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51) International Patent Classification: 94588 (US). ROSEN, Barbara; 510 Vincent Drive, Moun¬ Tain View, California 94041 (US

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51) International Patent Classification: 94588 (US). ROSEN, Barbara; 510 Vincent Drive, Moun¬ Tain View, California 94041 (US ll ( (51) International Patent Classification: 94588 (US). ROSEN, Barbara; 510 Vincent Drive, Moun¬ A01N 63/14 (2020.01) C07K 14/21 (2006.01) tain View, California 94041 (US). SCHELLENBERG- C07K 14/195 (2006.01) ER, Ute; 914 Moreno Avenue, Palo Alto, California 94303 (US). WEI, Jun-Zhi; 7250 NW 62Nd Avenue, Johnston, (21) International Application Number: Iowa 5013 1-0552 (US). XIE, Weiping; 2105 Salas Court, PCT/US20 19/064579 East Palo Alto, California 94303 (US). (22) International Filing Date: (74) Agent: BECKER, James E.; 7250 NW 62Nd Avenue, PO 05 December 2019 (05. 12.2019) Box 552, Johnston, Iowa 5013 1-0552 (US). (25) Filing Language: English (81) Designated States (unless otherwise indicated, for every (26) Publication Language: English kind of national protection av ailable) . AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (30) Priority Data: CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, 62/779,642 14 December 2018 (14. 12.2018) US DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (71) Applicant: PIONEER HI-BRED INTERNATIONAL, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, INC. [US/US]; 7100 NW 62Nd Avenue, PO Box 1014, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, Johnston, Iowa 5013 1-1014 (US). MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (72) Inventors: COY, Monique; 7250 NW 62ND Avenue, PO SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, Box 552, Johnston, Iowa 5013 1-0552 (US). GRUVER, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Steven D ; 955 Monte Verde Drive, Pacifica, California 94044 (US). KOZY, Heather; 40 Vallecito Lane, Wal¬ (84) Designated States (unless otherwise indicated, for every nut Creek, California 94596 (US). LIU, Lu; 2303 Mid- kind of regional protection available) . ARIPO (BW, GH, dlefield Road, Palo Alto, California 94301 (US). PARK, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, Young-Jun; 1589 Maple Leaf Drive, Pleasanton, California UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (54) Title: BIOLOGICALS AND THEIR USE IN PLANTS FK228 (57) Abstract: Biological strains, compositions, and methods of using the strains and compositions for reducing overall insect damage. [Continued on next page] ||| ||||| ||||| ||||| |||| 11| ||| ||||| ||||| ||||| ||||| ||||| |||| limn nil nil nil TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, Cl, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(H)) as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) Published: with international search report (Art. 21(3)) with (an) indication(s) in relation to deposited biological material furnished under Rule 13bis separately from the description (Rules 13bis.4(d)(i) and 48.2(a)(viii)) with sequence listing part of description (Rule 5.2(a)) BIOLOGIC ALS AND THEIR USE IN PLANTS CROSS-REFERENCE TO RELATED APPLICATIONS This Application claims the benefit of U.S. Provisional Application No. 62/779,642 filed on December 14, 2018, which is incorporated herein by reference in its entirety. FIELD Biological strains, compositions, and methods of using the strains and compositions for reducing overall insect damage. REFERENCE TO SEQUENCE LISTING SUBMITTED ELECTRONICALLY The official copy of the sequence listing is submitted electronically via EFS-Web as an ASCII formatted sequence listing with a file named 6593_SeqList.txt created on August 21, 2018 and having a size of 1,395 kilobytes and is filed concurrently with the specification. The sequence listing contained in this ASCII formatted document is part of the specification and is herein incorporated by reference in its entirety. BACKGROUND Certain species of microorganisms of the genus Bacillus are known to possess pesticidal activity against a range of insect pests including Lepidoptera, Diptera, Coleoptera, Hemiptera and others. Bacillus thuringiensis (Bt) and Bacillus popilliae are among the most successful biocontrol agents discovered to date. Insect pathogenicity has also been attributed to strains of B. larvae, B. lentimorbus, B. sphaericus and B. cereus. Microbial insecticides, particularly those obtained from Bacillus strains, have played an important role in agriculture as alternatives to chemical pest control. Crop plants have been developed with enhanced insect resistance by genetically engineering crop plants to produce pesticidal proteins from Bacillus. For example, corn and cotton plants have been genetically engineered to produce pesticidal proteins isolated from strains of Bt. These genetically modified crops are now widely used in agriculture and have provided the farmer with an environmentally friendly alternative to traditional insect-control methods. While they have proven to be very successful commercially, these genetically modified, insect-resistant crop plants provide resistance to only a narrow range of the economically important insect pests. In some cases, insects can develop resistance to different insecticidal compounds, which raises the need to identify alternative biological control agents for pest control. There has been a long felt need for environmentally friendly compositions and methods for controlling or eradicating insect pests of agricultural significance, i.e., methods that are selective, environmentally inert, non-persistent, and biodegradable, and that f t well into insect pest management schemes. SUMMARY Some embodiments relate to a composition comprising a Pseudomonas chlororaphis, a Burkholderia rinojensis, or a Chromobacterium haemolyticum, wherein the Pseudomonas chlororaphis, Burkholderia rinojensis, or Chromobacterium haemolyticum has insecticidal activity. In some embodiments, the methods and compositions relate to a insecticidal bacterial strain comprising a DepA gene, a DepB gene, a DepC gene, a DepF gene, a DepG gene, DepH gene, a DepE gene, or a DepD gene. In some embodiments, the DepA gene comprises an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs: 109, 121, 133, 145, 158, 171, 184, or 197. In some embodiments, the DepB gene comprises an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs: 110, 122, 134, 146, 159, 172, 185, or 198. In some embodiments, the DepC gene comprises an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs: 111, 123, 135, 147, 160, 173, 186, or 199. In some embodiments, the DepD gene comprises an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs: 112, 124, 136, 148, 161, 174, 187, or 200. In some embodiments, the DepE gene comprises an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs: 113, 125, 137, 149, 162, 175, 188, or 201. In some embodiments, the DepF gene comprises an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs: 114, 126, 138, 150, 163, 176, 189, or 202. In some embodiments, the DepG gene comprises an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs: 115, 127, 139, 151, 164, 177, 190, or 203. In some embodiments, the DepH gene comprises an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs: 116, 128, 140, 152, 165, 178, 191, or 204. In some embodiments, the Depl gene comprises an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs: 117, 129, 141, 153, 166, 179, 192, or 205. In some embodiments, the DepJ gene comprises an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs: 118, 130, 142, 154, 167, 180, 193, or 206. In some embodiments, the DepK gene comprises an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs: 143, 155, 168, 181, or 194. In some embodiments, the DepL gene comprises an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs: 119, 131, 156, 169, 182, 195, or 207. In some embodiments, the DepM gene comprises an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs: 120, 132, 144, 157, 170, 183, 196, or 208. In some embodiments, the methods and compositions relate to bacterial strains comprising a 16S RNA sequence having at least 95% identity to any one of SEQ ID NOs: 1-8. In one embodiment, the disclosure relates to a composition comprising a Pseudomonas chlororaphis strain SS532D1 (NRRL Deposit No. B-67638), wherein the Pseudomonas chlororaphis strain SS532D1 has insecticidal activity. In one embodiment, the disclosure relates to a composition comprising a Pseudomonas chlororaphis strain SSP459B9-3 (NRRL Deposit No. B-67639), wherein the Pseudomonas chlororaphis strain SS532D1 has insecticidal activity. In one embodiment, the disclosure relates to a composition comprising a Burkholderia rinojensis strain JH59 178-1 (NRRL Deposit No. B-67640), wherein the Burkholderia rinojensis strain JH59 178-1 has insecticidal activity. In one embodiment, the disclosure relates to a composition comprising a Chromobacterium haemolyticum strain JH91791-1 (NRRL Deposit No. B-67642), wherein the Chromobacterium haemolyticum strain JH91791-1 has insecticidal activity. In one embodiment, the disclosure relates to a composition comprising a Chromobacterium haemolyticum strain PMCJ4191H4-1 NRRL Deposit No.
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