 812 Cytochrome c Enzymatic activity purple photosynthetic bacteria, 1018 U. urealyticum, 833 Enzyme activity Deaminase deletion plasmids, 312 E. coli riboflavin biosynthesis, 657 Erwinia carotovora Deletion plasmids tryptophan synthase #2 protein, 790 transformants of P. aeruginosa trp cells with Escherichia coli RSF1010-trp hybrid plasmid, 312 active transport, anaerobic, 844 Deletions amino acid aminotransferase, 1 E. coli amino acid transport systems, 168 Mu-i induced, 477 anaerobic transport, 844 deo operon arginine genes expressed in P. fluorescens, 1187 E. coli, 668 atsA of K. aerogenes, 714 Xdeo+ transducing phages, 668 attO80-tonB-trp region, 1165 Desulfovibrio vulgaris B. bacteriovorus grown on, 936 bisulfite reduction, dissimilatory, 916 branched-chain amino acid transport systems, 168 Detergentrresistant variants B12 receptor, 1050 B. subtilis, 10 catabolite repression, 947 "Diaminopimelic acid spheroplasts" cell division during shift-up, 631 E. coli, 441 cell envelope and shape, 280 lysogenic induction, lack of, 441 chloramphenicol enhances nitrosoguanidine muta- Dictyostelium discoideum genesis, 460 folic acid inactivation, 955 chromosome replication in B/r strains, 179 Dimer excision colicin E3, bypass of receptor-mediated resistance E. coli recL152 mutant, 444 to, 1189 DNA, cross-linked colicin E3 production, 700 E. coli treated with psoralen and light, 538 CTP: phosphatidic acid cytidylyltransferase, 85 repair, 538 cyclic AMP and cyclic AMP receptor protein, 812 DNA, extrachromosomal deletions, Mu-induced, 477 cyanobacteria, filamentous, 414 deo operon, 668 DNA homology dimer excision in recL152 mutant, 444 Agrobacterium Ti plasmids, 1178 DNA (cross-linked), repair of, 538 DNA-membrane association DNA metabolism, effects of mutations influencing, B. subtilis, 799 538 centrifugation-electrophoresis procedure used for DNA replication, mutation affecting, 1008 purification, 799 DNA sequences homologous to Tn5 (Kan), absence DNA modification of, 419 E. coli, 423 dnaT, 1008 host function, 423 dsdC activator, 96 VOL. 136, 1978 SUBJECT INDEX vii energy coupling for D-lactate, 867 tryptophan synthase f2 protein, 790 energy supply for anaerobic transport, 844 tynA of K. aerogenes, 714 envelope proteins, 812 uracil uptake mutants, 839 F plasmid cosegregation, 455 vitamin B12 receptor, 1050 genetic separation of high- and low-affinity amino Extrachromosomal DNA acid transport systems, 168 cyanobacteria, filamentous, 414 glycan chains in the sacculus, 723 Extragenic suppressor strains glycerol-induced growth stasis, 929 C. diphtheriae, 1135 sn-glycerol-3-phosphate transport, 1070 growth on simple and complex media, 969 Ferredoxin I histidine operon expression, 1120 A. vinelandii, 822 incompatibility group P plasmids, 808 high- and low-potential 4Fe-4S* centers, 822 indole-3-propionic acid, 219 Flagella-envelope association isoleucine-valine biosynthesis, suppressors of muta- A. serpens, 1037 tion affecting, 117 Flagellar hooks kasugamycin-dependent mutants, 994 C. crescentus, 795 lac operon expression, 947 Folic acid inactivation D-lactate transport, 867 D. discoideum, 955 Xdeo+ transducing phages, 668 P. violaceum, 955 leucine biosynthesis, 1 F plasmid cosegregation light treated, 538 E. coli chromosome, DNA strands of, 455 lipopolysaccharide and T4 receptor activity, 693 Fructose-1,6-bisphosphatase lysogenic induction in "diaminopimelic acid sphe- S. cerevisiae, 647 roplasts," 441 macromolecular synthesis during glycerol-induced f)-Galactosidase and lactose-P-enolpyruvate phospho- growth stasis, 929 transferase system, co-induction of malB region, 1109 S. mutans, 900 morphological mutant, 1143 S. salivarius, 900 nitrosoguanidine mutagenesis, enhancement of, 460 Gel electrophoresis nmpA, location of, 501 ribosomes from C. burnetii and E. coli, 429 nucleoside triphosphate levels during glycerol-in- Gliding bacteria duced growth stasis, 929 restriction endonucleases, 708 nutritional shift-up, 631 Glutamine synthetase orotic acid excretion, 825 S. typhimurium, 588 outer membrane protein, 501 Glutamine synthetase mRNA levels outer member protein a, 369 nitrogen source regulation, 880 outer membrane protein and T4 receptor activity, N. crassa, 880 693 Glycan chain arrangement outer membrane proteins, mutants missing, 280 E. coli sacculus, 723 phage IKe propagation, 808 Glycerol catabolism phage Mu-induced deletions, 477 hexosephosphate derivatives, metabolism, 638 phage Mu-induced modification of DNA, 423 P. aeruginosa, 638 phage T4 receptor activity, 693 Glycerol-induced growth stasis polymerase III activity, 125 E. coli, 929 postreplication DNA repair, 125 Glycerol-3-phosphate dehydrogenase-defective mu- proline transport carrier-defective mutants, 5 tants propanediol pathway, 522 N. crassa, 960 proton-translocase mutants, isolation of, 570 sn-Glycerol-3-phosphate transport psoralen treated, 538 E. coli, 1070 quinolinate biosynthesis, 136 Glycolysis receptor-mediated resistance to colicin E3, bypass S. lactis, 465 of, 1189 Growth recL152 mutant, 444 E. coli on simple and complex media, 969 recombination, effects of mutations influencing, 538 Growth stasis, glycerol-induced repair replication patch size in recL152 mutant, 444 E. coli, 929 rho gene product, 1201 riboflavin biosynthesis, 657 Herpetomonas ribosomes, 429 ammonotelism, 1184 sacculus, 723 Hexosephosphate derivatives, metabolism of D-serine deaminase synthesis, 96, 104 glycerol catabolism, 638 shift-up, nutritional, 631 P. aeruginosa, 638 tonB region, 1165 Histidine operon Tn5 (Kan), absence of DNA sequences homologous E. coli, 1201 to, 419 rho gene product, 1201 tryptophan operon derepression, 219 Histidine transport genes Viii SUBJECT INDEX J. BACTERIOL. mutant selection, use for, 1094 thermal adaptation, 815 S. typhimurium, 1094 L-forms Hydrogen peroxide production A. ramosus, 565 R. prowazeki, 452 reversion, effects of magnesium, calcium, and serum 4-Hydroxyphenylacetic acid degradation on, 565 T. cutaneum, 449 Light Hydroxyurea R. capsulata nitrogenase, 201 B. subtilis plasmid and chromosomal DNA, 1205 Light-treatment E. coli, 538 Immunological techniques Linkage map ribosomes from C. burnetii and E. coli, 429 P. aeruginosa PAT, 507 Inactivation of bacteria Lipids UV radiations of different energies, 437 corn stunt spiroplasma, 829 Incompatibility group P plasmids S. citri, 829 E. coli, 808 Lipopolysaccharide phage IKe propagation, 808 E. coli, 693 Indole-3-propionic acid marine bacterium, 148, 158 E. coli, 219 phage T4 receptor activity, 693 tryptophan operon, derepression of, 219 Lysis, temperAture effects on Induction, lysogenic S. faecalis, 491 "diaminopimelic acid spheroplasts," 441 Lysogenic induction, lack of E. coli, 441 "diaminopimelic acid spheroplasts," 441 Inositol-less death E. coli, 441 S. cerevisiae membrane damage, 49 Iron Macromolecular synthesis N. meningitidis, 35 E. coli glycerol-induced growth stasis, 929 Iron uptake and rhodotorulic acid Magnesium R. pilimanae, 69 A. ramosus L-forms, reversion of, 565 Isoleucine-valine biosynthesis malB region E. coli, 117 E. coli, 1109 suppressors of mutation affecting, 117 restriction map, 1109 specialized transducing phages, 1109 Kasugamycin-dependent mutants Maltose E. coli, 994 S. typhimurium outer membrane protein, 286 Klebsiella aerogenes Marine bacterium atsA, 714 lipopolysaccharide, 148, 158 tynA, 714 Membrane-chromosome association Klebsiellapneumoniae B. subtilis, 799 nifgenes, 247, 253, 267 Membrane damage S. cerevisiae inositol-less death, 49 lac operon expression Membrane lipid modulation catabolite repression, 947 A. laidlawii, 1027 E. coli, 947 ATPase activity, 1027 D-Lactate Membrane vesicles, spheroplast-derived autoenergized transport, 867 R. sphaeroides, 730 energy coupling, 867 Mercury resistance genes E. coli, 867 phage lambda carrying plasmid R100 DNA, 1084 Lactose-P-enolpyruvate phosphotransferase system Metalloprotease and,B-galactosidase, co-induction of staphylococcal protease, 607 S. mutans, 900 Methane oxidation, 352 S. salivarius, 900 Methanobacterium ruminantium XcollOO, 1192 one-carbon metabolism, 75 Leishmania Methanobacterium thermoautotrophicum ammonotelism, 1184 ATP hydrolysis and synthesis, 19 ureotelism, 1184 one carbon metabolism, 75 Leptomonas Methanol oxidation of, 352 ammonotelism, 1184 Methanosarcina barkeri ureotelism, 1184 one-carbon metabolism, 75 Leucine biosynthesis Methicilhin-resistant Staphylococcus aureus E. coli amino acid aminotransferase, 1 cell walls, 976 Leucosporidium frigidum Molecular cloning vehicles thermal adaptation, 815 S. aureus, 402 Leucosporidium gelidum mRNA pool, modulation of thermal adaptation, 815 B. amyloliquefaciens extracellular protease, 24 Leucosporidium nivalis Mutant selection VOI,. 136, 1978 SUBJECT INDEX ix

S. typhimurium histidine transport genes, 1094 N. gonorrhoeae, 131 Myxococcus xanthus Orotic acid excretion myxospore formation, 1058 E. coli, 825 32Pi incorporation, 1058 Outer membrane purine anabolism and cell change, 582 P. aeruginosa, 381 permeability barrier, 381 Neisseria gonorrhoeae Outer membrane protein(s) arginine biosynthesis, 131 a, 369 cell envelope alterations in antibiotic-sensitive and DNA fragment (cloned) specifying, 369 -resistant strains, 391 E. coli, 369, 693 citrulline formation, 131 E. coli mutants, 280 ornithine formation, 131 E. coli nmpA locus, 501 Neisseria meningitidis maltose induction, 286 iron, 35 N. meningitidis, 1127 Neuraminidase synthesis phage T4 receptors activity, 693 A. sialophilus, 874 S. typhimurium, 286 Neurospora crassa glutamine synthetase mRNA levels, 880 Peptide transport deficient mutant glycerol-3-phosphate dehydrogenase-defective mu- S. cerevisiae, 1174 tants, 947 Pesticin nuclear division in germinating conidia, 341 spheroplast-like structures, generation of, 786 nucleoside transport, 924 Yersinia, 786 nif genes Phage-plasmid hybrid vector, 1192 6-cyanopurine used for isolating mutations, 247 Phosphate content K. pneumoniae, 247, 253, 267 baker's yeast mannan, immunochemical properties mapping and complementation analysis, 253 of, 63 protein products, 267 Phosphate limitation Nitrogenase B. subtilius cell wall, 886 light, effects of, 201 Phosphatidylethanolamine hydrolysis R. capsulata, 201 Acinetobacter sp. HO1-N Nitrogenase activity, oxygen-sensitive phospholipase A, 803 A. variabilis mutants, 688 Phosphatidylglycerol hydrolysis Nitrogen source Acinetobacter sp. HO1-N glutamine synthetase mRNA levels in N. crassa, phospholipase A, 803 880 Phosphofructokinase Nitrosoguanidine mutagenesis S. cerevisiae, 647 chloramphenicol, enhancement by, 460 Phospholipase A E. coli, 460 Acinetobacter sp. HO1-N, 803 nmpA phosphatidylethanolamine hydrolysis, 803 E. coli outer membrane protein, 501 phosphatidylglycerol hydrolysis, 803 Nuclear division cycle Phospholipid composition N. crassa germinating conidia, 341 R. prowazeki, 175 Nuclear pore diameters Phosphorylated compounds, acid-soluble S. cerevisiae Gl-arrested cells, 318 M. xanthus myxospore formation, 1058 Nucleoside transport Plasmid DNA N. crassa, 924 B. subtilis, 900 Nucleoside triphosphate levels hydroxyurea, 900 E. coli glycerol-induced growth stasis, 929 Plasmid, incompatibility group P Nucleotides E. coli, 808 M. xanthus, 1058 phage IKe propagation, 808 32Pi incorporation, 1058 Plasmid R68 Nutritional shift-up chromosome donor ability, 497 E. coli cell division, 631 P. glycinea, 497 Plasmid R68.45 Octopine-degrading enzymes chromosome donor ability, 497 Agrobacterium, 909 P. glycinea, 497 multiple genes coding for, 909 Plasmid R68.185 Octopine degradation chromosome donor ability, 497 A. tumefaciens, 775 P. glycinea, 497 Ti plasmids, transfer of, 775 Plasmid R91 expression One-carbon metabolism host specificity, 1159 methanogenic bacteria, 75 Pseudomonas, 1159 short-term fixation products of '4CO2 and '4CH30H Plasmid R100 incorporated into whole cells, 75 mercury resistance genes, 1084 Ornithine formation phage lambda carrying plasmid DNA, 1084 X SUBJECT INDEX J. BACTERIOL. Plasmid Ti Psoralen treatment Agrobacterium, 1178 E. coli, 538 A. tumefaciens, 775 Purine anabolism and cell change DNA homology, 1178 M. xanthus, 582 octopine degradation, 775 Purple photosynthetic bacteria Pneumococcus cytochrome c, 1018 eclipse complex protein, 548 transformation, 548 Quinolinate biosynthesis Polymerase III activity E. coli, 136 E. coli mutants, 125 intermediate in, 136 postreplication DNA repair, 125 Polypeptide, cell-surface Receptor-mediated resistance to colicin E3, bypass of Arthrobacter, 359 E. coli, 1189 kinetics of accumulation, 359 Reductase Polyphosphates E. coli riboflavin biosynthesis, 657 M. xanthus, 1058 Repair replication patch size 32P incorporation, 1058 E. coli recL152 mutant, 444 Polysphondylium violaceum Restriction endonucleases folic acid inactivation, 955 gliding bacteria, 708 Postreplication DNA repair survery procedure, 708 E. coli mutants, 125 rffmutants Proline transport S. typhimurium, 227 E. coli mutants, 5 uridine diphosphate N-acetylmannosaminuronic Propanediol pathway acid biosynthesis, 227 E. coli, 522 Rhizobium meliloti progressive evolution, 522 catabolite-repression-like phenomenon, 1197 1,3-Propane sultone Rhodopseudomonas capsulata alkylation of transforming DNA, 854 nitrogenase, effect of light on, 201 Prophage Mucts62 Rhodopseudomonas sphaeroides E. coli deletions, induction of, 477 chromatophores, 730 fB-Propiolactone membrane vesicles, 730 alkylation of B. subtilis transforming DNA, 854 whole cells, 730 Protease Rhodospirillum rubrum B. amyloliquefaciens, 24 cytochrome c, 1018 mRNA pool, modulation of, 24 Rhodotorulapilimanae Protein degradation iron uptake and rhodotorulic acid, 69 S. cerevisiae cell cycle arrest signal, 234 Rhodotorulic acid and iron uptake Proteins, low-molecular-weight R. pilimanae, 69 B. megaterium spore germination, degraded during, rho gene product 331 E. coli histidine operon, 1201 Proteins, outer membrane Riboflavin biosynthesis N. meningitidis, 1127 deaminase, 657 Protein synthesis E. coli, 657 ribosomes from C. burnetii and E. coli, 429 reductase, 657 Proton-translocase mutants Ribosomal subunits, stable E. coli, 570 B. cereus spores, 209 isolation method, 570 Ribosomes Pseudomonas C. burnetii, 429 plasmid R91, 1159 E. coli, 429 Pseudomonas aeruginosa Rickettsia prowazeki glycerol catabolism, 638 catalase action, 452 hexosephosphate derivatives, metabolism of, 638 hydrogen peroxide production, 452 outer membrane, 381 phospholipid composition, 175 permeability barrier, 381 RNA polymerase mutations Pseudomonas aeruginosa PAT B. subtilis sporulation, 983 linkage map, 507 RNA-ribose metabolism Pseudomonas aeruginosa trp cells, transfornants of B. bacteriovorus grown on E. coli, 936 deletion plasmids, 312 enzyme activity from the gene on the plasmid, 312 Saccharomyces cerevisiae Pseudomonas fluorescens actinomycin D-sensitive mutants, 142 E. coli arginine genes expressed in, 1187 aromatic amino acid pathway, mutations in, 55 Pseudomonas glycinea cell cycle arrest signal, 234 linkage analysis, 497 fructose-1,6-bisphosphatase, 647 plasmids R68, R68.45, and R68.185, 497 inositol-less death, 49 VOL. 136, 1978 SUBJECT INDEX xi membrane damage, 49 suppression, 983 nuclear pore diameters in Gl-arrested cells, 318 Squalene peptide transport-deficient mutant, 1174 C. dehydrogenans, 614 phosphofructokinase, 647 Staphylococcus aureus protein degradation, 234 cell wall of methicillin-resistant strains, 976 sporulation, 55 metalloprotease, 607 sterol-steryl ester interconversion, 531 molecular cloning vehicles, 402 superkiller mutants, 1002 Sterols vacuole permeability, 234 C. dehydrogenans, 614 Saccharomyces telluris interconversion with steryl esters, 531 thermal adaptation, 815 S. cerevisiae, 531 Sacculus Steryl esters E. coli, 723 interconversion with free sterols, 531 glycan chain arrangement, 723 S. cerevisiae, 531 Salmonella typhimurium Streptococcal transformation yield amino acid uptake in mutants, 588 proteolytic enzymes, enhancement by, 295 atsA of K. aerogenes, 714 Streptococcus faecalis glutamine synthetase levels in mutants, 588 autolytic enzyme system and temperature, 491 histidine transport genes, 1094 lysis, 491 maltose induction of outer membrane protein, 286 Streptococcus lactis mutant selection, 1094 glycolysis, 465 outer membrane protein induced by maltose, 286 sugar:phosphotransferase system, 465 rff mutants, 227 Streptococcus mutans tRNA methylase, 191 ,8-galactosidase and lactose-P-enolpyruvate PTS, tynA of K. aerogenes, 714 co-induction of, 900 uridine diphosphate N-acetylmannosaminuronic Streptococcus salivarius acid biosynthesis, 227 B-galactosidase and lactose-P-enolpyruvate PTS, Schizosaccharomyces pombe co-induction of, 900 acid phosphatase and growth during cell cycle, 558 Succinic dehydrogenase mutants D-Serine deaminase synthesis B. subtilis, 304 dsdC activator, 96 crossed immunoelectrophoresis, 304 E. coli, 96, 104 Sugar:phosphotransferase system small-molecule regulation, 104 S. lactis, 465 Serratia marcescens Superkiller Saccharomyces cerevisiae mutants, 1002 tryptophan synthase 82 protein, 790 Serum Temperature effects on lysis A. ramosus L-forms, reversion of, 565 S. faecalis, 491 Small-molecule regulation Thermal adaptation of yeasts, 815 E. coli, 104 Thiovulum sp., 765 D-serine deaminase synthesis, 104 Tn3 mutants Spheroplast-like structures complementation, 742 pesticin, 786 Tn5 (Kan) Yersinia, 786 absence of DNA sequences homologous to, 419 Spiroplasma citri E. coli, 419 lipids, 829 tonB region Spore germination E. coli, 1165 L-alanine receptor site, hydrophobic character of, Torulopsis austromarina 484 thermal adaptation, 815 B. megaterium, 331 Torulopsis bovina B. subtilis, 484 thermal adaptation, 815 inhibition by hydrophobic compounds, 484 Torulopsis pintolopesii low-molecular-weight proteins, 331 thermal adaptation, 815 Spore outgrowth Torulopsis psychrophila B. subtilis, 111 thermal adaptation, 815 DNA synthesis, 111 Transformation Spores eclipse complex protein, 548 B. cereus, 209 pneumococcus, 548 B. megaterium, 433 Transformation yield ribosomal subunits, 209 proteolytic enzymes, enhancement by, 295 Sporulation streptococcus, 295 aromatic amino acid pathway, mutations in, 55 Transforming DNA benzeneboronic acid, 625 alkylation, 854 B. subtilis, 625, 983 B. subtilis, 854 RNA polymerase mutations, 983 Transport, autoenergized S. cerevisiae, 55 E. coli, 867 xii SUBJECT INDEX J. BACTERIOL. D-lactate, 867 K. aerogenes, 714 Trichophyton mentagrophytes regulation in hybrids, 714 arthrosporulation, 1120 carotenogenesis, 1120 Uracil uptake Trichosporon cutaneum E. coli, 839 4-hydroxyphenylacetic acid degradation, 449 Ureaplasma urealyticum tRNA methylase cell fractions, 833 S. typhimurium, 191 enzymatic activities, 833 Trypanosoma Ureotelism ammonotelism, 1184 Blastocrithidia, 1184 Trypsin peptide patterns Crithida, 1184 tryptophan synthase 82 protein among four species Leishmania, 1184 of the Enterobacteriaceae, 790 Leptomonas, 1184 Tryptophan analogs Uridine diphosphate N-acetylmannosaminuronic acid tryptophan operon derepression by indole-3-pro- biosynthesis pionic acid, 219 S. typhimurium rffmutants, 227 E. coli, 219 Uridylic acid biosynthesis Tryptophan operon A. variabilis, 682 derepression by indole-3-propionic acid, 219 UV radiations of different energies E. coli, 219 inactivation of bacteria, 437 Tryptophan synthase 12 protein E. aerogenes, 790 E. carotovora, 790 Vacuole permeability E. coli, 780 S. cerevisiae cell cycle arrest signal, 234 S. marcescens, 790 Vesicles, spheroplast-derived membrane trypsin peptide patterns, 790 R. sphaeroides, 730 Turnover Vitamin B12 receptor B. subtilis cell wall material, 886 E. coli, 1050 Tunicamycin resistance synthesis, repression of, 1050 B. subtilis mutants, 818 locations of mutational sites, 818 Yersinia tynA pesticin, 786 K. aerogenes, 714 spheroplast-like structures, 786 regulation in hybrids, 714 Yersinia pestis Tyramine oxidase gene (tynA) aspartase deficiency, 757 JOURNAL

OF

BACTERIOLOGY

VOLUME 136

WASHINGTON, D C 20006 1978 JOURNAL OF BACTERIOLOGY

VOLUME 136 * 1978 EDITORIAL BOARD Simon Silver, Editor-in-Chief (1982) Washington University, St. Louis. Mo. Stanley C. Holt, Editor (1982) Donald P. Nierlich, Editor (1982) University of Massachusetts, Amherst University of California, Los Angeles Sam Kaplan, Editor (1983) Allen T. Phillips, Editor (1980) University of Illinois, Urbana Pennsylvania State University, University Park, Pa. Elizabeth McFall, Editor (1980) Howard V. Rickenberg, Editor (1983) New York University, New York, N. Y. National Jewish Hospital, Denver, CO Mark Achtman (1979) Wolfgang Epstein (1978) Eugene W. Nester (1978) James N. Adams (1979) David P. Fan (1978) Hiroshi Nikaido (1981) Nina Agabian (1980) Walton L. Fangman (1978) John H. Nordin (1979) James Akagi (1979) Gerald R. Fink (1978) Michio Oishi (1980) David Apirion (1979) W. R. Finnerty (1979) Ronald H. Olsen (1978) Arthur 1. Aronson (1979) John D. Foulds (1979) Sunil Palchaudhuri (1979) Gad Avigad (1980) Bijan K. Ghosh (1981) Charles Panos (1978) Stephen D. Barbour (1979) Harry E. Gilleland, Jr. (1979) Leo Parks (1979) Jeffrey M. Becker (1980) Helen Greer (1980) Martin Pato (1978) Claire M. Berg (1980) Walter R. Guild (1978) William S. Reznikoff (1979) Douglas E. Berg (1980) Tadayo Hashimoto (1979) Palmer Rogers (1978) Richard S. Berk (1980) Gerald L. Hazelbauer (1978) Antonio H. Romano (1979) Harriet Bernheimer (1980) George Hegeman (1980) Barry P. Rosen (1980) Edwin Boatman (1980) Joy Hochstadt (1980) Robert Rownd (1980) Winfried Boos (1979) Bruce Holloway (1979) Harold L. Sadoff (1980) H. D. Braymer (1979) Philip Hylemon (1979) Milton H. Saier, Jr. (1979) Jean Brenchley (1980) Joseph Inselburg (1978) Gene A. Scarborough (1979) George H. Brownell (1980) Robert J. Kadner (1979) Irwin H. Segel (1979) Richard 0. Burns (1980) Donald L. Keister (1980) Jane K. Setlow (1978) A. M. Chakrabarty (1980) Jost Kemper (1978) J. A. Shapiro (1979) Peter J. Chapman (1979) David E. Kennell (1980) David Sherratt (1980) G. William Claus (1979) Bruce C. Kline (1980) Douglas W. Smith (1980) Don B. Clewell (1980) Thomas G. Lessie (1979) Issar Smith (1978) Stanley N. Cohen (1979) Robert L. Lester (1978) Lucile Smith (1978) Ronald S. Cole (1980) Hillel S. Levinson (1978) Dieter G. Soil (1979) S. F. Conti (1978) K. Brooks Low (1979) A. L. Sonenshein (1980) Stephen Cooper (1978) Calvin S. McLaughlin (1978) Dwight Stinnett (1979) John W. Costerton (1980) P. T. Magee (1980) Stanley Streicher (1980) John E. Cronan, Jr. (1980) Michael H. Malamy (1978) Robert L. Switzer (1979) Lolita Daneo-Moore (1981) Manley Mandel (1978) Bonnie M. Tyler (1978) Dana L. Diedrich (1980) Robert E. Marquis (1979) James A. Wechsler (1978) Walter J. Dobrogosz (1979) Neil H. Mendelson (1978) David White (1980) Patrick R. Dugan (1980) Sally Ann Meyer (1981) Clifford A. Woolfolk (1979) A. Eisenstark (1979) Gene E. Michaels (1980) Howard Zalkin (1979) Alan D. Elbein (1979) Robert A. Day, Managing Editor Gisella Pollock, Assistant Managing Editor Linda Illig, Production Editor 1913 I St., N. W., Washington, D. C. 20006 EX OFFICIO Edwin H. Lennette, President (1978-1979) Willis A. Wood, President-Elect (1978-1979) J. Mehsen Joseph, Secretary Brinton M. Miller, Treasurer

The Journal of Bacteriology, a publication of the American Made in the United States of America. Society for Microbiology, 1913 1 St., N.W., Washington, D.C. Copyright i 1978, American Society for Microbiology. 20006, is devoted to the advancement and dissemination of All Rights Reserved. fundamental knowledge concerning bacteria and other microor- ganisms. Instructions to Authors are published in the January issue each year. The Journal is published monthly, and the twelve numbers are divided into four volumes per year. The The code at the top of the first page ofan article in thisjournal nonmember subscription price is $115 per year. Single copies indicates the copyright owner's consent that copies of the article are $10. The member subscription price is $22 per year. Corre- may be made for personal use, or for personal use of specific spondence relating to subscriptions, reprints, defective copies, clients. This consent is given on the condition, however, that the availability of back issues, lost or late proofs, disposition of copier pay the stated per-copy fee through the Copyright Clear- submitted manuscripts, and general editorial matters should be ance Center, Inc., P.O. Box 765, Schenectady, New York 12301, directed to the ASM Publications Office, 1913 I St., N.W., for copying beyond that permitted by Sections 107 or 108 of the Washington, D.C. 20006 (area 202 833-9680). U.S. Copyright Law. This consent does not extend to other kinds ofcopying, such as copying for general distribution, for advertis- Second class postage paid at Washington, D.C. 20006, and at ing or promotional purposes, for creating new collective works, additional mailing offices. or for resale. Volume 136 Contents for October 1978 Number 1

Morphology and Ultrastructure Detergent-Resistant Variants of Bacillus subtilis with Reduced Cell Diameter. MICHAEL J. TILBY ...... 10 Cell Envelope and Shape of Escherichia coli Multiple Mutants Missing the Outer Membrane Lipoprotein and Other Major Outer Membrane Proteins. INGEBORG SONNTAG, HEINZ SCHWARZ, YUKINORI HIROTA, AND ULF HENNING* .280 Altered Nuclear Pore Diameters in Gl-Arrested Cells of the Yeast Saccharomyces cerevisiae. J. H. MARTIN WILLISON* AND GERALD C. JOHNSTON .318 Crossed Immunoelectrophoresis, in the Presence of Tween 20 or Sodium Deoxy- cholate, of Purified Membrane Proteins from Acholeplasma laidlawii. KARL- ERIK JOHANSSON* AND HENRI WROBLEWSKI .324 Genetics and Molecular Biology Proline Transport Carrier-Defective Mutants of Escherichia coli K-12: Properties and Mapping. KIYOTO MOTOJIMA, ICHIRO YAMATO, AND YASUHIRO ANRAKU* 5 Modulation of an Apparent mRNA Pool for Extracellular Protease in Bacillus amyloliquefaciens. R. O'CONNOR, W. H. ELLIOTT, AND B. K. MAY* .24 Effect of Mutation in the Aromatic Amino Acid Pathway on Sporulation in Saccharomyces cerevisiae. G. LuCCHINI,* A. BIRAGHI, M. L. CARBONE, A. DE SCRILLI, AND G. E. MAGNI .. 55 Role of the dsdC Activator in Regulation of D-Serine Deaminase Synthesis. MARIA C. HEINCZ AND ELIZABETH MCFALL* .96 Role of Small Molecules in Regulation of D-Serine Deaminase Synthesis. MARIA C. HEINCZ AND ELIZABETH MCFALL* ...... 104 Suppressors of a Genetic Regulatory Mutation Affecting Isoleucine-Valine Biosyn- thesis in Escherichia coli K-12. JEANETTE E. HAHN AND DAVID H. CALHOUN* 117 Reduction of Postreplication DNA Repair in Two Escherichia coli Mutants with Temperature-Sensitive Polymerase III Activity: Implications for the Postrep- lication Repair Pathway. ROBERT C. JOHNSON .125 Evidence for an Intermediate in Quinolinate Biosynthesis in Escherichia coli. FRANK D. WICKS, SHIGEKI SAKAKIBARA, AND R. K. GHOLSON* .136 Isolation and Characterization of an Actinomycin D-Sensitive Mutant of Saccha- romyces cerevisiae. CHARLES GORENSTEIN,* KATHARINE D. ATKINSON, AND ERNEST V. FALKE ...... 142 Genetic Separation of High- and Low-Affinity Transport Systems for Branched- Chain Amino Acids in Escherichia coli K-12. JAMES J. ANDERSON AND D. L. OXENDER* .. 168 Chromosome Replication During the Division Cycle in Slowly Growing, Steady- State Cultures of Three Escherichia coli B/r Strains. H. E. KUBITSCHEK* AND C. N. NEWMAN .179 Effect of Tryptophan Analogs on Derepression of the Escherichia coli Tryptophan Operon by Indole-3-Propionic Acid. ROBERT J. PAULEY, WALTER W. FRED-

RICKS, AND OLIVER H. SMITH* ...... 219 6-Cyanopurine, a Color Indicator Useful for Isolating Mutations in the nif (Nitrogen Fixation) Genes of Klebsiella pneumoniae. DOUGLAS MACNEIL AND WIN- STON J. BRILL ... 247 Fine-Structure Mapping and Complementation Analysis of nif (Nitrogen Fixation) Genes in Klebsiella pneumoniae. TANYA MACNEIL, DOUGLAS MACNEIL, GARY P. ROBERTS, MARK A. SUPIANO, AND WINSTON J. BRILL* .253

* Asterisk refers to person to whom inquiries regarding the paper should be addressed.

xv xni CONTENTS Regulation and Characterization of Protein Products Coded by the nif (Nitrogen Fixation) Genes of Kiebsiella pneumoniae. GARY P. ROBERTS, TANYA MAcNEIL, DoUGLAS MAcNEIL, AND WINSTON J. BRILL 6...... 267 Enhancement of Streptococcal Transformation Yield by Proteolytic Enzymes. P. G. FuCHS* AND W. T. DOBRZANSKI.2.5...... 295 Characterization of Succinic Dehydrogenase Mutants of Bacillus subtilis by Crossed Immunoelectrophoresis. BLANKA RUTBERG, LARs HEDERSTEDT, ERIK HOLMGREN, AND LARs RUTBERG* ...... 304 Deletion Plasmids from Transformants of Pseudomonas aeruginosa trpCells with the Hybrid Plasmid and High Levels of Enzyme Activity from the GeneRSF1001-trpon the Plasmid. KENJi NAGAHARI...... 312 Nuclear Division Cycle in Germinating Conidia of Neurospora crassa. LETICIA SERNA AND DAVID STADLER*...... 341 Cloned DNA Fragment Specifying Major Outer Membrane Protein a in Escherichia coli K-12. RANDALL C. GAYDA* AND ALVIN MARKOVITZ ...... 369 Characterization and Construction of Molecular Cloning Vehicles Within Staphy- lococcus aureus. C. R. WILSON* AND J. N. BALDWIN .402 Survey ofExtrachromosomal DNA Found in Filamentous Cyanobacteria. ROBERT D. SIMON...... 4.4...... 414 Absence of DNA Sequences Homologous to Transposable Element Tn5 (Kan) in the Chromosome of Escherichia coli K-12. DOUGLAS E. BERG* AND MARTIN DRUMMOND .... 419 Bacteriophage Mu-Induced Modification of DNA Is Dependent upon a Host Function. H. KHATOON* AND A. I. BUKHARI...... 423 Comparison of Ribosomes from Coxiella burnetii and Escherichia coli by Gel Electrophoresis, Protein Synthesis, and Immunological Techniques. 0. G. BACA ...... 429 Interactions Between UV Radiation of Different Energies in the Inactivation of Bacteria. REX M. TYRRELL* AND M. J. PEAK...... 437 Lack of Lysogenic Induction in "Diaminopimelic Acid Spheroplasts." ADRIANA BAILONE* AND ALAIN LEVINE...... 441 Dimer Excision and Repair Replication Patch Size in a recL152 Mutant of Esche- richia coli K-12. ROBERT H. ROTHMAN ...... 444 The F Plasmid May Cosegregate with Either DNA Strand of the Escherichia coli Chromosome. PAUL J. LEIBOWITZ AND MAURICE S. Fox* .455 Enhancement ofNitrosoguanidine Mutagenesis by Chloramphenicol in Escherichia coli K-12. ROBERT SKLAR...... 460 Physiology and Metabolism Role ofthe Escherichia coli Aromatic Amino Acid Aminotransferase in Leucine Biosynthesis. JANET T. POWELL AND JOHN F. MORRISON* ...... 1 ATP Hydrolysis and Synthesis by the Membrane-Bound ATP Synthetase Complex of Methanobacterium thermoautotrophicum. HANS J. DODDEMA,* THEO J. HUTTEN, CHRIS VAN DER DRIFT, AND GODFRIED D. VOGELS ...... 19 Iron in Neisseria meningitidis: Minimum Requirements, Effects of Limitation, and Characteristics of Uptake. FRED S. ARCHIBALD AND I. W. DEVOE* ... 35 Membrane Damage Associated with Inositol-Less Death in Saccharomyces cere- visiae. S. ULASZEWSKI, J. R. WOODWARD, AND V. P. CIRILLO* ...... 49 Relationship Between Phosphate Content and Immunochemical Properties of Subfractions of Bakers' Yeast Mannan. YASUHITO OKUBO, TSUTOMU ICHI- KAWA, AND SHIGEO SUZUKI* ...... 63 CONTENTS * -i Coordination Chemistry of Microbial Iron Transport Compounds: Rhodotorulic Acid and Iron Uptake in Rhodotorula pilimanae. CARL J. CARRANO AND KENNETH N. RAYMOND .. 69 One-Carbon Metabolism in Methanogenic Bacteria: Analysis of Short-Term Fixa- tion Products of 14CO2 and '4CH3OH Incorporated into Whole Cells. L. DANIELS AND J. G. ZEIKUS* ...... 75 Independence of Bacillus subtilis Spore Outgrowth from DNA Synthesis. DALIA GINSBERG AND ALEX KEYNAN* ...... 111 Arginine Biosynthesis in Neisseria gonorrhoeae: Enzymes Catalyzing the Forma- tion of Omithine and Citrulline. ELIZABETH NASH SHINNERS AND B. WESLEY CATLIN* .. 131 Heterogeneity and Distribution of Lipopolysaccharide in the Cell Wall of a Gram- Negative Marine Bacterium. JOSEPH M. DIRIENZO, CAROL F. DENEKE, AND ROBERT A. MACLEOD* .148 Composition of the Fractions Separated by Polyacrylamide Gel Electrophoresis of the Lipopolysaccharide of a Marine Bacterium. JOSEPH M. DIRIENZO, AND ROBERT A. MAcLEOD* ...... 158 Phospholipid Composition of Rickettsiaprowazeki Grown in Chicken Embryo Yolk Sacs. HERBERT H. WINKLER* AND ELIZABETH T. MILLER .175 Effect of Light on Nitrogenase Function and Synthesis in Rhodopseudomonas capsulata. JACQUES MEYER,* BRUCE C. KELLEY, AND P. M. VIGNAIS . 201 Isolation of Stable Ribosomal Subunits from Spores of Bacillus cereus. R. M. KIERAS, R. A. PRESTON, AND H. A. DOUTHIT* .. 209 Biosynthesis of Uridine Diphosphate N-Acetylmannosaminuronic Acid in rff Mu- tants of Salmonella typhimurium. HENRY C. LEW, HIROSHI NIKAIDO, * AND P. HELENA MAKELA . 227 Control of Vacuole Permeability and Protein Degradation by the Cell Cycle Arrest Signal in Saccharomyces cerevisiae. ROBERTA SUMRADA AND TERRANCE G. COOPER* .. 234 Major Outer Membrane Protein in Salmonella typhimurium Induced by Maltose. E. T. PALVA ...... 286 Purification and Characterization of Additional Low-Molecular-Weight Basic Pro- teins Degraded During Germination of Bacillus megaterium Spores. PETER SETLOW .331 Microbial Oxidation of Methane and Methanol: Isolation of Methane-Utilizing Bacteria and Characterization of a Facultative Methane-Utilizing Isolate. RA- MESH N. PATEL,* CHING T. HOU, AND A. FELIX .352 Kinetics of Accumulation of a Photodynamically Induced Cell-Surface Polypeptide in a Species of Arthrobacter. J. KENNETH HOOBER .359 Outer Membranes of Gram-Negative Bacteria. XIX. Isolation from Pseudomonas aeruginosa PAO1 and Use in Reconstitution and Definition of the Permeability Barrier. ROBERT E. W. HANCOCK* AND HIROSHI NIKAIDO .381 Cell Envelope Alterations in Antibiotic-Sensitive and -Resistant Strains of Neis- seria gonorrhoeae. LAWRENCE F. GUYMON,* DIANA L. WALSTAD, AND P. FREDERICK SPARLING .391 Levels of Cyclic AMP in Dormant, Germinated, and Outgrowing Spores and Growing Sporulating Cells of Bacillus megaterium. BARBARA SETLOW AND PETER SETLOW .. 433 Degradation of 4-Hydroxyphenylacetic Acid by Trichosporon cutaneum. VELTA L. SPARNINS, JEFFREY J. ANDERSON, JUDSON OMANS, AND STANLEY DAGLEY* 449 Absence of Hydrogen Peroxide Production or Catalase Action by Rickettsia prow- azeki. W. F. MYERS,* L. E. WARFEL, AND C. L. WISSEMAN, JR... 452 x *ii CONTENTS Enzymology Partial Purification and Properties of CTP:Phosphatidic Acid Cytidylyltransferase from Membranes of Escherichia coli. KEITH E. LANGLEY AND EUGENE P. KENNEDY* .. 85 Purification and Characterization of a tRNA Methylase from Salmonella typhi- murium. WILLIAM T. POPE AND ROBERT H. REEVES* .191 Errata Enolase from Spores and Cells of Bacillus megaterium: Two-Step Purification of the Enzyme and Some of Its Properties. RAVENDRA P. SINGH AND PETER SETLOW .463 Tryptophan Biosynthesis in Saccharomyces cerevisiae: Control of the Flux Through the Pathway. GUISEPPE MIOZZARI, PETER NIEDERBERGER, AND RALF HUTTER .463

Volume 136 Contents for November 1978 Number2 Morphology and Ultrastructure Synthesis and Salvage of Purines During Cellular Morphogenesis of Myxococcus xanthus. WEN-CHERNG TSAI AND CARL A. WESTBY* ...... 582 Arrangement of Glycan Chains in the Sacculus of Escherichia coli. R. W. H. VERWER, N. NANNINGA,* W. KECK, AND U. SCHWARZ ...... 723 Comparison, by Freeze-Fracture Electron Microscopy, of Chromatophores, Spher- oplast-Derived Membrane Vesicles, and Whole Cells of Rhodopseudomonas sphaeroides. MARY ANN JORDAN LOMMEN AND JON TAKEMOTO* .. 730 Physiological and Morphological Observations on Thiovulum sp. CARL 0. WIRSEN AND HOLGER W. JANNASCH* .765 Pesticin-Dependent Generation of Osmotically Stable Spheroplast-Like Struc- tures. PRUDENCE J. HALL AND ROBERT R. BRUBAKER* .. 786 Isolation and Characterization of Caulobacter crescentus Flagellar Hooks. CARL LAGENAUR, MARC DEMARTINI, AND NINA AGABIAN* ...... 795 Plant Microbiology Linkage Arnalysis of Pseudomonas glycinea. D. W. FULBRIGHT AND J. V. LEARY* 497 Coordinated Regulation of Octopine Degradation and Conjugative Transfer of Ti Plasmids in Agrobacterium tumefaciens: Evidence for a Common Regulatory Gene and Separate Operons. PIETER M. KLAPWIJK,* TRUDY SCHEULDER- MAN, AND ROBERT A. SCHILPEROORT ...... 775 Comparison of Lipids from Spiroplasma citri and Corn Stunt Spiroplasma. K. R. PATEL, P. F. SMITH,* AND W. R. MAYBERRY ...... 829 Genetics and Molecular Biology Stimulation of Deletions in the Escherichia coli Chromosome by Partially Induced Mucts62 Prophages. M. FAELEN* AND A. ToUSSAINT...... 477 Chromosomal Location of a Gene (nmpA) Involved in Expression of a Major Outer Membrane Protein in Escherichia coli. JOHN FoULDS* AND TUU-JYI CHAI 501 Linkage Map of Pseudomonas aeruginosa PAT. J. M. WATSON AND B. W. HOLLOWAY* ...... 507 Repair of Cross-Linked DNA and Survival of Escherichia coli Treated with Psoralen and Light: Effects of Mutations Influencing Genetic Recombination and DNA Metabolism. RICHARD R. SINDEN* AND RONALD S. COLE . 538 CONTENTS X1X Transformation in Pneumococcus: Protein Content of Eclipse Complex. D. A. MORRISON .. 548 Insertion of Bacteriophage Lambda into the deo Operon of Escherichia coli K-12 and Isolation of Plaque-Forming Xdeo+ Transducing Bacteriophages. R. S. BUXTON,* KARIN HAMMER-JESPERSEN, AND TONNY DEDENROTH HANSEN 668 Role of Lipopolysaccharide and Outer Membrane Protein of Escherichia coli K-12 in the Receptor Activity for Bacteriophage T4. NORIHIRO MUTOH, HIDEHIKO FURUKAWA, AND SHOJI MIZUSHIMA* ...... 693 Mechanism of Colicin E3 Production in Strains Harboring Wild-Type or Mutant Plasmids. MICHELE MOCK* AND MAXIME SCHWARTZ ...... 700 Restriction Endonucleases: General Survey Procedure and Survey of Gliding Bac- teria. HUBERT MAYER* AND HANS REICHENBACH .708 Genetic Mapping of Tyramine Oxidase and Arylsulfatase Genes and Their Regu- lation in Intergeneric Hybrids of Enteric Bacteria. YOSHIKATSU MUROOKA,* TADASHI HIGASHIURA, AND TOKUYA HARADA ...... 714 Analysis of Sequences Transposed by Complementation of Two Classes of Trans- position-Deficient Mutants of Tn3. RONALD GILL,* FRED HEFFRON, GORDON DOUGAN, AND STANLEY FALKOW ...... 742 Chromosome-Membrane Association in Bacillus subtilis. IV. Further Purification of DNA-Membrane Complex by Using a Combination of Centrifugation and Electrophoresis. HIROo TOYODA, KAZUO YAMAGUCHI, AND HIROSHI YOSHI- KAWA ...... 799 Plasmids of Incompatibility Group P Code for the Capacity to Propagate Bacterio- phage IKe. ROBERT B. GRANT,* MARY H. WHITELEY, AND AINA J. SHAP- LEY ...... 808 Tunicamycin-Resistant Mutants and Chromosomal Locations of Mutational Sites in Bacillus subtilis. SHINTARO NOMURA,* KUNIO YAMANE, TAKASHI SASAKI, MAKARI YAMASAKI, GAKUZO TAMURA, AND BUNJI MARUO ...... 818 Orotic Acid Excretion in Some Wild-Type Strains of Escherichia coli K-12. JOHN E. WOMACK AND GERARD A. O'DONOVAN* .. 825 Physiology and Metabolism In Vivo Regulation of Glycolysis and Characterization of Sugar:Phosphotransferase Systems in Streptococcus lactis. JOHN THOMPSON .. 465 Inhibition of Bacillus subtilis Spore Germination by Various Hydrophobic Com- pounds: Demonstration of Hydrophobic Character of the L-Alanine Receptor Site. YOKO YASUDA-YASAKI,* SACHIYo NAMIKI-KANIE, AND YOETSU HACH- ISUKA ...... 484 Effects of Temperature on the Autolytic Enzyme System of Streptococcus fae- calis. E. TERNOVE HINKS, L. DANEO-MOORE,* AND S. BRAVERMAN ... 491 Evolution of Propanediol Utilization in Escherichia coli: Mutant with Improved Substrate-Scavenging Power. ANDREW J. HACKING, JUAN AGUILAR, AND E. C. C. LIN* .. 522 Metabolic Interconversion of Free Sterols and Steryl Esters in Saccharomyces cerevisiae. F. R. TAYLOR* AND L. W. PARKS .531 Relationship Between Extraceilular Enzymes and Cell Growth During the Cell Cycle of the Fission Yeast Schizosaccharomyces pombe: Acid Phospha- tase. MACHIKO MIYATA* AND! HISAO MIYATA .... 558 Effects of Magnesium, Calcium, and Serum on Reversion of Stable L-Forms. A. H. HORWITZ AND L. E. CASIDA, JR.* .. 565 Method for Isolation of Escherichia coli Mutants with Defects in the Proton- Translocating Sector of the Membrane Adenosine Triphosphatase Com- plex. ROBERT H. FILLINGAME,* KATHY KNOEBEL, AND ANN E. WOPAT .... 570 xx CONTENTS Salmonella typhimurium LT-2 Mutants with Altered Glutamine Synthetase Levels and Amino Acid Uptake Activities. VICKY L. FUNANAGE, PETER D. AYLING, SUSAN M. DENDINGER, AND JEAN E. BRENCHLEY* .. 588 Carbon Monoxide Oxidation by Clostridium thermoaceticum and Clostridium formicoaceticum. GABRIELE B. DIEKERT AND RUDOLF K. THAUER* . 597 Occurrence of Squalene and Sterols in Cellulomonas dehydrogenans (Arnaudi 1942) comb. nov. Hester 1971. OWEN B. WEEKS* AND MARGARET DAY FRANCESCONI ..... 614 Benzeneboronic Acid Selectively Inhibits Sporulation of Bacillis sub- tilis. KATHLEEN DAvIs-MANCINI, ISMAEL P. LOPEZ, AND JAMES H. HAGE- MAN * ...... 625 Cell Division During Nutritional Upshifts of Escherichia coli. A. LOEB, B. E. McGRATH, J. M. NAVRE, AND 0. PIERUCCI* ..... 631 Relationship Between Catabolism of Glycerol and Metabolism of Hexosephosphate Derivatives by Pseudomonas aeruginosa. H. E. HEATH* AND ELIZABETH T. GAUDY ...... 638 Biosynthesis and Regulation of Fructose-1,6-Bisphosphatase and Phosphofructo- kinase in Saccharomyces cerevisiae Grown in the Presence of Glucose and Gluconeogenic Carbon Sources. JAMES J. FOY AND J. K. BHATTA- CHARJEE* ...... 647 Regulation of Uridylic Acid Biosynthesis in the Cyanobacterium Anabaena var- iabilis. THOMAS C. CURRIER AND C. PETER WOLK* ...... 682 Classes of Anabaena variabilis Mutants with Oxygen-Sensitive Nitrogenase Activ- ity. JOHN F. HAURY AND C. PETER WOLK* ... 688 Consequences of Aspartase Deficiency in Yersiniapestis. LAWRENCE A. DREYFUS AND ROBERT R. BRUBAKER* ...... 757 Hydrolysis of Phosphatidylethanolamine and Phosphatidylglycerol by Outer Mem- brane Phospholipase A from Acinetobacter sp. HO1-N. R. E. TORREGROSSA, R. A. MAKULA, AND W. R. FINNERTY* ...... 803 Changes in Composition of Envelope Proteins in Adenylate Cyclase- or Cyclic AMP Receptor Protein-Deficient Mutants of Escherichia coli. RIKIZo AONO,* MAKARI YAMASAKI, AND GAKUZO TAMURA ...... 812 Thermal Adaptation in Yeast: Obligate Psychrophiles Are Obligate Aerobes, and Obligate Thermophiles are Facultative Anaerobes. KENNETH WATSON,* HE- LEN ARTHUR, AND HEATHER MORTON ...... 815 Potentiometric Titration of the High- and Low-Potential 4Fe-4S* Centers of Azo- tobacter vinelandii Ferredoxin I. DUANE C. YOCH* AND ROBERT P. CARITH- ERS ...... 822 Enzymology Role of a Metalloprotease in Activation of the Precursor of Staphylococcal Pro- tease. GABRIEL R. DRAPEAU ...... 607 Presence in Escherichia coli of a Deaminase and a Reductase Involved in Biosyn- thesis of Riboflavin. ROBERT B. BURROWS AND GENE M. BROWN* ...... 657 Trypsin Peptide Patterns of Tryptophan Synthase #2 Protein Among Four Species of the Enterobacteriaceae. EDWARD F. BRENNAN AND VICTOR ROCHA* .... 790 Errata Specificity and Regulation of y-Aminobutyrate Transport in Escherichia coli. S. KAHANE, R. LEVITZ, AND Y S HALPERN...... 832 Effect of Substitution of Monovalent Anions in External Medium on the Swimming Pattern of Salmonella typhimurium. SHIGERU HOSOI AND FUMIO OOSAWA 832 Induction of Collagenase Production in Vibrio B-30. JOSEPH H. DREISBACH AND JOSEPH R. MERKEL ...... 832 CONTENTS Volume 136 Contents for December 1978 Number 3 Morphology and Ultrastructure Cell Envelope Associations of Aquaspirillum serpens Flagella. J. W. COULTON AND R. G. E. MURRAY* ...... 1037 Carotenogenesis Associated with Arthrosporulation of Trichophyton mentagro- phytes. TADAYO HASHIMOTO,* JORDAN H. POLLACK, AND HAROLD J. BLU- MENTHAL ...... 1120 Heat-Modifiable Outer Membrane Proteins of Neisseria meningitidis and Their Organization Within the Membrane. CARL E. FRASCH* AND Louis F. MOCCA 1127 Plant Microbiology Tumor-Inducing (Ti) Plasmids of Agrobacterium Share Extensive Regions of DNA Homology. MARTIN H. DRUMMOND AND MARY-DELL CHILTON* ...... 1178 Catabolite-Repression-Like Phenomenon in Rhizobium meliloti. DAVID S. UCKER AND ETHAN R. SIGNER* .1197 Genetics and Molecular Biology Isolation of Escherichia coli Mutants with Changed Regulation of Uracil Uptake. ROBERT FAST ...... 839 Alterations in Bacillus subtilis Transforming DNA Induced by ,B-Propiolactone and 1,3-Propane Sultone, Two Mutagenic and Carcinogenic Alkylating Agents. Z. 0. KUBINSKI* AND H. KUBINSKI ...... 854 Nitrogen Source Regulates Glutamine Synthetase mRNA Levels in Neurospora crassa. FEDERICO SANCHEZ, MIGUEL CAMPOMANES, CARMEN QUINTO, WIL- HELM HANSBERG, JAIME MORA, AND RAFAEL PALACIOS* ...... 880 Multiple Genes Coding for Octopine-Degrading Enzymes in Agrobacterium. ALICE L. MONTOYA, LARRY W. MOORE, MILTON P. GORDON, AND EUGENE W. NESTER* ... 909 Metabolism of RNA-Ribose by Bdellovibrio bacteriovorus During Intraperiplasmic Growth on Escherichia coli. ROBERT B. HESPELL* AND D. A. ODELSON ... 936 Regulation of lac Operon Expression: Reappraisal of the Theory of Catabolite Repression. BARRY L. WANNER, RYOJI KODAIRA, AND FREDERICK C. NEID- HARDT*...... 947 Suppression of Temperature-Sensitive Sporulation of a Bacillus subtilis Elongation Factor G Mutant by RNA Polymerase Mutations. HIROHIKO HIROCHIKA AND YASUO KOBAYASHI *...... 983 Kasugamycin-Dependent Mutants of Escherichia coli. ERIC R. DABBS ...... 994 Chromosomal Superkiller Mutants of Saccharomyces cerevisiae. AKlo TOH-E,* PATRICIA GUERRY, AND REED B. WICKNER .. 1002 dnaT, Dominant Conditional-Lethal Mutation Affecting DNA Replication in Esch- erichia coli. CYNTHIA A. LARK, JALIL RIAZI, AND KARL G. LARK* ...... 1008 Properties of Lambda Transducing Bacteriophages Carrying R100 Plasmid DNA: Mercury Resistance Genes. WALTER B. DEMPSEY,* SARAH A. MCINTIRE, NEIL WILLETTS, JANET SCHOTTEL, THOMAS G. KINSCHERF, SIMON SILVER, AND W. ALLEN SHANNON, JR... 1084 Duplications of Histidine Transport Genes in Salmonella typhimurium and Their Use for the Selection of Deletion Mutants. GIOVANNA FERRO-LUZZI AMES,* DONALD P. BIEK, AND ELENA NEGRI SPUDICH ...... 1094 malB Region in Escherichia coli K-12: Specialized Transducing Bacteriophages and First Restriction Map. C. MARCHAL, J. GREENBLATT, AND M. HOFNUNG* 1109 ii* CONTENTS Isolation and Characterization of Extragenic Suppressor Strains of Corynebacte- rium diphtheriae. P. BACHA* AND J. R. MURPHY ...... 1135 Morphology of an Escherichia coli Mutant with a Temperature-Dependent Round Cell Shape. MAKOTO IWAYA,* ROBERT GOLDMAN, DONALD J. TIPPER, B. FEINGOLD, AND JACK L. STROMINGER ... 1143 An Explanation for the Apparent Host Specificity of Pseudomonas Plasmid R91 Expression. G. A. JACOBY,* R. WEIss, T. R. KORFHAGEN, V. KRISHNAPILLAI, A. E. JACOB, AND R. W. HEDGES ...... 1159 HindII and HindIII Restriction Maps of the attcp80-tonB-trp Region of the Esche- richia coli Genome, and Location of the tonB Gene. KATHLEEN POSTLE AND WILLIAM S. REZNIKOFF* ...... 1165 Expression of Escherichia coli K-12 Arginine Genes in Pseudomonas fluorescens. M. MERGEAY,* A. BOYEN, C. LEGRAIN, AND N. GLANSDORFF .1187 Bypass of Receptor-Mediated Resistance to Colicin E3 in Escherichia coli K- 12. MICHAEL TILBY, INGRID HINDENNACH, AND ULF HENNING* . 1189 Construction of a Hybrid Bacteriophage-Plasmid Recombinant DNA Vector. DANIEL J. DONOGHUE* AND PHILLIP A. SHARP .. 1192 Effects of Altered rho Gene Product on the Expression of the Escherichia coli Histidine Operon. ROBERT P. LAWTHER AND G. WESLEY HATFIELD* . 1201 Differential Effect of Hydroxyurea on the Replication of Plasmid and Chromosomal DNA in Bacillus subtilis. A. G. SHIVAKUMAR AND DAVID DUBNAU* . 1205 Physiology and Metabolism Cell Fractions and Enzymatic Activities of Ureaplasma urealyticum. NINO Ro- MANO* AND ROSARIA LA LICATA ... 833 Energy Supply for Active Transport in Anaerobically Grown Escherichia coli. JOHANNES BOONSTRA, J. ALLAN DOWNIE, AND WIL N. KONINGS* .... 844 Mechanism of Auto-Energized Transport and Nature of Energy Coupling for D- Lactate in Escherichia coli. SHYUE YUE KANG .. 867 Induction and Regulation of Neuraminidase Synthesis in Arthrobacter sialophi- lUs. PHILIP WANG, DOROTHY SCHAFER, CARL A. MILLER, STUART W. TA- NENBAUM, AND MICHAEL FLASHNER* .....874 Cell Wall Assembly in Bacillus subtilis: Location of Wall Material Incorporated During Pulsed Release of Phosphate Limitation, Its Accessibility to Bacterio- phages and Concanavalin A, and Its Susceptibility to Turnover. A. J. ANDER- SON, R. S. GREEN, A. J. STURMAN, AND A. R. ARCHIBALD* .886 Co-Induction of fl-Galactosidase and the Lactose-P-Enolpyruvate Phosphotransfer- ase System in Streptococcus salivarius and Streptococcus mutans. I. R. HAMILTON* AND G. C. Y. Lo ..... 900 Dissimilatory Reduction of Bisulfite by Desulfovibrio vulgaris. H. L. DRAKE AND J. M. AKAGI* .. 916 Specificity of Nucleoside Transport in Neurospora crassa. DEBRA DUNAWAY- MARIANO AND JANE M. MAGILL* . 924 Changes in Macromolecular Synthesis and Nucleoside Triphosphate Levels During Glycerol-Induced Growth Stasis of Escherichia coli. PAULA E. HENNEN, HARRY B. CARTER, AND WILLIAM D. NUNN* ...... 929 Inactivation of the Chemoattractant Folic Acid by Cellular Slime Molds and Identification ofthe Reaction Product. PAULINE PAN* AND BERND WURSTER 955 Isolation and Characterization of Glycerol-3-Phosphate Dehydrogenase-Defective Mutants of Neurospora crassa. PATRICK F. DENOR AND JAMES B. COURTRIGHT * ...... 960 CONTENTS * * . Constancy of Growth on Simple and Complex Media. C. HOUSTON WANG AND ARTHUR L. KoCH* .969 Cell Wall Composition and Associated Properties of Methicillin-Resistant Staphy- lococcus aureus Strains. BRIAN J. WILKINSON,* KENNETH J. DORIAN, AND L. D. SABATH ...... 976 Isolation and Characterization of a Membrane-Bound, Low-Potential c-Type Cy- tochrome from Purple Photosynthetic Bacteria, with Special Reference to Rhodospirilluim rubrum. DUANE C. YOCH, ROBERT P. CARITHERS, AND DAN- IEL I. ARNON* ...... 1018 Physiological Role and Membrane Lipid Modulation of the Membrane-Bound (Mg", Na+)-Adenosine Triphosphatase Activity in Acholeplasma laidlawii. DAVID C. JINKS, JOHN R. SILVIUS, AND RONALD N. MCELHANEY* .1027 Repression of Synthesis of the Vitamin B12 Receptor in Escherichia coli. ROBERT J. KADNER ...... 1050 Incorporation of 32Pi into Nucleotides, Polyphosphates, and Other Acid-Soluble Compounds by Myxococcus xanthus During Myxospore Formation. P. Y. MAEBA* AND R. SHIPMAN ...... 1058 A Second Transport System for sn-Glycerol-3-Phosphate in Escherichia coli. MANFRED ARGAST, DOUGLAS LUDTKE, THOMAS J. SILHAVY, AND WIN- FRIED Boos* ... 1070 Isolation of a Peptide Transport-Deficient Mutant of Yeast. R. MARDER, B. ROSE, J. M. BECKER, AND F. NAIDER* .... 1174 Ureotelism and Ammonotelism in Trypanosomatids. NOBUKO YOSHIDA AND E. PLESSMANN CAMARGO* ... 1184 Errata Cystine Reductase in the Dimorphic Fungus Histoplasma capsulatum. BRUNO MARESCA, ERIC JACOBSON, GERALD MEDOFF, AND GEORGE KOBAYASHI .... 1208 Relationship of R6K Replicating Forms to the Folded Chromosome of Escherichia coli. ERROL R. ARCHIBOLD, CLARENCE W. CLARK, AND RONALD J. SHEEHY 1208 INDEX TO DATE OF ISSUE

Month Date of Issue Pages

October 16 October 1978 1-463 November 9 November 1978 465-832 December 8 December 1978 833-1208