Current Developments and Challenges in Plant Viral Diagnostics: a Systematic Review
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Genetic Diversity of Dengue Virus in Clinical Specimens from Bangkok
Tropical Medicine and Infectious Disease Article Genetic Diversity of Dengue Virus in Clinical Specimens from Bangkok, Thailand, during 2018–2020: Co-Circulation of All Four Serotypes with Multiple Genotypes and/or Clades Kanaporn Poltep 1,2,3 , Juthamas Phadungsombat 2,4 , Emi E. Nakayama 2,4 , Nathamon Kosoltanapiwat 1 , Borimas Hanboonkunupakarn 5, Witthawat Wiriyarat 3, Tatsuo Shioda 2,4,* and Pornsawan Leaungwutiwong 1,* 1 Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand; [email protected] (K.P.); [email protected] (N.K.) 2 Mahidol-Osaka Center for Infectious Diseases (MOCID), Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand; [email protected] (J.P.); [email protected] (E.E.N.) 3 The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand; [email protected] 4 Department of Viral Infections, Research Institute for Microbial Diseases (RIMD), Osaka University, Osaka 565-0871, Japan 5 Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand; [email protected] * Correspondence: [email protected] (T.S.); [email protected] (P.L.) Citation: Poltep, K.; Phadungsombat, Abstract: Dengue is an arboviral disease highly endemic in Bangkok, Thailand. To characterize J.; Nakayama, E.E.; Kosoltanapiwat, the current genetic diversity of dengue virus (DENV), we recruited patients with suspected DENV N.; Hanboonkunupakarn, B.; infection at the Hospital for Tropical Diseases, Bangkok, during 2018–2020. We determined complete Wiriyarat, W.; Shioda, T.; nucleotide sequences of the DENV envelope region for 111 of 276 participant serum samples. -
Grapevine Virus Diseases: Economic Impact and Current Advances in Viral Prospection and Management1
1/22 ISSN 0100-2945 http://dx.doi.org/10.1590/0100-29452017411 GRAPEVINE VIRUS DISEASES: ECONOMIC IMPACT AND CURRENT ADVANCES IN VIRAL PROSPECTION AND MANAGEMENT1 MARCOS FERNANDO BASSO2, THOR VINÍCIUS MArtins FAJARDO3, PASQUALE SALDARELLI4 ABSTRACT-Grapevine (Vitis spp.) is a major vegetative propagated fruit crop with high socioeconomic importance worldwide. It is susceptible to several graft-transmitted agents that cause several diseases and substantial crop losses, reducing fruit quality and plant vigor, and shorten the longevity of vines. The vegetative propagation and frequent exchanges of propagative material among countries contribute to spread these pathogens, favoring the emergence of complex diseases. Its perennial life cycle further accelerates the mixing and introduction of several viral agents into a single plant. Currently, approximately 65 viruses belonging to different families have been reported infecting grapevines, but not all cause economically relevant diseases. The grapevine leafroll, rugose wood complex, leaf degeneration and fleck diseases are the four main disorders having worldwide economic importance. In addition, new viral species and strains have been identified and associated with economically important constraints to grape production. In Brazilian vineyards, eighteen viruses, three viroids and two virus-like diseases had already their occurrence reported and were molecularly characterized. Here, we review the current knowledge of these viruses, report advances in their diagnosis and prospection of new species, and give indications about the management of the associated grapevine diseases. Index terms: Vegetative propagation, plant viruses, crop losses, berry quality, next-generation sequencing. VIROSES EM VIDEIRAS: IMPACTO ECONÔMICO E RECENTES AVANÇOS NA PROSPECÇÃO DE VÍRUS E MANEJO DAS DOENÇAS DE ORIGEM VIRAL RESUMO-A videira (Vitis spp.) é propagada vegetativamente e considerada uma das principais culturas frutíferas por sua importância socioeconômica mundial. -
Vector Capability of Xiphinema Americanum Sensu Lato in California 1
Journal of Nematology 21(4):517-523. 1989. © The Society of Nematologists 1989. Vector Capability of Xiphinema americanum sensu lato in California 1 JOHN A. GRIESBACH 2 AND ARMAND R. MAGGENTI s Abstract: Seven field populations of Xiphineraa americanum sensu lato from California's major agronomic areas were tested for their ability to transmit two nepoviruses, including the prune brownline, peach yellow bud, and grapevine yellow vein strains of" tomato ringspot virus and the bud blight strain of tobacco ringspot virus. Two field populations transmitted all isolates, one population transmitted all tomato ringspot virus isolates but failed to transmit bud blight strain of tobacco ringspot virus, and the remaining four populations failed to transmit any virus. Only one population, which transmitted all isolates, bad been associated with field spread of a nepovirus. As two California populations of Xiphinema americanum sensu lato were shown to have the ability to vector two different nepoviruses, a nematode taxonomy based on a parsimony of virus-vector re- lationship is not practical for these populations. Because two California populations ofX. americanum were able to vector tobacco ringspot virus, commonly vectored by X. americanum in the eastern United States, these western populations cannot be differentiated from eastern populations by vector capability tests using tobacco ringspot virus. Key words: dagger nematode, tobacco ringspot virus, tomato ringspot virus, nepovirus, Xiphinema americanum, Xiphinema californicum. Populations of Xiphinema americanum brownline (PBL), prunus stem pitting (PSP) Cobb, 1913 shown through rigorous test- and cherry leaf mottle (CLM) (8). Both PBL ing (23) to be nepovirus vectors include X. and PSP were transmitted with a high de- americanum sensu lato (s.1.) for tobacco gree of efficiency, whereas CLM was trans- ringspot virus (TobRSV) (5), tomato ring- mitted rarely. -
Interaction of Tobacco Etch Virus and the Root-Knot Nematode, Meloidogyne Incognita in Chile Pepper, Capsicum Frutescens
Interaction of tobacco etch virus and the root-knot nematode, Meloidogyne incognita in chile pepper, Capsicum frutescens Item Type text; Thesis-Reproduction (electronic) Authors Koenning, Stephen Robert Publisher The University of Arizona. Rights Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. Download date 25/09/2021 20:46:59 Link to Item http://hdl.handle.net/10150/555147 INTERACTION OF TOBACCO ETCH VIRUS AND THE ROOT-KNOT NEMATODE, MELOIDOGYNE INCOGNITA IN CHILE PEPPER, CAPSICUM FRUTESCENS by Stephen Robert Koenning A Thesis Submitted to the Faculty of the DEPARTMENT OF PLANT PATHOLOGY In Partial Fulfillment of the Requirements For the Degree of MASTER OF SCIENCE In the Graduate College THE UNIVERSITY OF ARIZONA 1 9 7 9 STATEMENT BY AUTHOR This thesis has been submitted in partial fulfill ment of requirements for an advanced degree at The University of Arizona and is deposited in the University Library to be made available to borrowers under rules of the Library. Brief quotations from this thesis are allowable without special permission, provided that accurate acknowl edgment of source is made. Requests for permission for extended quotation from or reproduction of this manuscript in whole or in part may be granted by the head of the major department or the Dean of the Graduate College when in his judgment the proposed use of the material is in the inter ests of scholarship. -
Transmission of Virus by the Progeny of Crosses Between Xiphinema Diversicaudatum
Transmission of virus by the progeny of crosses between XQhinema diversicaudatüm (Nematoda : Dorylaimoidea) from Italy and Scotland Derek J. F. BROWN Scottish Crop Research Institute, Invergowrie, Dundee, 002 SDA, Scotland. SUMMARY Transmission of the type-British strainsof arabis mosaic (AMV-T) and strawberry latent ringspot viruses (SLRV-T) and a strain of SLRV from Italy (SLRV-Ip) by FI and F2 hybrid Xiphinenza diversicaudatum was examined in the laboratory. The hybrid nematodes were crossbred from populations which readily (Scotland) and only infrequently (Italy) transmitted viruses.The ability of X. diversicaudatum hybrids to transmit viruses was foundto be inherited withthe choice of both maternaland paternal parents affecting the hybrids ability to transmit viruses. It is possible that the genetic influence on the hybrids ability to transmit viruses was cytoplasmically inherited. The principal factor likely to be involved is the ability of X. di ver sic au da tu??^ selectively and specifically to retain virus particles at sites of retention within its feeding apparatus. RBSUME La transnzission des virus par la descendance de croiselnents entre Xiphjnema diversicaudatum (Nernatoda :Do ylainzoidea) provenantd’ltalie et d’Ecosse La transmission de souches de type britannique des virus de la mosaïque arabis (AMV-T), du virus du G ringspot n latent du fraisier (SLRV-T)et d’une souchede SLRV provenant d‘Italie (SLRV-Ip) par des hybridesFI et F2 de Xiphinewa diversicaudatuwz .a été Ctudiée au laboratoire. Les nématodes hybrides étaient obtenus par croisements entre populations qui transmettent les virus soit activement (Ecosse), soit seulement occasionnellement (Italie). La capacité de transmission des virus montrée par les X. -
Effectiveness of Seed Treatments in Reducing TMV Infection Tanner A
Effectiveness of Seed Treatments in Reducing TMV Infection Tanner A. Robison Mentor: Claudia Nischwitz Department of Biology, Utah State University, Logan, Utah, USA Abstract Germination Rate Tobacco mosaic virus (TMV), named for the plant it was first discovered in, 1 can infect hundreds of different species. It has no known invertebrate 0.9 vectors, but TMV is spread mechanically through contact with other plants, 0.8 clothes, tools, contaminated soil and seedborne. While TMV is known to be 0.7 exceptionally stable, it has been reported that treatment of tools with 0.6 powdered milk can significantly reduce the infection rate of the virus. While 0.5 there is a seed treatment available for conventional agriculture, there is no 0.4 seed treatment available for organic production. Organic growers, who grow 0.3 heirloom tomatoes experience the highest rates of TMV infection. Since 0.2 powdered milk has been reported as an effective means of treatment in 0.1 tools, the purpose of this research is to determine whether it can be an 0 Almond Soy Rinsed Unrinsed Control effective seed treatment. To test this, tomato seeds were treated with powdered milk, soy milk, almond milk and water. A grow out test was then Results/Discussion conducted in a greenhouse and plants were tested for infection using In the grow out trial little effect was seen. The infection rate for Polymerase Chain Reaction and antibody based ELISA testing. In the first grow powdered milk, almond milk, soy milk and control treatments out trial treatment little effect was seen, with infection rate for the powdered were 61%, 34%, 32% and 47%, respectively. -
Induction of Plant Resistance Against Tobacco Mosaic Virus Using the Biocontrol Agent Streptomyces Cellulosae Isolate Actino 48
agronomy Article Induction of Plant Resistance against Tobacco Mosaic Virus Using the Biocontrol Agent Streptomyces cellulosae Isolate Actino 48 Gaber Attia Abo-Zaid 1 , Saleh Mohamed Matar 1,2 and Ahmed Abdelkhalek 3,* 1 Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria 21934, Egypt; [email protected] (G.A.A.-Z.); [email protected] (S.M.M.) 2 Chemical Engineering Department, Faculty of Engineering, Jazan University, Jazan 45142, Saudi Arabia 3 Plant Protection and Biomolecular Diagnosis Department, ALCRI, City of Scientific Research and Technological Applications, New Borg El Arab city, Alexandria 21934, Egypt * Correspondence: [email protected] Received: 8 September 2020; Accepted: 19 October 2020; Published: 22 October 2020 Abstract: Viral plant diseases represent a serious problem in agricultural production, causing large shortages in the production of food crops. Eco-friendly approaches are used in controlling viral plant infections, such as biocontrol agents. In the current study, Streptomyces cellulosae isolate Actino 48 is tested as a biocontrol agent for the management of tobacco mosaic virus (TMV) and inducing tomato plant systemic resistance under greenhouse conditions. Foliar application of a cell pellet suspension of Actino 48 (2 107 cfu. mL 1) is performed at 48 h before inoculation with TMV. Peroxidase activity, × − chitinase activity, protein content, and the total phenolic compounds are measured in tomato leaves at 21 dpi. On the other hand, the TMV accumulation level and the transcriptional changes of five tomato defense-related genes (PAL, PR-1, CHS, PR-3, and PR-2) are studied. -
Journal of Virological Methods 153 (2008) 16–21
Journal of Virological Methods 153 (2008) 16–21 Contents lists available at ScienceDirect Journal of Virological Methods journal homepage: www.elsevier.com/locate/jviromet Use of primers with 5 non-complementary sequences in RT-PCR for the detection of nepovirus subgroups A and B Ting Wei, Gerard Clover ∗ Plant Health and Environment Laboratory, Investigation and Diagnostic Centre, MAF Biosecurity New Zealand, P.O. Box 2095, Auckland 1140, New Zealand abstract Article history: Two generic PCR protocols were developed to detect nepoviruses in subgroups A and B using degenerate Received 21 April 2008 primers designed to amplify part of the RNA-dependent RNA polymerase (RdRp) gene. It was observed that Received in revised form 17 June 2008 detection sensitivity and specificity could be improved by adding a 12-bp non-complementary sequence Accepted 19 June 2008 to the 5 termini of the forward, but not the reverse, primers. The optimized PCR protocols amplified a specific product (∼340 bp and ∼250 bp with subgroups A and B, respectively) from all 17 isolates of the 5 Keywords: virus species in subgroup A and 3 species in subgroup B tested. The primers detect conserved protein motifs Nepoviruses in the RdRp gene and it is anticipated that they have the potential to detect unreported or uncharacterised Primer flap Universal primers nepoviruses in subgroups A and B. RT-PCR © 2008 Elsevier B.V. All rights reserved. 1. Introduction together with nematode transmission make these viruses partic- ularly hard to eradicate or control (Harrison and Murant, 1977; The genus Nepovirus is classified in the family Comoviridae, Fauquet et al., 2005). -
Cucumber Mosaic Kymberly R
XHT1255 Provided to you by: Cucumber Mosaic Kymberly R. Draeger*, UW-Madison Plant Pathology What is cucumber mosaic? Cucumber mosaic is a viral disease of worldwide distribution that affects over 1200 plant species. Hosts include a wide range of fruits, vegetables, herbaceous and woody ornamentals, and weeds. The disease has perhaps its biggest impact in vegetable production where it can cause significant losses in yield and vegetable quality. arden Facts Cumber mosaic on pepper (left) showing yellowing and ring spots, and on broad bean (right) showing mosaic and puckering of leaf tissue. (Photos courtesy of Russ Groves) What does cucumber mosaic look like? Symptoms of cucumber mosaic can vary widely depending on host species, host variety, and time of infection. Typical symptoms include stunting of entire plants, mosaic or mottling (i.e., blotchy white, yellow, and light green areas) and ring spots (i.e., ring-like areas of discolored tissue) on leaves and fruits, and a variety of growth distortions such as cupping, puckering and strapping (i.e., elongation and thinning) of leaves as well as warts on fruits. In extreme situations, parts of an affected plant or even an entire plant may die from the disease. Where does cucumber mosaic come from? Cucumber mosaic is caused by Cucumber mosaic virus (CMV) which can overwinter in susceptible biennial or perennial weeds, as well as in perennial agricultural crops (e.g., alfalfa) and perennial herbaceous and woody ornamentals. Seeds and even pollen from certain host plants can carry the virus, and thus the virus can be spread via these plant parts. -
Hantavirus Disease Were HPS Is More Common in Late Spring and Early Summer in Seropositive in One Study in the U.K
Hantavirus Importance Hantaviruses are a large group of viruses that circulate asymptomatically in Disease rodents, insectivores and bats, but sometimes cause illnesses in humans. Some of these agents can occur in laboratory rodents or pet rats. Clinical cases in humans vary in Hantavirus Fever, severity: some hantaviruses tend to cause mild disease, typically with complete recovery; others frequently cause serious illnesses with case fatality rates of 30% or Hemorrhagic Fever with Renal higher. Hantavirus infections in people are fairly common in parts of Asia, Europe and Syndrome (HFRS), Nephropathia South America, but they seem to be less frequent in North America. Hantaviruses may Epidemica (NE), Hantavirus occasionally infect animals other than their usual hosts; however, there is currently no Pulmonary Syndrome (HPS), evidence that they cause any illnesses in these animals, with the possible exception of Hantavirus Cardiopulmonary nonhuman primates. Syndrome, Hemorrhagic Nephrosonephritis, Epidemic Etiology Hemorrhagic Fever, Korean Hantaviruses are members of the genus Orthohantavirus in the family Hantaviridae Hemorrhagic Fever and order Bunyavirales. As of 2017, 41 species of hantaviruses had officially accepted names, but there is ongoing debate about which viruses should be considered discrete species, and additional viruses have been discovered but not yet classified. Different Last Updated: September 2018 viruses tend to be associated with the two major clinical syndromes in humans, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary (or cardiopulmonary) syndrome (HPS). However, this distinction is not absolute: viruses that are usually associated with HFRS have been infrequently linked to HPS and vice versa. A mild form of HFRS in Europe is commonly called nephropathia epidemica. -
Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies
viruses Review Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies Varvara I. Maliogka 1,* ID , Angelantonio Minafra 2 ID , Pasquale Saldarelli 2, Ana B. Ruiz-García 3, Miroslav Glasa 4 ID , Nikolaos Katis 1 and Antonio Olmos 3 ID 1 Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece; [email protected] 2 Istituto per la Protezione Sostenibile delle Piante, Consiglio Nazionale delle Ricerche, Via G. Amendola 122/D, 70126 Bari, Italy; [email protected] (A.M.); [email protected] (P.S.) 3 Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia, Spain; [email protected] (A.B.R.-G.); [email protected] (A.O.) 4 Institute of Virology, Biomedical Research Centre, Slovak Academy of Sciences, Dúbravská cesta 9, 84505 Bratislava, Slovak Republic; [email protected] * Correspondence: [email protected]; Tel.: +30-2310-998716 Received: 23 July 2018; Accepted: 13 August 2018; Published: 17 August 2018 Abstract: Perennial crops, such as fruit trees, are infected by many viruses, which are transmitted through vegetative propagation and grafting of infected plant material. Some of these pathogens cause severe crop losses and often reduce the productive life of the orchards. Detection and characterization of these agents in fruit trees is challenging, however, during the last years, the wide application of high-throughput sequencing (HTS) technologies has significantly facilitated this task. In this review, we present recent advances in the discovery, detection, and characterization of fruit tree viruses and virus-like agents accomplished by HTS approaches. -
OCCURRENCE of STONE FRUIT VIRUSES in PLUM ORCHARDS in LATVIA Alina Gospodaryk*,**, Inga Moroèko-Bièevska*, Neda Pûpola*, and Anna Kâle*
PROCEEDINGS OF THE LATVIAN ACADEMY OF SCIENCES. Section B, Vol. 67 (2013), No. 2 (683), pp. 116–123. DOI: 10.2478/prolas-2013-0018 OCCURRENCE OF STONE FRUIT VIRUSES IN PLUM ORCHARDS IN LATVIA Alina Gospodaryk*,**, Inga Moroèko-Bièevska*, Neda Pûpola*, and Anna Kâle* * Latvia State Institute of Fruit-Growing, Graudu iela 1, Dobele LV-3701, LATVIA [email protected] ** Educational and Scientific Centre „Institute of Biology”, Taras Shevchenko National University of Kyiv, 64 Volodymyrska Str., Kiev 01033, UKRAINE Communicated by Edîte Kaufmane To evaluate the occurrence of nine viruses infecting Prunus a large-scale survey and sampling in Latvian plum orchards was carried out. Occurrence of Apple mosaic virus (ApMV), Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV), Apple chlorotic leaf spot virus (ACLSV), and Plum pox virus (PPV) was investigated by RT-PCR and DAS ELISA detection methods. The de- tection rates of both methods were compared. Screening of occurrence of Strawberry latent ringspot virus (SLRSV), Arabis mosaic virus (ArMV), Tomato ringspot virus (ToRSV) and Petunia asteroid mosaic virus (PeAMV) was performed by DAS-ELISA. In total, 38% of the tested trees by RT-PCR were infected at least with one of the analysed viruses. Among those 30.7% were in- fected with PNRSV and 16.4% with PDV, while ApMV, ACLSV and PPV were detected in few samples. The most widespread mixed infection was the combination of PDV+PNRSV. Observed symptoms characteristic for PPV were confirmed with RT-PCR and D strain was detected. Com- parative analyses showed that detection rates by RT-PCR and DAS ELISA in plums depended on the particular virus tested.