Side 124-235 26/03/04 15:03 Side 187 POSTER VIIIp-1 CLONING AND HETEROLOGOUS EXPRESSION OF A GENE ENCODING PYRANOSE OXIDASE FROM THE WHITE-ROT FUNGUS TRAMETES MULTICOLOR 1 1,2 3 3 1 1 Christian LEITNER , HOANG Lan , Petr Halada , Jindrich VOLC , Klaus D. KULBE , Dietmar HALTRICH and Clemens K. 1 PETERBAUER 1 Division of Food Biotechnology, Dept. of Food Sciences & Technology, University of Natural Resources and Applied Life Sciences (BOKU) Muthgasse 18, A-1190 Wien, Austria; tel +43-1-360066274, fax +43-1-360066251,
[email protected] 2 Institute of Biological and Food Technology, Hanoi University of Technology, Dai Co Viet Road, Hanoi, Vietnam 3 Institute of Microbiology, Academy of Sciences of the Czech Republic Vídenská 1083, CZ-142 20 Praha 4, Czech Republic Pyranose oxidase (pyranose 2-oxidase, P2O; EC 1.1.3.10) is an enzyme that widely occurs in basidiomycetous fungi. It catalyzes the C-2 oxidation of several aldopyranoses to form the respective 2-keto derivatives, transferring electrons to molecular oxygen to yield hydrogen peroxide. There is indication of an involvement of P2O in lignocellulose degradation; the exact physiological role of P2O, however, is not clearly understood to date. P2O is a potentially interesting enzyme for biotechnological applications, its reaction products (2-keto sugars) can be attractive inter- mediates in the production of food additives, such as fructose, tagatose, or isomaltulose, and can easily be produced in high yields. Trametes multicolor is a potent producer of P2O activity and forms P2O constitutively during growth on a number of carbon sources.