TEST- LAB SKILLS 1. When Heating a Solution in a Test Tube, a Student Should A. Point the Test Tube in Any Direction C. Cork

TEST- LAB SKILLS

1. When heating a solution in a test tube, a student should

a. point the test tube in any direction c. cork the test tube

b. hold the test tube with two fingers d. wear goggles

2. To observe the aortic arches of an earthworm, a student should use

a. an ultracentrifuge c. a stereoscope

b. an electron microscope d. a pipette

3. What is the volume of the liquid indicated in the diagram below of a graduated cylinder?

a. 23ml b. 26ml c. 27ml d. 28ml

4. Which laboratory technique is illustrated in the diagram?

a. testing a specimen for amino acids

b. determining the pH of a specimen

c. measuring the photosynthetic rate in a specimen

d. preparing a wet mount of a specimen

5. The diameter of the field of view shown below is 1.25 millimeters.

The approximate diameter of each cheek cell is

a. 50 μm b. 150 μm c. 250 μm d. 350 μm

6. A student is investigating the internal parts of an earthworm. Which piece of equipment should the

student use to move the intestine aside without damaging it?

a. a glass slide b. a metric ruler c. a dissecting needle d. a dropping pipette

7. A cell in the field of view of a compound light microscope is shown in the diagram below. In which

direction should the slide be moved to center this cell in the microscopic field?

a. to the right and up c. to the left and up

b. to the right and down d. to the left and down

8. What is the approximate diameter of the cell shown in the low-power field of a compound light

microscope represented below?

a. 100 μm b. 500 μm c. 800 μm d. 1000 μm

9. A student observing a specimen using the low-power objective of a compound light microscope has

difficulty viewing the image because the field of view is too dark. The student can correct the

problem by

a. adjusting the diaphragm c. switching to the high-power objective

b. using the coarse adjustment d. cleaning the high-power objective

10. Which piece of laboratory equipment should a student use to remove the legs of a preserved

grasshopper for further study?

11. Which piece of equipment should be used to transfer a protist onto a microscope slide?

a. scissors b. dissecting needle c. medicine dropper d. forceps

12. While a student is heating a liquid in a test tube, the mouth of the tube should always be

a. corked with a rubber stopper c. allowed to cool off

b. pointed toward the student d. aimed away from everybody

Base your answers to questions 13 and 14 on the diagrams of the laboratory equipment below and on your knowledge of the living environment.

13. Which piece of laboratory equipment would be used to obtain the most accurate measure of the volume

of a glucose solution?

a. 1 b. 2 c. 3 d. 4

14. Which piece of equipment could be used to determine the mass of an earthworm?

a. 1 b. 2 c. 3 d. 4

15. Which paragraph describes the correct procedure for preparing a stained wet mount of onion epidermis?

a. Place a slice of onion epidermis on a slide. Add two drops of water and one drop of stain. Cover the slice by dropping a coverslip directly on top of it. Press on the coverslip to force air bubbles out. Add one drop of water to one edge of the coverslip and add one drop of stain to the opposite edge.

b. Add one drop of stain to a piece of onion epidermis. Using forceps, place the epidermis on a slide. Blot the epidermis with a piece of paper towel to remove the excess stain. Drop a coverslip onto the specimen.

c. Place a slice of onion epidermis on a slide. Add one drop of water. Put one edge of a coverslip in the water drop, then slowly lower the opposite edge to the water. Put one drop of stain at one edge of the coverslip. Put a piece of paper towel at the opposite side of the coverslip. Allow the towel to absorb some water so that the stain will move under the coverslip.

d. Add one drop of stain to a slide. Place a piece of onion epidermis on top of the stain. Use a piece of paper towel to absorb the stain. Drop a coverslip on the epidermis to flatten it out. Lift the coverslip and add a drop of water to the epidermis. Replace the coverslip.

16. The table below shows the position of slides of the letter “e” on the stages of four microscopes. The

image of the “e” as seen using each microscope is also shown.

Which letters correctly identify the microscopes most likely used to provide the information in the table?

a. A and D - compound light microscopes; B and C - dissecting microscopes

b. B and C - compound light microscopes; A and D - dissecting microscopes

c. C and D - compound light microscopes; A and B - dissecting microscopes

d. B and D - compound light microscopes; A and C - dissecting microscopes

17. A compound light microscope is represented in the diagram below.

Which microscope part is correctly paired with its function?

a. A – magnifies the image of the specimen

b. B – used for focusing only when the high-power objective is used

c. C – provides the field of view with the largest diameter

d. D – holds the specimen on the stage

18. Normal body temperature on the Fahrenheit temperature scale is 98.6°F. What is the body temperature

in degrees Celsius?

a. 20°C b. 37°C c. 40°C d. 98.6°C

19. A microscope is supplied with 10X and 15X eyepieces, and with 10X and 44X objectives. What is the

maximum magnification that can be obtained from this microscope?

a. 59X b. 150X c. 440X d. 660X

20. Of the following, which piece of laboratory equipment would be used to most accurately measure the

volume of a liquid?

a. beaker b. flask c. test tube d. graduated cylinder

21. Which diagram shows a correct measurement?

22. The diagram below represents a thermometer.

The temperature reading on this thermometer would most likely indicate the temperature

a. of the human body on a very hot summer day

b. at which water freezes

c. at which water boils

d. of a human with a very high fever

23. Which graduated cylinder represented below contains a volume of liquid closest to 15 milliliters?

24. Which would be the proper laboratory procedure to follow if some laboratory chemical splashed into a

student’s eyes?

a. Send someone to find the school nurse.

b. Rinse the eyes with water and do not tell the teacher because he or she might become upset.

c. Notify the teacher immediately, rinse the eyes with water, then ask farther advice.

d. Assume that the chemical is not harmful and no action is required.

Base your answers to questions 25 and 26 on the following diagram of a compound light microscope.

25. The part labeled 1 is used to

a. increase the amount of light reaching the specimen

b. focus with the high-power objective.

c. hold the lenses in place

d. focus with the low-power objective

26. To adjust the amount of light reaching the specimen, you would use the part labeled

a. 1 b. 2 c. 3 d. 4

27. A student performing an experiment noticed that the beaker of water being heated had a slight crack in

the glass, but was not leaking. What should the student do?

a. Discontinue heating and attempt to seal the crack.

b. Discontinue heating and report the defect to the instructor.

c. Discontinue heating and immediately take the beaker to the instructor.

d. Continue heating as long as fluid does not seep from the crack.

28. A protist measures 500 micrometers in diameter. What is the diameter of this organism in millimeters?

a. 0.5 b. 5.0 c. 50 d. 500

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