ChemBioEng Reviews Anaerobic radical enzymes for biotechnology Journal: ChemBioEng Reviews Manuscript ID cben.201800003.R1 Wiley - Manuscript type:For Review Peer Review Date Submitted by the Author: n/a Complete List of Authors: Jäger, Christof; University of Nottingham, Chemical and Environmental Engineering Croft, Anna; University of Nottingham, Chemical and Environmental Engineering Keywords: Radicals, Enzymes, Catalysis, Biotechnology, Anaerobic reactions Wiley-VCH Page 1 of 61 ChemBioEng Reviews 1 2 3 Christof M. Jäger* and Anna K. Croft* 4 5 6 7 8 9 Anaerobic radical enzymes for biotechnology 10 11 12 13 AUTHORS: Dr Christof Martin Jäger* and Dr Anna Kristina Croft* 14 15 16 ADDRESS: Department of Chemical and Environmental Engineering, University of 17 18 Nottingham, Nottingham, NG7 2RD, United Kingdom.
[email protected], 19 For Peer Review 20 21
[email protected] 22 23 24 ABSTRACT: 25 26 27 28 Enzymes that proceed through radical intermediates have a rich chemistry that includes 29 30 functionalisation of otherwise unreactive carbon atoms, carbon-skeleton rearrangements, 31 32 aromatic reductions, and unusual eliminations. Especially under anaerobic conditions, 33 34 organisms have developed a wide range of approaches for managing these transformations 35 36 that can be exploited to generate new biological routes towards both bulk and specialty 37 38 39 chemicals. These routes are often either much more direct or allow access to molecules that 40 41 are inaccessible through standard (bio)chemical approaches. This review gives an overview 42 43 of some of the key enzymes in this area: benzoyl-CoA reductases (that effect the enzymatic 44 45 Birch reduction), ketyl radical dehydratases, coenzyme B12-dependant enzymes, glycyl 46 47 radical enzymes, and radical SAM (AdoMet radical) enzymes.