bioRxiv preprint doi: https://doi.org/10.1101/116533; this version posted March 13, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. MRCZ – A proposed fast compressed MRC file format and direct detector normalization strategies Robert A. McLeod1*, Ricardo Diogo Righetto1, Andy Stewart2, Henning Stahlberg1 1 Center for Cellular Imaging and NanoAnalytics (C-CINA), University of Basel, Basel, Switzerland 2 Department of Physics, University of Limerick, Limerick, Ireland * Corresponding author: Robert A. McLeod, C-CINA, Biozentrum, University of Basel (
[email protected]) Abstract The introduction of high-speed CMOS detectors is fast marching the field of transmission electron microscopy into an intersection with the computer science field of big data. Automated data pipelines to control the instrument and the initial processing steps are imposing more and more onerous requirements on data transfer and archiving. We present a proposal for expansion of the venerable MRC file format to combine integer decimation and lossless compression to reduce storage requirements and improve file read/write times by >1000 % compared to uncompressed floating-point data. The integer decimation of data necessitates application of the gain normalization and outlier pixel removal at the data destination, rather than the source. With direct electron detectors, the normalization step is typically provided by the vendor and is not open- source. We provide robustly tested normalization algorithms that perform at-least as well as vendor software.