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provided by Elsevier - Publisher Connector Developmental Biology 283 (2005) 537 – 574 www.elsevier.com/locate/ydbio
Society for Developmental Biology 64th Annual Meeting
Hyatt Regency, San Francisco, CA
July 27–August 1, 2005
Organizing Committee: Judith Kimble (Chair, SDB President), Kathy Barton, Minx Fuller, Nipam Patel, Didier Stainier, Xin Sun, Bill Wood
Local Organizers: Didier Stainier, Ida Chow
Numbers in italics are program abstract numbers and names in bold are speakers. Poster assignments are listed at the end of the meeting program. Program
Wednesday, July 27 9 am–5 pm Satellite Symposia (not organized by SDB) Segmentation Seacliff AB Co-organizers: Kenro Kusumi and Olivier Pourquie´
Molecular Biology of Plant Development Seacliff CD Organizer: Kathy Barton
12–7 pm Meeting Registration Grand Foyer Poster Session I and Exhibits Set-up Pacific Concourse See poster assignments at the end of the program
7–9 pm President’s Symposium Grand Ballroom Fundamental Problems of Developmental Biology Chair: Judith Kimble, University of Wisconsin-Madison and HHMI, Madison, WI
7:00 The soma-germline dichotomy. G. Seydoux. Johns Hopkins University, Baltimore, MD
1 7:40 MicroRNAs and their regulatory roles in plants and animals. D.P. Bartel. MIT and Whitehead Institute for Biomedical Research, Cambridge, MA
8:20 The genetics and genomics of evolving new traits in vertebrates. D. Kingsley. Stanford University and HHMI, Stanford, CA
9–11 pm Opening Reception in Honor of Eric Olson, Pacific Concourse Developmental Biology Editor-in-Chief, for invaluable service to the community
Poster Session I and Exhibits Pacific Concourse See poster assignments at the end of the program
YDBIO-02007; No. of pages: 38; 4C: SDB Meeting Abstracts 539
Thursday, July 28
7:30 am–9 am Continental Breakfast at Posters and Funding Opportunities Session
8 am–5 pm Meeting Registration Grand Foyer
8–8:45 am Funding Opportunities in Developmental Biology Seacliff CD Moderator: Ida Chow, Society for Developmental Biology, Bethesda, MD Participants: Representatives of NSF, NIH, ACS, MOD and other funding agencies
9 am–12:25 pm Concurrent Symposia Session I Symposium 1: Chromatin, Transcription and Development Grand A Chair: Susan Strome, Indiana University, Bloomington, IN
2 9:00 Epigenetic control of the X chromosomes by the C. elegans MES proteins. S. Strome, L. Bender, J. Suh, C. Carroll, W. Wang, T. Phippen, R. Cao, Y. Zhang. Indiana University, Bloomington, IN; University of North Carolina, Chapel Hill, NC
9:30 Global roles for chromatin remodeling factors in gene expression and development. J. Tamkun. University of California-Santa Cruz, Santa Cruz, CA
3 10:00 Solving the signal-regulated enhancer: a structure–function approach. S. Barolo, P. Ference, C. Rogers, M. Subbarao, K. Golden. University of Michigan Medical School, Ann Arbor, MI
4 10:17 Lmp4 regulates Tbx5 transcriptional activity by controlling its nuclear localization. H-G. Simon, T. Camarata, A. Kulisz, T. Chew, B. Bimber, J. Yeung. Northwestern University, Chicago, IL
10:35 Coffee Break
5 10:50 Studies on an RNAi-like mechanism of germline DNA elimination in Tetrahymena thermophila. K. Mochizuki, M.A. Gorovsky. University of Rochester, Rochester, NY
11:20 An epigenetic switch essential for mammalian neural development. J. Crabtree. Stanford University, Stanford, CA
6 11:50 Geminin regulates neuronal differentiation by antagonizing Brg1 activity. K.L. Kroll, A. Herr, J. Lim, G.A. Richardson, H. Richardson, S. Seo. Washington University School of Medicine, St. Louis, MO; Peter MacCallum Cancer Centre, Victoria, Australia
7 12:07 Baf60c integrates Notch signaling at the mouse node to initiate left-right asymmetry and regulate node morphology. B.G. Bruneau, J.K. Takeuchi, H. Lickert, M. Yamamoto, H. Hamada, J. Rossant. Hospital for Sick Children, Samuel Lunenfeld Research Institute and University of Toronto, Toronto, Canada; Osaka University, CREST and Japan Science and Technology Corporation, Osaka, Japan
Symposium 2: Egg Polarity Grand BC Chair: Trudi Schupbach, Princeton University, Princeton, NJ
8 9:00 EGF receptor signaling and dorso-ventral axis establishment in Drosophila. T. Schupbach, J.S. Goodrich, K.N. Clouse, U. Abdu, M. Klovstad, A. Pane, K. Wehr. HHMI, Princeton University, Princeton, NJ; Ben Gurion University, Be’er Sheva, Israel
9 9:30 Regulation of cortical microfilament contractility in early Caenorhabditis elegans embryos. B. Bowerman, T. Kurz, J.H. Willis, D.R. Hamill. University of Oregon, Eugene, OR
10 10:00 Rotation of 1-cell C. elegans embryos inside the egg shell reveals an early step in establishment of left-right polarity and embryonic handedness. W.B. Wood, S. Schonegg. MPI-CBG, Dresden, Germany; University of Colorado, Boulder, CO 540 Developmental Biology Volume 283, 2005
11 10:17 Cilia driven fluid flow in Kupffer’s vesicle directs left-right patterning in zebrafish. J.D. Amack, J.J. Essner, H.J. Yost. University of Utah, Salt Lake City, UT
10:35 Coffee Break
12 10:50 Regulative development and acquiring spatial patterning in the early mouse embryo. M. Zernicka-Goetz. Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge, UK
13 11:20 Polarity of the mouse embryo is established at blastocyst and is not prepatterned. T. Hiiragi, N. Motosugi, T. Bauer, Z. Polanski, D. Solter. Max- Planck Institute, Freiburg, Germany
14 11:50 Regulation of egg and early embryo polarity of sea urchins by Disheveled. A. Wikramanayake, J. Bince, R. Xu, C. Peng, M. Hong, Y. Marikawa. University of Hawaii, Honolulu, HI
15 12:07 Hermes is a component of the Xcat2 RNP complex localized within Xenopus germ plasm. M.L. King, Y. Zhou, M. Kloc, P. Chang, E. Houliston, L. Etkin. University of Miami School of Medicine, Miami, FL; University of Texas M.D. Anderson Cancer Center, Houston, TX; CNRS/UPMC, Observatoire Oce´an- ologique, Villefranche sur mer, France
12:25 pm–3 pm Lunch on your own and Poster Session I See poster assignments at the end of the program
3–5 pm Concurrent Workshops Session I Workshop 1: Optics Technology Grand A Chair: John White, University of Wisconsin-Madison, Madison, WI
16 3:00 Application of fluorescence lifetime imaging to cell and developmental biology. J.G. White, D. Bird, K. Elicieri. University of Wisconsin-Madison, Madison, WI
3:25 The organization and function of the secretory pathway in the syncytial blastoderm of Drosophila. J. Lippincott-Schwartz. National Institutes of Health, Bethesda, MD
17 3:50 Multiphoton Fluorescence Lifetime Imaging Microscopy (FLIM) using a streak camera. B. Herman, K. Ramanujan, V. Centonze, M. Lopez-Cruzan. University of Texas Health Science Center, San Antonio, TX
18 4:15 Nonlinear optics and multidimensional imaging of developing tissues. W.A. Mohler, S.V. Plotnikov, A.B. Isaacson, A.C. Millard, P.J. Campagnola, V. Juneja. University of Connecticut Health Center, Farmington, CT
4:40 Discussion
Workshop 2: Road to the Professoriate: getting a job and writing a dynamite grant Grand BC Chair: Karen Bennett, University of Missouri, Columbia, MO
19 3:00 The Road to the Professorate: getting a job and writing a dynamite grant. K.L. Bennett. University of Missouri, Columbia, MO
3:05 How to get an NIH R01 grant. K. Yamamoto. University of California-San Francisco, San Francisco, CA
3:25 Getting a job: the view from the search committee. C. Wylie. Cincinnati Children’s Hospital Medical Center, Cincinnati, OH
20 3:45 Job interviews: handling the stress and finding a good fit. D. Drummond-Barbosa. Vanderbilt University Medical Center, Nashville, TN
4:05 Getting your first grant: a junior faculty perspective. P. Newmark. University of Illinois at Urbana-Champaign, Urbana, IL
4:25 Discussion SDB Meeting Abstracts 541
5–7 pm Dinner on your own
7–9 pm Plenary Session I: Cell in Development and Heredity Grand Ballroom Chair: Julie Ahringer, Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK
21 7:00 Genome-wide RNAi screening for the study of cell polarity in C. elegans. J. Ahringer. Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK
22 7:30 Generation of temporal identity in the Drosophila CNS. C. Doe, B. Pearson, R. Grosskortenhaus, A. Marusich, M. Cleary. University of Oregon/HHMI, Eugene, OR
23 8:00 Cilia and Hedgehog signaling in the mouse. D. Huangfu, T. Caspary, K. Anderson. Sloan-Kettering Institute, New York, NY
8:30 Transcriptional circuits of heart development. E. Olson. University of Texas Southwestern Medical Center, Dallas, TX
9–11 pm Poster Session I and Exhibits Pacific Concourse See poster assignments at the end of the program
Friday, July 29
7:30 am–9 am Continental Breakfast at Posters and SDB-AXXS Workshop
7:30 am–8:45 am SDB-AXXS Workshop Seacliff CD Career Options: Networking with SDB Resources Co-chairs: Yolanda Cruz, Oberlin College, Oberlin, OH and W. Sue Shafer, AXXS
8 am–5 pm Meeting Registration Grand Foyer
9 am–12:25 pm Concurrent Symposia II Symposium 3: Signaling: From Individual Molecules to Networks Grand A Chair: Marc Vidal, Harvard Medical School, Dana Farber Cancer Institute, Boston, MA
24 9:00 Interactome networks. M. Vidal. Harvard Medical School, Dana-Farber Cancer Institute, Boston, MA
25 9:30 Signaling and transcriptional network controlling patterns of cell type in C. elegans organogenesis. P.W. Sternberg, T. Inoue, B.J. Hwang, J.S. Fernandes, T.O. Ririe, J. Sanders, D. Sherwood, A. Seah. HHMI and California Institute of Technology, Pasadena, CA
26 10:00 The impact of cell geometry on planar cell polarity. D. Ma, K. Amonlirdviman, C.J. Tomlin, J.D. Axelrod. Stanford University, Stanford, CA
27 10:17 Endocytic control of cell signalling and epithelial polarity by the Drosophila syntaxin avalanche. D. Bilder, H. Lu. University of California-Berkeley, Berkeley, CA
10:34 Coffee Break
10:50 The gene network controlling gastrulation of Drosophila embryos. A. Stathopou- los. California Institute of Technology, Pasadena, CA
28 11:20 Probing Wnt signaling using small molecule regulation of protein stability and localization. K.J. Liu, J.R. Arron, J.E. Gestwicki, K. Stankunas, J. Bayle, V. Devasthali, J.C. Baker, G.R. Crabtree. Stanford University Medical Center/HHMI, Stanford, CA 542 Developmental Biology Volume 283, 2005
29 11:37 A Pbx1-dependent genetic and transcriptional network regulates spleen ontogeny. L. Selleri, A. Brendolan. Cornell University Medical School, New York, NY
30 11:54 Exploring Smad signaling pathways in normal and cancer cells. X. Liu,S.Zhu, T. Cheung, R. Erickson, W. Wang, D. Clarke. University of Colorado-Boulder, Boulder, CO Symposium 4: Stem Cells and Regeneration Grand BC Chair: Kathy Barton, Carnegie Institution of Washington, Stanford, CA
31 9:00 Polarity and proliferation in the Arabidopsis meristem. K. Barton. Carnegie Institution of Washington, Stanford, CA
32 9:30 Stem cell and regeneration regulators in the planarian Schmidtea mediterranea. A. Sa´nchez Alvarado, P.W. Reddien, A. Bermange, K. Murfitt, J.R. Jennings. University of Utah School of Medicine, Salt Lake City, UT; London Research Institute, London, United Kingdom
33 10:00 Fgf-20 is required for blastema formation in zebrafish caudal fin regeneration. G.G. Whitehead, S. Makino, C. Lien, S. Kim, P. Jwahar, M.T. Keating. HHMI, Harvard Medical School Children’s Hospital, Boston, MA
34 10:17 Stem cells for the anterior pituitary identified by Nestin-GFP transgene expression. A.S. Gleiberman, J.M. Encinas, J.L. Roig, T. Michurina, P. Krasnov, M.G. Rosenfeld, G. Enikolopov. University of California-San Diego, San Diego, CA; Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
10:34 Coffee Break
10:50 Regulation of stem cell self renewal and differentiation by the support cell niche. M. Fuller. Stanford University, Stanford, CA
35 11:20 Neural crest stem cell depletion mediated by loss of Tcof1 causes Treacher Collins Syndrome craniofacial abnormalities. P.A. Trainor, N.C. Jones, J. Dixon, M. Dixon. Stowers Institute for Medical Research, Kansas City, MO; University of Manchester, United Kingdom
36 11:37 Roles for Myc and the cell cycle machinery in self-renewal of murine ES cells. S. Dalton, M. Bechard, C. McLean. University of Georgia, Athens, GA
37 11:54 Stem cell self-renewal and aging reflect a balance between repression and age- related activation of the INK4A and ARF senescence pathways. R. Pardal, A.V. Molofsky, S. He, G.M. Kruger, S.J. Morrison. HHMI, University of Michigan, Ann Arbor, MI
12:25 pm–3 pm Lunch on your own and Poster Session I See poster assignments at the end of the program Poster Session I tear down and Poster Session II set-up at the end of the session
2:–2:30 pm SDB Business Meeting Grand Ballroom
3–5 pm Plenary Workshop: Concepts in Developmental Biology: What Are They? Grand Ballroom Chair: Bill Wood, University of Colorado-Boulder, Boulder, CO Discussants: Bill Wood. University of Colorado-Boulder, Boulder, CO Scott Gilbert. Swarthmore College, Swarthmore, PA Judith Kimble. University of Wisconsin-Madison and HHMI, Madison, WI Marc Kirschner. Harvard Medical School, Boston, MA
5–6 pm Meet the SDB Directors Reception for Students and Postdocs Garden Room
5–7 pm Dinner on your own
7–9 pm Plenary Session: Awards Lectures Grand Ballroom Chair: Elliot Meyerowitz, California Institute of Technology, Pasadena, CA
E.G. Conklin Medal 7:00 Award presentation to Nicole M. LeDouarin by Elliot Meyerowitz, SDB President-elect SDB Meeting Abstracts 543
38 7:05 The neural crest and the development of the vertebrate head. N.M. Le Douarin. Inst d’Embryol Cell et Mole´c du CNRS et Coll de France, France
Developmental Biology-SDB Lifetime Achievement Award 7:40 Award presentation to Ian Sussex by Judith Kimble, SDB President 39 7:45 Turning over a new leaf. I. Sussex. Yale University, New Haven, CT
Viktor Hamburger Outstanding Educator Prize 8:20 Award presentation to Bruce Alberts by Bill Wood, SDB Professional Development and Education Committee Chair
Saturday, July 30 7:30 am–9 am Continental Breakfast at Posters and Technical Roundtable
7:30 am–8:45 am Morpholino Technical Roundtable Seacliff CD Moderator: Jon Moulton, Gene Tools, LLC
8 am–5 pm Meeting Registration Grand Foyer
9 am–12:25 pm Concurrent Symposia III Symposium 5: Gradients in Space and Time Grand A Chair: Eric Wieschaus, Princeton University, Princeton, NJ
41 9:00 Morphogen gradients and scaling in insect embryos. E. Wieschaus, T. Gregor, A. McGregor, W. Bialek, D. Tank. HHMI, Princeton University, Princeton, NJ
9:30 Morphogen decoding Fon the fly_. Naama Barkai. Weizmann Institute of Science, Rehovot, Israel
42 10:00 Spatial bistability of Dpp-receptor interactions during Drosophila dorsal-ventral patterning. C. Ferguson, Y. Wang. University of Chicago, Chicago, IL
43 10:17 The role of BMPs in commissural axon guidance. S.J. Butler, K.D. Phan, J. Dodd. University of Southern California, Los Angeles, CA; Columbia University, New York, NY
10:34 Coffee Break
44 10:50 Whole genome analysis of foregut development in C. elegans. S.E. Mango. University of Utah, Salt Lake City, UT
45 11:20 A FOG-1/CPEB gradient controls the C. elegans germ line. B.E. Thompson, D.S. Bernstein, J.L. Bachorik, A.G. Petcherski, M. Wickens, J. Kimble. University of Wisconsin-Madison and HHMI, Madison, WI
46 11:37 Dissecting the roles of RA as a morphogen in the zebrafish hindbrain. R.J. White, T.F. Schilling. University of California-Irvine, Irvine, CA
11:54 Nodal signaling. A. Schier. Skirball Institute, New York University, New York, NY
Symposium 6: Metazoan Evolution Grand BC Chair: Sean Carroll, University of Wisconsin-Madison and HHMI, Madison, WI
47 9:00 Chance caught on the wing: Cis regulatory evolution and the origins of novelty. S.B. Carroll, N. Gompel, B. Prud’homme, T. Wittkopp, V. Kassner. University of Wisconsin-Madison and HHMI, Madison, WI; University of Cambridge, United Kingdom; Cornell University, Ithaca, NY
9:30 Heart migration in the Ciona tadpole. M. Levine. University of California-San Francisco, San Francisco, CA
48 10:00 Partial redundancy of regulatory elements of duplicated hoxb5 genes in teleosts. O. Jarinova, C. Prudhomme, L. Joly, L. Jeannotte, M. Ekker. University of Ottawa, Ottawa, Canada; University Laval, Quebec, Canada
49 10:17 Diverse segmental functions of pair-rule gene orthologs in Tribolium. C. Choe, S.J. Brown. Kansas State University, Manhattan, KS
10:34 Coffee Break 544 Developmental Biology Volume 283, 2005
50 10:50 Building divergent body plans with similar genetic pathways. B.J. Swalla. Friday Harbor Laboratories and University of Washington, Seattle, WA
51 11:20 Bat wing interdigit webbing is maintained by BMP attenuation and unique domains of FGF signaling. S.D. Weatherbee, C.J. Cretekos, R.R. Behringer, J.J. Rasweiler IV,L.A. Niswander. MSKCC, New York, NY; M.D. Anderson Cancer Center, Houston, TX; SUNY Downstate Medical Center, Brooklyn, NY; University of Colorado Health Science Center, Aurora, CO
52 11:37 The development of flight in Chiroptera: The morphologic and genetic evolution of bat wing digits. K.E. Sears, R.R. Behringer, L.A. Niswander. University of Colorado Health Science Center, Aurora, CO; University of Texas M.D. Anderson Cancer Center, Houston, TX
53 11:54 Deciphering the instructions for building a Hydra. R. Steele. University of California- Irvine, Irvine, CA
12:25 pm–3 pm Lunch on your own and Poster Session II See poster assignments at the end of the program
3–5 pm Concurrent Workshops Session II Workshop 3: Omics Technology Grand A Chair: Michael Sussman, University of Wisconsin-Madison, Madison, WI
54 3:00 Vive la difference: Profiling changes in the proteome and transcriptome of Arabidopsis caused by genetics and pharmacology. M.R. Sussman. University of Wisconsin-Madison, Madison, WI
3:25 Quantitative proteomic analysis of mammalian tissues. C. Wu. University of Colorado School of Medicine, Aurora, CO
55 3:50 Systems level analysis of the aging process. S.K. Kim. Stanford University, Stanford, CA
56 4:15 Mapping the genome’s second code. B. Ren. Ludwig Institute for Cancer Research and University of California-San Diego School of Medicine, La Jolla, CA
4:40 Discussion
Workshop 4: Developmental Biology 2010 Grand BC Chair: Robert DeHaan, Emory University, Atlanta, GA
57 3:00 Why should undergraduates be engaged in research? R.L. DeHaan. Emory University, Atlanta, GA
58 3:10 An education group experiment: Inspiration and engagement of students. G.C. Walker. MIT, Cambridge, MA
59 3:30 Undergraduate research experiences and the epigenesis of a science career. D.E. Lopatto. Grinnell College, Grinnell, IA
60 3:50 Developmental biology: how to engage engineering students. K.S. Anseth. HHMI and University of Colorado-Boulder, Boulder, CO
61 4:10 Mentoring undergraduate research projects. G.F. Hatfull. University of Pittsburgh, Pittsburgh, PA
62 4:30 Computer modeling of cells and early development: getting students involved. G.M. Odell. Friday Harbor Laboratory and University of Washington, WA
4:50 Discussion SDB Meeting Abstracts 545
5–7 pm Dinner on your own 7–9 pm Plenary Session II: On Growth and Form Grand Ballroom Chair: Bruce Edgar, Fred Hutchinson Cancer Research Center, Seattle, WA
63 7:00 Controlling cell proliferation during Drosophila wing development. B.A. Edgar, L. Buttitta, L. Li, H. Jiang, D. O’Keefe, D. Nickerson, O. Wartlick, A.A. de la Cruz. Fred Hutchinson Cancer Research Center, Seattle, WA
64 7:30 Dynamic genetics: A new view of plant growth. E.M. Meyerowitz, V. Reddy, M. Heisler, H. Jonsson, B. Shapiro, E. Mjolsness. California Institute of Technology, Pasadena, CA; Lund University, Lund, Sweden; University of California-Irvine, Irvine, CA
8:00 Cell migration in the zebrafish. C. Nu¨sslein-Volhard. Max-Planck-Institut fu¨r Entwicklungsbiologie, Tu¨bingen, Germany
8:30 Branching subroutines in mouse lung development. M. Krasnow. Stanford University/HHMI, Stanford, CA
9–11 pm Poster Session II Pacific Concourse
Sunday, July 31
7:30 am–9 am Continental Breakfast at Posters
8 am–5 pm Meeting Registration Grand Foyer
9 am–12:25 pm Concurrent Symposia IV Symposium 7: RNA Regulation and Development Grand A Chair: Ruth Lehmann, Skirball Institute/New York University, New York, NY
65 9:00 MicroRNAs controlling neuronal development. O. Hobert. Columbia University Medical Center, New York, NY
66 9:30 The plant exosome: role in embryo/endosperm identity choice and imprinting, functional specialization of its subunits, and novel RNA substrates. D.A. Belostotsky, S.V. Reverdatto, J.A. Chekanova, N.P. Skiba, J.M. Alonso, V.B. Brukhin, J.R. Ecker, U. Grossniklaus. State University of New York, Albany, NY; Harvard Medical School, Boston, MA; North Carolina State University, Raleigh, NC; University of Zurich, Switzerland; Salk Institute, La Jolla, CA
67 9:47 An RNA–protein complex that is required for efficient TGF-a secretion in Drosophila. J.E. Wilhelm, S. Sayles. Carnegie Institution of Washington, Baltimore, MD
68 10:04 To find, modify and control the messenger RNA: new PAPs, PUFs, and 3VUTRs. M. Wickens, L. Rouhana, J. Kwak, L. Opperman, N. Buter, C. Landry. University of Wisconsin-Madison, Madison, WI
10:34 Coffee Break
69 10:50 RNA recognition by the STAR proteins GLD-1 and Quaking. J.R. Williamson, S.P. Ryder, L.A. Frater, E.B. Goodwin. Scripps Research Institute, La Jolla, CA; University of Wisconsin-Madison, Madison, WI
70 11:20 miR-196 acts upstream of Hoxb-8 and Shh in limb development. E. Hornstein, J.H. Mansfield, S. Yekta, B.D. Harfe, M. McManus, S. Baskerville, D.P. Bartel, C.J. Tabin. Harvard Medical School, Boston, MA; Whitehead Institute for Biomedical Research, Cambridge, MA; University of Florida, Gainesville, FL; University of California-San Francisco, San Francisco, CA
71 11:37 A new mechanism for translational repression: tethering of caudal mRNA 5V and 3V ends by Bicoid and 4EHP. F. Poulin, P.F. Cho, Y.A. Cho-Park, I.B. Cho-Park, J.D. Chicoine, P. Lasko, N. Sonenberg. McGill University, Montre´al, Canada
11:54 Germ plasm architecture. R. Lehmann. Skirball Institute/New York University, New York, NY 546 Developmental Biology Volume 283, 2005
Symposium 8: Vertebrate Organogenesis Grand BC Chair: Didier Stainier, University of California-San Francisco, San Francisco, CA
9:00 Liver and pancreas development in zebrafish. D. Stainier. University of California- San Francisco, San Francisco, CA
72 9:30 The genetic mechanisms underlying brain ventricle morphogenesis in zebrafish. L.A. Lowery, H. Sive. Whitehead Institute/MIT, Cambridge, MA
73 9:47 Zebrafish from beyond establishes left-right asymmetry of the zebrafish brain. J.T. Gamse, K. Santhakumar, C. Thisse, B. Thisse, M.E. Halpern. Vanderbilt University, Nashville, TN; Carnegie Institution of Washington, Baltimore, MD; IGBMC, France
74 10:04 Patterning the endoderm into organ progenitor domains. K.S. Zaret, A. Calmont, K. Tremblay, G.R. Martin, R. Bort. Fox Chase Cancer Center, Philadelphia, PA; University of California-San Francisco, San Francisco, CA
10:34 Coffee Break
75 10:50 Genetic analysis of Sprouty gene function in the developing mouse embryo. G.R. Martin, G. Minowada, B. Yu, O. Klein, K. Shim. University of California-San Francisco, San Francisco, CA
76 11:20 Conditional inactivation of Fgfr1 in mouse defines its role in limb bud establishment, outgrowth and digit patterning. X. Sun, M. Lewandoski, C. Deng, B. Harfe, J. Verheyden. University of Wisconsin-Madison, Madison, WI; National Cancer Institute, Frederick, MD; NIDDK, National Institutes of Health, Bethesda, MD; University of Florida, Gainesville, FL
77 11:37 Regulation of branching morphogenesis in the mammary gland by MMP and FGF signaling. A.J. Ewald, P. Lu, J. Trumbull, G. Martin, Z. Werb. University of California-San Francisco, San Francisco, CA
11:54 Branch, bud and stem: the mouse lung as a model system for organogenesis. B. Hogan. Duke University, Durham, NC
12:25 pm–3 pm Lunch on your own and Poster Session II Pacific Concourse See poster assignments at the end of the program Posters and exhibits tear down at the end of the session
3–5 pm Plenary Session III: Ontogeny and Phylogeny Grand Ballroom Chair: Nipam Patel, University of California-Berkeley, Berkeley, CA
3:00 The evolution of arthropod segmentation and body patterning. N. Patel. University of California-Berkeley, Berkeley, CA
78 3:30 Shoots and leaves, an evo-devo perspective. N.R. Sinha,H.M.Garces,C.E.Champagne, K. Chung, S.J. Seiki, B.T. Townsley. University of California-Davis, Davis, CA; Univ. de Lisboa, Portugal
79 4:00 Gene-regulatory interactions in neural crest evolution and development. M. Bronner-Fraser. California Institute of Technology, Pasadena, CA
80 4:30 Hemichordates and the origin of chordates. J. Gerhart, C. Lowe, M. Kirschner. University of California-Berkeley, Berkeley, CA; University of Chicago, Chicago, IL; Harvard Medical School, Boston, MA
6:30 pm–7 pm Awards Reception Grand Foyer
7 pm–10 pm Awards Banquet and Awards Presentation Grand Ballroom Winners of Student Best Poster Competition will be announced SDB Meeting Abstracts 547
POSTER SESSIONS ABSTRACTS Poster Session I Pacific Concourse Wednesday, July 27: noon–5 pm (set-up), 9–11 pm Thursday, July 28: 12:30–3 pm, 9–11 pm Friday, July 29: 12:30–3 pm (tear-down at end of session) Numbers in italics indicate Program Abstract numbers. ‘‘B’’ numbers indicate Poster Board numbers. Odd number board authors present on Wednesday, July 27, 9–11 pm Even number board authors present on Thursday, July 28, 9–11 pm
Education
81 B1 Pipe cleaners and beads: Modeling molecular interactions involved in anterior–posterior pattern formation in Drosophila. K.R. Douglas. Augustana College, Rock Island, IL 61201
82 B2 Molecular lab activities for undergraduate developmental biology courses: Examining the expression of spicule matrix protein 30 (SM30) in Lytechinus variegatus embryos using RT-PCR. D.D. Ricker, J.P. Thompson. York College of Pennsylvania, York, PA 17405
83 B3 Molecular lab activities for undergraduate developmental biology courses: Examining the expression of TIE-2 protein and mRNA during zebrafish (Danio rerio) embryo development using ELISA and RT-PCR. D.D. Ricker, J.P. Thompson. York College of Pennsylvania, York, PA 17405
84 B4 Engaging undergraduates in a lab course using human adult stem cells and osteoblasts: Approaches and assessments. J.S. Doctor, K.M. Gallagher. Duquesne University, Pittsburgh, PA 15282
85 B5 The whole developmental class does one ten-week research project. J.E. Heady. University of Michigan-Dearborn, Dearborn, MI 48128
86 B6 Integration of research projects in standard undergraduate courses as a strategy to teach analytical skills and increase faculty research productivity. B.J. Avery, B.K. Baxter. Dept. of Biology, Westminster College, Salt Lake City, UT 84105, USA
87 B7 Ernest Everett Just (1883–1941)—An early ecological developmental biologist. W. Byrnes, W.R. Eckberg. Biochemistry and Molecular Biology and Biology Departments, Howard University, Washington, DC
Cell–Cell Signaling
88 B8 Spatio-temporal manipulation of signaling pathways at Rosa26 locus. J. Mao, J. Barrow, J. McMahon, J. Vaughan, A.P. McMahon. Harvard University, Cambridge, MA 02138; Brigham Young University, Provo, UT 84602
89 B9 Spatiotemporal gradients of retinoic acid and identification of target tissues. G. Duester, N. Molotkova, A. Molotkov, O. Sirbu. Burnham Institute, La Jolla, CA
90 B10 Deciphering roles of PDGF targets in vivo. J.P. Schmahl, P. Soriano. Fred Hutchinson Cancer Research Center, Seattle, WA 98109
91 B11 Context-specific requirements for FRS2-mediated signaling downstream of FGFR1 during mouse development. R.V. Hoch, P. Soriano. FHCRC, UW, Seattle, WA 98109
92 B12 IFT proteins regulate both the activator and repressor activities of Gli proteins. A. Liu, B. Wang, L.A. Niswander. University of Colorado Health Sciences Center, Aurora, CO 80045; Cornell University Medical College, New York, NY 10021; HHMI and University of Colorado Health Sciences Center, Aurora, CO 80045
93 B13 A novel secreted regulator of the Hedgehog pathway required for both activation and inhibition. J.F. Reiter. University of California, San Francisco, CA 94143
94 B14 Analysis of the interactions between Wnt-3a and Wnt-5b signaling pathways during chick development. K.J. Sanders, Y. Martinez, M. Meyerzon, L.W. Burrus. SFSU, 1600 Holloway Ave., SF, CA 94132
95 B15 Induction of the opthalmic trigeminal placode is regulated by Wnt/Frizzled signaling. R.T. Lassiter, M.R. Stark. Brigham Young University, UT
96 B16 Wnt-7a is involved in Bmp-2-induced chondro-inhibitory mechanism in chick wing bud mesenchymal cells. E. Jin, S. Lee, Y. Lee, O. Bang, S. Kang. Department of Biological Sciences, Kyungpook National University, Daegu, 701-702, South Korea; Department of Life Sciences, Seoul National University, Seoul 151-742, South Korea 548 Developmental Biology Volume 283, 2005
97 B17 Planar cell polarity patterns the actin-based cell protrusions that form ventral denticles in the Drosophila embryo. J. Walters, S. Dinardo. University of Pennsylvania, Philadelphia, PA
98 B18 TGF-h-induced up-regulation of MMP-2 depends on MAPK signaling in the chondrogenic differentiation of chick leg bud mesenchymal cells. Y. Choi, J. Park, J. Son, E. Jin, S. Kang. Department of Biological Sciences, Kyungpook National University, Daegu, 701-702, South Korea
99 B19 Methyl-Beta-Cyclodextrin inhibits chicken embryo somitic myogenesis possibly through inactivation of lipid raft signaling. W.L. Rosenthal, W.F. Denetclaw. San Francisco State University, San Francisco, CA 94132
100 B20 Dermomyotome filopodia contacts surface ectoderm during early epaxial myotome development in the chicken embryo. G.A. Gomez, E. Okada, W.F. Denetclaw Jr. Department of Biology, San Francisco State University, San Francisco, CA 94132
101 B21 Interaction of Contactin and notochord-derived chondroitin sulfate proteoglycans induces repulsive axon guidance of primary sensory neurons in Xenopus embryo. N. Fujita, S. Nagata. Faculty of Science, Japan Women’s University, Tokyo, Japan
102 B22 Ectodermal GATA-2 functions as part of a transcriptional complex downstream of BMPs during primitive hematopoiesis. G. Dalgin, J.L. Christian. Oregon Health and Science University, Portland, OR 97239
103 B23 The role of fgf receptors in otic placode development. R. Esterberg, A. Fritz. Emory University, Atlanta, GA 30322
104 B24 An early role for Notch signaling during development of the zebrafish inner ear. A. Bermange, N. Daudet, J. Leslie, R. Brooker, J. Lewis. Vertebrate Development Laboratory, Cancer Research UK, 44 Lincoln’s Inn Fields, London WC2A 3PX, UK
105 B25 Fz activity in planar cell polarity signaling is regulated by Wnt(s) gradient. J. Wu, M. Mlodzik. Mt Sinai School of Medicine, NY10029
106 B26 Teaching an old junction new tricks: apical polarization of the septate junction patterns the Drosophila wing independently of Frizzled signaling. D.R. Venema, V.J. Auld. Trinity Western University, Langley BC Canada; University of British Columbia, Vancouver BC, Canada
107 B27 Specificity vs. redundancy: rhomboid1, not roughoid, is required for epidermal growth factor receptor mediated photoreceptor cell patterning. D.A. Birkholz, W. Chou, M. Phistry, S.G. Britt. University Colorado School of Medicine, Aurora, CO 80045; University Texas Health Science Center, San Antonio, TX 78245
108 B28 dLgl functions in differentiating CNS cells. S. Trunova, A. Shearn. Johns Hopkins University, Biology Dep., Baltimore, MD 21218
109 B29 The role of EGF and TGF-h signaling in patterning of follicle cells during Drosophila oogenesis. B.V. Shravage, S. Roth. University of Koeln, Koeln, Germany
110 B30 Action and components of the FAT signaling pathway. E. Cho, K.D. Irvine. HHMI, Waksman Institute, Rutgers University, Piscataway, NJ
111 B31 An investigation of a network of protein interactions downstream of LRP proteins in mouse and Drosophila may also operate in C. elegans. B.J. Avery, S. Vroman, R. Larsen, G.M. Rubin. Dept. of Biology, Westminster College, Salt Lake City, UT 84105, USA; HHMI, Dept. of MCB, U. of CA, Berkeley, CA 94720, USA
112 B32 Two receptor-like kinases mutually required for Arabidopsis embryo development. M. Nodine, K. Davies, R. Yadegari, F. Tax. University of Arizona, Tucson, AZ
Intracellular Signaling Pathways
113 B33 Novel function of POSH, a JNK scaffold, as a specific E3 ubiquitin ligase for the Hrs stability on early endosomes. G. Kim, E. Park, Y. Kong, J. Han. Division of Molecular and Life Sciences, Pohang University of Science and Technology
114 B34 Analysis of the five intracellular PPPSP motifs of LRP6. B.T. MacDonald, K. Tamai, X. Zen, X. He. Division of Neuroscience, Children’s Hospital, Harvard Medical School, Boston, MA 02115
115 B35 Differential control of death and differentiation in myoblasts mediated by Hic-5 isoforms. Z. Gao, L.M. Schwartz. Molecular and Cellular Biology Program, University of Massachusetts, Amherst, MA 01003, USA; Biology Department, University of Massachusetts, Amherst, MA 01003, USA; Pioneer Valley Life Sciences Institute, Springfield, MA 01107, USA
116 B36 Lipid rafts regulate myoblast differentiation and myotube formation in chicken embryo skeletal muscle cultures. A. Odobescu, A. Luna Jr., J. Greenberg, W.F. Denetclaw Jr. Department of Biology, San Francisco State University, San Francisco, CA 94132
117 B37 Interaction between epithelium and myogenic progenitor cells is required for tongue morphogenesis. Y. Taya, T. Fujitake, T. Hirano, D. Torii, Y. Shimazu, K. Sato, Y. Soeno, T. Aoba. Nippon Dental University, Tokyo, Japan SDB Meeting Abstracts 549
118 B38 Hedgehog signaling through the Smoothened complex. J.E. Hooper, C. Bankers, H. Yang. University of Colorado School of Med., Aurora, CO 80045
119 B39 Individual and cooperative activities of the Gli transcription factors in Hedgehog signaling. R. Lipinski, W. Bushman. University of Wisconsin, Madison, WI 53792
120 B40 Sister of open brain encodes an intraflagellar transport protein required to prevent ligand-independent activation of mammalian Hedgehog pathway. J.T. Eggenschwiler, J. Qin. Princeton University, Princeton, NJ 08540
121 B41 Wnt pathways and sea urchin development. J. Croce, D.R. McClay, L. Duloquin, G. Lhomond, C. Gache. Duke University, Durham, NC 27707; UMR7009 CNRS-UPMC, Villefranche-sur-Mer 06230, France
122 B42 SYS-1, a novel h-catenin, is a limiting transcriptional co-activator of POP-1/TCF in C. elegans. A.R. Kidd III, J.A. Miskowski, K.R. Siegfried, J. Kimble. University of Wisconsin-Madison; University of Wisconsin-Madison and HHMI, Madison, WI 53706
123 B43 SUMO conjugase, Lesswright, regulates larval hematopoiesis through Rel-related transcription factors in Drosophila melanogaster. S. Tanda, L. Huang. Ohio University, Athens, OH 45701
124 B44 The adaptor protein X11La/Dmint1 interacts with the PDZ-binding domain of the cell recognition protein Rst in Drosophila. S. Vishnu, A. Hertenstein, G.H. de Couet, K. Fischbach. Institut fu¨r Biologie III, Scha¨nzlestr.1, 79104 Freiburg, Germany; Department of Zoology, University of Hawaii, 2538 McCarthy Mall, Honolulu, HI 96822, USA
125 B45 EphA4 signaling suppresses Cdc42 and RhoA activities by recruiting Pak1 in order to regulate blastomere association in the Xenopus blastula embryo. N. Bisson, L. Poitras, A. Mikryukov, M. Tremblay, T. Moss. Cancer Research Centre, Laval University, Que´bec City, G1R 2J6, Canada
126 B46 EphA9 inhibits precocious EMT during avian gastrulation. K.M. Hardy, R.K. Baker, T.A. Yatskievych, Y.J. Shin, S.L. Greenhut, P.B. Antin. University of Arizona, Tucson, AZ
127 B47 Genetic modifiers of the anti-apoptotic functions of Bcl-2. C.A. Jette, D.M. Langenau, R.A. Stewart, J.P. Kanki, A. Look. Dana-Farber Cancer Institute, Boston, MA 02115
128 B48 Nrarp functions to modulate neural crest cell differentiation by regulating LEF1 protein stability. M. Itoh, T. Ishitani, K. Matsumoto, A.B. Chitnis. University of Nagoya, Aichi 464-8602, Japan; NIH, Bethesda, MD 20892
129 B49 Developmental function of Xp120-catenin in complex with Xenopus Ewing Sarcoma protein. K. Cho, T.G. Vaught, H. Ji, P.D. McCrea. University of Texas M.D. Anderson Cancer Center, GSBS Program in Genes and Development, Houston, TX 77030
130 B50 When pathways collide: inhibitory crosstalk and the spatial regulation of FGF signaling by BMP4. A.K. Sater, H.P. Gohil, C. Liu. University of Houston, Houston, TX 77204
131 B51 Role of Rap2 GTPase in Nodal/Activin signaling pathway. S. Choi, J. Han. Pohang University of Science and Technology, Pohang, 790-784, South Korea
132 B52 LPA signaling in Xenopus laevis requires Xrho and Xrac, but not Xcdc42. R.B. Lloyd, Q. Tao, S.A. Lang, C.C. Wylie. Children’s Hospital Research Foundation, Cincinnati, OH 45229
133 B53 Sprouty1 is a critical regulator of kidney development by antagonizing GDNF/Ret-mediated signals during ureteric bud morphogenesis. M. Basson, S. Akbulut, J. Watson-Johnson, R. Shakya, R. Simon, T.J. Carroll, I. Gross, T. Lufkin. Mount Sinai School of Medicine, New York; Columbia University, New York; Harvard University, Boston; University of California, San Francisco; King’s College London, UK
134 B54 The nuclear envelope protein Man1 is required for angiogenesis in the embryonic yolk sac. T.V. Cohen, C.L. Stewart. National Cancer Institute at Frederick, Frederick, MD 21702
135 B55 Characterization of Chibby knockout mice. V.A. Voronina, K. Takemaru, P.M. Treuting, R.T. Moon. University of Washington, Seattle, WA 98195; HHMI; SUNY at Stony Brook, Stony Brook, NY 11794
Gene Regulation
136 B56 A transgenic approach to understanding imprinting regulation in the PWS locus. C.R. Futtner, K.A. Johnstone, C.I. Brannan, J.L. Resnick. University of Florida, Gainesville, FL
137 B57 The 5V flanking region of Gtl2 controls imprinting and expression of the Gtl2 and Dlk1 genes in the mouse. E. Steshina, M. Carr, J.V. Schmidt. University of Illinois at Chicago, IL 550 Developmental Biology Volume 283, 2005
138 B58 14Gso: A translocation mutant mouse with a disruption of the Beckwith-Wiedemann Syndrome imprinted region. C.M. Elso, A.M. Tarver, X. Lu, L.J. Stubbs. Lawrence Livermore National Laboratory, Livermore, CA 94550
139 B59 Implantation Serine Protease 1 and 2. L. Tang, S. Liu, C. O’Sullivan, D. Rancourt. University of Calgary, Calgary, AB T2N4N1 Canada
140 B60 A Prep-1 null mutation is embryonic lethal at E7.5. L.C. Fernandez-Diaz, M.T. Fiorenza, N.A. Jenkins, N.G. Copeland, F. Blasi. Universita` Vita Salute San Raffaele, Milan, Italy; IFOM (FIRC Institute of Molecular Oncology), Milan, Italy; National Cancer Institute, Frederick, MD, USA
141 B61 Regulatory mechanisms of H19 DMR at the heterologous Afp locus. Q. Rong, K. Pfeifer. Section on Genomic Imprinting, Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892
142 B62 The homeodomain protein Hhex is a direct repressor of Endothelial Cell-Specific Molecule 1 (ESM-1). R. Cong, X. Jiang, C.M. Wilson, C.W. Bogue. Yale University School of Medicine, New Haven, Connecticut, USA
143 B63 CREB activation in synergy with BMP2 signaling directly regulates Phox2a gene transcription. C. Benjanirut, M. Paris, R. Hullinger, O. Andrisani. Purdue University, West Lafayette, IN 47907
144 B64 Phenotypic and epigenetic analysis of SUZ12 deficient mice. S.J. Chamberlain, T. Magnuson. University of North Carolina-Chapel Hill, Chapel Hill, NC 27599
145 B65 Chromatin remodeling on promoters of late muscle marker genes during myogenesis. Y. Ohkawa, C.G. Marfella, A.N. Imbalzano. University of Massachusetts Med School, MA 01655
146 B66 Identifying gene targets of myogenin in embryonic and post-natal skeletal muscle. E. Meadows, J. Davie, J.R. Knapp, W.H. Klein.UTMD Anderson Cancer Center
147 B67 Long-range regulation of Hoxa13 in limb development. J.A. Lehoczky, M.E. Williams, J.W. Innis. University of Michigan, Ann Arbor, MI 48109
148 B68 Pulmonary expression and transcriptional regulation of a7 Nicotinic Acetylcholine receptors by TTF-1 and Egr-1 during development. P.R. Reynolds, J.R. Hoidal. University of Utah, SLC, UT 84132
149 B69 Characterization of OTK18 regulation on neuronal survival. S.L. Bogers, K.A. Carlson. University of Nebraska at Kearney, Kearney, NE 68849-1140
150 B70 Functions of Brn3b transcription factor in mouse retinal ganglion cell development. L. Pan, Z. Yang, L. Gan. Center for Aging and Developmental Biology, University of Rochester, Rochester, NY 14642
151 B71 TGFß and Wnt regulation of cell proliferation and extracellular matrix synthesis in murine embryonic maxillary mesenchymal cells. D.R. Warner, H.S. Smith. Birth Defects Center, Department of Molecular, Cellular, and Craniofacial Biology, University of Louisville; Department of Pediatrics, University of Louisville, Louisville, KY 40292
152 B72 Pax6 DRR is required to coordinate the interaction of widely spaced regulatory elements. J. Kim, J.D. Lauderdale. University of Georgia, Athens, GA 30602
153 B73 Analysis of Pax6 regulation using a comparative approach. A. Majumder, J.D. Lauderdale. University of Georgia, Athens, GA 30605
154 B74 T-box transcription factor specificity in the zebrafish mesoderm. A.T. Garnett, T.M. Han, M.B. Eisen, S.L. Amacher. University of California, Berkeley
155 B75 DNA methylation reprogramming during embryonic development in zebrafish. A.B. MacKay, A.A. Mhanni, P.H. Krone. University of Saskatchewan, Saskatoon, SK, Canada; University of Manitoba, Winnipeg, MB, Canada
156 B76 Regulating GAP-43 expression in the developing and regenerating nervous system: a comparative genomics approach. A.J. Udvadia. University Wisconsin-Milwaukee
157 B77 Genomic organization and functional analysis of the zebrafish methyl-cytosine binding protein 2 (MeCP2) gene. L.E. Coverdale, C. Martyniuk, V. Trudeau, C.C. Martin. Department of Biology, Center for Advanced Research In Environmental Genomics, University of Ottawa, Ottawa, Ontario, Canada
158 B78 Role of histone deacetylase 3 in zebrafish heart development and growth. S. Zchut, R. Creton, L. Kochilas. Rhode Island Hospital, Providence, RI 02905; Brown University, Providence, RI 02912
159 B79 Kaiso/ p120-Catenin and TCF/ beta-catenin complexes coordinately regulate canonical Wnt gene targets. J. Park, J.P. Lyons, H. Ji, T. Nguyen, M.C. Barton, T. Deroo, K. Vleminckx, P.D. McCrea. University of Texas M. D. Anderson Cancer Center, GSBS, Houston, TX 77030; Ghent University, B-9052 Ghent, Belgium SDB Meeting Abstracts 551
160 B80 Early Wnt signaling and later BMP signaling regulate geminin expression in the presumptive neurectoderm. J.J. Taylor, K.L. Kroll. Washington University St. Louis, MO 63110
161 B81 Regulation of SoxB1 genes in neural induction in Xenopus laevis. C. Rogers, E. Casey. Georgetown University, Washington, DC 20057
162 B82 Regulation of Ath5 gene expression in the vertebrate neural retina. M.I. Hanson, D.A. Hutcheson, M.L. Vetter. University of Utah
163 B83 Identification of functionally conserved regulatory regions upstream of vertebrate Optx2 genes. M. Zuber, M. Theisen, A. Viczian. Departments of Ophthalmology and Biochemistry and Molecular Biology, SUNY Upstate Medical University; Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY
164 B84 Characterization of alpha globin genes in Xenopus tropicalis. D.D. Roche, R.M. Harland. University of California, Berkeley, CA 94720
165 B85 Identification of chicken odd-skipped related 1 (osr1) regulatory elements. A.V. Petrova, T.M. Schultheiss. Harvard Medical School and Beth Israel Deaconess Medical Center, Boston, MA 02215
166 B86 Genomic analysis of placode formation. K.L. McCabe, A. Manzo, L.S. Gammill, M. Bronner-Fraser. California Institute of Technology, Pasadena, CA 91125
167 B87 Transcription regulation of Drosophlia neuroblast temporal determinants. T. Brody, A. Kuzin, W. Odenwald. National Institutes of Health, Bethesda, MD 20892
168 B88 Analysis of Nerfin-1 transcriptional and post-transcriptional regulation. A. Kuzin, T. Brody, W. Odenwald. Neural Cell-Fate Determinants Section, NINDS, NIH, Bethesda, MD 20892-3706
169 B89 Dpp expression and function in the posterior spiracle: direct activation by combinatorial activity of the Dpp and Wg pathways and independence from Dpp function in dorsal-ventral patterning. S.J. Newfeld, N.T. Takaesu. School of Life Sciences, Arizona State University, Tempe, AZ 85287-4501
170 B90 Regulation of Snail transcription: a chromatin structure approach. M.B. Palmer, J.M. Boss, P.A. Wade. Emory University, Atlanta, GA 30322; NIEHS, Research Triangle Park, NC 27709
171 B91 Morpholino oligonucleotide knock-down of Slug reveals a number of potential downstream target genes. L.A. Taneyhill, E.G. Coles, M. Bronner-Fraser. California Institute of Technology, Division of Biology, Pasadena, CA 91125, USA
172 B92 The mechanism of maternal mRNA localization in the early Drosophila embryo. J.L. Semotok, H.D. Lipshitz. Dept. of Mol. and Med. Genetics, University of Toronto, Prog. in Dev. Biol., HSC, Toronto, Canada
173 B93 Characterization of the C. elegans hypoxia-inducible factor and its regulators. J. Powell-Coffman, C. Shen, S. McMaken, K. Gillette. Iowa State University, Ames, IA
Functional Genomics
174 B94 The Prep-1 interactome and a novel inhibitor of Prep-PBX activity, P160 Myb-Binding-Protein. V.M.Diaz Cortes, A. Bachi, F. Blasi. University Vita Salute San Raffaele, DIBIT, Milan, Italy; University Vita Salute San Raffale and IFOM (FIRC Institute of Molecular Oncology), Milan, Italy
175 B95 Functionality of JcDNV-derived somatic transformation vectors in insects and the role of viral enhancer sequences. P.D. Shirk, R.B. Furlong, J. Gillett, H. Bossin. USDA ARS CMAVE, Gainesville, Florida; University of Florida, Gainesville, FL; FAO/IAEA Agriculture and Biotechnology Laboratory, Seibersdorf, Austria
176 B96 The zebrafish kohtalo/trap230 gene is required for the development of the brain, neural crest and pronephric kidney. S. Hong, C.E. Haldin, P.E. Ulanch, N.D. Lawson, B.M. Weinstein, I.B. Dawid, N.A. Hukriede. NIH, Bethesda, MD20892; University of Pittsburgh School of Medicine, Pittsburgh, PA 15261
177 B97 GEISHA is the Gallus Expressed Sequence Tag In Situ Hybridization Analysis. D. Darnell, G. Bell, S. Kuar, P. Antin. University of Arizona Health Sciences Center, Department of Cell Biology and Anatomy, Tucson, AZ
178 B98 Genome-wide expression profiling of Xenopus laevis metamorphosis programs. B. Das, L. Cai, M.G. Carter, A.A. Sharov, Y. Piao, M.S. Ko, D.D. Brown. Department of Embryology, Carnegie Institution of Washington, 115 W University Pkwy., Baltimore, MD 21210; Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, Sui
179 B99 A genetic map for Xenopus tropicalis. D. Wells, L. Gutierrez, Z. Xu, L. Bellot, Y. Yuan, M. Hitchens, A.K. Sater, S.E. Scherer. Dept. of Biology and Biochemistry, University of Houston, Houston, TX; Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX 552 Developmental Biology Volume 283, 2005
180 B100 The Mouse Atlas of Gene Expression: SAGE analysis of preimplantation stages. M.K. Wu, R. Cullum, J.L. Rupert, A. Siddiqui, A. Delaney, A. Charters, J. Khattra, S. Jones. Terry Fox Laboratory, B.C. Cancer Agency, Vancouver, Canada; Genome Sciences Centre, B.C. Cancer Agency, Vancouver, Canada
181 B101 Sage analysis of early mouse endoderm. J. Hou, M.K. Wu, A. Charters, P.A. Hoodless. Terry Fox laboratory, B.C. Cancer Agency, Vancouver, B.C., Canada; Genome Science Center, B.C. Cancer Agency, Vancouver, B.C., Canada
182 B102 Microarray analysis of transcriptomes in normal developing mouse autopods and Hoxa13 knockout mouse model of Hand–Foot–Genital Syndrome. S. Shou, S.H. Stadler. Shriners Hospital for Children, Research Division, 3101 SW Sam Jackson Park Road, Portland, OR 97239; Oregon Health and Science University, Department of Molecular and Medical Genetics, 3181 SW Sam Jackson Park Road, Portland, OR 97239
183 B103 Expression of Wnt5a gene in mouse embryonic orofacial development. J. Xiao, E. Zhu, N. Nagai, H. Nagatsuka, C. Li. Dalian Medical University, Dalian 116027, China; Okayama University, Okayama City 700-8525, Japan; University of Southern California, CA 90033
184 B104 The mouse hair growth cycle as a model for studying cyclic biological processes. K.K. Lin, D. Chudova, P. Smyth, B. Andersen. Departments of Biological Chemistry, Medicine and Computer Sciences, University of California, Irvine, CA 92697
185 B105 Withdrawn
186 B106 Genetic pathways that regulate early development of the mouse embryo and neural tube closure. I.E. Zohn, A. Pollock, K.V. Anderson, L. Niswander. Developmental Biology Program, Sloan-Kettering Institute, New York, NY 10021; Howard Hughes Medical Institute, Department of Pediatrics, Section of Developmental Biology, University of Colorado Health Sciences Center, Aurora, CO 80045
187 B107 Overexpression of human Sclerostin (Sost) in mice impairs limb development. N.M. Collette, J. Chang, R.M. Harland, G.G. Loots. Genome Biology Division, Lawrence Livermore National Laboratory, Livermore, CA, USA; Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
188 B108 Identification of genes with oscillatory expression in mouse somitogenesis using a synchronized human stem cell model. D.A. William, B. Saitta, V. Markov, D.A. Hall, J.D. Gibson, M.S. Mimoto, E.F. Rappaport, K. Kusumi. The Children’s Hospital of Philadelphia and University of Pennsylvania School of Medicine, Philadelphia, PA 19104; The Coriell Institute for Medical Research, Camden, NJ 08103
Cell Proliferation
189 B109 Molecular dissection of the C. elegans DTC leader and niche cell functions. D. Byrd, D. Hesselson, J. Kimble. HHMI and Department of Biochemistry, University of Wisconsin-Madison; Dept. of Genetics, University of Wisconsin-Madison, Madison, WI 53706
190 B110 Identification of factors mediating cell competition in Drosophila melanogaster. C. de la Cova, M. Tu, L.A. Johnston. Genetics and Development, Columbia University, New York, NY 10032
191 B111 Control of growth in Drosophila head by elbow and no ocelli. C.M. Luque, M. Mila´n. Institucio´ Catalana de Recerca i Estudis Avanc¸ats (ICREA); Institut de Recerca Biome`dica, Parc Cientı´fic de Barcelona, Spain, EU
192 B112 Frizzled-10 is required for the expansion of the dorsal neural tube during chick development. A.N. Easton, R.N. Munji, L.M. Galli, R.O. Suriben, M.R. Stark, L.W. Burrus. San Francisco State University, San Francisco, CA 94132; Brigham Young University, Provo, UT 84602
193 B113 lbx1 promotes myoblast proliferation through down-regulation of myoD. B.L. Martin, R.M. Harland. University of California, Berkeley
Early Embryo Patterning
194 B114 Efflux transporters at the crossroads of development and the environment. A. Hamdoun, D. Epel. Hopkins Marine Station, Stanford University, Oceanview Blvd. Pacific Grove CA 93950, USA
195 B115 Multiple roles for snail in mesoderm development of the sea urchin embryo. S. Wu, D.R. McClay. Department of Biology, Duke University, Durham, NC 27708, USA
196 B116 Differential protein turnover, negative autoregulation, and transcriptional repression precisely regulate SoxB1 levels in the early sea urchin embryo. L.M. Angerer, L.A. Newman, R.C. Angerer. NIDCR/NIH, Bethesda, MD 20892; University Rochester, Rochester, NY 14634
197 B117 Dishevelled regulates nuclear ß-catenin during germ layer segregation in the cnidarian, Nematostella vectensis. S. Kumburegama, A. Wikramanayake. Department of Zoology, University of Hawaii at Manoa, Honolulu, HI 96822 SDB Meeting Abstracts 553
198 B118 C. elegans non-muscle myosin regulates apicobasal par-3 distribution and blastocoel size. J. Nance, K. Good, R. Cinalli, K. Wachter, J.R. Priess. Skirball Institute, NYU School of Medicine, New York, NY 10016; Fred Hutchinson Cancer Research Center and HHMI, Seattle, WA 98109
199 B119 Secondary axis formation by transplantation of D-quadrant micromeres in the Tubifex embryo. A. Nakamoto, T. Shimizu. Dept. of MCB, University of Arizona; Division of Biological Sciences, Graduate School of Science, Hokkaido University
200 B120 Regulating mRNA distribution in the early Ilyanassa embryo. J. Wandelt, J. Cooley, M. LaGioia, T. Oberg, L.M. Nagy. University of Arizona, Tucson, AZ 85721
201 B121 Early embryonic patterning in the amphipod crustacean, Parhyale hawaiensis. M.S. Modrell, A.L. Price, R. Hannibal, N.H. Patel. University of California-Berkeley; University of Chicago; HHMI
202 B122 Analysis of hedgehog signaling during segmentation in Tribolium casteneum. L. Farzana, S.J. Brown. Kansas State University, Manhattan, KS 66506
203 B123 Identification of a new variant (TH-B) of tumorhead, a cell proliferation activator from Xenopus. E.E. Traverso, M. Cho, C. Wu, L.D. Etkin. University of Texas M.D. Anderson Cancer Center, Houston, TX 77030
204 B124 Analysis of Sox3 function in early neurogenesis of Xenopus laevis. N. Harafuji, E.M. Casey. Georgetown University, Washington, DC 20057
205 B125 Fgf8 spliceforms differ in activity and are involved in both mesoderm and posterior neural formation in Xenopus laevis. R.B. Fletcher, R.M. Harland. University of California-Berkeley
206 B126 Xenopus Gut Macroglobulin-1, a novel alpha-2 Macroglobulin, is expressed in a restricted dorsal domain of the developing primitive gut tube. L.L. Pineda Salgado, E. Craig, R. Blank, D.S. Kessler. University of Pennsylvania, PA 19104
207 B127 Notch and Shh signaling in dorsal midline patterning in Xenopus laevis. S.M. Peyrot, Y. Hafezi, J.B. Wallingford, R.M. Harland. University of California, Berkeley, CA 94720; University of Texas, Austin, TX 78712
208 B128 BMP antagonists are required in Spemann’s Organizer for dorsal patterning. M.K. Khokha, J. Yeh, T.C. Grammer, R.M. Harland. UC- Berkeley, Berkeley, CA 94720
209 B129 Xenopus protein-O-fucosyltransferase 1 is important for posterior patterning. C. Yeo, H. Lee, Y. Kim. Dept. Life Sciences and Center for Cell Signaling Research, Ewha Womans University, Seoul 120-750, South Korea
210 B130 Maternal XTcf1 and XTcf4 have distinct functions and are required for axial development of the Xenopus embryo. H.J. Standley, J. Heasman. Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, UK; Cincinnati Children’s Hospital Research Foundation, Cincinnati, OH 45229, USA
211 B131 Groucho regulation of Nodal signaling in the Xenopus embryo. A.B. Steiner, S. Yaklichkin, D.S. Kessler. University of Pennsylvania School of Medicine, Philadelphia, PA 19104
212 B132 Temporal analysis of the early BMP functions: distinct anti-organizer and mesoderm patterning phases. A. Fainsod, K. Marom, V. Levy, G. Pillemer. Hebrew University, Jerusalem 91120, Israel
213 B133 Microarray dissection of Spemann-Mangold organizer function. A.L. Hufton, J.C. Baker. Stanford University, Stanford, CA 94305, USA
214 B134 Establishing frog genetics and the identification of mutants in Xenopus tropicalis. T.C. Grammer, M.K. Khokha, M.A. Lane, K. Lam, R.M. Harland. Molecular and Cell Biology, UC Berkeley; Center for Integrated Genomics, UC Berkeley; Department of Pediatrics, UCSF School of Medicine
215 B135 Ecsox17 expression in the early embryo of a direct developing frog. S. Singamsetty, S.R. Williamson, R.P. Elinson. Duquesne university, Pittsburgh, PA 15282
216 B136 Zebrafish KLF4 is essential for hatching and haematopoiesis. M.R. Gardiner, D.F. Daggett, S.L. Amacher, P.D. Currie, L.I. Zon, A.C. Perkins. Institute for Molecular Biosciences, Queensland University, Australia; Department of Molecular and Cellular Biology, University of California, Berkeley, California; Victor Chang Cardiac Research Institute, Sydney, Australia; Children’s Hospital, Harvard, MA
217 B137 Novel Dvl2 interacting protein Crip2 regulates notochord cell intercalation of zebrafish embryo. Y. Kida, T. Sato, T. Ogura. IDAC, University of Tohoku, Japan
218 B138 Morpholino Knockdown uncovers a requirement for FoxH1/Schmalspur for normal morphogenesis and survival during gastrulation. W. Pei, B. Feldman. Medical Genetics Branch, National Human Genome Research Institute, Bethesda, MD 20892 554 Developmental Biology Volume 283, 2005
219 B139 FoxD3 regulation of mesoderm induction in the zebrafish embryo. L.L. Chang, D.S. Kessler. University of Pennsylvania School of Medicine, Philadelphia, PA 19104
220 B140 Twisted gastrulation promotes BMP signaling in zebrafish DV axial patterning. S.C. Little, M.C. Mullins. University of Pennsylvania, Philadelphia, PA 19104
221 B141 Temporal progression of BMP signaling in dorsoventral patterning of the early zebrafish embryo. J.A. Tucker, M.C. Mullins. Dept. of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA
222 B142 BMP patterning activities can be defined in zebrafish according to their dependence on Wnt8. A.C. Lekven, M. Ramel, G.R. Buckles, K.D. Baker. Texas A & M University, College Station, TX 77843
223 B143 Dlx3b/4b act to prolong otic competence by restricting BMP signals from the neural plate. R. Esterberg, A. Fritz. Emory University, Atlanta, GA 30322
224 B144 The role of insulin-like growth factor-2 (IGF-2) in zebrafish embryonic development. K. McCarthy, C. Glynn, L. Hartnett, C. Nolan, L. Byrnes. National University of Ireland, Galway, Ireland.; University College Dublin, Ireland
225 B145 Zic2 function in the embryonic zebrafish forebrain. N.A. Sanek, M.K. Nyholm, Y. Grinblat. Genetics Training Program; Depts. of Anatomy and Zoology; University of Wisconsin, Madison
226 B146 Characterization of zic1 expression in early chick development. L. Sun Rhodes, R. VanTreese, C. Merzdorf. Montana State University, Bozeman
227 B147 Defining organ-position along the main axis of the gut. E. Bayha, A. Grapin-Botton. ISREC, Chemin des Boveresses 155, CH-1066 Epalinges s/Lausanne, Switzerland
228 B148 FGF-signaling is necessary for establishing gut tube domains along the anterior-posterior axis in vivo. J.M. Wells, J. Dessimoz, R. Opoka, J. Kordich, A.M. Zorn, A. Grapin-Botton. Cincinnati Children’s Hospital Research Foundation, OH, USA; ISREC, Lausanne, Switzerland
229 B149 Cloning and characterization of a new chick Hairy/Enhancer of Split homologue. R.P. Andrade, S. Santos, I. Palmeirim. Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal
230 B150 Temporal control of limb bud development: evidence of a molecular clock. S. Pascoal, C. Carvalho, I. Palmeirim. Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal; Centro de Biologia do Desenvolvimento, Instituto Gulbenkian de Cieˆncia, Oeiras, Portugal
231 B151 Terra is a left-right asymmetry gene crucial to maintaining bilateral symmetry of the segmentation clock. S. Leonor, R. Lourenc¸o, A. Gonc¸alves, I. Palmeirim. Centro de Biologia do Desenvolvimento, Instituto Gulbenkian de Cieˆncia, Oeiras, Portugal; Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal
232 B152 A microarray-based approach of somitogenesis. M. Dequeant, E. Glynn, M. Wahl, J. Chen, G. Glazko, A. Mushegian, O. Pourquie. Stowers Institute For Medical Research
233 B153 Ichabod is required for forebrain formation in the mouse embryo. X. Zhou, K. Anderson. Weill Graduate School of Medical Sciences, Cornell University, 445 E69th St. New York, NY 10021; Memorial Sloan Kettering Cancer Institute, 1275 York Ave. New York, NY 10021
234 B154 Genetic dissection of midbrain and anterior hindbrain development. M. Zervas, A. Joyner. Developmental Genetics Program, Skirball Institute, NYU School of Medicine, New York, NY 10016
235 B155 Shifting boundaries of retinoic acid activity control hindbrain segmental gene expression. I. Sirbu, L. Gresh, J. Barra, G. Duester. The Burnham Institute, La Jolla CA 92037, USA; Institute Pasteur, Paris, France
236 B156 The role of BMP antagonism by the Organizer in mammalian neural induction. Y. Yang, J. Klingensmith. Department of Cell Biology, Duke University Medical Center, Durham, NC 27710
237 B157 Neural tube patterning and the role of BMP2. T. Castranio, Y. Mishina. Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709
238 B158 A balance of FGF, BMP and Wnt signalling positions the sensory placode territory in the head. A. Streit, A. Litsiou, H. Sven. Dept. Craniofacial Development, King’s College London, London, UK
239 B159 Hydrodynamic basis of nodal flow: de novo formation of left-right asymmetry in mouse development. S. Nonaka, S. Yoshiba, D. Watanabe, S. Ikeuchi, T. Goto, W.F. Marshall, H. Hamada. UCSF, San Francisco, CA 94143-2200; Osaka University, Osaka 565-0871, Japan; Kitasato University, Kanagawa 228-8555, Japan; Tottori University, Tottori 680-8552, Japan SDB Meeting Abstracts 555
240 B160 Requirement of the anterior visceral endoderm for the establishment of anterior-posterior axis and mesoderm patterning. S. Miura, Y. Mishina. Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
241 B161 Multiple roles for BMP antagonism in left-right asymmetry of mouse embryos. T.J. Sitzman, R.M. Anderson, J. Klingensmith. Duke University Medical Center, Durham, NC 27710
242 B162 BMP signaling is required in the LPM to block Nodal expression on the right side of the mouse embryo. M.J. Solloway, M.B. Furtado, E.J. Robertson, R.P. Harvey. Victor Chang Cardiac Res. Institute, Darlinghurst, NSW Australia 2010; Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX2 7BN, UK
243 B163 Down-regulation of Geminin by RNAi in the early post-implantation mouse embryo. L.S. De Boer, T.E. Gratsch, S. O’Shea. Department of Cell and Developmental Biology, University of Michigan
244 B164 SPC-independent Nodal signaling during early mouse embryogenesis. N. Ben-Haim, M. Guzman-Ayala, D.B. Constam. Developmental Biology Group, Swiss Institute for Experimental Cancer research (ISREC), Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland
245 B165 Cdx2 is required for maintenance of the trophectoderm in the mouse blastocyst. A. Ralston, D. Strumpf, Y. Yamanaka, K. Chawengsaksophak, J. Rossant. Samuel Lunenfeld Research Institute, Toronto, ON M5G 1X5, Canada
246 B166 Evidence for positive and negative regulation of the mouse Cdx2 gene. C.S. Shashikant, W.C. Wang. The Pennsylvania State University, University Park, PA 16802
247 B167 Characterization of cell potential in the early mouse embryo. R.O. Stephenson, F.H. Chen, Y. Yamanaka, J. Rossant. University of Toronto and Samuel Lunenfeld Research Institute, Toronto, ON, Canada; Samuel Lunenfeld Research Institute, Toronto, ON, Canada
248 B168 Role of Daam genes in the regulation of cell polarity during mammalian embryogenesis. M. Nakaya, L. Tessarollo, R. Habas, X. He, T.P. Yamaguchi. NCI-Frederick/ NIH, Frederick, Maryland 21702, USA; University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA; Children’s Hospital, Harvard Medical School, Boston, MA 02115, USA
249 B169 The functional role of Amnionless in middle primitive streak assembly in mammalian gastrulation. C. Munoz, S. Strope, R. Rivi, T. Metzger, E. Lacy. Developmental Biology, Sloan-Kettering Institute, New York, NY 10021; Molecular Biology, Weill Graduate School/Cornell University, New York, NY 10021
250 B170 Disruption of the mouse La gene leads to failure to develop beyond blastocyst and prohibits embryonic stem cell development. J. Park, M.J. Kohn, M. Bruinsma, C. Vech, I. Mukherjee, S. Fuhrmann, A. Grinberg, P.E. Love. NICHD, NIH, Bethesda, MD 20852; Temple University School of Medicine, Philadelphia, PA 19140
251 B171 Toll-Like Receptor and Relish expressions in human embryonic tissue are age-dependent. H. Hao, P.H. Wooley. Wayne State University
252 B172 Implantation of eight-cell embryos in transgenic mouse production. F. Pan, J. Wang. LSU Health Sciences Center, New Orleans, LA 70112
253 B173 EMAGE, Edinburgh mouse atlas of gene expression. J. Christiansen, L. Richardson, S. Venkataraman, P. Stevenson, N. Burton, Y. Yang, R. Baldock, D. Davidson. MRC Human Genetics Unit, Edinburgh EH4 2XU, UK
254 B174 The mouse gene expression database (GXD): Integrated access to expression information for the laboratory mouse. D.A. Begley, J.H. Finger, T.F. Hayamizu, D.P. Hill, I.J. McCright, C.M. Smith, M. Ringwald, T. Group. The Jackson Laboratory, Bar Harbor, ME 04609
255 B175 Interactions between the cell cycle and embryonic patterning in Arabidopsis uncovered by a mutation in DNA polymerase epsilon. P.D. Jenik, R.J. Jurkuta, K. Barton. Carnegie Institution of Washington, Stanford, CA 94305; University of Wisconsin-Madison, Madison, WI 53706
Cell Fate Specification
256 B176 Regulation of dictyostelium development by presenilin and GSK3 signaling. V.C. McMains, L. Kreppel, A.R. Kimmel. NIDDK/NIH Bethesda, MD 20892; Johns Hopkins University, Baltimore, MD 21218
257 B177 Hedgehog maintains groove cell fate during Drosophila embryogenesis. S. Vincent, J. Axelrod. Stanford University
258 B178 Evidence for maternal contribution of Med and End function to their zygotic roles in endoderm specification in C. elegans. M.F. Maduro, G. Broitman-Maduro, J. Zhu, J. Sumerel, J.H. Rothman. Biology Department, UC Riverside, CA 92508; Department of MCDB, UC Santa Barbara, CA 93106 556 Developmental Biology Volume 283, 2005
259 B179 Elucidating the function and expression of target genes of the C. elegans mesendoderm-specifying genes med-1 and med-2. G. Broitman- Maduro, W.W. Hung, T. Lin, M.F. Maduro. University of California, Riverside, CA 92521
260 B180 RhoA triggers endoderm invagination during sea urchin gastrulation. W.S. Beane, J.M. Gross, D.R. McClay. Duke University, Durham, NC; Harvard University, Cambridge, MA
261 B181 New components of endoderm specification including Bmp4 and SnoN. J. Baker, E. Chiao, J. Leonard, K. Dickinson. Department of Genetics, Stanford University, Stanford, CA 94062
262 B182 The role of Xenopus Tbx2, T-box transcription factor, for lateral plate mesoderm formation. G. Cho, J. Han. Department of Life sciences, Pohang University of Science and Technology, San 31, Hyoja-Dong, Nam-Gu, Pohang 790-784, Republic of Korea (south)
263 B183 An analysis of pluripotency of the dorsal ectoderm and mesoderm in Xenopus. T.A. Sylvester, L.A. Dali, C.R. Domingo. San Francisco State University, San Francisco, CA; Nasa Ames Research Center, Moffatt Field, CA
264 B184 Hairy-2a promotes floor plate fates at the expense of the notochord, blocking involution in the Xenopus organiser. S.L. Lo´pez, M.V. Rosato-Siri, P.G. Franco, A.R. Paganelli, P. Raimondi, A.E. Carrasco. LEM-IBC. School of Medicine, UBA-CONICET, Buenos Aires, Argentina
265 B185 The function of growth/differentiation factor 11 (Gdf11) in rostrocaudal patterning of the developing spinal cord. J. Liu. University of Virginia, Charlottesville, VA 22908
266 B186 Pax6 controls cell fates in the developing spinal cord by regulating Wnt signaling. K. Misra, E. Kuang, S. Li, M.P. Matise. UMDNJ, Piscataway, NJ 08854
267 B187 Analysis of the role of Pax3/7 in mediating Wnt signaling in the chick dorsal neural tube. T.L. Barnes, L.M. Galli, S.R. Knight, A. Kawakami, L.W. Burrus. Department of Biology, SFSU, SF, CA; University of Tokyo, Japan
268 B188 Analysis of the role of porcupine in the formation of the Wnt gradient in the chick neural tube. S.S. Secrest, M.J. Skalak, L.M. Galli, W.T. O’Connor, L.W. Burrus. Dept. of Biology, San Francisco State University, 1600 Holloway Avenue, SF, CA 94132
269 B189 Directly visualizing Shh in the developing CNS of the mouse. C.E. Chamberlain, J. Jeong, B.L. Allen, C. Guo. Harvard University; University of California-San Francisco
270 B190 PTEN and AKT are critical regulators of cell fate and position in the developing murine brain. A.D. Hill, C.A. Walsh. Beth Israel Deaconess Medical Center; Beth Israel Deaconess Medical Center/HMS/HHMI
271 B191 The Rx gene and pituitary gland development. E.A. Kozhemyakina, B. Harfe, P. Mathers. Sensory Neuroscience Research Center, West Virginia University, Morgantown, WV; Dept. of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL
272 B192 Studies on the mechanism of neuronal differentiation by the homeodomain transcription factor Phox2a. M. Paris, O. Andrisani. Purdue University, West Lafayette, IN 47907
273 B193 Regulation of neuronal differentiation by a novel neuron-specific EGF-like repeat domain-containing protein, NELL2. N. Choi, J. Choi, B. Lee. Dept. of Biological Sciences, University of Ulsan, Ulsan 680-749, South Korea
274 B194 Intersectional fate mapping reveals embryonic origins of hindbrain cochlear neurons. A.F. Farago, R.B. Awatramani, S.M. Dymecki. Harvard Medical School, Boston, MA 02115
275 B195 The FGF8 signaling pathway regulates differentiation during the development of the cochlear sensory epithelium. B.E. Jacques, E.M. Layman, M. Lewandoski, M.W. Kelley. Developmental Neuroscience NIDCD-NIH, Bethesda, MD 20892; Cancer and Developmental Biology NCI-NIH, Frederick, MD 21702
276 B196 A role for hedgehog signaling in development of the mammalian cochlear sensory epithelium. E.C. Driver, S.P. Pryor, P. Hill, J. Turner, U. Ru¨ther, L.G. Biesecker, A.J. Griffith, M.W. Kelley. NIDCD, NIH, Bethesda, MD; NIDCD, NIH, Rockville, MD; Heinrich-Heine University, Du¨sseldorf, Germany; NHGRI, NIH, Bethesda, MD
277 B197 Hh signaling regulates formation of Purkinje neurons in the zebrafish cerebellum. K. McFarland, J. Topczewska, B. Appel. Department of Biological Sciences, Vanderbilt University, Nashville, TN; Children’s Memorial Institute for Education and Research, Northwestern University Feinberg School of Medicine, Chicago, IL
278 B198 Zebrafish isl1 promotes motoneuron formation and inhibits interneuron formation. S.A. Hutchinson, J.S. Eisen. University of Oregon, Eugene, OR 97403
279 B199 Identification of domains in Xath5 required for inhibition by Notch signaling. C.M. Dooley, K.B. Moore, M.L. Vetter. University of Utah, Salt Lake City, UT 84132 SDB Meeting Abstracts 557
280 B200 The roles of Xebf3 during Xenopus neurogenesis. Y.S. Green, M.L. Vetter. University of Utah, Salt Lake City, Utah 84132
281 B201 Role of N-Cadherin-mediated cell adhesion in regulating neurogenesis. K. Chalasani, K. John, S. Yeo, A. Chitnis, R. Brewster. UMBC, Baltimore, MD; Watson School of Biological Sciences, CSHL, NY; NIH/NICHD, Bethesda, MD
282 B202 Asymmetry of a cholinergic system in the zebrafish brain. K. Santhakumar, C. Thisse, B. Thisse, M.E. Halpern. Carnegie Institution of Washington, Baltimore, MD 21210, USA; IGBMC, CU de Strasbourg, 67404 Illkirch cedex, France
283 B203 Corneal stromal cells can de-differentiate and contribute to multiple neural crest derivatives. P.Y. Lwigale, P.A. Cressy, M. Bronner-Fraser. California Institute of Technology
284 B204 Cellular and genetic characterization of the zebrafish ENS mutant. M. Guyote, J. Delalande, W.S. Talbot, D.W. Raible. Dept. of Biology, Emory University, RRC 1510 Clifton Rd, Atlanta, GA 30322; Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305; Department of Biological Structure, University of Washington, Box 357420, Seattle WA
285 B205 Zebrafish prdm1 is required for neural crest and sensory neuron specification. K.B. Artinger, L. Hernandez-Lagunas, I.F. Choi, C. Cortez, T. Kaji, P. Simpson. University of Colorado Health Sciences Center, Aurora, CO
286 B206 Role of Egfr/Ras pathway in the specification of sensory organs of the wing margin in Drosophila melanogaster. P. Kshetrapal, S. L.S. Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
287 B207 Late specification of the larval peripheral nervous system in the ascidian Ciona intestinalis. R.W. Zeller, M.A. Pellatiro, A. Okamura, K. LaFontaine. San Diego State University
288 B208 The role of nutrition in creation of the eye imaginal disc and initiation of metamorphosis in Manduca sexta. D.T. Champlin, J. Allee, S.G. MacWhinnie, C.A. Nelson, L.M. Riddiford, J.W. Truman. University of Southern Maine, Portland, ME 04103; University of Washington, Seattle, WA 98195
289 B209 The functional role of Pax-3 in ophthalmic trigeminal placode development. J.R. Bradshaw, M.R. Stark. Brigham Young University, Provo, UT
290 B210 Olfactory and lens placode precursors are independently specified despite a shared spatial origin. S. Bhattacharyya, A. Bailey, A. Streit, M. Bronner-Fraser. California Institute of Technology, Pasadena, CA, USA; King’s College, London, UK
291 B211 Induction and cell fate specification in early eye development. P. Mathers, V. Voronina, E. Kozhemyakina, V. Bennett, B. Zamora, M. Lewandoski. Sensory Neuroscience Research Center, West Virginia U., Morgantown, WV; Genetics of Vertebrate Development Section, NCI, Frederick, MD
292 B212 Role of Dkk2 in the maintenance of ocular surface morphology. M. Mukhopadhyay, M. Morasso, C. Ma, H. Westphal. Laboratory of Mammalian Genes and Development, NICHD, National Institutes of Health, Bethesda, MD; Developmental Skin Biology Unit, NIAMS, National Institutes of Health, Bethesda, MD; Laboratory of Mammalian Genes and Development, NICHD, NIH, MD
293 B213 Function of Bhlhb5 (Beta3) transcription factor in retinal development. L. Gan, L. Feng. University of Rochester, Rochester, NY 14642
294 B214 Pax6 function in the vertebrate retina. Y. Hsieh, X. Zhang, J. Horwitz, X. Yang. Molecular Biology Institute and Jules Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles
295 B215 Math5 is required for early retinal neuron differentiation and cell cycle progression. N.L. Brown, E. Wroblewski, S. Patel, T.T. Le. Children’s Hospital Research Foundation, Cincinnati, OH; Childrens Memorial Institute for Education and Research, Chicago, IL
296 B216 Frizzled 5 signaling governs the neural potential of progenitors in the developing Xenopus retina. K.B. Moore, T.J. Van Raay, I. Iordanova, M. Steele, M. Jamrich, W. Harris, M.L. Vetter. University of Utah, Salt Lake City, UT 84054; Vanderbilt University, Nashville, TN 37235; University of Cambridge, Cambridge, UK; Baylor College of Medicine, Houston, TX 77030
297 B217 Analysis of Pax6 alpha transcripts using BAC technology reveals restricted expression in amacrine cells. J. Lakowski, J.D. Lauderdale. University of Georgia, Athens, GA 30602
298 B218 Math5 establishes ganglion cell competence in postmitotic retinal cells. T. Glaser, J.A. Brzezinski. University of Michigan, Ann Arbor, MI 48109
299 B219 DLX1 and DLX2 regulation of the neurotrophin receptor Trk-B and survival of late-born retinal ganglion cells in the vertebrate retina. J. de Melo, Q. Zhou, S. Cheng, D. Eisenstat. Depts. of Anatomy and Pediatrics, University of Manitoba, Winnipeg, Canada
300 B220 Wnt-3a regulation of somite myogenesis. M. Baranski, S. Hauschka. University of Washington, Seattle, WA 98109
301 B221 Potency of skeletal muscle stem cells in the early chick embryo. M. George Weinstein, J. Gerhart, C. Neely, L. Narciso, J. Elder, T. Kvist, K. Knudsen, Philadelphia College of Osteopatic Medicine, Philadelphia, PA 19131; Lankenau Institute for Medical Research, Wynnewood, PA 19096 558 Developmental Biology Volume 283, 2005
302 B222 MyoD positive cells of the pregastrulating embryo are critical for morphogenesis and skeletal muscle differentiation. J. Elder, J.V. Gerhart, C. Neely, H. Eisenbrock, T. Kvist, K. Knudsen, M. George-Weinstein. Philadelphia College of Osteopathic Medicine, Philadelphia, PA 19131; Lankenau Institute for Medical Research, Wynnewood, PA 19096
303 B223 MyoD positive cells of the pregastrulating embryo are committed to the skeletal muscle lineage. L. Narciso, J. Gerhart, J. Perlman, C. Neely, K. Linask, M. George-Weinstein. Philadelphia College of Osteopathic Medicine, Philadelphia, PA 19131; University of South Florida, St. Petersburg, FL 33701
304 B224 Muscle specification in Xenopus laevis gastrula-stage embryos. S.A. Rizvi, K. Wunderlich, J.K. Gustin, C.R. Domingo. San Francisco State University, San Francisco, CA 94132; Biomolecular Resource Center, UCSF, San Francisco, CA 94143; Oregon Health Sciences University, Portland, OR 97201
305 B225 Hedgehog signaling negatively regulates pax3/7 expression in presumptive myogenic precursors in zebrafish. X. Feng, E.G. Adiarte, S.H. Devoto. Biology Department, Wesleyan University, Middletown, CT 06459
306 B226 Identification of a myogenic precursor population underlying growth in zebrafish. F. Stellabotte, X. Feng, S.E. Patterson, D.A. Fernandez, S.H. Devoto. Wesleyan University, Department of Biology, Middletown, CT 06459
307 B227 Zebrafish Mibta52b antagonizes Miblike in neurogenesis and compromises DeltaD in somitogenesis. C. Zhang, Q. Li, C. Lim, Y-J. Jiang. Institute of Molecular and Cell Biology, Singapore 138673
308 B228 fbf-1 and fbf-2: nearly identical genes with unique roles in C. elegans germline cell fate determination. L.B. Lamont, J. Kimble. University of Wisconsin-Madison; University of Wisconsin-Madison and HHMI, Madison, WI 53706
309 B229 Fgf9 signaling is required for Sertoli precursor cells to establish the male pathway. Y. Kim, A. Kobayashi, R. Sekido, B. Capel, R.R. Behringer, R. Lovell-Badge. Dept. of Cell Biology, Duke University Medical Center, Durham, NC; Dept. of Molecular Genetics, University of Texas, MD Anderson Cancer Center, Houston, TX 77030; Div. of Developmental Genetics, MRC National Institute for Medical Research, London NW7 1AA, UK
310 B230 Competence windows for endocrine differentiation in the developing mouse pancreas. K.A. Johansson, U. Dursun, N. Jordan, G. Gradwohl, A. Grapin-Botton. ISREC, 1066 Epalinges, Switzerland; INSERM unit 381, 67200 Strasbourg, France
311 B231 Regulation of pancreatic islet cell differentiation. L. Sussel, C. Prado, C. Chao. University of Colorado Health Sciences Center, Aurora, CO 80045
312 B232 Wnt h-catenin signaling induces synovial joint formation and specifies osteoblast and chondrocyte cell fates. Y. Yang, T.F. Day, X. Guo, L. Garret-Beal. Genetic Disease Research Branch, NHGRI, NIH
313 B233 Transcription factors coordinately expressed during adult, murine T-lymphocyte specification. E. David, C.C. Tydell, M.K. Anderson, G. Buzi, T. Taghon, L. Rowen, E.V. Rothenberg. California Institute of Technology, Pasadena, CA 91125; University of Toronto, Canada M4N 3M5; Institute for Systems Biology, Seattle, WA
Stem Cells and Tissue Regeneration
314 B234 Cellular assessment of calcium aluminate materials for bone tissue engineering. J.S. Doctor, J.T. Leung, K.A. McGowan. 1. Department of Biological Sciences, Duquesne University, Pittsburgh, PA 15282; 2. Westmoreland Advanced Materials, Arnold, PA 15068
315 B235 Expression profiles of genetically identical mesenchymal stem cells indicate diversity of multipotent cell populations. K. Kusumi, V. Markov, D.A. William, V. Lounev, D.M. Hall, E.F. Rappaport, R.I. Cohen, B. Saitta. The Children’s Hospital of Philadelphia and University of Pennsylvania School of Medicine, Philadelphia, PA 19104; The Coriell Institute for Medical Research, Camden, NJ 08103
316 B236 Generation of peripheral sensory and sympathetic neurons and neural crest cells from human embryonic stem cells. R.S. Goldstein, O. Pomp, R.E. B, I. Brukhman. Bar-Ilan University, Ramat-Gan, 52900, Israel; Human Embryonic Stem Cell Research Center, Jerusalem, 91120 Israel
317 B237 Epigenetic reprogramming of somatic nuclei with human embryonic stem cells. K.C. Eggan, C.A. Cowan, B. Haas. Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138
318 B238 Primitive endoderm-like cells derived from human embryonic stem cells support maintenance of pluripotency. R. Gonzalez, X. Parsons, T. McDaniel, S. Baker, S. Oeser, D. Barker, E.Y. Snyder, J.F. Loring. Burnham Institute, La Jolla, CA 92037; Ilumina Inc., San Diego, CA 92021
319 B239 Appositional bone growth and digit regeneration in mice and humans. M. Han, X. Yang, J. Lee, C. Brownyard, S. Escudero, K. Johnson, C. Allan, K. Muneoka. Tulane University, New Orleans, LA 70118; University of Washington, School of Medicine, Seattle, WA 98104 SDB Meeting Abstracts 559
320 B240 Withdrawn
321 B241 Wnt signaling and lineage segregation in mouse embryonic stem cells. N. Slawny, T. Gratsch, S. O’Shea. Department of Cell and Developmental Biology, University of Michigan
322 B242 Ciliated cells serve as progenitor during regeneration of the respiratory epithelium. K. Park, J.M. Wells, A.M. Zorn, S.E. Wert, V.E. Laubach, L.G. Fernandez, J.A. Whitsett. Division of Pulmonary Biology; Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center and the University of Cincinnati College of Medicine, Cincinnati, OH 45229; Department of Surgery, University of Virginia Health System, Charlottesville, VA
323 B243 Fibroblast growth factor 8: A crucial signaling molecule for olfactory epithelium neurogenesis. R. Santos, S. Kawauchi, A.L. Calof. University of California, Irvine
324 B244 Recapitulation of in vivo gene expression during hepatic differentiation from mouse embryonic stem cells. Y. Yamamoto, T. Teratani, G. Quinn, T. Kato, T. Ochiya. Sec. Meta., National Cancer Center Research Institute, Tokyo, Japan; Waseda University, Tokyo, Japan
325 B245 Evolutionary filtering for genes in the Oct4 (Pou5F1) pathway. S.M. Burgess, Z. Wang, L. Garrett-Beals, A. Elkahloun, Y. Chen. National Human Genome Research Institute/National institutes of Health
326 B246 Expression of immune-related genes during limb regeneration in Xenopus laevis. M.W. King, L. Abu-Niaaj, A.L. Mescher, E.A. Moseman, A.W. Neff. IU School of Medicine, THC, Terre Haute, IN 47809; IU School of Medicine, MSP, Bloomington, IN 47405
327 B247 Characterization of tgf-h1 expression during wound healing and limb regeneration in the axolotl. M. Le´vesque, M. Pilote, S. Roy. Department of Biochemistry; Department of Stomatology, Universite´ de Montre´al, Montre´al QC H3T 1J4 Canada
328 B248 A proximodistal gradient of Fgf signaling defines position-dependent rates of zebrafish fin regeneration. Y. Lee, S. Grill, M. Murphy-Ryan, K.D. Poss. Duke University Medical Center, Durham, NC 27710
329 B249 Inhibition of bmp signaling during zebrafish fin regeneration disrupts fin growth and bone matrix deposition. A. Smith, F. Avaron, B. Padhi, M. Akimenko. Ottawa Health Research Institute, University of Ottawa, Canada
330 B250 Pax7 in adult and regenerated tails of the teleost S. macrurus. C. Weber, G.A. Unguez. NMSU, Las Cruces, NM 88003
331 B251 Spherule cells and their role in the intestinal regeneration of Holothuria glaberrima. J.E. Garcı´a-Arrara´s, C. Schenk, R. Rodrı´guez-Ramı´rez, I.I. Torres, A. Cabrera-Serrano, A.G. Candelaria, S. File, G. Valentı´n-Tirado. University of Puerto Rico, Rio Piedras, PR 00931
332 B252 The totipotent stem cell systems of the flatworm Macrostomum lignano and the acoel Convoluta pulchra. P. Ladurner, D. Pfister, R. Gschwentner, K. Nimeth, B. Egger, R. Rieger. University of Innsbruck, Austria
333 B253 The totipotent stem cell system as a source for germ line cells during development and regeneration in the flatworm Macrostomum lignano. D. Pfister, P. Ladurner. Institute of Zoology, University of Innsbruck, Austria
334 B254 Germ cell lineage behaves differently from somatic stem cells during the regeneration of Enchytraeus japonensis, an oligochaete worm that undergoes both asexual and sexual reproduction. R. Tadokoro, M. Sugio, J. Kutsuna, S. Tochinai, Y. Takahashi. NAIST, Nara, Japan; Hokkaido University, Sapporo, Japan; CDB, RIKEN, Kobe, Japan
335 B255 Silencing of the CREB Pathway Prevents Hydra Head-Regeneration Through Inhibition of Apoptosis in Regenerating Tips. B. Galliot, L. Ghila, K. Dobretz, S. Chera. Dept. of Zoology and Animal Biology, University of Geneva, CH 1211-Geneva 4, Switzerland
336 B256 Stem cell-specific gene expression in leech. D.H. Shain, K.A. Hohenstein. Rutgers, The State University of New Jersey, Camden, NJ 08102
337 B257 A planarian Etr-1 homolog is required for stem cell maintenance. T. Guo, P.A. Newmark. University of Illinois at Urbana-Champaign, IL 61801
338 B258 Chromatin architecture in the planarian Schmidtea mediterranea. S.M. Robb, A. Sa´nchez Alvarado. University of Utah School of Medicine, Department of Neurobiology and Anatomy
339 B259 Identification of stem cell gene candidates in the planarian Schmidtea mediterranea. G.T. Eisenhoffer, A. Sa´nchez Alvarado. University of Utah School of Medicine
340 B260 Cell cycle dynamics of a stem cell population in vivo: the neoblasts of the planarian Schmidtea mediterranea. H. Kang, A. Sa´nchez Alvarado. University of Utah School of Medicine
341 B261 Dissecting the molecular basis of neural regeneration in planarians. R.M. Zayas, F. Cebria`, P.A. Newmark. Department of Cell and Structural Biology, Neuroscience Program, University of Illinois at Urbana-Champaign, IL, USA 560 Developmental Biology Volume 283, 2005
Germ Cells and Gametogenesis
342 B262 Development of viable acetabularia acetabulum after cryopreservation of gametangia. D.F. Mandoli, K. Wherry, A. Murakami-Sekimata. University of Washington, Seattle, WA 98195-5325; University of Washington, Seattle, WA 98195-5325
343 B263 The indeterminate gametophyte1 gene of maize encodes a member of the Lateral Organ Boundaries domain protein family. M.M. Evans. Department of Plant Biology, Carnegie Institution, Stanford, CA 94305
344 B264 Functional genomic analysis of germ cell specification in planarians. J.M. Stary, Y. Wang, R.M. Zayas, G.T. Eisenhoffer, A. Sa´nchez Alvarado. Dept. of Cell and Structural Biology, Neuroscience Program, University of Illinois at Urbana-Champaign; Dept. of Neurobiology and Anatomy, University of Utah School of Medicine
345 B265 Early development of polarized oogenic cysts in the polychaete worm, Ophryotrocha labronica. J.L. Brubacher, E. Huebner. Dept. of Zoology, University of Manitoba, Winnipeg, MB, Canada
346 B266 C. elegans germline mitotic region possesses germline stem cells. S.L. Crittenden, K. Leonhard, D. Byrd, M. Lee, J. Kimble. University of Wisconsin-Madison and HHMI, Madison, WI 53706
347 B267 Dangerous liaisons: the Caenorhabditis kinase KGB-1 targets GLH-1; both associate with the COP9 signalosome subunit CSN-5. K. Bennett, W. Li, A. Orsborn. MMI Dept., University of Missouri
348 B268 Transcription regulation of endogenous genes by C. elegans RNA Helicase A. K.M. Walstrom, C. Bauer, D. Schmidt. New College of Florida, Division of Natural Sciences, Sarasota, FL 34243
349 B269 Identifying GLD-2 target mRNAs in C. elegans. N. Suh, J. Kimble. Department of Biochemistry; Departments of Biochemistry, Genetics, and Molecular Biology, University of Wisconsin-Madison, and Howard Hughes Medical Institute, 433 Babcock Drive, Madison, WI 53706, USA
350 B270 Loss of Pan-1 causes a Peter Pan-like phenotype in C. elegans. G. Gao, K. Bennett. University of Missouri-Columbia
351 B271 A genetic screen to identify the targets of puf-8, a Puf family gene involved in the meiotic progression of C. elegans spermatocytes. K. Subramaniam, M. Ariz. Dept. of Biol. Sci. and Bioeng., Indian Institute of Technology, Kanpur 208016, India
352 B272 Identification and analysis of new germline sex specific genes. A.L. Casper, M. Van Doren. Department of Biology, Johns Hopkins University, Baltimore, MD 21218
353 B273 Characterization of genes required for germ cell development in Drosophila melanogaster. J.D. Bodensteiner, C.R. Coffman. Iowa State University, Ames, IA 50014
354 B274 Germ cell migration and death: Characterization of scattershot and ousiders expression in Drosophila melanogaster. K.D. Andersen, C.R. Coffman. Iowa State University, Ames, IA 50011
355 B275 Disruption of germ cell migration and programmed cell death in Drosophila embryogenesis: the scattershot mutant. A.R. Kamps, C.R. Coffman. Iowa State University, Ames, IA 50011
356 B276 The outsiders gene is necessary for the programmed cell death of primordial germ cells during Drosophila embryonic development. Y. Yamada, C.R. Coffman. Iowa State University, Ames, IA 50010
357 B277 Vreteno, a new gene required for germ line stem cell differentiation in Drosophila. M.Y. Davis, E. Staeva-Vieira, R. Lehmann. Skirball Institute/HHMI, New York University School of Medicine, NY 10016
358 B278 Direct control of germline stem cell division and cyst growth by neural insulin in Drosophila. D. Drummond-Barbosa, L. LaFever. Vanderbilt University Medical Center, Nashville, TN 37232-8240
359 B279 Zebrafish Staufen in germ cell specification. S. Ramasamy, W. Hui, K. Sampath. Laboratory of Vertebrate Development, 1, Research Link, Temasek Life Sciences Laboratory, National University of Singapore, Singapore-117604
360 B280 The RNA binding protein Hermes, may have several important functions during early development of Xenopus laevis. H. Song, L.D. Etkin. University of Texas-M.D. Anderson Cancer Center, Graduate school of Biomedical Sciences Program in Genes and Development, Houston, TX
361 B281 FGF9 is required for male germ cell survival. L. DiNapoli, B. Capel. Duke University – Dept. of Cell Biology – Durham, NC 27710
362 B282 The role of the forkhead gene Foxc1 in gonad development. D.M. Mattiske, B. Hogan. Duke University Medical Center, NC 27710
363 B283 Oocyte-specific MATER affects microtubule formation and may suppress protein secretion. M. Ohsugi, S. Hess, J. Dean. NIDDK, NIH, Bethesda, MD 20892 SDB Meeting Abstracts 561
Poster Session II Pacific Concourse Friday, July 29, 3–7 pm (set-up), 9–11 pm Saturday, July 30, 12:30–3 pm, 9–11 pm Sunday, July 31, 12:30–3 pm (tear down at end of session) Numbers in italics indicate Program Abstract numbers. ‘‘B’’ numbers indicate Poster Board numbers. Odd number board authors present on Friday, July 29, 9–11 pm Even number board authors present on Saturday, July 30, 9–11 pm
Development and Evolution
364 B1 Understanding the functional evolution of trunk Hox genes in arthropods. C.C. Hsia, W. McGinnis. University of California, San Diego
365 B2 The role of Hox genes in crustacean appendage specialization. D.M. Liubicich, J.M. Serano, N.H. Patel. University of California, Berkeley/ HHMI, CA 94720
366 B3 Hox gene expression initiates in single-cell wide parasegment precursors in the amphipod crustacean Parhyale hawaiensis. J. Serano, D.M. Liubicich, W.E. Browne, N.H. Patel. University of California, Berkeley/HHMI, CA 94720; University of Hawaii
367 B4 Evolution of Hox gene expression and function in mammals. C. Chen, C.J. Cretekos, J.J. Rasweiler IV, G.B. Adebayo, R.R. Behringer. University of Texas MD Anderson Cancer Center, Houston; SUNY Downstate Medical Center, Brooklyn
368 B5 Evolution of gastrulation gene networks in the honeybee Apis mellifera. J.D. Cande, M. Levine. University of California Berkeley
369 B6 Nodal signaling and the evolution of deuterostome gastrulation. H.K. Chea, B.J. Swalla. Friday Harbor Laboratories, University of Washington, Seattle, WA 98195
370 B7 Embryonic TGF-beta signaling in cnidaria and higher animals: Similar genes – similar functions? G.H. Thomsen, D. Matus, K. Pang, T. Kalkan, M.Q. Martindale. Stony Brook University, Stony Brook, NY; University of Hawaii, Kewalo Marine Lab, Honolulu, HI
371 B8 Melanocyte development of the invertebrate chordate Ciona intestinalis. A.C. Cone, R.W. Zeller. San Diego State University
372 B9 Evidence for complex evolutionary origins of a sea urchin polyketide synthase gene. C. Calestani, T.A. Castoe, T. Stephens, C.L. Parkinson. University of Central Florida, Orlando, FL 32816
373 B10 Gene expression analysis of germ layer evolution in the indirectly developing polychaete annelid Hydroides elegans. C. Arenas-Mena. San Diego State University
374 B11 The GATA family of receptors within the polychaete Platyneries dumerilii. W.J. Gillis, B.A. Bowerman, S.Q. Schneider. Institute of Molecular Biology, University of Oregon, Eugene, OR 97403-1229
375 B12 Molecular composition of epithelial cell junctions in the polychaete Platynereis dumerilii. S.Q. Schneider, B.A. Bowerman, C.Q. Doe. University of Oregon, Eugene, OR 97403
376 B13 Cocoon production and function in leech development. D.H. Shain, C.W. Sayers, J. Coleman, C. Dimitriu, T.A. Mason. Rutgers, The State University of New Jersey, Camden, NJ 08102
377 B14 Diverse functions of Wnt signaling during nematode vulva formation: A comparison between Pristionchus pacificus and C. elegans. M. Zheng, H. Xiao, R.J. Sommer. Max-Planck Institute for Developmental Biology, Tuebingen 72076, Germany
378 B15 Dorsoventral patterning in a short germ insect. M. van der Zee, S. Roth. University of Cologne, 50931 Cologne, Germany
379 B16 Waves of runt expression segment the short germ insect Tribolium castaneum. S.C. Rhoades, S.J. Brown. Kansas State University, Manhattan, KS 66506
380 B17 Netrins: Creating divergent morphologies. M. Duman-Scheel, S. Clark, E. Grunow, W. Simanton, A. Hasley, B. Hill. Albion College, Albion, MI 49224
381 B18 Direct regulation of knot by Ultrabithorax and the evolution of cis-regulatory elements in Drosophila. B.M. Hersh, S. Carroll. University of Wisconsin-Madison, HHMI
382 B19 Anterior patterning in the lower cyclorrhaphan flies Megaselia and Episyrphus. S.J. Lemke, A. Rafiqi, S. Ferguson, U. Schmidt-Ott. Department of Organismal Biology and Anatomy, The University of Chicago, Chicago, IL 60637
383 B20 The role of the pupal specifier, Br in the direct-developing milkweed bug, Oncopeltus fasciatus. D.F. Erezyilmaz, L.M. Riddiford, J.W. Truman. University of Washington, Seattle, WA 98195-1800 562 Developmental Biology Volume 283, 2005
384 B21 Notch expression in Schistocerca americana. B.J. Blachuta, K.M. Curry, L.M. Nagy. University of Arizona, Tucson, AZ 85716
385 B22 Notch signaling during segmentation of the amphipod crustacean, Parhyale hawaiensis. K.E. O’Day, N.H. Patel. UC Berkeley and HHMI
386 B23 Segmentation in branchiopod crustaceans. A.M. VanHook, N.H. Patel. Howard Hughes Medical Institute and University of California at Berkeley
387 B24 Characterization of pair-rule orthologs from the crustacean, Parhyale hawaiensis. R.J. Parchem, W.E. Browne, M. Gerberding, C.C. Babbitt, N.H. Patel. U. of California, Berkeley/HHMI; Kewalo Marine Lab., U. of Hawaii; Max-Planck-Institut fu¨r Entwicklungsbiologie-Tu¨bingen
388 B25 Expression of appendage patterning genes in the crustacean Parhyale hawaiensis. K.D. Tran, N.H. Patel. University of California, Berkeley/ HHMI, CA 94720
389 B26 Gene expression from DNA injected into embryos of the amphipod crustacean Parhyale hawaiensis. E. Rehm, N.H. Patel. Howard Hughes Medical Institute, University of California-Berkeley
390 B27 The role of ectodermal stem cells in Porcellio scaber. M.A. Vargas-Vila, N.H. Patel. University of California, Berkeley/HHMI
391 B28 Hedgehog signaling is required at multiple stages of zebrafish tooth development. W.R. Jackman, D.W. Stock. University of Colorado, Boulder, CO, USA
392 B29 Expression of a presumptive Pax1 ortholog in Xenopus laevis: Insights into the evolution and development of the anuran axial skeleton. G.R. Handrigan, R.J. Wassersug. Dalhousie University, Halifax, Canada
393 B30 Xenopus tropicalis ParaHox genes: conserved roles in posterior development and neurogenesis. H.V. Isaacs, J.C. Illes, L. Faas. University of York, York, YO10 5YW, United Kingdom
394 B31 Cloning, expression and evolution of skeletogenic genes (BMP-2/noggin) from the turtle Trachemys scripta. E.E. LeClair, C.A. Bien, F.A. Merchant. DePaul University, Chicago, IL 60614
395 B32 Gene expression patterns in the development of the skeletogenic mesenchyme in the red-eared slider turtle, Trachemys scripta. J.E. Moustakas. University of California, Berkeley
396 B33 The role of FGF signaling in the formation of the turtle carapace. J.A. Cebra-Thomas, P.N. Riccio, J.C. Simonet, S.F. Gilbert. Swarthmore College, Swarthmore, PA 19081
397 B34 Plasticity and constraint during cranial skeletogenesis. B.F. Eames, R.A. Schneider. Orthopaedic Surgery, UCSF, San Francisco, CA 94143
398 B35 Origins of the cardiac neural crest. M. Ezin, M. Bronner-Fraser. California Institute of Technology
399 B36 Casein Kinase I phosphorylates the forkhead transcription factor FoxG1 and promotes its nuclear localisation. T. Regad, N. Papalopulu. The Wellcome Trust/Cancer research UK Gurdon Institute, Tennis court Road, Cambridge, CB2 1QR United Kingdom; Department of Anatomy, Downing site, University of Cambridge, United Kingdom
400 B37 Cilia of the early rabbit embryo point to non-conserved modes for breaking symmetry in the mammalian embryo. A. Fischer, K. Muders, O. Selchow, H.W. van Straaten, C. Viebahn, M. Blum. University of Hohenheim, 70593 Stuttgart, Germany; University of Stuttgart, 70569 Stuttgart, Germany; University of Maastricht, Maastricht, The Netherlands; University of Go¨ttingen, 37075 Go¨ttingen, Germany
401 B38 BMP signals control interdigital programmed cell death by regulating FGF signaling. S.P. Underwood, C. Wilson, Y. Mishina, M. Lewandoski. CCR, NCI-Frederick/NIH; NIES/NIH
402 B39 Loss of the alpha7 integrin affects vascular development and integrity. J.V. Welser, N.L. Flintoff-Dye, D.J. Burkin. University of Nevada, Reno, NV 89557
403 B40 Purified Wnt proteins control cell proliferation and differentiation during chondrogenesis in limb bud mesenchyme cultures. D. ten Berge, R. Nusse. Howard Hughes Medical Institute, Stanford University Medical School, Stanford, CA 94305
Patterning and Transcription Factors
404 B41 Drawing a line in the Arabidopsis fruit. A. Roeder, S. Liljegren, C. Ferra´ndiz, M. Yanofsky. UCSD, 9500 Gilman Dr. DEPT 0116, La Jolla, CA 92093, USA; Dept. of Biology, UNC at Chapel Hill, Chapel Hill, NC 27599, USA; Instituto de Biologia Molecular y Celular de Plantas, Av de los Naranjos s/n, Valencia 46022, Spain
405 B42 Leaf polarity and growth factors play important roles in patterning the fruit in Arabidopsis. J.R. Dinneny, D. Weigel, M.F. Yanofsky. University of California, San Diego, La Jolla, CA 92093-0116; Max Planck Institute for Dev. Biol., Tu¨bingen D-72076, Germany SDB Meeting Abstracts 563
406 B43 Mesp separately regulates heart specification and migration in the Ciona tadpole. B.J. Davidson, W. Shi, M.S. Levine. University of California, Berkeley
407 B44 Understanding expression noise in pattern formation with the Drosophila segmentation network. D.M. Holloway, C.E. Vanario-Alonso, J. Reinitz, A.V. Spirov. British Columbia Institute of Tech., Burnaby, Canada; SUNY Stony Brook, NY 11794, USA
408 B45 The EGF receptor affects selector gene expression through wingless, and affects cell affinity during Drosophila eye-antennal imaginal disc patterning. J.R. Curtiss, M. Mlodzik. Mount Sinai School of Medicine, New York, NY 10029
409 B46 Using salivary glands as a model system to study the cell biology of Hh signaling in Drosophila. B.E. Sisson, M.L. Killingsworth, K.K. McCabe, R.A. Holmgren. Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL
410 B47 Regulation of Cubitus interruptus by a conserved domain. S.L. Ziegenhorn, J.A. Croker, R.A. Holmgren. Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL
411 B48 A novel conserved gene, paladin, is dynamically expressed in the midline and paraxial mesoderm of zebrafish embryos. X. Xu, T.M. Han, S.L. Amacher. University of California, Berkeley
412 B49 Characterization of the roles of two Suppressor of Hairless genes during zebrafish somitogenesis. K. Echeverri, A.C. Oates. Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
413 B50 Zebrafish foxd3 is selectively required for neural crest sublineage determination, migration and survival. R.A. Stewart, B.L. Arduini, C.A. Jette, J.P. Kanki, P.D. Henion, A. Look. Dept. of Pediatric Oncology, Dana-Farber Cancer Institute, 44 Binney Street, Boston, MA; Dept. of Neuroscience, Ohio State University, Columbus, OH
414 B51 AP-2 transcription factors function at multiple steps of zebrafish neural crest. W. Li, R.A. Cornell. University of Iowa, Iowa City, IA 52242
415 B52 Molecular mechanisms specifying separate hindbrain and spinal cord regions from the neural plate. I. Skromne, D. Thorsen, M.E. Hale, V. Prince, R.K. Ho. Dept. Organismal Biology and Anatomy, University of Chicago, Chicago, IL 60637, USA
416 B53 Insertional mutagenesis in zebrafish identifies novel genes involved in forebrain development. E.C. Swindell, A. Amsterdam, N. Hopkins, M. Jamrich. Baylor College of Medicine, Houston, TX; Massachusetts Institute of Technology, Cambridge, MA
417 B54 Nlz, a NET zinc finger protein has repressor function during zebrafish hindbrain development. M.O. Nakamura, A.P. Runko, C.G. Sagerstrom. University of Massachusetts Medical School, Worcester, MA 01605
418 B55 Zebrafish Lmx1b.1 and Lmx1b.2 are necessary to maintain the Isthmic Organizer. P. O’Hara, L.K. Barr, D.S. Kessler. University of Pennsylvania, Philadelphia, PA 19104; Harvard Medical School, Boston, MA 02115
419 B56 Genetic pathways regulating rhombomere 4/5 formation. C. Sagerstrom, S. Choe, N. Hirsch. University of Massachusetts Medical School, Worcester, MA
420 B57 Endodermal patterning in zebrafish: pancreas development. K.M. Alexa, P. diIorio, L. Etheridge, C. Sagerstro¨m. University of Massachusetts Medical School, Worcester, MA 01605
421 B58 Activation of goosecoid transcription by siamois and twin. C.D. Reid, S. Bae, D.S. Kessler. University of Pennsylvania, Philadelphia, PA 19104
422 B59 A role for Notch signaling in mesoderm patterning of Xenopus laevis. C.M. Gaydos, K.A. McLaughlin. Department of Biology, Tufts University, Medford, MA 02155
423 B60 A genomic screen for zic1 target genes in early neural development. S. Li, K.W. Cho, C.S. Merzdorf. Department of Cell Biology and Neuroscience, Montana State University, Bozeman; Department of Developmental and Cell Biology, University of California, Irvine
424 B61 Circadian genes are expressed during early development in Xenopus laevis. K. Curran, S. LaRue, C. Green. University of Wisconsin- Whitewater; University of Virginia, Charlottesville, VA 22904
425 B62 Nkx6 genes in patterning of frog neural plate. D.S. Dichmann, R.M. Harland. University of California, Berkeley, 571 Life Sciences Addition, Berkeley, CA 94720
426 B63 Six3 functions in anterior neural plate specification by promoting cell proliferation and inhibiting BMP4 expression. M. Andreazzoli, G. Gestri, M. Carl, I. Appolloni, S.W. Wilson, G. Barsacchi. Laboratori di Biologia Cellulare e dello Sviluppo, Universita’ di Pisa, Via Carducci 13, 56010 Ghezzano (Pisa), Italy; Department of Anatomy and Developmental Biology, University College of London, Gower Street, London WC1E 6BT
427 B64 Regulation of Sox Group E activity during neural crest development. K. Taylor, C. LaBonne. Dept. Biochemistry, Molecular Biology, and Cell Biology Northwestern University, Evanston IL 60208 564 Developmental Biology Volume 283, 2005
428 B65 The avian lateroseptal organ and the molecular striatopallidal border. S. Bardet, I. Cobos, E. Puelles, M. Martı´nez-de-la-Torre, L. Puelles. University Murcia Med. School, E30071 Murcia, Spain; University California San Francisco, Med. School, N. Ireland Lab. Devel. Neurobiol., SF 94143; University Miguel Herna´ndez, Institute Neurociencias-CSIC, E3550 San Juan, Alicante, Spain
429 B66 Regulation of motor neuron progenitor differentiation by a network of HLH proteins. B.G. Novitch, S.E. Weicksel, T.M. Jessell. University of Michigan Medical School, Ann Arbor, MI; HHMI, Columbia University, New York, NY
430 B67 Tbx5 transcriptional activity is mediated by sub-cellular localization. T. Camarata, H. Simon. Northwestern University, Feinberg School of Medicine, Children’s Memorial Research Center, Chicago, IL 60614
431 B68 Hedgehog signaling, intraflagellar transport protein and skeletal development. B. Song, B.K. Yoder, R. Serra. University of Alabama at Birmingham
432 B69 The role of cilia/IFT in Shh signaling and limb patterning. C.J. Haycraft, Q. Zhang, B. Banizs, B.K. Yoder. Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294
433 B70 Establishment of an Lmx1b dorsal-ventral sharp boundary is coordinately linked to AER formation during mouse limb development. Q. Qiu, R.L. Johnson. University of Texas, M.D. Anderson Cancer Center, Houston, TX 77030
434 B71 Temporal requirements of Sonic Hedgehog expression for specifying different digits. J. Zhu, M. Nguyen, S. Mackem. Laboratory of Pathology, Center for Cancer Research, NCI, NIH, Bethesda, MD 20892, USA
435 B72 The role of Sox6 in muscle development. N. Hagiwara, B. Ma, A. Ly, Q. Nguyen, M. Yeh. University of California, Davis, CA 95616
436 B73 Identification of Foxl1 target genes in the mesenchyme of the gastrointestinal tract. S.D. Sackett, K.H. Kaestner. University of Pennsylvania, Philadelphia, PA 19104
437 B74 Understanding the role of Gata transcription factors in the developing mouse pancreas. K.J. Decker, L. Sussel. University of Colorado Health Sciences Center, Aurora, CO 80045
438 B75 Analysis of Baf250 and the functional distinction between mammalian Swi/Snf complexes. J. Brennan, T. Magnuson. University of North Carolina-Chapel Hill
439 B76 Phenotypic recovery of TGFbeta2 null embryos by downregulated Pax3. C.K. Mayanil, H. Nakazaki, B.A. Stahl, B. Mania-Farnell, D. George, E.G. Bremer, D.G. McLone. CMRC and Northwestern University Feinberg School of Medicine, Chicago, IL 610614; Purdue University Hammond, IN 46343
440 B77 The transcriptional repressor Snai1 is required for left-right asymmetry determination in the mouse. S.A. Murray, E.A. Carver, K.F. Oram, T. Gridley. The Jackson Laboratory, Bar Harbor, ME 04609
441 B78 Elucidating the timing of the endothelin—A receptor function during craniofacial development. L. Ruest, T. Brock, D.E. Clouthier. Department of Molecular, Cellular and Craniofacial Biology, University of Louisville, Louisville, KY 40292; Encysive Pharmaceuticals, Houston, TX 77030
442 B79 Role of the PDGFRs on the migratory ability of cardiac neural crest cells. A.M. Richarte, M.D. Tallquist. UT Southwestern Medical Center
443 B80 Tbx4 regulates endothelial cell fate decisions in the allantois. V.E. Papaioannou, L.A. Naiche. Dept. of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
444 B81 Analysis of eye development in mice with a targeted mutation of the Tbx2 gene. H. Behesti, J.C. Sowden, V.E. Papaioannou. Developmental Biology Unit, Institute of Child Health, UCL, London; Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, NYC
445 B82 Signaling downstream of Pitx2 is required for normal development of the optic stalk. A.L. Evans, P.J. Gage. University of Michigan, Ann Arbor, MI 48105
446 B83 Identification of Gli target genes using chromatin immunoprecipitation with a genetically inducible system on genomic arrays. S.A. Vokes, H. Ji, W.H. Wong, A.P. McMahon. Harvard University, Cambridge, MA 02138; Stanford University, Palo Alto, CA 94305
447 B84 Multiple roles of Gli3 in mouse inner ear patterning. J. Bok, D.K. Wu. National Institute on Deafness and Other Communication Disorders, Rockville, MD 20850
448 B85 A requirement for Gbx1 in normal locomotion. S.T. Waters, C. Wilson, M. Lewandoski. Cancer and Developmental Biology Laboratory, National Cancer Institute-Frederick, National Institute of Health, Frederick, MD 21702 SDB Meeting Abstracts 565
449 B86 A role for the vHNF1 homeodomain protein in establishing early rhombomere identity by direct regulation of Kreisler expression. S.P. Cordes, F.A. Kim, A. Sing, T. Kaneko, M. Biemann, N. Stallwood, V.S. Sadl. Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, Toronto, ON, Canada M5G 1X5; Department of Medical Genetics and Microbiology, University of Toronto, Toronto, ON, Canada M5G 1X5
450 B87 Role of the Fgf and Wnt genes in forebrain patterning. U. Borello, J.L. Rubenstein. University of California San Francisco, CA 94158
451 B88 Genetic dissection of the eole of En2 during cerebellum development. Y. Cheng, S.K. Sgaier, G. Rocco, M. Villanueva, F. Berenshteyn, A.L. Joyner. HHMI and Skirball Institute, Dept. of Cell Biology, NYUMC
452 B89 The role of the roof plate in cell fate specification and dorsal CNS development. T.E. Borsuk, J.H. Millonig. Graduate School of Biomedical Sciences-UMDNJ; University of Medicine and Dentistry of NJ-RWJMS; CABM, NJ 08854
453 B90 Genome-based approach reveals transcriptional hierarchy regulated by Hoxb1 in the developing central nervous system. G.O. Gaufo, B.R. Arenkiel, M.R. Capecchi. Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249; Department of Neurobiology, Duke University Medical Center, Durham, NC 27710; Department of Human Genetics and Howard Hughes Medical Institute, University of Utah, Salt Lake
454 B91 changing the functional specificity of Hoxa1 in vivo results in multiple homeotic transformations in mouse. N. We´ry, S. Remacle, O. De Backer, N. Pacico, J.J. Picard, R. Rezsohazy. UCL, Louvain-la-Neuve 1348, Belgium; FUNDP, Namur 5000, Belgium
455 B92 Six2 is a downstream target of Hoxa2 in the second branchial arch. E. Kutejova, B. Engist, N. Bobola. Max-Planck-Institute of Immunobiology, 79108 Freiburg, Germany
456 B93 Correlating HOXA13’s DNA binding specificity with loss of function phenotypes. W.M. Knosp, H. Ba¨chinger, H. Stadler. Shriners Hospital for Children Research Division, Portland, OR 97239; Department of Molecular and Medical Genetics, OHSU, Portland, OR 97239; Department of Biochemistry and Molecular Biology, OHSU, Portland, OR 97239
457 B94 Hox11 paralogous genes interact with the Pax-Eya-Six regulatory network in kidney development. D. Wellik, N. Gong, G.R. Dressler, S. Rakshit. University of Michigan Medical Center
458 B95 Osr1 is necessary for metanephric kidney formation. C.N. Kamei, R.G. James, Q. Wang, R. Jiang, T.M. Schultheiss. Molecular Medicine Unit, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston MA 02215; Center for Oral Biology, Department of Biomedical Genetics, University of Rochester School of Medicine and Dentistry, Rochester NY 14642
Organogenesis
459 B96 Role of the Drosophila melanogaster gene bubblegum in nervous system patterning and embryogenesis. A.J. Sivatchenko, A. Schmid, A. Letsou. Department of Human Genetics, University of Utah, Salt Lake City, UT 84112
460 B97 Zed is required for anterior neural tube closure and development of branchial arches in the mouse embryo. J.E. Bloomekatz, K.V. Anderson. Sloan Kettering Institute, 1275 York Ave. New York, NY 10021; Weill Graduate School of Medical Sciences, Cornell University, 445 69th St. New York, NY 10021
461 B98 Noggin plays different roles in mammalian neurulation, depending on axial level. J. Klingensmith, M. Berrong, R. Stottmann. Duke University Medical Center, Durham, NC
462 B99 Regulation of zebrafish brain and visceral left-right asymmetry by brachyury. M.R. Rebagliati, N. Ahmad. University of Iowa, Iowa City, IA 52242
463 B100 The aristaless-related homeobox (Arx) gene product is essential for normal forebrain development. H.M. El-Hodiri, L.E. Kelly. Center for Molecular and Human Genetics, Columbus Children’s Research Institute, Columbus, OH
464 B101 The alien gene is required for normal forebrain development in the mouse. R.W. Stottmann, P. Tran, A. Turbe-Doan, D.R. Beier. Brigham and Women’s Hospital, Boston, MA 02115
465 B102 Retinoic acid is unnecessary for early forebrain development but functions later in the striatum. N. Molotkova, A. Molotkov, G. Duester. The Burnham Institute, La Jolla, CA 92037
466 B103 Generation of neuronal diversity in the chick tectum and the development of tectal-hindbrain projections. N. Fedtsova, E.E. Turner. University of California, San Diego, CA 92093, VA Medical Center, San Diego, CA 92122
467 B104 Induction of functional ectopic eyes in a vertebrate. A.S. Viczian, E. Solessio, M.E. Zuber. SUNY Upstate Medical University, Syracuse, NY 13210
468 B105 Six3 is a key regulator in mammalian eye development. W. Liu, O.V. Lagutin, G. Oliver. Dept. of Genetics, St Jude Children’s Research Hospital, 332 N Lauderdale St, TN 38105 566 Developmental Biology Volume 283, 2005
469 B106 Distinct developmental programs that require different levels of Bmp signaling during mouse retinal development. D. Murali, C. Deng, Y. Furuta. Dept Biochem. and Mol. Biol., University Texas MD Anderson Cancer Center and Program in Genes and Development, Graduate School Biomedical Sciences, University Texas Health Science Center, Houston, TX; National Institute of Diabetes and Digestive and Kidney disease, NIH, Bethesda, MD
470 B107 h-Catenin is required for pattern formation but not cell differentiation in mouse retinal development. X. Fu, H. Sun, W.H. Klein, X. Mu. The University of Texas, M.D. Anderson Cancer Center, Houston, TX 77030
471 B108 Wnt2b/beta-catenin signaling regulates peripheral retinal development. S. Cho, C.L. Cepko. HHMI, Harvard Medical School, Boston, MA 02115
472 B109 Pygopus2 is required for lens induction in mouse. N. Song, L.T. Patterson, L. McClain, K. Schwab, D. Yan, X. Lin, S.S. Potter, R.A. Lang. Division of Dev Biology, Cincinnati Children’s Hospital Research Foundation, Cincinnati, OH; College of Medicine, University of Cincinnati, Cincinnati, OH
473 B110 Wnt dependent regulation of inner ear morphogenesis is balanced by the opposing and supporting roles of Shh. D.J. Epstein, S.M. Takada, M.M. Riccomagno. University of Pennsylvania School of Medicine, Philadelphia, PA 19104; Okazaki Institute for Integrative Biosciences, Okazaki, 444 8787, Japan; University of Buenos Aires School of Medicine, Buenos Aires, Argentina
474 B111 Genetic specification and neural refinement of vestibular function in the zebrafish inner ear. B.B. Riley, S. Kwak, S. Vemaraju, S.J. Moorman. Biology Department, Texas A & M University, College Station, TX 77843-3258; Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Piscataway, NJ 08845-5635
475 B112 BMP signaling in sympathetic preganglionic neuron development. E. Laufer, D. Vasiliauskas, J. Walonoski. Columbia University, New York, NY 10032
476 B113 The role of skeletal muscle-secreted neurotrophic factors in the survival of motor neurons. A.C. Belliveau, B. Kablar. Dalhousie University, Halifax, NS, Canada B3H 4H7
477 B114 Noggin regulation of BMP signaling during pituitary development. S.W. Davis, S.A. Camper. University of Michigan, Ann Arbor, MI 48109-0638
478 B115 Dpp utilizes two distinct mechanisms to specify nonoverlapping subsets of pericardial cells in the Drosophila heart. A.N. Johnson, S.J. Newfeld. Arizona State University, Tempe, AZ 85287
479 B116 Knockdown of plakoglobin in zebrafish development. E.D. Martin, M. Grealy. Pharmacology Department and National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland
480 B117 Genetic and molecular analysis of heart valve formation in zebrafish. T. Bartman, T. Martin. Cincinnati Children’s Hospital Medical Center, Cincinnati, OH 45229
481 B118 A novel component in signaling by a sphingosine 1-phosphate receptor. N. Osborne, N.S. Glickman, J. Alexander, D. Yelon, D.Y. Stainier. UCSF; NYU
482 B119 Molecular anatomy of the embryonic zebrafish heart. G.R. Siegal, I. Adameyko, S. Tevosian, D. Yelon. Skirball Institute, NYU School of Medicine, New York, NY; Dartmouth Medical School, Hanover, NH
483 B120 Genetic analysis of myocardial differentiation and heart morphogenesis in the zebrafish. I.C. Scott, L.A. D’Amico, H. Baier, D.Y. Stainier. University of California, San Francisco
484 B121 Hand2 regulates myocardial differentiation within the lateral plate mesoderm. J.J. Schoenebeck, D. Yelon. Developmental Genetics Program and Department of Cell Biology, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY
485 B122 Hedgehog signaling promotes cardiomyocyte formation in zebrafish. N. Thomas, D. Yelon. Developmental Genetics Program, Skirball Institute, NYU School of Medicine, New York, NY 10010
486 B123 The role of p57kip2 in mouse heart development. S.A. Haley, L.K. Kochilas. Department of Pediatric Cardiology, Rhode Island Hospital, Providence, RI 02903
487 B124 Fgf8 is required for proper anterior heart development. R. Ilagan, R. Abu-Issa, D. Brown, R. Schwartz, E. Meyers. Duke University Medical Center, Durham, NC 27710; University of California-San Francisco 94143; Baylor College of Medicine, Houston, TX 77030
488 B125 A complex nuclear receptor response element controls atrial specificity of the SMyHC3 promoter in mice. T.G. Freitas-Matos, A.C. Sampaio, M. Vasconcelos, T. Sobreira, P. de Oliveira, E. Slonimski, N. Rosenthal, J. Xavier-Neto. InCor-HC.FMUSP, Sa˜o Paulo 05403900, Brazil; EMBL, Monterotondo-Scalo, Italy
489 B126 Tbx2 and Tbx3 display functional overlap during atrioventricular patterning in mouse. Z. Harrelson, V.E. Papaioannou. Columbia University, New York, NY 10032 SDB Meeting Abstracts 567
490 B127 Hedgehog signaling is critical for development of the outflow tract of the heart. M. Goddeeris, E. Meyers. Duke University Med. Ctr., Durham, NC 27710
491 B128 Filamin A is required in endothelial cells for cardiovascular development. Y. Feng, M. Chen, A.M. Mendonza, D.J. Kwiatkowski, C.A. Walsh. HHMI, Dept. of Neurology, BIDMC, Harvard Medical School, Boston, MA 02115; Cardiovascular Division, BWH, Harvard Medical School, Boston, MA 02115; Hematology Division, BWH, Harvard Medical School, Boston, MA 02115
492 B129 A hypomorphic Prep1 mouse shows impairment of angiogenesis and hematopoietic development. E. Ferretti, P. Di Rosa, J. Villaescusa- Ramirez, L. Fernandez, G. Ferrari, F. Blasi. University Vita Salute San Raffaele, Milano, Italy; IFOM (FIRC Institute of Molecular Oncology), Milano, Italy
493 B130 A novel zebrafish ets domain protein Etsrp is a master regulator of vasculogenesis. S. Sumanas, S. Lin. University of California, Los Angeles
494 B131 The role of Xenopus Disabled-2 in embryonic angiogenesis. S. Cheong, J. Han. Division of Molecular and Life Sciences, POSTECH, San 31, Hyoja-Dong, Pohang, Kyungbuk, 790-784, Republic of Korea
495 B132 Lymphangiogenesis in Xenopus laevis. R.E. Ka¨lin, M.P. Kretz, I. Senn, A.W. Bra¨ndli. Instit of Pharmaceutical Sci, Swiss Fed Instit of Tech, Zu¨rich, Switzerland CH-8093
496 B133 Gene profiling of early hematopoietic mesoderm in Xenopus. D.A. Yergeau, J.R. Doherty, E. Kuliyev, G.A. Neale, P.E. Mead. Department of Pathology; Hartwell Center for Bioinformatics and Biotechnology; St Jude Children’s Research Hospital, Memphis, TN 38105
497 B134 Low levels of BMP signaling promote gene expression in the intermediate mesoderm in a cell autonomous, but indirect manner. R.G. James, T.M. Schultheiss. Harvard University; Beth Israel Deaconess Medical Center, Boston, MA 02215
498 B135 Inversin is required for normal nodal flow in Kupffer’s vesicle and left-right development in zebrafish. P.A. Sacayon, J.J. Essner, H.J. Yost. Hunstman Cancer Institute, Center for Children, Department of Oncological Sciences, University of Utah
499 B136 Cited2 is required for heart morphogenesis and establishment of the left-right axis in mouse. S.L. Dunwoodie, K. Lopes Floro, W.J. Weninger, J.I. Preis, T.J. Mohun. Victor Chang Cardiac Research Institute, Sydney 2010, Australia; Medical University Vienna, A-1090 Austria; National Institute for Medical Research, Mill Hill NW71aa, UK
500 B137 A mutagenesis screen to identify genes involved in Drosophila early gonad formation. J.J. Weyers, A.B. Milutinovich, M. Van Doren. Johns Hopkins University, Dept. of Biology
501 B138 Does FOXL2 regulate aromatase expression during avian sex determination? Q.J. Hudson, C.A. Smith, A.H. Sinclair. Department of Paediatrics, University of Melbourne and Murdoch Childrens Research Institute, Royal Children’s Hospital, Melbourne, Australia
502 B139 NHERF2/SIP-1 interacts with mouse SRY via a different recognition mechanism than human SRY. L. Thevenet, K.H. Albrecht, S. Malki, P. Berta, B. Boizet-Bonhoure, F. Poulat. Institut de Ge´ne´tique Humaine, CNRS UPR 1142, Montpellier, France; Boston University, School of Medicine, Boston, MA 02118 USA; Universite´deNıˆmes, Nıˆmes, France
503 B140 Identification of genes involved in ovary development using microarrays. H. Lee, K. Albrecht. Boston University School of Medicine, Boston, MA 02118
504 B141 FKBP52 is required for steroidal regulation of external genital development and fertility. H. Chen, I. Wolf, Z. Yang, S. Periyasamy, E. Sanchez, W. Shou. Indiana University School of Medicine, Indianapolis, IN 46202; Medical College of Ohio, Toledo, OH 43614
505 B142 External genitalia formation; a model system to study organogenesis. G. Yamada. Center for Animal Resources and Development (CARD)
506 B143 Genome-wide transcriptional activity in wild-type and diseased mouse placentas. K.S. Knox, J.C. Baker. Department of Genetics, Stanford University, Stanford, CA
507 B144 Uncovering a role for Patched1 in epidermal homeostasis: interfollicular epidermal hyperplasia in Ptcmes/mes mutants. E. Nieuwenhuis, C. Hui. Program in Developmental Biology The Hospital for Sick Children, Department of Molecular and Medical Genetics University of Toronto, Canada
508 B145 Runx3 plays a role in hair structure determination. U. Gat, E. Raveh, S. Cohen, D. Levanon, Y. Groner. Department of Cell and animal Biology, Silberman Life Sciences Institute, The Hebrew University in Jerusalem, 91904, Israel; Department of Molecular Genetics, The Weizmann Institute of Science, Rehovot 76100, Israel
509 B146 Fetal breathing-like movements are essential for lung cell differentiation. M.R. Inanlou, B. Kablar. Dalhousie University, Halifax, NS, Canada B3H 4H7
510 B147 Nmyc plays an essential role during lung development as a dosage sensitive regulator of progenitor cell proliferation and differentiation. T. Okubo, P.S. Knoepfler, R.N. Eisenman, B.L. Hogan. Department of Cell Biology, Duke University Medical Center; Division of Basic Sciences, Fred Hutchinson Cancer Research Center 568 Developmental Biology Volume 283, 2005
511 B148 Mapping the gene expression program of the embryonic mouse lung. F. Espinoza, M.A. Krasnow. Stanford University School of Medicine and Howard Hughes Medical Institute
512 B149 Genomic analysis of the initiation of Drosophila respiratory system organogenesis by the Trachealess bHLH transcription factor. E.D. Chao, M.A. Krasnow. Department of Biochemistry and HHMI, Stanford University School of Medicine, Stanford, CA 94305-5307
513 B150 From signalling pathways to cellular reorganisation during tracheal morphogenesis in Drosophila. V. Brodu, J. Casanova. Institut de Biologia Molecular de Barcelona (IBMB), CSIC, Barcelona, Spain
514 B151 Patterns of airway branching during mouse lung development. R.J. Metzger, M.A. Krasnow. Howard Hughes Medical Institute and Stanford University School of Medicine
515 B152 Essential function of FGF signaling pathway during branching morphogenesis of mammary gland. P. Lu, A. Ewald, G. Martin, Z. Werb. Department of Anatomy, School of Medicine, University of California, San Francisco, CA 94143
516 B153 Spry4 is an antagonist of FGF8 and is required for proper AER development. B.D. Yu, G. Minowada, G.R. Martin. Department in Dermatology, School of Medicine, University of California, San Francisco; University Hospitals of Cleveland, Case Western Reserve University, Cleveland; Department of Anatomy and Program in Developmental Biology, School of Medicine, University of California- San Francisco, CA
517 B154 Tbx4 is dispensable for post-bud hindlimb outgrowth. L.A. Naiche, V.E. Papaioannou. Dept. of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
518 B155 Live three-dimensional OPT imaging and tomographic reconstruction of limb development. M.J. Boot, J. Sanz, M. Torres, J. Sharpe. MRC Human Genetics Unit, Edinburgh, United Kingdom; Centro Nacional de Biotecnologia, Madrid, Spain
519 B156 How thyroid hormone controls limb development during Xenopus laevis metamorphosis. L. Cai, B. Das, D.D. Brown. Department of Embryology, Carnegie Institution of Washington
520 B157 Dwarf zebrafish mutant for trpm7 exhibit defective skeletogenesis and kidney stone formation. M.R. Elizondo, E.L. MacDonald, B.L. Arduini, J. Paulsen, J.L. Sabel, P.D. Henion, R.A. Cornell, D.M. Parichy. Department of Biology, University of Washington; Center for Molecular Neurobiology, Ohio State University; Department of Anatomy and Cell Biology, University of Iowa
521 B158 The analysis of Myf5À/À:MyoDÀ/À amyogenic embryos reveals importance of early muscle contractions in mouse skeletogenesis. I. Rot- Nikcevic, T. Reddy, K.J. Downing, B. Kablar. Dalhousie University, Halifax, NS, Canada B3H 4H7
522 B159 The odd-skipped-related transcription factors regulate boundaries of craniofacial skeletal development in mice. Y. Lan, Q. Wang, K.M. Maltby, R. Jiang. Center for Oral Biology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642
523 B160 Withdrawn
524 B161 Wnt9a is required for maintenance of synovial joint integrity and temporal regulation of chondrocyte maturation. D. Spaeter, C. Hartmann. Institute of Molecular Pathology, Vienna 1030, Austria
525 B162 Canonical Wnt signaling is required for pronephric kidney tubule development. J.P. Lyons, T. Deroo, H. Ji, E.A. Jones, K. Vleminckx, P.D. McCrea. University of Texas M.D. Anderson Cancer Center, GSBS Program in Genes and Development, Houston, TX 77030; Ghent University, B-9052 Ghent, Belgium; University of Warwick, Coventry, UK
526 B163 Requirement of the mesonephros for the specification of the indifferent gonad. J.B. Scott, T.M. Schultheiss. Harvard Medical School; Beth Israel Deaconess Medical Center
527 B164 Growth and development of the mu¨llerian duct: cellular behavior and its relation to the wolffian duct. G.D. Orvis, R.R. Behringer. University of Texas M.D. Anderson Cancer Center, Houston
528 B165 Hypoplasia, duplex ureters and hydronephrosis in p53-deficient mice. Z.R. Saifudeen, J. Stefkova, S. Dipp, S.S. El-Dahr. Department of Pediatrics, Tulane University Health Sciences Center, New Orleans, LA 70112
529 B166 The mouse Fgf8 gene is required for nephrogenesis but not for somitogenesis. M. Lewandoski, O. Timofeeva, F. Naillat, C. Richman, S. Pajni-Underwood, C. Wilson, S. Vainio, A.O. Perantoni. Cancer and Developmental Biology Laboratory, and Laboratory of Comparative Carcinogenesis, National Cancer Institute, Frederick, MD 21702; Biocenter Oulu and Department of Biochemistry, Faculties of Science and Medicine, University of Oulu, Finland
530 B167 The role of Gata3 during kidney development. D. Grote, A. Souabni, M. Busslinger, M. Bouchard. McGill University, Montre´al, Canada; Research Institute of Molecular Pathology, Vienna, Austria
531 B168 Forming organs from tubes: identification of a common transcriptionally regulated morphogenetic step for many organs. T. Evans, A. Holtzinger. Albert Einstein College of Medicine, Bronx, NY 10461 SDB Meeting Abstracts 569
532 B169 Novel functions for GATA4 revealed by the zebrafish model. A. Holtzinger, T. Evans. Department of Developmental and Molecular Biology, Albert Einstein College of Medicine
533 B170 The role of BMP signaling in patterning the common thymus–parathyroid primordium. J. Gordon, S.R. Patel, F. Mahbub, N.R. Manley. University of Georgia, Athens, GA 30602
534 B171 Genetic analysis of the role of Gcm2 in parathyroid organogenesis and PTH expression. Z. Liu, N.R. Manley. Genetics, University of Georgia, Athens, GA 30602
535 B172 Jagged2-Notch1 signaling is required for proper oral epithelial differentiation and palate development. L.M. Casey, Y. Lan, E. Cho, K.M. Maltby, T. Gridley, R. Jiang. University of Rochester School of Med. and Dent., Rochester, NY 14642; Chonbuk Nat. University School of Dent., Chonju, Rep. of Korea; The Jackson Lab, Bar Harbor, ME 04609
536 B173 Mammalian Twisted Gastrulation gene loss of function and embryonic Salivary Gland Branching. T. Jaskoll, A. Petryk, G. Abichaker, D. Witcher, M. Melnick. University of Southern California, Los Angeles, CA 90089-0641; University of Minnesota, Minneapolis, MN 55455-0356
537 B174 The making of the liver. C.S. Lee, J.R. Friedman, J.T. Fulmer, K.H. Kaestner. University of Pennsylvania, Philadelphia, PA 19104
538 B175 Genetic and genomic approaches to study liver development in zebrafish. J. Peng, H. Huang, L. Guo, C. Gao, W. Cheng, M. Aw, J. Chen, E. Ng. Functional Genomics Laboratory, Institute of Molecular and Cell Biology, Singapore
539 B176 Vhnf1 integrates BMP, RA, and FGF signaling pathways to regulate pancreas development in zebrafish. J. Song, H. Kim, S. Lin. University of California, Los Angeles, CA 90095
540 B177 Cdx4 is required to correctly position the zebrafish pancreas. M.D. Kinkel, M. Alonzo, D. Stafford, V. Prince. The University of Chicago, Chicago, IL 60637
541 B178 Manic Fringe induces endocrine differentiation through transcription factor Myt1. G. Gu, Y. Xu, S. Wang, J. Zhang, A. Zhao. Vanderbilt Medical Center, Dept. of Cell and Dev. Biol., Nashville, TN 37232
542 B179 Role of Ptf1a in exocrine and endocrine pancreas. Z.H. Jarikji, M.E. Horb. University de Montreal, Canada; Institut de Recherches Cliniques de Montreal (IRCM), Canada
543 B180 The basic leucine zipper transcription factor MafB regulates glucagon gene transcription and is critical for alpha and beta cell development. I. Artner, J. Le Lay, B. Blanchi, M. Sieweke, R. Stein. Vanderbilt University Medical Center, Nashville, TN 37232; Centre d’Immunologie de Marseille-Luminy, Marseille, France
544 B181 Identification and characterization of CTGF as a factor involved in islet development and beta cell expansion. L.A. Wilding, E. Tweedie, B. Boone, D.R. Brigstock, K.M. Lyons, S. Levy, M.A. Gannon. Vanderbilt University, Nashville, TN 37232; The Ohio State University, Columbus, OH 43212; University of California, Los Angeles, Los Angeles, CA 90095
545 B182 Nkx2.2 functions as a transcriptional repressor in islet development. M.J. Doyle, K. Anderson, L. Sussel. University of Colorado HSC, Aurora, CO 80045
546 B183 Gene expression analysis of embryonic pancreas differentiation. K. Juhl, G. Gradwohl, J. Jensen, J. Hutton. BDC, UCHSC, Denver, CO 80262, USA; INSERM, Unit 381, 67200, Strasbourg, France
547 B184 Sympathetic innervation of the murine pancreas during development. R.E. Burris, M. Hebrok. UC San Francisco Diabetes Center, San Francisco, CA 94143-0540
548 B185 The regulation of sympathetic nervous system development by BMP and bHLH factors. Y. Morikawa, B. Genc, T.M. Washington, P. Cserjesi. Dept. Cell and Molecular Biology, Tulane University, New Orleans, LA 70118
549 B186 Migratory behaviors of adrenomedulla-fated neural crest cells are instructed by the adrenocortical cells. D. Saito, Y. Takahashi. Center for Developmental Biology, RIKEN, Kobe, Japan
550 B187 Chick–Quail intestinal transplants—A new method for studying enteric nervous system development. N. Nagy, G.M. Allan. Department of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
Morphogenesis
551 B188 The C. elegans VAB-1/Eph receptor tyrosine kinase functions with the SAX-3/Robo receptor during cell moments and axon guidance. I.D. Chin-Sang. Queen’s University, Kingston ON, Canada K7L 3N6
552 B189 A screen for epithelial defects in the Drosophila ovary reveals a role for cytoplasmic Dynein in the targeting of apical proteins. S. Horne-Badovinac, D. Bilder. Department of Molecular and Cell Biology, UC Berkeley 570 Developmental Biology Volume 283, 2005
553 B190 A two-dimensional mathematical model for dorsal appendage cell patterning in Drosophila. B.S. Gardiner, D.W. Smith, P. Pivonka. University of Melbourne, Parkville VIC 3010 Australia
554 B191 In toto imaging of organogenesis in zebrafish. S.G. Megason, S.E. Fraser. California Institute of Technology, Pasadena, CA 91125
555 B192 Differentiation of a sexually dimorphic spinal nucleus in a teleost fish. N.L. Rivera-Rivera, J.L. Serrano-Velez, E. Rosa-Molinar. University of Puerto Rico-Rio Piedras, PR 00931-1809
556 B193 Role of desmosomal cadherins in zebrafish vascular morphogenesis. M.J. Harrington, M. Tsang, I. Dawid, M. Halpern, R. Brewster. University of MD Baltimore County, MD 21250; University of Pittsburgh School of Medicine, PA 15261; NICHD, National Institutes of Health, MD; Carnegie Institution of Washington, MD 21210
557 B194 The role of N-cadherin in zebrafish neurulation. E. Hong, M. Tsang, M. Halpern, R. Brewster. University of Maryland Baltimore County, Baltimore, MD 21228; University of Pittsburgh School of Medicine, Pittsburgh, PA 15261; Carnegie Institution, Baltimore, MD 21210
558 B195 Role of non-classical cadherins in shaping the zebrafish neural tube. F.C. Fasanmi, G.M. Ayalew, R. Brewster. University of Maryland Baltimore County, Baltimore, MD 21250
559 B196 Differentially directed cell movements drive heart tube assembly in zebrafish. N.S. Glickman, H. Tsai, D. Yelon. Skirball Institute, NYU School of Medicine, New York, NY; National Taiwan University, Taipei, Taiwan
560 B197 trilobite/van gogh-like 2 regulates polarized cell behaviors underlying directed migration. J.R. Jessen, L. Solnica-Krezel. Vanderbilt University, Nashville, TN
561 B198 Investigating Naked function during Wnt and EGFR signaling. T.J. Van Raay, L. Solnica-Krezel, R.J. Coffey. Vanderbilt University, Nashville, TN
562 B199 A cell cycle regulatory gene, gadd45b is involved in zebrafish mesoderm patterning. K.S. Brown, S.L. Amacher. Department of Molecular and Cell Biology, University of California, Berkeley, CA
563 B200 Role of Hedgehog signaling in anterior neurocranium development. J.K. Eberhart, M.E. Swartz, C.B. Kimmel. University of Oregon, Eugene, OR 97403
564 B201 New functions for a vertebrate Rho guanine nucleotide exchange factor in cilia-dependent processes. J.R. Panizzi, J.R. Jessen, L. Solnica- Krezel. Vanderbilt University, Nashville, TN
565 B202 Coordinating the left-right (LR) asymmetry along zebrafish posterior-anterior axis by Southpaw (Spw) Wave. X. Wang, J.D. Amack, B.W. Bisgrove, J.J. Essner, J.H. Yost. University of Utah, Salt Lake City, UT 84108
566 B203 Analysis of the tight junction component claudin d during zebrafish development. A.E. Bruce, Z.S. Jeyapalan, M. Dixon Fox, I. Skromne. University of Toronto, Toronto, ON M5S3G5; University of Chicago, Chicago, IL 60637
567 B204 Integrin Alpha5 and Delta/Notch signalling have complementary spatiotemporal requirements during zebrafish somitogenesis. S.A. Holley, D. Ju¨lich, R. Geisler. Yale University, New Haven, CT 06520; Max Planck-Institut fu¨r Entwicklungsbiologie, Tu¨bingen, D-72076
568 B205 Interactions between muscle fibers and segment boundaries in zebrafish. C.A. Henry, W.A. Durst, S.E. Munchel, S.L. Amacher. University of Maine, Orono, ME; University of California, Berkeley, CA
569 B206 The effects of the widely used herbicide atrazine on early amphibian development. J.R. Lenkowski, K.A. McLaughlin. Department of Biology, Tufts University, Medford, MA 02155
570 B207 Cell behaviors associated with somite formation in Xenopus laevis. M. Ho, B. Glosier, C. Meloty, K. Wunderlich, J. Gustin, C. Domingo. Dept. of Biology, SFSU, San Francisco, CA 94132; Dept. of Cell Biology, UC Davis, Davis, CA; Biomolecular Resource Center, UCSF, San Francisco, CA 94143; Oregon Health Sciences University, Portland, OR 97201
571 B208 Syndecan-4 regulates convergent extension movements in Xenopus embryos. J. Larrain, M. Moreno, C. Oliva, R. Mun˜oz. P. Universidad Catolica de Chile, Fondap-Biomedicine
572 B209 The KIAA0888 homolog of Xenopus tropicalis is essential for early embryogenesis. M.E. Torrejon, R. Gupta, K. Walter, S. Gennuso, S. Reinsch. NASA-Ames Research Center, Moffett Field, CA
573 B210 Analysis of Xenopus laevis claudins (Xcla) tight junction genes in development. J. Xie, B. Brizuela. North Carolina State University, Raleigh, NC 27695
574 B211 A nodal ligand and its inhibitor define a left-right organizer in Xenopus neurula. A. Vonica, A.H. Brivanlou. Rockefeller University, New York, NY 10021 SDB Meeting Abstracts 571
575 B212 Shroom is a multifunctional protein that initiates a suite of different cell behaviors during neural tube closure. C. Lee, J.B. Wallingford. University of Texas, Austin, TX 78712
576 B213 XNF-ATc3 is necessary for morphogenesis of the Xenopus neural tube. A.G. Borchers, J.C. Baker. Department of Genetics, Stanford University Medical School, Stanford, CA 94062, USA
577 B214 time-lapse analysis of epidermal cell behavior during vertebrate neurulation. J.M. Hayes, E.K. Kieserman, C. Lee, J.B. Wallingford. University of Texas at Austin
578 B215 The role of calcium in somite morphogenesis. A. Harstock, C. Meloty-Kapella, M.T. Ho, C.R. Domingo. Dept. of Biology, SFSU, San Francisco, CA 94132; Dept. of Cell Biology, UCD, Davis CA
579 B216 Vertebrate orthologues of the Drosophila PCP genes Fuzzy and Inturned play unexpected roles craniofacial morphogenesis. J.B. Wallingford, T. Park, S.L. Haigo, S.M. Peyrot. University of Texas, Austin; University of California, Berkeley
580 B217 Correct subcellular localization of Dishevelled is essential for planar cell polarity and Rho/Rac activation but not for canonical Wnt signaling in vertebrate embryos. T. Park, R.S. Gray, R. Habas, J.B. Wallingford. University of Texas-Austin; Robert Wood Johnson School of Medicine
581 B218 Tumorhead function in Xenopus and fission yeast possibly involves PAK signaling. C. Wu, P. Yang, E.E. Traverso, N. Morin, S. Marcus, L.D. Etkin. University of Texas M.D. Anderson Cancer Center, Houston, TX 77030; Centre de Recherche de Biochimie macromole´culaire, CNRS, Montpellier, France
582 B219 New tools for quantitative analysis of morphogenesis in spherical embryos. J.M. Tyszka, A.E. Ewald, J.B. Wallingford, S.E. Fraser. Biological Imaging Center, Division of Biology, California Institute of Technology, Pasadena, California, USA; Department of Anatomy, University of California, San Francisco, California, USA; Institute for Cellular and Molecular Biology, University of Texas
583 B220 Tension affects apoptosis in chick chorioamniotic membrane. R.E. Pulver, B. Holton. University of Wisconsin Oshkosh
584 B221 Indian hedgehog regulated skeletal angiogenesis in association with cartilage and bone development. C. Colnot, L. de la Fuente, S. Huang, D. Hu, C. Lu, B. St- Jacques, J.A. Helms. Departments of Orthopaedic Surgery, University of California, San Francisco, California, 94143- 0514, USA; Department of Plastic and Reconstructive Surgery, Stanford University, Stanford CA. 94305, USA; Genetics Unit, Shriners Hospital, Montreal, Quebec, Canada
585 B222 The cytoplasmic C-terminal tail of Claudin-1 is essential for randomizing the direction of heart looping in chick embryos. E. Di Pietro, A. Simard, A.K. Ryan. Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada
586 B223 SDF1 and its receptor CXCR4 control cell migration and patterning during somitogenesis. E. Ohata, T. Kasai, T. Nagasawa, Y. Takahashi. NAIST, Nara, Japan; Center for Developmental Biology, RIKEN, Kobe, Japan; Kyoto University, Kyoto, Japan
587 B224 Activation of Notch in the posterior compartment of a somite determines the differentiation, directed migration, and patterning of the dorsal aorta precursors. Y. Sato, T. Watanabe, J. Kouyama, H. Okano, Y. Takahashi. Center for Developmental Biology, RIKEN, Kobe, Japan; NAIST, Nara, Japan; Keio University School of Medicine, Tokyo, Japan
588 B225 Novel localization of Claudin-16 tight junction protein in the goblet cells of chick intestine. O. Ozden, B. Black, B. Brizuela. North Carolina State University, Raleigh, NC 27695
589 B226 Optic cup formation requires downregulation of Sox3 expression in chick embryonic eye. Y. Ishii, T. Mikawa. Weill Med. Col. Cornell University, New York, NY10021
590 B227 TIMP-2 regulates cardiac neural crest morphogenesis in avian embryos. M.V.Reedy, V.Cantemir, M. Holmberg, J. Sanmann, M. Stroscheim, D. Cai, P.R. Brauer. Department of Biology, Creighton University, Omaha NE; Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE
591 B228 Control of dorsal-ventral innervation choice in the vertebrate limb. V. Luria, E. Laufer. Columbia University, New York, NY 10032
592 B229 The adapter protein SH2-B regulates nerve growth factor-induced gene expression. L. Chen, C. Carter-Su. University of Michigan Medical School
593 B230 Identification and developmental expression analysis of a novel homeobox gene located in the proximal region of mouse Chromosome 18. H. Liu, W. Liu, K.M. Maltby, Y. Lan, R. Jiang. University of Rochester, Rochester, NY 14627; University of Rochester School of Medicine and Dentistry, Rochester, NY 14642
594 B231 Wnt5a regulates SHH and FGF10 signaling during lung development. C. Li, L. Hu, J. Xiao, H. Chen, J. Li, S. Bellusci, S. Delanghe, P. Minoo. University of Southern California, Los Angeles, CA 90033; Dalian Medical University, Dalian 116027, China; Saban Research Institute of Childrens Hospital Los Angeles, Los Angeles, CA 90027 572 Developmental Biology Volume 283, 2005
595 B232 The transcription factor HNF-6 controls differentiation of ductal pancreatic cells. C.E. Pierreux, S. Cordi, F. Clotman, M. Maestro, J. Ferrer, G.G. Rousseau, F.P. Lemaigre. Universite´ catholique de Louvain and Institute of Cellular Pathology, Brussels, Belgium; Institut d’Investigacions Biome`diques August Pi i Sunyer, Barcelona, Spain
596 B233 Identification of a novel gene expressed exclusively in the zone of polarizing activity in the vertebrate limb. J.R. Rock, B.D. Harfe. University of Florida, Gainesville, FL 32610
597 B234 MicroRNA regulation of murine limb development. D.M. Maatouk, M.T. McManus, B.D. Harfe. University of Florida, Gainesville, FL 32610; University of California, San Francisco, San Francisco, CA 94143
598 B235 Molecular cascade leading to the morphological segmentation during somitogenesis: Ephrin-Eph signaling downstream of Notch and cMeso1. T. Watanabe, Y. Sato, J. Kouyama, H. Okano, Y. Takahashi. NAIST, Nara, Japan; Center for Developmental Biology RIKEN, Kobe, Japan; Keio University School of Medicine, Tokyo, Japan
599 B236 Failure of TGF-h signaling via Alk5 in neural crest cells leads to facial clefting. M. Dudas, J. Kim, A. Nagy, J. Larsson, S. Karlsson, V. Kaartinen. Developmental Biology Program, Childrens Hospital Los Angeles, Los Angeles, CA 90027, USA; Lund University Hospital, Sweden
600 B237 Craniofacial dysmorhogenesis in qkk2 Quaking null mutation embryos. O.v. Lakiza, J. Northrop, M. Justice, D. Walterhouse, E.B. Goodwin, P. Iannaccone. Children’s Memorial Research Center, Northwestern University Feinberg School of Medicine, Chicago, IL; University of Wisconsin, Madison, WI; Baylor College of Medicine, Houston, TX
601 B238 Fgfr1 and Fgfr2 are necessary for craniofacial development in the mouse. G. Smyth, K. Yu, D.M. Ornitz, J. Partanen, E.N. Meyers. Duke University Medical Center, Durham, NC 27710; Washington University Medical School, St. Louis, MO 63110; Viikki Biocenter, Developmental Biology Program, PO Box 56, Viikinkaari 9, 00014-University of Helsinki, Finland
602 B239 Regulation of craniofacial morphogenesis by Dlx family members. P.G. Farlie, S.J. McKeown, D.F. Newgreen. Murdoch Childrens Research Institute, Melbourne, Victoria 3052, Australia
603 B240 Perioptic mesenchymal target for retinoic acid synthesized in eye field ectoderm. A. Molotkov, N. Molotkova, G. Duester. The Burnham Institute, La Jolla, CA 92037
604 B241 A novel gene required during neural tube and eye development. T. Kim, K.V. Anderson, L. Niswander. Cornell University and Sloan- Kettering Institute, New York, NY; University of Colorado Health Science Center, Denver, CO
605 B242 The actin binding protein Shroom facilitates neural tube closure by regulating the apical positioning of actomyosin. J.D. Hildebrand. Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260
606 B243 Spontaneous mutation in mice causes branching of the notochord and fusions of caudal vertebrae. D.L. Chapman, D.R. Farkas. Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260
607 B244 Nap1-dependent cell migration is required for early organogenesis and axis specification in the mouse. A.S. Rakeman, K.V. Anderson. WGSMS, Cornell University, 445 East 69th Street New York, NY 10021; Sloan Kettering Institute, 1275 York Ave. New York, NY 10021
608 B245 The chato mutation disrupts antero-posterior axis elongation of the mouse embryo. M.J. Garcia-Garcia, K.V. Anderson. Sloan-Kettering Institute. New York, NY 10021
609 B246 limulus and wsnp: cell adhesion during embryonic morphogenesis. J.D. Lee, K.V. Anderson. Sloan Kettering Institute, New York, NY 10021
610 B247 Middle primitive streak assembly and function during mouse gastrulation. E. Lacy, S. Strope, R. Rivi, C. Munoz, F. Lupu, L. Jerome- Majewska. Sloan-Kettering Institute, NY
611 B248 A polarity gene, Pals1 (Stardust), is essential for normal gastrulation in mice. S. Kim, N. Dunn, J. Malicki, C.A. Walsh. HHMI/BIDMC, Harvard Med. School, Boston, MA 02115; Harvard University Cambridge, MA 02138 and ES Cell International, Singapore 138667; Harvard Med. School/ MEEI, Boston, MA 02114
Cell Motility and Guidance
612 B249 GON-1 and fibrulin have antagonistic roles in control of organ shape. D. Hesselson, C. Newman, K. Kim, J. Kimble. Dept. Genetics; Dept. Biochemistry; HHMI and Depts Biochemistry and Genetics, University of Wisconsin-Madison, Madison, WI 53706
613 B250 Planarian Robo RNAi knockdowns result in CNS patterning defects and disruption of polarity during regeneration. F. Cebria`, P.A. Newmark. Department of Cell and Structural Biology, Neuroscience Program, University of Illinois at Urbana-Champaign, IL 61801, USA
614 B251 Cell motility and steroid hormone signaling in Drosophila. M.A. Starz-Gaiano, X. Wang, S. Bridges, M. Melani, D. Montell. Johns Hopkins School of Medicine, Baltimore, MD SDB Meeting Abstracts 573
615 B252 Dissecting the role of VEGFR in hemocyte migration in Drosophila. M. Haesemeyer, D. Siekhaus, R. Lehmann. HHMI, Developmental Genetics Program, NYU School of Medicine, New York, NY 10016
616 B253 At the next stop sign, turn right: The metalloprotease tolloid-related1 controls the defasciculation of motoaxons in Drosophila. H. Aberle, F. Meyer. MPI for Developmental Biology, Spemannstr.35, 72076 Tuebingen, Germany
617 B254 The role of Eph/ephrin signaling during neuronal migration. T.M. Coate, P.F. Copenhaver. Oregon Health and Sciences University, Portland, OR 97239
618 B255 Slit gradient in the brain: neurobiologists’ imagination? M. Furrer, I. Vasenkova, D. Kamiyama, O. Unluer, Y. Rosado, A. Chiba. Cell and Dev. Biol., University of Illinois, Urbana, IL
619 B256 Role of slits in longitudinal axon guidance. H.F. Nural, A.L. Altick, W.T. Farmer, G.S. Mastick. University of Nevada, Reno, NV 89557
620 B257 Sonic Hedgehog sets dorsal-ventral positioning of early longitudinal axon tracts. W.T. Farmer, A.L. Altick, T. Hailstone, G.S. Mastick. University of Nevada, Reno
621 B258 C-ephexin regulates motor axon stalling at the limb base. C.E. Krull, M. Sahin, M.Z. Lin, J. Eberhart, M.E. Greenberg. University of Michigan, Ann Arbor, MI 48109; Harvard University, Boston, MA 02115; University of Missouri-Columbia, Columbia, MO 65202
622 B259 Evidence that motor growth cones use physical force to exit from the spinal cord. K.W. Tosney, D. Dehnbostel, A. Wagnitz, K. Balazovich. The University of Michigan, Ann Arbor, MI
623 B260 Rac1 regulates integrin-dependent adhesion in neuronal growth cones. S. Woo, T.M. Gomez. University of Wisconsin-Madison
624 B261 Molecular control of axon arborization of trigeminal sensory neurons. M. Choy, A. Sagasti, A.F. Schier. Skirball Institute, New York, NY 10016
625 B262 Nevermind/CYFIP2 is required for dorsoventral optic tract sorting and topographic mapping in the zebrafish retinotectal system. A.J. Pittman, C. Chien. University of Utah Medical Center, Salt Lake City, UT 84132
626 B263 Expression of metalloproteinase ADAM10 in the developing visual system of Xenopus laevis. Y. Chen, J.C. Hocking, C.L. Hehr, S. McFarlane. Genes and Development Research Group, University of Calgary, Health Sciences Centre, Calgary, Alberta T2N 4N1, Canada
627 B264 Beta-catenin independently specifies pathfinding and targeting of optic axons in the tectum. K. Mirro, M. Lee, H. Muhr, T. Elul. Touro University College of Osteopathic Medicine, Vallejo, CA 94592
628 B265 A diffusible guidance cue, Wnt3, is a candidate opposing signal for medial-lateral retinotectal mapping. Y. Zou, A. Schmitt, J. Shi, A. Wolf, C. Lu, L. King. Department of Neurobiology, Pharmacology and Physiology, Committees on Developmental Biology and Neurobiology, the University of Chicago
629 B266 Inhibition of semaphorin signaling by Dlx homeobox genes promotes tangential migration of interneurons from the basal forebrain to the neocortex. S. Cheng, T. Le, J. Wigle, D. Eisenstat. Depts. of Pediatrics, Biochemistry and Anatomy, University of Manitoba, Winnipeg, Canada
630 B267 Pax6À/À mice have defects in non-radial cell migration and interneuron specification. P.P. Gopal, J.A. Golden. Department of Neuroscience, University of Pennsylvania; Department of Pathology, Children’s Hospital of Philadelphia, PA 19104
631 B268 The a5 subunit of Laminin is required for proper murine neural crest cell migration. E.G. Coles, L.S. Gammill, J.H. Miner, M. Bronner- Fraser. Division of Biology, California Institute of Technology, Pasadena, CA 91125; Washington University School of Medicine, St. Louis, MO 63110
632 B269 Identification of genes involved in the induction and migration of neural crest cells. M.S. Adams, L.S. Gammill, M. Bronner-Fraser. Division of Biology, California Institute of Technology, Pasadena, CA 91125
633 B270 Chain migration of cranial neural crest cells, a dynamic in vivo analysis. P.A. Rupp, P.M. Kulesa. Stowers Institute for Medical Research, Kansas City, MO
634 B271 Cranial neural crest migration in the avian embryo and the role of Ephrin-A5. C.C. Lu, S.E. Fraser. California Institute of Technology, Pasadena, CA 91125
635 B272 Segmental migration of neural crest cells through the somites requires neuropilin-2/semaphorin3F signaling. L.S. Gammill, C. Gonzalez, C. Gu, D. Ginty, M. Bronner-Fraser. Division of Biology 139-74, California Institute of Technology, Pasadena, CA 91125; Department of Neuroscience and Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, Baltimore, MD 21205 574 Developmental Biology Volume 283, 2005
Molecular Medicine and Development
636 B273 Fetal Alcohol Syndrome: The effects of ethanol on murine forelimb and hindlimb development. Y.G. Yueh, C. Cheng, J. Janitell, T. Thal, D.P. Gardner. Midwestern University, Glendale, AZ 85308
637 B274 OFD1, a human disease gene, is required for the assembly of cilia and centrosomes. V.I. Singla, A.R. Norman, J.F. Reiter. University of California, San Francisco
638 B275 Dosage-sensitive interaction of TBX1 and CRKL underlies the aetiology of del22q11 syndrome. D.L. Guris, V.E. Papaioannou, A. Imamoto. Committee on Developmental Biology, The Ben May Institute for Cancer Research, University of Chicago, Chicago, IL 60637; Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032
639 B276 Protease-activated receptors (PARs) in mouse embryonic development. A.M. Cheng, S.R. Coughlin. University of California, San Francisco, CA 94143
640 B277 Hoxb13 and Nkx3.1 have overlapping and distinct functions in the mouse prostate. K.D. Economides, M.R. Capecchi, C. Abate-Shen. CABM-UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854; University of Utah, Salt Lake City, UT 84112
641 B278 Corneal disease in mice expressing a dominant-negative Clim/Ldb transcriptional cofactor. X. Xu, J. Spencer, B. Andersen. Departments of Biological Chemistry and Medicine, University of California, Irvine, CA 92697
642 B279 Cation channel TRPM7 is necessary for survival of embryonic melanophores in zebrafish and is expressed in human melanoma cell lines. R.A. Cornell, J. Paulsen, M. Elizondo, B. Arduini, E. Burninght, P.D. Henion, D. Parichy, M. Hsu. University of Iowa, Iowa City, IA 52242; University of Texas, Austin, TX 7812; Ohio State University, Columbus, OH 43210
ACKNOWLEDGMENTS
Artwork for the SDB 64th Annual Meeting poster and the Program/Abstracts booklet cover was provided by Michelle Starz-Gaiano, Johns Hopkins School of Medicine, Baltimore, MD. Grants and Awards: National Science Foundation (NSF), National Institute of Child and Human Health and Development (NICHD), National Institute of Dental and Craniofacial Research (NIDCR), National Institute of Neurological Diseases and Stroke (NINDS), March of Dimes Birth Defects Foundation, Federation of American Societies for Experimental Biology/Minority Access to Research Careers Program (FASEB/MARC). Corporate Sponsors: Developmental Biology, Developmental Cell, Elsevier, eInstruction Corporation, Gene Tools, LLC, Intavis AG, Carl Zeiss MicroImaging, Inc. Exhibitors: Aquatic Habitats; Cold Spring Harbor Lab Press; The Company of Biologists Ltd; eInstruction Corporation; Elsevier; Hoelle and Huettner AG; Intavis AG; Leica Microsystems; Morton Publishing Company; Nature Publishing Group; Olympus America; St. Jude Children’s Research Hospital; John Wiley and Sons; Carl Zeiss MicroImaging, Inc.