Mouse Taar3 Knockout Project (CRISPR/Cas9)
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Identification of a Subset of Trace Amine-Associated Receptors and Ligands As Potential Modulators of Insulin Secretion
Journal Pre-proofs Identification of a subset of trace amine-associated receptors and ligands as po- tential modulators of insulin secretion Michael J. Cripps, Marta Bagnati, Tania A. Jones, Babatunji W. Ogunkolade, Sophie R. Sayers, Paul W. Caton, Katie Hanna, Merell Billacura, Kathryn Fair, Carl Nelson, Robert Lowe, Graham A. Hitman, Mark D. Berry, Mark D. Turner PII: S0006-2952(19)30384-3 DOI: https://doi.org/10.1016/j.bcp.2019.113685 Reference: BCP 113685 To appear in: Biochemical Pharmacology Received Date: 22 August 2019 Accepted Date: 24 October 2019 Please cite this article as: M.J. Cripps, M. Bagnati, T.A. Jones, B.W. Ogunkolade, S.R. Sayers, P.W. Caton, K. Hanna, M. Billacura, K. Fair, C. Nelson, R. Lowe, G.A. Hitman, M.D. Berry, M.D. Turner, Identification of a subset of trace amine-associated receptors and ligands as potential modulators of insulin secretion, Biochemical Pharmacology (2019), doi: https://doi.org/10.1016/j.bcp.2019.113685 This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. © 2019 Elsevier Inc. All rights reserved. -
G Protein-Coupled Receptors
S.P.H. Alexander et al. The Concise Guide to PHARMACOLOGY 2015/16: G protein-coupled receptors. British Journal of Pharmacology (2015) 172, 5744–5869 THE CONCISE GUIDE TO PHARMACOLOGY 2015/16: G protein-coupled receptors Stephen PH Alexander1, Anthony P Davenport2, Eamonn Kelly3, Neil Marrion3, John A Peters4, Helen E Benson5, Elena Faccenda5, Adam J Pawson5, Joanna L Sharman5, Christopher Southan5, Jamie A Davies5 and CGTP Collaborators 1School of Biomedical Sciences, University of Nottingham Medical School, Nottingham, NG7 2UH, UK, 2Clinical Pharmacology Unit, University of Cambridge, Cambridge, CB2 0QQ, UK, 3School of Physiology and Pharmacology, University of Bristol, Bristol, BS8 1TD, UK, 4Neuroscience Division, Medical Education Institute, Ninewells Hospital and Medical School, University of Dundee, Dundee, DD1 9SY, UK, 5Centre for Integrative Physiology, University of Edinburgh, Edinburgh, EH8 9XD, UK Abstract The Concise Guide to PHARMACOLOGY 2015/16 provides concise overviews of the key properties of over 1750 human drug targets with their pharmacology, plus links to an open access knowledgebase of drug targets and their ligands (www.guidetopharmacology.org), which provides more detailed views of target and ligand properties. The full contents can be found at http://onlinelibrary.wiley.com/doi/ 10.1111/bph.13348/full. G protein-coupled receptors are one of the eight major pharmacological targets into which the Guide is divided, with the others being: ligand-gated ion channels, voltage-gated ion channels, other ion channels, nuclear hormone receptors, catalytic receptors, enzymes and transporters. These are presented with nomenclature guidance and summary information on the best available pharmacological tools, alongside key references and suggestions for further reading. -
MHC-Dependent Mate Choice Is Linked to a Trace-Amine-Associated Receptor Gene in a Mammal Received: 25 September 2015 Pablo S
www.nature.com/scientificreports OPEN MHC-dependent mate choice is linked to a trace-amine-associated receptor gene in a mammal Received: 25 September 2015 Pablo S. C. Santos1,2, Alexandre Courtiol1,3, Andrew J. Heidel4,†, Oliver P. Höner1, Accepted: 11 November 2016 Ilja Heckmann1, Martina Nagy5,‡, Frieder Mayer5, Matthias Platzer4, Christian C. Voigt1 Published: 12 December 2016 & Simone Sommer1,2 Major histocompatibility complex (MHC) genes play a pivotal role in vertebrate self/nonself recognition, parasite resistance and life history decisions. In evolutionary terms, the MHC’s exceptional diversity is likely maintained by sexual and pathogen-driven selection. Even though MHC-dependent mating preferences have been confirmed for many species, the sensory and genetic mechanisms underlying mate recognition remain cryptic. Since olfaction is crucial for social communication in vertebrates, variation in chemosensory receptor genes could explain MHC-dependent mating patterns. Here, we investigated whether female mate choice is based on MHC alleles and linked to variation in chemosensory trace amine-associated receptors (TAARs) in the greater sac-winged bat (Saccopteryx bilineata). We sequenced several MHC and TAAR genes and related their variation to mating and paternity data. We found strong evidence for MHC class I-dependent female choice for genetically diverse and dissimilar males. We also detected a significant interaction between mate choice and the female TAAR3 genotype, with TAAR3-heterozygous females being more likely to choose MHC-diverse males. These results suggest that TAARs and olfactory cues may be key mediators in mammalian MHC-dependent mate choice. Our study may help identify the ligands involved in the chemical communication between potential mates. -
![[ I]-3-Iodothyronamine in Mouse in Vivo: Relationship with Trace](https://docslib.b-cdn.net/cover/3443/i-3-iodothyronamine-in-mouse-in-vivo-relationship-with-trace-1983443.webp)
[ I]-3-Iodothyronamine in Mouse in Vivo: Relationship with Trace
223 Distribution of exogenous [125I]-3-iodothyronamine in mouse in vivo: relationship with trace amine-associated receptors Grazia Chiellini1, Paola Erba2, Vittoria Carnicelli1, Chiara Manfredi2, Sabina Frascarelli1, Sandra Ghelardoni1, Giuliano Mariani2 and Riccardo Zucchi1 1Dipartimento di Scienze dell’Uomo e dell’Ambiente and 2Dipartimento di Oncologia, University of Pisa, Via Roma 55, 56126 Pisa, Italy (Correspondence should be addressed to G Chiellini; Email: [email protected]) Abstract 3-Iodothyronamine (T1AM) is a novel chemical messenger, intestine, liver, and kidney. Tissue radioactivity decreased structurally related to thyroid hormone, able to interact with exponentially over time, consistent with biliary and urinary G protein-coupled receptors known as trace amine-associated excretion, and after 24 h, 75% of the residual radioactivity was receptors (TAARs). Little is known about the physiological detected in liver, muscle, and adipose tissue. TAARs were role of T1AM. In this prospective, we synthesized expressed only at trace amounts in most of the tissues, the 125 [ I]-T1AM and explored its distribution in mouse after exceptions being TAAR1 in stomach and testis and TAAR8 injecting in the tail vein at a physiological concentration in intestine, spleen, and testis. Thus, while T1AM has a (0.3 nM). The expression of the nine TAAR subtypes was systemic distribution, TAARs are only expressed in certain 125 evaluated by quantitative real-time PCR. [ I]-T1AM was tissues suggesting that other high-affinity molecular targets taken up by each organ. A significant increase in tissue vs besides TAARs exist. blood concentration occurred in gallbladder, stomach, Journal of Endocrinology (2012) 213, 223–230 Introduction the physiological role of T1AM is still uncertain, this compound has recently been detected also in human blood The term thyroid hormone (TH) refers to 3,5,30,50- (Saba et al. -
Pattern of TAAR5 Expression in the Human Brain Based on Transcriptome Datasets Analysis
International Journal of Molecular Sciences Article Pattern of TAAR5 Expression in the Human Brain Based on Transcriptome Datasets Analysis Anastasia N. Vaganova 1 , Ramilya Z. Murtazina 1 , Taisiia S. Shemyakova 1 , Andrey D. Prjibelski 1 , Nataliia V. Katolikova 1 and Raul R. Gainetdinov 1,2,* 1 Institute of Translational Biomedicine, St. Petersburg State University, Universitetskaya nab. 7/9, 199034 St. Petersburg, Russia; [email protected] (A.N.V.); [email protected] (R.Z.M.); [email protected] (T.S.S.); [email protected] (A.D.P.); [email protected] (N.V.K.) 2 St. Petersburg University Hospital, St. Petersburg State University, Universitetskaya nab. 7/9, 199034 St. Petersburg, Russia * Correspondence: [email protected] Abstract: Trace amine-associated receptors (TAAR) recognize organic compounds, including primary, secondary, and tertiary amines. The TAAR5 receptor is known to be involved in the olfactory sensing of innate socially relevant odors encoded by volatile amines. However, emerging data point to the involvement of TAAR5 in brain functions, particularly in the emotional behaviors mediated by the limbic system which suggests its potential contribution to the pathogenesis of neuropsychiatric diseases. TAAR5 expression was explored in datasets available in the Gene Expression Omnibus, Allen Brain Atlas, and Human Protein Atlas databases. Transcriptomic data demonstrate ubiquitous low TAAR5 expression in the cortical and limbic brain areas, the amygdala and the hippocampus, Citation: Vaganova, A.N.; the nucleus accumbens, the thalamus, the hypothalamus, the basal ganglia, the cerebellum, the Murtazina, R.Z.; Shemyakova, T.S.; substantia nigra, and the white matter. Altered TAAR5 expression is identified in Down syndrome, Prjibelski, A.D.; Katolikova, N.V.; Gainetdinov, R.R. -
The Origin and Molecular Evolution of Two Multigene Families: G-Protein Coupled Receptors and Glycoside Hydrolase Families
University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Dissertations and Theses in Biological Sciences Biological Sciences, School of Fall 9-25-2013 THE ORIGIN AND MOLECULAR EVOLUTION OF TWO MULTIGENE FAMILIES: G-PROTEIN COUPLED RECEPTORS AND GLYCOSIDE HYDROLASE FAMILIES Seong-il Eyun University of Nebraska - Lincoln, [email protected] Follow this and additional works at: https://digitalcommons.unl.edu/bioscidiss Part of the Bioinformatics Commons, and the Evolution Commons Eyun, Seong-il, "THE ORIGIN AND MOLECULAR EVOLUTION OF TWO MULTIGENE FAMILIES: G-PROTEIN COUPLED RECEPTORS AND GLYCOSIDE HYDROLASE FAMILIES" (2013). Dissertations and Theses in Biological Sciences. 57. https://digitalcommons.unl.edu/bioscidiss/57 This Article is brought to you for free and open access by the Biological Sciences, School of at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Dissertations and Theses in Biological Sciences by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. THE ORIGIN AND MOLECULAR EVOLUTION OF TWO MULTIGENE FAMILIES: G- PROTEIN COUPLED RECEPTORS AND GLYCOSIDE HYDROLASE FAMILIES by Seong-il Eyun A DISSERTATION Presented to the Faculty of The Graduate College at the University of Nebraska In Partial Fulfillment of Requirements For the Degree of Doctor of Philosophy Major: Biological Sciences Under the Supervision of Professor Etsuko Moriyama Lincoln, Nebraska August, 2013 THE ORIGIN AND MOLECULAR EVOLUTION OF TWO MULTIGENE FAMILIES: G- PROTEIN COUPLED RECEPTORS AND GLYCOSIDE HYDROLASE FAMILIES Seong-il Eyun, Ph.D. University of Nebraska, 2013 Advisor: Etsuko Moriyama Multigene family is a group of genes that arose from a common ancestor by gene duplication. Gene duplications are a major driving force of new function acquisition. -
Dimethyltryptamine: Endogenous Role and Therapeutic Potential
2017/2018 Alexandra Verónica Salgado Leite Rodrigues Dimethyltryptamine: endogenous role and therapeutic potential Março, 2018 Alexandra Verónica Salgado Leite Rodrigues Dimethyltryptamine: endogenous role and therapeutic potential Mestrado Integrado em Medicina Área: Farmacologia, Psiquiatria Tipologia: Monografia Trabalho efetuado sob a Orientação de: Doutora Maria Augusta Vieira Coelho Trabalho organizado de acordo com as normas da revista: Journal of Affective Disorders Março, 2018 ` Review Article Corresponding Authors: Alexandra VSL Rodrigues Address: Department of Biomedicine-Pharmacology and Therapeutics Unit FACULDADE DE MEDICINA DA UNIVERSIDADE DO PORTO Al. Prof. Hernâni Monteiro, 4200 - 319 Porto, PORTUGAL e-mail: [email protected] Dimethyltryptamine: endogenous role and therapeutic potential Alexandra VSL Rodrigues 1, Maria A Viera-Coelho 1,2 Faculdade de Medicina da Universidade do Porto, Portugal 1 Department of Biomedicine-Pharmacology and Therapeutics unit, Faculty of Medicine, University of Porto, Porto, Portugal. 2 Psychiatry and Mental Health Clinic, Hospital de São João, Porto, Portugal. Abstract N, N-dimethyltryptamine (DMT) is an indole alkaloid produced by a number of plants and animals, including humans. Its psychoactive effects were first described in 1956 by Stephen Szára, but have been exploited for centuries by South American indigenous populations throught the use of ayahuasca, an infusion made with a mixture of plants rich in the psychadelic DMT. In the present review, we assess the state of the art regarding a putative role for endogenous DMT and potential future clinical applications. We gathered papers published until 20 March 2018 and included in the PubMed database using the words: N,N- dimethyltryptamine and ayahuasca. While the role of endogenous DMT remains unclear, ayahuasca has promising results in anxiety, depression and substance dependence. -
RT² Profiler PCR Array (384-Well Format) Mouse G Protein Coupled Receptors 384HT
RT² Profiler PCR Array (384-Well Format) Mouse G Protein Coupled Receptors 384HT Cat. no. 330231 PAMM-3009ZE For pathway expression analysis Format For use with the following real-time cyclers RT² Profiler PCR Array, Applied Biosystems® models 7900HT (384-well block), Format E ViiA™ 7 (384-well block); Bio-Rad CFX384™ RT² Profiler PCR Array, Roche® LightCycler® 480 (384-well block) Format G Description The Mouse G Protein Coupled Receptors 384HT RT² Profiler™ PCR Array profiles the expression of a comprehensive panel of 370 genes encoding the most important G Protein Coupled Receptors (GPCR). GPCR regulate a number of normal biological processes and play roles in the pathophysiology of many diseases upon dysregulation of their downstream signal transduction activities. As a result, they represent 30 percent of the targets for all current drug development. Developing drug screening assays requires a survey of which GPCR the chosen cell-based model system expresses, to determine not only the expression of the target GPCR, but also related GPCR to assess off-target side effects. Expression of other unrelated GPCR (even orphan receptors whose ligand are unknown) may also correlate with off-target side effects. The ligands that bind and activate the receptors on this array include neurotransmitters and neuropeptides, hormones, chemokines and cytokines, lipid signaling molecules, light-sensitive compounds, and odorants and pheromones. The normal biological processes regulated by GPCR include, but are not limited to, behavioral and mood regulation (serotonin, dopamine, GABA, glutamate, and other neurotransmitter receptors), autonomic (sympathetic and parasympathetic) nervous system transmission (blood pressure, heart rate, and digestive processes via hormone receptors), inflammation and immune system regulation (chemokine receptors, histamine receptors), vision (opsins like rhodopsin), and smell (olfactory receptors for odorants and vomeronasal receptors for pheromones). -
Single Olfactory Receptors Set Odor Detection Thresholds
ARTICLE DOI: 10.1038/s41467-018-05129-0 OPEN Single olfactory receptors set odor detection thresholds Adam Dewan1, Annika Cichy1, Jingji Zhang1, Kayla Miguel1, Paul Feinstein2, Dmitry Rinberg3 & Thomas Bozza 1 In many species, survival depends on olfaction, yet the mechanisms that underlie olfactory sensitivity are not well understood. Here we examine how a conserved subset of olfactory receptors, the trace amine-associated receptors (TAARs), determine odor detection fi 1234567890():,; thresholds of mice to amines. We nd that deleting all TAARs, or even single TAARs, results in significant odor detection deficits. This finding is not limited to TAARs, as the deletion of a canonical odorant receptor reduced behavioral sensitivity to its preferred ligand. Remarkably, behavioral threshold is set solely by the most sensitive receptor, with no contribution from other highly sensitive receptors. In addition, increasing the number of sensory neurons (and glomeruli) expressing a threshold-determining TAAR does not improve detection, indicating that sensitivity is not limited by the typical complement of sensory neurons. Our findings demonstrate that olfactory thresholds are set by the single highest affinity receptor and suggest that TAARs are evolutionarily conserved because they determine the sensitivity to a class of biologically relevant chemicals. 1 Department of Neurobiology, Northwestern University, Evanston, IL 60208, USA. 2 Department of Biological Sciences, Hunter College, CUNY, New York, NY 10065, USA. 3 NYU Neuroscience Institute, New York University Langone Medical Center, New York, NY 10016, USA. Correspondence and requests for materials should be addressed to T.B. (email: [email protected]) NATURE COMMUNICATIONS | (2018) 9:2887 | DOI: 10.1038/s41467-018-05129-0 | www.nature.com/naturecommunications 1 ARTICLE NATURE COMMUNICATIONS | DOI: 10.1038/s41467-018-05129-0 o artificial chemical detector can match the simultaneous (Fig. -
Methamphetamine Induces Trace Amine‐Associated Receptor 1 (TAAR1) Expression in Human T Lymphocytes: Role in Immunomodulation
Article Methamphetamine induces trace amine- associated receptor 1 (TAAR1) expression in human T lymphocytes: role in immunomodulation Uma Sriram, Jonathan M. Cenna, Bijayesh Haldar, Nicole C. Fernandes, Roshanak Razmpour, Shongshan Fan, Servio H. Ramirez, and Raghava Potula1 Department of Pathology and Laboratory Medicine, Temple University School of Medicine, Philadelphia, Pennsylvania, USA RECEIVED AUGUST 13, 2014; REVISED JULY 27, 2015; ACCEPTED AUGUST 5, 2015. DOI: 10.1189/jlb.4A0814-395RR ABSTRACT Introduction The novel transmembrane G protein-coupled receptor, Among the various substances of abuse, METH is gaining trace amine-associated receptor 1 (TAAR1), represents increasing popularity among drug-abusing populations world- a potential, direct target for drugs of abuse and mono- wide for its addictive psychostimulant effects [1, 2]. Recreational aminergic compounds, including amphetamines. For the METH use is one of the fastest growing substance-abuse first time, our studies have illustrated that there is an problems in the United States [3, 4]. METH use is associated with induction of TAAR1 mRNA expression in resting high-risk sexual behavior and high rates of HIV acquisition [5]. T lymphocytes in response to methamphetamine. Meth- amphetamine treatment for 6 h significantly increased Animal and human studies have demonstrated that METH – TAAR1 mRNA expression (P , 0.001) and protein ex- suppresses innate and adaptive immunity [6 8]. More insights pression (P , 0.01) at 24 h. With the use of TAAR1 gene into the mechanism of action of METH have been revealed in silencing, we demonstrate that methamphetamine- the last decade with the discovery of the receptor(s) for METH induced cAMP, a classic response to methamphetamine that belong to specific families of GPCRs [9, 10]. -
Supporting Information
Supporting Information Hussain et al. 10.1073/pnas.0803229106 ORs Zebrafish Taars 862 Neoteleost Taars AmR Shark Taars 769 944 Frog Taars 999 995 Pm Mammalian Taars AmR Chicken Taars MP 66%-100% MP 33%-66% MP 0%-33% 503 Taar20 Supported by 3 methods: NJ, MP and ML 1000 Supported by 2 methods: NJ and ML 742 919 Taar19 545 1000 997 499 Taar18 772 954 997 Taar17 589 1000 1000 1000 1000 Taar16 1000 Taar15 1000 Taar14 985 Class III 1000 Taar28 468 1000 Taar26 783 984 381 1000 266 Taar25 524 387 456 Taar24 999 346 869 Taar23 354 522 1000 1000 855 999 Taar22 900 999 990 999 Taar9 543 1000 926 Taar8 844 998 841 Taar6 918 426 1000 954 Taar7 1000 344 Class II 1000 Taar5 1000 Taar13 238 1000 347 Taar12 773 956 Taar4 400 1000 878 1000 Taar3 1000 849 Taar2 Taar21 1000 Class I 401 1000 Taar11 813 367 Taar27 429 748 Taar10 1000 443 0.05 615 Taar1 Fig. S1. Phylogenetic tree of the taar genes. The cladogram shown here corresponds to the unrooted tree in Fig. 1. The tree is constructed by using the neighbor-joining algorithm; bootstrap support at major nodes is indicated by numbers (1,000 cycles). All subfamilies are supported by all 3 tree algorithms used (neighbor joining; maximum parsimony, 100 bootstraps; maximum likelihood), except subfamilies 23 and 24 (supported by 2 methods). Red lines represent zebrafish taar genes; orange lines, neoteleost taar genes; dark blue, cartilaginous fish taar genes; green, amphibian taar genes; light-blue, mammalian taar genes; and black represents the outgroup (OR, odorant receptors; AmR, aminergic receptors; PmAmR, Petromyzon marinus (sea lamprey) aminergic receptors). -
Neural and Hormonal Basis of Opposite-Sex Preference by Chemosensory Signals
International Journal of Molecular Sciences Review Neural and Hormonal Basis of Opposite-Sex Preference by Chemosensory Signals Yasuhiko Kondo * and Himeka Hayashi Department of Animal Sciences, Faculty of Life and Environmental Science, Teikyo University of Science, Uenohara 409-0193, Yamanashi, Japan; [email protected] * Correspondence: [email protected]; Tel.: +81-554-63-6828 Abstract: In mammalian reproduction, sexually active males seek female conspecifics, while estrous females try to approach males. This sex-specific response tendency is called sexual preference. In small rodents, sexual preference cues are mainly chemosensory signals, including pheromones. In this article, we review the physiological mechanisms involved in sexual preference for opposite-sex chemosensory signals in well-studied laboratory rodents, mice, rats, and hamsters of both sexes, especially an overview of peripheral sensory receptors, and hormonal and central regulation. In the hormonal regulation section, we discuss potential rodent brain bisexuality, as it includes neural substrates controlling both masculine and feminine sexual preferences, i.e., masculine preference for female odors and the opposite. In the central regulation section, we show the substantial circuit regulating sexual preference and also the influence of sexual experience that innate attractants activate in the brain reward system to establish the learned attractant. Finally, we review the regulation of sexual preference by neuropeptides, oxytocin, vasopressin, and kisspeptin. Through this review, we clarified the contradictions and deficiencies in our current knowledge on the neuroendocrine regulation of sexual preference and sought to present problems requiring further study. Citation: Kondo, Y.; Hayashi, H. Keywords: sexual preference; pheromones; olfactory epithelium; vomeronasal organ; sex steroids; Neural and Hormonal Basis of olfactory nervous system; sexual experience; neuropeptide Opposite-Sex Preference by Chemosensory Signals.