國立高雄海洋科技大學 National Kaohsiung Marine University
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Rational Construction of Genome-Reduced Burkholderiales Chassis Facilitates Efficient Heterologous Production of Natural Products from Proteobacteria
ARTICLE https://doi.org/10.1038/s41467-021-24645-0 OPEN Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria Jiaqi Liu1, Haibo Zhou1, Zhiyu Yang1, Xue Wang1, Hanna Chen1, Lin Zhong1, Wentao Zheng1, Weijing Niu1, Sen Wang2, Xiangmei Ren2, Guannan Zhong1, Yan Wang3, Xiaoming Ding4, Rolf Müller 5, Youming Zhang1 & ✉ Xiaoying Bian 1 1234567890():,; Heterologous expression of biosynthetic gene clusters (BGCs) avails yield improvements and mining of natural products, but it is limited by lacking of more efficient Gram-negative chassis. The proteobacterium Schlegelella brevitalea DSM 7029 exhibits potential for het- erologous BGC expression, but its cells undergo early autolysis, hindering further applica- tions. Herein, we rationally construct DC and DT series genome-reduced S. brevitalea mutants by sequential deletions of endogenous BGCs and the nonessential genomic regions, respectively. The DC5 to DC7 mutants affect growth, while the DT series mutants show improved growth characteristics with alleviated cell autolysis. The yield improvements of six proteobacterial natural products and successful identification of chitinimides from Chit- inimonas koreensis via heterologous expression in DT mutants demonstrate their superiority to wild-type DSM 7029 and two commonly used Gram-negative chassis Escherichia coli and Pseudomonas putida. Our study expands the panel of Gram-negative chassis and facilitates the discovery of natural products by heterologous expression. 1 Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, China. 2 Core Facilities for Life and Environmental Sciences, Shandong University, Qingdao, Shandong, China. 3 College of Marine Life Sciences, and Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, China. -
Genus-Wide Comparison of Pseudovibrio Bacterial Genomes Reveal Diverse Adaptations to Different Marine Invertebrate Hosts
RESEARCH ARTICLE Genus-wide comparison of Pseudovibrio bacterial genomes reveal diverse adaptations to different marine invertebrate hosts Anoop Alex1,2*, Agostinho Antunes1,2* 1 CIIMAR/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Porto, Portugal, 2 Department of Biology, Faculty of Sciences, University of Porto, Porto, Portugal * [email protected] (AA); [email protected] (AA) a1111111111 a1111111111 a1111111111 a1111111111 Abstract a1111111111 Bacteria belonging to the genus Pseudovibrio have been frequently found in association with a wide variety of marine eukaryotic invertebrate hosts, indicative of their versatile and symbiotic lifestyle. A recent comparison of the sponge-associated Pseudovibrio genomes has shed light on the mechanisms influencing a successful symbiotic association with OPEN ACCESS sponges. In contrast, the genomic architecture of Pseudovibrio bacteria associated with Citation: Alex A, Antunes A (2018) Genus-wide other marine hosts has received less attention. Here, we performed genus-wide compara- comparison of Pseudovibrio bacterial genomes reveal diverse adaptations to different marine tive analyses of 18 Pseudovibrio isolated from sponges, coral, tunicates, flatworm, and sea- invertebrate hosts. PLoS ONE 13(5): e0194368. water. The analyses revealed a certain degree of commonality among the majority of https://doi.org/10.1371/journal.pone.0194368 sponge- and coral-associated bacteria. Isolates from other marine invertebrate host, tuni- Editor: Zhi Ruan, Zhejiang University, CHINA cates, exhibited a genetic repertoire for cold adaptation and specific metabolic abilities Received: November 12, 2017 including mucin degradation in the Antarctic tunicate-associated bacterium Pseudovibrio sp. Tun.PHSC04_5.I4. Reductive genome evolution was simultaneously detected in the flat- Accepted: March 1, 2018 worm-associated bacteria and the sponge-associated bacterium P. -
Introduction to Cell-Cell Communication
Intercellular Bacterial Signalling in Activated Sludge by Grace Chong A thesis submitted in fulfilment of the requirements for the degree of Doctor of Philosophy School of Biotechnology and Biomolecular Sciences Faculty of Science The University of New South Wales Sydney, Australia March 2010 Table of Contents Table of Contents ........................................................................................................ 2 Acknowledgements ...................................................................................................... 7 Abstract ........................................................................................................................ 9 Originality Statement................................................................................................ 11 List of Figures ............................................................................................................ 12 List of Tables ............................................................................................................. 14 List of Abbreviations ................................................................................................ 15 1 General introduction and literature review ...................................................... 18 1.1 Introduction ........................................................................................... 18 1.2 Introduction to cell-cell communication ............................................... 18 1.2.1 Quorum sensing paradigm ............................................................... -
Meanders As a Scaling Motif for Understanding of Floodplain Soil Microbiome and Biogeochemical Potential at the Watershed Scale
Supplementary Information for: Meanders as a scaling motif for understanding of floodplain soil microbiome and biogeochemical potential at the watershed scale Paula B. Matheus Carnevali1, Adi Lavy1, Alex D. Thomas2, Alexander Crits-Christoph3, Spencer Diamond1, Raphaeël Meéheust1,4, Matthew R. Olm3,^, Allison Sharrar1, Shufei Lei1, WenminG Dong5, Nicola Falco5, Nicholas Bouskill5, Michelle Newcomer5, Peter Nico5, Haruko Wainwright5, Dipankar Dwivedi5, Kenneth H. Williams5, Susan Hubbard5, Jillian F. Banfield1,2,3,4,5,6,*. 1Department of Earth and Planetary Science, University of California, Berkeley, CA, USA. 2Department of Environmental Science, Policy, and Management, University of California, Berkeley, CA, USA. 3Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA. 4Innovative Genomics Institute, Berkley, CA, USA. 5Earth and Environmental Sciences Area, Lawrence Berkeley National Laboratory, Berkeley, CA, USA 6Chan Zuckerberg Biohub, San Francisco, CA, USA. Current affiliation: ^ Department of Microbiology and Immunology, Stanford University, Palo Alto, CA, USA *Corresponding author: [email protected] File contents: Supplementary figures 1-7 List of supplementary tables 1-14 List of supplementary data 1-8 Supplementary Figure 1. Percent of samples within each floodplain where a genome was detected at the sub-species level (98% ANI). Presence or absence was determined based on Hellinger transformed abundance (average coverage ³ 0.01). (a) Detection regardless of where the genome was reconstructed -
Horizontal Gene Transfer in the Sponge Amphimedon Queenslandica
Horizontal gene transfer in the sponge Amphimedon queenslandica Simone Summer Higgie BEnvSc (Honours) A thesis submitted for the degree of Doctor of Philosophy at The University of Queensland in 2018 School of Biological Sciences Abstract Horizontal gene transfer (HGT) is the nonsexual transfer of genetic sequence across species boundaries. Historically, HGT has been assumed largely irrelevant to animal evolution, though widely recognised as an important evolutionary force in bacteria. From the recent boom in whole genome sequencing, many cases have emerged strongly supporting the occurrence of HGT in a wide range of animals. However, the extent, nature and mechanisms of HGT in animals remain poorly understood. Here, I explore these uncertainties using 576 HGTs previously reported in the genome of the demosponge Amphimedon queenslandica. The HGTs derive from bacterial, plant and fungal sources, contain a broad range of domain types, and many are differentially expressed throughout development. Some domains are highly enriched; phylogenetic analyses of the two largest groups, the Aspzincin_M35 and the PNP_UDP_1 domain groups, suggest that each results from one or few transfer events followed by post-transfer duplication. Their differential expression through development, and the conservation of domains and duplicates, together suggest that many of the HGT-derived genes are functioning in A. queenslandica. The largest group consists of aspzincins, a metallopeptidase found in bacteria and fungi, but not typically in animals. I detected aspzincins in representatives of all four of the sponge classes, suggesting that the original sponge aspzincin was transferred after sponges diverged from their last common ancestor with the Eumetazoa, but before the contemporary sponge classes emerged. -
Microbial Degradation of Organic Micropollutants in Hyporheic Zone Sediments
Microbial degradation of organic micropollutants in hyporheic zone sediments Dissertation To obtain the Academic Degree Doctor rerum naturalium (Dr. rer. nat.) Submitted to the Faculty of Biology, Chemistry, and Geosciences of the University of Bayreuth by Cyrus Rutere Bayreuth, May 2020 This doctoral thesis was prepared at the Department of Ecological Microbiology – University of Bayreuth and AG Horn – Institute of Microbiology, Leibniz University Hannover, from August 2015 until April 2020, and was supervised by Prof. Dr. Marcus. A. Horn. This is a full reprint of the dissertation submitted to obtain the academic degree of Doctor of Natural Sciences (Dr. rer. nat.) and approved by the Faculty of Biology, Chemistry, and Geosciences of the University of Bayreuth. Date of submission: 11. May 2020 Date of defense: 23. July 2020 Acting dean: Prof. Dr. Matthias Breuning Doctoral committee: Prof. Dr. Marcus. A. Horn (reviewer) Prof. Harold L. Drake, PhD (reviewer) Prof. Dr. Gerhard Rambold (chairman) Prof. Dr. Stefan Peiffer In the battle between the stream and the rock, the stream always wins, not through strength but by perseverance. Harriett Jackson Brown Jr. CONTENTS CONTENTS CONTENTS ............................................................................................................................ i FIGURES.............................................................................................................................. vi TABLES .............................................................................................................................. -
Templat Tesis Dan Disertasi
BACTERIAL DIVERSITY AND LIPASE PRODUCING BACTERIA IN FOREST AND OIL PALM PLANTATION AT SAROLANGUN JAMBI INDONESIA MARINI WIJAYANTI GRADUATE SCHOOL BOGOR AGRICULTURAL UNIVERSITY BOGOR 2015 DECLARATION OF THE SOURCE OF THIS DISSERTATION I declare that this dissertation, entitled Bacterial Diversity and Lipase Producing Bacteria in Forest and Oil Palm Plantation at Sarolangun Jambi Indonesia is entirely my own work, assisted by a supervisory committee and has not been submitted in any form for another degree or diploma to any university or other tertiary institution of education. Where this dissertation draws on existing publications, those sources are cited in the text and listed in the references section. Bogor, August 2015 Marini Wijayanti G361110011 RINGKASAN MARINI WIJAYANTI. Keragaman Bakteri dan Bakteri Penghasil Lipase dalam Hutan dan Perkebunan Sawit di Sarolangun Jambi Indonesia. Dibimbing oleh ANJA MERYANDINI, ARIS TRI WAHYUDI, and MUNTI YUHANA. Deforestasi untuk pembukaan lahan perkebunan sawit paling masif terjadi di Asia Tenggara. Hal ini berdampak negatif pada keragaman hayati dan lingkungan. Tanah dan sedimen pada penelitian ini diperoleh dari lapisan atas tanah hutan dataran rendah dan perkebunan sawit, serta perairan yang disekitar situs tersebut. Hutan tersebut berlokasi di Taman Nasional Bukit Duabelas, dan perkebunan sawit sekitar hutan di Sarolangun Jambi Indonesia. Keragaman komunitas bakteri dari tanah dan sedimen perairan area hutan dan kebun sawit Sumatra dipelajari menggunakan pyrosequencing gen 16S rRNA dan indeks keragaman umumnya. Pendekatan filogenetik digunakan untuk mengungkap perubahan komunitas filotipe bakteri dan genusnya di kedua area. Pendekatan ekologis menggunakan nilai pH, kandungan Karbon (C) total, Nitrogen (N) total, Fosfor (P) tersedia dan keragaman bakteri menggunakan indeks Shannon dan Simpson, dan kelimpahan bakteri dengan indeks Chao1-ACE dan OTUs. -
Appendix 1. Validly Published Names, Conserved and Rejected Names, And
Appendix 1. Validly published names, conserved and rejected names, and taxonomic opinions cited in the International Journal of Systematic and Evolutionary Microbiology since publication of Volume 2 of the Second Edition of the Systematics* JEAN P. EUZÉBY New phyla Alteromonadales Bowman and McMeekin 2005, 2235VP – Valid publication: Validation List no. 106 – Effective publication: Names above the rank of class are not covered by the Rules of Bowman and McMeekin (2005) the Bacteriological Code (1990 Revision), and the names of phyla are not to be regarded as having been validly published. These Anaerolineales Yamada et al. 2006, 1338VP names are listed for completeness. Bdellovibrionales Garrity et al. 2006, 1VP – Valid publication: Lentisphaerae Cho et al. 2004 – Valid publication: Validation List Validation List no. 107 – Effective publication: Garrity et al. no. 98 – Effective publication: J.C. Cho et al. (2004) (2005xxxvi) Proteobacteria Garrity et al. 2005 – Valid publication: Validation Burkholderiales Garrity et al. 2006, 1VP – Valid publication: Vali- List no. 106 – Effective publication: Garrity et al. (2005i) dation List no. 107 – Effective publication: Garrity et al. (2005xxiii) New classes Caldilineales Yamada et al. 2006, 1339VP VP Alphaproteobacteria Garrity et al. 2006, 1 – Valid publication: Campylobacterales Garrity et al. 2006, 1VP – Valid publication: Validation List no. 107 – Effective publication: Garrity et al. Validation List no. 107 – Effective publication: Garrity et al. (2005xv) (2005xxxixi) VP Anaerolineae Yamada et al. 2006, 1336 Cardiobacteriales Garrity et al. 2005, 2235VP – Valid publica- Betaproteobacteria Garrity et al. 2006, 1VP – Valid publication: tion: Validation List no. 106 – Effective publication: Garrity Validation List no. 107 – Effective publication: Garrity et al. -
Contents Topic 1. Introduction to Microbiology. the Subject and Tasks
Contents Topic 1. Introduction to microbiology. The subject and tasks of microbiology. A short historical essay………………………………………………………………5 Topic 2. Systematics and nomenclature of microorganisms……………………. 10 Topic 3. General characteristics of prokaryotic cells. Gram’s method ………...45 Topic 4. Principles of health protection and safety rules in the microbiological laboratory. Design, equipment, and working regimen of a microbiological laboratory………………………………………………………………………….162 Topic 5. Physiology of bacteria, fungi, viruses, mycoplasmas, rickettsia……...185 TOPIC 1. INTRODUCTION TO MICROBIOLOGY. THE SUBJECT AND TASKS OF MICROBIOLOGY. A SHORT HISTORICAL ESSAY. Contents 1. Subject, tasks and achievements of modern microbiology. 2. The role of microorganisms in human life. 3. Differentiation of microbiology in the industry. 4. Communication of microbiology with other sciences. 5. Periods in the development of microbiology. 6. The contribution of domestic scientists in the development of microbiology. 7. The value of microbiology in the system of training veterinarians. 8. Methods of studying microorganisms. Microbiology is a science, which study most shallow living creatures - microorganisms. Before inventing of microscope humanity was in dark about their existence. But during the centuries people could make use of processes vital activity of microbes for its needs. They could prepare a koumiss, alcohol, wine, vinegar, bread, and other products. During many centuries the nature of fermentations remained incomprehensible. Microbiology learns morphology, physiology, genetics and microorganisms systematization, their ecology and the other life forms. Specific Classes of Microorganisms Algae Protozoa Fungi (yeasts and molds) Bacteria Rickettsiae Viruses Prions The Microorganisms are extraordinarily widely spread in nature. They literally ubiquitous forward us from birth to our death. Daily, hourly we eat up thousands and thousands of microbes together with air, water, food. -
Biodiversidad Bacteriana Marina: Nuevos Taxones Cultivables
Departamento de Microbiología y Ecología Colección Española de Cultivos Tipo Doctorado en Biotecnología Biodiversidad bacteriana marina: nuevos taxones cultivables Directores de Tesis David Ruiz Arahal Mª Jesús Pujalte Domarco Mª Carmen Macián Rovira Teresa Lucena Reyes Tesis Doctoral Valencia, 2012 Dr. David Ruiz Arahal , Profesor Titular del Departamento de Microbiología y Ecología de la Universidad de Valencia, Dra. María Jesús Pujalte Domarco , Catedrática del Departamento de Microbiología y Ecología de la Universidad de Valencia, y Dra. Mª Carmen Macián Rovira , Técnico Superior de Investigación de la Colección Española de Cultivos Tipo de la Universidad de Valencia, CERTIFICAN: Que Teresa Lucena Reyes, Licenciada en Ciencias Biológicas por la Universidad de Valencia, ha realizado bajo su dirección el trabajo titulado “Biodiversidad bacteriana marina: nuevos taxones cultivables”, que presenta para optar al grado de Doctor en Ciencias Biológicas por la Universidad de Valencia. Y para que conste, en el cumplimiento de la legislación vigente, firman el presente certificado en Valencia, a. David Ruiz Arahal Mª Jesús Pujalte Domarco Mª Carmen Macián Rovira Relación de publicaciones derivadas de la presente Tesis Doctoral Lucena T , Pascual J, Garay E, Arahal DR, Macián MC, Pujalte MJ (2010) . Haliea mediterranea sp. nov., a marine gammaproteobacterium. Int J Syst Evol Microbiol 60 , 1844-8. Lucena T, Pascual J, Giordano A, Gambacorta A, Garay E, Arahal DR, Macián MC, Pujalte MJ (2010) . Euzebyella saccharophila gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae . Int J Syst Evol Microbiol 60 , 2871-6. Lucena T, Ruvira MA, Pascual J, Garay E, Macián MC, Arahal DR, Pujalte MJ (2011) . Photobacterium aphoticum sp. -
Phylogenomic Analyses of Bradyrhizobium Reveal Uneven Distribution of the Lateral and Subpolar Flagellar Systems, Which Extends to Rhizobiales
microorganisms Article Phylogenomic Analyses of Bradyrhizobium Reveal Uneven Distribution of the Lateral and Subpolar Flagellar Systems, Which Extends to Rhizobiales Daniel Garrido-Sanz 1 , Miguel Redondo-Nieto 1, Elías Mongiardini 2, Esther Blanco-Romero 1, David Durán 1, Juan I. Quelas 2, Marta Martin 1, Rafael Rivilla 1 , Aníbal R. Lodeiro 2 and M. Julia Althabegoiti 2,* 1 Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, c/Darwin 2, 28049 Madrid, Spain; [email protected] (D.G.-S.); [email protected] (M.R.-N.); [email protected] (E.B.-R.); [email protected] (D.D.); [email protected] (M.M.); [email protected] (R.R.) 2 Instituto de Biotecnología y Biología Molecular (IBBM), Facultad de Ciencias Exactas, UNLP y CCT-La Plata-CONICET, La Plata B1900, Argentina; [email protected] (E.M.); [email protected] (J.I.Q.); [email protected] (A.R.L.) * Correspondence: [email protected] Received: 19 December 2018; Accepted: 11 February 2019; Published: 13 February 2019 Abstract: Dual flagellar systems have been described in several bacterial genera, but the extent of their prevalence has not been fully explored. Bradyrhizobium diazoefficiens USDA 110T possesses two flagellar systems, the subpolar and the lateral flagella. The lateral flagellum of Bradyrhizobium displays no obvious role, since its performance is explained by cooperation with the subpolar flagellum. In contrast, the lateral flagellum is the only type of flagella present in the related Rhizobiaceae family. In this work, we have analyzed the phylogeny of the Bradyrhizobium genus by means of Genome-to-Genome Blast Distance Phylogeny (GBDP) and Average Nucleotide Identity (ANI) comparisons of 128 genomes and divided it into 13 phylogenomic groups. -
Metabolic Roles of Uncultivated Bacterioplankton Lineages in the Northern Gulf of Mexico 2 “Dead Zone” 3 4 J
bioRxiv preprint doi: https://doi.org/10.1101/095471; this version posted June 12, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 1 Metabolic roles of uncultivated bacterioplankton lineages in the northern Gulf of Mexico 2 “Dead Zone” 3 4 J. Cameron Thrash1*, Kiley W. Seitz2, Brett J. Baker2*, Ben Temperton3, Lauren E. Gillies4, 5 Nancy N. Rabalais5,6, Bernard Henrissat7,8,9, and Olivia U. Mason4 6 7 8 1. Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, USA 9 2. Department of Marine Science, Marine Science Institute, University of Texas at Austin, Port 10 Aransas, TX, USA 11 3. School of Biosciences, University of Exeter, Exeter, UK 12 4. Department of Earth, Ocean, and Atmospheric Science, Florida State University, Tallahassee, 13 FL, USA 14 5. Department of Oceanography and Coastal Sciences, Louisiana State University, Baton Rouge, 15 LA, USA 16 6. Louisiana Universities Marine Consortium, Chauvin, LA USA 17 7. Architecture et Fonction des Macromolécules Biologiques, CNRS, Aix-Marseille Université, 18 13288 Marseille, France 19 8. INRA, USC 1408 AFMB, F-13288 Marseille, France 20 9. Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia 21 22 *Correspondence: 23 JCT [email protected] 24 BJB [email protected] 25 26 27 28 Running title: Decoding microbes of the Dead Zone 29 30 31 Abstract word count: 250 32 Text word count: XXXX 33 34 Page 1 of 31 bioRxiv preprint doi: https://doi.org/10.1101/095471; this version posted June 12, 2017.