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Genetic Tool Development in Marine Protists bioRxiv preprint doi: https://doi.org/10.1101/718239; this version posted April 8, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 1 Genetic tool development in marine protists: 2 Emerging model organisms for experimental cell biology 3 4 Drahomíra Faktorová1,#,*, R. Ellen R. Nisbet2,#, %, José A. Fernández Robledo3,#, Elena 5 Casacuberta4,#, Lisa Sudek5,#, Andrew E. Allen6,7, Manuel Ares Jr.8, Cristina Aresté4, Cecilia 6 Balestreri9, Adrian C. Barbrook2, Patrick Beardslee10, Sara Bender11, David S. Booth12, 7 François-Yves Bouget13, Chris Bowler14, Susana A. Breglia15, Colin Brownlee9, Gertraud 8 Burger16, Heriberto Cerutti10, Rachele Cesaroni17, Miguel A. Chiurillo18, Thomas Clemente10, 9 Duncan B. Coles3, Jackie L. Collier19, Elizabeth C. Cooney20, Kathryn Coyne21, Roberto 10 Docampo18, Christopher L. Dupont7, Virginia Edgcomb22, Elin Einarsson2, Pía A. 11 Elustondo15,$, Fernan Federici23, Veronica Freire-Beneitez24,25, Nastasia J. Freyria3, Kodai 12 Fukuda26, Paulo A. García27, Peter R. Girguis28, Fatma Gomaa28, Sebastian G. Gornik29, Jian 13 Guo5,8, Vladimír Hampl30, Yutaka Hanawa31, Esteban R. Haro-Contreras15, Elisabeth 14 Hehenberger20, Andrea Highfield9, Yoshihisa Hirakawa31, Amanda Hopes32, Christopher J. 15 Howe2, Ian Hu2, Jorge Ibañez23, Nicholas A.T. Irwin20, Yuu Ishii33, Natalia Ewa Janowicz30, 16 Adam C. Jones11, Ambar Kachale1, Konomi Fujimura-Kamada34, Binnypreet Kaur1, Jonathan 17 Z. Kaye11, Eleanna Kazana24,25, Patrick J. Keeling20, Nicole King12, Lawrence A. 18 Klobutcher35, Noelia Lander18, Imen Lassadi2, Zhuhong Li18, Senjie Lin35, Jean-Claude 19 Lozano13, Fulei Luan10, Shinichiro Maruyama33, Tamara Matute23, Cristina Miceli36, Jun 20 Minagawa34,37, Mark Moosburner6,7, Sebastián R. Najle4,38, Deepak Nanjappa21, Isabel C. 21 Nimmo2, Luke Noble39,**, Anna M.G. Novák Vanclová30, Mariusz Nowacki17, Isaac Nuñez23, 22 Arnab Pain40,41, Angela Piersanti36, Sandra Pucciarelli36, Jan Pyrih24,30, Joshua S. Rest42, 23 Mariana Rius19, Deborah Robertson43, Albane Ruaud23,***, Iñaki Ruiz-Trillo4,44,45, Monika A. 24 Sigg12, Pamela A. Silver46,47, Claudio H. Slamovits15, G. Jason Smith48, Brittany N. 25 Sprecher35, Rowena Stern9, Estienne C. Swart17,***, Anastasios D. Tsaousis24,25, Lev 1 bioRxiv preprint doi: https://doi.org/10.1101/718239; this version posted April 8, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 26 Tsypin49,50, Aaron Turkewitz49, Jernej Turnšek6,7,46,47, Matus Valach16, Valérie Vergé13, Peter 27 von Dassow23,51, Tobias von der Haar24, Ross F. Waller2, Lu Wang52, Xiaoxue Wen10, Glen 28 Wheeler9, April Woods48, Huan Zhang35, Thomas Mock32,*, Alexandra Z. Worden5,53,* & 29 Julius Lukeš1,* 30 31 1Institute of Parasitology, Biology Centre, Czech Academy of Sciences and Faculty of 32 Sciences, University of South Bohemia, České Budějovice, Czech Republic 33 2Department of Biochemistry, University of Cambridge, Cambridge, UK 34 3Bigelow Laboratory for Ocean Sciences, East Boothbay, USA 35 4Institut de Biologia Evolutiva, CSIC-Universitat Pompeu Fabra, Barcelona, Spain 36 5Monterey Bay Aquarium Research Institute, Moss Landing, USA 37 6Integrative Oceanography Division, Scripps Institution of Oceanography, University of 38 California, San Diego, USA 39 7Microbial and Environmental Genomics, J. Craig Venter Institute, La Jolla, USA 40 8Molecular, Cell and Developmental Biology, University of California, Santa Cruz, USA 41 9The Marine Biological Association, Plymouth and School of Ocean and Earth Sciences, 42 University of Southampton, Southampton, UK 43 10School of Biological Sciences, University of Nebraska, Lincoln, USA 44 11Gordon and Betty Moore Foundation, Palo Alto, USA 45 12Department of Molecular and Cell Biology, University of California, Berkeley, USA 46 13Sorbonne Université, CNRS UMR7621, Observatoire Océanologique, Banyuls sur Mer, 47 France 48 14Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, 49 CNRS, INSERM, Université PSL, Paris, France 2 bioRxiv preprint doi: https://doi.org/10.1101/718239; this version posted April 8, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 50 15Centre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie 51 University, Halifax, Canada 52 16Department of Biochemistry and Robert-Cedergren Centre for Bioinformatics and 53 Genomics, Université de Montréal, Montreal, Canada 54 17Institute of Cell Biology, University of Bern, Bern, Switzerland 55 18Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, USA 56 19School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, USA 57 20Department of Botany, University of British Columbia, Vancouver, Canada 58 21University of Delaware College of Earth, Ocean and Environment, Lewes, USA 59 22Woods Hole Oceanographic Institution, Woods Hole, USA 60 23Facultad Ciencias Biológicas, Pontificia Universidad Católica de Chile, Fondo de 61 Desarrollo de Areas Prioritarias, Center for Genome Regulation and Millennium 62 Institute for Integrative Biology (iBio), Santiago de Chile, Chile 63 24School of Biosciences, University of Kent, Canterbury, Kent, UK 64 25Laboratory of Molecular and Evolutionary Parasitology, University of Kent, UK 65 26Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, 66 Japan 67 27Department of Mechanical Engineering, Massachusetts Institute of Technology, Boston, 68 USA 69 28Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, 70 USA 71 29Centre for Organismal Studies, University of Heidelberg, Heidelberg, Germany 72 30Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, 73 Czech Republic 31 74 Faculty of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan 3 bioRxiv preprint doi: https://doi.org/10.1101/718239; this version posted April 8, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 75 32School of Environmental Sciences, University of East Anglia, Norwich, UK 76 33Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan 77 34Division of Environmental Photobiology, National Institute for Basic Biology, Okazaki, 78 Aichi, Japan 79 35Department of Marine Sciences, University of Connecticut, Groton, USA 80 36School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy 81 37Department of Basic Biology, School of Life Science, Graduate University for Advanced 82 Studies, Okazaki, Aichi, Japan 83 38Instituto de Biología Molecular y Celular, CONICET, and Facultad de Ciencias 84 Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina 85 39Center for Genomics and Systems Biology, New York University, New York, USA 86 40Biological and Environmental Science and Engineering Division, King Abdullah 87 University of Science and Technology, Thuwal, Saudi Arabia 88 41Center for Zoonosis Control, Global Institution for Collaborative Research and 89 Education, Hokkaido University, Sapporo, Japan 90 42Department of Ecology and Evolution, Stony Brook University, Stony Brook, USA 91 43Lasry Center for Biosciences, Clark University, Worcester, USA 92 44Departament de Genètica Microbiologia i Estadıśtica, Universitat de Barcelona, 93 Barcelona, Spain 94 45Catalan Institution for Research and Advanced Studies, Barcelona, Spain 95 46Department of Systems Biology, Harvard Medical School, Boston, USA 96 47Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, USA 97 48Department of Environmental Biotechnology, Moss Landing Marine Laboratoriess, Moss 98 Landing, USA 4 bioRxiv preprint doi: https://doi.org/10.1101/718239; this version posted April 8, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 99 49Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, 100 USA 101 50Department of Biology, California Institute of Technology, Pasadena, USA 102 51Instituto Milenio de Oceanografia de Chile, Concepción, Chile 103 52Institute of Oceanography, Minjiang University, Fuzhou, China 104 53Ocean EcoSystems Biology Unit, Marine Ecology Division, Helmholtz Centre for Ocean 105 Research, Kiel, Germany 106 107 % Present address: School of Biosciences, University of Nottingham, Sutton Bonington, UK 108 $Present address: AGADA Biosciences Inc., Halifax, Canada 109 **Present address: Institute de Biologie de l’ENS, Département de biologie, École Normale 110 Supérieure, CNRS, INSERM, Paris, France 111 ***Present address: Max Planck Institute for Developmental Biology, Tübingen, Germany 112 113 #Equal contribution 114 115 *Corresponding authors: [email protected]
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