Salih et al (2020): Antibacterial activity of Italic January 2020 Vol. 23 (I)

Antibacterial activity of Italic leaves Aqueous Extract Against Two Pathogenic Bacteria

Aml Hendi Salih1 Rajaa Hendi Salih2 Madeha H. Hussain 3 Ali S. Hassoon 4*

1,3(Department of Pharmacy, Medical Institute Tech. Mansour / Middle Technical University/ Iraq) 2(Department of Biological Science, College of Science / Mustansiriyah University, Iraq) 4(Department of Soil and Water Techniques, Al-Musaib Tech. College/ Al-Furat Al-Awsat Technical University, Iraq)

*Correspond Author: ALI S. HASSOON: [email protected], [email protected] Abstract This research was achieved to detect the antibacterial efficacy of Sativa leaves aqueous extract against Two pathogenic bacteria, Two bacterial isolates Escherichia coli and staphylococcus aureus were isolated from cases of infected wound were used in this study . The anti-bacterial efficacy of Eruca Sativa Italic leaves extract was detected by measuring the inhibition zone in millimeters in 3 plates and the average was considered. The results showed Eruca Sativa extract inhibit both bacterial spp and the mean inhibitory zone for S.aureus was 14.2 mm and for E.Coli was 9.0mm.the mean inhibitory zone of Ampicillin was 20mm for S.aureus and 21.3mm for E. coli distilled water shown no antibacterial activity.

Keywords: Eruca Sativa, leaves aqueous extract, S. aureus, E. Coli

How to cite this article: Salih AH, Salih RH, et al (202Antibacterial activity of Italic leaves aqueous extract Formatiert: Rechts against two pathogenic bacteria, Ann Trop Med & Pub Health; 23:S409. DOI: http://doi.org/10.36295/ASRO.2020.23123

Introduction Eruca sativa (syns. subspecies sativa (Miller) Thell., Brassica eruca L.) is an annual edible belongs to the family. It used as leaves for their tart , fresh , flavor , peppery , and bitter. This plant is also called garden rocket,( Flora of NW Europe: Eruca vesicaria) (South African, New Zealand, Irish, Australian, and British), and eruca. However, others names are "rocket salad "rucola", "rucoli", "rugula", "colewort", and "roquette". E. sativa, is very popular as salad , is a natively type of Eruca to countries of Mediterranean sea like Syria, Portugal, Turkey, Lebanon , and Morocco (Blamey and Grey-Wilson, 1989).

Eruca sativa is growing to height ranged from 20 to 100 cm . Its leaves has pinnate shape which have four-teen deep , small , a large terminal lobe and lateral lobes. Its ranged from two to four centimeters, orderly in a corymb in an exemplary Brassicaceae fashion, a creamy-white petal with purple veins, have a yellow stamen; the is shed shortly after opening the . The is a pod (siliqua ) ranging in length from 12-35 mm with the apical beak, and contains many (edible). This species has a number of chromosome 2n=22. (Blamey and Grey-Wilson, 1989) Griffiths and Huxley,1992).

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Salih et al (2020): Antibacterial activity of Italic leaves January 2020 Vol. 23 (I)

Some medicinal contain both a harmful and beneficial substance that can enhance and/or delay an individual's health. The basis for this is that it is important to examine and re-examine the medicinal plant to identify active, harmful, and harmless components. The herbs don’t only give us chemicals with the medicinal values, but they also give us trace elements and useful nutrients. The trace elements and minerals are the chemical materials that our bodies required for many physiological and biological processes which considered necessary to maintain the health. Minerals involve compounds of phosphorus, , sulfur, , , chlorine, and sodium. Trace elements are necessary for health of human which involve iodine, selenium, zinc, copper, iron, manganese, chromium, and molybdenum. The microorganism has the genetic capability to acquire and transfer the resistance to the antibiotic then lead to generate new main health problems in the world. This forced the scientists to seek for a new drugs from biological resources like plants (Khoobchandani,et al,2010). Anti-microbial compounds derived from plants may inhibit different bacteria through various mechanisms and give medical values to treat resistant bacteria infections ( Stein et al ., 2005 ). The herb needs to be scientifically evaluated for its antimicrobial efficacy toward antibiotic-resistant bacteria in spite of developing a new drug from phyto-sources (Simoes and Rosa, 2009).

The infection of S. aureus can transmit throughout the contact with the pus cells from the injured wounds, with skin contact with the infected persons, or with equipments of the infected persons like clothing, sheets, towels, or athletic object. Joint replacements put the persons particularly at risk of pneumonia, septic arthritis, and endocarditis. S. aureus is an important cause of the chronic biofilms infection in the medical implants, and the toxins repressor is part of the pathway of infections (Tong, et al.,2015).

S. aureus can be sleeping in human bodies for several years undetected. When the symptom begins to appear, the host becomes contagious for 2 weeks, and the total disease lasts for few weeks. The diseases can be fatal if untreated. S. aureus infections can be very severe (Masalha, et al, 2001).

Most strains of E.coli don’t lead to any disease, but the virulent strains can cause hemorrhagic colitis, neonatal meningitis, urinary tract infections, Crohn's disease, and gastroenteritis, . Common symptoms and signs involve serious abdominal hemorrhagic colitis, vomiting, diarrhea, cramps, and at times fever. Also, in unusual cases, virulent strains are answerable for necrosis of bowel (death of tissues) and perforation without progression to peritonitis, septicemia, mastitis, gram negative pneumonia, and hemolytic uremic syndrome. The very young child is more probably to develop a severe disease like hemolytic-uremic syndrome. Nevertheless, healthy peoples of all ages are with a risk of serious consequences which maybe increase as a result for infection by Escherichia coli (Vogt and Dippold, 2005).

Objective This research was conducted to detect the Antibacterial efficacy of Eruca Sativa leaves aqueous extract against Two pathogenic bacteria Material and Method

Bacterial isolates Formatiert: Schriftart: 10 Pt.

Two bacterial isolates E. coli , and S. aureus were isolated from cases of infected wound . The isolates were Formatiert: Schriftart: 10 Pt., Kursiv identified a ccording to (Collee et al 1996) . Formatiert: Schriftart: 10 Pt. Preparation of bacterial inoculum . Formatiert: Schriftart: 10 Pt., Kursiv Formatiert: Schriftart: 10 Pt.

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Salih et al (2020): Antibacterial activity of Italic leaves January 2020 Vol. 23 (I)

Single isolated colonies from each bacterial spp were inoculated Separately into BHI ( Brain Heart infusion) broth which obtained from (oxoid,UK) and then incubated at 37° C for 18 hr.

Sensitivity test

Disc diffusion method is used usually qualified by Bauer et al. (1966) Formatiert: Schriftart: 10 Pt., Kursiv 0.1 mL of each bacterial broth with approximately (1.5 x 188) CFU/mL was Streaked on Brain Heart infusion Formatiert: Schriftart: 10 Pt. (BHF) agar (oxoid UK) . sterole discs 5mm in diameter, the first containg 10ML (micro liter) of Eruca Sativa extract, the Second disc was 0.25mg Ampicillin . as a positive control . and the third containing 10 micro liter of Sterial distilled water as a negative control . Discs ware placed on BHI agar surface and then incubated for 24 hr at 37° C. The sensitivity test was done in triplicate for each bacterial spp . Results The antibacterial activity of Eruca Sativa leaves extract has been detected through measurement of zone of inhibition in millimeters (mm) in three plates , and the average was considered . Eruca Sativa extract inhibit both bacterial spp and the mean inhibition zone for S.aureus was 14.2 mm and for E. coli was 9.0mm . the mean inhibition zone of Ampicillin was 20mm for S.aureus and 21.3mm for E.coli . distilled water showed no antibacterial activity .

Table ( 1) Antibacterial activity of E.Sativa leaves aqueous extract . Inhibition zone in mm Uicroorgani SMS e.Sativa extract Positive control Negative control Staph. Aureus 14.2 20 - e. Coli 9 21.3 - - = means no inhibition zone Discussion The intact fresh plant contain several bioactive constituents with health benefit but some heat labile photochemical ware destroyed on cooking (Kim and Ishii 2006) . Our results indicate the activity of Eruca Sativa leaves aqueous extract against E. coli and S. aureus but the difference in sensitivity may be due to the cell wall structure of each organism . (Mahesh and Satish 2008) . S.aureus was more susceptible than E.coli , this result was in agreement with Entesar and Amal 2007) . When Eruca Sativa leaves ware cut , glucosinolates secondary metabolites ware released and hydrolyzed by myrosinase enzyme to from methyl – isothiocyanate, Allyl – isothiocyanate and 4-methyl sulfinyl – butyl – isothiocyanate . ( Antonious et al ., 2009 ; Vig et al ., 2009 ) . Isshiki et al ., (1992) find that Allyl – isothiocyanate posses antibacterial efficacy toward two Gram-negative and positive bacteria of ( E. coli and S. aureus ). Borges et al explain the effect of Allyl – isothiocyanate on cell integrity resulting in potassium leakage and also affect iodine uptake .

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Salih et al (2020): Antibacterial activity of Italic leaves January 2020 Vol. 23 (I)

Other secondary metabolites formed on Eruca sativa leaves cut ware Alkaloids, terpneoid, and flavonoid which were affect efflux system of the cytoplasmic membrane, synthesis and function of DNA/RNA and result in cytoplasmic organelles coagulation. (Hussein 2013, Radulovic et al 2013). Conclusion:- From this work we can conclude the Eruca sativa leaves extracts act as good antibacterial compound for both gram positive and gram negative bacteria

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