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Sensors for Biotech & Pharma Industries O² pH CO² BIOMASS Sensors for Biotech & Pharma Industries Micro-invasive or even contactless measurements For microbial & cell culture From μL to m3 scale Bioprocess development & drug screening Quality control www.PreSens.de/biotech-pharma 2 Sensors for the Biotech & Pharma Industries CO ² O ² PH Biomass Contact us and we find your customized solution! 3 Content 04 Company 05 Industries CONTENT 06 Sensor Solutions 10 Featured Systems 12 Featured Applications 14 Examples for Meters, Sensors & Accessories Functional Principle METER SENSOR ACCESSORY + + www.PreSens.de/biotech-pharma 4 Sensors for the Biotech & Pharma Industries We bring to light what‘s inside... Products Made in Germany Company Profile PreSens offers a broad range of sensor systems for Based on research activities started in the 1980‘s end users in Bioprocess Control, Biological & Environ- PreSens Precision Sensing GmbH was founded in 1997 mental Research, the Food & Beverage industry as as a spin-off from the University of Regensburg, Germany. well as other industrial applications. The company combines long-time experiences of different re - searchers in the fields of electronic engineering and sensor de- We offer systems for velopment. Right from the beginning, microsensor systems were Oxygen measurement in gases and liquids sold to customers in life sciences. Already in its first decade of Non-invasive online pH, CO and oxygen measurement 2 operation PreSens became one of the leading companies in the Oxygen and pH sensors for single-use bioreactors field of chemical optical sensor technology. Together with its Microsensors pH, oxygen and CO 2 partners it offers full service in Europe, America and Asia. Process control in shake flasks incl. biomass monitoring Low-maintenance DO measurement for fermentation and bioreactor systems Service Online oxygen and pH measurement in disposables like multiwell plates and plastic bags Furthermore, we are developers and manufacturers of opto- Imaging solutions for 2D-mapping of oxygen-, pH-, electronic OEM sensor components for companies in the field of medical equipment and process control. and CO2-distribution Our product range is constantly expanding. Quality Management ISO 9001 ISO 13485 Voluntary participation in regular monitoring Contact us and we find your customized solution! 5 ...and work for the following industries. Biotech & Pharma Our Biotech & Pharma business field helps pharmaceutical com- COMPANY & INDUSTRIES COMPANY panies such as Roche and DSM to improve their bioprocess devel- Research Brewery Bottling opmentQuality with Control PreSens sensors. With two decades of customer feedback our product development provides efficient solutions for your needs. Food & Beverage A cooperation with the market leader for beverage filling systems, Krones AG, Neutraubling, triggered our Food & Beverage business field in the late 1990‘s. PreSens supplies sensors for checking the oxygen-tightness of packaging and special systems for deter- mining the penetrability of oxygen in PET bottles at companies such as Nestlé, Heineken or Danisco. Biology & Environmental Our worldwide customer base in biological & environmental re- search has now grown to hundreds of users coming from the University of Alaska in Anchorage to the University of Wellington in New Zealand. For more than two decades we have delivered special sensor systems for various applications such as respi- Life Science Research Surgery Patient Care rometry, or environmental monitoring. Medical Research & Life Sciences Our most recent business field arose from a cooperation with renowned medical technology manufacturers from the medical devices sector. PreSens supplies OEM parts, which are integrated into more complex medical systems. Microsensors, sensor spots, and imaging systems are applied in tissue engineering, micro- fluidics, and many other medical research fields. QC Industry & Technical Applications Robust probes with excellent long-term stability or sensors for contactless measurement find use in technical or industrial applications. Specially designed flow-through connectors for integration in pipes are already applied to monitor the oxygen content in liquids or gases. OEM sensor components can be designed to be integrated in customer systems. www.PreSens.de/biotech-pharma 6 Sensors for the Biotech & Pharma Industries sensors Sensor Solutions Online Monitoring of O2, pH, CO2 and biomass PreSens sensors for Biotech & Pharma help com- } Micro- or even non-invasive measurement panies like Roche and DSM to improve their bio- } Online monitoring of microbial & cell cultures 3 processes. With our non-invasive measurement } From μL to m scale } Bags & single-use bioreactors systems no more time consuming sampling is } Ready-to-use disposables with integrated sensors necessary and they allow simultaneous moni- } Pre-calibrated toring of multiple samples. While our various O2 } Autoclavable sensors & dipping probes sensors suit almost any application, pH sensors } Flow-through cells for perfusion monitoring are optimized for measurements in cultivations, and our CO2 sensors work in selected applications depending on the sterilization method and the process conditions. For O2 and pH even a variety of ready-to-use disposables with integrated and pre-calibrated sensors is available. Customer feedback has influenced our product develop- ment for two decades and we can therefore serve your needs: validated measurement results. Contact us and we find your customized solution! 7 Applications SENSOR SOLUTIONS SENSOR Bioprocess Development: Production: Single-use and Sensors in Small-scale Systems Autoclavable Sensors for Online The online measurement of dissolved oxygen concentration and Culture Monitoring pH in shaken bioreactors paves the way for proper scale up or scale down activities - from bench-top stirred bioreactors to larger or Bags and single-use bioreactors are in the process of revolution- smaller scales. So far, appropriate measurement methods for cul- izing the way biopharmaceuticals are manufactured. Our non-in- ture monitoring in shake flasks were missing. With the application vasive oxygen and pH sensors - integrated in the culture vessels of single-use optical sensors culture conditions can be monitored - are the tools to turn simple disposables into full bioreactors. online without sampling. They enable highly parallelized measure- For steel fermenters our robust probes are the ideal monitoring ments in multiwell plates and flasks. Limitations can be detected device, as they stand autoclaving, SIP, and CIP. The dissolved in time while the online readings give new insights into metabolic oxygen probes are equipped with standardized threads that are activities in shaken cultures. compatible with most bioreactors and port adapters and can be applied for measurement in large-scale cell or microbial cultures. Drug Screening & Strain Quality Control: Micro-invasive Development: Monitoring in & Fast Oxygen Determination Multiwell Culture Vessels Oxygen inside packaging can affect the stability of pharmaceuti- cals. Micro-invasive sensors with steel needles are ideally suited to Microtiter plates and multidishes with integrated oxygen and pH measure the oxygen content e. g. in blisters or pharmaceutical vials. sensors allow high-throughput assays. By monitoring cellular Pierced through the packaging material, these sensors determine respiration, the effect of different concentrations of pharmaceu- the oxygen content inside the headspace as well as in a liquid within tically active substances can be easily determined. In strain de- seconds. It is an easy method to check and ensure the quality of velopment the online dissolved oxygen and pH monitoring gives pharmaceutical products and packaging. valuable information about the metabolic status of cultures at all times, while parallel experiments with different strains or different media composition are conducted. These disposables offer new options, e. g. when developing harvesting strategies and optimizing media. www.PreSens.de/biotech-pharma 8 Sensors for the Biotech & Pharma Industries Sensor Solutions for Biotech & Pharma 8 7 5 2 Contact us and we find your customized solution! 9 OEM Solutions for you PreSens offers customized sensor technology solu- tions. Right from the beginning PreSens can be your partner while finding new approaches: from specifica- SOLUTIONS SENSOR tions to implementation up to production of your tool. Don’t hesitate to ask for your individual solution: [email protected] 1 Sensor Spots for contactless measurement in transparent culture vessels 2 Disposable Shake Flasks (SFS), Multidishes (Oxo- and HydroDishes), Spinner Flasks (SPS), Cell Culture Flasks (CFS), Cell Culture Tubes (iTubes) with integrated sensors 1 3 Oxygen Probes OIM for bioreactors and steel fermenters 4 Flow-through Cells (also disposable 3 versions available) with integrated sensors for perfusion monitoring 4 9 5 Oxygen Microsensors for quality control of pharmaceuticals and their packaging 6 O2 & pH Nice Ports 6 with single-use sensors for customized integration (welding) into bags 7 SFR Shake Flask Reader, SFR vario for O2, pH, biomass and OUR monitoring in shake flasks SDR SensorDish Reader for O2 & pH monitoring in multidishes 8 Robust Oxygen Sensors (sensor spots & probes) that stand autoclaving (up to + 130 °C) 9 Microtiterplates with integrated sensors (O2 or pH) for screening purposes; compatible with most conven- tional fluorescence readers www.PreSens.de/biotech-pharma 10 Sensors for the Biotech
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