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Chemical Structures of Some Examples of Earlier Characterized Antibiotic and Anticancer Specialized
Supplementary figure S1: Chemical structures of some examples of earlier characterized antibiotic and anticancer specialized metabolites: (A) salinilactam, (B) lactocillin, (C) streptochlorin, (D) abyssomicin C and (E) salinosporamide K. Figure S2. Heat map representing hierarchical classification of the SMGCs detected in all the metagenomes in the dataset. Table S1: The sampling locations of each of the sites in the dataset. Sample Sample Bio-project Site depth accession accession Samples Latitude Longitude Site description (m) number in SRA number in SRA AT0050m01B1-4C1 SRS598124 PRJNA193416 Atlantis II water column 50, 200, Water column AT0200m01C1-4D1 SRS598125 21°36'19.0" 38°12'09.0 700 and above the brine N "E (ATII 50, ATII 200, 1500 pool water layers AT0700m01C1-3D1 SRS598128 ATII 700, ATII 1500) AT1500m01B1-3C1 SRS598129 ATBRUCL SRS1029632 PRJNA193416 Atlantis II brine 21°36'19.0" 38°12'09.0 1996– Brine pool water ATBRLCL1-3 SRS1029579 (ATII UCL, ATII INF, N "E 2025 layers ATII LCL) ATBRINP SRS481323 PRJNA219363 ATIID-1a SRS1120041 PRJNA299097 ATIID-1b SRS1120130 ATIID-2 SRS1120133 2168 + Sea sediments Atlantis II - sediments 21°36'19.0" 38°12'09.0 ~3.5 core underlying ATII ATIID-3 SRS1120134 (ATII SDM) N "E length brine pool ATIID-4 SRS1120135 ATIID-5 SRS1120142 ATIID-6 SRS1120143 Discovery Deep brine DDBRINP SRS481325 PRJNA219363 21°17'11.0" 38°17'14.0 2026– Brine pool water N "E 2042 layers (DD INF, DD BR) DDBRINE DD-1 SRS1120158 PRJNA299097 DD-2 SRS1120203 DD-3 SRS1120205 Discovery Deep 2180 + Sea sediments sediments 21°17'11.0" -
The Role of Earthworm Gut-Associated Microorganisms in the Fate of Prions in Soil
THE ROLE OF EARTHWORM GUT-ASSOCIATED MICROORGANISMS IN THE FATE OF PRIONS IN SOIL Von der Fakultät für Lebenswissenschaften der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) genehmigte D i s s e r t a t i o n von Taras Jur’evič Nechitaylo aus Krasnodar, Russland 2 Acknowledgement I would like to thank Prof. Dr. Kenneth N. Timmis for his guidance in the work and help. I thank Peter N. Golyshin for patience and strong support on this way. Many thanks to my other colleagues, which also taught me and made the life in the lab and studies easy: Manuel Ferrer, Alex Neef, Angelika Arnscheidt, Olga Golyshina, Tanja Chernikova, Christoph Gertler, Agnes Waliczek, Britta Scheithauer, Julia Sabirova, Oleg Kotsurbenko, and other wonderful labmates. I am also grateful to Michail Yakimov and Vitor Martins dos Santos for useful discussions and suggestions. I am very obliged to my family: my parents and my brother, my parents on low and of course to my wife, which made all of their best to support me. 3 Summary.....................................................………………………………………………... 5 1. Introduction...........................................................................................................……... 7 Prion diseases: early hypotheses...………...………………..........…......…......……….. 7 The basics of the prion concept………………………………………………….……... 8 Putative prion dissemination pathways………………………………………….……... 10 Earthworms: a putative factor of the dissemination of TSE infectivity in soil?.………. 11 Objectives of the study…………………………………………………………………. 16 2. Materials and Methods.............................…......................................................……….. 17 2.1 Sampling and general experimental design..................................................………. 17 2.2 Fluorescence in situ Hybridization (FISH)………..……………………….………. 18 2.2.1 FISH with soil, intestine, and casts samples…………………………….……... 18 Isolation of cells from environmental samples…………………………….………. -
The 2014 Golden Gate National Parks Bioblitz - Data Management and the Event Species List Achieving a Quality Dataset from a Large Scale Event
National Park Service U.S. Department of the Interior Natural Resource Stewardship and Science The 2014 Golden Gate National Parks BioBlitz - Data Management and the Event Species List Achieving a Quality Dataset from a Large Scale Event Natural Resource Report NPS/GOGA/NRR—2016/1147 ON THIS PAGE Photograph of BioBlitz participants conducting data entry into iNaturalist. Photograph courtesy of the National Park Service. ON THE COVER Photograph of BioBlitz participants collecting aquatic species data in the Presidio of San Francisco. Photograph courtesy of National Park Service. The 2014 Golden Gate National Parks BioBlitz - Data Management and the Event Species List Achieving a Quality Dataset from a Large Scale Event Natural Resource Report NPS/GOGA/NRR—2016/1147 Elizabeth Edson1, Michelle O’Herron1, Alison Forrestel2, Daniel George3 1Golden Gate Parks Conservancy Building 201 Fort Mason San Francisco, CA 94129 2National Park Service. Golden Gate National Recreation Area Fort Cronkhite, Bldg. 1061 Sausalito, CA 94965 3National Park Service. San Francisco Bay Area Network Inventory & Monitoring Program Manager Fort Cronkhite, Bldg. 1063 Sausalito, CA 94965 March 2016 U.S. Department of the Interior National Park Service Natural Resource Stewardship and Science Fort Collins, Colorado The National Park Service, Natural Resource Stewardship and Science office in Fort Collins, Colorado, publishes a range of reports that address natural resource topics. These reports are of interest and applicability to a broad audience in the National Park Service and others in natural resource management, including scientists, conservation and environmental constituencies, and the public. The Natural Resource Report Series is used to disseminate comprehensive information and analysis about natural resources and related topics concerning lands managed by the National Park Service. -
Autotrophy in Groundwater Ecosystems
Dissertation der Fakultät für Biologie der Ludwig-Maximilians-Universität München Autotrophy in Groundwater Ecosystems Dissertation zur Erlangung des naturwissenschaftlichen Doktorgrades vorgelegt von Claudia Sabine Kellermann aus München München im November 2008 1. Gutachter: Prof. Dr. Anton Hartmann, LMU München 2. Gutachter: Prof. Dr. Dirk Schüler, LMU München Tag der Abgabe: 06.11.2008 Tag des Promotionskolloquiums: 15.07.2009 Publications originating from this Thesis Chapter 2 Kellermann, C & Griebler, C (2008) Thiobacillus thiophilus D24TNT sp. nov., a chemolithoautotrophic, thiosulfate-oxidizing bacterium isolated from contaminated aquifer sediments. International Journal of Systematic and Evolutionary Microbiology (IJSEM), 59: 583-588 Chapter 3 Kellermann, C, Selesi, D, Hartmann, A, Lee, N, Hügler, M, Esperschütz, J, & Griebler, C (2008) Chemolithoautotrophy in an organically polluted aquifer – Potential for CO2 fixation and in situ bacterial autotrophic activity. (in preparation) Contributions Chapter 3 Enzyme assays were performed in cooperation with Dr. Michael Hügler at the IFM- GEOMAR, Kiel, Germany. Chapter 4 FISH-MAR analysis was performed in cooperation with Prof. Dr. Natuschka Lee at the Technical University Munich, Germany. Enzyme assays were performed in cooperation with Dr. Michael Hügler at the IFM-GEOMAR, Kiel, Germany. PLFA analysis was performed by Dr. Jürgen Esperschütz at the Institute of Soil Ecology, Helmholtz Center Munich, Germany. I hereby confirm the above statements Claudia Kellermann Prof. Dr. Anton Hartmann Autotrophy in Groundwater Ecosystems Claudia Kellermann Abstract: The major role in global net CO2 fixation plays photosynthesis of green plants, algae and cyanobacteria, but other microorganisms are also important concerning autotrophy; i.e. autotrophic microorganisms can be found in most bacterial groups (Eubacteria) and there are even numerous representatives within the Archaea. -
Identification of Genes Responsible for Ochratoxin-A Biodegradation By
Szent István University PhD School of Environmental Sciences Identification of genes responsible for ochratoxin-A biodegradation by Cupriavidus basilensis ŐR16 and valuation of Cupriavidus genus for mycotoxins biodegradation potential Thesis of Doctoral Dissertation (PhD) Mohammed Talib Jasim AL-Nussairawi Gödöllő, Hungary 2020 I. DECLARATION I declare that this thesis is a record of original work and contains no material that has been accepted for the award of any other degree or diploma in any university. To the best of my knowledge and belief, this thesis contains no material previously published or written by another person, except where due reference is made in the text. Name: Ph.D. School of Environmental Sciences Discipline: Environmental Sciences / Biotechnology Leader of Doctoral School: Csákiné Dr. Erika Michéli, Ph.D. professor, head of department Department of Soil science and Agrochemistry Faculty of Agricultural and Environmental Sciences Institute of Environmental Sciences Szent István University Supervisor: Matyas Cserhati, Ph.D. associate professor Department of Environmental Protection and Ecotoxicology Faculty of Agricultural and Environmental Sciences Institute of Aquaculture and Environmental Protection Szent István University ........................................................... ................................................... Approval of School Leader Approval of Supervisor 2 Table of Contents I. DECLARATION ................................................................................................................... -
Comamonas: Relationship to Aquaspirillum Aquaticum, E
INTERNATIONALJOURNAL OF SYSTEMATICBACTERIOLOGY, July 1991, p. 427-444 Vol. 41, No. 3 0020-7713/91/030427- 18$02 .OO/O Copyright 0 1991, International Union of Microbiological Societies Polyphasic Taxonomic Study of the Emended Genus Comamonas: Relationship to Aquaspirillum aquaticum, E. Falsen Group 10, and Other Clinical Isolates A. WILLEMS,l B. POT,l E. FALSEN,2 P. VANDAMME,' M. GILLIS,l* K. KERSTERS,l AND J. DE LEY' Laboratorium voor Microbiologie en Microbiele Genetica, Rijksuniversiteit, B-9000 Ghent, Belgium, and Culture Collection, Department of Clinical Bacteriology, University of Goteborg, S-413 46 Goteborg, Sweden2 We used DNA-rRNA hybridization, DNA base composition, polyacrylamide gel electrophoresis of whole-cell proteins, DNA-DNA hybridization, numerical analysis of phenotypic features, and immunotyping to study the taxonomy of the genus Comamonas. The relationships of this genus to Aquaspirillum aquaticum and a group of clinical isolates (E. Falsen group 10 [EF lo]) were studied. Our DNA and rRNA hybridization results indicate that the genus Comamonas consists of at least the following five genotypic groups: (i) Comamonas acidovoruns, (ii) Comamonas fesfosferoni,(iii) Comamonas ferrigena, (iv) A. aquaticum and a number of EF 10 strains, and (v) other EF 10 strains, several unnamed clinical isolates, and some misnamed strains of Pseudomonas alcaligenes and Pseudomonas pseudoalcaligenes subsp. pseudoalcaligenes. The existence of these five groups was confirmed by the results of immunotyping and protein gel electrophoresis. A numerical analysis of morpho- logical, auxanographic, and biochemical data for the same organisms revealed the existence of three large phena. Two of these phena (C. acidovorans and C. tesfosferoni)correspond to two of the genotypic groups. -
Additional File 1
Additional file 1 Microbial succession during the transition from active to inactive stages of deep-sea hydrothermal vent sulfide chimneys Jialin Hou, Stefan M. Sievert, Yinzhao Wang, Jeff S. Seewald, Vengadesh Perumal Natarajan Fengping Wang*, Xiang Xiao* a b 100 100 75 75 Taxa Alphaproteobacteria Taxa Betaproteobacteria Aquificae Deinococcus-Thermus Campylobacteria Deltaproteobacteria 50 Euryarchaeata 50 Euryarchaeata Others Gammaproteobacteria Percentage Percentage Thermodesulfobacterium Muproteobacteria Unclassified Nitrospirae Others Unclassified 25 25 0 0 aclA aclB cdhA cdhC napA napB norB nosZ sat soxA soxC soxY soxZ sqr aprA aprB dsrA dsrB narG nirB PRK rbcL rbcS Gene Gene Figure S1 Taxonomic classification of key functional genes retrieved from the L- and M-vent chimeny.(a) The key genes enriched in the active L-vent chimney. (b) The key genes enriched in the recently inactive M-vent chimney. Reductive bacterial type Reductive archaeal type Chlorobi group Archaeoglobus(3) Oxidative bacterial type Crenarchaeota Alphaproteobacteria(1/16) Nitrospirae(12) Acidobacteria(1/14) Betaproteobacteria Firmicutes groups Gammaproteobacteria (20) Tree scale: 1 Deltaproteobacteria(9/10) Reductive bacterial type Figure S2 Maximum-likelihood phylogeny of dsrA genes retrieved from L- and M-vent chimney. The red branches represent the dsrA genes recovered from active the L-vent chimney, while the blue ones are those from recently inactive M-vent chimney. Numbers of dsrA gene for each sample are displayed in the parenthesis after the clade name. -
國立高雄海洋科技大學 National Kaohsiung Marine University
國立高雄海洋科技大學 NATIONAL KAOHSIUNG MARINE UNIVERSITY 專任教師著作目錄 2005~2008 序 本校創立於民國 35 年,歷經水產職業學校、海事專科學校、 海洋技術學院等學制的變革,至民國 93 年,始改名為國立高雄海 洋科技大學,成為一所以發展海洋科技教育為主軸的高等科技學 府。回顧這 62 年來,本校一直肩負著為國家培育海洋專業人才, 發展海洋應用科技的重責大任,也見證臺灣在這段期間,於教育、 經濟等方面發展成長的軌跡。 截至 97 學年度第 1 學期為止,本校專任教師(含校長)計 231 名,分屬於海事學院、管理學院、海洋工程學院與水圈學院等四 個學院,以及教導全校學生基礎教育、通識教育的共同教育委員 會。全體教師彼此尊重,各司其職,各盡其分的承擔研究、教學 及服務等工作,推動校務不斷的向上發展。 自 94 學年度起,為彰顯改名科技大學後的績效,鼓勵專任教 師將其研究成果由本校研發處開始逐年編印《專任教師著作目 錄》,用以彙整教師在論文、專利、技術報告及專著等方面的成果。 期待本目錄的編印可以達到下列的效益:一、呈現本校教師辛苦 耕耘的果實及本校研究發展的特色;二、提供海洋科技領域相關 人員最新的資訊;三、帶動本校教師相互切磋琢磨的研究風氣。 自本目錄發行以來,本校的研究風氣已明顯增長,研究的能 量正不斷的累積。從前三年出版的目錄看出,除創刊初期,以海 洋教育為主軸的學科仍維持優異的研究成果之外,隨著海洋相關 系科的增設,研究所的陸續成立,近年來,本校在管理、工程、 電子、生物科技及共同教育等各領域的研究,與產學合作的推動, 成果也相當豐碩。 學術研究的精神是創新,學術研究的核心價值是不斷進步,學 術研究風氣的形成則有賴全體教師共同的經營。在 97 學年度《專任 教師著作目錄》出版前夕,本人衷心期盼全體教師,為了提升教學 品質,促進產學合作,不分專業教師或共同教育的教師,大家都 能在教學、服務之餘,竭盡所能的從事學術研究。因為研究是教 學的基礎,也是促進產學合作的活水源頭。 本目錄資料的編纂力求確實,雖經研發處同仁校對再三,惟 恐仍有疏漏誤植的現象,請不吝賜教指正,不勝感激。是為序。 校長 2008 年 12 月 5 日於國立高雄海洋科技大學 97 專任教師名冊 國立高雄海洋科技大學專任教師名冊(97.11.25) 科 系 職 稱 姓 名 科 系 職 稱 姓 名 校長 校長 周照仁 輪機工程系 副教授 蘇俊連 航運技術系 副教授 廖宗 輪機工程系 助理教授 黃耀新 航運技術系 副教授 林富振 輪機工程系 助理教授 楊政達 航運技術系 副教授 郭福村 輪機工程系 助理教授 蕭海明 航運技術系 副教授 陳希敬 輪機工程系 助理教授 吳俊文 航運技術系 副教授 王一三 輪機工程系 講師 郭振亞 航運技術系 副教授 周建張 輪機工程系 講師 楊子傑 航運技術系 副教授 胡家聲 輪機工程系 講師 鍾振弘 航運技術系 副教授 陳彥宏 輪機工程系 講師 邱時甫 航運技術系 助理教授 蘇東濤 輪機工程系 助教 王水音 航運技術系 助理教授 黃振邦 航運管理系 副教授 戴輝煌 航運技術系 講師 苟榮華 航運管理系 副教授 許文楷 航運技術系 講師 俞惠麟 航運管理系 副教授 于惠蓉 航運技術系 講師 洪秋明 航運管理系 副教授 楊鈺池 航運技術系 講師 陳崑旭 航運管理系 副教授 孫智嫻 航運技術系 講師 謝坤山 航運管理系 副教授 曾文瑞 航運技術系 講師 劉安白 航運管理系 助理教授 趙清成 航運技術系 講師 文展權 航運管理系 講師 連淑君 航運技術系 講師級專業技術人員 蔣克雄 航運管理系 講師 蔣文玉 輪機工程系 教授 張始偉 -