Germination, Emergence, and Early Growth of Spartina Pectinata
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University of Northern Iowa UNI ScholarWorks Dissertations and Theses @ UNI Student Work 2001 Germination, emergence, and early growth of Spartina pectinata Wade Herold Williams University of Northern Iowa Let us know how access to this document benefits ouy Copyright ©2001 Wade Herold Williams Follow this and additional works at: https://scholarworks.uni.edu/etd Part of the Biology Commons Recommended Citation Williams, Wade Herold, "Germination, emergence, and early growth of Spartina pectinata" (2001). Dissertations and Theses @ UNI. 143. https://scholarworks.uni.edu/etd/143 This Open Access Thesis is brought to you for free and open access by the Student Work at UNI ScholarWorks. It has been accepted for inclusion in Dissertations and Theses @ UNI by an authorized administrator of UNI ScholarWorks. For more information, please contact [email protected]. GERMINATION, EMERGENCE, AND EARLY GROWTH OF SPARTINA PECTINATA An Abstract of a Thesis Submitted In Partial Fulfillment of the Requirements for the Degree Master of Science 10 Wade Herold Williams University ofNorthem Iowa May2001 ABSTRACT Spartina pectinata Link is frequently omitted from lowland prairie restorations because the seed often fails to grow when planted, and inclusion is limited to plugs of young plants. Anecdotal reports indicated improvement of seed germination following stratification. The effects of seed stratification on the germination of S. pectinata seed were systematically studied. Seed was obtained from both relict populations of plants and germplasm plants as defined by Englert and White (1977). Seed from 5 different collections were subjected to 4 different seed stratifying treatments: imbibed-chilled (1 oc for 15 to 45 days); imbibed-frozen (-12°C for 4 to 7 days); or dried-warmed (23 oc for 120 days); or aged (4°C for 1.5 to 2.5 years). Control (unstratified) seed for all trials was fresh, dried-chilled seed ofthe same collection, stored in a refrigerator at 4°C. In addition, seed from 4 collections planted in soil were subjected to 2 different seed stratifying methods (imbibed-chilled, imbibed-frozen) under greenhouse conditions. Emergence of germinating seedlings was monitored for post-emergence damping-off Also, seedlings from 2 collections were grown in the greenhouse and growth measurements were made weekly for 6 weeks after emergence from the soil. Control seed ranged in average viability from 66.5% (±7) to 99% (±1), in average germination from 59.5% (±7) to 94% (±2.2), and in average emergence from 38.5% (±3.8) to 84.5% (±6.8). The stratification treatments did not improve these values. Longer stratification periods at 1 oc for imbibed-chilled seed increased the germination percentage in the early phase of germination, this early increase was also reflected in coleoptile emergence from the soil. Total germination and emergence at the end of the trials were the same as the control. Stratifying seed by imbibed-freezing, dried-warming, or aging up to 1.5 years had no effect on its germination, though control seed germinated significantly better than 2.5-year old seed. Germination rates varied in collections from different sites. Post-emergence damping-off was not observed for any seed collection, but mildew became a problem for seed stratified by imbibed-chill beyond 45 days. For both relict and germplasm plants, the total length of leaves and roots steadily increased during the first 6 weeks. The majority of growth was below ground. Average seed depth decreased during early growth, rising several seed thicknesses from an average planting depth range of0.62 em (±0.11) to 0.69 em (±09). Endosperm starch levels steadily decreased through week 4 and were depleted by week 5. Six week-old seedlings had an average total shoot length range of30.40 em (±5.89) to 33 .32 em (±4.71) and an average total root length range of 139.40 (±39.40) em to 169.11 em (±52.42). It can be concluded from this study that Spartina pectinata should be included in prairie restoration projects, as viable seed will germinate readily with or without stratification. GERMJNATION, EMERGENCE, AND EARLY GROWTH OF SPARTINA PECTINATA A Thesis Submitted In Partial Fulfillment of the Requirements for the Degree Master of Science Wade Herold Williams University ofNorthem Iowa May 2001 11 This Study by: Wade Herold Williams Entitled: GERMINATION, EMERGENCE, AND EARLY GROWTH OF SPARTINA PECTINATA has been approved as meeting the thesis requirement for the Degree of Master of Science ~~/)/of Date Dr. Daryl D. Smith, Chair, Thesis Committee Am {A lv, Jto J I I Date ·J/?6/o 7 I Date !l!w~ ?~ 2co 1 Date ember tf ,bo(o f Date .. I -- - lll ACKNOWLEDGMENTS This master's thesis project couldn't have been completed without the help of many people. Thank you to: Wayne Lovelace (Forest Keeling Nursery) and Jerry Keiser (USDA-NRCS Plant Materials Center), Elsberry, MO who provided initial direction by sharing the methods and results of their tests on prairie cordgrass seed stratification; Dr. Susana Goggi and Elaine Roys (Iowa State University Seed Science Center, Ames) for instruction on seed germination; the faculty and staff of the Department of Biology, University ofNorthern Iowa, and members of my thesis committee for mentorship and equipment; Dr. Betty Zan (Department ofEducation, University ofNorthern Iowa) for use of her office computer and its advanced version of SAS; Greg Houseal (Iowa Ecotype Project, Cedar Falls) and Nancy Jensen and Wayne Duckwitz (USDA-NRCS Plant Materials Center, Bismarck, ND) for providing seed and technical assistance on a regular basis; Rich Wynia (USDA-NRCS Plant Materials Center, Manhattan, KS) and Dr. RH. Ries (USDA-ARS, Northern Great Plains Research Laboratory, Mandan, ND) for technical assistance; and the Anchor Paper Company, Minneapolis, MN for blotter paper. Funding was received from: the Iowa DOT Living Roadway Trust Fund; the Prem Sahai family, Webster City; the UNI Biology scholarship fund; the UNI College ofNatural Science GRASP grant program. Special thanks to Dr. Jean Gerrath, Amanda Dohrman, the Dave Williams family, Dr. Daryl Smith, and Abby Gulick for their help during the spring of2000. This project is respectfully dedicated to my family, whose patience, understanding, and support have allowed me to achieve this goaL ... lV TABLE OF CONTENTS PAGE LIST OF TABLES vii LIST OF FIGURES . x INTRODUCTION AND LITERATURE REVIEW . 1 Introduction . 1 Objectives . 3 Literature Review . 3 A Natural Resource . 3 Historic habitat and cultural use . 3 Extant distribution . 4 Taxonomy ............... ........ .............. 6 Wild vs. cultivated . 6 Flowering and Seed Set . 7 Reproductive morphology . 7 Seed Set . 8 Seed Handling . 9 Harvest . 9 Cleaning . 11 Storage . 11 Weight . 12 Germination and Growth . 12 Viability . 12 Stratifying nomenclature . 12 Breaking dormancy . 13 Germination . 15 Seedling Emergence . 16 Post Germination . 17 Insect Attack . : . 19 Statistical Treatment . 21 Nomenclature. .. 22 MATERIALS AND METHODS . 23 Preparation . 23 General... .... ...... ........... .. .......... ..... .. 23 Seed Origin and Collection . 24 Seed Treatments . 25 Germination Experiment Preparation . 27 Emergence Experiment Preparation . 27 v PAGE Imbibition and Chilling .................................. 28 Seed Testing .. .................................... .. ...... 28 Germination Trials ..... .... ................ ........... 28 Seed Viability . 29 Emergence Trials ...................................... 30 Growth ............. ... .................. ................ 32 Establishing Age Classes ................................. 32 Sampling ....... .... ... ............. .......... ... .. 32 IKI Test ............................................. 33 Growth Observations and Records ......................... 33 Growth Data Presentation . .. .... ..... ................. 34 Explanation ofMixed Models .. ... ......... ... ........ .... ... 34 RESULTS .......... ............................................ 41 Seed Production . 41 Seed Weight . .......... ..... ............ ... ........... 41 Germination . .......... .......................... .. ....... 42 Seed Viability . 42 Germination Reports . 42 Mixed Model Analysis ............. ... .................. 42 Germination Trial 1 . 48 Germination Trial 2 53 Germination Trial 3 57 Germination Trial 4 60 Germination Trial 5 63 Emergence ..... ....... ..... .. .. ... .... .. ....... .. ...... 63 Emergence Reports .................................... 63 Mixed Model Analysis .................................. 65 Emergence Trial1 ..................................... 66 Emergence Trial 2 ..................................... 72 Emergence Trial 3 . 75 Growth ........ ................. ... .......... ... ......... 78 Measurement of Growth ...................... ......... 78 Stunting ............................................. 88 DISCUSSION .................................................... 91 Germination . 92 Growth ................................................... 100 SUMMARY AND CONCLUSIONS ............. .. .... ............ .. 104 VI PAGE OTHER FACTORS . 106 LITERATURE CITED . 107 APPENDIX 1. Plant Material Classes . 116 APPENDIX 2. Recipe for 0.8% Tetrazolium Chloride Solution Containing 0.05 Molar Phosphate Buffer . 117 APPENDIX 3: SAS Mixed Linear Regression Models 1-5 .. 118 Vll LIST OF TABLES TABLE PAGE 1 Seed collections of S. pectinata used to measure germination, emergence and growth trials . 24 2 A summary of seed stratification methods used to test germination and emergence . 26 3 Seed weight