Etude Chimique Et Statistique De La Composition D'huiles Essentielles D

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Etude Chimique Et Statistique De La Composition D'huiles Essentielles D Etude chimique et statistique de la composition d’huiles essentielles d’origans (Lamiaceae) cultivés issus de graines d’origine méditerranéenne Gilles Figueredo To cite this version: Gilles Figueredo. Etude chimique et statistique de la composition d’huiles essentielles d’origans (Lami- aceae) cultivés issus de graines d’origine méditerranéenne. Chimie organique. Université Blaise Pascal - Clermont-Ferrand II, 2007. Français. NNT : 2007CLF21732. tel-00717749 HAL Id: tel-00717749 https://tel.archives-ouvertes.fr/tel-00717749 Submitted on 13 Jul 2012 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Numéro d’ordre : D.U. 1732 UNIVERSITÉ BLAISE PASCAL (U.F.R. Sciences et technologie) ÉCOLE DOCTORALE DES SCIENCES FONDAMENTALES N° 525 THÈSE présentée pour obtenir le grade de DOCTEUR D’UNIVERSITÉ (Spécialité : Chimie organique) Par Gilles FIGUEREDO Ingénieur CNAM option Chimie Organique Étude chimique et statistique de la composition d’huiles essentielles d'origans (Lamiaceae) cultivés issus de graines d'origine méditerranéenne Soutenue publiquement le 20 février 2007, devant la commission d’examen Président : M. Yves TROIN, Professeur à l’E.N.S.C. de Clermont -Ferrand Rapporteurs : Mme Chantal MENUT, Professeur à l’E.N.S.C. de Montpellier M. André-Paul CARNAT, Maître de Conférences à l’Université d’Auvergne Examinateurs M Jean-Claude CHALCHAT, Maître de Conférences à l’Université Blaise Pascal M. Raymond-Philippe GARRY, Professeur à l’I.U.F.M. de Clermont-Ferrand M. Bernard PASQUIER, Directeur du C.N.P.M.A.I. Milly-la-Forêt Invité M. Jean-Marie BESSIÈRE, Professeur à l’E.N.S.C. de Montpellier à Martine REMERCIEMENTS Ce travail a été effectué sous la direction de Monsieur Jean-Claude CHALCHAT au sein de l’équipe de recherche du Laboratoire de Chimie des Hétérocycles et des Glucides dirigé par Monsieur Yves TROIN, Professeur à l'E.N.S.C. de Clermont-Ferrand. J’exprime mes remerciements et une vive reconnaissance à Monsieur Jean-Claude CHALCHAT qui a initié ce travail et qui m'a conseillé, encouragé et soutenu scientifiquement tout au long de ces quatre années. Á Monsieur Yves TROIN qui m'a accueilli au sein de son laboratoire et pour avoir accepté la présidence du jury de thèse, j'exprime ma plus grande gratitude Mes remerciements s’associent de la même manière à Monsieur André Paul CARNAT, Maître de Conférences à l’Université d’Auvergne ainsi que Madame Chantal MENUT, Professeur à l'E.N.S.C de Montpellier qui m’ont fait l’honneur de juger mon travail et d’en être les rapporteurs. Je remercie vivement Monsieur Bernard PASQUIER, Directeur du C.N.P.M.A.I. à Milly-la- Forêt pour nous avoir fourni l’ensemble des huiles essentielles et les données botaniques du Genre Origanum. Je tiens également à remercier Monsieur Raymond-Philippe GARRY, Professeur à l’I.U.F.M. de Clermont-Ferrand et M. Jean-Marie BESSIERE Professeur à l'E.N.S.C de Montpellier pour avoir accepté d’examiner ce travail. J'exprime mes plus vifs et sincères remerciements à Madame Andrée CARNAT qui a su me faire partager toutes ses connaissances, mais aussi pour sa patience et le temps qu'elle a su consacrer à la finalité de mon travail. Je remercie Monsieur SCHOEFFERT du C.F.P.P.A. de Nyons pour nous avoir procuré les échantillons de O. grosii. Enfin je tiens à remercier le personnel du Service d’Imprimerie de l’Université Blaise Pascal qui a contribué à l’impression et à la mise en forme finale. SOMMAIRE SOMMAIRE ........................................................................................................ 1 INTRODUCTION............................................................................................... 4 CHAPITRE 1 : Généralités sur les matières odorantes .................................. 7 1.1. - Historique ................................................................................................................................................................. 7 1.2. - Différents procédés d’obtention de matières parfumées........................................................................................... 9 1.2.1. - La digestion............................................................................................................................................... 9 1.2.2. - L’enfleurage............................................................................................................................................ 10 1.2.3. - Extraction par solvants volatils ............................................................................................................... 10 1.2.3.1. - L’alcool éthylique....................................................................................................................... 10 1.2.3.2. - L’éther éthylique......................................................................................................................... 11 1.2.3.3. - D’autres solvants......................................................................................................................... 12 1.3. - Généralités sur les épices........................................................................................................................................ 12 1.4. - Huiles essentielles .................................................................................................................................................. 14 1.4.1. - Définition ................................................................................................................................................ 14 1.4.1.1. - Concrète...................................................................................................................................... 16 1.4.1.2. - Pommade florale......................................................................................................................... 16 1.4.1.3. - Résinoïde .................................................................................................................................... 16 1.4.1.4. - Absolue....................................................................................................................................... 17 1.4.2. - Répartition, localisation .......................................................................................................................... 17 1.4.3. - Rôle des huiles essentielles dans la plante .............................................................................................. 18 1.4.4. - Obtention des huiles essentielles............................................................................................................. 19 1.4.4.1. - Par expression des épicarpes de Citrus ....................................................................................... 19 1.4.4.2. - Par distillation en présence d’eau............................................................................................... 20 1.4.4.3. – Par extraction aux gaz supercritiques......................................................................................... 20 1.4.5. - Facteurs de variabilité ............................................................................................................................. 22 1.4.5.1. - Diversité selon l’organe végétal.................................................................................................. 22 1.4.5.2. - Influence de la période de récolte, du climat et du sol................................................................ 22 1.4.5.3. - Influence des différents paramètres sur la qualité des huiles essentielles ................................... 22 1.4.5.4. - Existence de variétés chimiques ou chémotypes......................................................................... 25 1.4.6. - Utilisation des huiles essentielles en aromathérapie................................................................................ 25 1.4.7. - Méthodes d’identification des composés ................................................................................................ 26 1.4.7.1. - La chromatographie en phase gazeuse........................................................................................ 26 1.4.7.2. - Indice de rétention ...................................................................................................................... 27 1.4.8. - Dégradation............................................................................................................................................. 29 CHAPITRE 2 : Biosynthèse des constituants volatils et odorants................ 30 2.1. - Les terpènes et terpénoïdes..................................................................................................................................... 30 2.1.1. - Formation du motif isoprénique.............................................................................................................. 31 2.1.2. - Condensation des motifs isopréniques .................................................................................................... 32 2.1.3. - Transformations secondaires..................................................................................................................
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