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Molecular Virology and Pathogenesis (MICR 812)

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Molecular Virology and Pathogenesis (MICR 812) Microbiology KU Medical Center Molecular Virology and Pathogenesis (MICR 812) Location of Classes: TBD A. Contact Information Jianming Qiu, Ph.D. 4004 Hixon 588‐4329 [email protected] B. Purpose of this Course This Virology course is aimed at graduate students who pursue master degree in science in the Department of Microbiology. It provides a contemporary understanding of how viruses are built, how they infect and replicate in host cells, how they spread and evolve, how they interact with host cells, how they eventually cause diseases, and how infection of a host can be prevented. This course will provide a balanced approach to Virology, combining the molecular and pathogenesis aspects of Virology. While it is focused primarily on human viruses, it will also discuss animal viruses, as human viruses often are evolved from animal viruses. In addition to traditional topics, this course will explain new “hot” trends in Virology, including: virus‐based vector in human gene therapy; modern advances in vaccinology; “oncolytic” viruses to treat cancers; emerging viruses and potential bioterrorism agents (influenza virus, coronavirus, and filoviruses). C. Intended Course Outcomes By the end of this course, students should be able to: 1. Describe virus taxonomy, virus structure and virus entry, trafficking and egress. 2. Describe the basics of the viral gene expression, including viral replication, transcription, post‐transcriptional regulation, translation and post‐translational regulation of virus genes. 3. Apply techniques used in modern virology and design experiments to test novel hypotheses in virology. 4. Distinguish diverse characteristics of viruses – host range, target tissues, replication strategy, transmission, etc., in particular, of these emerging/reemerging viruses, e.g., influenza and Ebola viruses, and medical important viruses, e.g., HIV. 5. Describe the principle of virus infection and the host immune defense, viral ontogenesis innovative approaches to the development of antiviral drugs and vaccines. 6. Relate the principle of virus infection, the host immune response and viral ontogenesis to innovative approaches to the development of antiviral drugs and vaccines. D. Text/Reference Book: Textbook: S. J. Flint, et al: Principles of Virology: Molecular Biology, Pathogenesis, and Control of Animal Viruses (second edition, year) ASM press. Reference book: D. M. Knipe, et al: Fields Virology (6 edition, 2011) Lippincott Williams & Wilkins. E. Organization of the Course This course is designed as a set of interactive lectures. Students must read the relevant chapters in the textbook before each class. The course sessions will generally begin with readiness quiz and a lecture on a given topic, with questions to facilitate discussion. This will be followed by a quiz at the end of the session. F. Expectations of Participants We will meet an hour each Wednesday and Friday morning throughout the semester. You are required to complete the pre‐class assignment, attend the learning sessions, participate in the class activities and submit quizzes. Grades will be determined based on students meeting the attendance requirements, participating in a respectful and collegial manner in class, submitting quizzes, and completing the midterm and final exams. H. Assessments Learning will be assessed by oral and written assignments, including low‐stakes “readiness quizzes” during class time. Some of your graded projects will be undertaken in teams of learners. Grades will be determined by the following formula: 20% assignments/readiness quizzes/classroom participation 20% session assessments (see calendar of sessions) 30% team‐based learning projects 30% final project presentations Grading Scale: 86 – 100% = A 75 – 85% = B 65 – 74% = C 60 – 64% = D <60% = F I. Schedule 10:00 – 10:50 AM, Monday and Wednesday You MUST read the relevant textbook chapter BEFORE the scheduled lecture. Lecture Date Lecturer Topic Subtopics number 1 Mon, Aug 24 TBD Virus Taxonomy Classification of animal viruses (A chapter in a Viral genomes reference book, TBD) 2 Wed, Aug 26 Qiu Virology Methods Virus culture, detection, (A chapter in a diagnostics and genetics reference book, TBD) 3 Mon, Aug 31 TBD Virus Entry Virus attachment, receptors, and (Vol. I, Chapter 5) intracellular trafficking 4 Wed, Sept 2 TBD RNA Virus Replication Negative stranded RNA viruses (Vol. I, Chapter 6) Positive stranded RNA viruses Double stranded RNA viruses Mon, Sept 7 LABOR DAY 5 Wed, Sept 9 Qiu Viral DNA Transcription Transcription initiation (Vol. I, Chapter 8) complexes Viral transactivators 6 Mon, Sept 14 TBD Reverse Transcription Retrovirus & HIV& hepatitis B (Vol. I, Chapter 7) virus 7 Wed, Sept 16 Qiu DNA Virus Replication ds DNA viruses (Vol. I, Chapter 9) ssDNA viruses Viral DNA replication 8 Mon, Sept 21 Qiu Viral RNA processing RNA splicing, polyadenylation, (Vol. I, Chapter 10) and RNA export 9 Wed, Sept 23 Qiu Viral mRNA translation Mechanism of translation (Vol. I, Chapter 11) 10 Mon, Sept 28 Qiu ASSESSMENT #1 11 Wed, Sept 30 TBD Virus Assembly Virus assembly, maturation, and (Vol. I, Chapter 13) release 12 Mon, Oct 5 TBD Principles of virus Infection of a host, infection of infection populations (Vol. II, Chapter 1&2) 13 Wed Oct 7 Qiu Host defense II Innate immune response against (Vol. II, Chapter 4) DNA viruses 14 Mon, Oct 12 Qiu Host defense III Innate immune response against (Vol. II, Chapter 4) RNA viruses 15 Wed, Oct 14 TBD Infection patterns I Lytic, latent infection, and slow (Vol. II, Chapter 5) infections 16 Mon, Oct 19 TBD Infection patterns II Transformation and ocogenesis (Vol. II, Chapter 7) 17 Wed, Oct 21 Qiu ASSESSMENT #2 18 Mon, Oct 26 TBD Vaccine Vaccine development and the (Vol. II, Chapter 8) challenges 19 Wed, Oct 28 TBD Antiviral drugs I Principle of antiviral drug (Vol. II, Chapter 9) development 20 Mon, Nov 2 TBD Antiviral drugs Antiviral drug development, and (Vol. II, Chapter 9) anti‐HIV and anti‐HCV drugs 21 Wed, Nov 4 Qiu Viral Vector I DNA virus vector (Ad, AAV, and (A chapter in a HSV vectors) reference book, TBD) 22 Mon, Nov 9 Qiu Viral Vector II RNA virus vector (A chapter in a (Retroviral/lentiviral Vector and reference book, TBD) other RNA viral vectors) 23 Wed, Nov 11 Qiu ASSESSMENT #3 24 Mon, Nov 16 TBD Emerging viruses I Influenza virus (A chapter in a reference book, TBD) 25 Wed, Nov 18 TBD Emerging viruses II Ebola virus (A chapter in a reference book, TBD) 26 Mon, Nov 23 TBD Emerging viruses III Coronavirus (A chapter in a reference book, TBD) Wed, Nov 25 NO CLASSES 27 Mon, Nov 30 TBD Topics in virology I HIV pathogenesis (Vol. II, Chapter 6) 28 Wed, Dec 2 TBD Topics in virology II HBV, HCV, HEV and HDV (A chapter in a reference book, TBD) 29 Mon, Dec 7 Qiu Topics in virology IV Small DNA viruses (A chapter in a reference book, TBD) 30 Wed, Dec 9 Qiu ASSESSMENT #4 31 Mon, Dec 14 Qiu Final exam Final project presentations 32 Wed, Dec 16 Qiu Final exam Final project presentations .
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