Molecular Breeding 5: 511–520, 1999. 511 © 1999 Kluwer Academic Publishers. Printed in the Netherlands. A Sorghum propinquum BAC library, suitable for cloning genes associated with loss-of-function mutations during crop domestication Yann-Rong Lin1, Linghua Zhu1, Shuxin Ren1, Jinshui Yang2, Keith F. Schertz3 & Andrew H. Paterson1;4;∗ 1Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843-2474, USA; 2Institute of Genetics, Fudan University, Shanghai, 200433, P.R. China; 3USDA-ARS, College Station, TX 77843-2474, USA; 4Department of Crop and Soil Science, University of Georgia, Athens GA 30602, USA (∗author for correspondence, send correspondence to address #4; fax 002-1-706-583-0162; e-mail
[email protected]) Received 23 July 1998; accepted in revised form 23 March 1999 Key words: BAC, positional cloning, Sorghum propinquum Abstract AlargeinsertSorghum propinquum BAC library has been constructed to analyze the physical organization of the sorghum genome and to facilitate positional cloning of sorghum genes and QTLs associated with the early stages of grain crop domestication. This library was established from 12 different ligations using high-molecular-weight DNA generated from either one cycle or two cycles of size selection. This library consists of 38 016 BAC clones with an estimated average insert size of 126 kb and coverage of 6.6 genome equivalents. The 6.6 genome-equivalent BAC library of S. propinquum provides a 99.7% probability of finding one or more BACs that contain genes of interest. Twenty mapped DNA probes, ten homologous and ten heterologous, were used to screen the library, and 121 positive clones were identified, 6.05 per locus or 6.37 per probe.