Anti-AID Antibody [EK2-5G9] (ARG23560)

ARG23560 Package: 25 μg anti-AID antibody [EK2-5G9] Store at: -20°C

Summary

Product Description Rat Monoclonal antibody [EK2-5G9] recognizes AID. Rat anti Human AID antibody, clone EK2-5G9 recognizes human Single-stranded DNA cytosine deaminase, also known as Activation-induced cytidine deaminase or Cytidine aminohydrolase. AID is a 198 amino acid ~24 kDa RNA-editing enzyme essential for the initiation of immunoglobulin class switch recombination, somatic hypermutation and gene conversion.AID is selectively expressed by germinal centre B cells and B cell-derived lymphomas (Basso et al. 2012). Mutation in the AICDA gene is responsible for the autosomal recessive condition Immunodeficiency with hyper-IgM 2 (HIGM2) A rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE resulting in extreme susceptibility to bacterial infection.

Tested Reactivity Hu

Tested Application ELISA, FACS, IHC-P, WB

Host Rat

Clonality Monoclonal

Clone EK2-5G9

Isotype IgG2b

Target Name AID

Antigen Species Human

Immunogen Synthetic peptide around aa. 185-198 of Human AID.

Conjugation Un-conjugated

Alternate Names Cytidine aminohydrolase; CDA2; HIGM2; Activation-induced cytidine deaminase; AID; EC 3.5.4.38; ARP2; Single-stranded DNA cytosine deaminase; HEL-S-284

Application Instructions

Application table Application Dilution

ELISA Assay-dependent

FACS Assay-dependent

IHC-P Assay-dependent

WB Assay-dependent

Application Note IHC-P: Antigen Retrieval: Boil tissue section in EDTA (pH 8.0). FACS: Membrane permeabilisation is required for this application. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Calculated Mw 24 kDa

Properties www.arigobio.com 1/2 Form Liquid

Purification Purification with Protein G.

Buffer PBS and 0.09% Sodium azide.

Preservative 0.09% Sodium azide

Concentration 1 mg/ml

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol AICDA

Gene Full Name activation-induced cytidine deaminase

Background This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq, Feb 2009]

Function Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. [UniProt]