Anti-Dcr2 (D3188)
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Anti-DcR2 produced in rabbit, affinity isolated antibody Catalog Number D3188 Synonyms: Anti-Decoy Receptor 2; Anti-Trail -R4; Storage/Stability Anti-TRUNDD Antibody can be stored at 2-8 °C for three months and at −20 °C for one year. As with all antibodies, care Product Description should be taken to avoid repeated freeze thaw cycles. Anti-DcR2 is produced in rabbit using as immunogen a Antibodies should not be exposed to prolonged high peptide corresponding to amino acids 249-263 of temperatures. human DcR2 precursor. Product Profile Anti-DcR2 recognizes DcR2 by immunoblotting. Immunoblotting: DcR2 antibody can be used for detection of DcR2 expression by Western blot at Apoptosis or programmed cell death is induced in cells 1 μg/mL. A band of ~36 kDa is detected. by a group of death domain-containing receptors including TNFR1, Fas, DR3, DR4, and DR5. Binding of Note: In order to obtain best results in different ligand to these receptors sends signals that activate techniques and preparations we recommend members of the caspase family of proteases. The determining optimal working concentration by titration signals ultimately cause the degradation of test. chromosomal DNA by activating DNase. DR5 and DR4 are closely related members of the TNF family of death- References domain containing receptors. These proteins share 1. Singh, et al., Death domain receptors and their role homologies in both their extracellular ligand binding in cell demise. J. Interferon Cytokine Res., 18, 439- domains and their intracellular effector domains (death 450 (1998). domains). These death domains are ~80 amino acids 2. Pan G, et al., The receptor for the cytotoxic ligand long.1 Both DR4 and DR5 bind TRAIL. This binding TRAIL. Science, 276, 111-113 (1997). induces apoptosis.2 DcR2 is a decoy receptor that 3. Marsters SA, et al., A novel receptor for binds to TRAIL with its extracellular TRAIL-binding Apo2L/TRAIL contains a truncated death domain. domain, but it does not have an intracellular death Curr. Biol., 7, 1003-1006 (1997). domain and, therefore, does not induce apoptosis.3-6 4. Degli-Esposti MA, et al., The novel receptor DcR2 is found in most human tissue, and its over- TRAIL-R4 induces NF-B and protects against expression reduces TRAIL-induced apoptosis. TRAIL-mediated apoptosis, yet retains an Another decoy TRAIL receptor, DcR1, has also been incomplete death domain. Immunity, 7, 813-820 identified.7 Like DcR2 it lacks the death domain and (1997). inhibits TRAIL signaling. 5. Pan G, et al., TRUNDD, a new member of the TRAIL receptor family that antagonizes TRAIL Reagents signalling. FEBS Lett., 424, 41-45 (1998). Supplied at ~0.5 mg/ml in phosphate buffered saline, 6. Pan G, et al., An antagonist decoy receptor and a containing 0.02% sodium azide death domain-containing receptor for TRAIL. Science 277, 815-818 (1997). Precautions and Disclaimer 7. Sheridan, J.P., et al., Control of TRAIL-induced This product is for R&D use only, not for drug, apoptosis by a family of signaling and decoy household, or other uses. Please consult the Safety receptors. Science, 277, 818-821 (1997). Data Sheet for information regarding hazards and safe handling practices. SG,PHC 10/16-1 2016 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip. .