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Anthracene Derivatives in Some Species of Rumex L Vol. 76, No. 2: 103-108, 2007 ACTA SOCIETATIS BOTANICORUM POLONIAE 103 ANTHRACENE DERIVATIVES IN SOME SPECIES OF RUMEX L. GENUS MAGDALENA WEGIERA1, HELENA D. SMOLARZ1, DOROTA WIANOWSKA2, ANDRZEJ L. DAWIDOWICZ2 1 Departament of Pharmaceutical Botany Skubiszewski Medical University of Lublin Chodki 1, 20-039 Lublin, Poland e-mail: [email protected] 2 Faculty of Chemistry, Marii Curie-Sk³odowska University of Lublin (Received: June 1, 2006. Accepted: September 5, 2006) ABSTRACT Eight anthracene derivatives (chrysophanol, physcion, emodin, aloe-emodin, rhein, barbaloin, sennoside A and sennoside B) were signified in six species of Rumex L genus: R. acetosa L., R. acetosella L., R. confertus Willd., R. crispus L., R. hydrolapathum Huds. and R. obtusifolius L. For the investigations methanolic extracts were pre- pared from the roots, leaves and fruits of these species. Reverse Phase High Performance Liquid Chromatography was applied for separation, identification and quantitative determination of anthracene derivatives. The identity of these compounds was further confirmed with UV-VIS. Received data were compared. The roots are the best organs for the accumulation of anthraquinones. The total amount of the detected compo- unds was the largest in the roots of R. confertus (163.42 mg/g), smaller in roots R. crispus (25.22 mg/g) and the smallest in roots of R. hydrolapathum (1.02 mg/g). KEY WORDS: Rumex sp., roots, fruits, leaves, anthracene derivatives, RP-HPLC. INTRODUCTION scion-anthrone, rhein, nepodin, nepodin-O-b-D-glycoside and 1,8-dihydroxyanthraquinone from R. acetosa (Dedio The species belonging to the Rumex L. genus are wide- 1973; Demirezer and Kuruuzum 1997; Fairbairn and El- spread in the world. Twenty five of these species grow in Muhtadi 1972a; He et al. 1981; Hsiao et al. 1980; Kato and Poland. The roots from five of them: R. alpinus L., R. cri- Morita 1987; Sharma and Ragaswami 1977; Tamano and spus L., R. hydrolapathum Huds., R. obtusifolius L. and R. Koketsu 1982; Varma et al. 1984); chrysophanol, emodin, patientia L., which deliver material named Radix Lapa- aloe-emodin, nepodin, rumicin and rhein from R. acetosel- thi, are used in phytotherapy due to their laxative proper- la (Dedio 1973; Fairbairn and El-Muhtadi 1972a; Hegnau- ties. Other two species (R. acetosa L. and R. acetosella L.) er 1973; Kaczmarek and Urszulak 1964; Martinod et al. are components of our diet (Mi³kowska et al. 1997; Wegie- 1978; Mowszowicz 1976); chrysophanol, physcion, emo- ra and Smolarz 2005). din, aloe-emodin, nepodin, nepodin-1-O-b-glycoside, chry- The latest research report on antioxidant, antibacterial, sophanein, frangulin and rhein from R. confertus (Bagrii antiviral, antifungal, antimutagenic, antitumor and immu- and Kurmaz 1966; Broda and Mowszowicz 1996; Dedio nosuppressive activities of some species of Rumex L. ge- 1973; Fairbairn and El-Muhtadi 1972a; Hegi 1981; Hoppe nus (Lee et al. 2005; Wegiera and Smolarz 2005; Yildirim 1975; Kaczmarek and Urszulak 1964; Sayed et al. 1974; et al. 2001). Tétényi 1970); chrysophanol, physcion, emodin and their The present study comprises phytochemical investigations glycosides, rhein, nepodin, nepodin-1-O-b-D-glycoside, on anthracene derivativatives of six species of Rumex L. 1.5-dihydroxyanthraquinone, oxymethyloanthraquinone Derivatives of anthracene as secondary metabolites im- and glucofranguline B from R. crispus (Dedio 1973; Demi- portant for these plants have been chromatographically de- rezer 1994; Fairbairn and El-Muhtadi 1972a; Gunaydin et tected and isolated. There are the following compounds: al. 2002; He et al. 1981; Hegi 1981; Kaczmarek and chrysophanol, emodin, physcion and their 8-O-b-D-gluco- Urszulak 1964; Leveau and Durand 1969; Midiwo and Ro- pyranosides, physcion-1-O-b-D-glucopyranoside, aloe- kunga 1985; Sayed et al. 1974; Tétényi 1970); chrysopha- emodin as aloe-emodin w-acetate, emodin-anthrone, phy- nol, chrysophanol-anthron, physcion, physcion-anthron, 104 ANTHRACENE DERIVATIVES IN RUMEX L. Wegiera M. et al. TABLE 1. Structure, UV-VIS spectra and RT-values of anthracene derivativates identified in the Rumex species. aloe-emodin, emodin and chrysophanol-, physcion- and Muhtadi 1972a, b; He et al. 1981; Hegi 1981; Hsiao et al. emodin-glycosides from R. hydrolapathum (Dedio 1973; 1980; Hoppe 1975; Sayed et al. 1974; Kaczmarek and Fairbairn and El-Muhtadi 1972a; Hegi 1981; Labadie et al. Urszulak 1964). 1972); emodin, chrysophanol, physcion, aloe-emodin, rhe- However, there are no reports on comparative investiga- in, nepodin, frangulin-emodin, frangulin-emodin-glycoside tions of these therapeutically active compounds in the ro- and nepodin-8-glycoside from R. obtusifolius (Arellano ots, leaves and fruits of the above mentioned species. 1998; Brazdova et al. 1969; Dedio 1973; Fairbairn and El- Vol. 76, No. 2: 103-108, 2007 ACTA SOCIETATIS BOTANICORUM POLONIAE 105 MATERIAL AND METHODS with Rheodyne automated injection valve to which 25 µl sample loop was connected; gradient pump (GP50); absor- Plant material bance detector (AD25); photodiode array detector Fruits, leaves and roots of six species from Rumex L. ge- (PDA100). All analyses were under control of PeakNet6 nus: R. acetosa L., R. acetosella L., R. confertus Willd., R. data acquisition system. crispus L., R. hydrolapathum Huds. and R. obtusifolius L. Chromatographic separations were carried out at 25°C were used in this study. using Prodigy ODS-3 column (5 µm, 250×4.6 mm I.D.) The plant materials were collected from different places: (Phenomenex, Torrance, CA, USA) and a security guard near Sandomierz and Nisko in July 2003; near S³upia Nad- column of the same factory. The column and the guard co- brze¿na and around Lublin in June 2005. lumn were placed in the oven and the analyses were perfor- med at 30°C (Column Thermostat, JetStream II Plus, Chemicals and standards Knauer, Warsaw, Poland). Methanol (HPLC grade) and glacial acetic acid (analyti- The chromatographic analyses were performed in gra- cal-reagent grade), were purchased from Polish Factory of dient elution conditions using as solvent A aqueous Chemicals (POCh Gliwice, Poland). Water was purified on CH3COOH solution containing 5 ml of glacial acetic acid a Milli-Q system from Millipore (Millipore, USA). per litre and as solvent B methanol. The following gra- Standards are as follows: chrysophanol (Aldrich), physcion dient of mobile phase (at a flow of 1 ml/min) was applied: (Roth Karlsruhe), emodin (Carl Roth Gmbh Sigma), aloe- linear increase of B from 45 to 85% (0-30 min), next 85% emodin (Roth Karlsruhe), aloin A and aloin B mixture (Carl B during 10 min and finally linear decrease of B from 85 to Roth Gmbh Sigma), rhein (Carl Roth Gmbh Sigma), sennosi- 45% during 2 min. In the end the column was conditioned de A (Roth Karlsruhe), sennoside B (Roth Karlsruhe). using the mobile phase containing 45% B (5 min). Chro- In this investigation we used 0.2-0.002% methanolic so- matograms were monitored by the AD25 detector at 254 lutions of these standards. nm and by the PDA detector and the absorbance spectra (190-750 nm) were collected continuously during the cour- Extraction se of each run. The air-dried and powdered plant materials (4 g) from fruits, leaves and roots of each of the taxons were extracted with 40 ml 80% aq. methanol and three times with 30 ml RESULTS AND DISCUSION 80% aq. methanol (15 minutes of each extraction) in super- sonic water bath in room temperature. The combined In this study we used Reversed Phase High Performance extracts were evaporated from the solvent under reduced Liquid Chromatography to separate and identify anthrace- pressure at 40°C to 4 ml and subjected to further chromato- ne derivatives in methanolic extracts from the leaves, roots graphic investigations. The rest of dried methanol extracts and fruits from six species belonging to Rumex L. genus. has been stored in the refrigerator. The mobile phase used for RP-HPLC separation of the de- tected compounds in extracts was optimized experimental- RP-HPLC analysis of the extracts ly. The retention times of the peaks in the samples were RP-HPLC measurements were performed on Dionex li- compared with authentic reference compounds. The identity quid chromatograph (Dionex Corp., Sunnyvale, CA, USA) of compounds on chromatogram was confirmed by UV-VIS consisting of: chromatography enclosure (LC20) equipped spectra (Table 1). AU UV_VIS_1 -0.700 barbaloin 9.6 -0.600 -0.500 -0.400 -0.300 -0.200 10.3 -0.100 sennoside B 4.2 sennoside A 6.2 chrysophanol 32.9 aloe-emodin 19.9 rhein 24.4 emodin 30.8 physcion 37.2 -0.020 Fig. 1. Chromatogram (RP-HPLC) obtained 1.55.0 10.0 15.0 20.025.0 30.0 35.0 40.0 from methanolic extracts from the leaves of Time [min] R. confertus. 106 ANTHRACENE DERIVATIVES IN RUMEX L. Wegiera M. et al. Fig. 2. The content and distribution of anth- racene derivatives obtained from the leaves of different species of Rumex genus (in mg/g of dry sample). Fig. 3. The content and distribution of anth- racene derivatives obtained from the roots of different species of Rumex genus (in mg/g of dry sample). Vol. 76, No. 2: 103-108, 2007 ACTA SOCIETATIS BOTANICORUM POLONIAE 107 Fig. 4. The content and distribution of anth- racene derivatives obtained from the fruits of different species of Rumex genus (in mg/g of dry sample). The qualitative investigation showed, that the detected TABLE 2. Distribution of investigated anthracene derivatives in different compounds are widespread in genus Rumex L. The leaves taxons of Rumex L. (mg/g of dry samples). of the investigated species contain a complete set of the No. Species Fruits Leaves Roots Total content identified compounds (Fig. 1 and 2), whereas sennoside of investigated B is missing in the roots of R. crispus and rhein is absent compounds [mg/g] from the roots of R.
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