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Mitf from Neural Crest to Melanoma: Signal Transduction and Transcription in the Melanocyte Lineage

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Mitf from Neural Crest to Melanoma: Signal Transduction and Transcription in the Melanocyte Lineage Downloaded from genesdev.cshlp.org on September 25, 2021 - Published by Cold Spring Harbor Laboratory Press REVIEW Mitf from neural crest to melanoma: signal transduction and transcription in the melanocyte lineage Colin R. Goding1 Eukaryotic Transcription Laboratory, Marie Curie Research Institute, The Chart, Oxted Surrey, RH8 OTL UK Perhaps the most obvious manifestation of the complex ferentiation-specific functions such as the genesis of pig- interplay between environmental cues, signal transduc- ment, or more interestingly from the developmental tion pathways, and transcription factors underlying the perspective, any step from the specification and commit- genesis and differentiation of a cell lineage is provided by ment to the melanocyte lineage, to the survival, prolif- the enormous diversity of genetically determined pig- eration, and migration of melanoblasts from the neural mentation patterns. Because melanocytes, the cells re- crest. sponsible for skin, hair, and eye color, are dispensable for Interest in the melanocyte system is also afforded by viability, the highly visible consequences of mutations the impact on melanocytes of ultraviolet (UV) light. In affecting the melanocyte lineage (Fig. 1) has allowed over humans, epidermal melanocytes respond to UV irradia- 90 independent genes to be implicated in the genesis of tion by increasing the synthesis of melanin in mem- mouse coat color (Mouse Genome Informatics: http:// brane-bound structures termed melanosomes that are www.informatics.jax.org/). The 30 or so of these genes transferred to surrounding keratinocytes, a process that have been cloned to date (http://www.cbc.umn. known as the tanning response. At least one role for edu/ifpcs/micemut.htm) not only encode proteins re- melanin is likely to be in protection against UV-induced quired for the manufacture of the pigment melanin and DNA damage (Hill 1992). The incidence of UV-induced the function of the melanosome, but also encode signal- DNA damage, however, correlates with the risk of trans- ing molecules and transcription factors that play critical formation of the melanocyte to a malignant melanoma, roles in the development of the melanocyte lineage. This an aggressive and increasingly common form of cancer unusually large genetic resource which extends to a wide for which a proportion of the population have a genetic variety of species has made the regulation of melanocyte predisposition. The difficult path towards understanding development an attractive system for understanding the molecular and genetic basis of the progression from a how the coordination of gene expression required for the melanocyte to a malignant melanoma has been made commitment and differentiation of a specific cell lineage easier with recent observations that link the genesis of is achieved. melanoma to deregulation of pathways operating in me- Melanocytes originate as nonpigmented precursors lanocyte development. termed melanoblasts in the mouse neural crest at around The aim of this review is to provide an overview of embryonic day 10.5 (E10.5), and following migration and how gene expression in neural crest-derived melanocytes proliferation reach the limb buds by E12 and enter the is controlled by the interplay between specific transcrip- epidermis at the level of the lateral trunk by E13/E14 tion factors and signal transduction pathways. Because (Mayer 1973). Melanocytes are found as mature pigment of limitations of space, the review focuses on the regu- cells in the skin and hair follicles, as well as a range of lation of the expression and function of the Microphthal- other sites where their role as pigment-producing cells is mia-associated transcription factor, Mitf. For the same less understood. Thus, melanocytes are present in the reason, the development of the retinal pigment epithe- choroid layer of the eye, the Harderian gland, the anal lium (RPE), the pigmented layer of cells lying immedi- canal, and in the stria vascularis of the inner ear where ately behind the retina which arises via differentiation of they are crucial for hearing because they play a vital role the posterior part of the optic cup, is not discussed. in the endolymph-controlled generation of action poten- tials (Steel and Barkway 1989; Tachibana 1999). The ef- fects of mutations affecting genes playing a key role in The microphthalmia-associated transcription factor the melanocyte lineage may therefore be manifest at sev- eral levels: Mutations may affect genes required for dif- Ten years ago, virtually nothing was known about how the program of gene expression underlying the genesis of the melanocyte lineage was regulated. The isolation of 1E-MAIL [email protected]; FAX +44 1883 714375. the genes encoding the melanocyte-specific melanogenic 1712 GENES & DEVELOPMENT 14:1712–1728 © 2000 by Cold Spring Harbor Laboratory Press ISSN 0890-9369/00 $5.00; www.genesdev.org Downloaded from genesdev.cshlp.org on September 25, 2021 - Published by Cold Spring Harbor Laboratory Press Melanocyte development identification of melanocyte-specific transcription fac- tors that regulated the expression of these differentiation markers and, in the long term, would yield insights into the regulation of these genes by signal transduction path- ways. Moreover, because tyrosinase, Tyrp-1, and the other melanogenic enzymes act together within the melanosome to generate pigment, it was anticipated that their expression would be coordinated by transcription factors binding to elements shared between their respec- tive promoters, and that at least some of these transcrip- tion factors might also play a role in the development of the melanocyte lineage. The analysis of the tyrosinase and Tyrp-1 promoters appeared to fulfill this prophecy. Both promoters shared a common 11-bp element AGT- CATGTG termed the M box (Lowings et al. 1992), which was required for their expression. Importantly, the core CATGTG (E box) motif within the M box was known to bind members of the basic-helix-loop-helix (bHLH) and bHLH-leucine zipper (bHLH-LZ) families of transcrip- tion factors. The identification of the M box as an ele- ment conserved in melanocyte-specific promoters was complemented by the isolation of the gene encoding the Microphthalmia-associated bHLH-LZ transcription fac- tor Mitf (Hodgkinson et al. 1993; Hughes et al. 1993). The isolation of this gene, which will be referred to Figure 1. Mutations in the genes encoding key signaling mol- throughout as Mitf irrespective of species of origin, was ecules or transcription factors result in characteristic coat color a turning point in understanding the molecular mecha- phenotypes. (A) An E12.5 transgenic embryo expressing LacZ nisms underlying melanocyte development. under control of the Dct promoter. In addition to strong staining Mice bearing null alleles of the Mitf gene exhibit a loss in the telencephalon, Dct-positive melanoblasts migrating of neural crest-derived melanocytes and associated deaf- away from the neural crest are readily apparent. (B)AMitfmi-bw ness, and a failure of RPE differentiation (Moore 1995), mouse characterized by an insertion of an L1 line element in while in the zebrafish a null mutation in an Mitf gene, intron 3 of the Mitf gene specifically disrupts expression of the nacre, results in loss of melanophores but does not affect Mitf–M isoform (Yajima et al. 1999) leading to loss of neural the RPE (Lister et al. 1999), perhaps implying the exis- crest-derived melanocytes. As the Mitf isoforms expressed in tence of additional Mitf-like genes in this species. The the RPE function normally, the outcome is a black-eyed white phenotype. (C)AnMitf vga-9/mi-sp compound heterozygote re- importance of Mitf as a determining factor in melano- sulting from a cross between a Vga-9 homozygote (a null mutant cyte development was underlined when it was shown for Mitf) and an Mitf mi-sp homozygote. Vga-9 homozygotes are that expression of Mitf in fibroblasts conferred on the white, whereas the heterozygotes have normal pigmentation. recipient cells characteristics of melanocytes (Tachibana Both Mitf mi-sp homo- and heterozygotes are pigmented nor- et al. 1996), although the fibroblasts used in this experi- mally despite the fact that this allele prevents expression of the ment were unusual in that they were able to express the Mitf(+) isoform. In the compound heterozygote shown, the Dct (Tyrp-2) gene even before ectopic expression of Mitf. white patches result from loss of melanoblasts and reveal a Similarly, ectopic expression of Mitf can induce trans- requirement for the expression of the Mitf(+) isoform in this differentiation of neuroretina into melanocytes (Planque genetic background. The cross was performed in the laboratory et al. 1999), and missexpression of zebrafish Mitf re- of Heinz Arnheiter. (D)APax3 heterozygote exhibiting a char- acteristic white belly patch. (E)ASox10Dom+/−/Mitf mi+/− double sulted in ectopic melanized cells (Lister et al. 1999). heterozyogote. Neither Sox10Dom heterozyogotes nor Mitfmi Thus, it would appear that in some cells at least the heterozygotes exhibit any marked pigmentation phenotype. In expression of Mitf is sufficient to establish a program of contrast, enhanced loss of melanocytes resulting in extensive melanocyte-specific gene expression. hypopigmentation is observed in the double heterozygote The Mitf gene that spans over 50 kb in the mouse shown (Potterf et al. 2000), reflecting the ability of Sox10 to (Hallsson et al. 2000; Udono et al. 2000) encodes a posi- regulate Mitf
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