JOURNAL OF CLINICAL MICROBIOLOGY

Volume 45 September 2007 No. 9

MINIREVIEW

16S rRNA Gene Sequencing for Bacterial Identification in J. Michael Janda and Sharon L. 2761–2764 the Diagnostic Laboratory: Pluses, Perils, and Pitfalls Abbott

BACTERIOLOGY Is the Volume of Blood Cultured Still a Significant Factor in Emilio Bouza, Dolores Sousa, 2765–2769 the Diagnosis of ? Marta Rodrı´guez-Cre´ixems, Juan Garcı´a Lechuz, and Patricia Mun˜oz Reclassification of Phenotypically Identified Staphylococcus Takashi Sasaki, Ken Kikuchi, 2770–2778 intermedius Strains Yoshikazu Tanaka, Namiko Takahashi, Shinichi Kamata, and Keiichi Hiramatsu Evaluation of Gen-Probe APTIMA-Based Neisseria Erik Munson, Vivian Boyd, Jolanta 2793–2797 gonorrhoeae and Chlamydia trachomatis Confirmatory Testing Czarnecka, Judy Griep, Brian Lund, in a Metropolitan Setting of High Disease Prevalence Nancy Schaal, and Jeanne E. Hryciuk Convenient Test Using a Combination of Chelating Agents Soo-Young Kim, Seong Geun Hong, 2798–2801 for Detection of Metallo-␤-Lactamases in the Clinical Ellen S. Moland, and Kenneth S. Laboratory Thomson Molecular Characterization of Vancomycin-Resistant Bo Zheng, Haruyoshi Tomita, Yong 2813–2818 Enterococcus faecium Isolates from Mainland China Hong Xiao, Shan Wang, Yun Li, and Yasuyoshi Ike Bacteriology of Moderate-to-Severe Diabetic Foot Infections Diane M. Citron, Ellie J. C. 2819–2828 and In Vitro Activity of Antimicrobial Agents Goldstein, C. Vreni Merriam, Benjamin A. Lipsky, and Murray A. Abramson Outbreak of Infections with PER-1 Joanna Empel, Krzysztof Filczak, 2829–2834 Extended-Spectrum ␤-Lactamase in Warsaw, Poland: Agnieszka Mro´wka, Waleria Further Evidence for an International Clonal Complex Hryniewicz, David M. Livermore, and Marek Gniadkowski Multicenter Study To Evaluate Bloodstream Infection by Tetsuya Matsumoto, Mieko Goto, 2853–2857 cinaedi in Japan Hinako Murakami, Takashi Tanaka, Hiroyuki Nishiyama, Emi Ono, Chikako Okada, Etsuko Sawabe, Michiko Yagoshi, Akiko Yoneyama, Katsuko Okuzumi, Kazuhiro Tateda, Naoaki Misawa, and Keizo Yamaguchi Multicenter Evaluation of the BD Phoenix Automated Sandra S. Richter, Wanita J. 2863–2871 Microbiology System for Antimicrobial Susceptibility Howard, Melvin P. Weinstein, Testing of Streptococcus Species David A. Bruckner, Janet F. Hindler, Michael Saubolle, and Gary V. Doern Development of Real-Time PCR Assays and Evaluation of Chonthida Supaprom, Dongling 2894–2901 Their Potential Use for Rapid Detection of Burkholderia Wang, Chanvit Leelayuwat, pseudomallei in Clinical Blood Specimens Wisansanee Thaewpia, Wattanachai Susaengrat, Victor Koh, Eng Eong Ooi, Ganjana Lertmemongkolchai, and Yichun Liu

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Accuracy of Six Antimicrobial Susceptibility Methods for Fred C. Tenover, Portia P. Williams, 2917–2922 Testing Linezolid against Staphylococci and Enterococci Sheila Stocker, Angela Thompson, Leigh Ann Clark, Brandi Limbago, Roberta B. Carey, Susan M. Poppe, Dean Shinabarger, and John E. McGowan, Jr. Serotype IX, a Proposed New Streptococcus agalactiae Hans-Christian Slotved, Fanrong 2929–2936 Serotype Kong, Lotte Lambertsen, Susanne Sauer, and Gwendolyn L. Gilbert Vibrio vulnificus Typing Based on Simple Sequence Repeats: Yoav Y. Broza, Yael Danin-Poleg, 2951–2959 Insights into the Biotype 3 Group Larisa Lerner, Meir Broza, and Yechezkel Kashi Nasal Staphylococcus aureus Carriage Is Not a Risk Factor Sabine Ridder-Schaphorn, Felix 2979–2984 for Lower-Airway Infection in Young Cystic Fibrosis Ratjen, Angelika Du¨bbers, Johannes Patients Ha¨berle, Sabine Falk, Peter Ku¨ster, Antje Schuster, Uwe Mellies, Brigitte Lo¨we, Ralf Reintjes, Georg Peters, and Barbara C. Kahl DNA Pyrosequencing-Based Bacterial Ruth Ann Luna, Lea R. Fasciano, 2985–2992 Identification in a Pediatric Hospital Setting Shaunte C. Jones, Bobby L. Boyanton, Jr., Trang T. Ton, and James Versalovic The Binax NOW Test as a Tool for Diagnosis of Severe Marie Gisselsson-Sole´n, Anita 3003–3007 Acute Otitis Media and Associated Complications Bylander, Christina Wilhelmsson, Ann Hermansson, and Åsa Melhus Development of a Real-Time Fluorescence Resonance Khalil Abdelbaqi, Alice 3015–3021 Energy Transfer PCR To Detect Arcobacter Species Buissonnie`re, Vale´rie Prouzet- Mauleon, Jessica Gresser, Irene Wesley, Francis Me´graud, and Armelle Me´nard Methicillin-Resistant Staphylococcus aureus Infection or Clinton C. Haley, Deepa Mittal, 3031–3038 Colonization Present at Hospital Admission: Multivariable Amanda LaViolette, Sai Risk Factor Screening To Increase Efficiency of Surveillance Jannapureddy, Najma Parvez, and Culturing Robert W. Haley Use of Multiple-Displacement Amplification and L. C. N. Brito, F. R. Teles, R. P. 3039–3049 Checkerboard DNA-DNA Hybridization To Examine the Teles, E. C. Franc¸a, A. P. Ribeiro- Microbiota of Endodontic Infections Sobrinho, A. D. Haffajee, and S. S. Socransky Diversity of Mutations in the atpC Gene Coding for the c Cı´cero A. Dias, Grasiela Agnes, 3065–3067

Subunit of F0F1 ATPase in Clinical Isolates of Optochin- Ana Paula G. Frazzon, Filipe D. Resistant Streptococcus pneumoniae from Brazil Kruger, Pedro A. d’Azevedo, Maria da Glo´ria S. Carvalho, Richard R. Facklam, and Lu´cia M. Teixeira PCR-Based Identification of Common Colonization Factor Abhisek Ghosal, Rudra Bhowmick, 3068–3071 Antigens of Enterotoxigenic Ranjan Kumar Nandy, T. Ramamurthy, and Nabendu Sekhar Chatterjee Use of Quantitative PCR and Culture Methods To F. Dalwai, D. A. Spratt, and J. 3072–3076 Characterize Ecological Flux in Bacterial Biofilms Pratten Similarity of Bacterial Populations in Saliva from African- Yihong Li, Amid I. Ismail, Yao Ge, 3082–3085 American Mother-Child Dyads Marisol Tellez, and Woosung Sohn Development and Application of a Novel Peptide Nucleic N. Guimara˜es, N. F. Azevedo, C. 3089–3094 Acid Probe for the Specific Detection of in Figueiredo, C. W. Keevil, and M. J. Gastric Biopsy Specimens Vieira Evaluation of BBL CHROMagar O157 versus Sorbitol- D. L. Church, D. Emshey, H. 3098–3100 MacConkey Medium for Routine Detection of Escherichia Semeniuk, T. Lloyd, and J. D. coli O157 in a Centralized Regional Clinical Microbiology Pitout Laboratory Continued on following page Continued from preceding page

Revised Approach for Identification of Isolates within the Jane F. Turton, Nazia Arif, Daneeta 3105–3108 Burkholderia cepacia Complex and Description of Clinical Hennessy, Mary E. Kaufmann, and Isolates Not Assigned to Any of the Known Genomovars Tyrone L. Pitt

MYCOBACTERIOLOGY AND AEROBIC ACTINOMYCETES Development and Evaluation of a Line Probe Assay for Jun-Ichiro Sekiguchi, Tomohiko 2802–2807 Rapid Identification of pncA Mutations in Pyrazinamide- Nakamura, Tohru Miyoshi-Akiyama, Resistant Mycobacterium tuberculosis Strains Fumiko Kirikae, Intetsu Kobayashi, Ewa Augustynowicz-Kopec´, Zofia Zwolska, Koji Morita, Toshinori Suetake, Hiroshi Yoshida, Seiya Kato, Toru Mori, and Teruo Kirikae Characterization of Tunisian Mycobacterium tuberculosis Alya Soudani, Sondess Hadjfredj, 3095–3097 Rifampin-Resistant Clinical Isolates Me´riem Zribi, Afef Masmoudi, Taieb Messaoud, Hane´ne Tiouri, and Chedlia Fendri Implementation Validation Performed in Rwanda To Cindy Maria Quezada, Eliane 3111–3114 Determine Whether the INNO-LiPA Rif.TB Line Probe Kamanzi, Julienne Mukamutara, Assay Can Be Used for Detection of Multidrug-Resistant Pim De Rijk, Leen Rigouts, Mycobacterium tuberculosis in Low-Resource Countries Franc¸oise Portaels, and Yanis Ben Amor

MYCOLOGY Use of Bronchoalveolar Lavage To Detect Galactomannan M. Hong Nguyen, Reia Jaber, 2787–2792 for Diagnosis of Pulmonary Aspergillosis among Helen L. Leather, John R. Wingard, Nonimmunocompromised Hosts Benjamin Staley, L. Joseph Wheat, Christina L. Cline, Maher Baz, Kenneth H. Rand, and Cornelius J. Clancy Detection of Circulating Galactomannan in Serum Samples Yu-Tsung Huang, Chien-Ching 2858–2862 for Diagnosis of Penicillium marneffei Infection and Hung, Chun-Hsing Liao, Hsin-Yun Cryptococcosis among Patients Infected with Human Sun, Shan-Chwen Chang, and Yee- Immunodeficiency Virus Chun Chen Reverse Line Blot Hybridization Assay for Identification of Xianyu Zeng, Fanrong Kong, 2872–2880 Medically Important Fungi from Culture and Clinical Catriona Halliday, Sharon Chen, Specimens Anna Lau, Geoffrey Playford, and Tania C. Sorrell Detection of Fifteen Species of Candida in an Automated Lynn L. Horvath, Benjamin J. 3062–3064 Blood Culture System George, and Duane R. Hospenthal First Contemporary Case of Human Infection with Arlo Upton, James A. Fraser, Sarah 3086–3088 Cryptococcus gattii in Puget Sound: Evidence for Spread of E. Kidd, Camille Bretz, Karen H. the Vancouver Island Outbreak Bartlett, Joseph Heitman, and Kieren A. Marr

PARASITOLOGY Rapid Microsphere Assay for Identification of Kakali Bandyopadhyay, Kathryn L. 2835–2840 Cryptosporidium hominis and Cryptosporidium parvum in Kellar, Iaci Moura, Maria Cristina Stool and Environmental Samples Casaqui Carollo, Thaddeus K. Graczyk, Susan Slemenda, Stephanie P. Johnston, and Alexandre J. da Silva Microsporidian Infection Is Prevalent in Healthy People in Stephenson W. Nkinin, Tazoacha 2841–2846 Cameroon Asonganyi, Elizabeth S. Didier, and Edna S. Kaneshiro

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Real-Time PCR Assay for Rapid Detection and Analysis of Jessica Keen, Gabriella A. Farcas, 2889–2893 PfCRT Haplotypes of Chloroquine-Resistant Plasmodium Kathleen Zhong, Seychelle falciparum Isolates from India Yohanna, Michael W. Dunne, and Kevin C. Kain New Method for Simultaneous Species-Specific Donato Traversa, Raffaella Iorio, 2937–2942 Identification of Equine Strongyles (Nematoda, Strongylida) Thomas R. Klei, Vitaliy A. by Reverse Line Blot Hybridization Kharchenko, Jakub Gawor, Domenico Otranto, and Olivier

Assessment of Use of Microsatellite Polymorphism Analysis J. Knapp, J. M. Bart, M. L. 2943–2950 for Improving Spatial Distribution Tracking of Echinococcus Glowatzki, A. Ito, S. Gerard, S. multilocularis Maillard, R. Piarroux, and B. Gottstein Molecular Study of Microsporidiosis Due to Enterocytozoon Anne Espern, Florent Morio, 2999–3002 bieneusi and Encephalitozoon intestinalis among Human Michel Miegeville, Hachimou Illa, Immunodeficiency Virus-Infected Patients from Two Moustapha Abdoulaye, Vanina Geographical Areas: Niamey, Niger, and Hanoi, Vietnam Meyssonnier, Eric Adehossi, Anne Lejeune, Phung Dac Cam, Bernard Besse, and Franc¸oise Gay-Andrieu

VIROLOGY MultiCode-PLx System for Multiplexed Detection of Frederick S. Nolte, David J. 2779–2786 Seventeen Respiratory Viruses Marshall, Christopher Rasberry, Sabina Schievelbein, Grier G. Banks, Gregory A. Storch, Max Q. Arens, Richard S. Buller, and James R. Prudent Comparison of the Abbott 7000 and Bayer 340 Systems for Helene C. Highbarger, Zonghui Hu, 2808–2812 Measurement of Hepatitis C Virus Load Shyam Kottilil, Julia A. Metcalf, Michael A. Polis, M. B. Vasudevachari, H. Clifford Lane, and Robin L. Dewar Comparison of Four Methods Using Throat Swabs To Zhen Zhu, Wenbo Xu, Emily S. 2847–2852 Confirm Rubella Virus Infection Abernathy, Min-Hsin Chen, Qi Zheng, Tongzhan Wang, Zhenying Zhang, Congyong Li, Changyin Wang, Weikuan He, Shujie Zhou, and Joseph Icenogle Real-Time Reverse Transcription-PCR Assay for Detection Jennifer D. Boddicker, Paul A. 2902–2908 of Mumps Virus RNA in Clinical Specimens Rota, Trisha Kreman, Andrea Wangeman, Louis Lowe, Kimberly B. Hummel, Robert Thompson, William J. Bellini, Michael Pentella, and Lucy E. DesJardin Development of a Respiratory Virus Panel Test for J. Mahony, S. Chong, F. Merante, S. 2965–2970 Detection of Twenty Human Respiratory Viruses by Use of Yaghoubian, T. Sinha, C. Lisle, and Multiplex PCR and a Fluid Microbead-Based Assay R. Janeczko The Amino Acid Residues at Positions 120 to 123 Are Yongjun Tian, Yang Xu, Zhenhua 2971–2978 Crucial for the Antigenicity of Hepatitis B Surface Antigen Zhang, Zhongji Meng, Li Qin, Mengji Lu, and Dongliang Yang Hantavirus Disease Outbreak in Germany: Limitations of Stefan Schilling, Petra Emmerich, 3008–3014 Routine Serological Diagnostics and Clustering of Virus Boris Klempa, Brita Auste, Ebbo Sequences of Human and Rodent Origin Schnaith, Herbert Schmitz, Detlev H. Kru¨ger, Stephan Gu¨nther, and Helga Meisel Subgroup Prevalence and Genotype Circulation Patterns of Kalina T. Zlateva, Leen Vijgen, 3022–3030 Human Respiratory Syncytial Virus in Belgium during Ten Nathalie Dekeersmaeker, Cecilia Successive Epidemic Seasons Naranjo, and Marc Van Ranst

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Serotype 1 and 2 Bovine Noroviruses Are Endemic in Cattle S. L. Oliver, E. Wood, E. Asobayire, 3050–3052 in the United Kingdom and Germany D. C. Wathes, J. S. Brickell, M. Elschner, P. Otto, P. R. Lambden,

Impact of Hepatitis C Virus (HCV) Genotypes on Edouard Tuaillon, Anne-Marie 3077–3081 Quantification of HCV RNA in Serum by COBAS Mondain, Laure Ottomani, Laurent AmpliPrep/COBAS TaqMan HCV Test, Abbott Roudie`re, Pascal Perney, Marie- Antiretroviral Therapy HCV RealTime Assay, and Christine Picot, Fabienne Se´guret, VERSANT HCV RNA Assay Franc¸ois Blanc, Dominique Larrey, Philippe Van de Perre, and Jacques Ducos Impact of the COBAS AmpliPrep/COBAS AMPLICOR HIV- Jeffrey J. Germer, Jordan L. Bendel, 3101–3104 1 MONITOR Test, Version 1.5, on Clinical Laboratory Craig A. Dolenc, Sarah R. Nelson, Operations Amanda L. Masters, Tara M. Gerads, Jayawant N. Mandrekar, P. Shawn Mitchell, and Joseph D. C. Yao Testing of Diagnostic Methods for Detection of Influenza Mercedes Pe´rez-Ruiz, Ruth Yeste, 3109–3110 Virus for Optimal Performance in the Context of an Marı´a-Jose´ Ruiz-Pe´rez, Alfonso Influenza Surveillance Network Ruiz-Bravo, Manuel de la Rosa- Fraile, and Jose´ Marı´a Navarro- Marı´

CLINICAL VETERINARY MICROBIOLOGY Characterization of Aerococcus viridans Isolates from Swine V. Martı´n, A. I. Vela, M. Gilbert, J. 3053–3057 Clinical Specimens Cebolla, J. Goyache, L. Domı´nguez, and J. F. Ferna´ndez-Garayza´bal

EPIDEMIOLOGY Changes in the Clonal Nature and Resistance M. L. Amorim, N. A. Faria, D. C. 2881–2888 Profiles of Methicillin-Resistant Staphylococcus aureus Oliveira, C. Vasconcelos, J. C. Isolates Associated with Spread of the EMRSA-15 Clone in Cabeda, A. C. Mendes, E. Calado, a Tertiary Care Portuguese Hospital A. P. Castro, M. H. Ramos, J. M. Amorim, and H. de Lencastre Molecular Epidemiology and Distribution of Serotypes, Giovanni Gherardi, Monica Imperi, 2909–2916 Surface Proteins, and Antibiotic Resistance among Group B Lucilla Baldassarri, Marco Streptococci in Italy Pataracchia, Giovanna Alfarone, Simona Recchia, Graziella Orefici, Giordano Dicuonzo, and Roberta Creti Molecular Epidemiological and Antibiotic Susceptibility Cinzia Marianelli, Caterina 2923–2928 Characterization of Brucella Isolates from Humans in Sicily, Graziani, Carmela Santangelo, Italy Maria Teresa Xibilia, Alida Imbriani, Rosa Amato, Domenico Neri, Mario Cuccia, Sebastiano Rinnone, Vincenzo Di Marco, and Franco Ciuchini Deletion of the Meningococcal fetA Gene Used for Antigen Heike Claus, Johannes Elias, 2960–2964 Sequence Typing of Invasive and Commensal Isolates from Christine Meinhardt, Matthias Germany: Frequencies and Mechanisms Frosch, and Ulrich Vogel Performance of the BD GeneOhm Methicillin-Resistant Suzanne M. Paule, Donna M. 2993–2998 Staphylococcus aureus Test before and during High-Volume Hacek, Bridget Kufner, Karine Clinical Use Truchon, Richard B. Thomson, Jr., Karen L. Kaul, Ari Robicsek, and Lance R. Peterson Stability of Multiple-Locus Variable-Number Tandem K. L. Hopkins, C. Maguire, E. Best, 3058–3061 Repeats in Serovar Typhimurium E. Liebana, and E. J. Threlfall

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CASE REPORTS Isolation of Salmonella enterica Serotype Newport from a Dennis Tappe, Andreas Mu¨ller, 3115–3117 Partly Ruptured Splenic Abscess in a Traveler Returning Heinz-Jakob Langen, Matthias from Zanzibar Frosch, and August Stich Bacterial Peritonitis Caused by Jason J. Bofinger, Thomas Fekete, 3118–3120 and Rafik Samuel First Pulmonary Case Reported in Argentina of Infection M. Gutierrez, M. Feola, L. Lenge, 3121–3124 with Mycobacterium szulgai, a Rare Pathogen R. Rey, and M. Hoffman Prosthetic Valve Endocarditis Caused by Streptobacillus Po-Lin Chen, Nan-Yao Lee, Jing- 3125–3126 moniliformis: a Case of Rat Bite Fever Jou Yan, Yu-jen Yang, Hung-Mo Chen, Chia-Ming Chang, Hsin-Chun Lee, Nai-Ying Ko, Chao-Han Lai, and Wen-Chien Ko Outbreak of Mycobacterium massiliense Infection Associated Hee-Youn Kim, Yeo-Jun Yun, 3127–3130 with Intramuscular Injections Chan Geun Park, Dong Han Lee, Yong Kyun Cho, Byung Joo Park, Sae-Ick Joo, Eui-Chong Kim, Young Joo Hur, Bum-Joon Kim, and Yoon-Hoh Kook Molecular Typing of Beta-Hemolytic Streptococci from Two Ingibjo¨rg Hilmarsdo´ttir and Freyja 3131–3132 Patients with Lower-Limb : Identical Isolates from Valsdo´ttir Toe Web and Blood Specimens Molecular Diagnosis of Kingella kingae Pericarditis by Matta Matta, Delphine Wermert, 3133–3134 Amplification and Sequencing of the 16S rRNA Gene Isabelle Podglajen, Olivier Sanchez, Annie Buu-Hoı¨, Laurent Gutmann, Guy Meyer, and Jean-Luc Mainardi Community-Acquired Lung Abscess Caused by Legionella Louis P. Girard and Daniel B. 3135–3137 micdadei in a Myeloma Patient Receiving Thalidomide Gregson Treatment Severe Toxoplasma gondii I/III Recombinant-Genotype Se´verine Genot, Jacqueline Franck, 3138–3140 Encephalitis in a Human Immunodeficiency Virus Patient Jean-Marie Forel, Stanislas Rebaudet, Daniel Ajzenberg, Andre Maues de Paula, Marie-Laure Darde´, Andreas Stein, and Ste´phane Ranque

FAST-TRACK COMMUNICATION Intravenous PLASMA-LYTE as a Major Cause of False- Zdenek Racil, Iva Kocmanova, 3141–3142 Positive Results of Platelia Aspergillus Test for Martina Lengerova, Jana Winterova, Galactomannan Detection in Serum and Jiri Mayer

LETTERS TO THE EDITOR Culture-Independent Identification of the Source of an Paul Christian Lu¨ck, Christa Ecker, 3143–3144 Infection by Direct Amplification and Sequencing of Udo Reischl, Hans-Jo¨rg Linde, and DNA from a Clinical Specimen Roland Stempka Lactobacillus iners: a Marker of Changes in the Vaginal Tell Jakobsson and Urban Forsum 3145 Flora? Emergence of an Epidemic Clone of Community-Associated Letter: Silvia Campana, Priscilla 3146–3147 Methicillin-Resistant Panton-Valentine Leucocidin-Negative Cocchi, Gerd Do¨ring, and Giovanni Staphylococcus aureus in Cystic Fibrosis Patient Populations Taccetti. Reply: Shannon M. Moroney

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ERRATA Antibiotic-Resistant Invasive Pneumococcal Clones in Italy Giovanni Gherardi, Loredana 3148 Fallico, Maria Del Grosso, Federica Bonanni, Fabio D’Ambrosio, Riccardo Manganelli, Giorgio Palu`, Giordano Dicuonzo, and Annalisa Pantosti Spoligotype Signatures in the Mycobacterium tuberculosis E. M. Streicher, T. C. Victor, G. 3149 Complex van der Spuy, C. Sola, N. Rastogi, P. D. van Helden, and R. M. Warren Association between the Presence of the Panton-Valentine C. Lopez-Aguilar, E. Perez-Roth, 3150 Leukocidin-Encoding Gene and a Lower Rate of Survival among Hospitalized Pulmonary Patients with Staphylococcal Disease