Redalyc.Characterization of Phytopathogenic Fungi, Bacteria

Total Page:16

File Type:pdf, Size:1020Kb

Redalyc.Characterization of Phytopathogenic Fungi, Bacteria Revista Facultad Nacional de Agronomía - Medellín ISSN: 0304-2847 [email protected] Universidad Nacional de Colombia Colombia López Cardona, Nathali; Castaño Zapata, Jairo Characterization of Phytopathogenic Fungi, Bacteria, Nematodes and Viruses in Four Commercial Varieties of Heliconia (Heliconia sp.) Revista Facultad Nacional de Agronomía - Medellín, vol. 65, núm. 2, 2012, pp. 6703-6716 Universidad Nacional de Colombia Medellín, Colombia Available in: http://www.redalyc.org/articulo.oa?id=179925831015 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Characterization of Phytopathogenic Fungi, Bacteria, Nematodes and Viruses in Four Commercial Varieties of Heliconia (Heliconia sp.) Caracterización de Hongos, Bacterias, Nemátodos y Virus Fitopatógenos en Cuatro Variedades Comerciales de Heliconia (Heliconia sp. ) Nathali López Cardona1 and Jairo Castaño Zapata2 Abstract. Analysis of 914 samples of roots, rhizomes, Resumen. El análisis de 914 muestras de raíces, rizomas, pseudostems, inflorescences and leaves of four commercial pseudotallos, inflorescencias y hojas de cuatro variedades varieties of heliconia, cultivated at the municipality of Chinchiná– comerciales de heliconia, cultivadas en el municipio de Caldas (Colombia), allowed to identify five genera of plant Chinchiná–Caldas (Colombia), permitieron identificar cinco pathogenic fungi (Rhizoctonia, Fusarium, Colletotrichum, géneros de hongos fitopatógenos (Rhizoctonia, Fusarium, Helminthosporium and Curvularia), three genera of plant Colletotrichum, Helminthosporium y Curvularia), tres pathogenic bacteria (Ralstonia, Pseudomonas and Erwinia), géneros de bacterias fitopatógenas (Ralstonia, Pseudomonas two species of viruses (Banana streak virus (BSV, Badnavirus,) y Erwinia), dos especies de virus (Banana streak virus (BSV, and Cucumber mosaic virus (CMV, Cucumovirus,)), and Badnavirus,) y Cucumber mosaic virus (CMV, Cucumovirus,)), seven genera of plant parasitic nematodes (Helicotylenchus, y siete géneros de nematodos fitoparásitos (Helicotylenchus, Tylenchus, Meloidogyne, Ditylenchus, Aphelenchoides, Tylenchus, Meloidogyne, Ditylenchus, Aphelenchoides, Pratylenchus, and Radopholus). Of these, Fusarium sp., Pratylenchus y Radopholus). De ellos, Fusarium sp., affecting pseudostems, Pseudomonas sp., affecting leaves and afectando pseudotallos, Pseudomonas sp., afectando hojas inflorescences, and the plant parasitic nematodes Ditylenchus e inflorescencias, y los nematodos fitoparásitos Ditylenchus sp., Aphelenchoides sp., Pratylenchus sp. and Radopholus sp., Aphelenchoides sp., Pratylenchus sp. y Radopholus sp., are new records in the heliconia production in Colombia. sp., son reportes nuevos en la producción de heliconias en The most limiting diseases corresponded to leaf blight, caused Colombia. Las enfermedades más limitantes correspondieron al by Helminthosporium sp.; the bacterioses, caused by tizón foliar, causado por Helminthosporium sp.; la bacteriosis, Pseudomonas sp.; the spotted stems, caused by Fusarium causada por Pseudomonas sp.; el manchado de pseudotallos, sp.; and soft rot of the pseudostems, caused by Erwinia sp. causado por Fusarium sp.; la pudrición de calcetas, The pathogenicity tests demonstrated that Colletotrichum sp. causada por Erwinia sp.; y el moko, causado por Ralstonia and Phoma sp. are not pathogenic in leaves; while Fusarium solanacaearum. Las pruebas de patogenicidad demostraron sp., inoculated in pseudostems, Helminthosporium sp. and que Colletotrichum sp. y Phoma sp. no son patogénicos en Pseudomonas sp., inoculated in leaves, and Colletotrichum hojas; mientras que Fusarium sp., inoculado en pseudotallos, sp. and Pseudomonas sp., inoculated in inflorescences, Helminthosporium sp. y Pseudomonas sp., inoculados en had incidence values of 83.3, 86.6, 93.3, 100.0 and 100.0%, hojas, y Colletotrichum sp. y Pseudomonas sp., inoculados respectively. en inflorescencias, presentaron valores de incidencia de 83,3, 86,6, 93,3, 100,0 y 100,0%, respectivamente. Key words: Heliconiaceae, diagnosis, diseases, pathogenicity. Palabras clave: Heliconiaceae diagnóstico, enfermedades, patogenicidad. Taking under consideration the importance of dollars exported during 2008. Currently, Colombia heliconias (Heliconia sp.) cultivation in Colombia has become the first flower provider for the United as an export product and an additional alternative States, the first worldwide carnation producer- for job creation plans, research must be carried exporter and the second worldwide flower exporter out in order to determine the biotic factors limiting country after Holland, thus generating 183,000 their production. The country has an important jobs (99,000 direct jobs and 84,000 indirect jobs) competitive advantage for tropical flowers in a 7,500 ha sown field dedicated to exportation production; in more than 40 years, Colombia has cultivation, contributing 9.9% to the gross domestic increased its income going from quite a few annual product (GDP) and putting in 25% of the rural female thousand dollars to more than US $1,094 million employment in Colombia (Asocolflores, 2009). 1 Agronomic Engineer. Universidad de Caldas - Faculty of Agricultural Sciences. Phytopathology Master’s Program. Street 65 No. 26-10. Manizales, Colombia <[email protected]> 2 Titular Professor. Universidad de Caldas. Faculty of Agricultural Sciences. Phytopathology Program. Street 65 No. 26-10. Manizales, Colombia. <[email protected]> Recibido: Abril 09 de 2012; aceptado: Octubre 22 de 2012. Rev.Fac.Nal.Agr.Medellín 65(2): 6703-6716. 2012 López, N.; Castaño, J. More than 250 species of the Heliconia genus In Hawaii, Sewake and Uchida (no date) describe have been described, of which 97 are registered in that diseases caused by Bipolaris incurvata, Colombia and 48 of these have been described as Exserohilum (=Helminthosporium) rostratum, endemic species, placing the country as the biggest Pyriculariopsis sp., Mahabalella sp., Mycosphaerella biodiversity center for this genus in the world. For sp. and Rhizoctonia solani in H. bihai var. Lobster this reason, Colombia has been ranked as a strong Claw One and H. caribaea, can become very limiting competitor in the international market of these in heliconia production. Likewise, Nakati (no date) flowers. It is estimated that in the Coffee Triangle and Rabelo (2007) consider that Exserohilum sp. and the Valle del Cauca there exist approximately and Bipolaris sp. are the most destructive foliar 471 ha cultivated with heliconias and foliage, pathogens of heliconia cultivation in that state. contributing 7% of the national export. In Risaralda, the place in which the greatest heliconia sown field In Venezuela, Madríz et al. (1991) report area in the Coffee Triangle is concentrated, there that Colletotrichum musae limits heliconias are around 120 ha dedicated to this cultivation commercialization, because it produces brown with and the number of flower growers is higher than white center spots which demarcate the main 150, which represent an annual income close to US leaves vein in Heliconia caribaea, but also affects $1,500,000 (Díaz, 2006; Asocolflores, 2009). inflorescence in which, in the initial disease stages, produces irregular and sunken brown-black spots in It is common to observe that when the sown field the bracts, and in the disease advanced stage causes of cultivation becomes bigger, the presence of widespread dry decay in the whole inflorescence. diseases caused by a diversity of phytopathogenic microorganisms also increases, which is known as In this research, some of the phytopathogens affecting the price of varietal popularity (Castaño, 2002). the production in four commercial varieties of heliconias This situation is not foreign to the heliconias in the municipality of Chinchiná-Caldas (Colombia) production in Colombia. In spite of this, most were identified, as a contribution for the appropriate researchers on this species have focused in a implementation of the cultivation management variety of topics such as heliconia inventories, strategies. The study consisted of the identification, taxonomy, ecology, distribution and classification following Koch’s postulates, of pathogens affecting that have been found in the country, which has the production of four heliconia commercial varieties given up precise information and to research (H. caribaea Lam. var. Kawachi, H. caribaea Lam. var. about the pathogenic agents affecting this genera Salmon, H. lobster (L.) var. Orange Lobster and H. species and, even more, the relation they have ortotricha (L.) var. Edge of Night). The species were with climate elements such as precipitation, selected because of their commercial importance in relative humidity, and temperature. The previous, the national and international fields. has lead to the need to take measures of inappropriate handling which generate a series MATERIALS AND METHODS of environmental and economic consequences afterwards. Area of study. The research was carried out in el Rosario farm in the municipality of Chinchiná–Caldas The only published work about the identification (Colombia), at an altitude of 1,200 masl, 80% average of diseases in Colombian heliconias has been relative humidity, an average temperature of 22 ºC, developed by Villegas et al. (2005) and Alarcón
Recommended publications
  • Monilochaetes and Allied Genera of the Glomerellales, and a Reconsideration of Families in the Microascales
    available online at www.studiesinmycology.org StudieS in Mycology 68: 163–191. 2011. doi:10.3114/sim.2011.68.07 Monilochaetes and allied genera of the Glomerellales, and a reconsideration of families in the Microascales M. Réblová1*, W. Gams2 and K.A. Seifert3 1Department of Taxonomy, Institute of Botany of the Academy of Sciences, CZ – 252 43 Průhonice, Czech Republic; 2Molenweg 15, 3743CK Baarn, The Netherlands; 3Biodiversity (Mycology and Botany), Agriculture and Agri-Food Canada, Ottawa, Ontario, K1A 0C6, Canada *Correspondence: Martina Réblová, [email protected] Abstract: We examined the phylogenetic relationships of two species that mimic Chaetosphaeria in teleomorph and anamorph morphologies, Chaetosphaeria tulasneorum with a Cylindrotrichum anamorph and Australiasca queenslandica with a Dischloridium anamorph. Four data sets were analysed: a) the internal transcribed spacer region including ITS1, 5.8S rDNA and ITS2 (ITS), b) nc28S (ncLSU) rDNA, c) nc18S (ncSSU) rDNA, and d) a combined data set of ncLSU-ncSSU-RPB2 (ribosomal polymerase B2). The traditional placement of Ch. tulasneorum in the Microascales based on ncLSU sequences is unsupported and Australiasca does not belong to the Chaetosphaeriaceae. Both holomorph species are nested within the Glomerellales. A new genus, Reticulascus, is introduced for Ch. tulasneorum with associated Cylindrotrichum anamorph; another species of Reticulascus and its anamorph in Cylindrotrichum are described as new. The taxonomic structure of the Glomerellales is clarified and the name is validly published. As delimited here, it includes three families, the Glomerellaceae and the newly described Australiascaceae and Reticulascaceae. Based on ITS and ncLSU rDNA sequence analyses, we confirm the synonymy of the anamorph generaDischloridium with Monilochaetes.
    [Show full text]
  • Colletotrichum – Names in Current Use
    Online advance Fungal Diversity Colletotrichum – names in current use Hyde, K.D.1,7*, Cai, L.2, Cannon, P.F.3, Crouch, J.A.4, Crous, P.W.5, Damm, U. 5, Goodwin, P.H.6, Chen, H.7, Johnston, P.R.8, Jones, E.B.G.9, Liu, Z.Y.10, McKenzie, E.H.C.8, Moriwaki, J.11, Noireung, P.1, Pennycook, S.R.8, Pfenning, L.H.12, Prihastuti, H.1, Sato, T.13, Shivas, R.G.14, Tan, Y.P.14, Taylor, P.W.J.15, Weir, B.S.8, Yang, Y.L.10,16 and Zhang, J.Z.17 1,School of Science, Mae Fah Luang University, Chaing Rai, Thailand 2Research & Development Centre, Novozymes, Beijing 100085, PR China 3CABI, Bakeham Lane, Egham, Surrey TW20 9TY, UK and Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3AB, UK 4Cereal Disease Laboratory, U.S. Department of Agriculture, Agricultural Research Service, 1551 Lindig Street, St. Paul, MN 55108, USA 5CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands 6School of Environmental Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada 7International Fungal Research & Development Centre, The Research Institute of Resource Insects, Chinese Academy of Forestry, Bailongsi, Kunming 650224, PR China 8Landcare Research, Private Bag 92170, Auckland 1142, New Zealand 9BIOTEC Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, NSTDA, 113 Thailand Science Park, Paholyothin Road, Khlong 1, Khlong Luang, Pathum Thani, 12120, Thailand 10Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou 550006 PR China 11Hokuriku Research Center, National Agricultural Research Center,
    [Show full text]
  • Notes on Currently Accepted Species of Colletotrichum
    Mycosphere 7(8) 1192-1260(2016) www.mycosphere.org ISSN 2077 7019 Article Doi 10.5943/mycosphere/si/2c/9 Copyright © Guizhou Academy of Agricultural Sciences Notes on currently accepted species of Colletotrichum Jayawardena RS1,2, Hyde KD2,3, Damm U4, Cai L5, Liu M1, Li XH1, Zhang W1, Zhao WS6 and Yan JY1,* 1 Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, People’s Republic of China 2 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand 3 Key Laboratory for Plant Biodiversity and Biogeography of East Asia (KLPB), Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, Yunnan, China 4 Senckenberg Museum of Natural History Görlitz, PF 300 154, 02806 Görlitz, Germany 5State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China 6Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. Jayawardena RS, Hyde KD, Damm U, Cai L, Liu M, Li XH, Zhang W, Zhao WS, Yan JY 2016 – Notes on currently accepted species of Colletotrichum. Mycosphere 7(8) 1192–1260, Doi 10.5943/mycosphere/si/2c/9 Abstract Colletotrichum is an economically important plant pathogenic genus worldwide, but can also have endophytic or saprobic lifestyles. The genus has undergone numerous revisions in the past decades with the addition, typification and synonymy of many species. In this study, we provide an account of the 190 currently accepted species, one doubtful species and one excluded species that have molecular data. Species are listed alphabetically and annotated with their habit, host and geographic distribution, phylogenetic position, their sexual morphs and uses (if there are any known).
    [Show full text]
  • Colletotrichum: Biological Control, Bio- Catalyst, Secondary Metabolites and Toxins
    Mycosphere 7(8) 1164-1176(2016) www.mycosphere.org ISSN 2077 7019 Article Doi 10.5943/mycosphere/si/2c/7 Copyright © Guizhou Academy of Agricultural Sciences Mycosphere Essay 16: Colletotrichum: Biological control, bio- catalyst, secondary metabolites and toxins Jayawardena RS1,2, Li XH1, Liu M1, Zhang W1 and Yan JY1* 1 Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, People’s Republic of China 2 Center of Excellence in Fungal Research and School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand Jayawardena RS, Li XH, Liu M, Zhang W, Yan JY 2016 – Mycosphere Essay 16: Colletotrichum: Biological control, bio-catalyst, secondary metabolites and toxins. Mycosphere 7(8) 1164–1176, Doi 10.5943/mycosphere/si/2c/7 Abstract The genus Colletotrichum has received considerable attention in the past decade because of its role as an important plant pathogen. The importance of Colletotrichum with regard to industrial application has however, received little attention from scientists over many years. The aim of the present paper is to explore the importance of Colletotrichum species as bio-control agents and as a bio-catalyst as well as secondary metabolites and toxin producers. Often the names assigned to the above four industrial applications have lacked an accurate taxonomic basis and this needs consideration. The current paper provides detailed background of the above topics. Key words – biotransformation – colletotrichin – mycoherbicide – mycoparasites – pathogenisis – phytopathogen Introduction Colletotrichum was introduced by Corda (1831), and is a coelomycete belonging to the family Glomerellaceae (Maharachchikumbura et al. 2015, 2016). Species of this genus are widely known as pathogens of economical crops worldwide (Cannon et al.
    [Show full text]
  • Musa Species (Bananas and Plantains) Authors: Scot C
    August 2006 Species Profiles for Pacific Island Agroforestry ver. 2.2 www.traditionaltree.org Musa species (banana and plantain) Musaceae (banana family) aga‘ (ripe banana) (Chamorro), banana, dessert banana, plantain, cooking banana (English); chotda (Chamorro, Guam, Northern Marianas); fa‘i (Samoa); hopa (Tonga); leka, jaina (Fiji); mai‘a (Hawai‘i); maika, panama (New Zealand: Maori); meika, mei‘a (French Polynesia); siaine (introduced cultivars), hopa (native) (Tonga); sou (Solomon Islands); te banana (Kiribati); uchu (Chuuk); uht (Pohnpei); usr (Kosrae) Scot C. Nelson, Randy C. Ploetz, and Angela Kay Kepler IN BRIEF h C vit Distribution Native to the Indo-Malesian, E El Asian, and Australian tropics, banana and C. plantain are now found throughout the tropics and subtropics. photo: Size 2–9 m (6.6–30 ft) tall at maturity. Habitat Widely adapted, growing at eleva- tions of 0–920 m (0–3000 ft) or more, de- pending on latitude; mean annual tempera- tures of 26–30°C (79–86°F); annual rainfall of 2000 mm (80 in) or higher for commercial production. Vegetation Associated with a wide range of tropical lowland forest plants, as well as nu- merous cultivated tropical plants. Soils Grows in a wide range of soils, prefer- ably well drained. Growth rate Each stalk grows rapidly until flowering. Main agroforestry uses Crop shade, mulch, living fence. Main products Staple food, fodder, fiber. Yields Up to 40,000 kg of fruit per hectare (35,000 lb/ac) annually in commercial or- Banana and plantain are chards. traditionally found in Pacific Intercropping Traditionally grown in mixed island gardens such as here in Apia, Samoa, although seri- cropping systems throughout the Pacific.
    [Show full text]
  • A Worldwide List of Endophytic Fungi with Notes on Ecology and Diversity
    Mycosphere 10(1): 798–1079 (2019) www.mycosphere.org ISSN 2077 7019 Article Doi 10.5943/mycosphere/10/1/19 A worldwide list of endophytic fungi with notes on ecology and diversity Rashmi M, Kushveer JS and Sarma VV* Fungal Biotechnology Lab, Department of Biotechnology, School of Life Sciences, Pondicherry University, Kalapet, Pondicherry 605014, Puducherry, India Rashmi M, Kushveer JS, Sarma VV 2019 – A worldwide list of endophytic fungi with notes on ecology and diversity. Mycosphere 10(1), 798–1079, Doi 10.5943/mycosphere/10/1/19 Abstract Endophytic fungi are symptomless internal inhabits of plant tissues. They are implicated in the production of antibiotic and other compounds of therapeutic importance. Ecologically they provide several benefits to plants, including protection from plant pathogens. There have been numerous studies on the biodiversity and ecology of endophytic fungi. Some taxa dominate and occur frequently when compared to others due to adaptations or capabilities to produce different primary and secondary metabolites. It is therefore of interest to examine different fungal species and major taxonomic groups to which these fungi belong for bioactive compound production. In the present paper a list of endophytes based on the available literature is reported. More than 800 genera have been reported worldwide. Dominant genera are Alternaria, Aspergillus, Colletotrichum, Fusarium, Penicillium, and Phoma. Most endophyte studies have been on angiosperms followed by gymnosperms. Among the different substrates, leaf endophytes have been studied and analyzed in more detail when compared to other parts. Most investigations are from Asian countries such as China, India, European countries such as Germany, Spain and the UK in addition to major contributions from Brazil and the USA.
    [Show full text]
  • Monilochaetes and Allied Genera of the Glomerellales, and a Reconsideration of Families in the Microascales
    available online at www.studiesinmycology.org StudieS in Mycology 68: 163–191. 2011. doi:10.3114/sim.2011.68.07 Monilochaetes and allied genera of the Glomerellales, and a reconsideration of families in the Microascales M. Réblová1*, W. Gams2 and K.A. Seifert3 1Department of Taxonomy, Institute of Botany of the Academy of Sciences, CZ – 252 43 Průhonice, Czech Republic; 2Molenweg 15, 3743CK Baarn, The Netherlands; 3Biodiversity (Mycology and Botany), Agriculture and Agri-Food Canada, Ottawa, Ontario, K1A 0C6, Canada *Correspondence: Martina Réblová, [email protected] Abstract: We examined the phylogenetic relationships of two species that mimic Chaetosphaeria in teleomorph and anamorph morphologies, Chaetosphaeria tulasneorum with a Cylindrotrichum anamorph and Australiasca queenslandica with a Dischloridium anamorph. Four data sets were analysed: a) the internal transcribed spacer region including ITS1, 5.8S rDNA and ITS2 (ITS), b) nc28S (ncLSU) rDNA, c) nc18S (ncSSU) rDNA, and d) a combined data set of ncLSU-ncSSU-RPB2 (ribosomal polymerase B2). The traditional placement of Ch. tulasneorum in the Microascales based on ncLSU sequences is unsupported and Australiasca does not belong to the Chaetosphaeriaceae. Both holomorph species are nested within the Glomerellales. A new genus, Reticulascus, is introduced for Ch. tulasneorum with associated Cylindrotrichum anamorph; another species of Reticulascus and its anamorph in Cylindrotrichum are described as new. The taxonomic structure of the Glomerellales is clarified and the name is validly published. As delimited here, it includes three families, the Glomerellaceae and the newly described Australiascaceae and Reticulascaceae. Based on ITS and ncLSU rDNA sequence analyses, we confirm the synonymy of the anamorph generaDischloridium with Monilochaetes.
    [Show full text]
  • Advancing Banana and Plantain R & D in Asia and the Pacific
    AdvancingAdvancing bananabanana andand plantainplantain RR && DD inin AsiaAsia andand thethe PacificPacific -- VVol.ol. 1010 Proceedings of the 10th INIBAP-ASPNET Regional Advisory Committee meeting held at Bangkok, Thailand -- 10-11 November 2000 A.B. Molina, V.N. Roa and M.A.G. Maghuyop, editors The mission of the International Network for the Improvement of Banana and Plantain (INIBAP) is to sustainably increase the productivity of banana and plantain grown on smallholdings for domestic consumption and for local and export markets. The Programme has four specific objectives: • To organize and coordinate a global research effort on banana and plantain, aimed at the development, evaluation and dissemination of improved banana cultivars and at the conservation and use of Musa diversity. • To promote and strengthen collaboration and partnerships in banana-related activities at the national, regional and global levels. • To strengthen the ability of NARS to conduct research and development activities on bananas and plantains. • To coordinate, facilitate and support the production, collection and exchange of information and documentation related to banana and plantain. Since May 1994, INIBAP is a programme of the International Plant Genetic Resources Institute (IPGRI), a Future Harvest Centre. The International Plant Genetic Resources Institute (IPGRI) is an autonomous international scientific organization, supported by the Consultative Group on International Agricultural Research (CGIAR). IPGRI’s mandate is to advocate the conservation and use of plant genetic resources for the benefit of present and future generations. IPGRI’s headquarters is based in Rome, Italy, with offices in another 14 countries worldwide. It operates through three programmes: (1) the Plant Genetic Resources Programme, (2) the CGIAR Genetic Resources Support Programme, and (3) the International Network for the Improvement of Banana and Plantain (INIBAP).
    [Show full text]
  • (Musa Spp) in Malaysia
    Phenotypic and molecular characterization of Colletotrichum species associated with anthracnose of banana (Musa spp) in Malaysia M.A. Intan Sakinah, I.V. Suzianti and Z. Latiffah School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia Corresponding author: Z. Latiffah E-mail: [email protected] / [email protected] Genet. Mol. Res. 13 (2): 3627-3637 (2014) Received March 13, 2013 Accepted September 26, 2013 Published May 9, 2014 DOI http://dx.doi.org/10.4238/2014.May.9.5 ABSTRACT. Anthracnose caused by Colletotrichum species is a common postharvest disease of banana fruit. We investigated and identified Colletotrichum species associated with anthracnose in several local banana cultivars based on morphological characteristics and sequencing of ITS regions and of the β-tubulin gene. Thirty-eight Colletotrichum isolates were encountered in anthracnose lesions of five local banana cultivars, ‘berangan’, ‘mas’, ‘awak’, ‘rastali’, and ‘nangka’. Based on morphological characteristics, 32 isolates were identified as Colletotrichum gloeosporioides and 6 isolates as C. musae. C. gloeosporioides isolates were divided into two morphotypes, with differences in colony color, shape of the conidia and growth rate. Based on ITS regions and β-tubulin sequences, 35 of the isolates were identified as C. gloeosporioides and only 3 isolates as C. musae; the percentage of similarity from BLAST ranged from 95-100% for ITS regions and 97-100% for β-tubulin. C. gloeosporioides isolates were more prevalent compared to C. musae. This is the first record of C. gloeosporioides associated with banana anthracnose in Malaysia. In a phylogenetic analysis of the combined dataset of ITS regions and β-tubulin using a maximum likelihood method, C.
    [Show full text]
  • CARACTERIZAÇÃO DE ISOLADOS DE Colletotrichum Musae NO ESTADO DE PERNAMBUCO
    PAULO CÉZAR DAS MERCÊS SANTOS CARACTERIZAÇÃO DE ISOLADOS DE Colletotrichum musae NO ESTADO DE PERNAMBUCO RECIFE – PE FEVEREIRO – 2011 PAULO CÉZAR DAS MERCÊS SANTOS CARACTERIZAÇÃO DE ISOLADOS DE Colletotrichum musae NO ESTADO DE PERNAMBUCO Dissertação apresentada ao Programa de Pós- Graduação em Fitopatologia da Universidade Federal Rural de Pernambuco, como parte dos requisitos para obtenção do título de Mestre em Fitopatologia. RECIFE – PE FEVEREIRO – 2011 Ficha Catalográfica S237c Santos, Paulo Cézar das Mercês Caracterização de isolados de Colletotrichum musae no Estado de Pernambuco / Paulo Cézar das Mercês Santos. -- 2011. 97 f. : il. Orientador: Marcos Paz Saraiva Câmara. Dissertação (Mestrado em Fitopatologia) – Universidade Federal Rural de Pernambuco, Departamento de Agronomia, Recife, 2011. Referências. 1. Antracnose 2. Morfologia 3. Musa ssp. 4. Pós-colheita 5. Taxa de crescimento micelial 6. Testes bioquímicos I. Câmara, Marcos Paz Saraiva, Orientador II. Título CDD 582 CARACTERIZAÇÃO DE ISOLADOS DE Colletotrichum musae NO ESTADO DE PERNAMBUCO PAULO CÉZAR DAS MERCÊS SANTOS COMITÊ DE ORIENTAÇÃO: Prof. Ph. D. Marcos Paz Saraiva Câmara (UFRPE) – Orientador Prof. Dr. Sami Jorge Michereff (UFRPE) – Coorientador Prof. Dr. Ricardo Brainer Martins (UFAL) – Coorientador RECIFE – PE FEVEREIRO – 2011 CARACTERIZAÇÃO DE ISOLADOS DE Colletotrichum musae NO ESTADO DE PERNAMBUCO PAULO CÉZAR DAS MERCÊS SANTOS Dissertação defendida e aprovada pela Banca Examinadora em 17 de fevereiro de 2011. ORIENTADOR: ____________________________________________________ Prof. Ph. D. Marcos Paz Saraiva Câmara (UFRPE) EXAMINADORES: ____________________________________________________ Prof.ª Dr.ª Sônia Maria Alves de Oliveira (UFRPE) ____________________________________________________ Prof. Dr. Cristiano Souza Lima (UFRPE) ____________________________________________________ Pesq. Dr. Rildo Sartori Barbosa Coêlho (IPA) RECIFE – PE FEVEREIRO – 2011 A Deus, pelo dom da vida.
    [Show full text]
  • The Plant Pathogenic Genus Neocordana
    Plant Pathology & Quarantine 9(1): 139–151 (2019) ISSN 2229-2217 www.ppqjournal.org Article Doi 10.5943/ppq/9/1/12 The plant pathogenic genus Neocordana Samarakoon SMBC1,2, Wanasinghe DN3,5, Jeewon R4, Tian Q1,5, Jayawardena 1 2* RS and Chomnunti P 1 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand 2 School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand 3 World Agro Forestry Centre, East and Central Asia, 132 Lanhei Road, Kunming 650201, Yunnan, China 4 Department of Health Sciences, Faculty of Science, University of Mauritius, Reduit, Mauritius 5 Key Laboratory for Plant Biodiversity and Biogeography of East Asia (KLPB), Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, Yunnan, China Samarakoon SMBC, Wanasinghe DN, Jeewon R, Tian Q, Jayawardena RS, Chomnunti P 2019 – The plant pathogenic genus Neocordana. Plant Pathology & Quarantine 9(1), 139–151, Doi 10.5943/ppq/9/1/12 Abstract Neocordana species mainly causes leaf spots on Musa spp. (banana and plantain) and on Canna denudata (an ornamental plant). Leaf spots on Musa spp. reduce the quality of the commodity and result in significant economic loss. Based on molecular and morphological data, the genus accommodates seven species at present with a worldwide distribution. Most of the taxonomic work has been conducted on Neocordana musae. In this study, updates on the diversity, distribution, and morpho-molecular taxonomy of Neocordana are provided. In addition, important aspects such as pathogenicity, disease control, antagonistic activity and association with other pathogenic fungi are discussed. The phylogenetic relationships of Neocordana with other genera in the family Pyriculariaceae based on LSU and ITS DNA sequence data are investigated.
    [Show full text]
  • Morpho-Molecular Characterisation and Epitypification
    Fungal Diversity Morpho -molecular characterisation and epitypification of Colletotrichum capsici ( Glomerellaceae , Sordariomycetes ), the causative agent of anthracnose in chilli Belle Damodara Shenoy 1*, Rajesh Jeewon 2, Wing Hon Lam 2, Darbhe Jayarama Bhat 3, Po Po Than 4, P aul W.J. Taylor 5 and Kevin D. Hyde 2* 1Biodiversity (Mycology and Botany), Eastern Cereal and Oilseed Research Centre, 960 Carling Avenue, Ottawa, Ontario, K1A 0C6, Canada 2Centre for Research in Fungal Diversity, School of Biological Sciences, The Univers ity of Hong Kong, Pokfulam Road, Hong Kong SAR, PR China 3Department of Botany, Goa University, Goa - 403 206, India 4The Mushroom Research Cen tre , 128 Moo3 , Ba hn Pha Deng, T. Papae, A. Mae Taeng, Chiang Mai, 50150, Thailand 5BioMarka, School of Agricultur e and Food Systems, Faculty of Land and Food Resources, Corner of Royal Parade and Tin Alley, University of Melbourne, 3010, Victoria, Australia Shenoy, B.D., Jeewon, R., Lam, W.H., Bhat, D.J., Than, P.P. , Taylor, P.W.J. and Hyde, K.D. (2007). Morpho -mole cular characterisation and epitypification of Colletotrichum capsici (Glomerellaceae , Sordariomycetes ), the causative agent of anthracnose in chilli. Fungal Diversity 27: 197 -211 . Colletotrichum capsici is an economically important anamorphic taxon that c auses anthracnose in chilli ( Capsicum annuum , C . frutescens ). Vermicularia capsici (= Colletotrichum capsici ) deposited by H. Sydow in S was not designated as a holotype in the protologue and is, therefore, designated as the lectotype in this paper . T he sp ecimen is in relatively good condition, but could not provide viable cultures necessary to obtain DNA sequence data.
    [Show full text]