Commissioners

Total Page:16

File Type:pdf, Size:1020Kb

Commissioners Chef's inside DELAND/LAKE HELEN DELEON SPRINGS PIERSON Corner His test subjects provide a clue to healthier eating Page B3 @HometownNewsVolusia @Hometownnewsvolusia @HVolusi Vol. 7, No. 9 Your Local News and Information Source • www.HometownNewsVolusia.com Friday, April 13, 2018 Community Commissioners: Loiterers Notes County schedules don’t belong in jail hurricane meeting By Gina McKee me that you don’t have the decriminalized pro- Call us about Advertising in the visions to deal with them.” Volusia County’s Commu- [email protected] Police Chief Jason Umberger agreed, add- Hurricane Guide nity Assistance Division will While most cities are cracking down on loitering, ing it’s not reasonable to arrest someone for host a public meeting with DeLand City Commissioners and city police officers Publishing May 25th ringing a doorbell and running off unless municipalities and the public are working to find middle ground to ensure the SPECIAL EDITIONFor more Information Call 386-322-5900 they refuse to move on and desist. at 10 a.m. Monday, April 16, to punishment fits the crime. “The reason we wanted to amend our par- discuss Community Develop- In a unanimous vote April 2, commissioners voted ticular ordinances to decriminalize the event where pen- ment Block Grant Disaster to decriminalize certain types of loitering, reducing Recovery funds for those folks were coming up and ringing doorbells at dif- alties under misde- the act to a misdemeanor punishable by a citation. affected by Hurricane Mat- ferent times of the evening, so we did amend that to meanor classifications, can be utilized as Rather than hauling a teenager who got a bit out thew. read 10 p.m. to 7 a.m. to mirror our noise ordi- well. of line to jail, some other options needed to be avail- The meeting will be in the nance,” Chief Umberger said. “We do have a state statute called loitering or able, Mayor Robert Apgar said. county council chambers at In those cases, a decriminalized version of the city prowling. It says its unlawful for any person to loiter “Let’s just use some kids hanging out at one of the the Thomas C. Kelly Adminis- statute on the books can serve a dual purpose of or prowl in a place, time or manner not usual for fast-food restaurants as an example, and they’re just tration Center, 123 W. Indiana providing punishment without a criminal charge law-abiding individuals under circumstances that being kids, maybe a little rowdy and all that, but it’s Ave., DeLand. and arrest. justify a normal and reasonable alarm or immediate now 8:30 at night,” Mayor Apgar said. “It seems to County employees will In addition, two state statutes, which carry civil See JAIL, page A2 review the funding require- ments, the unmet housing needs assessment, and pro- posed strategies for a regional application. For more information, call Watching Sunny sculpture Corry Brown at (386) 736- 5955, ext. 12970. butterflies Hospital museum reopens under the trees The DeLand Memorial Hos- By Gina McKee trees.” pital & Veterans Museum [email protected] Now that the designation reopened to the public April 9. of Monarch City USA has The former DeLand Memo- Close on the heels of being been bestowed, city officials rial Hospital, at Bill Dreggors the first city in Florida are encouraging local resi- Park, has served as the main named a Monarch City USA, offices for the city’s Parks and dents to get involved to help DeLand received word of its protect, support and grow Recreation Department after 30th consecutive year as a the facility was repurposed in the monarch butterfly popu- 1993. The building’s second Tree City USA. lation. and third floors have been The honors are a direct According to the Center used as a multi-floor museum result of the city’s focus on, for Biological Diversity, the operated by both the West and commitment to, conser- Monarch butterfly popula- Volusia Historical Society and vation, Mayor Robert Apgar tion was an estimated 682 DeLand Historic Trust Inc. said. million in 1997 compared to The museum was temporar- “The City of DeLand has a 150 million counted in 2016, ily closed last year to refresh long history of supporting and create new exhibits. a nearly 80 percent decline conservation efforts by pre- over a 19-year period. For a tour time, call (386) serving our beautiful oak 626-7280. Because monarchs rely on trees as evidenced by our vanishing milkweed plants Bruce D. Henderson/staff photographer 30-year designation as a and decreasingly available Pam Rocca, left, and Lynn Glynn, right, both of New Smyrna Beach, talk to Friends of library Tree City,” Mayor Apgard nectar-producing plants for said in a news release. “My DeLand artist Karen Tweedie about the sunflower bench by artist Jim Gal- book sale their homes and their food, lucci of Greensboro, N.C., on Friday, April 6, along West Indiana Avenue hope is that decades from Monarach Cities are asked The Friends of the DeLand now, the Monarch butterfly during the Sculpture Walk presented by the Museum of Art-DeLand and the Library’s monthly Book Sale will thrive alongside our See WATCHING, page A2 city. will be from 4:30 p.m. Friday, April 13 and 9:30 to 1 p.m. Sat- urday, April 14 in the Library auditorium at 130 E. Howry Ave., DeLand. Renewals and non-mem- Lots of County bers may purchase member- See NOTES, page A2 fun moves Personal Finance A8 Among $300K PLAN STRONGER the to block Consider health grants insurance Trees with early By Gina McKee retirement By Gina McKee [email protected] [email protected] A weekend of art, music, During a meeting for legis- crafts and more is on tap Sat- lative updates, plans for future Entertainment B1 urday, April 14, for the DeLe- Hometown News file photo programs and county busi- on Springs Community Asso- Children look at artwork created by McInnis Elementary School students ness, the Volusia County DELANDAPALOOZA ciation’s sixth annual Art Council also did some budget Among the Trees festival. at last year’s Art Among the Trees festival presented by the DeLeon Springs spring cleaning. The event, which runs from Community Association. In an effort to close out 9 a.m. until 3 p.m at 5030 N. grant funding through the U.S. 17, DeLeon Springs, will A car show; and a free Kid- $300 each for Best of Show, For those who have string Neighborhood Stabilization have something for everyone zone will include art projects Best Fine Art and Best Fine instruments in disrepair, Program, the County Coun- to enjoy, said Amy Munizzi, and lots of fun activities for Craft with second-place and bring those along, too, she cil approved transferring DSCA president. the kids,” Ms. Munizzi said. third-place ribbons in all cat- added. $300,000 from the NSP1 “In addition to fine art and The activities aren’t the egories.” “We also have a Luthier accounts into the Community crafts on show and for sale, only big draw the day of the Budding musicians also (stringed instrument repair- Development Block Grant Annual music festival there will be live bluegrass, festival, because the art show can bring their instruments man) on site, so people can will take over downtown fund. old-time and rock music on also is a juried event, meaning for free workshops and jam bring in a damaged or an old “The county has expended stage through the day; a deli- sessions with seasoned play- there will be judging and cash instrument and let him bring all of its NSP1 grant funds and cious food court grill and prizes, she added. ers. Index it back to life,” Ms. Munizzi is undertaking administrative goody vendors, McInnis Ele- “ It is a fine arts and music “Musicians will host a free said. “Several people have steps to close out its NSP1 Business A7 Out & About B1 mentary School student art- focused event,” Ms. Munizzi music workshop for anyone been amazed at what he has Classified B6 Police Report A5 work on display; the Crankin said. “As a juried event, there to bring an instrument and been able to do.” grant with the U.S. Depart- Crossword B5 Sports B5 Horoscopes B1 Viewpoint A6 A’s beautifully restored Model are awards in three categories: jam with the pros,” she said. See TREES, page A2 See GRANTS, page A2 3 days 2 night to Savannah, Jekyll Island and Charleston, SC The Cloisters has teamed up with a unique tour group “Diamond Tours” AWESOME The cost is $315.00 per person and includes: Motor coach transportation, 2 nights lodging, OPEN TO OPPORTUNITY! THE PUBLIC! 4 meals (2 breakfast and 2 dinners) Guided Trolley Tour of Savannah, followed by Hop-on/hop-off Privileges, www.thecloistersretirement.org Visit to Historic Charleston SC and a guided tram tour of Jekyll Island. (800)738-1222 | 400 East Howry Ave., DeLand A Retirement Housing Foundation Campus Contact Cheryl Starling 386-822-6900 x208 for Registration details A not-for-profit community - ALF#8340 (The motorcoach leaves from The Cloisters Tuesday October 2nd at 8:00 am and returns Late afternoon Thursday October 4th.) A-2 • DeLand/Lake Helen/DeLeon Springs/Pierson Hometown News Friday, April 13, 2018 persons who may witness violence.” Jail them; or engage in brawling Decriminalizing the city’s Chainsaw artist From page A1 or fighting; or engage in such statute allows for more rea- conduct as to constitute a sonable handling and pro- concern for safety of persons breach of the peace or disor- vides additional options, or property in that vicinity, so derly conduct.” allowing loitering itself to be we could use that (in cases In other cases, however, if a misdemeanor while refus- where criminal charges are the perpetrator refuses to ing to cooperate or resisting not warranted).” the police move on, there are other without violence still carry chief said.
Recommended publications
  • Observation and a Numerical Study of Gravity Waves During Tropical Cyclone Ivan (2008)
    Open Access Atmos. Chem. Phys., 14, 641–658, 2014 Atmospheric www.atmos-chem-phys.net/14/641/2014/ doi:10.5194/acp-14-641-2014 Chemistry © Author(s) 2014. CC Attribution 3.0 License. and Physics Observation and a numerical study of gravity waves during tropical cyclone Ivan (2008) F. Chane Ming1, C. Ibrahim1, C. Barthe1, S. Jolivet2, P. Keckhut3, Y.-A. Liou4, and Y. Kuleshov5,6 1Université de la Réunion, Laboratoire de l’Atmosphère et des Cyclones, UMR8105, CNRS-Météo France-Université, La Réunion, France 2Singapore Delft Water Alliance, National University of Singapore, Singapore, Singapore 3Laboratoire Atmosphères, Milieux, Observations Spatiales, UMR8190, Institut Pierre-Simon Laplace, Université Versailles-Saint Quentin, Guyancourt, France 4Center for Space and Remote Sensing Research, National Central University, Chung-Li 3200, Taiwan 5National Climate Centre, Bureau of Meteorology, Melbourne, Australia 6School of Mathematical and Geospatial Sciences, Royal Melbourne Institute of Technology (RMIT) University, Melbourne, Australia Correspondence to: F. Chane Ming ([email protected]) Received: 3 December 2012 – Published in Atmos. Chem. Phys. Discuss.: 24 April 2013 Revised: 21 November 2013 – Accepted: 2 December 2013 – Published: 22 January 2014 Abstract. Gravity waves (GWs) with horizontal wavelengths ber 1 vortex Rossby wave is suggested as a source of domi- of 32–2000 km are investigated during tropical cyclone (TC) nant inertia GW with horizontal wavelengths of 400–800 km, Ivan (2008) in the southwest Indian Ocean in the upper tropo- while shorter scale modes (100–200 km) located at northeast sphere (UT) and the lower stratosphere (LS) using observa- and southeast of the TC could be attributed to strong local- tional data sets, radiosonde and GPS radio occultation data, ized convection in spiral bands resulting from wave number 2 ECMWF analyses and simulations of the French numerical vortex Rossby waves.
    [Show full text]
  • Dolly the Sheep – the First Cloned Adult Animal
    DOLLY THE SHEEP – THE FIRST CLONED ADULT ANIMAL NEW TECHNOLOGY FOR IMPROVING LIVESTOCK From Squidonius via Wikimedia Commons In 1996, University of Edinburgh scientists celebrated the birth of Dolly the Sheep, the first mammal to be cloned using SCNT cloning is the only technology adult somatic cells. The Edinburgh team’s success followed available that enables generation of 99.8% its improvements to the single cell nuclear transfer (SCNT) genetically identical offspring from selected technique used in the cloning process. individuals of adult animals (including sterilized animals). As such, it is being Dolly became a scientific icon recognised worldwide and exploited as an efficient multiplication tool SCNT technology has spread around the world and has been to support specific breeding strategies of used to clone multiple farm animals. farm animals with exceptionally high genetic The cloning of livestock enables growing large quantities of value. the most productive, disease resistant animals, thus providing more food and other animal products. Sir Ian Wilmut (Inaugural Director of MRC Centre for Regeneration and Professor at CMVM, UoE) and colleagues worked on methods to create genetically improved livestock by manipulation of stem cells using nuclear transfer. Their research optimised interactions between the donor nucleus and the recipient cytoplasm at the time of fusion and during the first cell cycle. Nuclear donor cells were held in mitosis before being released and used as they were expected to be passing through G1 phase. CLONING IN COMMERCE, CONSERVATION OF AGRICULTURE AND PRESERVATION ANIMAL BREEDS OF LIVESTOCK DIVERSITY Cloning has been used to conserve several animal breeds in the recent past.
    [Show full text]
  • Laser Interferometer Space Antenna What Is LISA?
    Volume 11 number 4 2003 FALL Quarter A FLIGHT PROGRAMS AND PROJECTS DIRECTORATE QUARTERLY PUBLICATION A Newsletter Published for Code 400 Employees INSIDE Laser Interferometer Space Antenna THIS What is LISA? ISSUE: How did the Universe begin? Does time have a beginning and an end? Does What is LISA? Page 1 space have edges? These are the questions we've struggled to answer for cen- Solar Dynamic Observatory Page 1 turies. Science and technology have now reached the point where answers to these questions are finally within our grasp. The Laser Interferometer Space Message From The Director Of Page 2 Antenna (LISA) may supply some of these answers as the mission studies Peer Award Ceremony & Picnic Page 2 the mergers of supermassive black holes, tests Einstein's Theory of General Tintypes Page 3 Relativity, probes the early Universe, and searches for gravitational waves—— its primary objective. Feedback Page 3 As the first dedicated space-based gravitational wave observatory, LISA will Technology Corner Page 6 detect waves generated by binaries within our Galaxy (the Milky Way) and Quotes of the Quarter Page 7 by massive black holes in distant galaxies. LISA will use an advanced system of laser interferometry for directly detecting and measuring them. This OBPR Free-Flyer Page 10 Best of the Best Page 12 (LISA Continued on page 4) The English Language Page 13 Peer Awards Page 14 Living with a Star Program – NASA Honor Awards Page 16 Solar Dynamics Observatory TCP Social News Page 19 Developing an understanding of the Sun took on a renewed sense of ur- Cultural Tidbits Page 19 gency during late October/early November.
    [Show full text]
  • Guide to Biotechnology 2008
    guide to biotechnology 2008 research & development health bioethics innovate industrial & environmental food & agriculture biodefense Biotechnology Industry Organization 1201 Maryland Avenue, SW imagine Suite 900 Washington, DC 20024 intellectual property 202.962.9200 (phone) 202.488.6301 (fax) bio.org inform bio.org The Guide to Biotechnology is compiled by the Biotechnology Industry Organization (BIO) Editors Roxanna Guilford-Blake Debbie Strickland Contributors BIO Staff table of Contents Biotechnology: A Collection of Technologies 1 Regenerative Medicine ................................................. 36 What Is Biotechnology? .................................................. 1 Vaccines ....................................................................... 37 Cells and Biological Molecules ........................................ 1 Plant-Made Pharmaceuticals ........................................ 37 Therapeutic Development Overview .............................. 38 Biotechnology Industry Facts 2 Market Capitalization, 1994–2006 .................................. 3 Agricultural Production Applications 41 U.S. Biotech Industry Statistics: 1995–2006 ................... 3 Crop Biotechnology ...................................................... 41 U.S. Public Companies by Region, 2006 ........................ 4 Forest Biotechnology .................................................... 44 Total Financing, 1998–2007 (in billions of U.S. dollars) .... 4 Animal Biotechnology ................................................... 45 Biotech
    [Show full text]
  • Evolution of Multiscale Vortices in the Development of Hurricane Dolly (2008)
    JANUARY 2011 F A N G A N D Z H A N G 103 Evolution of Multiscale Vortices in the Development of Hurricane Dolly (2008) JUAN FANG Key Laboratory of Mesoscale Severe Weather (MOE), Department of Atmospheric Sciences, Nanjing University, Nanjing, China FUQING ZHANG Department of Meteorology, The Pennsylvania State University, University Park, Pennsylvania (Manuscript received 6 April 2010, in final form 6 July 2010) ABSTRACT As a follow-up to a previously published article on the initial development and genesis of Hurricane Dolly (2008), this study further examines the evolution of, and interactions among, multiscale vortices ranging from the system-scale main vortex (L . 150 km) to the intermediate-scale cloud clusters (50 km , L , 150 km) and individual vorticity-rich convective cells (L , 50 km). It is found that there are apparent self-similarities among these vortices at different scales, each of which may undergo several cycles of alternating accumulation and release of convective available potential energy. Enhanced surface fluxes below individual cyclonic vortices at each scale contribute to the sustainment and reinvigoration of moist convection that in turn contributes to the maintenance and upscale growth of these vortices. Spectral analysis of horizontal divergence and relative vorticity further suggests that the cloud-cluster-scale and system-scale vortices are predominantly balanced while the individual convective vortices are largely unbalanced. The vorticity and energy produced by these individual vorticity-rich convective cells first saturate at convective scales that are subsequently transferred to larger scales. The sum of the diabatic heating released from these convective cells may be regarded as a persistent forcing on the quasi-balanced system-scale vortex.
    [Show full text]
  • Development of Transgenic Livestock with Reduced Myostatin
    DEVELOPMENT OF TRANSGENIC LIVESTOCK WITH REDUCED MYOSTATIN EXPRESSION USING RNA INTERFERENCE A Dissertation by KIMBERLY JEAN TESSANNE Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY December 2009 Major Subject: Veterinary Physiology DEVELOPMENT OF TRANSGENIC LIVESTOCK WITH REDUCED MYOSTATIN EXPRESSION USING RNA INTERFERENCE A Dissertation by KIMBERLY JEAN TESSANNE Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Approved by: Chair of Committee, Mark Westhusin Committee Members, Charles Long Thomas Spencer Nancy Ing Head of Department, Glen Laine December 2009 Major Subject: Veterinary Physiology iii ABSTRACT Development of Transgenic Livestock with Reduced Myostatin Expression Using RNA Interference. (December 2009) Kimberly Jean Tessanne, B.S., The Ohio State University; M.S., University of Wisconsin-Madison Chair of Advisory Committee: Dr. Mark Westhusin RNA interference (RNAi) is a means of regulating gene expression by targeting mRNA in a sequence-specific manner for degradation or translational inhibition. Short hairpin RNAs (shRNAs) and short interfering RNAs (siRNAs) have been extensively employed for manipulating gene expression in a wide range of species. The goal for this research was to produce transgenic livestock in which myostatin, a negative regulator of muscle growth, has been targeted for silencing by RNAi. This would demonstrate the utility of RNAi for reducing gene expression in large animal species. To successfully target the myostatin gene for reduction, siRNAs were designed to target the both the bovine and caprine myostatin mRNA sequence.
    [Show full text]
  • The Rotary Club of Richmond
    TABLE OF CONTENTS RI President Kalyan Banerjee Bio ..................................4 INTERNATIONAL SERVICE RI President Kalyan Banerjee Letter ..............................5 2008 Refilwe Project .....................................................50 Letter from PM Stephen Harper .....................................6 Disaster Relief ...............................................................51 Letter from MP Alice Wong ...........................................7 2007 Joint Wheelchair Project .....................................52 Letter from Premier Christy Clark ..................................8 Aid for Children in Trinidad .........................................52 Letter from MLA Linda Reid ..........................................9 Korle-Bu Neuroscience .................................................53 Letter from Mayor Malcolm Brodie ..............................10 2009 Shoes for Sri Lanka ..............................................53 Letter from District Governor Hans Doge ...................11 The Rotary Foundation.................................................54 Rotary Club President Ken Whitney Bio ......................12 PolioPlus ........................................................................54 About Rotary & Paul P. Harris ......................................13 Paul Harris Fellows ........................................................55 Guiding Principles .........................................................14 Ambulance Projects ......................................................56
    [Show full text]
  • Cloning After Dolly
    Cloning after Dolly Professor Derek Burke 'Cloning' is a loose term used to describe everything from a new super race of identical near- robots (remember 'The Boys from Brazil'?) to propagating your roses. It actually means a twig, so it starts from the horticultural end, but is used much more widely. Dolly was the first cloned mammal and must be the most famous sheep in the world. Two things made her famous: first that she is an artificially induced clone, genetically identical to a previously existing sheep, for Dolly can be thought of as a vertical twin with identical genes, in this case to the parent. Second is the way in which it was done. The scientists took the nucleus from a cell from the udder of an adult sheep and implanted it in an egg cell whose original nucleus had been removed. Very surprisingly, the adult cell DNA was able to give rise to a complete individual, not just more udder cells. This was surprising because, although at the earliest stage of development the DNA is still pluripotent, capable of generating the growth of the whole animal, later in development, specialization sets in, and cells become differentiated into heart cells, liver cells etc. The whole of the genetic material is still there, but most of it is switched off and only the genes relevant to that particular cell are active. It had been thought that this process of differentiation in mammals was irreversible, so that an udder cell could only give rise to more udder cells. But we now know that differentiation can be reversed.
    [Show full text]
  • Cessation of Anadromy in a Long-Lived Fish
    Ecology, 96(7), 2015, pp. 1899–1910 Ó 2015 by the Ecological Society of America Beyond dichotomous life histories in partially migrating populations: cessation of anadromy in a long-lived fish 1,3 2 1 MORGAN H. BOND, JESSICA A. MILLER, AND THOMAS P. QUINN 1School of Aquatic and Fishery Sciences, University of Washington, Box 355020, Seattle, Washington 98195 USA 2Coastal Oregon Marine Experiment Station, Hatfield Marine Science Center, Department of Fisheries and Wildlife, Oregon State University, 2030 SE Marine Science Drive, Newport, Oregon 97365 USA Abstract. Across animal taxa, migration allows individuals to exploit habitats and resources that predictably vary seasonally in suitability. Theory predicts that the ‘‘decision’’ to migrate or not is shaped by the relative fitness costs and benefits of exhibiting a given life history. Adoption of a migratory strategy is widely thought to reflect a dichotomous outcome; individuals are either resident or migratory, and continue to exhibit this life history until death. In fishes, anadromy and freshwater residency represents a well-studied life history dichotomy. Resident individuals may adopt a migratory life history later in life, but migratory individuals are not known to abandon this pattern. Here, we investigated the fitness benefits, as measured by body size, of residency and anadromy in a salmonid fish, Dolly Varden, Salvelinus malma, in Alaska, and reveal a novel life history: cessation of migration by older, larger individuals. Otolith microchemical analysis of Dolly Varden showed that while most fish migrated to sea at least once in their lives, lifelong resident fish exist in streams with close proximity to the ocean.
    [Show full text]
  • Genetic Cloning and Testing
    Genetic Cloning and Testing BACKGROUND Genetic Cloning or years, humans successfully have used myriad genetic techniques—from GLOSSARY Fselective breeding to genetic manipulation—to create plants and animals Chromosome with desirable characteristics. Corn has been genetically engineered to improve Units in the nucleus of a cell, resistance to fungus and viral infections, rot, and drought, and calves have been composed of DNA, that contain genes; normal human somatic cells genetically altered to accelerate the growth process, to cite two examples. contain 46 chromosomes (23 pair). Clone Genetic cloning is a comparatively new process that uses the DNA contained in Duplicate. cells to “clone,” or duplicate, strands of DNA, cells, or organisms to achieve DNA Deoxyribonucleic acid; the material exact copies of the original. These genetic clones are analogous to identical inside the nucleus of a cell that human twins—two genetically identical individuals whose development can be carries genetic information. traced to the division of a single embryo. For some time, scientists have known Embryo The prefetal product of conception that multiple animals could be created in the same way that human twins are from implantation through the 12th created: by dividing a single embryo into multiple embryos shortly after fertiliza- week of development. An embryo conceived through sexual tion. This creates offspring that are genetically identical to each other and have reproduction receives half its genetic received their genetic information from each sexual parent (half from the male, information from each sexual parent—that is, half comes from half from the female). the sperm and half from the egg.
    [Show full text]
  • Cloning a Plasmid
    Dolly, herself http://oracleformsinfo.files.wordpress.com/2012/03/clone.jpg Dolly the sheep, who became famous as the first mammal to be cloned from an adult cell, has died. The news was confirmed on Friday by the Roslin Institute, the Scottish research centre which created her. A decision was taken to "euthanize" six-year-old Dolly after a veterinary examination showed that she had a progressive lung disease, the institute said in a statement. Dolly became the first mammal clone when she was born on 5 July 1996. She was revealed to the public the following year. http://martinfrost.ws/htmlfiles/roslin_dolly.html What is a clone? A clone is a • Pieces of DNA group of • Plasmids genetically • Bacteria identical … ummm….. • Eukaryotic cells in vivo • Eukaryotic cells Thingies? • Organisms • Other examples? http://media.moddb.com/images/downloads/1/25/24418/Star_wars_the_clone_wars.jpg To DNA (& etc) http://anglelakesc.blogspot.com/2011/10/road-construction-detours.html Cloning a plasmid http://wiki.biomine.skelleftea.se/wiki/images/9/93/Cloning_with_the_help_of_LacZ_.png Do clones occur in nature? • Identical twins • Lymphocytes • An embryo … http://cadencemag.com/wp-content/uploads/2012/03/twins.gif YOU are a clone of your original fertilized egg! Are cloned cells identical? -- genetically? -- functionally? How? Why? Why not? Dolly & her baby – so Dolly was fertile Fertility is a common measure of whether a genome is really complete http://news.bbc.co.uk/2/hi/science/nature/2764039.stm First Cloned Mouse Dies Of Old Age: Technique Believed More Reliable Than That Used For Dolly The Sheephttp://www.cbsnews.com/stories/2000/05/10/tech/main193799.shtml • Why clone? • Clone what ? • Humans – Whole? • Financial benefit – Parts? • Convenience • Animals • Uniformity – Agricultural • Personal emotion – Environmental restoration • Medical intervention – Fun – e.g.
    [Show full text]
  • BSCI410-Liu/SP09 Midterm Exam #2/Apr. 14 Your Name
    BSCI410-Liu/SP09 Midterm Exam #2/Apr. 14 Your Name: 1. (28 points total) Scientists are trying to unlock the molecular basis of a mysterious hereditary disease named DEAN SYMDROM (DS). ds mutant mice show a lack of memory and ds/ds mutant mice, when crossed with wild type (+/+) mice, produces progeny that also show memory loss. (a) (4 points) Is the ds mutation recessive or dominant? dominant (b) (4 points) Using molecular markers such as SNPs, scientists have mapped the putative DS gene to a small chromosomal region. This region contains three genes: X, Y, and Z. Design an experiment to test the expression of X, Y, and Z genes. Which tissue do you expect the candidate DS gene to be expressed in? Northern blot, RT-PCR, in situ hybridization to see which gene is expressed in brain tissue (c) (8 points) Through the above experiments, scientists identified gene Y that is expressed in the expected tissue. List two additional experiments that will definitely prove that gene Y is the DS gene. 1) sequence mutant mice DNA and compare with WT. If one detects a mutation in Y in the mutant mice, it would prove it. 2) Transgenic study - Introduce the mutant gene into the WT mouse to see if it causes memory loss (cannot introduce WT into mutant mouse to rescue because the mutation is dominant) I also took other reasonable answers. NOTE: Testing RNA expression will not definitely prove that gene Y is the DS gene. (d) (8 points) After identifying the mouse DS gene, you now have the DNA sequence of the mouse DS gene.
    [Show full text]