DOCSLIB.ORG

Fotografía De Un Espeleotema De La Cueva De Castañar De Ibor (Extremadura)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Fotografía De Un Espeleotema De La Cueva De Castañar De Ibor (Extremadura) Todos los derechos reservados. La totalidad o una parte de este libro no puede ser reproducida o utilizada en cualquier forma o medio, electrónico o mecánico, incluyendo copias o grabaciones o por cualquier medio de almacenar información y sistema de recuperación, sin el previo permiso por escrito del IRNAS‐CSIC. © Estefanía Porca Belío Diseño y maquetación: Estefanía Porca Belío. Portada: Fotografía de un espeleotema de la Cueva de Castañar de Ibor (Extremadura). Diseño de Miguel Ángel Rogerio Candelera y Estefanía Porca Belío. Editado por: Instituto de Recursos Naturales y Agrobiología de Sevilla, IRNAS‐CSIC, España, Noviembre 2011 I.S.B.N: 978‐84‐695‐0670‐7 Impreso en España‐ Printed in Spain Aerobiología: mecanismos de dispersión de los microorganismos en cuevas turísticas. Memoria que presenta la Licenciada en Biología Dña. ESTEFANÍA PORCA BELÍO para optar al título de Doctor Europeo en Biología por la Universidad de Sevilla. Sevilla 2011 Memoria que presenta la Licenciada en Biología Dña. ESTEFANÍA PORCA BELÍO para optar al título de Doctor Europeo en Biología por la Universidad de Sevilla. Aerobiología: mecanismos de dispersión de los microorganismos en cuevas turísticas. Visado en Sevilla, a 11 de Noviembre de 2011 LOS DIRECTORES Prof. Dr. D. CESÁREO SÁIZ JIMÉNEZ Dra. Dña. VALME JURADO Profesor de Investigación en el Instituto de Doctora contratada en el Instituto Recursos Naturales y Agrobiología de Sevilla de Recursos Naturales y Agrobiología (IRNAS‐CSIC) de Sevilla (IRNAS‐CSIC). LA TUTORA Dra. Dña. CAROLINA SOUSA MARTÍN Profesora Titular de la Universidad de Sevilla Departamento de Microbiología y Parasitología, Facultad de Farmacia. PROF. DR. D. CESÁREO SÁIZ JIMÉNEZ, PROFESOR DE INVESTIGACIÓN DEL INSTITUTO DE RECURSOS NATURALES Y AGROBIOLOGÍA DE SEVILLA DEL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS (IRNAS‐CSIC) Y DRA. DÑA. VALME JURADO LOBO, DOCTORA CONTRATADA CERTIFICA: Que la presente Memoria de Investigación titulada ”Aerobiología: mecanismos de dispersión de los microorganismos en cuevas turísticas”, presentada por la Lcda. en Biología ESTEFANÍA PORCA BELÍO para optar al grado de Doctor Europeo en Biología, ha sido realizada en el Departamento de Geoecología, Biogeoquímica y Microbiología Ambiental (IRNAS‐CSIC), bajo nuestra dirección reuniendo todos los requisitos exigidos. Y para que así conste, se expide y firma el presente certificado. En Sevilla, a 11 de Noviembre de 2011 Fdo.: Prof. Dr. D. Cesáreo Sáiz Jiménez Fdo.: Dra. Dña. Valme Jurado Lobo DR. D. JOSÉ MANUEL PARDO PRIETO DIRECTOR DEL INSTITUTO DE RECURSOS NATURALES Y AGROBIOLOGÍA DE SEVILLA DEL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS (IRNAS‐CSIC) CERTIFICA: Que la presente Memoria de Investigación titulada “Aerobiología: mecanismos de dispersión de los microorganismos en cuevas turísticas”, presentada por la Lcda. en Biología ESTEFANÍA PORCA BELÍO para optar al grado de Doctor Europeo en Biología, ha sido realizada en el Departamento de Geoecología, Biogeoquímica y Microbiología Ambiental (IRNAS‐CSIC), bajo la dirección del Prof. Dr. D. CESÁREO SÁIZ JIMÉNEZ y la Dra. Dña. VALME JURADO LOBO, reuniendo todas las condiciones exigidas a los trabajos de Tesis Doctorales. Y para que así conste, se expide y firma el presente certificado. En Sevilla, a 11 de Noviembre de 2011 Fdo.: Dr. D. José Manuel Pardo Prieto Agradezco... Al Prof. Dr. Sáiz Jiménez y a la Dra. Jurado por todo lo que me han enseñado durante estos cuatro años, y el esfuerzo y dedicación que ellos también han puesto en esta tesis. A todos los compañeros del grupo del Dr. D. Cesáreo Sáiz, pero muy, muy especialmente a Valme e Isabel, os llevaré siempre en mi corazón. A las supervisoras de los grupos donde he ido de estancia, por el tiempo que me han dedicado y todo lo que me han enseñado, la Dra. Rebekka Artz (The Macaulay Land Use Research Institute, Escocia), Dra. Alena Nováková (Institute of Soil Biology, Repúbica Checa), Dra. Fabiola Bastián (INRA/ Université de Bourgone, Francia) y la Dra. Pašić (University of Ljubljana, Eslovenia). Al Dr. D. José Díaz (Universidade da Coruña) que fue el primero que creyó en mí y me dio la oportunidad de empezar y descubrir este apasionante mundo de la investigación. A Aitana, Andrea, Marta, Miriam, Julia, etc. por su cariño y apoyo en todo momento. Muy especialmente agradecer a mi familia su apoyo y comprensión incondicional, sin olvidarme de mi abuelo Antonio. A la beca predoctoral de la Junta de Ampliación de Estudios (JAE) del Consejo Superior de Investigaciones Científicas (CSIC) gracias a la cual ha sido posible realizar esta tesis. La financiación proporcionada por los siguientes proyectos de investigación: Investigación en Tecnologías para la conservación y valorización del Patrimonio Cultural (TCP) (CSD2007‐00058). Ministerio de Ciencia e Innovación. Écologie Microbienne de la Grotte de Lascaux. Ministerio de Cultura y de la Comunicación de Francia. Observatorio microbiológico de cuevas visitables: evaluación y control de comunidades fúngicas en cuevas sometidas al impacto de actividades turísticas (RNM‐5137). Consejería de Innovación, Ciencia y Empresa (Junta de Andalucía). A todo el personal de las cuevas estudias, Castañar de Ibor, Altamira, Ardales y Lascaux por la disposición y facilidades prestadas a la hora de realizar los muestreos. Parte de los resultados obtenidos han sido incluidos en dos informes científicos sobre la cueva de Altamira y la de Lascaux presentados a los Ministerios de Cultura Español y Francés. Además se han obtenido las siguientes publicaciones científicas relacionadas con este trabajo: Saiz‐Jimenez, C., Cuezva, S., Jurado, V., Fernández‐Cortés, A., Porca, E., Benavente, D., Cañaveras, J.C. y Sánchez‐Moral, S. (2011). Paleolithic Art in Peril: Policity and Science Collide at Altamira Cave. Science 334: 42‐43. Martín‐Sánchez, P.M., Bastián, F., Nováková, A., Porca, E., Jurado, V., Sánchez‐Cortés, S., López‐ Tobar, E., García‐ Sánchez, A., Ariza, C., Hernández‐Mariné, M., Alabouvette, C. y Saiz‐Jimenez, C. (2011). Écologie Microbienne de la Grotte de Lascaux. IRNAS‐CSIC, Spain. I.S.B.N. 978‐84‐694‐7852‐3. Fernández‐Cortés, A., Cuezva, S., Sánchez‐Moral, S., Porca, E., Jurado, V., Martín‐Sánchez, P.M. y Saiz‐Jimenez, C. (2011). Detection of human‐induced environmental disturbance in a subterranean environment: microclimatic and aerobiological monitoring. Environmental Science and Pollution Research 18: 1037‐1045. Porca, E., Jurado, V., Martín‐Sánchez, P.M., Hermosin, B., Bastián, F., Alabouvette, C. y Saiz‐Jimenez, C. (2011). Aerobiology: an ecological indicator for early detection and control of fungal outbreaks in caves. Ecological Indicators 11: 1594‐1598 Jurado, V., Porca, E., Cuezva, S., Fernández‐Cortés, A., Sánchez‐Moral, S. y Saiz‐Jimenez, C. (2010). Fungal outbreak in a show cave. Science of the Total Environment 408: 3632‐ 3638. ÍÍÍnnndddiiiccceee dddeee CCCooonnnttteeennniiidddooosss Capítulo 1: Introducción 1.1‐ CUEVAS………………………………………………………………………………………… 1 1.2‐ DIVERSIDAD MICROBIANA EN CUEVAS………………………………………… 12 1.3‐ AEROBIOLOGÍA…………………………………………………………………………….. 22 Capítulo 2: Antecedentes y Objetivos 2.1‐ ANTECEDENTES……………………………………………………………………………. 33 2.2‐ OBJETIVOS……………………………………………………………………………………. 38 Capítulo 3: Descripción de las Cuevas Estudiadas 3.1‐ CUEVA DE CASTAÑAR DE IBOR (EXTREMADURA)………………………….. 41 3.2‐ CUEVA DE ARDALES (ANDALUCÍA)………………………………………………… 49 3.3‐ CUEVA DE ALTAMIRA (CANTABRIA)……………………………………………… 58 3.4‐ CUEVA DE LASCAUX (FRANCIA)…………………………………………………….. 71 Capítulo 4: Material y Métodos 4.1‐ MUESTREO Y ANÁLISIS DE LAS MUESTRAS……………………………………. 83 4.1.1‐ DUO SAS SUPER 360……………………………………………………………. 84 4.1.2‐ CORIOLIS μ…………………………………………………………………………… 95 4.2‐ LOCALIZACIÓN DE LAS MUESTRAS………………………………………………… 101 4.2.1‐ CUEVA DE CASTAÑAR DE IBOR (EXTREMADURA)…………………. 102 4.2.2‐ CUEVA DE ARDALES (ANDALUCÍA)……………………………………….. 104 4.2.3‐ CUEVA DE ALTAMIRA (CANTABRIA)……………………………………… 105 4.2.4‐ CUEVA DE LASCAUX (FRANCIA)……………………………………………. 107 4.3‐ MEDIOS DE CULTIVO Y SOLUCIONES…………………………………………….. 108 4.3.1‐ MEDIOS DE CULTIVO……………………………………………………………. 108 4.3.2‐ SOLUCIONES………………………………………………………………………… 109 Capítulo 5: Resultados y Discusión 5.1‐ CUEVA DE CASTAÑAR DE IBOR (EXTREMADURA)………………………….. 114 5.1.1 Muestreador DUO SAS SUPER 360………………………………………… 114 5.1.1.1‐ DIVERSIDAD DE HONGOS……………………………………………. 114 5.1.1.2‐ DIVERSIDAD DE BACTERIAS…………………………………………. 121 5.1.2 Muestreador Coriolis μ…………………………………………………………. 128 5.1.2.1‐ DIVERSIDAD DE HONGOS……………………………………………. 128 5.1.2.2‐ DIVERSIDAD DE BACTERIAS…………………………………………. 133 5.2‐ CUEVA DE ARDALES (ANDALUCÍA)………………………………………………… 140 5.2.1 Muestreador DUO SAS SUPER 360………………………………………… 140 5.2.1.1‐ DIVERSIDAD DE HONGOS……………………………………………. 140 5.2.1.2‐ DIVERSIDAD DE BACTERIAS…………………………………………. 145 5.3‐ CUEVA DE ALTAMIRA Y ESTALACTITAS 154 (CANTABRIA)……………………………………………………………………………………….. 5.3.1 Muestreador DUO SAS SUPER 360………………………………………… 154 5.3.1.1‐ DIVERSIDAD DE HONGOS……………………………………………. 154 5.3.1.2‐ DIVERSIDAD DE BACTERIAS…………………………………………. 159 5.3.2 Muestreador Coriolis μ…………………………………………………………. 165 5.3.2.1‐ DIVERSIDAD DE HONGOS……………………………………………. 165 5.3.2.2‐ DIVERSIDAD DE BACTERIAS…………………………………………. 169 5.4‐ CUEVA DE LASCAUX (FRANCIA)…………………………………………………….. 176 5.4.1 Muestreador DUO SAS SUPER 360………………………………………… 176 5.4.1.1‐ DIVERSIDAD DE HONGOS……………………………………………. 176 5.4.1.2‐ DIVERSIDAD DE BACTERIAS…………………………………………. 186 5.5‐ VENTAJAS E INCONVENIENTES DE LOS DOS MUESTREADORES DE 196 AIRE EMPLEADOS EN ESTA TESIS…………………………………………………… 5.6‐ CONSIDERACIONES GENERALES…………………………………………………….
Load more

Recommended publications
  • The Unseen Majority”: Heterotrophic Bacteria in Freshwater, More Than Just Small and Non-Cultivable
    Eawag_07258 Diss. ETH No. 17894 “The unseen majority”: heterotrophic bacteria in freshwater, more than just small and non-cultivable A dissertation submitted to ETH ZÜRICH for the degree of Doctor of Sciences presented by Yingying Wang MPhil, The University of Hong Kong born March 16, 1980 in Tianjin citizen of China accepted on the recommendation of Prof. Dr. Thomas Egli, examiner Dr. Frederik Hammes, co-examiner Prof. Dr. Nico Boon, co-examiner Prof. Dr. Jakob Pernthaler, co-examiner Prof. Dr. Martin Ackermann, co-examiner July, 2008 Acknowledgement I am extremely grateful to Prof. Dr. Thomas Egli for his great supervision, invaluable advice, and providing many academic opportunities for me to widen and enrich my knowledge, vision and experience. I am also full of gratitude to Dr. Frederik Hammes for generously sharing his invaluable research experience, and for numerous inspirational discussions. Their professional and excellent guidance during my doctoral study and academic development are highly appreciated. My special thanks go to Prof. Dr. Nico Boon from Ghent University and Dr. Mohamed Chami from University of Basel, who share a lot of great ideas and give a lot of kind help in our collaboration. Furthermore, I would like to express my sincere thanks to: Hansueli, Thommy, Christoph, Karin, Teresa and Colette for their great technical support. All members in the “Egli group” (Alessandro, Julian, Michael, Frederik, Marius, Karin, Tony, Franzi, Carina, Stefan, Hans Peter, Eva, Margarete, Ines, Teresa, Felix, Beat and Liz) for the very pleasant working atmosphere, and excellent team work. We shared a lot of enjoyable time in the lab, office and outdoors, as well as plenty of Apéros.
    [Show full text]
  • Discovery of Sheath-Forming, Iron-Oxidizing Zetaproteobacteria at Loihi Seamount, Hawaii, USA Emily J
    RESEARCH ARTICLE Hidden in plain sight: discovery of sheath-forming, iron-oxidizing Zetaproteobacteria at Loihi Seamount, Hawaii, USA Emily J. Fleming1, Richard E. Davis2, Sean M. McAllister3,, Clara S. Chan4, Craig L. Moyer3, Bradley M. Tebo2 & David Emerson1 1Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, USA; 2Department of Environmental and Biomolecular Systems, Oregon Health and Science University, Portland, OR, USA; 3Department of Biology, Western Washington University, Bellingham, WA, USA and 4Department of Geological Sciences, University of Delaware, Newark, DE, USA Correspondence: David Emerson, Bigelow Abstract Laboratory for Ocean Sciences, PO Box 380, East Boothbay, ME 04544, USA. Tel.: +1 207 Lithotrophic iron-oxidizing bacteria (FeOB) form microbial mats at focused 315 2567; fax: +1 207 315 2329; flow or diffuse flow vents in deep-sea hydrothermal systems where Fe(II) is a e-mail: [email protected] dominant electron donor. These mats composed of biogenically formed Fe(III)-oxyhydroxides include twisted stalks and tubular sheaths, with sheaths Present address: Sean M. McAllister, typically composing a minor component of bulk mats. The micron diameter Department of Geological Sciences, Fe(III)-oxyhydroxide-containing tubular sheaths bear a strong resemblance to University of Delaware, Newark, DE, USA sheaths formed by the freshwater FeOB, Leptothrix ochracea. We discovered that Received 20 December 2012; revised 28 veil-like surface layers present in iron-mats at the Loihi Seamount were domi- – February 2013; accepted 28 February 2013. nated by sheaths (40 60% of total morphotypes present) compared with deeper Final version published online 15 April 2013. (> 1 cm) mat samples (0–16% sheath). By light microscopy, these sheaths appeared nearly identical to those of L.
    [Show full text]
  • Appendix 1. Validly Published Names, Conserved and Rejected Names, And
    Appendix 1. Validly published names, conserved and rejected names, and taxonomic opinions cited in the International Journal of Systematic and Evolutionary Microbiology since publication of Volume 2 of the Second Edition of the Systematics* JEAN P. EUZÉBY New phyla Alteromonadales Bowman and McMeekin 2005, 2235VP – Valid publication: Validation List no. 106 – Effective publication: Names above the rank of class are not covered by the Rules of Bowman and McMeekin (2005) the Bacteriological Code (1990 Revision), and the names of phyla are not to be regarded as having been validly published. These Anaerolineales Yamada et al. 2006, 1338VP names are listed for completeness. Bdellovibrionales Garrity et al. 2006, 1VP – Valid publication: Lentisphaerae Cho et al. 2004 – Valid publication: Validation List Validation List no. 107 – Effective publication: Garrity et al. no. 98 – Effective publication: J.C. Cho et al. (2004) (2005xxxvi) Proteobacteria Garrity et al. 2005 – Valid publication: Validation Burkholderiales Garrity et al. 2006, 1VP – Valid publication: Vali- List no. 106 – Effective publication: Garrity et al. (2005i) dation List no. 107 – Effective publication: Garrity et al. (2005xxiii) New classes Caldilineales Yamada et al. 2006, 1339VP VP Alphaproteobacteria Garrity et al. 2006, 1 – Valid publication: Campylobacterales Garrity et al. 2006, 1VP – Valid publication: Validation List no. 107 – Effective publication: Garrity et al. Validation List no. 107 – Effective publication: Garrity et al. (2005xv) (2005xxxixi) VP Anaerolineae Yamada et al. 2006, 1336 Cardiobacteriales Garrity et al. 2005, 2235VP – Valid publica- Betaproteobacteria Garrity et al. 2006, 1VP – Valid publication: tion: Validation List no. 106 – Effective publication: Garrity Validation List no. 107 – Effective publication: Garrity et al.
    [Show full text]
  • Anaerobic Degradation of Steroid Hormones by Novel Denitrifying Bacteria
    Anaerobic degradation of steroid hormones by novel denitrifying bacteria Von der Fakultät für Mathematik, Informatik und Naturwissenschaften der Rheinisch- Westfälischen Technischen Hochschule Aachen zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften genehmigte Dissertation vorgelegt von Diplom-Biologe Michael Fahrbach aus Bad Mergentheim (Baden-Württemberg) Berichter: Professor Dr. Juliane Hollender Professor Dr. Andreas Schäffer Tag der mündlichen Prüfung: 12. Dezember 2006 Diese Dissertation ist auf den Internetseiten der Hochschulbibliothek online verfügbar. Table of Contents 1 Introduction.....................................................................................................................1 1.1 General information on steroids ...............................................................................1 1.2 Steroid hormones in the environment.......................................................................2 1.2.1 Natural and anthropogenic sources and deposits ............................................2 1.2.2 Potential impact on the environment ................................................................3 1.2.3 Fate of steroid hormones..................................................................................4 1.3 Microbial degradation of steroid hormones and sterols............................................5 1.3.1 Aerobic degradation..........................................................................................5 1.3.2 Anaerobic degradation......................................................................................7
    [Show full text]
  • Novel Nitrite Reductase Domain Structure Suggests a Chimeric
    Novel nitrite reductase domain structure suggests a chimeric denitrification repertoire in Phylum Chloroflexi Sarah Schwartz1, Lily Momper1, L. Thiberio Rangel1, Cara Magnabosco2, Jan Amend3, and Gregory Fournier1 1Massachusetts Institute of Technology 2ETH Zurich 3University of Southern California June 11, 2021 Abstract Denitrification plays a central role in the global nitrogen cycle, reducing and removing nitrogen from marine and terrestrial ecosystems. The flux of nitrogen species through this pathway has a widespread impact, affecting ecological carrying capacity, agriculture, and climate. Nitrite reductase (Nir) and nitric oxide reductase (NOR) are the two central enzymes in this pathway. Here we present a previously unreported Nir domain architecture in members of Phylum Chloroflexi. Phylogenetic analyses of protein domains within Nir indicate that an ancestral horizontal transfer and fusion event produced this chimeric domain architecture. We also identify an expanded genomic diversity of a rarely reported nitric oxide reductase subtype, eNOR. Together, these results suggest a greater diversity of denitrification enzyme arrangements exist than have been previously reported. RESEARCH PAPER TITLE: Novel nitrite reductase domain structure suggests a chimeric denitrification repertoire in Phylum Chloroflexi SHORT TITLE: Novel Denitrification Architecture in Chloroflexi Sarah L. Schwartz1,2*, Lily M. Momper2,3, L. Thiberio Rangel2, Cara Magnabosco4, Jan P. Amend5,6, and Gregory P. Fournier2 1. Microbiology Graduate Program, Massachusetts Institute of Technology 2. Department of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology 3. Exponent, Inc., Pasadena, CA 4. Department of Earth Sciences, ETH Zurich 5. Department of Earth Sciences, University of Southern California 6. Department of Biological Sciences, University of Southern California *Correspondence: [email protected], +1 (415) 497-1747 SUMMARY Denitrification plays a central role in the global nitrogen cycle, reducing and removing nitrogen from ma- rine and terrestrial ecosystems.
    [Show full text]
  • Molecular Analysis of Bacterial Community Succession During Prolonged Compost Curing
    RESEARCH ARTICLE Molecularanalysis of bacterial community succession during prolonged compost curing Michael Danon1, Ingrid H. Franke-Whittle2, Heribert Insam2, Yona Chen1 & Yitzhak Hadar1 1Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, Israel; and 2Institute for Microbiology, University of Innsbruck, Technikerstraße 25d, A-6020 Innsbruck, Austria Downloaded from https://academic.oup.com/femsec/article/65/1/133/620549 by guest on 30 September 2021 Correspondence: Yitzhak Hadar, Abstract Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food The compost environment consists of complex organic materials that form a and Environmental Quality Sciences, The habitat for a rich and diverse microbial community. The aim of this research was to Hebrew University of Jerusalem, PO Box 12, study the dynamics of microbial communities during the compost-curing phase. Rehovot 76100, Israel. Tel.: 1972 8 9489935; Three different methods based on 16S rRNA gene sequence were applied to fax: 1972 8 9468785; e-mail: monitor changes in the microbial communities: (1) denaturing gradient gel [email protected] electrophoresis of PCR-generated rRNA gene fragments; (2) partial rRNA gene clone libraries; and (3) a microarray of oligonucleotide probes targeting rRNA Received 20 December 2007; revised 11 March gene sequences. All three methods indicated distinctive community shifts during 2008; accepted 10 April 2008. First published online 4 June 2008. curing and the dominant species prevailing during the different curing stages were identified. We found a successional transition of different bacterial phylogenetic DOI:10.1111/j.1574-6941.2008.00506.x groups during compost curing. The Proteobacteria were the most abundant phylum in all cases.
    [Show full text]
  • Diplomarbeit
    DIPLOMARBEIT Stable isotope probing-based ecophysiological analysis of sulphate-reducing microorganisms in an acidic fen angestrebter akademischer Grad Magister der Naturwissenschaften (Mag. rer.nat.) Verfasser: Norbert Bittner Matrikel-Nummer: 0040188 Studienrichtung(lt.Studienblatt): Molekulare Biologie Betreuer: O. Univ. Prof. Dr. Michael Wagner Wien, Jänner 2010 Table of contents 1 Introduction............................................................................................................. 1 1.1 The sulphur cycle .............................................................................................. 1 1.2 Physiology and phylogeny of SRP ................................................................... 2 1.3 Dissimilatory reduction of sulphate .................................................................. 5 1.4 Habitats of SRP................................................................................................. 6 1.5 Wetlands, climate and SRP ............................................................................... 7 1.6 Aim of this study .............................................................................................. 8 2 Material and methods ........................................................................................... 10 2.1 Provided samples ............................................................................................ 10 2.1.1 Sampling site .................................................................................................. 10 2.1.2
    [Show full text]
  • Outline Release 7 7C
    Taxonomic Outline of Bacteria and Archaea, Release 7.7 Taxonomic Outline of the Bacteria and Archaea, Release 7.7 March 6, 2007. Part 4 – The Bacteria: Phylum “Proteobacteria”, Class Betaproteobacteria George M. Garrity, Timothy G. Lilburn, James R. Cole, Scott H. Harrison, Jean Euzéby, and Brian J. Tindall Class Betaproteobacteria VP Garrity et al 2006. N4Lid DOI: 10.1601/nm.16162 Order Burkholderiales VP Garrity et al 2006. N4Lid DOI: 10.1601/nm.1617 Family Burkholderiaceae VP Garrity et al 2006. N4Lid DOI: 10.1601/nm.1618 Genus Burkholderia VP Yabuuchi et al. 1993. GOLD ID: Gi01836. GCAT ID: 001596_GCAT. Sequenced strain: SRMrh-20 is from a non-type strain. Genome sequencing is incomplete. Number of genomes of this species sequenced 2 (GOLD) 1 (NCBI). N4Lid DOI: 10.1601/nm.1619 Burkholderia cepacia VP (Palleroni and Holmes 1981) Yabuuchi et al. 1993. <== Pseudomonas cepacia (basonym). Synonym links through N4Lid: 10.1601/ex.2584. Source of type material recommended for DOE sponsored genome sequencing by the JGI: ATCC 25416. High-quality 16S rRNA sequence S000438917 (RDP), U96927 (Genbank). GOLD ID: Gc00309. GCAT ID: 000301_GCAT. Entrez genome id: 10695. Sequenced strain: ATCC 17760, LMG 6991, NCIMB9086 is from a non-type strain. Genome sequencing is completed. Number of genomes of this species sequenced 1 (GOLD) 1 (NCBI). N4Lid DOI: 10.1601/nm.1620 Pseudomonas cepacia VP (ex Burkholder 1950) Palleroni and Holmes 1981. ==> Burkholderia cepacia (new combination). Synonym links through N4Lid: 10.1601/ex.2584. Source of type material recommended for DOE sponsored genome sequencing by the JGI: ATCC 25416. High- quality 16S rRNA sequence S000438917 (RDP), U96927 (Genbank).
    [Show full text]
  • Ochracea Using Single Cell Genomics, Pyrosequencing and FISH
    What’s New Is Old: Resolving the Identity of Leptothrix ochracea Using Single Cell Genomics, Pyrosequencing and FISH Emily J. Fleming1*, Amy E. Langdon1,2, Manuel Martinez-Garcia1, Ramunas Stepanauskas1, Nicole J. Poulton1, E. Dashiell P. Masland1, David Emerson1 1 Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, Maine, United States of America, 2 Department of Biology, Swarthmore College, Swarthmore, Pennsylvania, United States of America Abstract Leptothrix ochracea is a common inhabitant of freshwater iron seeps and iron-rich wetlands. Its defining characteristic is copious production of extracellular sheaths encrusted with iron oxyhydroxides. Surprisingly, over 90% of these sheaths are empty, hence, what appears to be an abundant population of iron-oxidizing bacteria, consists of relatively few cells. Because L. ochracea has proven difficult to cultivate, its identification is based solely on habitat preference and morphology. We utilized cultivation-independent techniques to resolve this long-standing enigma. By selecting the actively growing edge of a Leptothrix-containing iron mat, a conventional SSU rRNA gene clone library was obtained that had 29 clones (42% of the total library) related to the Leptothrix/Sphaerotilus group (#96% identical to cultured representatives). A pyrotagged library of the V4 hypervariable region constructed from the bulk mat showed that 7.2% of the total sequences also belonged to the Leptothrix/Sphaerotilus group. Sorting of individual L. ochracea sheaths, followed by whole genome amplification (WGA) and PCR identified a SSU rRNA sequence that clustered closely with the putative Leptothrix clones and pyrotags. Using these data, a fluorescence in-situ hybridization (FISH) probe, Lepto175, was designed that bound to ensheathed cells.
    [Show full text]
  • Identification and Characterization of the Proteolytic Flagellin from The
    www.nature.com/scientificreports OPEN Identifcation and characterization of the proteolytic fagellin from the common freshwater bacterium Hylemonella gracilis Ulrich Eckhard1,2*, Constantin Blöchl1, Benjamin G. L. Jenkins3, Michael J. Mansfeld3,5, Christian G. Huber1,4, Andrew C. Doxey3,6* & Hans Brandstetter1,4,6 Flagellins are the protein components of bacterial fagella and assemble in up to 20,000 copies to form extracellular fagellar flaments. An unusual family of fagellins was recently discovered that contains a unique metalloprotease domain within its surface-exposed hypervariable region. To date, these proteolytic fagellins (also termed fagellinolysins) have only been characterized in the Gram-positive organism Clostridium haemolyticum, where fagellinolysin was shown to be proteolytically active and capable of cleaving extracellular protein substrates. The biological function of fagellinolysin and its activity in other organisms, however, remain unclear. Here, using molecular biochemistry and proteomics, we have performed an initial characterization of a novel fagellinolysin identifed from Hylemonella gracilis, a Gram-negative organism originally isolated from pond water. We demonstrate that H. gracilis fagellinolysin (HgrFlaMP) is an active calcium-dependent zinc metallopeptidase and characterize its cleavage specifcity profle using both trypsin and GluC-derived peptide libraries and protein substrates. Based on high-throughput degradomic assays, HgrFlaMP cleaved 784 unique peptides and displayed a cleavage site specifcity similar to fagellinolysin from C. haemolyticum. Additionally, by using a set of six protein substrates, we identifed 206 protein-embedded cleavage sites, further refning the substrate preference of HgrFlaMP, which is dominated by large hydrophobic amino acids in P1′, and small hydrophobic or medium-sized polar residues on the amino-terminal side of the scissile bond.
    [Show full text]
  • S1. the Genomes of the Order Burkholderiales (Taxid:80840) in The
    S1. The genomes of the order Burkholderiales (taxid:80840) in the NCBI Genome List (http://www.ncbi.nlm.nih.gov/genome/browse/) 05-09-2014 N Organism/Name Kingdom Group SubGroup Size (Mb) Chr Plasmids Assemblies 1. Burkholderiales bacterium JGI 0001003-L21 Bacteria Proteobacteria Betaproteobacteria 0,070281 - - 1 2. Comamonadaceae bacterium JGI 0001003-E14 Bacteria Proteobacteria Betaproteobacteria 0,113075 - - 1 3. Candidatus Zinderia insecticola Bacteria Proteobacteria Betaproteobacteria 0,208564 1 - 1 4. Comamonadaceae bacterium JGI 0001013-A16 Bacteria Proteobacteria Betaproteobacteria 0,295955 - - 1 5. Oxalobacteraceae bacterium JGI 0001004-J12 Bacteria Proteobacteria Betaproteobacteria 0,301938 - - 1 6. Oxalobacteraceae bacterium JGI 0001002-K6 Bacteria Proteobacteria Betaproteobacteria 0,368093 - - 1 7. Burkholderiales bacterium JGI 0001003-J08 Bacteria Proteobacteria Betaproteobacteria 0,394907 - - 1 8. Burkholderiales bacterium JGI 0001002-H06 Bacteria Proteobacteria Betaproteobacteria 0,415398 - - 1 9. Pelomonas Bacteria Proteobacteria Betaproteobacteria 0,507844 - - 2 10. Leptothrix ochracea Bacteria Proteobacteria Betaproteobacteria 0,510792 - - 1 11. Oxalobacteraceae bacterium JGI 0001012-C15 Bacteria Proteobacteria Betaproteobacteria 0,640975 - - 1 12. Burkholderiales bacterium JGI 0001003-A5 Bacteria Proteobacteria Betaproteobacteria 0,686115 - - 1 13. Oxalobacteraceae bacterium JGI 001010-B17 Bacteria Proteobacteria Betaproteobacteria 0,825556 - - 1 14. Rhizobacter Bacteria Proteobacteria Betaproteobacteria 1,05799 - -
    [Show full text]
  • 3. Influence of Size, Shape and Flexibility on Bacterial Passage Through Micropore Membrane Filters
    Research Collection Doctoral Thesis "The unseen majority": heterotrophic bacteria in freshwater, more than just small and non-cultivable Author(s): Wang, Yingying Publication Date: 2008 Permanent Link: https://doi.org/10.3929/ethz-a-005701575 Rights / License: In Copyright - Non-Commercial Use Permitted This page was generated automatically upon download from the ETH Zurich Research Collection. For more information please consult the Terms of use. ETH Library Diss. ETH No. 17894 “The unseen majority”: heterotrophic bacteria in freshwater, more than just small and non-cultivable A dissertation submitted to ETH ZÜRICH for the degree of Doctor of Sciences presented by Yingying Wang MPhil, The University of Hong Kong born March 16, 1980 in Tianjin citizen of China accepted on the recommendation of Prof. Dr. Thomas Egli, examiner Dr. Frederik Hammes, co-examiner Prof. Dr. Nico Boon, co-examiner Prof. Dr. Jakob Pernthaler, co-examiner Prof. Dr. Martin Ackermann, co-examiner July, 2008 Acknowledgement I am extremely grateful to Prof. Dr. Thomas Egli for his great supervision, invaluable advice, and providing many academic opportunities for me to widen and enrich my knowledge, vision and experience. I am also full of gratitude to Dr. Frederik Hammes for generously sharing his invaluable research experience, and for numerous inspirational discussions. Their professional and excellent guidance during my doctoral study and academic development are highly appreciated. My special thanks go to Prof. Dr. Nico Boon from Ghent University and Dr. Mohamed Chami from University of Basel, who share a lot of great ideas and give a lot of kind help in our collaboration. Furthermore, I would like to express my sincere thanks to: Hansueli, Thommy, Christoph, Karin, Teresa and Colette for their great technical support.
    [Show full text]