NFYC Polyclonal Antibody

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For Research Use Only NFYC Polyclonal antibody Catalog Number:10129-2-AP 1 Publications www.ptgcn.com Catalog Number: GenBank Accession Number: Recommended Dilutions: Basic Information 10129-2-AP BC005003 WB 1:500-1:2000 Size: GeneID (NCBI): 470 μg/ml 4802 Source: Full Name: Rabbit nuclear transcription factor Y, gamma Isotype: Calculated MW: IgG 37 kDa, 50 kDa Purification Method: Observed MW: Antigen affinity purification 48 kDa Immunogen Catalog Number: AG0140 Applications Tested Applications: Positive Controls: WB,ELISA WB : K-562 cells; Cited Applications: WB Species Specificity: human, mouse, rat Cited Species: mouse NFYC, also named as Nuclear transcription factor Y subunit gamma or Transactivator HSM-1/2, is a 458 amino acid Background Information protein, which belongs to the NFYC/HAP5 subunit family. NFYC localizes in the nucleus and stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. SP1, NFYC and FOXO3, FOXO4 transcription factors are involved in the regulation of STK11 transcription. Notable Publications Author Pubmed ID Journal Application Tong Zan Z 19365404 Cell Res WB Storage: Storage Store at -20ºC. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 4006900926 E: [email protected] W: ptgcn.com Group brand and is not available to purchase from any other manufacturer. Selected Validation Data K-562 cells were subjected to SDS PAGE followed by western blot with 10129-2-AP (NFYC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours..

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Anti-NFYC Antibody (ARG66549)
Product datasheet [email protected] ARG66549 Package: 100 μl anti-NFYC antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes NFYC Tested Reactivity Hu, Ms Tested Application IHC-P, WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name NFYC Antigen Species Human Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of Human NFYC. Conjugation Un-conjugated Alternate Names HAP5; NF-YC; Nuclear transcription factor Y subunit gamma; Nuclear transcription factor Y subunit C; CBF-C; HSM; CBFC; Transactivator HSM-1/2; CAAT box DNA-binding protein subunit C; H1TF2A Application Instructions Application table Application Dilution IHC-P 1:100 - 1:200 WB 1:500 - 1:1000 Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Calculated Mw 50 kDa Observed Size 50 kDa Properties Form Liquid Purification Affinity purification with immunogen. Buffer 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. Preservative 0.01% Sodium azide Stabilizer 30% Glycerol Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/2 Note For laboratory research only, not for drug, diagnostic or other use.
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Molecular Mechanisms for Transcriptional Regulation of Human High-Affinity IgE Receptor β-Chain Gene Induced by GM-CSF This information is current as Kyoko Takahashi, Natsuko Hayashi, Shuichi Kaminogawa of September 23, 2021. and Chisei Ra J Immunol 2006; 177:4605-4611; ; doi: 10.4049/jimmunol.177.7.4605 http://www.jimmunol.org/content/177/7/4605 Downloaded from References This article cites 39 articles, 16 of which you can access for free at: http://www.jimmunol.org/content/177/7/4605.full#ref-list-1 http://www.jimmunol.org/ Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication by guest on September 23, 2021 *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2006 by The American Association of Immunologists All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. The Journal of Immunology Molecular Mechanisms for Transcriptional Regulation of Human High-Afﬁnity IgE Receptor ␤-Chain Gene Induced by GM-CSF1 Kyoko Takahashi,*† Natsuko Hayashi,*‡ Shuichi Kaminogawa,† and Chisei Ra2* The ␤-chain of the high-afﬁnity receptor for IgE (Fc␧RI) plays an important role in regulating activation of Fc␧RI-expressing cells such as mast cells in allergic reactions.
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Roschger and Cabrele Cell Communication and Signaling (2017) 15:7 DOI 10.1186/s12964-016-0161-y REVIEW Open Access The Id-protein family in developmental and cancer-associated pathways Cornelia Roschger and Chiara Cabrele* Abstract Inhibitors of DNA binding and cell differentiation (Id) proteins are members of the large family of the helix-loop- helix (HLH) transcription factors, but they lack any DNA-binding motif. During development, the Id proteins play a key role in the regulation of cell-cycle progression and cell differentiation by modulating different cell-cycle regulators both by direct and indirect mechanisms. Several Id-protein interacting partners have been identified thus far, which belong to structurally and functionally unrelated families, including, among others, the class I and II bHLH transcription factors, the retinoblastoma protein and related pocket proteins, the paired-box transcription factors, and the S5a subunit of the 26 S proteasome. Although the HLH domain of the Id proteins is involved in most of their protein-protein interaction events, additional motifs located in their N-terminal and C-terminal regions are required for the recognition of diverse protein partners. The ability of the Id proteins to interact with structurally different proteins is likely to arise from their conformational flexibility: indeed, these proteins contain intrinsically disordered regions that, in the case of the HLH region, undergo folding upon self- or heteroassociation. Besides their crucial role for cell-fate determination and cell-cycle progression during development, other important cellular events have been related to the Id-protein expression in a number of pathologies. Dysregulated Id-protein expression has been associated with tumor growth, vascularization, invasiveness, metastasis, chemoresistance and stemness, as well as with various developmental defects and diseases.
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Supplementary Table 2
Supplementary Table 2. Differentially Expressed Genes following Sham treatment relative to Untreated Controls Fold Change Accession Name Symbol 3 h 12 h NM_013121 CD28 antigen Cd28 12.82 BG665360 FMS-like tyrosine kinase 1 Flt1 9.63 NM_012701 Adrenergic receptor, beta 1 Adrb1 8.24 0.46 U20796 Nuclear receptor subfamily 1, group D, member 2 Nr1d2 7.22 NM_017116 Calpain 2 Capn2 6.41 BE097282 Guanine nucleotide binding protein, alpha 12 Gna12 6.21 NM_053328 Basic helix-loop-helix domain containing, class B2 Bhlhb2 5.79 NM_053831 Guanylate cyclase 2f Gucy2f 5.71 AW251703 Tumor necrosis factor receptor superfamily, member 12a Tnfrsf12a 5.57 NM_021691 Twist homolog 2 (Drosophila) Twist2 5.42 NM_133550 Fc receptor, IgE, low affinity II, alpha polypeptide Fcer2a 4.93 NM_031120 Signal sequence receptor, gamma Ssr3 4.84 NM_053544 Secreted frizzled-related protein 4 Sfrp4 4.73 NM_053910 Pleckstrin homology, Sec7 and coiled/coil domains 1 Pscd1 4.69 BE113233 Suppressor of cytokine signaling 2 Socs2 4.68 NM_053949 Potassium voltage-gated channel, subfamily H (eag- Kcnh2 4.60 related), member 2 NM_017305 Glutamate cysteine ligase, modifier subunit Gclm 4.59 NM_017309 Protein phospatase 3, regulatory subunit B, alpha Ppp3r1 4.54 isoform,type 1 NM_012765 5-hydroxytryptamine (serotonin) receptor 2C Htr2c 4.46 NM_017218 V-erb-b2 erythroblastic leukemia viral oncogene homolog Erbb3 4.42 3 (avian) AW918369 Zinc finger protein 191 Zfp191 4.38 NM_031034 Guanine nucleotide binding protein, alpha 12 Gna12 4.38 NM_017020 Interleukin 6 receptor Il6r 4.37 AJ002942
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Hindawi Complexity Volume 2017, Article ID 9194801, 8 pages https://doi.org/10.1155/2017/9194801 Research Article Identifying the Risky SNP of Osteoporosis with ID3-PEP Decision Tree Algorithm Jincai Yang,1 Huichao Gu,1 Xingpeng Jiang,1 Qingyang Huang,2 Xiaohua Hu,1 and Xianjun Shen1 1 School of Computer Science, Central China Normal University, Wuhan 430079, China 2School of Life Science, Central China Normal University, Wuhan 430079, China Correspondence should be addressed to Jincai Yang; [email protected] Received 31 March 2017; Revised 26 May 2017; Accepted 8 June 2017; Published 7 August 2017 Academic Editor: Fang-Xiang Wu Copyright © 2017 Jincai Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. In the past 20 years, much progress has been made on the genetic analysis of osteoporosis. A number of genes and SNPs associated with osteoporosis have been found through GWAS method. In this paper, we intend to identify the suspected risky SNPs of osteoporosis with computational methods based on the known osteoporosis GWAS-associated SNPs. The process includes two steps. Firstly, we decided whether the genes associated with the suspected risky SNPs are associated with osteoporosis by using random walk algorithm on the PPI network of osteoporosis GWAS-associated genes and the genes associated with the suspected risky SNPs. In order to solve the overfitting problem in ID3 decision tree algorithm, we then classified the SNPs with positive results based on their features of position and function through a simplified classification decision tree which was constructed by ID3 decision tree algorithm with PEP (Pessimistic-Error Pruning).
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523.Full-Text.Pdf
ANTICANCER RESEARCH 36: 523-532 (2016) Molecular Pathways Mediating Metastases to the Brain via Epithelial-to-Mesenchymal Transition: Genes, Proteins, and Functional Analysis DHRUVE S. JEEVAN1, JARED B. COOPER1, ALEX BRAUN2, RAJ MURALI1 and MEENA JHANWAR-UNIYAL1 Departments of 1Neurosurgery and 2Pathology, New York Medical College, Valhalla, NY, U.S.A. Abstract. Background: Brain metastases are the leading expressed. Moreover, co-expression of the epithelial marker E- cause of morbidity and mortality among patients with cadherin with the mesenchymal marker vimentin was evident, disseminated cancer. The development of metastatic disease suggesting a state of transition. Expression analysis of involves an orderly sequence of steps enabling tumor cells to transcription factor genes in metastatic brain tumor samples migrate from the primary tumor and colonize at secondary demonstrated an alteration in genes associated with locations. In order to achieve this complex metastatic potential, neurogenesis, differentiation, and reprogramming. a cancer cell is believed to undergo a cellular reprogramming Furthermore, tumor cells grown in astrocytic medium displayed process involving the development of a degree of stemness, via increased cell proliferation and enhanced S-phase cell-cycle a proposed process termed epithelial-to-mesenchymal entry. Additionally, chemotactic signaling from the astrocytic transition (EMT). Upon reaching its secondary site, these environment promoted tumor cell migration. Primary tumor reprogrammed cancer stem cells submit to a reversal process cells and astrocytes were also shown to grow amicably designated mesenchymal-to-epithelial transition (MET), together, forming cell-to-cell interactions. Conclusion: These enabling establishment of metastases. Here, we examined the findings suggest that cellular reprogramming via EMT/MET expression of markers of EMT, MET, and stem cells in plays a critical step in the formation of brain metastases, where metastatic brain tumor samples.
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NFYC Antibody Cat
NFYC Antibody Cat. No.: 58-221 NFYC Antibody Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat This NFYC antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 100-129 amino acids from the N-terminal region of human NFYC. TESTED APPLICATIONS: WB APPLICATIONS: For WB starting dilution is: 1:1000 PREDICTED MOLECULAR 50 kDa WEIGHT: Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig CONJUGATE: Unconjugated September 29, 2021 1 https://www.prosci-inc.com/nfyc-antibody-58-221.html PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: NFYC Nuclear transcription factor Y subunit gamma, CAAT box DNA-binding protein subunit C, ALTERNATE NAMES: Nuclear transcription factor Y subunit C, NF-YC, Transactivator HSM-1/2, NFYC ACCESSION NO.: Q13952 PROTEIN GI NO.: 20137773 GENE ID: 4802 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References This gene encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein, subunit C, forms a tight dimer with the B subunit, a BACKGROUND: prerequisite for subunit A association.
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NFYC Antibody - C-Terminal Region Rabbit Polyclonal Antibody Catalog # AI16112
10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 NFYC Antibody - C-terminal region Rabbit Polyclonal Antibody Catalog # AI16112 Specification NFYC Antibody - C-terminal region - Product Information Application WB Primary Accession Q13952 Reactivity Human Host Rabbit Clonality Polyclonal Calculated MW 50kDa KDa NFYC Antibody - C-terminal region - Additional Information Host: Rabbit Gene ID 4802 Target Name: NFYC Sample Tissue: Hela Whole Cell lysates Alias Symbol NFYC, Antibody Dilution: 1.0μg/ml Other Names Nuclear transcription factor Y subunit gamma, CAAT box DNA-binding protein NFYC Antibody - C-terminal region - subunit C, Nuclear transcription factor Y Background subunit C, NF-YC, Transactivator HSM-1/2, NFYC Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which Format specifically recognizes a 5'- CCAAT-3' box motif Liquid. Purified antibody supplied in 1x PBS found in the promoters of its target genes. NF- buffer with 0.09% (w/v) sodium azide and Y can function as both an activator and a 2% sucrose. repressor, depending on its interacting cofactors. Reconstitution & Storage Add 50 &mu, l of distilled water. Final NFYC Antibody - C-terminal region - Anti-NFYC antibody concentration is 1 References mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. Nakshatri H.,et al.J. Biol. Chem. 271:28784-28791(1996). Precautions Bellorini M.,et al.Gene 193:119-125(1997). NFYC Antibody - C-terminal region is for Dmitrenko V.V.,et al.Gene 197:161-163(1997). research use only and not for use in Taira T.,et al.J.
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Molecular Targeting and Enhancing Anticancer Efficacy of Oncolytic HSV-1 to Midkine Expressing Tumors
University of Cincinnati Date: 12/20/2010 I, Arturo R Maldonado , hereby submit this original work as part of the requirements for the degree of Doctor of Philosophy in Developmental Biology. It is entitled: Molecular Targeting and Enhancing Anticancer Efficacy of Oncolytic HSV-1 to Midkine Expressing Tumors Student's name: Arturo R Maldonado This work and its defense approved by: Committee chair: Jeffrey Whitsett Committee member: Timothy Crombleholme, MD Committee member: Dan Wiginton, PhD Committee member: Rhonda Cardin, PhD Committee member: Tim Cripe 1297 Last Printed:1/11/2011 Document Of Defense Form Molecular Targeting and Enhancing Anticancer Efficacy of Oncolytic HSV-1 to Midkine Expressing Tumors A dissertation submitted to the Graduate School of the University of Cincinnati College of Medicine in partial fulfillment of the requirements for the degree of DOCTORATE OF PHILOSOPHY (PH.D.) in the Division of Molecular & Developmental Biology 2010 By Arturo Rafael Maldonado B.A., University of Miami, Coral Gables, Florida June 1993 M.D., New Jersey Medical School, Newark, New Jersey June 1999 Committee Chair: Jeffrey A. Whitsett, M.D. Advisor: Timothy M. Crombleholme, M.D. Timothy P. Cripe, M.D. Ph.D. Dan Wiginton, Ph.D. Rhonda D. Cardin, Ph.D. ABSTRACT Since 1999, cancer has surpassed heart disease as the number one cause of death in the US for people under the age of 85. Malignant Peripheral Nerve Sheath Tumor (MPNST), a common malignancy in patients with Neurofibromatosis, and colorectal cancer are midkine- producing tumors with high mortality rates. In vitro and preclinical xenograft models of MPNST were utilized in this dissertation to study the role of midkine (MDK), a tumor-specific gene over- expressed in these tumors and to test the efficacy of a MDK-transcriptionally targeted oncolytic HSV-1 (oHSV).
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NFYC Antibody Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP51389
10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 NFYC Antibody Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP51389 Specification NFYC Antibody - Product Information Application WB Primary Accession Q13952 Reactivity Human, Mouse, Rat Host Rabbit Clonality Polyclonal Calculated MW 50 KDa Antigen Region 11 - 70 NFYC Antibody - Additional Information Gene ID 4802 Other Names Nuclear transcription factor Y subunit gamma, CAAT box DNA-binding protein Anti-NFYC Antibodyat 1:1000 dilution + HeLa subunit C, Nuclear transcription factor Y whole cell lysates Lysates/proteins at 20 µg subunit C, NF-YC, Transactivator HSM-1/2, per lane. Secondary Goat Anti-Rabbit IgG, NFYC (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 50 kDa Target/Specificity Blocking/Dilution buffer: 5% NFDM/TBST. KLH conjugated synthetic peptide derived from human NFYC NFYC Antibody - Background Dilution WB~~ 1:1000 Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in Format promoters, for example in type 1 collagen, 0.01M PBS, pH 7.2, 0.1% Sodium azide, albumin and beta-actin genes. Glycerol 50% Storage NFYC Antibody - References Store at -20 °C.Stable for 12 months from date of receipt Nakshatri H.,et al.J. Biol. Chem. 271:28784-28791(1996). Bellorini M.,et al.Gene 193:119-125(1997). Dmitrenko V.V.,et al.Gene 197:161-163(1997). NFYC Antibody - Protein Information Taira T.,et al.J. Biol. Chem. 274:24270-24279(1999). Name NFYC Bringuier P.P.,et al.Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
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The Hormone-Bound Vitamin D Receptor Regulates Turnover of Target
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