Product Specification Sheet

Recombinant Human 4 (HXK-4/HK4/)

Cat. # HXKX45-R-10 Recombinant purified human HXK-4 protein SIZE: 10 ug

Hexokinase catalyzes the first step of several metabolic pathways by HK4/glucokinase Human (465 aa; protein accession # converting D-hexose to D-hexose-6-P. Hexokinase is an allosteric P35557) Recombinant was expressed in E. coli as His-tag fusion inhibited by its products -6-phosphate. At least 4 protein and purified (95%). Puriifed HK3 is a single, non- related hexokinase isoforms ( HXKI-III; HXK-IV also known as glycosylated protein of ~55 kDa. Glucokinase) have been cloned and characterized. (~100kDa for HXK1-III; HXKIV lacks the N-terminal domains and is Recombinant human HK4/HXK4 protein is supplied in 20mM Tris, ~50 kDa) are outer mitochondrial membrane proteins. The N- pH 8.0 in 10% at 1 mg/ml (see lot specific concn on the terminus, containing the mitochondrial target sequence, and the C- vial). Store at -20oC for long term storage. A carrier protein (BSA or terminal has high among various isoforms. The HSA) can be adeed at 0.1% to improve long term storage. catalytic activity is associated with the C-terminus and other regulatory functions are controlled by the N-terminus. HXK4 (mouse Stability: 6-12 months at –20oC or below. 465 aa, isoforms 1) isoforms are produced by use of alternative promoters. The use of alternative promoters apparently enables the o Shipping: 4 C for solutions and room temp for powder type IV hexokinase to be regulated by insulin in the and glucose in the beta cell. This may constitute an important feedback loop for maintaining glucose homeostasis. HXK4 isoform 2 (missing Recommended Usage 122-138 aa) and HXK4-3 (isoforms 3) containes a different N- terminus from 1-15 aa (MLDDRARMEATKKEK- Recombinant human HK4 can be used as antigen for ELISA >MAMDTTRCGAQLLTL). Alternative names for HXK4. EC or Western. 2.7.1.1, Hexokinase type IV, HK IV, HK4, Glucokinase (Gck). General References: Palma F et al (1996) Mol Cell Biol. Synonyms: 155, 23-29; SCHWAB DA et al (1991) Arch biochem Glucokinase, EC 2.7.1.2, Hexokinase-4, Hexokinase type Biophys. 285, 365-370; Colosimo, A. et al (1996) Cytogenet. IV, HK IV, HK4, Hexokinase-D, GCK, GK, GLK, HHF3, Cell Genet. 74: 187-188; . Furuta, H.(1996) Genomics 36: HKIV, HXKP, MODY2. 206-209; Povey, et al (1975) Ann. Hum. Genet. 38: 407-415

*This product is for In vitro research use only. Source of Antigen, and Antibodies

Recombinant Human HK3/HXK3 sequence Related material available from ADI

MGSSHHHHHH SSGLVPRGSH MLDDRARMEA AKKEKVEQIL Antibodies HXKI-III; AEFQLQEEDL KKVMRRMQKE MDRGLRLETH EEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEEGQWS VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF SFPVRHEDID KGILLNWTKG FKASGAEGNN VVGLLRDAIK RRGDFEMDVV AMVNDTVATM ISCYYEDHQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR MCVNTEWGAF GDSGELDEFL LEYDRLVDES SANPGQQLYE KLIGGKYMGE LVRLVLLRLV DENLLFHGEA SEQLRTRGAF ETRFVSQVES DTGDRKQIYN ILSTLGLRPS TTDCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPSCEITFIE SEEGSGRGAA LVSAVACKKA CMLGQ.

HXKX45-R-10 80108A

Alpha Diagnostic Intl Inc., 6203 Woodlake Center Dr, San Antonio, TX 78244, USA; Email: [email protected] ( (800) 786-5777; (210) 561-9515; Fax: (210) 561-9544; Web Site: www.4adi.com