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Binding of Nonsteroidal Anti-Inflammatory Drugs to DPPC: Structure and Thermodynamic Aspects

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Binding of Nonsteroidal Anti-Inflammatory Drugs to DPPC: Structure and Thermodynamic Aspects 4132 Langmuir 2008, 24, 4132-4139 Binding of Nonsteroidal Anti-inflammatory Drugs to DPPC: Structure and Thermodynamic Aspects Marlene Lu´cio,*,†,⊥ Frank Bringezu,‡,|,⊥ Salette Reis,† Jose´ L. F. C. Lima,† and Gerald Brezesinski§,⊥ REQUIMTE, Faculdade de Farma´cia, UniVersidade do Porto, Rua Anı´bal Cunha, 4099-030 Porto, Portugal, Institute of Medical Physics and Biophysics, UniVersity of Leipzig, Ha¨rtelstrasse 16, D-04103 Leipzig, Germany, and Max-Planck Institute of Colloids and Interfaces, Research Campus Golm, Am Mu¨hlenberg 1, D-14476 Potsdam, Germany ReceiVed NoVember 16, 2007. In Final Form: January 8, 2008 The effect of nonsteroidal anti-inflammatory drugs (NSAIDs) on the phase transition and phase properties of 1,2-dipalmitoylphosphatidylcholine (DPPC) has been investigated in both 2D (monolayers at the air/water interface) and 3D (multilayers in lipid/water dispersions) model systems. The 2D membrane models have been characterized by means of pressure-area isotherms and grazing incidence X-ray diffraction (GIXD) measurements. Differential scanning calorimetry (DSC) and simultaneous small- and wide-angle X-ray diffraction have been applied to lipid aqueous dispersions. All NSAIDs studied altered the main transition temperature of the gel to liquid-crystalline phase transition, with the arylacetic acid derivatives (acemetacin and indomethacin) showing the largest effects. A comparison of the results reveals distinct structural features of the membrane models after interaction with the NSAID. All drugs induced perturbations in the lipid liquid-crystalline phase, suggesting a major change in the hydration behavior of DPPC. Again, the largest effects on the structural parameters are found for the arylacetic acid derivatives. The results obtained in the different model systems give indications of the role of the membrane/NSAID interactions that might also be important for NSAID gastric injury. Introduction serious side effects during medical applications will also depend Cyclooxygenase (COX), also known as prostaglandin synthase on the understanding of the processes initiating and promoting (PGH), is a potent mediator of inflammation.1 Nonsteroidal anti- gastric injury. Such mechanisms are complex, and the cascade inflammatory drugs (NSAIDs) bind to cyclooxygenase, thereby of events leading to mucosal damage must therefore be inhibiting the production of prostaglandins. COX-1 and COX-2 characterized and can also be related to the topical irritancy of are two isoforms of cyclooxygenase.2 COX-1 is a constitutive NSAIDs. Davenport suggested that the normal resistance of the enzyme that produces gastroprotective prostaglandins,3 and gastric mucosa to back diffusion of luminal acid can be disrupted COX-2 is induced by cytokines, mitogens, and endotoxins in by the topical administration of lipid-soluble damaging agents 8 inflammatory cells, which are responsible for the production of such as acetylsalicylic acid or aspirin. Evidence of the direct inflammatory prostaglandins.4 Most of the NSAIDs bind at the superficial damaging effects of other drugs that are members of active sites of both COX-1 and COX-2 with little specificity and the NSAID family was subsequently provided by many inves- lead to side effects such as gastric lesions and renal toxicity. tigators who showed histological, biochemical, and permeability 9-11 The discovery by Vane and colleagues provided new insight changes in the gastric mucosa. However, the “barrier- into both the mechanisms of NSAID therapeutic activities and breaking” activity of the drugs has not been established on a side effects1,2,5-7 such as the development of inhibitors that molecular basis. selectively bind to COX-2, which would be expected to show Despite the complexity of the acid resistance properties of the anti-inflammatory action in vivo with minimal gastric side effects. gastric inner surface, the extracellular lining of surfactant-like However, the sequence of events resulting from cyclooxygenase phospholipids on the surface within the mucus gel layer represents inhibition does not completely explain the overall gastric toxicity an initial line of defense of the stomach and confers hydrophobic, 12 of NSAIDs. The development of novel NSAIDs showing less non-wettable, acid-resistant properties to the mucosa. NSAIDs appear to decrease mucosal hydrophobicity because * Corresponding author. E-mail: [email protected]. Fax: +351-222078961. of their ability to suppress prostaglandin synthesis. In addition, Tel: +351-222078966. they may chemically associate with phospholipids and destabilize † Universidade do Porto. them from the mucus gel layer.13 Such a transition would increase ‡ University of Leipzig. the wettability of the stomach and result in an increase in the § Max-Planck Institute of Colloids and Interfaces. | Current address: Institute of Biotechnology, Martin Luther University, back-diffusion of luminal acid into the mucosa; consequently, Halle-Wittenberg, Kurt-Mothes Strasse 3, 06120 Halle, Germany. the development of erosions must be expected. The aim of the ⊥ These authors have contributed equally. (1) Vane, J. R. Nature 1971, 231, 232-235. (8) Davenport, H. W. Gastroenterology 1964, 46, 245-253. (2) Vane, J. R. Nature 1994, 367, 215-216. (9) Giraud, M. N.; Motta, C.; Romero, J. J.; Bommelaer, G.; Lichtenberger, (3) Mitchell, J. A.; Akarasereenont, P.; Thiemrman, C.; Flower, R.; Vane, J. L. M. Biochem. Pharmacol. 1999, 57, 247-254. R. Proc. Natl. Acad. Sci. U.S.A. 1994, 90, 11693-11697. (10) Morris, G. P.; Wallace, J. L.; Harding, P. L.; Krasse, E. J.; Idle, S. T. Dig. (4) Herschman, H. R. Biochim. Biophys. Acta 1996, 1299, 125-140. Dis. Sci. 1984, 29,6-11. (5) Vane, J. R. Am. J. Med. 1998, 104, 2S-8S. (11) Wallace, J. L. Gastroenterology 1997, 112, 1000-1016. (6) Vane, J. R.; Botting, R. M. Inflammation Res. 1995, 44,1-10. (12) Lichtenberger, L. M.; Graziani, L. A.; Dial, E. J.; Butler, B. D.; Hills, B. (7) Vane, J. R.; Botting, J.; Botting, R. M. ImproVed Nonsteroidal Anti A. Science 1983, 219, 1327-1329. Inflammatory Drugs-COX-2 Enzyme Inhibitors; Kluwer Academic Publishers: (13) Lichtenberger, L. M.; Wang, Z. M.; Romero, J. J.; Ulloa, C.; Perez, J. London, 1996. C.; Giraud, M. N.; Barreto, J. C. Nat. Med. 1995, 1, 154-158. 10.1021/la703584s CCC: $40.75 © 2008 American Chemical Society Published on Web 03/13/2008 Binding of NSAIDs to DPPC Langmuir, Vol. 24, No. 8, 2008 4133 Figure 1. Chemical structures of the NSAIDs investigated: 1-(p-chlorobenzoyl)-5-methoxy-2-methylindole-3-acetic acid carboxymethyl ester (1, acemetacin), 1-(4-chlorobenzoyl)-5-methoxy-2-methyl-3-indoleacetic acid (2, indomethacin), and N-(4-nitro-2-phenoxyphenyl)- methanesulfonamide (3, nimesulide). present study was therefore to investigate the ability of NSAIDs drug adsorption and penetration into the lipid film present at the to bind to or to penetrate into layers of surface-active phos- interface. Structural changes within the DPPC monolayer upon pholipids. The results may elucidate the effects of these drugs adsorption of indomethacin were investigated by synchrotron compromising the integrity of the gastric mucosal barrier. For X-ray experiments at grazing incidence (GIXD). These experi- this purpose, three different NSAIDs (indomethacin, acemetacin, ments were carried out to obtain information on the structure of and nimesulide; Figure 1) were selected, and their influence on the DPPC monolayer interacting with indomethacin. The effect the biophysical properties of 1,2-dipalmitoylphosphatidylcholine of NSAIDs on the lipid organization in 2D monolayers and 3D (DPPC) was studied by different complementary experimental bilayers are investigated and compared. techniques in 2D and 3D model systems. DPPC is a typical phospholipid with regard to its role in Experimental Methods - determining the physical chemical and biological properties of Materials. DPPC, indomethacin, acemetacin, and nimesulide were cellular membranes.14 By dispersing this lipid in water, multi- obtained from Sigma Chemical Co. (St. Louis, MO), and used without lamellar vesicles are spontaneously formed that exhibit many further purification. All other chemicals were purchased from Merck features of the cellular membranes (e.g., the barrier and transport (Darmstadt, Germany). Solutions were prepared with HEPES buffer function or permeability by passive diffusion through the bilayer). (10 mM, 0.1 M NaCl, pH 7.4). Water was purified with a Millipore In aqueous dispersions, DPPC exhibits a rich polymorphism that desktop system, leading to a specific resistance of 18.2 MΩ cm. 19 encompasses crystalline, gel, and liquid-crystalline phases. The Methods. Following the classical thin film hydration method, formation of these structures depends on the environmental the effects of NSAIDs in multilamellar liposomes (MLV) of DPPC were studied by comparing the thermotropic behavior of the conditions (e.g., temperature, pressure, salt conditions, vesicle 14-16 drug homogeneously dissolved with DPPC in an organic solvent preparation, and hydration). According to this, in the current (direct mixing procedure) with that observed by leaving a fixed work differential scanning calorimetry measurements (DSC) were amount of drug in contact with already-prepared vesicles (incubation applied for the thermoanalysis of the phase behavior of the lipid procedure). aqueous dispersions and the effect of the NSAID on the phase- Incubation Procedure. DPPC was dissolved in a chloroform- transition parameters. The results are compared with structural methanoI
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