DOCSLIB.ORG

ISOLASI SENYAWA TRITERPENOID DARI EKSTRAK ASETON DAUN Garcinia Celebica L DAN UJI AKTIVITAS ANTIKANKER PAYUDARA (MCF-7)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
ISOLASI SENYAWA TRITERPENOID DARI EKSTRAK ASETON DAUN Garcinia Celebica L DAN UJI AKTIVITAS ANTIKANKER PAYUDARA (MCF-7) ISOLASI SENYAWA TRITERPENOID DARI EKSTRAK ASETON DAUN Garcinia celebica L DAN UJI AKTIVITAS ANTIKANKER PAYUDARA (MCF-7) SKRIPSI AMBAR ILAFAH RAMADHAN PROGRAM STUDI KIMIA FAKULTAS SAINS DAN TEKNOLOGI UNIVERSITAS ISLAM NEGERI SYARIF HIDAYATULLAH JAKARTA 2018 M / 1440 H ISOLASI SENYAWA TRITERPENOID DARI EKSTRAK ASETON DAUN Garcinia celebica L DAN UJI AKTIVITAS ANTIKANKER PAYUDARA (MCF-7) SKRIPSI Sebagai Salah Satu Syarat Memperoleh Gelar Sarjana Sains Program Studi Kimia Fakultas Sains Dan Teknologi Universitas Islam Negeri Syarif Hidayatullah Jakarta Oleh: AMBAR ILAFAH RAMADHAN 11140960000063 PROGRAM STUDI KIMIA FAKULTAS SAINS DAN TEKNOLOGI UNIVERSITAS ISLAM NEGERI SYARIF HIDAYATULLAH JAKARTA 2018 M / 1440 H ABSTRAK AMBAR ILAFAH RAMADHAN. Isolasi Senyawa Triterpenoid dari Ekstrak Aseton Daun Garcinia celebica L dan Uji Aktivitas Antikanker Payudara (MCF-7). Dibimbing oleh SRI HARTATI dan SITI NURBAYTI. Tumbuhan Garcinia celebica merupakan salah satu dari sekitar 450 spesies Garcinia yang mengandung senyawa triterpenoid, depsidon, xanton, dan benzofenon yang berpotensi sebagai terapi kanker. Uji pendahuluan antikanker payudara (MCF-7) terhadap ekstrak aseton daun G .celebica telah dilakukan dengan nilai aktivitas sebesar 94,36% dalam konsentrasi 200 µg/mL dan 83,12% dalam konsentrasi 50 µg/mL. Tujuan penelitian ini adalah mengisolasi dan mengidentifikasi struktur metabolit sekunder dari ekstrak aseton daun G. celebica serta aktivitas antikankernya. Tahapan yang dilakukan adalah fraksinasi menggunakan metode kromatografi, identifikasi struktur dengan spektroskopi UV- Vis, FTIR, LCMS, dan NMR serta uji aktivitas antikanker payudara (MCF-7) dengan metode MTT assays. GC-2 yang diperoleh berupa gum putih sebanyak 20 mg dari 47,7 g ekstrak kasar. Hasil analisis UV-Vis menunjukkan adanya gugus kromofor C=C (λmax 222 nm) dan C=O (λmax 272 nm). Analisis FTIR menunjukkan vibrasi dari gugus fungsi O-H karboksilat (3378,47 cm-1), C-O (1262,46 cm-1), C=O (1686,82 cm-1) dan C=C (1640,33 cm-1). Analisis LCMS menghasilkan puncak + + dominan m/z [M H] 453,4 (BM=452) dengan rumus molekul C30H44O3 diduga merupakan senyawa asam (24E)-3-okso-17,14-friedolanosta-8(9),14(15),24(25)- trien-26-oat. Analisis 1H NMR dan 13C NMR menunjukkan sinyal khas triterpenoid yaitu C=O keton (δc 211,2 C-3), -C=O karbonil karboksilat (δc 173,2 C-26), tiga C=C (δc 145,7 (C-14), 145,5 (C-25), 144,2 (C-9), 126,5 (C-25), 125,0 (C-8) dan 120,3 (C-15), 7 sinyal metil, 2 metin, 9 metilen dan 4 karbon kuartener. Hasil uji antikanker menunjukkan GC-2 memiliki aktivitas antikanker payudara yang sangat kuat dengan nilai IC50 sebesar 24,97 µg/mL. Kata kunci : Aktivitas antikanker, Garcinia celebica, isolasi, karakterisasi ABSTRACT AMBAR ILAFAH RAMADHAN. Isolation of Triterpenoid Compounds from Garcinia celebica L Leaf Acetone Extract and Breast Anticancer (MCF-7) Activity Test. Advisor by SRI HARTATI and SITI SURBAYTI. The Garcinia celebica plant is one of about 450 Garcinia species containing triterpenoid compounds, depsidone, xanthone and benzophenone which have the potential as cancer therapy. Preliminary test of breast anticancer (MCF-7) on the acetone extract of G .celebica leaf was carried out with an activity value of 94.36% in a concentration of 200 µg / mL and 83.12% in a concentration of 50 µg / mL. The purpose of this study was to isolate and identify secondary metabolite structure of G. celebica acetone extract and its anticancer activity. The steps taken were fractionation using chromatography method, structural identification with UV-Vis spectroscopy, FTIR, LCMS, and NMR as well as breast anticancer (MCF-7) activity test using MTT assays method. GC-2 obtained in the form of 20 mg of white gum from 47.7 g of crude extract. UV-Vis analysis shows that there is a chromophore C=C (λmax 222 nm) and C=O (λmax 272 nm). FTIR analysis showed the vibration of OH carboxylic functional groups (3.378,47 cm-1), C-O (1.262,46 cm-1), C=O (1.686,82 cm-1), and C=C (1.640,33 cm-1). The molecular formula C30H44O3 thought to be an acid compound (24E)-3-oxo-17,14-friedolanosta- 8(9),14(15),24(25)-trien-26-oat. The 1H NMR and 13C NMR analysis showed a typical triterpenoid signal, namely C=O ketone (δc 211,2 C-3), -C=O carboxylic carbonyl (δc 173,2 C-26), three C = C (δc 145,7 (C-14), 145,5 (C-25), 144,2 (C-9), 126,5 (C- 25), 125 (C-8) and 120,3 (C-15), 7 methyl signals, 2 metin, 9 methylene and 4 quaternary carbon.The results of anticancer tests showed GC-2 had very strong breast anticancer activity with IC50 value is 24,97 µg / mL. Keywords: Anticancer activity, Garcinia celebica, isolation, characterization KATA PENGANTAR Puji dan syukur penulis panjatkan kepada Allah Yang Maha Esa, karena berkat rahmat dan hidayah-Nya penulis dapat menyelesaikan skripsi yang berjudul “Isolasi Senyawa Triterpenoid dari Ekstrak Aseton Daun Garcinia celebica L dan Uji Aktivitas Antikanker Payudara (MCF-7)” Penulis menyadari bahwa terselesaikannya skripsi ini tak lepas dari bantuan dan peranan banyak pihak. Pada kesempatan ini, penulis mengucapkan terima kasih kepada: 1. Dr. Sri Hartati, M.Si selaku Pembimbing I yang telah memberikan pengarahan serta bimbingannya baik dalam teknis di lapangan maupun dalam menyelesaikan skripsi ini. 2. Dr. Siti Nurbayti, M.Si selaku Pembimbing II dan Pembimbing Akademik yang telah memberikan pengarahan serta bimbingannya sehingga banyak membantu penulis dalam menyelesaikan skripsi ini. 3. Tarso Rudiana, M.Si sebagai Penguji I yang telah memberikan saran serta masukan yang bermanfaat. 4. Nurhasni, M.Si sebagai Penguji II yang telah memberikan saran serta masukan yang bermanfaat. 5. Drs. Dede Sukandar, M.Si selaku Ketua Program Studi Kimia Fakultas Sains dan Teknologi UIN Syarif Hidayatullah Jakarta. 6. Dr. Agus Salim, M.Si selaku Dekan Fakultas Sains dan Teknologi UIN Syarif Hidayatullah Jakarta. 7. Isalmi Aziz, M.T selaku Sekretaris Program Studi Kimia Fakultas Sains dan Teknologi UIN Syarif Hidayatullah Jakarta. v 8. Bapak, Ibu, dan Adik tercinta atas segala doa, pengorbanan, nasihat dan motivasinya kepada penulis. 9. Segenap dosen Program Studi Kimia atas ilmu pengetahuan dan pegalaman hidup yang dengan ikhlas diajarkan dan diberikan kepada penulis. 10. Sahabat tersayang Esti, Ayu, Kak Yeni, Lucyta, Isni, Chinta, Nur Fauziyah, Nurlathifah, Afriana, Nur Azizah, Nur Ana, Indah, dan Nadhia yang senantiasa memberi bantuan, dukungan, nasihat dan motivasinya kepada penulis. 11. Teman–teman Kimia Angkatan 2014 yang senantiasa memberi dukungan, motivasi, dan keceriaan kepada penulis. 12. Serta semua pihak yang telah membantu secara langsung dan tidak langsung, yang tidak dapat disebutkan satu persatu. Penulis berharap skripsi ini dapat bermanfaat bagi penulis dan umumnya bagi kemajuan ilmu dan teknologi. Jakarta, Oktober 2018 Ambar Ilafah Ramadhan vi DAFTAR ISI Halaman KATA PENGANTAR ............................................................................................ v DAFTAR ISI ........................................................................................................ vii DAFTAR GAMBAR .............................................................................................. x DAFTAR TABEL ............................................................................................... xii DAFTAR LAMPIRAN ...................................................................................... xiii BAB I PENDAHULUAN ....................................................................................... 1 1.1 Latar Belakang ............................................................................................... 1 1.2 Rumusan Masalah .......................................................................................... 5 1.3 Hipotesis ........................................................................................................ 5 1.4 Tujuan Penelitian ........................................................................................... 6 1.5 Manfaat Penelitian ......................................................................................... 6 BAB II TINJAUAN PUSTAKA ........................................................................... 7 2.1 Tinjauan Umum Tumbuhan Garcinia celebica L ......................................... 7 2.1.1 Fitokimia Tumbuhan Garcinia ......................................................... 8 2.1.3 Aktivitas Biologis Metabolit Sekunder dari Garcinia .................... 18 2.2 Metode Isolasi Senyawa Aktif ..................................................................... 20 2.2.1 Ekstraksi .......................................................................................... 20 2.2.2 Kromatografi Lapis Tipis ................................................................ 21 2.3.3 Kromatografi Kolom ....................................................................... 22 2.3 Karakterisasi Struktur dengan Metode Spektroskopi .................................. 23 2.4.1 Spektroskopi UV-Vis ...................................................................... 24 2.4.2 Spektroskopi FTIR .......................................................................... 25 2.4.3 Spektroskopi Massa (MS) ............................................................... 26 2.4.4 Spektroskopi 1H NMR dan 13C NMR ............................................. 27 vii BAB III METODE PENELITIAN ..................................................................... 29 3.1 Tempat dan Waktu Penelitian ...................................................................... 29 3.2 Alat dan Bahan ...........................................................................................
Load more

Recommended publications
  • Downloaded From: Usage Rights: Creative Commons: Attribution-Noncommercial-No Deriva- Tive Works 4.0
    Trethowan, Liam Andrew (2018) Phytogeography and the stoichiometric niche: exploring their interplay in Sulawesi. Doctoral thesis (PhD), Manch- ester Metropolitan University in collaboration with Royal Botanic Gardens Kew. Downloaded from: https://e-space.mmu.ac.uk/623370/ Usage rights: Creative Commons: Attribution-Noncommercial-No Deriva- tive Works 4.0 Please cite the published version https://e-space.mmu.ac.uk PHYTOGEOGRAPHY AND THE STOICHIOMETRIC NICHE: EXPLORING THEIR INTERPLAY IN SULAWESI LIAM ANDREW TRETHOWAN A thesis submitted in partiAl fulfilment of the requirements of MAnchester MetropolitAn University for the degree of Doctor of Philosophy School of Science and Environment, Manchester MetropolitAn University in collaboration with RoyAl Botanic GArdens Kew 2018 1 For the people of SulAwesi whose goodwill, cheeriness and resourcefulness are the foundation of this thesis. They deserve fAr more than trAgedy. 2 Acknowledgements I thank the Indonesian Ministry for Research and Technology (RISTEK) for permission to perform fieldwork. Also, Herbarium Bogoriense (BO) for the Memorandum of Understanding and Material Transfer Agreement that supported my application to perform Indonesian fieldwork and to Deden Girmansyah and Endang Kintamani my counterparts from BO who provided support and key logistical help throughout my trip. Thanks to the Indonesian Agricultural Research Agency (Badan Litbang Kementerian Pertanian) for performing soil analyses. Funding for labwork and fieldwork was provided by the Bentham Moxon Trust, Coalbourn Trust, Botanical Research Fund, an MMU postgraduate research award and two Emily Holmes awards. I would like to thank numerous people from Sulawesi for their help and support; in particular researchers and Forestry Department workers: Ramadhanil Pitopang, Asrianti Arif, Rosmarlinasiah, Niken Purwijaya, Bambang, Saroso and Marcy Summers.
    [Show full text]
  • A Review on a Few Medicinal Plants Possessing Anticancer Activity Against Human Breast Cancer
    International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: 0974-4304 Vol.9, No.3, pp 333-365, 2016 A Review on a few medicinal plants possessing anticancer activity against human breast cancer Jaikumar B, Jasmine R* PG Research Department of Biotechnology, Bishop Heber College, Trichy-17, India Abstract: Breast Cancer is known to be the second most common cause of death. So there has been intense research on various plant resources to develop novel anticancer agents against breast cancer.Herbal medicine is one of the most commonly used complementary and alternative therapies (CAM) by people with cancer. Some studies have shown that as many as 6 out of every 10 people with cancer (60%) use herbal remedies alongside conventional cancer treatments. There are many different types of herbal medicines and some of them overlap with foods. Commonly used plants include Echinacea, St John’s Wort, green tea and ginger. From the past several years, medicinal plants have been proved to be an important natural source for cancer therapy with fewer side effects. There are many natural cytotoxic drugs available, which needs further improvement and development of new drugs. An attempt has been made to review some medicinal plants used for the prevention and treatment of cancer. This article considers a few medicinal plants used anticancer activity against breast cancer cell line(MCF- 7). It will be helpful to explore the medicinal value of plants and for new drug discovery from them for the researchers and scientists around the globe. Keywords: Medicinal plants, Anticancer, MTT assay, MCF-7 cells. Introduction Cancer is a general term applied of series of malignant diseases that may affect different parts of body.
    [Show full text]
  • Diversity of Garcinia Species in the Western Ghats: Phytochemical Perspective
    Diversity of Garcinia species in the Western Ghats: Phytochemical Perspective Editor K. B. Rameshkumar Jawaharlal Nehru Tropical Botanic Garden and Research Institute Thiruvananthapuram Title: Diversity of Garcinia species in the Western Ghats: Phytochemical Perspective Editor: K. B. Rameshkumar Published by: Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram 695 562, Kerala, India ISBN No.: 978-81-924674-5-0 Printed at: Akshara Offset, Thiruvananthapuram- 695 035 Copyright © 2016: Editor and Publisher All rights reserved. This book may not be reproduced in whole or in part without the prior written permission of the copyright owner. ii Foreword I am delighted to write a Foreword to the Book ‘Diversity of Garcinia species in the Western Ghats: Phytochemical Perspective’ edited by my student Dr. K. B. Rameshkumar who took Garcinia imberti as a subject for his doctoral studies. It gives me all the more pleasure and gratification to see that he continued with his studies on Garcinia species of the Western Ghats along with his students and colleagues. Unlike many other doctoral students, he kept alive his passion for the studies on Garcinia and the present book is the outcome of his dedicated efforts during the last one and a half decades. Pursuit of science is a passion and unravelling the subtleties of nature is an ecstasy which fulfils the inner urge for quest and discovery. The genus Garcinia is important by virtue of their reputation in traditional medicines, established pharmacological activities, diversity in chemical structures and potential nutritional properties. Despite recent progress in phytochemical and pharmacological studies on Garcinia species world over, significant gaps still exist concerning the exploration of the vast data on phytochemical diversity of Garcinia species.
    [Show full text]
  • 1–5 Rediscovery in Singapore of Calamus Densiflorus Becc
    NATURE IN SINGAPORE 2017 10: 1–5 Date of Publication: 25 January 2017 © National University of Singapore Rediscovery in Singapore of Calamus densiflorus Becc. (Arecaceae) Adrian H. B. Loo*, Hock Keong Lua and Wee Foong Ang National Parks Board HQ, National Parks Board, Singapore Botanic Gardens, 1 Cluny Road, Singapore 259569, Republic of Singapore; Email: [email protected] (*corresponding author) Abstract. Calamus densiflorus is a new record for Singapore after its rediscovery in the Rifle Range Road area in 2016. Its description, distribution and distinct vegetative characters are provided. Key words. Calamus densiflorus, new record, Singapore INTRODUCTION Calamus densiflorus Becc. is a clustering rattan palm of lowland forest and was Presumed Nationally Extinct in Singapore (Tan et al., 2008; Chong et al., 2009). This paper reports its rediscovery in the Rifle Range Road area in 2016 and reassigns it status in Singapore to “Critically Endangered” according to the categories defined in The Singapore Red Data Book (Davison et al., 2008). Description. Calamus densiflorus is a dioecious clustering rattan palm, climbing to 40 m tall (Fig. 1, p. 2). It has stems enclosed in bright yellowish green sheaths up to 4 cm wide. The spines are hairy, dense and slightly reflexed (Fig. 1, p. 2), with swollen bases. The knee of the sheath is prominent and the flagellum is up to 3 m long. The leaf is ecirrate, and without a petiole in mature specimens. The leaves are arcuate, about 1 m long with regularly arranged leaflets that are bristly on both margins. The male inflorescence has slightly recurved rachillae and is branched to 3 orders (Fig.
    [Show full text]
  • The Evolution and Domestication Genetics of the Mango Genus
    Florida International University FIU Digital Commons FIU Electronic Theses and Dissertations University Graduate School 4-27-2018 The volutE ion and Domestication Genetics of the Mango Genus, Mangifera (Anacardiaceae) Emily Warschefsky Florida International University, [email protected] DOI: 10.25148/etd.FIDC006564 Follow this and additional works at: https://digitalcommons.fiu.edu/etd Part of the Biodiversity Commons, Biology Commons, Botany Commons, Genetics and Genomics Commons, and the Plant Breeding and Genetics Commons Recommended Citation Warschefsky, Emily, "The vE olution and Domestication Genetics of the Mango Genus, Mangifera (Anacardiaceae)" (2018). FIU Electronic Theses and Dissertations. 3824. https://digitalcommons.fiu.edu/etd/3824 This work is brought to you for free and open access by the University Graduate School at FIU Digital Commons. It has been accepted for inclusion in FIU Electronic Theses and Dissertations by an authorized administrator of FIU Digital Commons. For more information, please contact [email protected]. FLORIDA INTERNATIONAL UNIVERSITY Miami, Florida EVOLUTION AND DOMESTICATION GENETICS OF THE MANGO GENUS, MANGIFERA (ANACARDIACEAE) A dissertation submitted in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY in BIOLOGY by Emily Warschefsky 2018 To: Dean Michael R. Heithaus College of Arts, Sciences and Education This dissertation, written by Emily Warschefsky, and entitled Evolution and Domestication Genetics of the Mango Genus, Mangifera (Anacardiaceae), having been approved
    [Show full text]
  • Andaman & Nicobar Islands, India
    RESEARCH Vol. 21, Issue 68, 2020 RESEARCH ARTICLE ISSN 2319–5746 EISSN 2319–5754 Species Floristic Diversity and Analysis of South Andaman Islands (South Andaman District), Andaman & Nicobar Islands, India Mudavath Chennakesavulu Naik1, Lal Ji Singh1, Ganeshaiah KN2 1Botanical Survey of India, Andaman & Nicobar Regional Centre, Port Blair-744102, Andaman & Nicobar Islands, India 2Dept of Forestry and Environmental Sciences, School of Ecology and Conservation, G.K.V.K, UASB, Bangalore-560065, India Corresponding author: Botanical Survey of India, Andaman & Nicobar Regional Centre, Port Blair-744102, Andaman & Nicobar Islands, India Email: [email protected] Article History Received: 01 October 2020 Accepted: 17 November 2020 Published: November 2020 Citation Mudavath Chennakesavulu Naik, Lal Ji Singh, Ganeshaiah KN. Floristic Diversity and Analysis of South Andaman Islands (South Andaman District), Andaman & Nicobar Islands, India. Species, 2020, 21(68), 343-409 Publication License This work is licensed under a Creative Commons Attribution 4.0 International License. General Note Article is recommended to print as color digital version in recycled paper. ABSTRACT After 7 years of intensive explorations during 2013-2020 in South Andaman Islands, we recorded a total of 1376 wild and naturalized vascular plant taxa representing 1364 species belonging to 701 genera and 153 families, of which 95% of the taxa are based on primary collections. Of the 319 endemic species of Andaman and Nicobar Islands, 111 species are located in South Andaman Islands and 35 of them strict endemics to this region. 343 Page Key words: Vascular Plant Diversity, Floristic Analysis, Endemcity. © 2020 Discovery Publication. All Rights Reserved. www.discoveryjournals.org OPEN ACCESS RESEARCH ARTICLE 1.
    [Show full text]
  • Reinwardtia a Journal on Taxonomic Botany, Plant Sociology and Ecology
    REINWARDTIA A JOURNAL ON TAXONOMIC BOTANY, PLANT SOCIOLOGY AND ECOLOGY ISSN 0034 – 365 X | E-ISSN 2337 − 8824 | Accredited 792/AU3/P2MI-LIPI/04/2016 2017 16 (2) REINWARDTIA A JOURNAL ON TAXONOMIC BOTANY, PLANT SOCIOLOGY AND ECOLOGY Vol. 16 (2): 49 – 110, December 19, 2017 Chief Editor Kartini Kramadibrata (Mycologist, Herbarium Bogoriense, Indonesia) Editors Dedy Darnaedi (Taxonomist, Herbarium Bogoriense, Indonesia) Tukirin Partomihardjo (Ecologist, Herbarium Bogoriense, Indonesia) Joeni Setijo Rahajoe (Ecologist, Herbarium Bogoriense, Indonesia) Marlina Ardiyani (Taxonomist, Herbarium Bogoriense, Indonesia) Himmah Rustiami (Taxonomist, Herbarium Bogoriense, Indonesia) Lulut Dwi Sulistyaningsih (Taxonomist, Herbarium Bogoriense, Indonesia) Topik Hidayat (Taxonomist, Indonesia University of Education, Indonesia) Eizi Suzuki (Ecologist, Kagoshima University, Japan) Jun Wen (Taxonomist, Smithsonian Natural History Museum, USA) Barry J. Conn (Taxonomist, School of Life and Environmental Sciences, The University of Sydney, Australia) David G. Frodin (Taxonomist, Royal Botanic Gardens, Kew, United Kingdom) Graham Eagleton (Wagstaffe, NSW, Australia) Secretary Rina Munazar Layout Liana Astuti Illustrators Subari Wahyudi Santoso Anne Kusumawaty Correspondence on editorial matters and subscriptions for Reinwardtia should be addressed to: HERBARIUM BOGORIENSE, BOTANY DIVISION, RESEARCH CENTER FOR BIOLOGY– INDONESIAN INSTITUTE OF SCIENCES CIBINONG SCIENCE CENTER, JLN. RAYA JAKARTA – BOGOR KM 46, CIBINONG 16911, P.O. Box 25 CIBINONG INDONESIA PHONE (+62) 21 8765066; Fax (+62) 21 8765062 E-MAIL: [email protected] http://e-journal.biologi.lipi.go.id/index.php/reinwardtia Cover images: Plant and flower of Appendicula cordata Wibowo & Juswara. Photos by A. R. U. Wibowo. The Editors would like to thank all reviewers of volume 16(2): Aida Baja-Lapis - Ecosystems Research and Development Bureau College, Laguna, Philippines Andre Schuiteman - Herbarium Kewense, Royal Botanic Gardens Kew, Richmond, Surrey, England, UK Eduard F.
    [Show full text]
  • (Garcinia Mangostana L.) and ITS RELATIVES BASED on MORPHOLOGICAL and INTER SIMPLE SEQUENCE REPEAT (ISSR) MARKERS
    RESEARCH ARTICLE SABRAO Journal of Breeding and Genetics 45 (3) 478-490, 2013 PHYLOGENETIC ANALYSIS OF MANGOSTEEN (Garcinia mangostana L.) AND ITS RELATIVES BASED ON MORPHOLOGICAL AND INTER SIMPLE SEQUENCE REPEAT (ISSR) MARKERS SULASSIH1, SOBIR2* and SANTOSA E2 1Center for Tropical Horticulture Studies, Bogor Agricultural University, Jl Pajajaran Baranangsiang, Bogor, Indonesia 2Department of Agronomy and Horticulture, Faculty of Agriculture at Bogor Agricultural University and Center for Tropical Horticulture Studies, Bogor Agricultural University, Jl Pajajaran Baranangsiang, Bogor, Indonesia *Corresponding author’s email: [email protected] SUMMARY Mangosteen and its relatives within the genus Garcinia L. belong to the family Guttiferae that contains about 35 genera and up to 800 species. Guttiferae diversity is found across the Indonesian archipelago. In order to elucidate the genetic diversity of mangosteen and its relatives, morphological and molecular analyses were conducted. The objectives for this study were: (1) to determine the relationships between mangosteen and its relatives; and (2) to confirm the true diversity of allotetraploid mangosteen relatives G. mangostana. Analysis was conducted using morphological and inter simple sequence repeat (ISSR) between 19 accessions of G. mangostana and their close relatives revealed. Diversity analysis was based on 212 polymorphic characters and 3 groups were formed. Group A consisted of Garcinia mangostana, Garcinia malaccensis, Garcinia celebica, Garcinia hombroniana and Garcinia porrecta; group B comprised Garcinia forbesii and Garcinia subellptica; and group C solely with Calophyllum inophyllum... The genetic similarity of Garcinia mangostana, Garcinia malaccensis and Garcinia celebica were 0.78 and 0.63. The epidermis cell observations around stomata cells on the lower surface of leaves revealed that Garcinia mangostana has the intermediate shape between Garcinia celebica and Garcinia malaccensis.
    [Show full text]
  • ALAA TAHA YASIR AL KANAN Hj.Pdf
    MECHANISMS OF ANTI-PROLIFERTATIVE EFFECT OF GARCINIA HOMBRONIANA ESSENTIAL OILS LEAVES IN MCF-7 AND MCF-7/TAMR-1 HUMAN BREAST CANCER CELL LINES BY ALAA TAHA YASIR AL KANAN DISSERTATION SUBMITTED IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE (HEALTH TOXICOLOGY) ADVANCED MEDICAL AND DENTAL INSTITUTE UNIVERSITI SAINS MALAYSIA 2019 DECLARATION I hereby declare that this research was sent to universiti sains malaysia (USM) for the degree of Master of Science in Health Toxicology. It has not been sent to other Universities. With that, this research can be used for the consultation and can be photocopied as reference. Sincerely, ------------------------- ALAA TAHA YASIR AL KANAN (P-IPM0060/18) ACKNOWLEDGEMENT In the name of Allah, the Most Beneficient, the Most Merciful. Praise to Allah, who has given me the opportunity, strength, knowledge and ability to complete this work and my Master study. Firstly, I would like to thank and show my deepest appreciation to my supervisor Dr Nik Nur Syazni Nik Mohd Kamal for her unflagging enthusiasm, valuable guidance and constant encouragement throughout the tenure of my study in IPPT, USM. Her valuable knowledge and her logical way of thinking have been of great value for me. I would also like to express gratitude to my co-supervisor Dr Tan Wen Nee, who help me with the essential oil extraction as the first step of my laboratory work and I also appreciate the support from PhD candidates, Nurul Izzati and Musthahimah Muhamad who always assist me in this research. My acknowledgement would be incomplete without my deepest thanks to the pillar of my strength, my wife and the blessings of my mother who helped me at every stage of my personal and academic life and longed to see this achievement come true.
    [Show full text]
  • Karakteristik Morfologi Dan Anatomi Daun Genus Garcinia Dataran Tinggi
    PROS SEM NAS MASY BIODIV INDON Volume 3, Nomor 3, Desember 2017 ISSN: 2407-8050 Halaman: 407-411 DOI: 10.13057/psnmbi/m030319 Karakteristik morfologi dan anatomi daun genus Garcinia dataran tinggi Characteristics of morphology and anatomy of montane Garcinia leaves MASFIRO LAILATI UPT Balai Konservasi Tumbuhan Kebun Raya Cibodas, Lembaga Ilmu Pengetahuan Indonesia. PO Box 19, Sindanglaya, Cianjur 43253, Jawa Barat. Tel.: +62-263-512233, 520448; Fax.: +62-263-512233, ♥email: [email protected], [email protected] Manuskrip diterima: 25 Agustus 2017. Revisi disetujui: 27 Desember 2017. Abstrak. Lailati M. 2017. Karakteristik morfologi dan anatomi daun genus Garcinia dataran tinggi. Pros Sem Nas Masy Biodiv Indon 3: 407-411. Garcinia merupakan genus tumbuhan yang termasuk dalam suku Clusiaceae. Ada sekitar 100 jenis Garcinia yang tersebar di Indonesia. Sebagian besar Garcinia tersebar dan tumbuh di dataran rendah dan hanya sedikit yang tumbuh di dataran tinggi. Tercatat sekitar seperlima dari jumlah Garcinia di Indonesia merupakan jenis Garcinia dataran tinggi. Tujuan dari penelitian ini adalah sebagai tahap awal untuk mengetahui karakteristik morfologi dan anatomi daun koleksi Garcinia spp. dataran tinggi di Kebun Raya Cibodas (KRC). Metode penelitian yang digunakan adalah observasi langsung di lapangan, pengamatan di laboratorium, dan studi literatur. Hasil penelitian menunjukkan terdapat sepuluh jenis Garcinia koleksi KRC dari kegiatan eksplorasi di Indonesia yang diamati. Jenis tersebut adalah G. beccarii, G. burkillii, G. celebica, G. dulcis, G. havilandii, G. horsfieldiana, G. lateriflora, G. latissima, G. Mangostana, dan G. rostrata. Karakter morfologi dan anatomi pada daun Garcinia berbeda pada tiap jenisnya. Karakter morfologi berbeda pada circumscriptio, morfometri, dan arsitektur internal daun serta memiliki tipe stomata paracytic dan anomocytic.
    [Show full text]
  • Proceedings of the International Symposium on TROPICAL PEATLANDS Bogor, Indonesia, 22-23 November 1999 Hokkaido University & Indonesian Institute of Sciences Pp
    Part 4 Forest Ecosystem Proceedings of the International Symposium on TROPICAL PEATLANDS Bogor, Indonesia, 22-23 November 1999 Hokkaido University & Indonesian Institute of Sciences pp. 153-164 (2000) Floristic Composition of Peat Swamp Forest in Mensemat-Sambas, West Kalimantan Mustaid SIREGAR and Edy Nasriadi SAMBAS Research and Development Center for Biology, The Indonesian Institute of Sciences Jalan Juanda 22, Bogor 16122, Indonesia, E-mail: [email protected] Abstract The floristic composition of a 1.05 ha (70 m by 150 m) plot in an old-growth peat swamp forest was studied at Mensemat, Sambas Regency, West Kalimantan. Number of species and density of trees with more than 10 cm in diameter was 86 species and 698 trees/ha, while small tree of 2-10 cm in diameter was 100 species and 5043 trees/ha, respectively. The most abundant trees were Blumeodendron elatriospermum (47 trees/ha), Cyatocalyx biovulatus (39 trees/ha), Blumeodendron tokbrai (32 trees/ha), Lithocarpus encleisacarpus (30 trees/ha) and Syzygium chlorantha (30 trees/ha) with greater number of individual at smaller diameter classes. The most diverse families are Euphorbiaceae, Annonaceae and Dipterocarpaceae represented mostly by small-sized trees ranging between diameter 2-30 cm and rarely exceeding dbh 60 cm. Key words: peat swamp forest, floristic composition, Mensemat, West Kalimantan. Introduction Most of the peat swamp forest around Sambas Regency; West Kalimantan has been disturbed due to land conversion and logging. Some of the disturbed peat swamp areas were drained by artificial canals and have been converted into agricultural land, such as for rubber, coffee, pineapple and other fruits plants.
    [Show full text]
  • From Barcoding to Metagenomics: Molecular Identification Techniques for Ecological Studies of Endangered Primates Amrita Srivath
    FROM BARCODING TO METAGENOMICS: MOLECULAR IDENTIFICATION TECHNIQUES FOR ECOLOGICAL STUDIES OF ENDANGERED PRIMATES AMRITA SRIVATHSAN B. Sc. (Hons.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE AND DEPARTMENT OF LIFE SCIENCES IMPERIAL COLLEGE LONDON 2014 DECLARATION I hereby declare that this thesis is my original work. I have duly acknowledged all the sources of information which have been used in the thesis. The copyright of this thesis rests with the author and is made available under a Creative Commons Attribution Non-Commercial No Derivatives licence. Researchers are free to copy, distribute or transmit the thesis on the condition that they attribute it, that they do not use it for commercial purposes and that they do not alter, transform or build upon it. For any reuse or redistribution, researchers must make clear to others the licence terms of this work ___________________ Amrita Srivathsan 31 July 2014 ii “I asked the question for the best reason possible, for the only reason, indeed that excuses anyone for asking any question - simple curiosity.” – Oscar Wilde iii ACKNOWLEDGEMENTS My most sincere gratitude goes to a number of people whose contributions were invaluable during my PhD studies: Prof. Rudolf Meier, it seems only fitting to begin this with Wilde, who, through you, had a heavy hand in the first part of the thesis. Thank you. For nurturing scientific thinking in me, for being the most supportive supervisor one can imagine, and for a lot of reasons that would be difficult to list. Your immense knowledge and ideas in many different aspects of this field lead to discussions that give me perspective on the questions we are asking and always leave me instigated about research.
    [Show full text]