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Cells Immunoglobulin-Like Receptor on Myeloid CD300LF Caspase-Independent Cell Death by CD300LF (MAIR-V), an Inhibitory Immunoglobulin-Like Receptor on Myeloid Cells This information is current as of September 26, 2021. Ismail Can, Satoko Tahara-Hanaoka, Kaori Hitomi, Takako Nakano, Chigusa Nakahashi-Oda, Naoki Kurita, Shin-ichiro Honda, Kazuko Shibuya and Akira Shibuya J Immunol 2008; 180:207-213; ; doi: 10.4049/jimmunol.180.1.207 Downloaded from http://www.jimmunol.org/content/180/1/207 References This article cites 34 articles, 12 of which you can access for free at: http://www.jimmunol.org/ http://www.jimmunol.org/content/180/1/207.full#ref-list-1 Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists by guest on September 26, 2021 • Fast Publication! 4 weeks from acceptance to publication *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2008 by The American Association of Immunologists All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. The Journal of Immunology Caspase-Independent Cell Death by CD300LF (MAIR-V), an Inhibitory Immunoglobulin-Like Receptor on Myeloid Cells1 Ismail Can, Satoko Tahara-Hanaoka, Kaori Hitomi, Takako Nakano, Chigusa Nakahashi-Oda, Naoki Kurita, Shin-ichiro Honda, Kazuko Shibuya, and Akira Shibuya2 The myeloid-associated Ig-like receptor family (CD300) consists of nine activating or inhibitory cell surface receptors pref- erentially expressed on myeloid cells and are encoded by the genes in a small cluster on mouse chromosome 11. One of the receptors, CD300LF (MAIR-V), has a long cytoplasmic tail containing two consensus ITIMs and an immunoreceptor ty- rosine-based switching motif, suggesting that CD300LF regulates the activation of myeloid cells. However, the functional characteristics of this receptor are still incompletely understood. In this study, we demonstrate that cross-linking CD300LF with anti-CD300LF mAb induced cell death in peritoneal macrophages as well as in several transfectants expressing CD300LF. CD300LF-mediated cell death was dependent on the cytoplasmic region but did not require an ITIM or immu- Downloaded from noreceptor tyrosine-based switching motif. Scanning electron microscopy revealed a loss of blebs from the surface of the dead cells mediated by CD300LF, a morphological feature similar to that observed in apoptotic cells. However, CD300LF-medi- ated cell death was not inhibited by a caspase inhibitor, N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone, or autoph- agy inhibitors, 3-methyladenine or N-acetyl-L-cystein. Moreover, the splicing isoform of a transcription factor, X-box binding protein-1, which is produced in dead cells as a response to endoplasmic reticulum stress, was not detected. Together, these results indicate that CD300LF mediates caspase and endoplasmic reticulum stress-independent cell death by a novel http://www.jimmunol.org/ mechanism. The Journal of Immunology, 2008, 180: 207–213. ctivating and inhibitory cell surface receptors play im- paired activating and inhibitory receptors. CD300a is preferen- portant roles in the regulation of immune responses (1, tially expressed on myeloid cells, including macrophages, neu- A 2). Recent progress has demonstrated that many inhibi- trophils, DCs, and bone marrow-derived cultured mast cells, tory receptors pair with activating, as well as inhibitory, isoforms and mediates an inhibitory signal through an ITIM in the cy- (2, 3), both of whose genes are located in small clusters on a toplasmic portion (7, 8). In contrast, MAIR-II, expressed on chromosome (4). The CMRF-35, recently assigned as CD300 (5), macrophages, associates with either ITAM-containing adapters by guest on September 26, 2021 are a multigene family consisting of six genes on human chromo- DAP12 or FcR␥-chain and mediates an activating signal, some 17 (6). The mouse counterparts of CD300 molecules, which resulting in inflammatory cytokine secretion from macrophages were reported to be a myeloid-associated Ig-like receptor (MAIR)3 (7, 9). (7–10)/CMRF-35-like molecule-1 (CLM) (11)/leukocyte mono- We and others have recently reported that one of the genes in the Ig-like receptor (LMIR) (12, 13)/dendritic cell (DC)-derived Ig mouse CD300 family, MAIR-IV, also named LMIR4 or CLM5 receptor (DIgR) (14, 15), were encoded by nine genes on chro- (13, 16), associates with the FcR␥-chain and mediates an activat- mosome 11, syntenic region of human chromosome 17. CD300a ing signal in neutrophils (10). The Ig-like domain of MAIR-IV in (MAIR-I) and MAIR-II (7), also named as CLM8/LMIR1 and the extracellular portion shares 91% amino acid identity with that CLM4/LMIR2/DIgR1, respectively (14, 11, 12), constitute of MAIR-V (CD300LF), also named LMIR3 or CLM1 (10, 13, 16). CD300LF has a long cytoplasmic tail containing two consen- sus ITIMs, suggesting that MAIR-IV and CD300LF constitute Department of Immunology, Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, Graduate School of Comprehensive Human paired activating and inhibitory receptors. In fact, CD300LF asso- Sciences, University of Tsukuba, Ibaraki, Japan ciates with SHP-1, and expression of CD300LF in a macrophage Received for publication July 23, 2007. Accepted for publication October 28, 2007. cell line, RAW264, effectively inhibits in vitro osteoclast forma- The costs of publication of this article were defrayed in part by the payment of page tion (11). Recently, Shi et al. (15) reported DIgR2 lacks 7 amino charges. This article must therefore be hereby marked advertisement in accordance acids (PAIQVPI) in the stalk region of CD300LF, suggesting that with 18 U.S.C. Section 1734 solely to indicate this fact. DIgR2 is a splicing variant of CD300LF. Although DIgR2 was 1 This research was supported in part by grants provided by the Ministry of Education, reported to mediate negative regulation of DC-induced Ag-specific Science, and Culture of Japan. T cell responses, whether CD300LF is also involved in the same 2 Address correspondence and reprint requests to Dr. Akira Shibuya, Department of Immunology, Institute of Basic Medical Sciences, Graduate School of Comprehen- function in DC remains undetermined. sive Human Sciences, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305- In this study, we investigated the role of CD300LF in immune 3585, Japan. E-mail address: [email protected] responses and describe that CD300LF-mediated signals induce 3 Abbreviations used in this paper: MAIR, myeloid-associated Ig-like receptor; CLM, caspase-independent cell death in myeloid cells. CMRF-35-like molecule-1; LMIR, leukocyte mono-Ig-like receptor; DC, dendritic cell; DIgR, DC-derived Ig receptor; 3-MA, 3-methyladenine; NAC, N-Acetyl-L-cys- tein; MDC, monodansylcadaverine; z-VAD-fmk, N-benzyloxycarbonyl-Val-Ala- Asp-fluoromethylketone; PI, propidium iodide; ITSM, immunoreceptor tyrosine- Materials and Methods based switching motif; WT, wild type; ER, endoplasmic reticulum; XBP-1, X-box Mice and cell lines binding protein-1; EM, electron microscope; Siglec, sialic acid-binding Ig-like lectin. C57BL/6J mice were purchased from CLEA Japan. All mice used in this Copyright © 2007 by The American Association of Immunologists, Inc. 0022-1767/07/$2.00 study were 8–10 wk of age and were maintained in our animal facility www.jimmunol.org 208 CASPASE-INDEPENDENT CELL DEATH BY CD300 (MAIR-V) anti-CD300LF anti-CD300LF under specific pathogen-free conditions. All experiments were performed A anti-Flag according to the guidelines of the animal ethics committee of the Univer- (TX70) (TX73) sity of Tsukuba Animal Research Center. BW5147, RAW264, and P3U1 cells are murine T, macrophage, and myeloma cell lines, respectively. All BW5147 these cell lines were purchased from the American Type Tissue Culture Collection. Abs and reagents Flag-CD300LF Control rat and mouse IgGs, anti-mouse CD11b (Mac1), CD11c, Ly6G (MAIR-V) (Gr-1), CD3, NK1.1 mAbs, and PE-conjugated annexin V were purchased /BW5147 from BD Biosciences; anti-Flag mAb were purchased from Sigma-Aldrich; and rabbit anti-rat IgG were purchased from Rockland. Anti-CD300LF (TX70 and TX73) and anti-Flag (TX74) mAbs were generated in our lab- Flag-CD300a ϫ 7 (MAIR-I) oratory. In brief, 1 10 BW5147 transfectants expressing Flag-tagged /BW5147 CD300LF suspended in 500 ␮l PBS was emulsified with the same volume of CFA. A rat was s.c. injected in each footpad with 500 ␮l of the sus- pension of BW5147 transfectants on day 1 and 8. On day 11, popliteal Flag-MAIR-IV lymph node cells were harvested and fused with the Sp2/0 myeloma cell /BW5147 line by a standard method. BW5147 transfectants expressing Flag-tagged CD300LF, CD300a, MAIR-II, or MAIR-IV were used for screening of specificity to CD300LF or Flag protein by flow cytometry. Wortmannin, CD300LF Flag LY294002, cytochalasin D, DTT, 3-methyladenine (3-MA), N-Acetyl-L- Downloaded from cystein (NAC), staurosphorine, etoposide, LPS, Earle’s balanced salts B B220+/Mac1-B220-/Mac1+ CD3+ Gr1hi NK1.1+ solution and monodansylcadaverine (MDC) were purchased from Sigma- Aldrich. N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (z-VAD- fmk) was purchased from Calbiochem. Zymozan and CpG DNA Spleen (ODN1826) were purchased from Molecular Probes and Invivogen, respectively. Establishment of transfectants expressing CD300LF http://www.jimmunol.org/ Bone Marrow The CD300LF cDNA tagged with the Flag cDNA at the NH2 terminus was subcloned into the pMX retroviral vector (17). To generate site-directed CD300LF mutants at residues Y241,Y289, and Y325, we designed PCR primers containing a codon for F (TTT) instead of Y (TAT).
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