RESEARCH ARTICLE Low cost, low tech SNP genotyping tools for resource-limited areas: Plague in Madagascar as a model Cedar L. Mitchell1¤a, Voahangy Andrianaivoarimanana2, Rebecca E. Colman1¤b, Joseph Busch1, Heidie Hornstra-O'Neill1, Paul S. Keim1,3, David M. Wagner1, Minoarisoa Rajerison2*, Dawn N. Birdsell1* 1 The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America, 2 Plague Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar, 3 Translational a1111111111 Genomics Research Institute, Flagstaff, Arizona, United States of America a1111111111 a1111111111 ¤a Current address: Department of Epidemiology, Gillings School of Global Public Health, University of North a1111111111 Carolina, Chapel Hill, North Carolina, United States of America a1111111111 ¤b Current address: Department of Medicine, University of California, San Diego, La Jolla, California, United States of America *
[email protected] (MR);
[email protected] (DNB) OPEN ACCESS Abstract Citation: Mitchell CL, Andrianaivoarimanana V, Colman RE, Busch J, Hornstra-O'Neill H, Keim PS, Background et al. (2017) Low cost, low tech SNP genotyping Genetic analysis of pathogenic organisms is a useful tool for linking human cases together tools for resource-limited areas: Plague in Madagascar as a model. PLoS Negl Trop Dis 11 and/or to potential environmental sources. The resulting data can also provide information (12): e0006077. https://doi.org/10.1371/journal. on evolutionary patterns within a targeted species and phenotypic traits. However, the pntd.0006077 instruments often used to generate genotyping data, such as single nucleotide polymor- Editor: Cheryl Andam, University of New phisms (SNPs), can be expensive and sometimes require advanced technologies to imple- Hampshire, UNITED STATES ment.