CHI HEALTH LABORATORY REFERENCE CHART FOR MICROBIOLOGY SPECIMEN COLLECTION

EPIC ORDER TRANSPORT TEST COLLECTION DEVICES SPECIMEN TIME AND TEMPERATURE AFB Culture and Sterile Container Refrigerate Smear LAB877 Sputum, BAL, Min 2 mL Fluid: Urine, and Body AFB CULTURE Sputum, CSF, Fluids (INCLUDES SMEAR) BAL, Urine, Body Stable:72 hr. Fluid CSF must be

Ambient Stable:<24 hr. AFB Culture, blood SPS Vacutainer tube Blood and Bone AFB BLOOD LAB246 Blood or Bone Marrow must be CULTURE Marrow Ambient Stable:24 hr VAGINAL DNA Vaginal Pathogen Affirm Kit PROBE DNA probe Sterile Swab, Collection Tube, Ambient or Vaginal (Detection of LAB3001 Transport Reagent Dropper Refrigerated Specimen Candida/Gardnerella/ Stable:<72 hr. Trichomonas) Tissue Culture Cultures-Sterile Container Send ASAP to LAB898 lab. If delay is If small specimen expected, add a BIOPSIES, FNA, add sterile saline. small amount of Node, Nodule, Polyp, Never wrap in sterile saline to Tissue CULTURE gauze tissue to prevent drying. Ambient 24 hr Blood Culture Bactec® LAB462 Bactec® Lytic 10 Plus+ Blood 10 ml into Ambient BLOOD CULTURES Anaerobic/ Aerobic/F each bottle Stable:48 hr. Blue Lid F Purple Lid

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EPIC ORDER TRANSPORT TEST COLLECTION DEVICES SPECIMEN TIME AND TEMPERATURE Bordetella Nasopharyngeal Freeze pertussis/ Mini Swab Stable: 3 months BORDETELLA Parapertussis by PERTUSSIS PCR PCR LAB923 (discard large UVTM swab) Universal Viral Transport Media Mini-tip ESwab Bacterial Culture Chlamydia by PCR Dirty Urine First (Urine) LAB5763 Morning Void (No clean catch CHLAMYDIA Chlamydia/ or midstream Stable at 2-30° TRACHOMATIS by Gonorrhea by PCR Cobas® Urine PCR Media tube urine). Less C for 12 months PCR (URINE) (Urine) LAB5761 than 50 mL. Male or Female

Chlamydia/ For CERVICAL Gonorrhea by PCR collection use (Genital Swab) only one swab. LAB5757 First remove mucous with one Chlamydia/ swab and Gonorrhea by PCR discard. Then Ambient or CHLAMYDIA (Thinprep) Thin Prep vial collect. refrigerated TRACHOMATIS by LAB5758 Stable 12 months PCR TESTING

(GENITAL) Chlamydia by PCR

(Genital Swab) LAB5756 Cobas® Female PCR media tube Endocervical or Vaginal Chlamydia by PCR (Thinprep) LAB5760

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EPIC ORDER TRANSPORT TEST COLLECTION DEVICES SPECIMEN TIME AND TEMPERATURE Chlamydia Non-genital Specimens. (eye, Submit one trachomatis culture nose, throat, anal) swab in vial. (2 LAB244 Children less than 13 y/o swabs in kit: one CHLAMYDIA Legal cases big and one Refrigerate TRACHOMATIS (CT) Stable: 72 hr. CULTURE small for different Universal Viral Transport Media collection sites)

CLOSTRIDIUM Clostriduim difficile Clean Vial Loose, Diarrheal DIFFICLE by PCR toxin LAB253 Stool or Colon Stable: Aspirate Refrigerated 5 Once every 7 days days.

Formed stools will not be tested.

Giardia/Crypto SAF Vial Screen LAB2253 Fecal or Colon Aspirate; must be CRYPTOSPORIDIUM put in Ambient /GIARDIA ANTIGEN preservative Stable: 7 days within 2 hours of collection

Anaerobic Cutlure eSwab® Sterile container: LAB233 Acceptable Room temp specimens: Stable: 2 hr. CULTURE FOR tissue or body ANAEROBES fluid including eSwab® Room abcess, sinus or Temp Sterile Container for tissue middle meatus Stable: 48 hr. or body fluid

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EPIC ORDER TRANSPORT TEST COLLECTION DEVICES SPECIMEN TIME AND TEMPERATURE Culture, Routine LAB899 eSwab® Room CULTURE ROUTINE List Specific Temp FOR BACTERIA Source Stable: 48 hr. . eSwab® Mini-tip ESwab Bacterial Culture Ear Culture with gram stain LAB942 eSwab® Room EAR DISCHARGE Ear aspirate or Temp CULTURE ear drainage Stable: 48 hr. Sterile Container eSwab®

Eye culture with For Corneal scrapings must gram stain LAB943 plate directly onto blood and eSwab® Room Temp chocolate agar and into Conjunctival Stable: 48 hr. EYE CULTURE thioglycolate broth. Place drainage or

material for gram stain on a corneal scraping glass slide and air dry. Plates: incubate eSwab® for Conjunctival drainage within 2 horus

Fecal Leukocytes LAB265

FECAL Stool or Colon Refrigerate Clean Vial LEUKOCYTES Aspirate Stable: 2 weeks

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EPIC ORDER TRANSPORT TEST COLLECTION DEVICES SPECIMEN TIME AND TEMPERATURE Stool Culture and *Enteric Plus, C & S gram stain LAB223 Clean Vial *Preferred Specimen Enteric Plus- STOOL CULTURE – Fresh Stool or Colon Ambient (FOR ENTERIC Must be at Aspirate Stable: 96 hr PATHOGENS) LAB within 2 hrs of collection . Body fluid culture Sterile Conical Tube Aspirate of and smear LAB269 abdominal, amniotic, Send to Lab ascites, bile, ASAP FLUIDS joint, pericardial, Ambient peritoneal, Stable: 24 hr pleural, synovial Sterile Cup and vitreous fluids Fungal Derm Sterile Container containing Culture LAB4193 nails, skin scrapings, or hair List Specific Refrigerate FUNGAL DERM Source. Stable: 72 hr CULTURE

Fungal culture, SPS Vacutainer tube Blood and Bone FUNGAL BLOOD blood LAB242 Blood or Bone Marrow must be CULTURE Marrow Ambient Stable:24 hr

Giardia/Crypto Fecal or Colon Aspirate; must be GIARDIA / Screen LAB2253 put in Ambient CRYPTOSPORIDIUM preservative Stable: 7 days ANTIGEN within 2 hours of collection SAF Vial

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EPIC ORDER TRANSPORT TEST COLLECTION DEVICES SPECIMEN TIME AND TEMPERATURE Gonococcus Culture LAB235 Non-genital Specimens. (eye, nose, throat, anal) eSwab® (NEISSERIA) Children less Ambient GONORRHEA (NG) than 13 y/o Stable:24 hr. CULTURE Legal cases eSwab®

Chlamydia/ For CERVICAL Gonorrhea by PCR collection use (Thinprep) only one swab. LAB5758 First remove mucous with one Chlamydia/ swab and (NEISSERIA) Gonorrhea by PCR Thin Prep Vial discard. Then Ambient or GONORRHOEAE (Genital Swab) collect. refrigerated (NG) by PCR LAB5757 Stable 12 months TESTING (Genital) Gonorrhea by PCR

(Genital Swab) Cobas® Female PCR media tube LAB5755 Endocervical or Vaginal

Gonorrhea by PCR (Thin Prep) LAB5759

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EPIC ORDER TRANSPORT TEST COLLECTION DEVICES SPECIMEN TIME AND TEMPERATURE Gonorrhea by PCR Dirty Urine First (Urine) LAB5762 Morning Void (NEISSERIA) (No clean catch GONORRHOEAE Chlamydia/ or midstream Stable at 2-30° (NG) by PCR Cobas® Urine PCR Media tube Gonorrhea by PCR urine). Less C for 12 months TESTING (Urine) LAB5761 than 50 mL. (URINE) Male or Female

H. pylori antigen, stool (aka Stable: HELICOBACTER) Refrigerated HELICOBACTER 72 hr LAB397 Clean Vial Stool PYLORI ANTIGEN Stable: Frozen 30 days

Herpes simplex Swabs from virus 1&2 by PCR cutaneous and LAB2398 mucocutaneous HERPES SIMPLEX Universal Viral Transport Media Refrigerate lesions VIRUS 1 & 2 BY PCR (UVT) Stable: 1 week

CSF- minimum 2ml Influenza type A/B and RSV LAB2432 Nasopharyngeal Refrigerate INFLUENZA A/B AND Universal Viral Transport Media swab in UVT UVT Stable:7 RSV BY PCR (UVT) days

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EPIC ORDER TRANSPORT TEST COLLECTION DEVICES SPECIMEN TIME AND TEMPERATURE Legionella species Universal Viral Transport Media Respiratory by PCR LAB2451 (UVT) specimen: BAL, bronchial brushings, LEGIONELLA Refrigerate nasopharyngeal SPECIES BY PCR Stable: 5 days swab, sputum, Sterile Container tracheal aspirates or pleural fluid Legionella Antigen, Urine LAB886 LEGIONELLA Refrigerate Legionella Sterile Container Urine (min 1 mL) ANTIGEN Stable:2 weeks

Malaria Smear Blood Slides MALARIA SMEARS; LAB883 Whole Blood min: 1 mL Have collected at Ambient BLOOD PARASITE Thick and thin smear slides Lab; slides must Stable several SCREEN must be made immediately after be made within 1 EDTA 4 mL tube (Blood) days collection hour of collection GENITAL Mycoplasma/ MYCOPLASMA Ureaplasma culture CULTURE LAB944 Urogenital (place Universal Viral Transport Media Refrigerate (culture for swab directly in (UVT) Stable: 48 hr.?? Mycoplasma hominis UVT) and Ureaplasma

species) Occult blood 1-3, stool LAB694 OCCULT BLOOD, Fecal or Colon Refrigerate STOOL Aspirate Stable:7 days

Clean Vial

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EPIC ORDER TRANSPORT TEST COLLECTION DEVICES SPECIMEN TIME AND TEMPERATURE Occult Blood gastric/duodenum LAB696 OCCULT BLOOD, 1 mL gastric Refrigerate GASTRIC sample Stable: 24 hr

Clean Vial Ova and Parasite Testing is ONLY appropriate Examination when the patient has diarrhea LAB955 and one of the following conditions: patient has traveled to countries with endemic SAF Fecal or Colon parasitic infestation, patient is a Stable: Ambient 1 Aspirate OVA & PARASITES resident or an immigrant from a month

developing country, or patient

has unexplained eosinophilia. SAF Vial Three samples collected over a 10 day period are optimum. Indicate suspected parasite & travel history. Pinworm Prep Collect first thing in the AM right LAB248 after patient wakes up. Remove the cap from the pinworm collector. In the cap is inserted a paddle, one side of which is coated with an adhesive Anal/Perianal material. This side is marked Refrigerate PIN WORM Pinworm sticky side. Don't touch this Stable:24 hr. Collection Kit surface with the fingers. Pin Worm Paddle or SCOTCH TAPE Separate the buttocks and press the sticky side to the outside of the anus. Insert paddle with cap attached back in to the pinworm collector.

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EPIC ORDER TRANSPORT TEST COLLECTION DEVICES SPECIMEN TIME AND TEMPERATURE RSV Screen Nasopharyngeal LAB495 swab in UVT or RSV (RESPIRATORY Nasopharyngeal SYNCYTIAL VIRUS) wash UVT Stable: 72 ANTIGEN Only indicated for hr. Nasal Washing in patients under Universal Viral Transport Media Sterile container the age of 5. Rotavirus Antigen, stool LAB443

ROTAVIRUS Refrigerate Stool ANTIGEN Stable: 3 days

Clean Container Sputum Culture LAB900 Expectorated Refrigerate SPUTUM CULTURE Sterile Container Sputum or Stable:24 hr. Trachea Aspirate

Rapid Strep Screen Mini-tip ESwab Bacterial LAB885 Culture Ambient Stable: 24 hr STREP GROUP A Throat Refrigerate ANTIGEN, RAPID Stable: 48 hr eSwab®

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EPIC ORDER TRANSPORT TEST COLLECTION DEVICES SPECIMEN TIME AND TEMPERATURE Strep A Culture, Mini-tip ESwab Bacterial Throat LAB236 Culture

Ambient eSwab® STREP A CULTURE Throat Stable: 48 hr eSwab®

Throat Culture Mini-tip ESwab Bacterial LAB228 Culture Ambient eSwab® Stable: THROAT CULTURE Throat/Tonsil 48 hr eSwab®

Wet prep, genital vaginal discharge TRICHOMONAS (aka A swab in 1 ml saline (Place swab in Stable 30 WET PREP TRICHOMONAS) saline; examine minutes. LAB252 immediately) Urinalysis Males: first voided urine (not microscopic clean/not midstream) TRICHOMONAS LAB3210 with Urine Ambient < 4 hr (MALES) comment Look for Trichomonas

Urine Culture Cup: Stable: LAB239 Refrigerated <24 hr. Urine Clean URINE CULTURE Catch TT Stable: Ambient or Sterile urine cup Urine C&S Transport Tube(TT) Refrigerated 48 hr

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EPIC ORDER TRANSPORT TEST COLLECTION DEVICES SPECIMEN TIME AND TEMPERATURE Group B Strep Mini-tip ESwab Bacterial Culture LAB1377 Culture Ambient VAGINAL GRP B Vaginal eSwab® CULTURE Stable:48 hr. eSwab®

Culture Viral Reflex LAB4032 Refrigerated Sterile Container List specific VIRAL CULTURE Stable: <4 hr. source

Universal Viral Transport Media for Sterile Container for UVTStable 72 hr. Lesions and Nasopharyngeal Fluids, Feces, Nasal Washings, collection Urine, or Biopsy Routine Culture Mini-tip ESwab Bacterial LAB899 Culture Ambient ESwab Stable: 48 List specific WOUND CULTURE hr. source

ESwab

Yeast Culture Mini-tip ESwab Bacterial LAB241 Culture Ambient YEAST (CANDIDA List specific ESwab Stable: 48 SCREEN) source hr.

ESwab

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